454 results about "Green algae" patented technology

The invention discloses a multi-temporal-spatial-scale cyanobacterial bloom early warning method for middle and large lake and reservoir water areas, and belongs to the field of cyanobacterial bloom prediction and risk monitoring. The method comprises the following steps: generating a pixel-level FAI index based on target water area remote sensing data, resampling meteorological data into a pixel level, then constructing a spatial-temporal distribution data set, training an Autoformer-ST-GNN time sequence model to realize FAI index prediction, dividing cyanobacterial bloom levels according to the FAI index prediction, and obtaining a global change trend; water quality monitoring points are arranged in key areas to collect data, historical water quality and meteorological data are utilized to train a DMC-PatchTST model fused with a blue-green algae migration period, and multi-time-scale prediction of the density of blue-green algae at the monitoring points is achieved; and finally, combining the global trend with a monitoring point prediction result to construct a space-time multi-scale cyanobacterial bloom comprehensive early warning system. According to the method, multi-source data and multiple models are fused, so that cyanobacterial bloom time-space multi-scale comprehensive early warning is realized, and the method is accurate and comprehensive.

The remediation method for the moderately and severely cadmium-polluted rice field aims at strictly controlled cadmium-polluted rice fields, and is divided into two stages of strict control and safe utilization for remediation. In the strict management and control stage, an activating agent is applied to the soil in spring, then microalgae are inoculated, cadmium-enriched plants are planted, the plants are collected after being full of the microalgae, the plants are harvested when the plants grow to the enrichment extreme value, stubbles are reserved for growth, and the steps are repeated till winter; applying an activating agent in winter, planting green manure, and harvesting in spring in the next year; the process is cyclically carried out until the cadmium content of the soil is reduced to a safe utilization level. Entering a safe utilization stage, applying an activating agent in spring, inoculating nitrogen-fixing cyanobacteria, collecting the cyanobacteria after the cyanobacteria overgrow, and repeating the steps until the rice seedlings are transplanted; applying nitrogen-fixing blue-green algae, and planting rice; applying an activating agent after rice harvesting, planting winter green manure, and harvesting in next spring; the process is cyclically carried out until the cadmium content of the soil is reduced to a preferential protection level. The method is low in cost, high in remediation efficiency and good in effect, and a new way is provided for cadmium-polluted rice field remediation.

The invention discloses a Granibacillus saccharophilus JLB-1, which is classified and named as Terribacillus saccharophilus JLB-1, is preserved in the China Center for Type Culture Collection on April 16, 2025, and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2025800. The invention also discloses a microbial inoculum prepared from the strain. The invention also discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis in degrading COD and ammonia nitrogen in polluted water and inhibiting blue-green algae. The Granibacillus saccharophilus JLB-1 screened by the invention has efficient degradation capacity on COD (Chemical Oxygen Demand) and ammonia nitrogen in a polluted water body, remarkably inhibits the growth of blue-green algae, can be applied to biological treatment of polluted water bodies such as eutrophication water bodies, black and odorous water bodies and the like, and has very important application value and wide application prospect.

A lake cyanobacterial bloom pixel level prediction method based on multi-source data fusion belongs to the technical field of algae prediction, and comprises the following steps: collecting lake pixel level multi-source basic data in a satellite image and carrying out preprocessing, calculating an algae index to generate a binary distribution product, carrying out space-time matching according to a zoning factor suitability parameter table, and carrying out prediction according to the zoning factor suitability parameter table. Inverting a blue-green algae proliferation rate and adjusting a factor weight; calculating a pixel comprehensive suitability degree; identifying a hysteresis effect factor through correlation analysis and causal test; screening a high impact factor through feature sorting, constructing a diffusion rule, extracting an initial water bloom pixel and determining a diffusion starting point; and constructing a neighborhood iterative diffusion model by using the space-time dynamic pixel-level suitability matrix, and iteratively simulating and outputting a pixel-level water bloom prediction map. According to the method, through multi-source pixel-level data standardization processing and partition threshold modeling, the coupling diffusion model is optimized in combination with the multi-source data, accurate water bloom prediction is achieved, and the space-time precision and practicability of pixel-level prediction are improved.

