159 results about "Metalloproteinase" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention discloses a preparation method of a mesenchymal stem cell exosome based on microcarrier three-dimensional culture and application of the mesenchymal stem cell exosome in anti-aging skin care products. According to the method, a three-dimensional culture system of mesenchymal stem cells is optimized, and the anti-aging function activity of the subsequently separated and purified exosome is remarkably improved by adopting a microcarrier with recombinant collagen as a main component; subsequently, by optimizing an exosome separation and purification technology, and through a specific separation and purification mode and conditions, the exosome with anti-aging active ingredients is further obtained. Experiments prove that the exosome obtained by the method disclosed by the invention can effectively remove metalloproteinase 1 and promote collagen regeneration, has a remarkable anti-aging effect, is suitable for essence, face cream and other dosage forms, and has a wide market prospect.

The invention relates to the technical field of cosmetics, and discloses a cosmetic composition, a preparation method and application of the cosmetic composition in anti-aging and anti-wrinkle skin. The preparation method comprises the following steps: expressing SKAP2 protein through prokaryote escherichia coli, extracting milk exosome, wrapping the SKAP2 protein into the milk exosome, and mixing the milk exosome containing the SKAP2 protein with trehalose dihydrate, water and CMC (Carboxymethyl Cellulose) to prepare a hydrogel preparation which can be used as a cosmetic composition applied to gel coating agents, masks, toner and dry powder patches. The anti-wrinkle effect is achieved by inhibiting the activity of matrix metalloproteinase 1 (MMP1).

According to the recombinant human VII type collagen as well as the preparation method and the application thereof, nucleotide optimized by codons is recombined into plasmids, and the plasmids are expressed in host cells, so that the recombinant human VII type collagen can be efficiently obtained; the recombinant human VII type collagen has collagen activity for promoting cell proliferation and cell adhesion, can inhibit metalloproteinase expression, has a cell repair effect, and can be used as a cell repair product; the method can be widely applied to food, cosmetics, health care products or medical equipment products.

The invention discloses a recombinant VII type collagen as well as a preparation method and application thereof. The recombinant VII type collagen is obtained by repeating a short amino acid sequence GLPGAKGEKGAP for a plurality of times. The secretion yield of the recombinant VII type collagen in a commercial pichia pastoris expression system can be up to about 17.3 g / L, the extracellular secreted protein is basically not degraded in the fermentation process, the purity can reach 90% or above through simple ultrafiltration and decoloration, and industrial mass production can be achieved; and the recombinant VII type collagen has better biological activities of promoting autophagy, improving an endoplasmic reticulum stress state, promoting collagen secretion and inhibiting matrix metalloproteinase from degrading collagen, and shows good tightening and anti-wrinkle effects.

The invention discloses a Bacillus velez, a fermentation product filtrate thereof and a biological product thereof, belonging to the field of microbial technology. A Bacillus velez BTN-HB-F4 strain is obtained by isolating and purifying green prunes from Haba Snow Mountain in Yunnan Province. The fermentation product filtrate thereof has excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging ability, and can achieve good antioxidant effect. Secondly, at various concentrations, the fermentation product filtrate thereof can significantly reduce the proportion of SA-β-Gal-positive cells, can significantly inhibit the gene expression of matrix metalloproteinase 3, and has good anti-aging effect. In addition, the fermentation product filtrate thereof can significantly inhibit the production of zebrafish melanin, and has good whitening effect. The fermentation product filtrate can significantly inhibit the secretion of cellular inflammatory factors IL-6 and TNF-α, and has good soothing effect. Finally, the fermentation product filtrate thereof can also effectively inhibit the growth of pathogenic bacteria Staphylococcus aureus, and has the potential to regulate skin microecology.

The invention belongs to the technical field of active polypeptides and preparation, and particularly relates to an antioxidant and anti-inflammatory cyclopeptide capable of inhibiting matrix metalloproteinase as well as a preparation method and application of the antioxidant and anti-inflammatory cyclopeptide. The cyclic peptide is formed by end-to-end covalent coupling of linear peptide with the amino acid sequence of DEETGEF, has the activity of resisting oxidation, resisting inflammation and inhibiting matrix metalloproteinase, is free of cytotoxicity and skin irritation, and is suitable for being used as cosmetics for resisting skin aging, wrinkles and the like.

