464 results about "Killing rate" patented technology

The invention discloses a method for knocking out a PD-1 gene by utilizing a CRISPR/Cas9 technology to construct an MSLN-targeted novel CAR-T cell. According to the method, a CAR-T cell is synchronously infected with a virus solution carrying sgRNA and Cas9-HF nuclease of a human PD-1 gene by utilizing the CRISPR/Cas9 technology to knock out the human PD-1 gene, so that the MSLN-targeted novel CAR-T cell is obtained. The novel CAR-T cell can be used for preparing a preparation used for treating solid tumors. A preparation method is simple in step, and the obtained CAR-T cell has high killing rate for solid tumor cells.

The present invention provides a composition, comprising: greater than about 0.1% by weight hydrogen peroxide; an aromatic acid component; surfactant; optionally, a solvent; and a carrier. The composition of the invention is useful as a disinfecting composition for killing microorganisms such as bacterium (including Mycobacterium), spores and fungi. The composition provides a pathogenic bacteria kill rate of 99.9% in about 30 seconds when bacteria are exposed to the composition and is effective in providing a Mycobacterium kill of 10⁶ with two minutes or less. Moreover, the compositions of the invention are generally more resistant to catalase deactivation than, for example, an aqueous solution of hydrogen peroxide. The concentration of hydrogen peroxide within the composition may range from about 1% by weight to about 7% by weight and the concentration of aromatic acid component may range from about 0.1% by weight to about 5% by weight. The invention also provides a method for disinfection of a substrate utilizing the composition. The composition of the invention may be used in the foregoing method on a medical instrument, such as an endoscope or the like. Applying the compositions to a substrate may be accomplished in any of a variety of application methods such as by roll coating, dipping, spraying, or rotational tumbling. The composition may be applied to the substrate for a period of time ranging from about 30 seconds to about ten minutes. In this aspect, the invention can further comprise drying the substrate after removing the composition.

The invention discloses a virus killing method and a virus killing system with multiple antivirus engines. The method comprises the following steps: acquiring a virus scanning command; calling a mainantivirus engine of at least two antivirus engines to search and kill an object which the virus scanning command requires to scan; calling an auxiliary antivirus engine in at least two antivirus engines to search and kill the object which the virus scanning command requires to scan, wherein the virus killing rate of the main antivirus engine is more than that of the auxiliary antivirus engine, andthe auxiliary antivirus engine can be other antivirus engines except the main antivirus engine of at least two antivirus engines. Therefore, the method realizes high speed multi-antivirus engines searching and killing on the scanning object basing on the fact that the success ratio of searching and killing is guaranteed.

The invention discloses a manufacturing method of a long-acting mosquito-expelling textile, comprising the following steps: firstly, by taking one or a mixture of polyester fibers, high-density polyethylene and polypropylene as a raw material, mixing the raw material with a hygienic insecticide to prepare master batches; then heating the master batches and drafting the heated master batches to prepare monofilaments or composite filaments; then cooling the monofilaments or the composite filaments to room temperature and weaving the cooled monofilaments or composite filaments into a mesh fabric; and finally manufacturing the mesh fabric into a textile. The textile fabric filaments manufactured with the manufacturing method of the long-acting mosquito-expelling textile contain the hygienic insecticide internally and externally, a fabric woven from the textile fabric filaments contains the hygienic insecticide capable of resisting washing of more than 20 times, is durable for 3-5 years, has a knocking-down rate and a killing rate which can reach more than 95% for mosquitoes and insects, and can effectively protect a human body from being bitten by the mosquitoes and insects.

The invention relates to preparation and application of a sterilization composition containing various effective sterilization components. The sterilization composition contains a guanidine compound, a quarternary ammonium compound, 2-bromine-2-nitro-1,3-propanediol and ethanol, is diluted within a range of 50-1000 times, has the average killing rate of 99.99-99.9999 percent to gonites, bacterialspores, mycobacteria, viruses, fungi and spores thereof, can be used for the public health field, the food processing industry, the medical and health field, the livestock and poultry industry, the aquaculture industry and sterilization treatment on indoor air and in-vehicle air and can also be used for the port field, in particular to ship ballast water treatment, container treatment, industrialcirculating water treatment, swimming pool water treatment, hand and skin mucosa treatment, industrial wastewater treatment, hospital wastewater treatment and antiseptic treatment of a building material.