The invention discloses an integrated device for spraying and recycling foliar fertilizer and disease algae-removing medicine in an ebb-tide interval period for cultivating laver on a beach. The integrated device is designed for solving the problems of poor algae growth, rot and green algae invasion caused by uneven nutrition in a sea area. Universal wheels are arranged at the bottom of a tray, the height of the universal wheels is 30 cm, and the universal wheels adapt to hard mud of the mud flat; mechanical traction and traction round holes in the two sides are matched with a hand pull rod with a hook to achieve manual steering and power-assisted traction. According to the pesticide-fertilizer liquid spraying system, a liquid storage tank is connected with a front-end double-row atomization spray head set through a water pump, spraying is conducted from bottom to top, a raft frame rope net is covered, and the double-face pesticide receiving rate of the algae is increased. And the residual liquid recovery system comprises a flocculent filter pad for primary filtration, a conical bottom precipitation tank for standing and sand removal, a floating filter for three-stage filtration and residual liquid recovery to a liquid storage tank. By means of the device, leaf fertilizer and disease algae removal pesticide spraying and recycling are effectively implemented, the prevention and control efficiency is improved, the environmental risk is reduced, and green algae prevention and control and laver yield and income increase are achieved.

Aspects of the present disclosure relate to genetically modified plants and / or algae with increased carbon use efficiency as a result of an increased ability for bicarbonate to cross membranes within plant cells. Other aspects of the present disclosure relate to methods of making such plants and / or algae as well as cultivating these genetically modified plants to increase carbon use efficiency and / or growing these genetically modified algae to increase carbon use efficiency.

The invention provides an aeration and oxygenation device for river channel green algae environment treatment, which is applied to the technical field of water ecological treatment, the device walks in a river channel while oxygenation is carried out by arranging an aeration piece and driving a paddle I to rotate through a motor, and green algae in river water is filtered by a filter screen in a cleaning piece by arranging a transmission piece and the cleaning piece, so that the green algae in the river water is removed, and the water quality is improved. When the motor works for aeration, a scraper in the cleaning part is driven by the transmission part to reciprocate to scrape green algae on the filter screen, the green algae in a river channel are cleaned and collected while aeration and oxygenation are achieved, and when the cutting part and the scraper drive a cutter to move, the cutter and the side wall of the filter screen extrude to cut off the green algae, so that collection is facilitated, blockage is avoided, and the service life of the river channel is prolonged. By arranging the collecting piece and the drainage piece, the driving motor drives the second paddle to rotate, water in the collecting piece is driven to flow, and segmented green algae in the filter screen are sucked into the L-shaped channel and intercepted by the inclined filter plate, so that the green algae automatically enter the collecting screen box to be collected.

The invention belongs to the technical field of lake and reservoir water source risk prediction, and provides a lake and reservoir water source algae bloom risk early warning system based on a 16S / 18SrRNA gene expression quantity threshold, and the method comprises the following steps: S1, collecting a sample from a lake and reservoir water source surface layer water body; s2, using 7-gate water bloom algae specific primers for blue-green algae, green algae, diatom, euglena, dinoflagellate, chrysophyta and cryptoalga; s3, based on the qPCR standard curve, calculating the copy number of the 16SrRNA / 18SrRNA gene of the seven water bloom algae; s4, when the gene expression quantity of a certain algal bloom algae continuously reaches 106-7 copy number / mL for 7-12 days, determining that the algal bloom algae is in a window phase; according to the threshold value of the gene expression quantity of the water bloom algae 16SrRNA / 18SrRNA, the window period of water bloom algae cells can be accurately recognized, so that early warning of the algae bloom risk in the lake and reservoir water source is achieved, and early warning of algae bloom outbreak in the lake and reservoir water source is achieved one week or above in advance.

