354results about How to "Simple cultivation conditions" patented technology

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

Belonging to the field of environmental microorganism application, the invention discloses a Bacillus subtilis strain and application thereof. The strain is named as Bacillus subtilis CM-A12, is currently preserved in China Center For Type Culture Collection (called CCTCC for short) with a preservation number of CCTCC NO:M 2015688, and the preservation date is November 23, 2015. The Bacillus subtilis strain provided by the invention has the advantages of simple cultivation condition, high temperature resistance, acid and alkali resistance, and high salinity resistance, can secrete protease, amylase and cellulase, and can effectively improve water quality and reduce COD and ammonia nitrogen in water.

The invention relates to a genetically engineered bacterium producing tetrahydropyrimidine and a structuring method and application thereof. The genetically engineered bacterium is Escherichia coli with a specific genotype, comprises Halomonas-elogata-derived ectABC gene, has three gene deficiency variants including lysA, thrA and iclR and has corynebacterium glutamicum lysC gene controlled by a lac promoter and ppc gene controlled by a trc promoter. The defect that chemical synthesis methods have harsh reaction conditions and high energy consumption can be overcome by using the genetically engineered bacterium and utilizing glucose fermentation to produce tetrahydropyrimidine. The defect that Halomonas-elogata fermentation or enzyme-catalyzed methods are complex in process and high in production cost can be overcome. After fermentation for 20-28h, yield of tetrahydropyrimidine reaches 12-18g / L, and the genetically engineered bacterium has high industrial application value.

The invention discloses bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and an application thereof, and relates to the technical field of biological prevention and treatment of plant diseases, wherein the bacillus amyloliquefaciens B011 has the preservation number of CCTCC NO:M2014576; separation comprises the following steps: firstly, sampling; secondly, grinding, thirdly, centrifugation; fourth, purification, and fifthly, screening. The bacillus amyloliquefaciens B011 has the characteristics of relatively good antagonistic effect on tobacco bacterial wilt, good safety on the environment and easy industrialized production, not only can be used for preventing and treating tobacco bacterial wilt, but also can be used for preventing and treating P.parasitica, A.alternata keissler, also can be used for preventing and treating C.capsici, P.capsici, C.gloeosprioides, P.oryzae, C.cuc-umerinum and other pathogenic fungi.

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus and elliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The invention discloses a pine endosymbiotic bacterium which is classified and named after a new burkholderia cenocepacia NSM-05 and is stored in the China typical culture collection center with the storage number of CCTCC NO: M2011015 and the storage data of January 11, 2011. The invention also discloses application of the new burkholderia cenocepacia NSM-05 to the preventing and treating pine shoot dieback diseases and promoting the growth of pines. The invention also discloses an antagonistic inoculant for preventing and treating the pine shoot dieback diseases; the antagonistic inoculant is obtained by inoculating NSM-05 bacterium strains in a culture medium. The NSM-05 bacterium strain provided by the invention is the pine endosymbiotic bacterium, has strong bacteriostasis on the pine shoot dieback diseases, has the antagonism effect on multiple woods and crop pathogenic bacteria and road-spectrum antibacterial activity, is safe and non-toxic to people, livestock and plants and has good development and application prospects.

The invention discloses a bacillus amyloliquefacien and a preparation method and application thereof. According to the bacillus amyloliquefacien and the preparation method and the application thereof, the culture collection number of the bacillus amyloliquefaciens H6 is CCTCC No: M2013249; the bacillus amyloliquefaciens has obvious growth inhibition effect for gram-positive bacteria and gram-negative bacteria, strong degradation ability for starch, albumen, and cellulose, strong environment tolerance, and excellent effect for preventing diarrhea and promoting growing, and can not produce adverse effect with large doses of feeding; and the rear condition is simple, the cost is low, and the development and application prospect is excellent.

The invention discloses bacillus velezensis strain HN03 and application thereof. The bacillus velezensis strain HN03 has the accession number CCTCC M 2018084. The pure bacillus velezensis strain HN03has a good inhibitory effect on banana fusarium No. 1 and No. 4 physiological strains, and various other pathogenic bacteria.

The invention belongs to the field of biotechnology and particularly relates to Bacillus subtilis and application thereof. The Bacillus subtilis is Bacillus subtilis HS5B5. The strain is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6088. The Bacillus subtilis HS5B5 provided by the invention has a very strong restraining effect on the pathogen Cladosporiwm paeoniae of brown spot of peony, and can effectively prevent and cure brown spot of peony. The Bacillus subtilis HS5B5 is harmless to the ecological environment and cannot cause the resistance of the pathogen. The Bacillus subtilis HS5B5 is cultured under simple conditions, is easy to preserve, facilitates industrial production and has a favorable development and application prospect.

