595 results about "Synergy" patented technology

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention discloses a tail water treatment method and system based on industrial aquaculture of South America white shrimps, and relates to the technical field of aquaculture tail water treatment.The method comprises the steps that a tail water purification integrated device containing a microfilter, a protein separator and other components is obtained, and pretreatment, biological treatment and deep treatment nodes of the tail water treatment process are extracted; corresponding nodes and device components are correlated and mapped to obtain three corresponding processing channels, then tail water component data are collected, control parameters are analyzed based on the three processing channels, and tail water closed-loop processing is carried out. The technical problems that an existing treatment mode for the industrial aquaculture tail water of the South America white shrimps is difficult to achieve standard treatment, high in treatment cost and poor in synergism of all links are solved, and the technical effects that the industrial aquaculture tail water of the South America white shrimps is efficiently treated, the treatment cost is reduced, operation is more convenient and faster, and the synergism of all links is better are achieved.

The invention relates to the technical field of functional food, in particular to immune regulation type prebiotic syrup with synergy of galactomannan. The active components of the composite solid beverage are prepared from the following raw materials in percentage by weight: 20%-35% of fructo-oligosaccharide, 10%-18% of galactooligosaccharide, 8%-15% of stachyose, 3%-7% of xylooligosaccharide, 5%-12% of lactitol, 5%-10% of galactomannan, 2%-5% of yeast beta-glucan and 1%-3% of N-acetylglucosamine. The composition further comprises 4-8% of inulin and 3-6% of resistant dextrin as a matrix filling and slow-release carrier, and the sum of the weight percentages of all the active ingredients and the carrier is 100%. By remodeling intestinal microecology and inhibiting inflammation pathways, the intestinal inflammation state can be remarkably improved, expression of intestinal mucosa tight junction protein is promoted, and an innovative solution is provided for people suffering from chronic bowel diseases and immune imbalance.

The invention relates to the technical field of environmental remediation, in particular to an in-situ soil perfluorinated compound remediation method and system.The method comprises the following steps that electrochemical targeted enrichment is conducted, applying a pulse type direct-current electric field to the anode and the cathode to drive anionic PFAS in the soil to migrate and enrich towards an anode area; in the anode area, the temperature of anode soil is increased, composite peroxide and a chemical activator are continuously injected, and perfluorinated compounds enriched in the anode are oxidized and degraded; and microbial polishing and ecological restoration: stopping the electric field, carrying out post-treatment on the anode region, adding a microbial agent and a nutritional agent into the anode region, and carrying out biodegradation on residual perfluorinated compounds or degradation intermediate products thereof. Through systematic design of physical field guidance, targeted energy injection, multiple catalytic oxidation and ecological function reconstruction, in-situ soil treatment is achieved, and the core problem in PFAS in-situ remediation is precisely solved.

The invention discloses a preparation method of a liver protection medicine composition containing dihydromyricetin, and relates to the technical field of medicine preparation.According to the method, dihydromyricetin, puerarin, pueraria flower extract, corn oligopeptide, pyrroloquinoline quinone disodium salt, curcumin and ganoderan are used as raw materials; dihydromyricetin and puerarin are stirred, homogenized and dried in a mixed solvent to prepare the liver protection medicine composition, wherein the mass ratio of dihydromyricetin to puerarin is 1: (0.5-2). A multi-target liver protection system is constructed through cooperation of multiple components, the problems that a traditional liver protection medicine is poor in component solubility, easy to degrade in activity and weak in synergistic effect are solved, the bioavailability and stability of the product are improved, the process is simple and controllable, the preparation method is suitable for industrial production and can be prepared into oral preparations such as tablets and oral liquid, and the preparation method is suitable for industrial production. The composition is suitable for protection of alcoholic and chemical liver injury.