The invention relates to a preparation method of potassium-nitrogen co-doped cyanobacteria-based activated carbon, and relates to the field of biomass activated carbon. According to the application, the blue-green algae is taken as a substrate, is carbonized firstly, and then is co-doped and activated by adopting melamine and potassium carbonate, the process operation is simple and easy to control, the utilization rate is high, the cost is low, the economic benefit is high, and the method has great significance on recycling of the blue-green algae; besides, according to the technical scheme, under the condition of controlling the mass ratio of the carbon powder to the potassium carbonate and controlling the mass ratio of the carbon powder to the melamine, the quantity of micropores of the prepared activated carbon can be reduced and the quantity of mesopores can be increased by increasing the addition amount of the melamine; the pore size distribution of the prepared activated carbon is changed from micropores to mesopores, so that the purpose of adjusting the pore size is achieved, and the prepared activated carbon is larger in specific surface area and more excellent in adsorption performance.

The invention relates to the technical field of blue-green algae cleaning, and discloses a blue-green algae cleaning boat and a cleaning method.The blue-green algae cleaning boat comprises a boat body, a bearing frame, a cleaning assembly, a dumping and collecting box and a power supply driving assembly, and the boat is composed of two suspension bodies to improve buoyancy and stability. The bearing frame is located on the inner side of the ship body and provides a bearing area for installing the cleaning assembly and other related components. The cleaning assembly is located above the bearing frame and used for cleaning and conveying algae pollutants. And the dumping and collecting box is arranged at the tail end of the cleaning assembly and is used for receiving and storing the cleaned and conveyed algae pollutants. The power supply driving assembly provides assembly working power and supports work of the cleaning assembly. The small boat firstly runs to a preset position and is accurately positioned by additionally arranging a built-in navigation system. And after the blue-green algae arrives at the target area, the cleaning assembly starts to work, and the blue-green algae is removed from the water surface through a cutting and conveying mechanism and conveyed into a dumping and collecting box. The cleaned algae are directly conveyed into the collecting box through the conveying device, and the pollutant collecting process is completed.

The invention discloses bacillus velezensis HY16 and application of the bacillus velezensis HY16. The strain preservation number of the bacillus velezensis HY16 is CGMCC (China General Microbiological Culture Collection Center) No.31645. The invention further discloses a preparation method of the bacillus velezensis HY16. The bacillus velezensis HY16 provided by the invention belongs to spore bacteria and has good biological safety, and compared with physical, chemical and other algae control modes, the bacillus velezensis HY16 does not cause secondary pollution, is friendly to the ecological environment and accords with the current environmental protection concept. According to the invention, the bacillus velezensis industrial production and preparation process and the algae control application scheme are simultaneously combined for the first time, the method has huge market transformation potential and application value, and river algae control experiment results show that the algae lysing rate can reach more than 96%. The microbial agent prepared by using the bacillus velezensis HY16 is stable in property, safe, environment-friendly and simple to apply, can be used for effectively performing biological control and remediation on a water body with blue-green algae, and has a wide application prospect and value.

The invention discloses a method for determining the content of monosaccharide in seaweed, and belongs to the technical field of biological detection. According to the method, an ion chromatography sugar separation system is adopted, a pulse ampere detector is combined, CarboPac PA20 is used as a sugar analysis column, and the eight monosaccharide components in the seaweed can be detected under the limited extraction and purification method. The method is low in detection limit, good in stability and suitable for measuring the content of free monosaccharide and polysaccharide hydrolyzed monosaccharide in common algae (such as brown algae, green algae and red algae).

The invention relates to an anti-ultraviolet-damage composition and application thereof in sunscreen products, and the anti-ultraviolet-damage composition is prepared from the following raw materials in parts by weight: 5-8 parts of oxhide tea leaf extract, 3-7 parts of green alga extract, 1-4 parts of dogwood extract, 8-10 parts of sea-buckthorn extract, 2.5-4 parts of butyrospermum parkii butter and 5-8 parts of camellia oleosa seed oil. All the raw materials have a synergistic effect, and free radicals generated by ultraviolet induction are neutralized, so that oxidative stress is relieved, and skin cells are protected from being damaged; ultraviolet rays are absorbed and are prevented from penetrating through the skin, so that direct damage of the ultraviolet rays to the skin is reduced; the activity of tyrosinase is regulated or a melanin synthesis pathway is blocked, the accumulation of melanin is reduced, and the effects of preventing and improving stains, darkness and the like are achieved.

