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370 results about "Green algae" patented technology

The green algae (singular: green alga) are a large, informal grouping of algae consisting of the Chlorophyta and Charophyta/Streptophyta, which are now placed in separate divisions, as well as the potentially more basal Mesostigmatophyceae, Chlorokybophyceae and Spirotaenia.

Multi-temporal-spatial-scale cyanobacterial bloom early warning method for middle and large lake and reservoir water areas

The invention discloses a multi-temporal-spatial-scale cyanobacterial bloom early warning method for middle and large lake and reservoir water areas, and belongs to the field of cyanobacterial bloom prediction and risk monitoring. The method comprises the following steps: generating a pixel-level FAI index based on target water area remote sensing data, resampling meteorological data into a pixel level, then constructing a spatial-temporal distribution data set, training an Autoformer-ST-GNN time sequence model to realize FAI index prediction, dividing cyanobacterial bloom levels according to the FAI index prediction, and obtaining a global change trend; water quality monitoring points are arranged in key areas to collect data, historical water quality and meteorological data are utilized to train a DMC-PatchTST model fused with a blue-green algae migration period, and multi-time-scale prediction of the density of blue-green algae at the monitoring points is achieved; and finally, combining the global trend with a monitoring point prediction result to construct a space-time multi-scale cyanobacterial bloom comprehensive early warning system. According to the method, multi-source data and multiple models are fused, so that cyanobacterial bloom time-space multi-scale comprehensive early warning is realized, and the method is accurate and comprehensive.
Owner:ZHEJIANG UNIV

Granibacillus saccharophilus JLB-1 and application thereof

The invention discloses a Granibacillus saccharophilus JLB-1, which is classified and named as Terribacillus saccharophilus JLB-1, is preserved in the China Center for Type Culture Collection on April 16, 2025, and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2025800. The invention also discloses a microbial inoculum prepared from the strain. The invention also discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis in degrading COD and ammonia nitrogen in polluted water and inhibiting blue-green algae. The Granibacillus saccharophilus JLB-1 screened by the invention has efficient degradation capacity on COD (Chemical Oxygen Demand) and ammonia nitrogen in a polluted water body, remarkably inhibits the growth of blue-green algae, can be applied to biological treatment of polluted water bodies such as eutrophication water bodies, black and odorous water bodies and the like, and has very important application value and wide application prospect.
Owner:SANYA INSTITUTE OF NANJING AGRICULTURAL UNIVERSITY +1

Lake cyanobacterial bloom pixel level prediction method based on multi-source data fusion

A lake cyanobacterial bloom pixel level prediction method based on multi-source data fusion belongs to the technical field of algae prediction, and comprises the following steps: collecting lake pixel level multi-source basic data in a satellite image and carrying out preprocessing, calculating an algae index to generate a binary distribution product, carrying out space-time matching according to a zoning factor suitability parameter table, and carrying out prediction according to the zoning factor suitability parameter table. Inverting a blue-green algae proliferation rate and adjusting a factor weight; calculating a pixel comprehensive suitability degree; identifying a hysteresis effect factor through correlation analysis and causal test; screening a high impact factor through feature sorting, constructing a diffusion rule, extracting an initial water bloom pixel and determining a diffusion starting point; and constructing a neighborhood iterative diffusion model by using the space-time dynamic pixel-level suitability matrix, and iteratively simulating and outputting a pixel-level water bloom prediction map. According to the method, through multi-source pixel-level data standardization processing and partition threshold modeling, the coupling diffusion model is optimized in combination with the multi-source data, accurate water bloom prediction is achieved, and the space-time precision and practicability of pixel-level prediction are improved.
Owner:JIANGSU CLIMATE CENT

Spraying and recycling integrated device for leaf fertilizer and disease algae-removing pesticide in ebb-tide interval period for cultivating laver on beach

