695 results about "Amylase" patented technology

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention discloses a method for reducing the content of aged starch and soluble miscellaneous sugar in alpha-cyclodextrin, and belongs to the technical field of alpha-cyclodextrin synthesis, the method comprises the following steps: liquefaction, conversion, primary filtration, inclusion, secondary filtration, complexing agent removal and post-treatment; the conversion comprises the following steps: adding alpha-CGTase and isoamylase into the starch liquefied liquid at 38-42 DEG C, adjusting the pH value to 5.30-5.70, and carrying out conversion to obtain a conversion liquid; the primary filtration comprises the following steps: carrying out enzyme deactivation treatment on the conversion liquid, filtering, washing a filter cake with water, and combining the washing liquid with the filtrate to obtain primary filtrate; and the secondary filtration comprises the following steps: filtering the material liquid containing the complex, taking a filter cake, and washing the filter cake with water to obtain the inclusion compound. The preparation method of the alpha-cyclodextrin is improved, so that the alpha-cyclodextrin with high yield and high stability is obtained, and the industrial controllability is realized.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A118 and application thereof. The bacillus velezensis A118 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on colletotrichum capsici. The bacillus velezensis A118 disclosed by the invention also has relatively strong capability of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on chilies, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of pepper diseases is reduced, the pepper growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention discloses an implant-derived exosome which is prepared by the following steps: adding cleaned and crushed colored rice flour into a PBS (Phosphate Buffer Solution) with the pH value of 6-7.4, homogenizing at a high speed, adding alpha-amylase into rice milk, performing enzymolysis for 1-2 hours under the conditions that the pH value is 6-7.4 and the temperature is 90-97 DEG C, filtering, and standing filtrate overnight; carrying out gradient differential centrifugation at 4 DEG C, collecting supernate, filtering, carrying out ultracentrifugation on filtrate at 4 DEG C and 12000-15000g for 1.5-3 hours, and collecting precipitate; and re-suspending the precipitate, enriching by using an MWCO ultrafiltration tube with the molecular weight cut-off of 100kDa, collecting the precipitate at the bottom of the ultrafiltration tube, and re-suspending in a PBS buffer solution, thereby obtaining the product. The exosome can effectively inhibit the generation of inflammatory factors, has a good inhibition effect on various pathogenic bacteria, also has an anti-oxidation effect, and has application prospects in the fields of inflammation resistance, bacteria resistance, oxidation resistance and the like.

The invention relates to the technical field of microorganisms, in particular to bifidobacterium bifidum BGLP1 capable of regulating sugar digestion and producing GLP-1 at high yield and application of the bifidobacterium bifidum BGLP1. The preservation number of the bifidobacterium bifidum BGLP1 is CGMCC (China General Microbiological Culture Collection Center) No.35718, and the bifidobacterium bifidum BGLP1 can be used for preparing the feed additive. The strain has the capability of inhibiting alpha-glucosidase and alpha-amylase; sTC-1 cells are stimulated to secrete GLP-1, and the blood glucose reducing pathway capacity of the intestinal tract is activated; the activity of pancreatic lipase is obviously inhibited, and the dietary fat absorption capability can be effectively reduced; redundant cane sugar is converted into exopolysaccharide, so that the probiotic function capability is improved; the capability of converting white fat into brown fat is promoted; macrophages are activated, and the body immune function is improved. In addition, the bifidobacterium bifidum BGLP1 also has relatively strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body.

The invention discloses a compound bacterial agent for reducing blood sugar and blood fat, application of the compound bacterial agent and a method for preparing a fermented coconut milk beverage from the compound bacterial agent, belongs to the technical field of microorganisms, and particularly relates to the compound bacterial agent for reducing blood sugar and blood fat, the compound bacterial agent comprises fermented lactobacillus mucus AL-8 and lactobacillus paracasei DX-5, and the compound bacterial agent is prepared from the fermented lactobacillus mucus AL-8, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5. The preservation number of the lactobacillus mucus AL-8 is CCTCC (China Center For Type Culture Collection) NO: M20252735; the preservation number of the lactobacillus paracasei DX-5 is CCTCC (China Center For Type Culture Collection) NO: M20252736. According to the invention, the fermented coconut milk beverage is prepared by utilizing the inhibition effect of a blood sugar and blood fat reducing compound bacterial agent consisting of fermented lactobacillus mucus AL-8 and casei paracasei DX-5 on alpha-amylase and pancreatic lipase.