The invention discloses a water body remediation carbon sequestration and sink increasing method based on lanthanum modified bentonite, and belongs to the technical field of water body ecological remediation. Comprising the following steps: 1, isolating a to-be-restored water body, and obtaining current data of the water body; step 2, determining the current input amount of lanthanum modified bentonite and a flocculating agent based on the current data of the water body, and adding the lanthanum modified bentonite and the flocculating agent into the water body to repair the water body: blue-green algae on the surface of the water body settles, the settled blue-green algae die and decompose at the water bottom, and nutrient substances such as greenhouse gas nitrous oxide, methane and nitrogen and phosphorus are generated; aquatic plants are planted in the water body, the dissolved oxygen content of the water body is increased through photosynthesis of the aquatic plants, and release of greenhouse gases nitrous oxide and methane generated in the blue-green algae decomposition process in the second step is inhibited; in a water body environment where aquatic plants are planted, the lanthanum modified bentonite is used for locking phosphorus in a water body and bottom mud, the content of phosphorus in the water body and the bottom mud is reduced, and growth of algae is inhibited.

The invention discloses a method for cultivating green algae by recycling a laver cultivation net curtain, which comprises the following steps: firstly, cultivating laver, and cleaning the cultivation net curtain after the laver cultivation period is ended; then, enteromorpha spp attachment and cultivation nursing are carried out, and when the concentration of enteromorpha spores used for spp attachment is 104-105 / ml, the yield of enteromorpha reaches the highest; by means of the culture method, the optimal enteromorpha attached spore concentration can be obtained, the highest yield is obtained, and meanwhile the utilization rate of a sea area and culture equipment is remarkably increased.

The utility model discloses a water body purification device for basin treatment, which comprises a mounting pipe, the top of the mounting pipe is provided with a purification assembly, the purification assembly comprises a support frame rotationally connected with the outer wall of the top of the mounting pipe, the bottom of the outer wall of the circumference of the support frame is fixedly sleeved with a gear ring, and the outer wall of the circumference of the mounting pipe is fixedly provided with a motor. A gear is coaxially fixed to the output end of the motor, the gear is meshed with the gear ring, a guide groove is fixedly formed in the circumferential outer wall of the mounting pipe, a groove in the guide groove is obliquely and downwards formed, a scraping plate is fixedly connected to the outer wall of one side of the guide groove, and the supporting frame is slidably sleeved with a filter screen. The device is used for treating and purifying blue-green algae in a watershed water body, pumping water from a polluted water area, cleaning the surface of the filter screen through continuous rotation of the filter screen in the water body purification assembly, and purifying and scraping off fine blue-green algae to prepare a biological fertilizer, so that the water body is cleaned, and the water quality is improved at the same time.

The invention provides color-improving high-fat compound feed for fancy carps and a preparation method of the color-improving high-fat compound feed. The color-improving high-fat compound feed for the fancy carp is prepared from the following raw materials in percentage by weight: 10-20% of fish meal, 10-30% of soybean meal, 10-22% of rapeseed meal, 10-20% of peanut meal, 5-8% of fermented cottonseed meal, 1-5% of wheat flour, 5-13% of corn starch, 1-6% of cellulose, 4-5.5% of peanut oil, 4-5.5% of palm oil, 0.03% of capsorubin, 2-4% of green algae, 0.6-2% of shrimp bran powder, 0.02-0.1% of xanthophyll and the balance of water. The compound feed is prepared from the following components in percentage by weight: 1% of calcium dihydrogen phosphate, 1% of vitamin A reinforced refined fish compound premix, 0.05-0.1% of walnut green seedcase powder, 0.015-0.1% of rhus chinensis extract and 0.02-0.1% of icariin. On the basis of meeting the nutritional requirements of the fancy carp, the fancy carp compound feed disclosed by the invention improves the fat level of the feed, and plays the roles of saving protein, reducing the feed cost and improving the coloring effect of fat-soluble carotenoids on the fancy carp in the form of high-fat feed. In addition, fat deposition of the fancy carp is regulated and controlled by adding the rhus chinensis extract, and the problems that the abdominal fat rate of the fancy carp is increased, the body is abnormal and the ornamental value is reduced when the fancy carp is fed with high-fat feed are solved.