The invention discloses an integrated device for spraying and recycling foliar fertilizer and disease algae-removing medicine in an ebb-tide interval period for cultivating laver on a beach. The integrated device is designed for solving the problems of poor algae growth, rot and green algae invasion caused by uneven nutrition in a sea area. Universal wheels are arranged at the bottom of a tray, the height of the universal wheels is 30 cm, and the universal wheels adapt to hard mud of the mud flat; mechanical traction and traction round holes in the two sides are matched with a hand pull rod with a hook to achieve manual steering and power-assisted traction. According to the pesticide-fertilizer liquid spraying system, a liquid storage tank is connected with a front-end double-row atomization spray head set through a water pump, spraying is conducted from bottom to top, a raft frame rope net is covered, and the double-face pesticide receiving rate of the algae is increased. And the residual liquid recovery system comprises a flocculent filter pad for primary filtration, a conical bottom precipitation tank for standing and sand removal, a floating filter for three-stage filtration and residual liquid recovery to a liquid storage tank. By means of the device, leaf fertilizer and disease algae removal pesticide spraying and recycling are effectively implemented, the prevention and control efficiency is improved, the environmental risk is reduced, and green algae prevention and control and laver yield and income increase are achieved.
Owner:唐志勇

Green algal bestrophin bicarbonate transporters

Aspects of the present disclosure relate to genetically modified plants and / or algae with increased carbon use efficiency as a result of an increased ability for bicarbonate to cross membranes within plant cells. Other aspects of the present disclosure relate to methods of making such plants and / or algae as well as cultivating these genetically modified plants to increase carbon use efficiency and / or growing these genetically modified algae to increase carbon use efficiency.
Owner:THE UNIV OF YORK +1

Lake and reservoir water source algal bloom risk early warning system based on 16S / 18SrRNA gene expression quantity threshold

The invention belongs to the technical field of lake and reservoir water source risk prediction, and provides a lake and reservoir water source algae bloom risk early warning system based on a 16S / 18SrRNA gene expression quantity threshold, and the method comprises the following steps: S1, collecting a sample from a lake and reservoir water source surface layer water body; s2, using 7-gate water bloom algae specific primers for blue-green algae, green algae, diatom, euglena, dinoflagellate, chrysophyta and cryptoalga; s3, based on the qPCR standard curve, calculating the copy number of the 16SrRNA / 18SrRNA gene of the seven water bloom algae; s4, when the gene expression quantity of a certain algal bloom algae continuously reaches 106-7 copy number / mL for 7-12 days, determining that the algal bloom algae is in a window phase; according to the threshold value of the gene expression quantity of the water bloom algae 16SrRNA / 18SrRNA, the window period of water bloom algae cells can be accurately recognized, so that early warning of the algae bloom risk in the lake and reservoir water source is achieved, and early warning of algae bloom outbreak in the lake and reservoir water source is achieved one week or above in advance.
Owner:INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +2

Blue-green algae cleaning boat and cleaning method

The invention relates to the technical field of blue-green algae cleaning, and discloses a blue-green algae cleaning boat and a cleaning method.The blue-green algae cleaning boat comprises a boat body, a bearing frame, a cleaning assembly, a dumping and collecting box and a power supply driving assembly, and the boat is composed of two suspension bodies to improve buoyancy and stability. The bearing frame is located on the inner side of the ship body and provides a bearing area for installing the cleaning assembly and other related components. The cleaning assembly is located above the bearing frame and used for cleaning and conveying algae pollutants. And the dumping and collecting box is arranged at the tail end of the cleaning assembly and is used for receiving and storing the cleaned and conveyed algae pollutants. The power supply driving assembly provides assembly working power and supports work of the cleaning assembly. The small boat firstly runs to a preset position and is accurately positioned by additionally arranging a built-in navigation system. And after the blue-green algae arrives at the target area, the cleaning assembly starts to work, and the blue-green algae is removed from the water surface through a cutting and conveying mechanism and conveyed into a dumping and collecting box. The cleaned algae are directly conveyed into the collecting box through the conveying device, and the pollutant collecting process is completed.
Owner:MINNAN INST OF SCI & TECH