The invention relates to the technical field of adhesives, and particularly discloses a low-formaldehyde environment-friendly adhesive and a preparation method thereof. The invention relates to a low-formaldehyde environment-friendly adhesive which is prepared from the following raw materials in parts by mass: 30-50 parts of modified starch, 15-25 parts of vegetable protein, 5-10 parts of modified nano cellulose, 2-5 parts of a composite cross-linking agent and 80-150 parts of water, the composite cross-linking agent comprises amino silane and polyaspartic acid; the modified starch is prepared by performing graft copolymerization on starch subjected to enzymolysis by alpha-amylase and composite anhydride; the vegetable protein is subjected to alkalization and heat treatment before being used; the modified nano-crystalline cellulose is prepared by performing ring-opening etherification reaction on nano-crystalline cellulose, epoxypropane and ethylene oxide in sequence. The low-formaldehyde environment-friendly adhesive prepared by the invention has relatively good bonding strength and relatively low formaldehyde emission.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A96 and application thereof. The bacillus velezensis A96 disclosed by the invention can resist saline and alkaline, and has a good inhibition effect on fusarium oxysporum. In addition, the bacillus velezensis A96 also has relatively strong capacity of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on cowpeas, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the control cost of cowpea diseases is reduced, the growth of cowpeas is promoted, and the biocontrol bacterium has a good popularization value.

Disclosed is a method for reducing the usage amount of edible fat and oil in a bakery product, the method comprising mixing at least one maltose alpha-amylase and edible fat and oil into dough, and baking same to prepare a bakery product. The usage amount of the edible fat and oil in the dough can be reduced by at least 10 wt % compared with not using the enzyme treatment; and the enzyme may also include cellulase and / or phospholipase. The method does not reduce or substantially reduce the quality of the bakery product, and allows same to have a shelf life of at least 4 days. Also involved is baked fat and oil prepared from the above-mentioned enzyme and edible fat and oil.

The invention discloses a preparation method of highland barley prefabricated powder with a low glycemic index and application of the highland barley prefabricated powder in 3D printed biscuits. According to the method, firstly, cellulase and alpha-amylase are adopted for carrying out enzymatic modification treatment on highland barley flour, the content of anti-digestion starch (RS) is remarkably increased, the content of fast-digestion starch (RDS) is reduced, and therefore the modified highland barley flour with the predicted glycemic index (pGI) reaching the low-GI food standard (GI is smaller than or equal to 55) is prepared, and the dietary requirements of people with different metabolic requirements are met. Further, the modified highland barley powder is compounded with sodium alginate, glutamine transaminase is utilized for inducing and constructing a protein-polysaccharide gel network, the incubated and cross-linked dough is subjected to freeze drying and smashing, and the highland barley prefabricated powder with the low glycemic index is obtained.The 3D printing adaptability, the structural stability and the digestion resistance of the material are effectively enhanced through the method. The highland barley biscuit has the characteristic of low glycemic index, and is suitable for making healthy food for special crowds with diabetes and the like.

The invention discloses low-GI probiotic compound coarse cereal meal replacement powder and a preparation method thereof. The preparation method comprises the steps of raw material and auxiliary material treatment, starch enzymolysis, strain activation culture, fermentation, freeze drying, blending and packaging and the like. The black tartary buckwheat, the black beans, the black sesame, the black rice and the black mulberries are used as main raw materials, good growth conditions are created for lactic acid bacteria by means of an amylase enzymolysis technology, and further the cereals are subjected to synergistic fermentation by inoculating a strain suspension of lactobacillus reuteri and lactobacillus rhamnosus, so that the nutritional value is increased, bioactive metabolites are added, and the nutritional value is increased. And the flavor and taste of the grains are also improved. Compared with common commercially available meal replacement powder, the product realizes directional domestication fermentation of probiotics in a composite coarse cereal environment, so that the product has high viable organism stability, ensures the colonization ability of the probiotics in cereal powder, realizes synergistic improvement of nutrition and flavor, contains certain functional components, and has relatively good sugar controlling and stabilizing ability.