The invention relates to the technical field of cosmetics, in particular to a sensitive skin care composition for soothing nerves and a preparation method thereof. According to the sensitive skin care composition capable of soothing the nerves, provided by the invention, the acetyl zingerone clathrate compound, the strawberry fruit extract and the 4-tert-butyl cyclohexanol are compounded, and through the dual-channel synergistic effect of transient inhibition of neural signals and long-acting repair of a cell microenvironment, the synergy of immediate soothing and long-term barrier repair is realized; the vicious circle of sensitive skin is effectively blocked.

The invention discloses a nano-drug delivery system for treating myocardial fibrosis as well as a preparation method and application of the nano-drug delivery system, and belongs to the technical field of biological medicines. According to the system, ZIF-8 is taken as a core carrier, the loading efficiency of pirfenidone is improved through high specific surface area and adjustable aperture, PCM myocardial targeting peptide is covalently coupled on the surface, and the limitation of tissue selectivity is broken through. Through coordination complexation, pi-pi conjugate accumulation and electrostatic interaction synergy, stable drug loading and microenvironment response release are guaranteed; a one-pot method and a carbodiimide method are adopted to wrap PFD and modify PCM on the surface of ZIF-8 to form a nano-drug delivery system; according to the system, the fibrosis process is intervened through multiple mechanisms, the enrichment degree and the acting time of the medicine at the diseased region are improved, the prepared medicine integrates the carrier structure advantage and the targeting peptide function, efficient medicine carrying, precise delivery and controllable release are achieved, a new scheme is provided for myocardial fibrosis treatment, and the system has wide application prospects.

The invention relates to the technical field of daily chemical and skin external preparations, in particular to a six-dimensional synergistic effect-based acne removal and repair composition, which is prepared from the following active ingredients in parts by weight: 1 to 10 parts of sturgeon caviar extract, 0.1 to 10 parts of chlorogenic acid, 0.1 to 20 parts of 4-tert-butyl cyclohexanol and 0.1 to 20 parts of willow extract. The sturgeon caviar extract is prepared by supercritical CO2 fluid extraction, the willow extract is treated by a synthase process, the content of salicin is not less than 1.5 wt%, and at least one active component in the composition is subjected to supramolecular inclusion modification; the preparation method comprises the following steps: sequentially preparing the extracts and components, sequentially mixing, and finally carrying out an inclusion reaction. The composition constructs a full-link acne removal and skin health maintenance system through a six-dimensional synergistic effect of bacteriostasis, oil control, anti-inflammation, allergy relief, cutin dredging and repairing, has the characteristics of efficient acne removal, mildness, safety and low recurrence rate, is suitable for various skin types such as sensitive acne skin, and can be widely applied to acne removal products of various dosage forms.

The invention discloses a special ergothioneine-containing skin repair hydrogel for pets and a preparation method of the special ergothioneine-containing skin repair hydrogel, and belongs to the technical field of pet skin care hydrogels. The invention relates to a skin care product which comprises the following components in parts by weight: 1.6-3.8 parts of a functional active component consisting of ergothioneine, quaternized chitosan and oat beta-glucan, 0.15-0.35 part of a cross-linking agent, 5-10 parts of 1, 3-propylene glycol, 5-10 parts of glycerol, 0.2-0.5 part of a preservative and the balance of water, totaling 100 parts. Through precise compatibility of functional active components and synergistic construction of the ionic cross-linked gel matrix, systematic integration of three effects of oxidation resistance, bacteria resistance and healing promotion is achieved, and the technical problems that an existing pet skin care product is single in function, insufficient in synergy and limited in repairing effect are solved.

The invention provides a pharmaceutical composition for treating non-small cell lung cancer and application of the pharmaceutical composition. The pharmaceutical composition comprises gefitinib, erlotinib, afatinib, neratinib, osimertinib and the like which serve as active ingredients. The pharmaceutical composition disclosed by the invention can simultaneously contain various EGFR inhibitors as active ingredients, and the active ingredients are controlled at ultra-low dosage, so that the pharmaceutical composition not only can ensure the treatment effect, even the drug effect of the pharmaceutical composition is obviously superior to that of a high-dosage EGFR inhibitor independent administration group, but also can ensure the safety, and the pharmaceutical composition is suitable for clinical application. And after the active components are combined, a good synergistic effect is achieved, and more importantly, the occurrence of drug resistance can be remarkably reduced or even resisted. In a lung cancer cell culture experiment, the EGFR inhibitor independent administration group has a drug resistance phenomenon at the earliest 20 days, but the pharmaceutical composition group still has no drug resistance phenomenon after 160 days of administration.