Determination method of monosaccharide content in seaweed

The invention discloses a method for determining the content of monosaccharide in seaweed, and belongs to the technical field of biological detection. According to the method, an ion chromatography sugar separation system is adopted, a pulse ampere detector is combined, CarboPac PA20 is used as a sugar analysis column, and the eight monosaccharide components in the seaweed can be detected under the limited extraction and purification method. The method is low in detection limit, good in stability and suitable for measuring the content of free monosaccharide and polysaccharide hydrolyzed monosaccharide in common algae (such as brown algae, green algae and red algae).
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Composition capable of resisting ultraviolet damage and application of composition in sunscreen product

ActiveCN120437012ACosmetic preparationsToilet preparationsCornus officinalis fruit extractTyrosine
The invention relates to an anti-ultraviolet-damage composition and application thereof in sunscreen products, and the anti-ultraviolet-damage composition is prepared from the following raw materials in parts by weight: 5-8 parts of oxhide tea leaf extract, 3-7 parts of green alga extract, 1-4 parts of dogwood extract, 8-10 parts of sea-buckthorn extract, 2.5-4 parts of butyrospermum parkii butter and 5-8 parts of camellia oleosa seed oil. All the raw materials have a synergistic effect, and free radicals generated by ultraviolet induction are neutralized, so that oxidative stress is relieved, and skin cells are protected from being damaged; ultraviolet rays are absorbed and are prevented from penetrating through the skin, so that direct damage of the ultraviolet rays to the skin is reduced; the activity of tyrosinase is regulated or a melanin synthesis pathway is blocked, the accumulation of melanin is reduced, and the effects of preventing and improving stains, darkness and the like are achieved.
Owner:WILSON COSMETICS CO LTD

Method for cultivating green algae by recycling laver cultivation net curtain

The invention discloses a method for cultivating green algae by recycling a laver cultivation net curtain, which comprises the following steps: firstly, cultivating laver, and cleaning the cultivation net curtain after the laver cultivation period is ended; then, enteromorpha spp attachment and cultivation nursing are carried out, and when the concentration of enteromorpha spores used for spp attachment is 104-105 / ml, the yield of enteromorpha reaches the highest; by means of the culture method, the optimal enteromorpha attached spore concentration can be obtained, the highest yield is obtained, and meanwhile the utilization rate of a sea area and culture equipment is remarkably increased.
Owner:NINGBO UNIV

Water body purification device for drainage basin treatment

The utility model discloses a water body purification device for basin treatment, which comprises a mounting pipe, the top of the mounting pipe is provided with a purification assembly, the purification assembly comprises a support frame rotationally connected with the outer wall of the top of the mounting pipe, the bottom of the outer wall of the circumference of the support frame is fixedly sleeved with a gear ring, and the outer wall of the circumference of the mounting pipe is fixedly provided with a motor. A gear is coaxially fixed to the output end of the motor, the gear is meshed with the gear ring, a guide groove is fixedly formed in the circumferential outer wall of the mounting pipe, a groove in the guide groove is obliquely and downwards formed, a scraping plate is fixedly connected to the outer wall of one side of the guide groove, and the supporting frame is slidably sleeved with a filter screen. The device is used for treating and purifying blue-green algae in a watershed water body, pumping water from a polluted water area, cleaning the surface of the filter screen through continuous rotation of the filter screen in the water body purification assembly, and purifying and scraping off fine blue-green algae to prepare a biological fertilizer, so that the water body is cleaned, and the water quality is improved at the same time.
Owner:WUHAN ZHONGKE HYDROBOLOGY ENVIRONMENTAL ENG

Blue-green algae bloom remote sensing recognition system based on multi-source satellite and intelligent threshold correction