The invention discloses application of tea extracellular vesicles in preparation of a product for preventing and / or treating UVB-induced skin photoaging, and belongs to the technical field of application of the tea extracellular vesicles. The tea extracellular vesicles are derived from tea leaves and / or stem leaves and are prepared by a method of combining enzymolysis, multi-stage filtration and ultracentrifugation, and the particle size of the tea extracellular vesicles is mainly distributed in a range of 60-100nm. Experiments show that the tea extracellular vesicles can effectively reduce active oxygen accumulation and lipid peroxidation caused by UVB radiation, repair mitochondrial membrane potential and inhibit expression of matrix metalloproteinase and inflammatory factors, so that photoaging symptoms such as skin wrinkles, erythema and barrier function damage are relieved. The tea extracellular vesicle provided by the invention has the advantages of natural raw materials, good biocompatibility, small side effect and the like, can be used for preparing a plurality of external dosage forms such as gel, cream, spray and the like, and provides a safe and effective new natural product choice for prevention and treatment of skin photoaging.

The invention discloses a rhodotorula mucilaginosa strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa BTN-HB-L28 strain is separated and purified from meconopsis in Lijiang of Yunnan, can be used in the field of cosmetics, has the effects of resisting skin oxidation, resisting aging, repairing skin and relieving skin, can be used as an active matter in skin care products, and has practical application value. The fermentation product filtrate of the strain has excellent HAS inhibition ability, and can significantly inhibit the expression of matrix metalloproteinase 3; the fibroblast proliferation can be obviously promoted under various concentrations; the proportion of SA-beta-Gal positive cells can be remarkably reduced under various concentrations, and the anti-aging effect is achieved; the antioxidant composition has remarkable DPPH and ABTS free radical scavenging capacity and has a good antioxidant effect; the secretion amount of cell inflammatory factors NO, IL-6 and TNF-alpha can be remarkably inhibited, and the good relieving effect is achieved.

The invention provides a whitening and anti-aging extracellular vesicle compound as well as a preparation method and application thereof. The extracellular vesicle compound comprises extracellular vesicles derived from mesenchymal stem cells and active ingredients loaded in the extracellular vesicles, the active component comprises any one or a combination of at least two of retinoic acid, arbutin, panthenol, nicotinamide, ectoine, vitamine, hyaluronic acid, vitamin C, resveratrol or retinol; the ratio of the extracellular vesicles to the active ingredients is (1-10) * 10 < 8 >: (0.01-1) mg. The skin-care active ingredients are entrapped in the extracellular vesicles derived from the mesenchymal stem cells, so that the delivery efficiency of the skin-care active ingredients can be remarkably improved, the direct irritation of free components to the skin can be reduced, the generation of matrix metalloproteinase-1 (MMP-1) and melanin can be effectively inhibited, the light aging process of the skin can be delayed, and the skin-care composition has excellent whitening and anti-aging effects.

The invention discloses a matrix metalloproteinase-responsive multifunctional bionic ferritin nano material as well as a preparation method and application thereof. The method comprises the following steps: modifying melittin on the surface of a ferritin nanoparticle through a genetic engineering method, connecting by using a linker containing a matrix metalloproteinase 9 (MMP-2 / 9) restriction enzyme cutting site, expressing the obtained recombinant ferritin (SEQ ID NO: 6) in escherichia coli, and self-assembling to form the enzyme response functionalized bionic nanoparticle. According to the method, melittin can be connected to ferritin nanoparticles in series, the toxicity of melittin is remarkably reduced, and the melittin responds to matrix metalloproteinase in a tumor microenvironment so as to kill tumor cells; meanwhile, intravenous administration of melittin can be realized, and the melittin injection has a good treatment effect on in-situ and metastatic tumors.

The invention belongs to the technical field of medicinal chemistry, and particularly discloses a novel tubulin and metalloproteinase double-target inhibitor as well as a preparation method and application of the novel tubulin and metalloproteinase double-target inhibitor. A novel tubulin and metalloproteinase double-target inhibitor is obtained as a compound with a targeting function. A verification result shows that the synthesized target compound shows good anti-proliferative activity on tested cancer cells, 15j has the optimal anti-tumor activity, the IC50 value range of the target compound is 0.154-0.296 mu M, and the tumor inhibition rate of the target compound on human lung cancer transplantation tumors is 74.7% (20 mg / kg). Besides, the anti-proliferative activity of the 15j to cis-platinum drug-resistant cells A549 / CDDP is 136.70 times that of the cis-platinum, the anti-proliferative activity of the 15j to adriamycin drug-resistant cells MCF-7 / DOX is 71.14 times that of the adriamycin, and the toxicity of the 15j to human normal cells (LO2 and MCF-10A) is lower than that of positive drugs such as the cis-platinum, the adriamycin and the paclitaxel. Therefore, the compound has the potential purpose of targeted treatment of lung cancer.