The present invention discloses an anti-inflammatory Lactobacillus acidophilus composition for regulating immune responses, belonging to the fields of microbial technology and biomedicine. The composition comprises live lyophilized powders of Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium longum, together with homologous postbiotic lyophilized powders of the same strains. By combining functionally complementary probiotics with their homologous postbiotics, a full-chain anti-inflammatory system is constructed, covering immune regulation, inflammation suppression, and intestinal barrier repair. The live bacteria enable long-term colonization and microecological regulation, while the postbiotics act rapidly, producing a synergistic effect. The composition is prepared solely from strain-derived fermentation products without exogenous additives, ensuring safety and purity, and provides a stable and efficient solution for inflammation relief and immune function optimization.

The invention provides a composite hydrogel for promoting diabetic bone regeneration, the composite hydrogel is composed of L-histidine modified chitosan, oxidized konjac glucomannan, Zn and nano clay, and in-situ gelation is realized through coordination regulation, interlayer exchange and dynamic covalent bond synergistic effect. Through combined application of coordination regulation, interlayer exchange and dynamic covalent bonds, on-demand staged delivery of various ions is realized. According to the innovative design, the adhesive property and mechanical integrity of the hydrogel at a defect part are maintained, intelligent response can be realized according to the change of a bone microenvironment, and the defect that a single release mechanism in the prior art is difficult to meet the complicated demand of diabetic bone regeneration is overcome.

The present application relates to the field of biological glass, in particular to a kind of composite biological glass and its preparation method and application.The present application uses rhBMP-2, doped antibacterial ion 45S5 bioactive glass and polyethylene glycol-lactide-glycolide polymer compound preparation slow-release antibacterial biological glass, the rhBMP-2 can occur synergistic effect with doped antibacterial ion 45S5 bioactive glass, compared with homologous growth factor can play more optimal synergy with antibacterial ion, so as to have higher antibacterial property and osteogenesis, while having longer slow-release effect in polyethylene glycol-lactide-glycolide polymer.The composite biological glass provided by the present application overcomes the pain points of the prior art for the treatment of oral bone defect, improves the treatment effect, reduces the amount of antibiotic use, and reduces the risk of infection.

According to the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the surface stem cells, the brand new pharmacological action of the dipsacus asper saponin VI in targeted promotion of the differentiation of the epidermal stem cells is found for the first time, the cognitive limitation of the prior art is broken through, a brand new target spot and direction are provided for research and development of innovative drugs for wound repair, and the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the epidermal stem cells is realized. A medicine containing teasel saponin VI is adopted, epidermal stem cells are taken as a core target, wound reepithelialization is accelerated by promoting directional differentiation of the epidermal stem cells, the mechanism is clear, the targeting property is high, a core link capable of directly hitting wound healing is adopted, epidermal stem cell differentiation is regulated in a targeted manner, and meanwhile, the medicine has the synergistic effects of resisting inflammation and promoting angiogenesis; the obvious application potential is realized on refractory wounds such as diabetic feet and pressure sores.

The invention relates to the technical field of oral biological materials, in particular to a self-adaptive anti-caries coating as well as a preparation method and application thereof. The coating is prepared from random and block polymers, and is specifically prepared by polymerizing acid radical-containing functional monomers, phenylboronic acid and derivatives thereof. Construction of the coating depends on specific coordination of a functional group and Ca < 2 + > in enamel, and stable anchoring on the tooth surface is achieved. The core action mechanism is to introduce phenylboronic acid groups to endow the coating with pH sensitive characteristics: in a cariogenic acid environment, biofilm formation is synergistically inhibited through a plurality of mechanisms such as physical barrier, electrostatic repulsion and inhibition of mucoprotein-mediated adhesion. According to the application, the Ca < 2 + > anchoring monomer, phenylboronic acid and the derivative thereof are copolymerized, design is carried out aiming at two key links of protein adsorption and bacterial adhesion, multi-mechanism synergy and environment dynamic response are integrated, and an integrated anti-caries effect from early intervention to long-term protection is realized.