The invention relates to a cyanobacterial bloom remote sensing recognition system based on a multi-source satellite and intelligent threshold correction, and the system comprises an intelligent image retrieval and screening module which is used for calling a multi-source image according to a daily frequency / weekly frequency period and generating a candidate set; the water body mask and preprocessing module is used for cloud removal, cutting, water body extraction and non-target plaque removal; the water bloom index and threshold value module is used for automatically calculating an ABDI or FAI index according to an image source and carrying out peak height proportion threshold value segmentation; the pattern spot extraction and clustering module is used for extracting algal bloom patches, merging pattern spots and removing small patches; and the standardized product generating and pushing module is used for generating and pushing grids, vectors, daily frequency brief reports and weekly frequency thematic reports. According to the invention, daily frequency / weekly frequency automatic and intelligent identification and achievement business output of cyanobacterial blooms can be realized, and the precision and continuity of remote sensing monitoring are significantly improved.
Owner:北京首创大气环境科技股份有限公司 +1

Blue-green algae image recognition method and system based on hierarchical self-adaption and domain driving

The invention provides a blue-green algae image recognition method and system based on hierarchical self-adaption and domain driving, and the method comprises the steps: enhancing an image through employing an improved dark channel algorithm; constructing a blue-green algae biological attribute text database, and performing synonym replacement and sentence pattern recombination; multi-scale visual features are extracted through a hierarchical adaptive Swin Transform model, and key region characterization is enhanced in combination with dynamic spectrum attention; the text is input into a Bio-ALBERT model, and semantic embedding of field optimization is generated through term mask prediction and attribute relation pre-training; constructing a two-layer heterogeneous graph by using a graph attention interaction network GAIN, calculating a cross-modal association weight through a bidirectional graph attention mechanism, and outputting a cross-modal graph feature; multi-scale cross-modal association is modeled through a hierarchical graph attention fusion mechanism, and a comparison alignment loss optimization model is combined; and high-precision blue-green algae identification is realized. According to the method, through multi-scale perception, domain semantic adaptation and graph structure fusion, the accuracy of blue-green algae detection in a complex environment is improved.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Blue-green algae mud dehydration treatment device based on solar photothermal conversion technology

The utility model discloses a blue-green algae mud dehydration treatment device based on a solar photothermal conversion technology, which belongs to the technical field of blue-green algae dehydration and comprises a dehydration chamber, an evaporation chamber, a collection chamber, a water storage chamber and a photovoltaic power supply system which are communicated in sequence, the dehydration chamber is located below the treatment device, the outer side of the dehydration chamber is connected with an algae mud branch pipe, and an evaporation system is arranged in the dehydration chamber and comprises an underwater heat conduction shell and a floating type composite fabric material; the evaporation chamber is positioned above the whole device; a water collecting top plate is arranged in the evaporation chamber; the floating type composite fabric material is a carbon fiber fabric modified by a nitrogen-doped carbon nano tube; the collecting chamber is positioned on one side of the evaporation chamber; and the water storage chamber is positioned below the collecting chamber. Solar energy is used as clean energy, moisture in blue-green algae is evaporated through the photothermal effect, and therefore efficient treatment of the blue-green algae is achieved, environmental pollution caused by chemical treatment can be reduced, and economic cost of blue-green algae treatment can be effectively reduced.
Owner:YUNNAN UNIV

High-entropy double hydroxide / carbon fiber aerogel electromagnetic wave-absorbing material and preparation method thereof

The invention discloses a high-entropy double hydroxide / carbon fiber aerogel electromagnetic wave-absorbing material and a preparation method thereof, and the method specifically comprises the following steps: (1) activating green algae to obtain carbon fibers, and freeze-drying the carbon fibers to obtain aerogel carbon fibers; (2) immersing the aerogel carbon fiber subjected to heat treatment into a high-entropy layered double hydroxide precursor solution for hydrothermal reaction, and obtaining a high-entropy double hydroxide / carbon fiber aerogel material after the reaction; wherein the temperature of the hydrothermal reaction is 115-120 DEG C, and the reaction time is not less than 5 hours; and (3) freeze-drying the aerogel material obtained in the step (2) to obtain a target product. The high-entropy double hydroxide / carbon fiber aerogel electromagnetic wave-absorbing material disclosed by the invention can realize low-frequency absorption of 2.46 GHz under 2 to 8 GHz, and can realize broadband absorption of 12.44 GHz under 2 to 18 GHz.
Owner:NANJING UNIV OF AERONAUTICS & ASTRONAUTICS