The invention relates to a crystalline polymorphic form I, II and III of marimastat, a mixture of the crystalline polymorphic form I and III of marimastat, an amorphous form of marimastat, and processes for their preparation. The invention further relates to a pharmaceutical composition comprising the crystalline polymorphic form I, II or III of marimastat, the mixture of the crystalline polymorphic form I and III of marimastat, the amorphous form of marimastat or a mixture thereof. The invention also relates to the pharmaceutical composition, the crystalline polymorphic form I, II or III of marimastat, the mixture of the crystalline polymorphic form I and III of marimastat, the amorphous form of marimastat or a mixture thereof for use as a medicament, and for use in the prevention and treatment of diseases associated with hyperactivity of extracellular matrix metalloproteinases.

A water-soluble unit dose detergent article including a water-soluble film and a laundry liquid detergent composition, wherein the detergent composition includes:a non-soap surfactant system including anionic non-soap surfactant and nonionic surfactant wherein the anionic non-soap surfactant includes more than 0% and less than 20% by weight of the anionic non-soap surfactant of an anionic non-soap surfactant selected from the group consisting of alkyl sulphate, alkoxylated alkyl sulphate, and a mixture thereof;a metalloprotease; andup to 15% by weight of the composition of water.

The invention discloses a polypeptide composition for promoting skin desquamation and a preparation method and application thereof, the polypeptide composition comprises polypeptides and a polyol preservative, the polypeptides comprise a first polypeptide, a second polypeptide and a third polypeptide, and the first polypeptide is acetyl tetrapeptide-2; the second polypeptide comprises one or more of hyaluronic acid polypeptide, red scorpion toxin and palmitoyl tripeptide-5; and the third polypeptide is prepared from one or more of acetyl octapeptide-3, palmitoyl dipeptide-7, acetyl tetrapeptide-9 and acetyl tetrapeptide-11. The polypeptide composition can significantly up-regulate the gene expression of kallikrein through a synergistic effect and promote old cutin exfoliation; meanwhile, gene expression of metalloproteinase is inhibited, and light aging is delayed. The polypeptide composition disclosed by the invention can be prepared into various cosmetics and has multiple effects of promoting skin desquamation, resisting wrinkles and repairing barriers.

The invention relates to the technical field of marine product processing, in particular to a preparation method of high-activity fish protein powder, which comprises the following steps: cleaning and crushing deep-sea fish, transferring into an enzymolysis tank, adding brown sugar and / or molasses, yeast extract and clean water, and adding metalloproteinase and biological coenzyme for preliminary enzymolysis; bacterial enzymes such as bacillus velezensis, halophilic bacillus and Fengda salt-tolerant microorganisms are introduced for degradation; and mixing the bacterial enzyme degradation liquid with a chitosan solution, and performing spray drying to obtain the high-activity fish protein powder. According to the invention, macromolecular proteins are decomposed into small peptides and free amino acids by utilizing metalloproteinase and biological coenzyme through the synergistic effect of bacteria and enzymes, and microbial fermentation is introduced, so that the small peptides and other organic substances are further decomposed, efficient and comprehensive multi-stage degradation of fish proteins is realized, and release of nutritional active ingredients is maximized; finally, the fish protein powder is protected through a nano drying medium, and the stability and safety of the fish protein powder are improved.

The present invention discloses a multifunctional biomimetic ferritin nanomaterial responsive to matrix metalloproteinases and its preparation method and application. The present invention modifies melittin on the surface of ferritin nanoparticles by a genetic engineering method, connects with a linker containing a matrix metalloproteinase 9 (MMP‑2 / 9) cleavage site, and obtains recombinant ferritin (SEQ ID NO: 6) expressed in Escherichia coli and self-assembled to form enzyme-responsive functionalized biomimetic nanoparticles. The present invention utilizes this method to connect melittin in series on ferritin nanoparticles, significantly reducing the toxicity of melittin, and responding to matrix metalloproteinases in the tumor microenvironment to kill tumor cells; At the same time, intravenous administration of melittin can also be achieved, with good therapeutic effects on both in situ and metastatic tumors.

The invention provides responsive release polypeptide hydrogel. The hydrogel comprises a gel product of a raw material composition containing the following raw material components: (A) a biocompatible polymer containing at least two sulfydryl groups; (B) a matrix metalloproteinase substrate polypeptide; (C) a compound containing a phenolic hydroxyl group structure; (D) a cross-linking agent; and (E) water; the sequence of the matrix metalloproteinase substrate polypeptide is at least one of the following sequences (b1) to (b2): (b1) Cys-Pro-Leu-Gly-Val-Arg-Gly-Arg-Gly-Asp-Ser, (b2) Cys-Pro-Leu-Gly-Val-Arg-Gly-Asp-Ser, (b3) Cys-Pro-Leu-Gly-Val-Arg-Gly (b2), Dnp-Pro-Leu-Gly-Met-Trp-Ser-Arg-C-ys, and (b3), Dnp-Pro-Leu-Gly- The responsive release polypeptide hydrogel can release polypeptide in a cell microenvironment to promote tissue regeneration and repair, and can be used for preparing muscle-bone healing promoting materials and treating ligament injury diseases such as rotator cuff injury.