The invention discloses a preparation method and application of dual-network piezoelectric induction hydrogel with a piezoelectric effect, and the method comprises the following steps: taking ACG and GelMA as base materials, taking MBA as a cross-linking agent, cooperating with a photoinitiator, and carrying out free radical polymerization to form a precursor solution; and adding BTO nanoparticles, mixing, and curing to obtain the hydrogel. According to the hydrogel disclosed by the invention, the mechanical property and the structural stability of the material are remarkably enhanced through a double-network structure, and the defects that traditional hydrogel is prone to excessive deformation and insufficient in mechanical property are overcome; by introducing a functional component with piezoelectric property, the material can generate a stable electric signal under the action of external force, the local electrophysiological environment of a wound surface is improved, cell proliferation, migration and angiogenesis are promoted, and tissue repair is accelerated; the anti-infection function can be further achieved while electrical regulation and control are achieved, the limitation of a single drug loading or passive protection strategy is avoided, drug resistance and toxic and side effect risks are reduced, and the synergistic effect of antibiosis and regeneration is achieved.

This invention discloses a multi-target synergistic anti-liver cancer medical food granule and its preparation method. The granule uses medicinal and edible homologous foods and novel resource foods as raw materials, and is composed of curcumin extract, esterified fat-soluble EGCG, Moringa leaf powder, yeast β-glucan, Astragalus extract, Schisandra extract, Salvia miltiorrhiza extract, Poria cocos powder, resistant dextrin, and erythritol in a specific ratio to construct a three-dimensional synergistic system of "direct killing-immune regulation-liver protection," which can induce ferroptosis in liver cancer cells, inhibit protective autophagy, activate the body's immunity, and protect liver cells. This invention employs directional extraction, multi-layer coating, and high-pressure fusion granulation technology. Nanoparticle liposome encapsulation, esterification modification, and pH-responsive enteric coating improve the stability and bioavailability of the components, solving the problems of poor water solubility, low absorption, and easy degradation of natural active ingredients. The granule has clear efficacy, high safety, and stable process, and can be used as a medical food for adjuvant therapy and nutritional support during the recovery period of liver cancer.

The invention discloses oral multi-effect analgesic and anti-inflammatory gel and a preparation method thereof, and belongs to the technical field of medical gel. The gel is prepared from mussel mucin grafted hydroxypropyl chitosan, a poly (N-isopropylacrylamide)-co-acrylic acid copolymer, quercetin-zinc coordination nanoparticles, a local anesthetic compound wrapped by temperature-sensitive lipidosome, a gamma-polyglutamic acid-catechin gel matrix, aloe polysaccharide, dipotassium glycyrrhizinate, and sodium hyaluronate and potassium sorbate which can be selectively added, wherein the local anesthetic compound is prepared from the mussel mucin grafted hydroxypropyl chitosan, the poly (N-isopropylacrylamide)-co-acrylic acid copolymer, the quercetin-zinc coordination nanoparticles, the temperature-sensitive lipidosome, the gamma-polyglutamic acid-catechin gel matrix, the aloe polysaccharide and the dipotassium glycyrrhizinate. During preparation, part of the components and water are stirred to obtain a mixed solution, and then the other components are added and stirred into gel. According to the gel, the leakage rate of local anesthetics is reduced by stabilizing the liposome through the zinc ions, the quercetin adjusts the phase transition temperature of the liposome to realize preferential drug release at the pain part, the mussel mucin and the copolymer form a reversible hydrogen bond network to enhance the mucous membrane adhesive force, and the inflammatory environment triggers the gel permeation drug release, so that the gel has the advantages of multiple-effect synergy, strong adhesion and the like; the problems that existing oral gel is short in analgesia time and insufficient in anti-inflammation can be effectively solved.