The invention relates to a cyanobacterial bloom remote sensing recognition system based on a multi-source satellite and intelligent threshold correction, and the system comprises an intelligent image retrieval and screening module which is used for calling a multi-source image according to a daily frequency / weekly frequency period and generating a candidate set; the water body mask and preprocessing module is used for cloud removal, cutting, water body extraction and non-target plaque removal; the water bloom index and threshold value module is used for automatically calculating an ABDI or FAI index according to an image source and carrying out peak height proportion threshold value segmentation; the pattern spot extraction and clustering module is used for extracting algal bloom patches, merging pattern spots and removing small patches; and the standardized product generating and pushing module is used for generating and pushing grids, vectors, daily frequency brief reports and weekly frequency thematic reports. According to the invention, daily frequency / weekly frequency automatic and intelligent identification and achievement business output of cyanobacterial blooms can be realized, and the precision and continuity of remote sensing monitoring are significantly improved.

The invention provides a blue-green algae image recognition method and system based on hierarchical self-adaption and domain driving, and the method comprises the steps: enhancing an image through employing an improved dark channel algorithm; constructing a blue-green algae biological attribute text database, and performing synonym replacement and sentence pattern recombination; multi-scale visual features are extracted through a hierarchical adaptive Swin Transform model, and key region characterization is enhanced in combination with dynamic spectrum attention; the text is input into a Bio-ALBERT model, and semantic embedding of field optimization is generated through term mask prediction and attribute relation pre-training; constructing a two-layer heterogeneous graph by using a graph attention interaction network GAIN, calculating a cross-modal association weight through a bidirectional graph attention mechanism, and outputting a cross-modal graph feature; multi-scale cross-modal association is modeled through a hierarchical graph attention fusion mechanism, and a comparison alignment loss optimization model is combined; and high-precision blue-green algae identification is realized. According to the method, through multi-scale perception, domain semantic adaptation and graph structure fusion, the accuracy of blue-green algae detection in a complex environment is improved.

The utility model discloses a blue-green algae mud dehydration treatment device based on a solar photothermal conversion technology, which belongs to the technical field of blue-green algae dehydration and comprises a dehydration chamber, an evaporation chamber, a collection chamber, a water storage chamber and a photovoltaic power supply system which are communicated in sequence, the dehydration chamber is located below the treatment device, the outer side of the dehydration chamber is connected with an algae mud branch pipe, and an evaporation system is arranged in the dehydration chamber and comprises an underwater heat conduction shell and a floating type composite fabric material; the evaporation chamber is positioned above the whole device; a water collecting top plate is arranged in the evaporation chamber; the floating type composite fabric material is a carbon fiber fabric modified by a nitrogen-doped carbon nano tube; the collecting chamber is positioned on one side of the evaporation chamber; and the water storage chamber is positioned below the collecting chamber. Solar energy is used as clean energy, moisture in blue-green algae is evaporated through the photothermal effect, and therefore efficient treatment of the blue-green algae is achieved, environmental pollution caused by chemical treatment can be reduced, and economic cost of blue-green algae treatment can be effectively reduced.

The invention discloses a high-entropy double hydroxide / carbon fiber aerogel electromagnetic wave-absorbing material and a preparation method thereof, and the method specifically comprises the following steps: (1) activating green algae to obtain carbon fibers, and freeze-drying the carbon fibers to obtain aerogel carbon fibers; (2) immersing the aerogel carbon fiber subjected to heat treatment into a high-entropy layered double hydroxide precursor solution for hydrothermal reaction, and obtaining a high-entropy double hydroxide / carbon fiber aerogel material after the reaction; wherein the temperature of the hydrothermal reaction is 115-120 DEG C, and the reaction time is not less than 5 hours; and (3) freeze-drying the aerogel material obtained in the step (2) to obtain a target product. The high-entropy double hydroxide / carbon fiber aerogel electromagnetic wave-absorbing material disclosed by the invention can realize low-frequency absorption of 2.46 GHz under 2 to 8 GHz, and can realize broadband absorption of 12.44 GHz under 2 to 18 GHz.