The invention discloses a Bacillus subtilis Y13 with the preservation number of CCTCC NO.M 208264, for effectively controlling oil tea anthrax, pertaining to the field of plant protection technology. At the same time, the invention discloses its separation method and applications, the Bacillus subtilis Y13 is obtained by flat plate facedown experiment screening, and can effectively inhibit the growth of oil tea anthrax bacteria, which has high proofing effect, good environment safety and favorable development and application prospects.

The invention discloses a bacillus pumilus JK-SX001 which is classified and named bacillus pumilus and collected in a China center for type culture collection (CCTCC) and has the collection number ofM 209026 on February 18th, 2009. The invention also discloses applications of the bacillus pumilus in preventing and treating dothiorella gregaria and promoting the growth of a poplar. The invention also provides a bactericide for preventing and treating the dothiorella gregaria, which is obtained by vaccinating the JK-SX001 in a culture medium to ferment and culture. The JK-SX001 is a plant endophyte, has obvious inhibiting effect on dothiorella gregaria, simple culture condition, easy storage and favorable development and application prospect and is easy for industrialized production.

The invention discloses a salt-tolerant bacteria T5S2 and applications in microbial fertilizer. A new specie salt-tolerant bacteria T5S2 can be separated from rhizosphere soil of Kalidium gracile in Sinkiang Junggar basin salt and alkali region, the salt-tolerant bacteria T5S2 can tolerate 1-15% NaCl, can grow well at 30 DEG C-37 DEG C, and can produce ACC deaminase and IAA indoleacetic acid without induction during growth, a microbial fertilizer prepared by utilizing the bacteria is applied to commercial crops of saline-alkaline land cotton, corns and the like, so that the germination rate of the saline-alkaline land cotton can achieve 77.8%, the survival rate can achieve 65.4%, the plant height, plant number and single boll plant number are obviously higher than cotton normally treated, the seedling rate of the saline-alkaline land corns can be increased to more than 80% from 64.5%, the production quantity of corn per mu is up to 600kg / mu, the total content of salt in soil of 0-30cm soil layer is reduced by 60-70%, and the microbial fertilizer has obvious salt reduction and production increase functions, thus having wide application values.

The invention relates to a protease-producing strain exiguobacterium sp. and applications thereof. A protease high-producing strain is screened out from deep sea muds through first screening with casein plates and second screening with shake flask fermentation, and identified as exiguobacterium sp. The protease-producing strain exiguobacterium sp. is collected in China General Microbiological Culture Collection Center on March 29, 2013. The collection number is CGMCC No. 7387. The protease-producing conditions are optimized by single factor experiments. The protease-producing strain exiguobacterium sp. is easy to culture, and is advantaged by simple culture conditions and stable hereditary properties.

The invention discloses a bacillus pumilus JK-SD001. Subclass thereof is named as Bacilluspumilus and is preserved in China Center for Type Culture Collection CCTCC, preservation number thereof is M 209095, and preservation date is May 4th, 2009. The invention also discloses application of bacillus pumilus for killing bursaphelenchus xylophilus with poison. The invention also provides a micro organism bursaphelenchus xylophilus killing agent, obtained by the way that JK-SD001 is inoculated to a culture medium to be fermented and cultured and filtering is carried out by virtue of a filter with the aperture of 0.22Mum. The micro organism bursaphelenchus xylophilus killing agent of the invention has higher toxicity on bursaphelenchus xylophilus (B. xylophilus), and culture condition is simple and preservation is easy, thus being easy for industrialized production and having good application prospect.

The invention discloses a biocontrol actinomycete strain for preventing and controlling tobacco bacterial wilt and application method thereof and belongs to the technical field of micro-organisms. The strain is streptomyces K4, is collected in China General Microbiological Culture Collection Center on March 24, 2014 and has the collection number of CGMCC No. 8949. The strain generates active substances to inhibit the growth of tobacco bacterial wilt and exhibits better prevention and control effects on the tobacco bacterial wilt. The biocontrol actinomycete strain is used for preparing a biocontrol microbial agent and the biocontrol microbial agent can be applied together with rooting water when tobacco seedlings are transplanted; or the biocontrol microbial agent is irrigated into the roots at 4-5 days after the tobacco seedlings are transplanted. The microbial agent can be applied as a bacterial fertilizer prepared by mixing the microbial agent and an organic fertilizer. The strain K4 is obtained from tobacco field rhizosphere soil and is ecologically and harmoniously compatible with soil so that the strain K4 has good application prospects in the biological control of tobacco bacterial wilt.