The invention discloses a method for strengthening three-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction in rice field soil through sulfurized zero-valent iron, and belongs to the technical field of rice field soil pollution remediation. The sulfurized zero-valent iron (S-ZVI) is prepared according to the sulfur-iron ratio of 0.1-0.2; the S-ZVI is mixed with the contaminated soil, and rice is cultivated in cooperation with a specific fertilization and irrigation scheme. According to the method for strengthening the triple-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction in the rice field soil through the sulfurized zero-valent iron, by optimizing preparation and application of the sulfurized zero-valent iron, under the synergistic effect of chemical reduction and microbial degradation, the triple-effect synergy of chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction is expected to be achieved, and the effect of strengthening the rice field soil chloramphenicol degradation, rice yield increase and greenhouse gas emission reduction is achieved. And an important means is provided for degradation of antibiotics in rice field soil.

The invention discloses a bactericide composition for preventing and treating earthen archaeological site mushroom diseases and application of the bactericide composition, triazole bactericides and methoxyl acrylate bactericides are used as active ingredients and compounded with a synergist, the mass ratio of triazole to azoxystrobin is 1: 1-1: 9, the content of the synergist is 0.01%-0.1% of the total mass, the content of the methoxyl acrylate bactericides is 0.01%-0.1% of the total mass, and the content of the methoxyl acrylate bactericides is 0.01%-0.1% of the total mass. The composition shows a remarkable synergistic effect, the FIC index is smaller than or equal to 0.5, and the composition has an efficient killing effect on spores, hyphae and sporocarps of common mushrooms in earthen ruins. The composition ensures safety to a ruins body material, shows a color difference value [delta] E < 3, a penetration depth greater than 5 cm, and maintains long-term stability in a humid environment for at least 12 months. The bactericide formula is systematically optimized for earthen ruins mushroom diseases for the first time, and various macro fungi including coprinus brachypoda, coprinus comatus, pleurotus ostreatus and pleurotus citrinopileatus are effectively prevented and treated. Through laboratory screening, synergistic effect testing, permeability and safety evaluation and field application verification, the composition has an excellent prevention and treatment effect.

The invention provides a preparation method of a probiotic fermented rice cake containing prebiotics and metabiotics. The preparation method comprises the following steps: firstly, adding rice flour, prebiotics, metabiotics and probiotics into water, uniformly mixing, carrying out primary fermentation for 1-24 hours, adding saccharomyces cerevisiae, uniformly mixing, carrying out secondary fermentation for 50-120 minutes, and steaming, so as to obtain the product. Wherein the mass ratio of the rice flour to the prebiotics to the metabiotics is 500: (100-300): (5-10). According to the preparation method disclosed by the invention, a three-element synergistic functional system of prebiotics, probiotics and metabiotics is constructed, so that the prepared probiotic fermented rice cake has full appearance and moderate bulkiness, texture key indexes such as hardness and elasticity of the probiotic fermented rice cake can be remarkably optimized, a better eating mouth feel is obtained, the rice cake is endowed with better texture characteristics, and the probiotic fermented rice cake is suitable for being eaten by people. Besides, the flavor of the rice cake can be enriched, the rice cake is endowed with coordinated and pleasant fermentation flavor, and then the sensory quality of the rice cake is synergistically improved.