The invention belongs to the technical field of water environment-friendly materials, and particularly relates to a modified kaolin algistat as well as a preparation method and application thereof. The modified kaolin algistat provided by the invention is obtained by modifying kaolin by adopting chitosan quaternary ammonium salt and an allelopathic agent, and more specifically, the algistat is obtained by carrying out ultrasonic treatment on the kaolin at 80-100 DEG C by adopting the chitosan quaternary ammonium salt and linoleic acid. The method has the beneficial effects that the algistat obtained by adopting the method disclosed by the invention has a remarkable algal inhibition effect, and experimental results show that the algistat can prevent secondary outbreak of blue-green algae in water for a relatively long time.

The invention discloses a green alga polysaccharide extract for moisturizing spray and a preparation method of the green alga polysaccharide extract, relates to the technical field of plant polysaccharide extraction, and belongs to the patent classification number C08B37 / 00. Comprising the following steps: washing fresh green algae, drying and crushing, adding green algae powder into hot spring water, adding cellulase, pectinase and amylase, heating for enzymolysis reaction, after the enzymolysis reaction is finished, performing enzyme deactivation treatment to obtain enzymatic hydrolysate, filtering the enzymatic hydrolysate through a microfiltration membrane, and collecting microfiltration membrane permeate liquid, thereby obtaining the green algae. And filtering the microfiltration membrane permeate through an ultrafiltration membrane, collecting an ultrafiltration membrane trapped concentrated solution, and drying the ultrafiltration membrane trapped concentrated solution to obtain the green alga polysaccharide extract. According to the method, a membrane technology is adopted, a microfiltration membrane and an ultrafiltration membrane are combined to extract the green algae polysaccharide, the membrane separation and purification process is carried out under the normal temperature condition, the molecular structure of the green algae polysaccharide cannot be damaged, the activity of the green algae polysaccharide is kept, and the method is green, environmentally friendly, energy-saving and consumption-reducing.

The invention provides an anti-aging repairing composition containing algae and application of the anti-aging repairing composition, the anti-aging repairing composition comprises blue-green algae, tripeptide and a bacillus fermentation product, and the mass ratio of the blue-green algae to the tripeptide to the bacillus fermentation product is (0.01-0.5): (0.1-1): (0.2-2). The composition obtained by compounding the blue-green algae, the tripeptide and the bacillus fermentation product has a synergistic effect in the aspect of resisting senility, especially in the aspect of promoting the secretion amount of collagen, and when the blue-green algae is replaced with other algae or the bacillus fermentation product is replaced with other fungus fermentation products, the effect of resisting senility is achieved. The effects of the composition disclosed by the invention cannot be achieved. Meanwhile, the composition provided by the invention also has multiple effects of repairing a barrier, resisting oxidation, resisting inflammation, promoting intercellular interaction, promoting collagen synthesis, inhibiting collagen degradation and the like.

The invention discloses a single-cell green alga and application thereof. The name of the single-cell green alga is Coelastrus sp. BS01, the preservation number is CCTCC NO: M 20251671, the preservation date is July 22, 2025, and the preservation unit is China Center for Type Culture Collection in Wuhan University, Wuhan, China. The single-cell green alga can grow in a selenium-polluted water body with relatively high concentration, can efficiently remove selenium in the water body and is enriched in alga cells, so that the single-cell green alga has the application potential of enriching and converting selenium.

The invention provides a method for culturing beneficial algae by resource utilization of cyanobacterial blooms, which comprises the following steps: 1, obtaining cyanobacterial (microcystis) blooms, and concentrating the cyanobacterial (microcystis) blooms until the concentration of chlorophyll a is 80-150mg / L; 2, carrying out continuous hydrodynamic disturbance on the high-concentration cyanobacterial bloom, wherein the disturbance intensity is suitable for preventing the cyanobacterial bloom from blackening and stinking; 3, continuously disturbing for 20-60 days to obtain cyanobacterial bloom which turns light yellow green or white; 4, tap water is used for diluting cyanobacterial blooms with changing colors, the concentration during dilution is 0.5-2.3 mL / cm < 2 >, and beneficial algae such as diatom and green algae are inoculated for culture under the conditions of normal temperature and weak light; and 5, when the culture is performed for 12-30 days, the growth density of the beneficial algae reaches the highest peak, and the cell density of the beneficial algae can reach up to 2.5 * 10 < 6 >-5.6 * 10 < 7 > cells / cm < 2 >. According to the method, algal toxin can be removed, beneficial algae such as diatom and green algae can be cultured through resource utilization of the cyanobacterial bloom, and a new way is provided for control and resource utilization of the cyanobacterial bloom.