The invention relates to a Mucor circinelloides strain with high grease yield and application thereof. The invention discloses a Mucor circinelloides strain WJ11 and application thereof (a method for producing grease by using the strain). The method comprises the following steps: activating the strain to obtain an activated strain solution, carrying out seed culture under sterile conditions to obtain a seed culture solution, carrying out fermentation culture to obtain a fermentation culture solution, filtering, carrying out freeze-drying to constant weight to obtain a grease-containing Mucor circinelloides WJ11 dry strain, grinding, and extracting grease by a chloroform-methanol process. The Mucor circinelloides strain WJ11 has the advantages of high growth rate and high fatty acid content (up to 30-40% of biomass); the fatty acid content is 2-3 times of the control strain; and the biochemical analysis result shows that the Mucor circinelloides strain WJ11 has obviously higher enzyme activity related to fatty acid accumulation than the control group. The Mucor circinelloides strain has very wide application prospects when being used in microbial grease-producing industry and microbial grease accumulation analysis.

The invention provides a Vero cell line capable of stably expressing protein P54 of an African swine fever virus. The collection number of the cell line is CGMCC No.14316. The cell line not only can identify a polyclonal antibody of African swine fever, but also can generate specific reaction with positive serum of an African swine fever-infected pig. The monoclonal Vero cell line of an African swine fever P4 antigen can be used for stably expressing the protein P54 of the African swine fever virus, and can be used as a cell model or a virus infection model; the cell transmission stability ishigh, the growing speed is high, and culture conditions are simple; a large number of cell lines can be provided; the cell line has a good application prospect for researches on detection and pathogenesis mechanisms of the African swine fever.

The invention discloses a Bacillus licheniformis 201, which is preserved in China Center for Type Culture Collection that is located in Wuhan University, Logosun, Wuchang, Wuhan, Hubei, and with CCTCC NO. of M207092. The invention simultaneously discloses a preparation method and applications of the Bacillus licheniformis 201, which has strong bacteriostasis of banana wilt fusarium, wide bacteriostasis spectrum and antagonism to various pathogenic fungi including banana wilt fusarium, watermelon wilt fusarium, tomato wilt fusarium, banana colletotrichum, gloeocystidium colletotrichum, corn leaf spot curvularia, cotton verticillium lecanii, gibberella zeae, and the like, and the Bacillus licheniformis 201 has high control efficiency, good environmental security and good development and application prospect.

The invention discloses a temporary immersion type culture method and culture device for dendrobe. Rooted sterile dendrobe seedlings are transferred to a temporary immersion type culture device and are subjected to liquid culture under a natural condition, no sugars are added into culture solution, no carbon dioxide needs to be introduced, and carbon sources required for the growth of the dendrobe are from self photosynthesis. According to the method, a dendrobe culture condition is simplified, and a plantation cost is lowered.

The invention discloses a biotechnology for petroleum desulfurization and denitrification and an application. A mixed microbial agent is prepared from activated geobacillus thermoglucosidasius w-1, mycobacterium goodiii, thermotoga neapolitana and small thermophilic and oil-degraded bacilli according to the volume ratio of 1:1:1:1. The mixed microbial agent reacts with high-sulfur diesel oil and inferior oil slurry in an organic salt solution, and the sulfur content and the nitrogen content of the two kinds of oil before and after the reaction are measured. A production method is simple in process, needed equipment is simple, cost is low, environment friendliness is achieved, produced fuel oil is good in quality, an ideal treatment effect is achieved for the oil, thermophilic microorganisms achieving the simultaneous desulfurization and denitrification function are adopted for achieving simultaneous desulfurization and denitrification of petroleum in the high-temperature environment, the biological desulfurization and denitrification efficiency of fuel oil can be remarkably improved, the environment pollution problems generated after use can be effectively solved, excellent characteristics of industrially-applied bacterial strains are achieved, and the bacterial strains have wide industrial application potential.

The invention discloses a method of culturing dendrobium officinale plantlets and belongs to the field of plant tissue culturing technology. The method comprises the steps: step 1, induction and culturing of seeds: choosing dendrobium officinale fruit pods as explants, spreading seeds in the fruit pods on an explant induction culture medium and inducing seeds to germinate to form sprouts; step 2, multiplication and subculture of sprouts: grafting the sprouts on a multiplication and subculture medium, placing the multiplication and subculture medium in a culturing room and culturing to obtain dendrobium officinale seedlings; step 3, culturing of rooting seedlings: grafting the dendrobium officinale seedlings on a rooting culture medium and culturing to obtain strong dendrobium officinale seedlings; and step 4, transplanting: hardening the strong dendrobium officinale seedlings subjected to the rooting culturing and then transplanting the strong seedlings. The method of culturing dendrobium officinale plantlets solves problems of slow rooting and lower survival rate in the traditional culturing method of dendrobium officinale and provides convenience to the large-scale culturing of high-quality dendrobium officinale plantlets.