The invention discloses a composite antibiotic finishing agent which comprises an organic antibacterial active component, an inorganic antibacterial active component, a penetrating agent, an adhesive, a softening agent, a suspending agent and the balance of water, wherein a non-dissolution type polymer antibacterial agent is selected as the organic antibacterial active component, and silver-loaded nanometer titanium dioxide is selected as the inorganic antibacterial active component. The invention also discloses a method for preparing the composite antibiotic finishing agent and a process used for finishing a mucilage glue and cotton fiber interweaved jacquard fabric. The main material of the composite antibiotic finishing agent for textiles is formed by the organic antibacterial active component and the inorganic antibacterial active component, thus the composite antibiotic finishing agent has a high-efficiency inhibiting capacity of more than 20 kinds of harmful bacteria such as escherichia coli, staphylococcus aureus, gonococcus and the like; the bacteria inhibiting rate is higher than 99 percent; the repelling and preventing rate of dust mites, gamasid mites and chigger is higher than 99 percent; the repelling and preventing rate of dust mites, gamasid mites and chigger is higher than 95 percent, and the killing rate is higher than 90 percent after the mucilage glue and cotton fiber interweaved jacquard fabric is washed for 50 times; and the gonococcus, escherichia coli, staphylococcus aureus and the like do not grow on the fabric which is not washed for 30 times, 50 times or 100 times, and the bacteria inhibiting rate is higher than 99 percent.

The invention discloses an acidizing fluid for green algae and a disease infected cell in porphyra haitanensis cultivation and a treating method thereof. A solvent for the acidizing fluid is clean seawater, and solute for the acidizing fluid is at least one of hydrochloric acid and citric acid, wherein the concentration expressed in percentage by weight of the solute in the acidizing fluid is 0.3 to 1.5 percent. The green algae and the disease infected cell can be quickly killed by a treating method of preparing the acidizing fluid, shipping, dipping for 15 to 20 seconds and rinsing, killing effect is better, the killing rate is over 90 percent, and the influence on the growth of the porphyra haitanensis is less. The treating method has the advantages of no restriction of natural condition, labor intensity reduction and the like.

The invention discloses a plant-derived pesticide and its preparation method and use and belongs to the technical field of pesticide preparation. The plant-derived pesticide is prepared from tobacco, common threewingnut root, capsicum, rhizome of Chinese monkshood, pyrethrum, Azadirachta indica, tea leaves, patrinia herb, bitter herbs, wormwood, ash bark, radix sophorae flavescentis, Euphorbia songarica Boiss, Derris fordii Oliv., Chinese prickly ash, purslane, mandala, Anabasis Aphylla L., leaves of Salix babylonica, leaves of Pterocarya stenoptera and water. The plant-derived pesticide is prepared by soup extraction, condensation, drying, crushing, sieving and disinfection. The plant-derived pesticide is nontoxic and harmless, does not produce residues, is harmless, is environmentally friendly, does not pollute the environment, can effectively prevent and control injurious insects such as yellow spider, arrowhead scales, leaf roller moth and fruit fly, has a one-step killing rate of 95.41% or more, has no drug resistance and can be used for 4-6 years.

An improved composition for skin cleansing and protection is disclosed. The composition contains an effective amount of at least one alpha-hydroxyl acid or a pharmaceutically acceptable salt thereof, at least one base, one surfactant and one skin protectant. Various additives and excipients may be included in the formulation. The improved composition disclosed herein achieves a higher bacteria killing rate and shows longer action duration. The disclosed composition is capable of penetrating deep into the skin which allows for delivery of more anti-microbials to sites that are at a higher risk of being infected. Various modifications of the improved composition are also disclosed.

A removable disposable air dryer cartridge is provided for a small enterprise water ozonation system. The cartridge includes an air inlet to receive atmospheric air, a desiccant material to remove moisture, and a dry air outlet for interfacing with the ozonation system to provide dry air to an ozone generator. This facilitates better ozonation of water in the system, since dry air reacts better in an ozone generator, achieving higher concentrations of ozone gas, thus resulting in a better “kill rate” when ozonated water is applied to bacteria. The air dryer can be provided as a chamber of a combined air dryer and water filter cartridge, the other chamber being a water filter chamber having a water filter for extracting large particles of material from the water stream in order to prevent damage to the system.

