353results about How to "Simple cultivation conditions" patented technology

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

The invention relates to a genetically engineered bacterium producing tetrahydropyrimidine and a structuring method and application thereof. The genetically engineered bacterium is Escherichia coli with a specific genotype, comprises Halomonas-elogata-derived ectABC gene, has three gene deficiency variants including lysA, thrA and iclR and has corynebacterium glutamicum lysC gene controlled by a lac promoter and ppc gene controlled by a trc promoter. The defect that chemical synthesis methods have harsh reaction conditions and high energy consumption can be overcome by using the genetically engineered bacterium and utilizing glucose fermentation to produce tetrahydropyrimidine. The defect that Halomonas-elogata fermentation or enzyme-catalyzed methods are complex in process and high in production cost can be overcome. After fermentation for 20-28h, yield of tetrahydropyrimidine reaches 12-18g/L, and the genetically engineered bacterium has high industrial application value.

The invention discloses bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and an application thereof, and relates to the technical field of biological prevention and treatment of plant diseases, wherein the bacillus amyloliquefaciens B011 has the preservation number of CCTCC NO:M2014576; separation comprises the following steps: firstly, sampling; secondly, grinding, thirdly, centrifugation; fourth, purification, and fifthly, screening. The bacillus amyloliquefaciens B011 has the characteristics of relatively good antagonistic effect on tobacco bacterial wilt, good safety on the environment and easy industrialized production, not only can be used for preventing and treating tobacco bacterial wilt, but also can be used for preventing and treating P.parasitica, A.alternata keissler, also can be used for preventing and treating C.capsici, P.capsici, C.gloeosprioides, P.oryzae, C.cuc-umerinum and other pathogenic fungi.

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

The invention belongs to the field of biotechnology and particularly relates to Bacillus subtilis and application thereof. The Bacillus subtilis is Bacillus subtilis HS5B5. The strain is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6088. The Bacillus subtilis HS5B5 provided by the invention has a very strong restraining effect on the pathogen Cladosporiwm paeoniae of brown spot of peony, and can effectively prevent and cure brown spot of peony. The Bacillus subtilis HS5B5 is harmless to the ecological environment and cannot cause the resistance of the pathogen. The Bacillus subtilis HS5B5 is cultured under simple conditions, is easy to preserve, facilitates industrial production and has a favorable development and application prospect.

The invention discloses a Bacillus subtilis Y13 with the preservation number of CCTCC NO.M 208264, for effectively controlling oil tea anthrax, pertaining to the field of plant protection technology. At the same time, the invention discloses its separation method and applications, the Bacillus subtilis Y13 is obtained by flat plate facedown experiment screening, and can effectively inhibit the growth of oil tea anthrax bacteria, which has high proofing effect, good environment safety and favorable development and application prospects.

The invention discloses a bacillus pumilus JK-SX001 which is classified and named bacillus pumilus and collected in a China center for type culture collection (CCTCC) and has the collection number ofM 209026 on February 18th, 2009. The invention also discloses applications of the bacillus pumilus in preventing and treating dothiorella gregaria and promoting the growth of a poplar. The invention also provides a bactericide for preventing and treating the dothiorella gregaria, which is obtained by vaccinating the JK-SX001 in a culture medium to ferment and culture. The JK-SX001 is a plant endophyte, has obvious inhibiting effect on dothiorella gregaria, simple culture condition, easy storage and favorable development and application prospect and is easy for industrialized production.

The invention discloses a salt-tolerant bacteria T5S2 and applications in microbial fertilizer. A new specie salt-tolerant bacteria T5S2 can be separated from rhizosphere soil of Kalidium gracile in Sinkiang Junggar basin salt and alkali region, the salt-tolerant bacteria T5S2 can tolerate 1-15% NaCl, can grow well at 30 DEG C-37 DEG C, and can produce ACC deaminase and IAA indoleacetic acid without induction during growth, a microbial fertilizer prepared by utilizing the bacteria is applied to commercial crops of saline-alkaline land cotton, corns and the like, so that the germination rate of the saline-alkaline land cotton can achieve 77.8%, the survival rate can achieve 65.4%, the plant height, plant number and single boll plant number are obviously higher than cotton normally treated, the seedling rate of the saline-alkaline land corns can be increased to more than 80% from 64.5%, the production quantity of corn per mu is up to 600kg/mu, the total content of salt in soil of 0-30cm soil layer is reduced by 60-70%, and the microbial fertilizer has obvious salt reduction and production increase functions, thus having wide application values.

The invention relates to a protease-producing strain exiguobacterium sp. and applications thereof. A protease high-producing strain is screened out from deep sea muds through first screening with casein plates and second screening with shake flask fermentation, and identified as exiguobacterium sp. The protease-producing strain exiguobacterium sp. is collected in China General Microbiological Culture Collection Center on March 29, 2013. The collection number is CGMCC No. 7387. The protease-producing conditions are optimized by single factor experiments. The protease-producing strain exiguobacterium sp. is easy to culture, and is advantaged by simple culture conditions and stable hereditary properties.

The invention discloses a bacillus pumilus JK-SD001. Subclass thereof is named as Bacilluspumilus and is preserved in China Center for Type Culture Collection CCTCC, preservation number thereof is M 209095, and preservation date is May 4th, 2009. The invention also discloses application of bacillus pumilus for killing bursaphelenchus xylophilus with poison. The invention also provides a micro organism bursaphelenchus xylophilus killing agent, obtained by the way that JK-SD001 is inoculated to a culture medium to be fermented and cultured and filtering is carried out by virtue of a filter with the aperture of 0.22Mum. The micro organism bursaphelenchus xylophilus killing agent of the invention has higher toxicity on bursaphelenchus xylophilus (B. xylophilus), and culture condition is simple and preservation is easy, thus being easy for industrialized production and having good application prospect.