The technical scheme of the invention discloses black fungus protein capable of reducing blood glucose and resisting oxidation as well as a preparation method and application of the black fungus protein. The amino acid sequence of the black fungus protein is as shown in SEQ ID NO. 1. The protein which is separated and identified from the black fungus for the first time and has the effects of reducing blood glucose and resisting oxidation achieves the effect of reducing blood glucose through double inhibition of alpha-amylase and alpha-glucosidase, meanwhile, the protein has remarkable antioxidant activity, animal experiments prove that fasting blood glucose and body weight of a type II diabetes model mouse can be effectively reduced, and the protein has a good application prospect. The compound has high biological activity and safety, and can be used for developing functional products with hypoglycemic and antioxidant activity.

Described herein are genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus amyloliquefaciens QGFCH3-3 as well as a microbial agent and application of the bacillus amyloliquefaciens QGFCH3-3. The preservation number of the bacillus amyloliquefaciens QGFCH3-3 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.34814 The strain has the functions of nitrogen fixation, amylase production, ligninase production, protease production and cellulase production, can effectively promote plant growth, has broad-spectrum antibacterial activity, can significantly inhibit various pathogenic bacteria, and significantly improves the disease control effect of crops.

The invention relates to the technical field of novel fertilizers, and particularly discloses an organic water-soluble fertilizer as well as a preparation method and application thereof. The organic water-soluble fertilizer provided by the invention is prepared from the following components: kitchen waste organic mother liquor, an anti-corrosion synergist, a stress-resistant synergist, a growth-promoting synergist, a dispersing agent and polyethylene glycol-400, the kitchen waste organic mother liquor is prepared by sequentially carrying out wet and hot hydrolysis, amylase enzymolysis, protease enzymolysis and lipase enzymolysis on a kitchen waste solid phase and a kitchen waste liquid phase; the preservative synergist is prepared by mixing a natamycin solution, tea polyphenol and chitosan oligosaccharide; the stress-resistant synergist is prepared by mixing complex enzyme glycoside, a plantain herb extract and gamma-aminobutyric acid; the growth promoting synergist is formed by mixing mineral source potassium fulvate and gluconic acid. The organic water-soluble fertilizer prepared by the invention has good corrosion resistance and product stability, has a good growth promoting effect on crops, and can reduce the number of diseased plants with successive cropping obstacles and improve the yield of the crops in a soil environment with successive cropping obstacles.

The invention provides a preparation method of a compound microbial agent and a pasture fermented feed, and belongs to the technical field of feed fermentation. The complex microbial inoculant disclosed by the invention comprises lactobacillus acidophilus, bacillus siamensis, bacillus amyloliquefaciens and pichia kluyveri. The lactobacillus acidophilus can produce acid and resist acid, and has better antibacterial performance; the bacillus siamensis can degrade lignin and reduce the content of neutral detergent fibers and acid detergent fibers in the fermented feed; the bacillus amyloliquefaciens produces cellulase and amylase with higher activity; the pichia kluyveri can increase the content of crude protein in the fermented feed and improve the nutritional performance of the feed. The four strains have a synergistic effect and jointly ferment forage feed, infectious microbes can be effectively inhibited, the fermented forage feed has excellent color, smell and texture, the palatability of the feed is improved, the appetite of animals is stimulated, and a good growth promoting effect is achieved; the fermented forage feed disclosed by the invention is high in nutritional quality and relatively high in feed utilization rate.