Green alga polysaccharide extract for moisturizing spray and preparation process of green alga polysaccharide extract

The invention discloses a green alga polysaccharide extract for moisturizing spray and a preparation method of the green alga polysaccharide extract, relates to the technical field of plant polysaccharide extraction, and belongs to the patent classification number C08B37 / 00. Comprising the following steps: washing fresh green algae, drying and crushing, adding green algae powder into hot spring water, adding cellulase, pectinase and amylase, heating for enzymolysis reaction, after the enzymolysis reaction is finished, performing enzyme deactivation treatment to obtain enzymatic hydrolysate, filtering the enzymatic hydrolysate through a microfiltration membrane, and collecting microfiltration membrane permeate liquid, thereby obtaining the green algae. And filtering the microfiltration membrane permeate through an ultrafiltration membrane, collecting an ultrafiltration membrane trapped concentrated solution, and drying the ultrafiltration membrane trapped concentrated solution to obtain the green alga polysaccharide extract. According to the method, a membrane technology is adopted, a microfiltration membrane and an ultrafiltration membrane are combined to extract the green algae polysaccharide, the membrane separation and purification process is carried out under the normal temperature condition, the molecular structure of the green algae polysaccharide cannot be damaged, the activity of the green algae polysaccharide is kept, and the method is green, environmentally friendly, energy-saving and consumption-reducing.
Owner:YLI KAZAK AUTONOMOUS PREFECTURE MIYUE SPRING BIOTECHNOLOGY CO LTD

Alga-containing anti-aging repair composition and application thereof

The invention provides an anti-aging repairing composition containing algae and application of the anti-aging repairing composition, the anti-aging repairing composition comprises blue-green algae, tripeptide and a bacillus fermentation product, and the mass ratio of the blue-green algae to the tripeptide to the bacillus fermentation product is (0.01-0.5): (0.1-1): (0.2-2). The composition obtained by compounding the blue-green algae, the tripeptide and the bacillus fermentation product has a synergistic effect in the aspect of resisting senility, especially in the aspect of promoting the secretion amount of collagen, and when the blue-green algae is replaced with other algae or the bacillus fermentation product is replaced with other fungus fermentation products, the effect of resisting senility is achieved. The effects of the composition disclosed by the invention cannot be achieved. Meanwhile, the composition provided by the invention also has multiple effects of repairing a barrier, resisting oxidation, resisting inflammation, promoting intercellular interaction, promoting collagen synthesis, inhibiting collagen degradation and the like.
Owner:GUANGZHOU JIYAN COSMETICS TECH CO LTD

Single-cell green alga and application thereof

PendingCN121109138AWater contaminantsUnicellular algaeHigh concentrationSelenium pollution
The invention discloses a single-cell green alga and application thereof. The name of the single-cell green alga is Coelastrus sp. BS01, the preservation number is CCTCC NO: M 20251671, the preservation date is July 22, 2025, and the preservation unit is China Center for Type Culture Collection in Wuhan University, Wuhan, China. The single-cell green alga can grow in a selenium-polluted water body with relatively high concentration, can efficiently remove selenium in the water body and is enriched in alga cells, so that the single-cell green alga has the application potential of enriching and converting selenium.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Construction method of microalgae-in-situ bacteria strengthening system for degrading naphthenic acid

The invention is applicable to the technical field of industrial wastewater treatment, and provides a construction method of a microalgae-in-situ bacteria strengthening system for degrading naphthenic acid, which comprises the following steps: separating and identifying two strains of in-situ bacteria from a discharge port of an oil-producing wastewater treatment plant, and constructing a three-strain typical stain-resistant green alga-in-situ bacteria co-culture system. The strengthening system can highly tolerate and efficiently degrade naphthenic acid, can effectively remove nitrogen and phosphorus, and has higher biomass and high-value compound yield. The microalgae-in-situ bacterium strengthening system can reduce pollutant discharge and improve the microalgae resource utilization rate, and the microalgae-in-situ bacterium strengthening system can be used as a biological strengthening degradation technology of naphthenic acid and can be suitable for purification treatment of oil production wastewater and petroleum polluted water areas.
Owner:LIAONING NORMAL UNIVERSITY