The invention discloses bacillus subtilis. The bacillus subtilis with the preservation number of CGMCC No.7542 is preserved in the China General Microbiological Culture Collection Center on April 28, 2013. The invention further provides an application of the bacillus subtilis in strawberry root rot. The bacillus subtilis B85 has a very good inhibition effect on strawberry root rot, grey mould fruit rot of strawberry, strawberry anthracnose and other pathogenic fungi, and also has a very good preventing and controlling effect on other plant diseases. The bacillus subtilis has a high proliferation speed, can be artificially cultured, is easy to operate, facilitates production and application, has the advantages of strong stress resistance and the like, especially has an important meaning to prevention and control of fungi caused strawberry root rot, is simple in culture condition and easy to preserve, is convenient for industrial production, and has excellent development and application prospects.

The invention discloses a banana endophytic bacillus velezensis strain and an application thereof. The name of the bacillus velezensis strain is bacillus velezensis BS (Bacillus velezensis). The preservation number is GDMCC No: 60930, and the strain is preserved in Guangdong Province Microbial Culture Collection Center of 59 # building of 100 # courtyard of Xianlie Middle Road of Guangzhou City onDecember 11, 2019. The strain disclosed by the invention belongs to banana endophytic bacteria, has no pathogenicity and has a growth promoting effect on bananas. The bacterial strain and the biological agent thereof have a remarkable inhibition effect on banana wilt pathogen FOC4, the banana wilt symptom is obviously relieved, and the biological agent is environment-friendly and non-toxic in source and has small influence on the ecological environment; the strain has different degrees of inhibition effects on plant pathogenic fungi of different genera; the culture conditions are simple, a propagation speed is fast, thalli and a bacterial liquid can serve as main bodies for prevention and control, storage and transportation are easy, and good development and application prospects are achieved.

The invention discloses streptomyces and an application thereof. A preservation number of the streptomyces is CGMCC No.9034. The streptomyces is endophyte, has strong inhibiting effect to various plant pathogenic fungi, has many advantages in plant disease biological control, such as numerous amount, massive existence in and out of bodies of plants, diversification in action mode to pathogenic bacteria, rapid speed in propagation, strong root-colonizing capacity in and out of the bodies of the plants, promotion in the growth of the plants and the like and particularly has important significance of preventing and treating soybean sclerotinia sclerotiorum and poplar canker. Meanwhile, the streptomyces is simple in culture condition, is easy to preserve, is easy in industrial production and has good development and application prospect.

The invention provides Trichoderma asperellum with the antagonistic action on banana wilt and application of the Trichoderma asperellum. The Trichoderma asperellum with the antagonistic action on banana wilt is Trichoderma asperellum PZ6 which is preserved in the China Center for Type Culture Collection on January 18th, 2016 with the preservation serial number of CCTCC No: M 2016043 at the address of Wuhan University in Wuhan, China. As separated from plant rhizosphere soil, the Trichoderma asperellum PZ6 is a soil inhabitant and is high in rhizosphere colonization capability, good in control effect and especially high in antagonistic action on banana wilt pathogenic bacteria; besides, the culture conditions are simple, preservation and industrial production are easy, and good development and application prospects are achieved.

The invention discloses bacillus amyloliquefaciens subsp.plantarum which is classified and named as bacillus amyloliquefaciens subsp.plantarum B232 and preserved in the China General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collection of Microorganisms (CCCCM) on June 9th, 2014, and has the preservation number of CGMCCNo.9279. The bacillus amyloliquefaciens subsp.plantarum B232 is mainly used for carrying out biological prevention and treatment on Dothichiza populea Sacc.et Briard, and has good functions of inhibiting Dothichiza populea Sacc.et Briard germs, dendrobium officinale botrytis and other various pathogenic fungi. The bacillus amyloliquefaciens subsp.plantarum B232 taken as an antagonistic bacterium also has good effects of preventing and treating other plant diseases. The bacillus amyloliquefaciens subsp.plantarum B232 has the advantages of being rapid in propagation speed, easy to operate, convenient in production application, strong in stress resistance and the like, and can be artificially cultured. The bacillus amyloliquefaciens subsp.plantarum B232 requires a simple culture condition and is easy to store and favorable for industrial production. Thus, the bacillus amyloliquefaciens subsp.plantarum B232 has a good development and application prospect.

The invention discloses umbelopsis dimorpha. The umbelopsis dimorpha belongs to umbelopsis genus of mucorale order of zygomycete subdivision; the strain number of the umbelopsis dimorpha is JSNL001-2, the umbelopsis dimorpha is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC No.9546, and the preservation date is August 15th, 2014. The umbelopsis dimorpha JSNL001-2 has remarkable effects in increasing the growth speed and improving the drought resistance of dendrobium officinale, can be cultured artificially, is high in reproduction speed, is convenient in on-scale production and has good development and application prospects.