The present invention provides highly effective and safe means capable of preventing or treating an egg allergy in infants. An oral immunotolerance agent or a food composition for preventing or treating an egg allergy according to the present invention contains a degraded product of heat-denatured egg white with an aspartic protease (e.g. pepsin) or a metal protease (e.g. thermolysin).

The invention discloses a dihydro-beta-agilawood furan type sesquiterpenoids compound as shown in a formula (I) as well as a preparation method and application thereof. The compound provided by the invention can promote cartilage cell proliferation and reverse mRNA expression of symbolic genes Col2A1 and SOX-9 in cartilage cells under inflammation induction; meanwhile, the expression of inflammation-induced cartilage cell matrix metalloproteinase-13mRNA can be down-regulated, the degradation of cartilage cell extracellular matrix can be inhibited, and the cartilage cell metalloproteinase-13mRNA can be used for preparing products for preventing and / or treating diseases or symptoms caused by abnormal cartilage cell activity and / or improving bone and joint movement functions. # imgabs0 #

A composition is provided comprising a cell population and pharmaceuticals derived from human gingival fibroblasts, present in proportions not found in natural gingival tissue, but having a cellular phenotype preferentially selected to express proteins favorable for angiogenesis and anti-inflammatory effects, while reducing the formation of cell populations that promote tumorigenesis and / or metalloproteinases that inhibit tissue regeneration. A method for producing such a composition that increases proliferation several times over compared to previously known methods is provided, and a method for using such a composition in a wide range of human cell therapies is also provided.

The present invention discloses a molecularly imprinted electrochemical ratiometric sensor and a preparation method thereof, belonging to the field of chemical detection technology. The molecularly imprinted electrochemical ratiometric sensor provided by the present invention can be used for bFGF detection. FA and MB are sequentially electropolymerized on an anhydrous metalloproteinase (ANME) surface to prepare a ratiometric sensing interface. With increasing bFGF concentration, the peak current of pFA gradually decreases, while the peak current of pMB remains relatively stable. The ratio of the pFA to pMB currents is used as a signal for bFGF detection. Compared with single-signal sensors, the molecularly imprinted electrochemical ratiometric sensor has higher sensitivity, accuracy, and stability.

A treated human umbilical cord composition for use in the treatment of Perony's disease by in vivo injection in a subject in need thereof using an effective amount of the composition. The composition comprises a water-based human umbilical cord filtrate, wherein endogenous hyaluronic acid (HA) and / or hyaluronic acid, fibronectin, insulin growth factor binding protein-1 (IGFBP-1), sulfated glycosaminoglycan (sGAG), exosomes, interleukin-1 receptor antagonist (IL-1ra), hepatocyte growth factor (HGF), transthyretin, metalloproteinase tissue inhibitor 1 (TIMP-1), and / or a pharmaceutically acceptable salt thereof in an amount effective to reduce the size of Pteroid disease plaques. The present invention relates to an aggregation glycan, or a combination thereof.

This invention discloses a therapeutic molecule, nanoparticles, and their preparation method for locating and stabilizing atherosclerotic plaques. The therapeutic molecule is synthesized based on cholanonic acid and copper peptide, and the nanoparticles are formed by the self-assembly of the therapeutic molecule. The preparation method involves aldehyde modification of both ends of cholanonic acid and coupling it with copper peptide via a Schiff base reaction to obtain a therapeutic molecule with both diagnostic and therapeutic functions. The copper peptide structure in the therapeutic molecule serves as the hydrophilic segment, and the cholanonic acid structure serves as the hydrophilic segment, allowing it to further self-assemble into nanoparticles in water. These nanoparticles exhibit acid responsiveness and can disintegrate in the slightly acidic environment of atherosclerotic sites. The released copper peptide can stabilize plaques by promoting collagen secretion and inhibiting matrix metalloproteinase expression. Simultaneously, the X-ray imaging characteristics of cholanonic acid can be used to locate and diagnose the distribution of plaques, thus achieving integrated stabilization and localization of atherosclerotic plaques.