Oral capsule-like body product for resisting organ failure and aging

The invention provides an oral thylakoid product for resisting organ failure and aging, which is prepared by the following steps: extracting thylakoid from plants including spinach, arabidopsis thaliana, lettuce, cabbage, green algae or wheat seedlings, redissolving the thylakoid into a buffer salt solution A, and carrying out ultrasonic treatment for 10-20 minutes under the ultrasonic power of 200-500W to prepare a suspension; adjusting the concentration of the suspension to 0.5-2 mg / ml, and obtaining a nano capsule-like body with the particle size of 200-500 nm by using a microfluidic technology; the buffer salt solution A is prepared from 10 to 100 mmol / L of 4-hydroxyethyl piperazine ethanesulfonic acid (HEPES), 1 to 2 mmol / L of ethylenediamine tetraacetic acid (EDTA) disodium, 0.5 to 4 mmol / L of magnesium chloride (MgCl2), 1 to 10 mmol / L of ascorbic acid and 100 to 500 mmol / L of sapoeda sodium (SNAC), and the pH value of the buffer salt solution A is adjusted to 7.5 to 8.0; and the concentration of the capsule in the buffer salt solution A is 100-400 mg / mL. According to the product, self QPRT enzyme of the thylakoid is used for efficiently catalyzing de novo synthesis of NAD + of whole body tissues and organs, so that multiple physiological processes such as energy synthesis enhancement, oxidative stress reduction and inflammatory reaction relief are finally realized, organ failure is efficiently reversed, and organ aging is relieved.
Owner:PEKING UNIV

Lake comprehensive ecological risk assessment method and system based on multi-source data fusion

The invention proposes a lake comprehensive ecological risk assessment method and system based on multi-source data fusion, and relates to the technical field of ecological risk assessment, and the method comprises the steps: carrying out the sampling of a preset assessment space range of a target lake, and obtaining the multi-source ecological data of the target lake; obtaining a water environment risk value according to historical water quality monitoring data in the multi-source information database; acquiring ecological water level demand data of the target lake according to the historical water level monitoring data, and acquiring a hydrological situation risk value of the target lake; based on remote sensing image data and historical survey monitoring data of the target lake in the multi-source information database, evaluating lakeside vegetation condition, wetland area, blue-green algae coverage area and benthonic animal monitoring data respectively to obtain a water ecology risk value; and performing weight distribution operation on the water environment risk value, the hydrological situation risk value and the water ecology risk value to obtain a comprehensive ecology risk assessment index corresponding to the target lake. The method is beneficial to the accuracy and systematicness of ecological risk assessment.
Owner:YANGTZE RIVER WATER RESOURCES PROTECTION SCI RES INST +1

Preparation method and application of blue-green algae-based biochar with fenton-like oxidation function

The application discloses a preparation method and application of blue-green algae-based biochar with Fenton-like oxidation function. The preparation method comprises the following steps: (1) selecting an iron-containing flocculant, adding the iron-containing flocculant into natural lake water containing blue-green algae, and then performing sufficient stirring, standing, filtration, drying, crushing and sieving to obtain blue-green algae powder; (2) placing the blue-green algae powder in a tubular furnace for pyrolysis treatment to obtain preformed carbon; then, immersing the preformed carbon in an activating agent solution, performing sufficient immersion, drying, placing the preformed carbon in the tubular furnace for activation treatment, and finally performing heat treatment under air atmosphere to obtain blue-green algae-based biochar material with Fenton-like oxidation function. The application provides application of the prepared blue-green algae-based biochar in dye wastewater treatment, and the dye wastewater treatment is performed in the presence of hydrogen peroxide, and the blue-green algae-based biochar has high degradation removal rate and high stability.
Owner:ZHEJIANG SCI-TECH UNIV