The invention discloses a compound fluralab chewable tablet and a preparation method thereof, and relates to the technical field of veterinary drug preparations, the chewable tablet is composed of fluralab, pyrantel and moxidectin solid dispersion constructed by poloxamer 407 / lauryl sodium sulfate (or sodium dodecyl sulfate); preferably, the particle size D50 of the dispersion is 5-30 microns, and D90 is less than or equal to 80 microns. The preparation method comprises the following steps: firstly, dissolving moxidectin in the system to form a clear solution, drying under reduced pressure, and grinding and sieving at low temperature to obtain a dispersed material; premixing with microcrystalline cellulose, then mixing with lactose, adding a disintegrating agent and a lubricating agent, and carrying out prepressing-main pressing two-stage tabletting to obtain the tablet. The system can maintain amorphous distribution of moxidectin in the tablet, the content uniformity and dissolution and acceleration stability are remarkably improved, broad-spectrum synergy is formed by the moxidectin, the fluralasodium and the pyrantel, and the moxidectin tablet is suitable for killing fleas and ticks, treating roundworms and hookworms and preventing canine heart filariasis and tubuloidiasis.

The invention provides a fermentation device based on multi-physical field coordination and application thereof. The fermentation device comprises a fermentation tank unit, an annular magnetic field generation unit, a pulsed electric field generation unit and a control unit. The stable and energy-free background magnetic field is provided through the permanent magnet array, the background magnetic field and the pulsed electric field form synergy, the metabolism efficiency of microorganisms is remarkably improved, the operation cost is low, the device structure is simple, the device can be flexibly deployed, and the device is suitable for large-scale deployment and transformation on an industrial fermentation tank needing long-term and stable operation; a static background magnetic field generated by the permanent magnet is highly stable, is not influenced by fluctuation of a power grid, and is mutually independent and non-interfering with the distribution of a pulse electric field, so that the reliability and repeatability of the process are ensured.

The invention relates to a lycium barbarum fermented product with a liver protection function as well as a preparation method and application thereof, and belongs to the technical field of fermentation. The fermentation liquor is prepared by taking a specific traditional Chinese medicine compound consisting of radix puerariae, semen hoveniae, fructus lycii, rhizoma polygonati, flos chrysanthemi, pericarpium citri reticulatae and cordyceps militaris as a matrix through a double-bacterium synergistic fermentation process. The preparation method comprises the following steps: firstly, pre-fermenting a compound extract by using Akkermansia muciniphila bacteria, enriching propionic acid and optimizing the matrix; the preparation method comprises the following steps: carrying out primary fermentation by using lactic acid bacteria combined with Lactobacillus paracasei E10, L. paracasei JN-1 and L. paracasei JN-8 (in a volume ratio of 3: 3: 2), and carrying out secondary fermentation by using lactobacillus combined with Lactobacillus paracasei E10, L. paracasei JN-1 and L. paracasei JN-8 (in a volume ratio of 3: 3: 2). The traditional Chinese medicine composition realizes double synergy of efficient conversion of traditional Chinese medicine active ingredients and intestinal-hepatic axis regulation and control, and is suitable for prevention and adjuvant therapy of alcoholic liver injury and non-alcoholic fatty liver disease.

The invention provides a method for treating AR negative TNBC through combination of quercetin and enzalutamide, the quercetin up-regulates the AR expression level by inhibiting a high-expression solute carrier SLC7A5, so that tumor cells which are not sensitive to enzalutamide originally obtain drug sensitivity again; the combined use of an AR antagonist enzalutamide (1-80 [mu] M) can cooperatively block an AR signal channel and significantly inhibit cell proliferation (the inhibition rate of drug combination is 70%, Plt, 0.01 higher than that of a single drug). In-vitro experiments prove that the scheme has a synergistic effect (the effect is optimal when the mass ratio is 1: 1-5: 1) in MDA-MB-231 cells, and an animal model shows that the tumor volume inhibition rate reaches 70% or above. Safety evaluation shows that the drug combination does not cause abnormity of serum biochemical indexes (ALT / AST / BUN / CREA) or damage of main organs and tissues. The invention further provides a preparation method of an oral preparation (tablets / capsules / nanoparticles) containing quercetin (50-500 mg / day) and enzalutamide (40-160 mg / day), and a new strategy is provided for reversing AR-TNBC drug resistance.

The invention discloses a composition for reducing scalp grease and application thereof. The invention discloses a functional composition which comprises an effective amount of betulin and glycyrrhizic acid, and the betulin and the glycyrrhizic acid achieve a remarkable effect of reducing scalp grease through mutual synergy and synergism. According to the technical scheme, a brand-new and efficient solution is provided for scalp grease regulation and control.