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention provides a starch saccharification reaction state prediction, regulation and control method, which is applied to the technical field of food processing, and comprises the following steps: dividing a reaction system into a plurality of sub-regions, collecting temperature, slurry viscosity, starch particles, enzyme concentration and other sensing data of each sub-region, and calculating a local gelatinization degree index by using a preset gelatinization degree calculation model. According to the method, a saccharification reaction state prediction model is established, saccharification parameters of each sub-region are predicted in combination with the saccharification reaction state prediction model, then a saccharification state prediction result of the whole reaction system is generated, and the type, dosage and time of amylase putting are determined according to the deviation between the prediction result and a preset saccharification target. Therefore, the product quality and the saccharification efficiency are improved.

The invention discloses a method for detecting exogenous amylase derived from aspergillus in honey and application of the method. The method comprises the following steps: screening and determining a characteristic peptide fragment, pre-treating a sample, making a standard curve, separating by using liquid chromatography, detecting by using tandem mass spectrometry, and quantifying by using an internal standard method. The exogenous amylase characteristic peptide fragment derived from aspergillus is FASYTNDIALAK, and the specific peptide fragment derived from aspergillus is FASYTNDIALAK. The invention establishes a quantitative method for detecting the exogenous amylase derived from aspergillus in the honey by liquid chromatography-tandem mass spectrometry, and the quantitative method has better accuracy and sensitivity and is suitable for adulteration identification of the honey. The method has important practical significance for controlling the quality of honey and maintaining the healthy and sustainable development of the bee product industry.

The invention belongs to the technical field of microorganisms and fermentation, and discloses a method for synergistically fermenting ginseng powder through bacteria and enzymes, acetobacter aceti, ginseng spice and application, and the method comprises the following steps: (1) preparing a ginseng powder liquid culture medium; (2) inoculating acetobacter aceti and cellulase, and performing liquid fermentation for 24-36 hours; (3) inoculating amylase, and continuously fermenting for 96-120 hours; and (4) after fermentation is finished, supplementing ethanol until the final volume concentration of ethanol is 45-55%, stirring at normal temperature for 24 hours, standing, settling and filtering to obtain supernate, namely the ginseng spice. According to the preparation method, the earthy smell of the traditional ginseng spice can be remarkably reduced by the obtained ginseng spice through technologies such as bacterium-enzyme synergy and specific microbial fermentation metabolism control, meanwhile, the ginseng spice has typical aroma characteristics and is high in suckability, and meanwhile, the product quality of ginseng spice series cigarettes is effectively improved.

The invention discloses an application of astaxanthin in preparation of a regulator for improving the gentisic acid level. On the basis of non-targeted metabonomics analysis, it is found for the first time that in the SAP pathogenesis process, the level of a flora metabolite, namely gentisic acid (GA), in intestinal contents is remarkably reduced, and in-vivo gentisic acid level can be remarkably callback through astaxanthin intervention. Further research proves that exogenous supplement of gentisic acid can effectively simulate the protection effect of astaxanthin, including improvement of pancreatic tissue pathological damage, reduction of serum lipase and amylase activity, and inhibition of systemic inflammatory response (down-regulation of IL-1beta, IL-6 and TNF-alpha and up-regulation of IL-10). The invention not only discloses a new mechanism that astaxanthin plays a therapeutic role through an'intestinal flora-metabolite 'axis, but also determines that gentisic acid is used as a novel drug target for treating SAP, and constructs a new method for screening drugs for treating SAP according to the novel drug target.

The invention discloses a bacillus constitutive strong promoter and application thereof, and relates to the technical field of biology, and the nucleotide sequence of the promoter is SEQ ID NO: 2. The mRNA transcriptional level of the reporter gene is improved; the method is used for starting a target gene to efficiently express amylase in bacillus subtilis. The method is also used for starting a target gene to efficiently express alkaline protease in bacillus licheniformis. The novel constitutive promoter with enhanced transcriptional activity is developed by taking P43 as a starting template, and under the same host and culture conditions, the absolute level of the constitutive promoter for driving expression of a reporter gene or a target product is higher than that of the current P43 promoter, so that core power is provided for performance improvement of a bacillus cell factory.