Application of green alga-based carbon quantum dots in preparation of chikungunya virus infection resisting drugs or decontamination agents

PendingCN121818710ACosmetic preparationsToilet preparationsIn vivoChikungunya Virus Infection
The invention relates to the technical field of medicines, in particular to application of green alga-based carbon quantum dots in preparation of chikungunya virus infection resisting drugs or decontamination agents. The green algae-based carbon quantum dots belong to nano materials, and the green algae-based carbon quantum dots prepared by a hydrothermal method are good in water solubility, high in stability and high in biocompatibility. The application of the green alga-based carbon quantum dots in the chikungunya virus infection resisting medicine or decontamination agent is reported, the in-vivo and in-vitro antiviral effect is good, the application is reported for the first time internationally, and a new scheme is provided for prevention and treatment of chikungunya heat.
Owner:THE NAVAL MEDICAL UNIV OF PLA

Green alga protein as well as preparation method and application thereof

The invention discloses a green algae protein and a preparation method and application thereof, and the preparation method comprises the following steps: (1) crushing dried green algae in a crusher to prepare green algae powder; (2) dissolving the green algae powder prepared in the step (1) in a phosphate buffer solution containing NaCl, and performing ultrasonic treatment; (3) placing the product treated in the step (2) in a chromatography cabinet, stirring and centrifuging to obtain a green algae protein crude extract; (4) adding solid (NH4) 2SO4 into the green algae protein crude extract at 4 DEG C until the saturation reaches 10-30%, stirring, centrifuging, and taking supernate; and adding solid (NH4) 2SO4 to reach the saturation degree of 60-80%, stirring, centrifuging, taking the precipitate, re-dissolving in distilled water, dialyzing, and freeze-drying to obtain the green algae protein. According to the method, the sensitization protein in the green algae can be more effectively extracted, and a new internal standard protein is provided for detection of allergens.
Owner:DALIAN POLYTECHNIC UNIVERSITY

Treatment method of fermentation wastewater

The invention discloses a treatment method of fermentation wastewater. The treatment method of the fermentation wastewater comprises the following steps: adding filamentous fungi and green algae into fermentation wastewater generated after fermentation treatment of kitchen garbage, carrying out fermentation culture, then carrying out solid-liquid separation, and extracting grease from the separated solid. The filamentous fungi comprise trichoderma harzianum, and the green algae comprise one or a combination of more selected from chlorella vulgaris, chlamydomonas, haematococcus pluvialis, hylothrix, scenedesmus obliquus and chaetoclada. According to the treatment method of the fermentation wastewater, grease can be produced while the fermentation wastewater is treated, and the treatment cost of the fermentation wastewater is reduced.
Owner:SUZHOU XUNIVERSAL BIOTECHNOLOGY CO LTD

Desert steppe grass interspecific synergistic agent as well as preparation method and application thereof

The invention discloses a desert steppe grass interspecific synergistic agent and a preparation method and application thereof, and relates to the technical field of synergistic agents, the synergistic agent comprises the following raw materials by weight: 30-40 parts of modified cyanobacteria charcoal, 10-15 parts of a microbial fertilizer, 20-30 parts of an organic fertilizer, 10-20 parts of an inorganic fertilizer, 1-2 parts of a plant growth regulator, and 2-5 parts of a water-retaining agent; the modified blue-green algae charcoal is prepared from the following raw materials in parts by weight: 15-30 parts of ferrous sulfate heptahydrate, 15-30 parts of ferric sulfate hydrate, 200-400 parts of water and 10-20 parts of blue-green algae powder. The synergist provided by the invention can effectively improve the growth efficiency of desert grassland grass planting and effectively improve the treatment effect of degraded grassland.
Owner:INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI

Green alga expression method and application of recombinant human serum albumin

The invention discloses a green algae expression method and application of recombinant human serum albumin, and belongs to the technical field of biology. Aiming at the problems of high cost and incomplete post-translational modification of the existing recombinant protein expression system, the invention provides a technical scheme for efficiently expressing human serum albumin by using green algae. The method comprises the following steps: carrying out codon optimization on a human serum albumin gene sequence, introducing a recombinant vector into green algae cells, and screening transgenic algae strains through a culture medium containing a resistance agent, culturing the transgenic algal strain, splitting the algal cells and separating the recombinant albumin. According to the method, efficient production of the human serum albumin is achieved through a green alga expression system, and the product has a correct folding structure and biological activity and is mainly used for preparing medical plasma substitutes, drug carriers and biological agents.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI

Method for increasing fucoxanthine accumulation amount by using microcystis subjected to depolymerization treatment to feed palmarthria formosana

The invention belongs to the technical field of biology, and particularly relates to a method for increasing the fucoxanthine accumulation amount by using microcystis subjected to depolymerization treatment to feed palmarthria formosana. According to the method, microcystis subjected to depolymerization treatment are added in the culture process of the palmitothecium for heterotrophic culture, meanwhile, continuous inflation is conducted, the microcystis subjected to depolymerization treatment are swallowed by the palmitothecium, and the higher fucoxanthine accumulation amount in the palmitothecium is obtained by means of the culture mode. The experiment proves that after the microcystis is subjected to pretreatment and depolymerization, the removal efficiency of the microcystis is higher by the brown algae, the limitation of treatment of harmful cyanobacteria and ingestion of protozoa is made up, and meanwhile, an integrated solution is provided for biological treatment of cyanobacterial bloom and resource utilization of the microalgae; meanwhile, a new way is provided for obtaining fucoxanthine with higher accumulation amount.
Owner:HUBEI ZAOLAN BIOTECHNOLOGY CO LTD

Rapid fattening bait for sea urchins and preparation method thereof

The invention discloses a rapid fattening bait for sea urchins and a preparation method thereof, and belongs to the technical field of aquaculture feeds. The bait is prepared from the following raw materials in parts by weight: 10-25 parts of kelp powder, 10-25 parts of undaria pinnatifida powder, 5-15 parts of ulva pertusa powder, 5-15 parts of mussel powder, 8-20 parts of corn flour, 1.5-4.5 parts of gelatin powder and 30-50 parts of purified water. The sea urchin fattening feed has the beneficial effects that the formula is scientific, the nutrition is comprehensive and balanced, and the sea urchin fattening feed specially aims at the sea urchin fattening nutritional requirements and can remarkably promote rapid development of sea urchin gonads; after the bait enters water, the structure can be kept complete for a long time, the bait is almost free of dissolution loss, the utilization rate of the bait is greatly improved, and water pollution is fundamentally avoided; the bait is soft in texture and easy to gnaw by sea urchins, and the surface of the bait can slowly release food calling substances to attract the sea urchins to gather and eat; in the preparation process, low-temperature mixing and gel shaping are adopted, so that nutrient substances such as heat-sensitive vitamins and unsaturated fatty acids are effectively protected.
Owner:YANTAI VOCATIONAL COLLEGE

Blue-green algae collecting and flocculating device

The utility model relates to the field of blue-green algae treatment equipment, in particular to a blue-green algae collecting and flocculating device which comprises a barrel body, a flocculating agent leading-in assembly, a water pump assembly, an electric box, a first guide pipe, a second guide pipe, a blue-green algae collector, an insertion column, a ladder, a waste algae discharge pipe and a purified water discharge pipe, the flocculating agent collecting assembly with the flowmeter and the pressure controller is arranged, the dosage of a flocculating agent can be accurately controlled according to different flocculation requirements, and the flocculating agent can be uniformly guided into the barrel, so that the flocculation effect is better, and the flocculating agent is not easily wasted; according to the blue-green algae absorption device, blue-green algae can be intensively collected together through pressure in the blue-green algae absorption process and then guided into the flocculation device, absorption can be more thorough, part of blue-green algae is prevented from being precipitated or dispersed in the absorption process, and the treatment effect is better.
Owner:武汉市水务建设工程有限公司