The invention discloses antibacterial aluminum and a manufacturing method thereof. The manufacturing method comprises the following steps: (1) a pretreated aluminum alloy serves as an anode to form two electrodes with a cathode module, and the anodic oxidation is performed in electrolyte to prepare a porous aluminum oxide film on the surface of the aluminum alloy, wherein the hole way unit cell number on the unit area of the porous aluminum oxide film is 70-100*109/cm2; and the hole way parameters in unit cells are 1-100 microns of the hole depth and 10-50 microns of the aperture; and (2) the aluminum alloy with the porous aluminum oxide film obtained in the step (1) and an electrode module form two electrodes; antibacterial metal is electrolyzed and deposited in hole ways of the porous aluminum oxide film by using deposition liquid; and the particle size of the deposited antibacterial metal is 1-100 nanometers. The manufacturing method of the antibacterial aluminum is simple in process, convenient in operation and low in manufacturing cost; the antibacterial metal is uniformly and stably deposited; the bacterial resisting and killing effect of the aluminum alloy is long in lasting time; and the bacterial resisting and killing rate is high up to reach 99.99%. Dissoluble precipitations cannot be separated out even if long-time use of electrolysis and deposition liquid.

The present invention encompasses a cleaning composition and method for controlling dust mites and allergens using glycol ether, glycol ether ester or a combination thereof. The cleaning composition preferably contains a hydrophobic glycol ether and/or glycol ether ester solution present at a level of about 0.01% to 20% by weight and is an effective miticide with a kill rate of at least 50% after 30 minutes. The cleaning composition may optionally contain: surfactants, corrosion inhibitors, soil and stain resist agents, builder and buffering agents, and a propellant for delivering the composition in aerosol form.

The invention relates to specific chimeric antigen receptor T cells targeting CD19, and a preparation method and clinical application thereof. The present invention constructs a specific chimeric antigen receptor targeting CD19 and immune response cells modified by the chimeric antigen receptor based on a targeted human CD19 single-chain antibody sequence. The novel modified immune response cells can effectively target and attack a plurality of tumor cells, especially tumor cells with positive CD19 expressiion, and can be used to prepare a preparation for the treatment of tumors. The method for preparing the modified immune response cells targeting CD19 is simple, and the obtained modified immune response cells targeting CD19 have a high killing rate on tumor cells. Clinical verification shows that: after a million-grade low-dose back transfusion, patients with recurrent and refractory advanced CD19-positive lymphoma get a significant clinical symptom relief after two weeks of treatment, almost complete relief curative effect is obtained on the 77th day, and no fever caused by cytokine release syndrome and any neurotoxic side effect occur.

The invention discloses a plant-based agricultural insecticide. The plant-based agricultural insecticide is characterized in that raw materials are prepared from multiple plant-based medicines. Compared with the prior art, the plant-based agricultural insecticide has the high killing rate, the long lasting period and the short safety interval, and can ensure the high agricultural product yield, and agricultural products can be safe and non-toxic.

The invention discloses a novel botanical insecticide and its preparation method and application and belongs to the field of insecticide preparation technology. The novel botanical insecticide comprises the following raw materials: ailanthus altissima, castor-oil plant, Chinese prickly ash, sabah veratrum nigrum, tubatoxin, garlic, tarragon, melia azadarach, onion, leaves of cinnamomum burm anii, ash bark, radix sophorae flavescentis, euphorbia soongarica, sophora alopecuroide, pyrethrum, dioscorea zingiberensis, lettuce, mint, wasabi, patrinia herb and water. The novel botanical insecticide is prepared by steps of medical liquid extraction, concentration, drying, crushing, sieving, sterilization and the like. The novel botanical insecticide has advantages of nontoxic component and no residue, is pollution-free, green and environmentally friendly, and will not cause environmental pollution. The novel botanical insecticide can effectively control plant insects such as inchworm, red spider, lopholeucaspis japonica, leaf miner and the like. One-time killing rate reaches 96.42% and above, and the plant insects have no drug resistance.