This invention belongs to the fields of biotechnology and genetic engineering, and relates to a dual-enzyme fusion protein resistant to hydrogen peroxide inhibition, a polynucleotide, an expression vector, and its applications. The dual-enzyme fusion protein is composed of a glutamate oxidase mutant, a catalase mutant, and a linker peptide; the amino acid sequences of the glutamate oxidase mutant and the catalase mutant are shown in SEQ ID NO. 2 and 4, respectively, in the sequence listing. This invention improves the performance of single enzymes through enzyme molecule modification, achieves spatial synergy through fusion protein design, and enables industrial application through a continuous reaction process; it fundamentally solves the problem of hydrogen peroxide's inhibition of glutamate oxidase products; and the fusion expression simultaneously improves the stability and reusability of the dual enzymes.

This invention discloses a bioactive glass and its preparation method, belonging to the field of bioactive glass technology. It aims to solve the problems of insufficient synergistic effect between antibacterial efficacy and bone-promoting activity in existing materials, as well as the inability to monitor in vivo degradation processes in real time. The bioactive glass comprises 44-48 parts silica; 22-27 parts calcium oxide; 22-27 parts sodium oxide; 5-7 parts phosphorus pentoxide; 2-4 parts europium zinc complex; 10-20 parts chitosan; 1-5 parts lidocaine; and 2-5 parts dilute acetic acid solution. The addition of the europium zinc complex promotes bone formation and enhances antibacterial activity, achieving a highly efficient synergy between anti-infection and tissue regeneration. Simultaneously, it provides optical tracking capabilities, allowing for non-destructive real-time monitoring of the material's degradation behavior and tissue integration in vivo via fluorescence imaging. This invention offers superior material performance and a simple preparation process, providing an innovative solution for the repair of infected bone defects.

The application belongs to the technical field of anti-aging skin care product preparation, and discloses a composition containing a composite exosome-polypeptide, a preparation method thereof and application of the composition in cosmetics. The composition contains, in terms of mass fraction, 3.0%-7.0% of an exosome-polypeptide composite wrapped by a biomimetic liposome, 4%-9% of a plant active synergistic extract, 15%-20% of a temperature-sensitive moisturizing sustained-release system, 3%-6% of a lipid barrier repair agent, 0.3%-0.8% of an antioxidant stabilizer, and the rest is a basic base; the exosome is composed of membrane pod huangqi root exosome, salvia miltiorrhiza exosome and long life flower exosome; the polypeptide is composed of palmitoyl tripeptide-5 and acetyl hexapeptide-8; and the biomimetic liposome is composed of phosphatidylcholine, ceramide NP and phytosteryl oleate. The application is compounded by the composite exosome-polypeptide-multiple effect component, hierarchical synergy is achieved, multiple-dimensional aging targets are covered, the transdermal rate is improved, the activity is retained for a long time, the barrier repair and skin quality adaptation are considered, and the anti-aging performance is excellent.

This application relates to the fields of chemistry and biomedicine, specifically to a tofenamic acid lipid composition, its preparation method, and its application. A tofenamic acid derivative is obtained by reacting a morpholine solution with tofenamic acid, ethyldimethylaminopropylcarbodiimide, and 4-dimethylaminopyridine, followed by purification. The morpholine in the morpholine solution is an N-alkylmorpholine compound. The tofenamic acid derivative is used to prepare tofenamic acid prodrug liposomes via ethanol injection, which are then used in combination with a STING agonist. The synergistic effect of the combined treatment stems from the dual regulation of the tumor immune microenvironment (TME), overcoming the immune escape induced by STING monotherapy. After STING agonist treatment, the intratumoral COX-2 / PGE2 axis is activated, leading to immunosuppression. The combination of tofenamic acid liposomes and a STING agonist inhibits COX-2, thus relieving this negative feedback loop.