A composition for inhibiting functions of various types of matrix metalloproteinases in skin and a use thereof. The composition comprises one or more of the following components: epigallocatechin gallate (EGCG), dihydroquercetin, sugarcane polyphenol, coix seed powder, ginseng, or galangal. The composition has the effects of inhibiting expression of various types of matrix metalloproteinases in skin fibroblasts, inhibiting activity of matrix metalloproteinases, improving collagen content, and inhibiting elastase, and does not have obvious side effects. The composition can be used for preparing various cosmetics, functional foods and health care products related to skin aging resistance, and has a wide application prospect.

The present invention relates to a method for modifying meat, including bringing the meat in contact with a Bacillus amyloliquefaciens-derived neutral protease having the following properties (a) to (e), and thermolysin; (a) being an enzyme that hydrolyzes a substrate having a peptide bond to release the peptide, (b) having an optimal pH of 7, (c) being stable at pH=5 to 7, (d) having an optimal temperature of 50°C to 55°C, (e) being a metal protease inhibited by ethylenediaminetetraacetic acid (EDTA), and a composition for modification, containing said neutral protease and thermolysin. According to the present invention, meat can be tenderized conveniently in a short time, and ease of loosening can be imparted to the meat.

An instant fish maw processing technology for delaying degradation of collagen belongs to the technical field of instant fish maw processing, and comprises the following steps: S1, treating fish maw with 0.1% of tea polyphenol, 0.05% of a rosemary extract and 0.2% of chitosan oligosaccharide for 30 min, carrying out enzyme inhibition treatment, and then cleaning and draining for later use; s2, dipping the fish gelatin treated in the step S1 in a solution containing 0.1% of riboflavin and 0.2% of hyaluronic acid, irradiating the fish gelatin with white light for 10s, and then cleaning and draining the fish gelatin for later use; and S3, putting 5-7 parts by weight of fish maw and 65-80 parts by weight of pure milk into a container, sealing, and sterilizing to obtain the instant fish maw. Through enzyme inhibition treatment, the hydrolysis capability of metalloproteinase on collagen is reduced or eliminated, and degradation of the collagen is reduced, so that the nutritional value of the fish maw is maintained; collagen molecules in the fish maw and riboflavin are subjected to a cross-linking reaction to form a stable three-dimensional network structure, so that the acting force between the collagen molecules is enhanced, the structural stability of the collagen is improved, and the degradation of the collagen is delayed.

The present invention relates to a composition for protecting cells from endoplasmic reticulum (ER) stress and oxidative stress induced by ultrafine dust, comprising galangin as an active ingredient. Galangin, which is an active ingredient of the composition for cell protection of the present invention, alleviated an increase in reactive oxygen species, intracellular calcium, and ER stress-related proteins induced by ultrafine dust PM2.5. In addition, galangin counteracted PM2.5-induced cell cycle arrest at the G0 / G1 phase and restored cell cycle regulators and matrix metalloproteinases. As a result, galangin can enhance cell protection against PM2.5-induced ER stress and cell cycle arrest via reactive oxygen species. Therefore, it is suggested that the composition comprising galangin as an active ingredient of the present invention has great potential as a candidate for a preventive agent or a therapeutic agent for diseases related to ER stress and oxidative stress induced by PM2.5.

The invention provides a biomarker composition and an application thereof. The biomarker composition comprises the following proteins: MMP12, UMOD, HbA1c, FGF9, NINJ1 and PRSS8. According to the biomarker composition provided by the invention, through combination of matrix metalloproteinase 12, uromodulin, glycosylated hemoglobin, FGF9, NINJ1 and serine protease 8, the problem of lack of a specific marker for aortic wall structure damage in the prior art is solved.

The application provides an engineered exosome loaded with a CD63 transmembrane domain-matrix metalloproteinase fusion protein and a preparation method and application thereof. The engineered exosome is secreted by a genetically engineered mesenchymal stem cell, has a CD63 derived transmembrane domain anchored on the membrane, and has a matrix metalloproteinase (MMP) loaded on the inner side of the membrane. The fusion protein comprises, from N-terminus to C-terminus, a fourth transmembrane domain and a C-terminal cytoplasmic tail of a human CD63 protein, a flexible linker peptide, and a human MMP without a natural signal peptide. The application realizes efficient and directional loading of MMPs by using a natural biological generation path of the exosome, shields the inhibition of MMPs by metalloproteinase tissue inhibitors in a scar tissue by using the membrane of the exosome, realizes specific activation of MMPs in a target cell, degrades abnormally deposited collagen, adjusts the ratio of type I and type III collagens, and plays an anti-fibrosis and anti-scar formation role. The application also provides a preparation method of the engineered exosome and application thereof in preparation of a medicine for preventing or treating pathological scars.