The hydrogen peroxide air sterilizing liquid consists of hydrogen peroxide 3.0-5.0 wt%, organic acid 0.01-0.10 wt%, EDTA disodium 0.05-0.20 wt%, metal ion complex 0.10-0.40 wt%, non-ionic polyhydroxylated polymer 0.10-0.50 wt%. It is prepared through dissolving EDTA disodium, metal ion complex and non-ionic polyhydroxylated polymer in deionized water, and adding hydrogen peroxide and organic acid via stirring to obtain the stable air sterilizing liquid. The air sterilizing liquid has pH value of 2.0-3.0, hydrogen peroxide reducing rate at 54 deg.c for 14 days of 2.21-2.22 %, mouse acute oral toxicity LD50 greater than 5,000 mg/Kg and mouse acute inhalation LC50 greater than 10,000 mg/cu m. It has high germ killing rate, and is sprayed into air for sterilizing.

The invention discloses a preparation method of high strength double-network antibacterial biological hydrogel. The preparation method comprises (1) mixing modified gelatin and modified histidine, dissolving the mixture in a phosphate buffer solution, and adding a photoinitiator into the solution to cause polymerization under the ultraviolet point light source so that gelatin hydrogel having an imidazole active site is obtained, and (2) soaking the gelatin hydrogel having an imidazole active site in a divalent metal ion salt solution, and then washing the gelatin hydrogel through sterile water to obtain the high strength double-network antibacterial biological hydrogel. The preparation method has the advantages of mild reaction conditions, strong operability and process controllability. The high strength double-network antibacterial biological hydrogel has good mechanical properties, rigidity and toughness, has escherichia coli and staphylococcus aureus killing rates of 99.9% and has good biocompatibility of the base hydrogel.

The invention discloses the field of culture in vitro of immune cells and particularly relates to an animal protein-free immune cell serum-free medium and a culture method thereof, wherein the culture medium is a liquid culture medium. The culture medium per liter comprises the following components: 1400-1500mg of amino acids, 70-75mg of vitamins, 9800-10000mg of salts, 9500-9700mg of organic matters and 76-80mg of proteins. The culture medium prepared by the invention satisfies the special condition required by growth of immune cells well, so that the culture medium is more suitable for growth of immune cells, thereby remarkably improving the cell proliferation efficiency and the biological potency. The culture medium overcomes the defect that in the prior art, batches are different in quality caused by diversity of human or animal serum and component limited donors added into the culture medium, the quality is easy to control, the culture medium is not polluted due to the quality of serum, and the tumor-killing rate effect is good and the cost is low.

The present invention discloses finishing method for nanometer antibiotic fabric. By means of sol-gel technology, silica gel is prepared on fabric with water glass of different modulus as precursor and ammonium salt as catalyst and used in padding fabric to introduce silver antibiotic and endow fabric with excellent lasting antibiotic performance. The fabric may have its 99 % over of colibacillus and Staphylococcus aureus killing rate maintained after being washed for 50 times. The finishing process has few influence on the hand feeling, whitenes and physical and mechanical performance of the fabric, uses no adhesive and dispersant, and has less pollution and low cost.

The invention discloses a human serum-free culture medium and a preparation method thereof. The human serum-free culture medium uses eleven raw materials, namely human serum albumin solution for treatment, human recombinant insulin solution, human transferrin solution, human cholesterol solution, human catalase solution, 2-mercaptoethanol solution, ascorbic acid solution, linoleic acid solution, ethanolamine solution, human vitronectin solution and L-glutamine solution; the added protein and lipid are both from the blood plasma, serum or tissue of human, the protein is pharmaceutical-grade orhighly purified human protein or human recombinant protein, the protein and lipid do not contains any animal component, the other components all meet the United States Pharmacopoeia or national standards; and the human serum-free culture medium is qualified through the cell culture test and is clinical, safe and reasonable human serum-free culture medium. The proliferation rate of the CIK cell cultured and inducted by the human serum-free culture medium is better than that of the CIK cell cultured and inducted by the culture medium with serum, the cell CD3+CD56+ percentage and the killing rate to the K562 leukemic cell are similar to that of the culture medium with serum. By adopting the human serum-free culture medium, the safety and standardization of cell therapy can be increased.