The invention discloses a temporary immersion type culture method and culture device for dendrobe. Rooted sterile dendrobe seedlings are transferred to a temporary immersion type culture device and are subjected to liquid culture under a natural condition, no sugars are added into culture solution, no carbon dioxide needs to be introduced, and carbon sources required for the growth of the dendrobe are from self photosynthesis. According to the method, a dendrobe culture condition is simplified, and a plantation cost is lowered.

The invention discloses a biotechnology for petroleum desulfurization and denitrification and an application. A mixed microbial agent is prepared from activated geobacillus thermoglucosidasius w-1, mycobacterium goodiii, thermotoga neapolitana and small thermophilic and oil-degraded bacilli according to the volume ratio of 1:1:1:1. The mixed microbial agent reacts with high-sulfur diesel oil and inferior oil slurry in an organic salt solution, and the sulfur content and the nitrogen content of the two kinds of oil before and after the reaction are measured. A production method is simple in process, needed equipment is simple, cost is low, environment friendliness is achieved, produced fuel oil is good in quality, an ideal treatment effect is achieved for the oil, thermophilic microorganisms achieving the simultaneous desulfurization and denitrification function are adopted for achieving simultaneous desulfurization and denitrification of petroleum in the high-temperature environment, the biological desulfurization and denitrification efficiency of fuel oil can be remarkably improved, the environment pollution problems generated after use can be effectively solved, excellent characteristics of industrially-applied bacterial strains are achieved, and the bacterial strains have wide industrial application potential.

The invention discloses a banana endophytic bacillus velezensis strain and an application thereof. The name of the bacillus velezensis strain is bacillus velezensis BS (Bacillus velezensis). The preservation number is GDMCC No: 60930, and the strain is preserved in Guangdong Province Microbial Culture Collection Center of 59 # building of 100 # courtyard of Xianlie Middle Road of Guangzhou City onDecember 11, 2019. The strain disclosed by the invention belongs to banana endophytic bacteria, has no pathogenicity and has a growth promoting effect on bananas. The bacterial strain and the biological agent thereof have a remarkable inhibition effect on banana wilt pathogen FOC4, the banana wilt symptom is obviously relieved, and the biological agent is environment-friendly and non-toxic in source and has small influence on the ecological environment; the strain has different degrees of inhibition effects on plant pathogenic fungi of different genera; the culture conditions are simple, a propagation speed is fast, thalli and a bacterial liquid can serve as main bodies for prevention and control, storage and transportation are easy, and good development and application prospects are achieved.

The invention discloses streptomyces and an application thereof. A preservation number of the streptomyces is CGMCC No.9034. The streptomyces is endophyte, has strong inhibiting effect to various plant pathogenic fungi, has many advantages in plant disease biological control, such as numerous amount, massive existence in and out of bodies of plants, diversification in action mode to pathogenic bacteria, rapid speed in propagation, strong root-colonizing capacity in and out of the bodies of the plants, promotion in the growth of the plants and the like and particularly has important significance of preventing and treating soybean sclerotinia sclerotiorum and poplar canker. Meanwhile, the streptomyces is simple in culture condition, is easy to preserve, is easy in industrial production and has good development and application prospect.

The invention relates to an L- lactate dehydrogenase gene, and the nucleotide sequence represents as SEQ ID NO. 1. The invention further provides an expression vector with the gene, a recombinant Escherichia coli, a recombinant Escherichia coli construction method and the application thereof during phenylpyruvic acid conversion and (S)-2-hydroxy-3-phenylpropionic acid synthesis. The method includes after performing codon optimization on (Bacillus megaterium Z2013513) CCTCC NO. M2013244 L- lactate dehydrogenase gene, constructing the expression vector, inducing expression of the Escherichia coli, and obtaining the L-PLA production strain with simple culture condition, convenience for usage and wide application. The yield for the recombinant Escherichia coli converting the L-PLA in whole-cell and single-batch manners is 27.13mmol / L, the converting rate is 77.5%, and the basis is provided for increasing the L-PLA yield and producing rate for subsequent fermentor continuous feeding.

The invention discloses bacillus amyloliquefaciens subsp.plantarum which is classified and named as bacillus amyloliquefaciens subsp.plantarum B232 and preserved in the China General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collection of Microorganisms (CCCCM) on June 9th, 2014, and has the preservation number of CGMCCNo.9279. The bacillus amyloliquefaciens subsp.plantarum B232 is mainly used for carrying out biological prevention and treatment on Dothichiza populea Sacc.et Briard, and has good functions of inhibiting Dothichiza populea Sacc.et Briard germs, dendrobium officinale botrytis and other various pathogenic fungi. The bacillus amyloliquefaciens subsp.plantarum B232 taken as an antagonistic bacterium also has good effects of preventing and treating other plant diseases. The bacillus amyloliquefaciens subsp.plantarum B232 has the advantages of being rapid in propagation speed, easy to operate, convenient in production application, strong in stress resistance and the like, and can be artificially cultured. The bacillus amyloliquefaciens subsp.plantarum B232 requires a simple culture condition and is easy to store and favorable for industrial production. Thus, the bacillus amyloliquefaciens subsp.plantarum B232 has a good development and application prospect.