The present invention relates to glucoamylase variants having improved thermostability and compositions comprising such variants. The present invention further relates to polynucleotides encoding such variants, vectors and host cells comprising genes encoding such variants, which may also enable the production of such variants. The present invention also relates to methods of liquefying starch-containing materials using or applying the variants or compositions, as well as the saccharification thus produced by the method. The present invention also relates to methods of saccharifying starch-containing materials using or applying the variants or compositions, as well as the saccharides thus produced by the method. The present invention further relates to processes for producing fermentation products from starch-containing or cellulosic-containing material, as well as an enzyme blend or composition, or a recombinant host cell or fermenting organism suitable for use in processes of the invention.

The application provides a promoter capable of improving the transcription level of a glycine nucleotide transcarbamylase gene and application, the promoter is a mutation based on the sequence shown in SEQ ID NO: 1, and two promoters capable of enhancing the transcription level of the glycine nucleotide transcarbamylase gene and capable of improving the expression of an alpha-amylase amyE gene from Bacillus subtilis subsp. subtilis str. 168 in coryneform bacteria are obtained.

The invention discloses high-purity resistant dextrin and a preparation method thereof, and the preparation method comprises the following steps: (1) adding 4, 3-alpha-glucosyltransferase into a maltodextrin aqueous solution or a starch solution treated by starch debranching enzyme to react, and after the reaction is finished, deactivating the enzyme; (2) adding alpha-amylase into a reactant in the step (1) to react, and deactivating the enzyme after the reaction is finished; (3) adding alpha-glucosidase into the reactant in the step (2) for reaction, and deactivating the enzyme after the reaction is finished; (4) adding saccharifying enzyme into the reactant in the step (3) to react, and deactivating the enzyme after the reaction is finished; and (5) adding yeast powder into the reactant in the step (4) for fermentation, filtering by a filter membrane after fermentation, collecting membrane filtrate, and drying to obtain the high-purity resistant dextrin. The preparation process is simple and efficient, the product purity is high, the proportion of alpha-1, 3 glucosidic bonds in the prepared resistant dextrin is 40% or above, and the purity of the resistant dextrin is 80-90%.

This invention belongs to the field of alcoholic beverage processing technology, specifically relating to a high-purity ginkgo wine and its preparation method. The preparation method comprises the following steps: ginkgo slurry undergoes preliminary enzymatic hydrolysis with neutral protease and amylase, followed by secondary enzymatic hydrolysis with pectinase and sodium bisulfite; brewing yeast fermentation broth is added for pre-fermentation; glutamine transaminase, *Saccharomyces cerevisiae* fermentation broth, and *Candida valerate* fermentation broth are added for post-fermentation; and finally, distillation and filtration are performed to obtain the ginkgo wine. This invention achieves complete decomposition and fermentation of ginkgo kernels through a two-step enzymatic hydrolysis and two-step fermentation process. The preparation method has advantages such as simple process and low cost, which is beneficial for industrial production; the prepared ginkgo wine has advantages such as clarity, strong aroma, high alcohol content, and low acidity.

Described are a recombinant host cell, a composition comprising a glucoamylase and methods of saccharifying the starch substrate using the glucoamylase. Moreover, the disclosure also relates to a process of producing fermentation products and a method for increasing starch digestibility in an animal as well as a method of producing a fermented beverage.

The present application relates to a kind of coix seed active ingredient extraction method and its steady-state microemulsion and its preparation method and application, the extraction method includes the following steps: b.coix seed coarse powder is added distilled water and mixed to form feed liquid, alpha-amylase is added, enzyme hydrolysis reaction is carried out, and extraction is carried out by ultrasonic treatment, and coix seed active ingredient extract is obtained;Wherein, the enzyme hydrolysis reaction temperature is 40~50 ℃.The present application aims to provide a kind of efficient, green, low-cost coix seed active ingredient extraction method, improves the extraction rate and retention rate of active ingredient, improves product stability, more suitable for industrial production.