The invention discloses a disinfectant. The disinfectant has a formula consisting of an agent A and an agent B, wherein the agent B comprises the following components in percentage by weight: 90 to 93% of glacial acetic acid, 3 to 5% of one or mixture of sulfuric acid and phosphoric acid, and the balance of water; and the agent B comprises the following components in percentage by weight: 90 to 93% of hydrogen peroxide, 3 to 5% of hydroxyethylidene diphosphonic acid, 0.05 to 0.5% of 8-hydroxyquinoline, and the balance of the water. According to the disinfectant, the formula is adjusted, and thus the stability is higher; in addition, the agent A cannot be frozen at temperature above 0 DEG C; the disinfectant has killing rate being more than 99% to reproductive and respiratory syndrome virus and escherichia coli virus; and the product quality meets National Veterinary Drugs Standard. The disinfectant is suitable for all animals, and can be periodically used for removing scales in a water reservoir and a water pipeline and disinfecting the same, disinfecting the environment of a pig farm, disinfecting a brooder and a thermostat and the like in a chicken farm, cleaning feet of a dairy cattle, cleaning and disinfecting dairy food transportation equipment, and disinfecting the water mass and a tool in aquaculture; and no second pollution is brought.

Highly active, leach-resistant, antimicrobial nonwoven textiles are prepared by treating at least one surface of the nonwoven material with an anionic polyelectrolyte, such as carboxymethyl cellulose, alginic acid, poly(acrylic acid) etc and at least one select quaternary ammonium antimicrobial agent. The textiles of the invention, and products produced from them, exhibit a highly effective quick kill rate, for example a log 4 CFU reduction within a 5 minute contact time, against microbes such as fungi and gram (−) and gram (+) bacteria.

The present invention discloses a quaternary ammonium salt type antibacterial hand washing solution, which comprises, by weight, 0.4-0.8% of sodium lauryl polyoxyethylene ether sulfate, 1-2% of a quaternary ammonium salt, 15-25% of cocamidopropyl betaine, 0.1-0.2% of MES (AESS), 1-2% of coconut oil diethanolamide, 0.05-0.15% of a preservative, 0.1-0.5% of essence, and the balance of distilled water, wherein the pH value is 6.5-7.0. According to the quaternary ammonium salt type antibacterial hand washing solution, the anionic surfactant and the cationic surfactant can stably exist in the compounding system, and the solubility is good when the use amount of the one surfactant is more than the use amount of the other surfactant, such that the coagulation is not easily produced. The quaternary ammonium salt type antibacterial hand washing solution has characteristics of good stability, high escherichia coli killing rate, high staphylococcus aureus killing rate, and foaming property achieving the market level.

The invention relates to a double-specific chimeric antigen receptor T cell as well as a preparation method and application thereof. The double-specific chimeric antigen receptor double-specifically targeting human NKG2DL and human CD47 is constructed on the basis of human NKG2D and human SIRPalpha molecules as well as an immune response cell modified with the double-specific chimeric antigen receptor. The novel modified immune response cell can effectively realize targeting attack of multiple tumor cells, particularly positive tumor cells expressing NKG2DL and CD47 and can be used for preparing preparations for treating tumor. The preparation method of the immune response cell modified with the double-specific chimeric antigen receptor double-specifically targeting NKG2DL and CD47 comprises simple steps, the obtained immune response cell modified with the double-specific chimeric antigen receptor double-specifically targeting NKG2DL and CD47 has high specific killing rate for tumor cells.

The invention discloses a pulse type ultrahigh pressure food processing method, which comprises the following steps: 1) placing food to be processed into a plastic bag for packaging the food under vacuum; 2) placing the vacuum package food containing food in a high pressure container, adding water or oil for sealing, and performing pulse supercharging and discharging for 1 to 4 times, wherein the discharging pressure is 100MPa, 150MPa, 200MPa, 300MPa or 400MPa if the supercharging pressure is 400MPa, the discharging pressure is 100MPa, 150MPa, 200MPa, 300MPa, 400MPa or 500MPa if the supercharging pressure is 500MPa, and the discharging pressure is 100MPa, 150MPa, 200MPa, 300MPa, 400MPa, 500MPa or 600MPa if supercharging pressure is 600MPa; and 3) discharging the pressure completely. In the invention, sterilization and enzyme inactivation are performed at a lower temperature (room temperature) and the color, fragrance, flavor and nutrients are not damaged, so additives are not used. In addition, special stress caused by supercharging and discharging to microorganisms is strengthened, so the microorganisms injuring and killing rate are improved, and the ultrahigh pressure processing time is reduced.