The invention provides an aerobic composting method and application of reservoir blue-green algae and reed straws, the reservoir blue-green algae, reed straws and the like are used as raw materials, a thermophilic microbial agent is used for performing aerobic composting treatment on the raw materials, and a nano zero-valent iron / biochar composite material is added, so that the aerobic composting effect is achieved. By using the material, the electron transfer rate between microbial species in an aerobic composting system is increased, H2S and other foul gases released in the composting process are efficiently reduced, and the odor emission intensity is effectively reduced while the decomposition speed of organic matters in the aerobic composting process of organic wastes is increased.

The invention is applicable to the technical field of industrial wastewater treatment, and provides a construction method of a microalgae-in-situ bacteria strengthening system for degrading naphthenic acid, which comprises the following steps: separating and identifying two strains of in-situ bacteria from a discharge port of an oil-producing wastewater treatment plant, and constructing a three-strain typical stain-resistant green alga-in-situ bacteria co-culture system. The strengthening system can highly tolerate and efficiently degrade naphthenic acid, can effectively remove nitrogen and phosphorus, and has higher biomass and high-value compound yield. The microalgae-in-situ bacterium strengthening system can reduce pollutant discharge and improve the microalgae resource utilization rate, and the microalgae-in-situ bacterium strengthening system can be used as a biological strengthening degradation technology of naphthenic acid and can be suitable for purification treatment of oil production wastewater and petroleum polluted water areas.

The invention provides an oral thylakoid product for resisting organ failure and aging, which is prepared by the following steps: extracting thylakoid from plants including spinach, arabidopsis thaliana, lettuce, cabbage, green algae or wheat seedlings, redissolving the thylakoid into a buffer salt solution A, and carrying out ultrasonic treatment for 10-20 minutes under the ultrasonic power of 200-500W to prepare a suspension; adjusting the concentration of the suspension to 0.5-2 mg / ml, and obtaining a nano capsule-like body with the particle size of 200-500 nm by using a microfluidic technology; the buffer salt solution A is prepared from 10 to 100 mmol / L of 4-hydroxyethyl piperazine ethanesulfonic acid (HEPES), 1 to 2 mmol / L of ethylenediamine tetraacetic acid (EDTA) disodium, 0.5 to 4 mmol / L of magnesium chloride (MgCl2), 1 to 10 mmol / L of ascorbic acid and 100 to 500 mmol / L of sapoeda sodium (SNAC), and the pH value of the buffer salt solution A is adjusted to 7.5 to 8.0; and the concentration of the capsule in the buffer salt solution A is 100-400 mg / mL. According to the product, self QPRT enzyme of the thylakoid is used for efficiently catalyzing de novo synthesis of NAD + of whole body tissues and organs, so that multiple physiological processes such as energy synthesis enhancement, oxidative stress reduction and inflammatory reaction relief are finally realized, organ failure is efficiently reversed, and organ aging is relieved.

The invention provides a plasmid for purine base mutation in blue-green algae and a preparation method and application thereof, and belongs to the field of bioengineering, the plasmid provided by the invention is named as pCyABE, the plasmid comprises a TadA-nCas9 fusion protein expression cassette and an sgRNA expression cassette, the nucleotide sequence of the TadA-nCas9 fusion protein expression cassette is shown as SEQ ID No.2, the nucleotide sequence of the sgRNA expression cassette is shown as SEQ ID No.2, and the nucleotide sequence of the sgRNA expression cassette is shown as SEQ ID No.2. The nucleotide sequence of the sgRNA expression cassette is as shown in SEQ ID No. 3. The plasmid provided by the invention is particularly suitable for mutation of adenine at any site on a blue-green algae genome into guanine, realizes fixed-point editing of the gene, has the advantages of high editing efficiency and low off-target rate, and has important significance on physiological property research and industrial application of blue-green algae.