943results about How to "Short fermentation cycle" patented technology

The invention relates to a bio-protein feed prepared by adopting a cellulose raw material, and the bio-protein feed provided by the invention comprises an organic material and complex bacteria, wherein the weight of the complex bacteria is 1-10% of that of the organic material; and the organic material comprises the cellulose raw material, rapeseed meal and bran; the cellulose raw material is one or more of corn stalks, corn cobs, straw, manioc waste and fruit residue; and the complex bacteria comprises bacillus subtilis, microzyme, lactobacillus and cellulose decomposition bacteria, and the cellulose decomposition bacteria is one or more of trichoderma harzianum and white-rot fungi. In the bio-protein feed, the content of probiotics is more than 108cfu/g, the bio-protein feed contains a variety of prolease, cellulase, amylase and the like, the preparation process is simple, and the fermentation period is short. The invention further discloses a preparation method of the feed.

The invention provides a method for preparing biological flocculant by utilizing Bacillus licheniformis, which relates to a biological flocculant. The invention provides a method for preparing biological flocculant by utilizing Bacillus licheniformis, which has high flocculation activity, low cost for raw materials and great industrial application potential. A microorganism is Bacillus licheniformis. The method comprises the following steps that: lawn on a fresh inclined plane is transferred to a seed culture medium for culture and then transferred to a fermentation culture medium for culture so as to obtain biological-flocculant fermentation broth; the biological-flocculant fermentation broth is centrifuged to remove precipitate and collect supernatant; the supernatant is added with ethanol, kept to stand, centrifuged and then removed; the precipitate is added with absolute ethanol, stirred and centrifuged to remove the supernatant; ethanol precipitate is added with hexadecyl trimethyl ammonium bromide and centrifuged to remove the supernatant so as to obtain the precipitate; the precipitate is dissolved in a NaCl solution, added with the absolute ethanol, stirred, stood and centrifuged to remove the supernatant so as to obtain the precipitate; and the precipitate is frozen and dried in vacuum so as to obtain pure biological flocculant.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g/l.

The invention discloses a five-grain fen-flavor liquor and a preparation method thereof. The five-grain fen-flavor liquor is prepared through diastatic fermentation of brewing raw materials consisting of sorghum, rice, glutinous rice, wheat and corn by using Qingcha Daqu. The preparation method for the liquor comprises the following steps: preparing the Qingcha Daqu; mixing the brewing raw materials with the Qingcha Daqu; carrying out diastatic fermentation; and carrying out distillation. The liquor provided by the invention has pure, mild and mellow taste and satisfies current requirements for taste and health care by drinkers; the flavor of sorghum, sweetness of corn, pureness of rice, sweetness of glutinous rice and unique fragrance generated by fermentation of wheat collagen and glutenin in wheat are combined in the liquor, and deficiency in brewing with only a grain is substantially improved; and the liquor has no special odor, can be used as a base wine for medicinal liquor and functional liquor, is applicable as a flavoring liquor for development of a high-grade liquor and has a good application prospect.

The invention relates to a genetically engineered bacterium producing tetrahydropyrimidine and a structuring method and application thereof. The genetically engineered bacterium is Escherichia coli with a specific genotype, comprises Halomonas-elogata-derived ectABC gene, has three gene deficiency variants including lysA, thrA and iclR and has corynebacterium glutamicum lysC gene controlled by a lac promoter and ppc gene controlled by a trc promoter. The defect that chemical synthesis methods have harsh reaction conditions and high energy consumption can be overcome by using the genetically engineered bacterium and utilizing glucose fermentation to produce tetrahydropyrimidine. The defect that Halomonas-elogata fermentation or enzyme-catalyzed methods are complex in process and high in production cost can be overcome. After fermentation for 20-28h, yield of tetrahydropyrimidine reaches 12-18g/L, and the genetically engineered bacterium has high industrial application value.

A method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 belongs to the technical field of microorganisms. In the invention, Bacillus coagulans CGMCC No.2602 is adopted, and under the condition of no oxygen supply, starchiness hydrolyzed sugar or dextrose is fermented by a semicontinuous intermittent fermentation way or an inter-sugar-compensating fermentation way to generate L-lactic acid with high optical purity. The invention has the advantages that the gemma property of the Bacillus coagulans is stable, and the L-lactic acid obtained by inoculating and fermenting the starchiness hydrolyzed sugar has high optical purity and rate of conversion of sugar and acid and short fermentation period; in addition, the semicontinuous intermittent fermentation way saves the time for preparing seeds by a continuous reladling and subculturing method, shortens the fermentation period, enhances the fermentation strength and obtains the L-lactic acid with both relatively high rate of conversion of sugar and acid and purity.

The invention discloses a multifunctional high efficient organic material decomposition agent and a preparation method thereof. The decomposition agent of the invention comprises composite original strains consisting of bacillus amyloliquefaciens, Aspergillus oryzae, Trichoderma viride, and Saccharomyces cerevisiae and an organic material carrier, and an inorganic carrier. Strains of the decomposition agent can be mutualistic, and have complementary advantages. The adaptation is wide, and a plurality of organic matter clastic enzymes can be produced by metabolism. The decomposition agent has high activity and can be used for fermentation and conversion of a plurality of organic materials such as livestock and poultry excrements, crop straws, city sludge, food waste, food processing and pharmaceutical waste residues, and has fast fermentation start, fast deodorization, low temperature resistance, and low moisture resistance. The fermentation period is shorter by 5-7 days than that in the same technology. Fermented and converted organic fertilizers have the obvious rhizosphere ecological regulation and control function, promote the root growth, and improve the root vitality. A plurality of soil borne diseases are prevented by improving the flora diversity, hyperparasitism and biological antagonism.

The invention discloses pig-chicken excrement fermenting compound bacteria, a preparation method and a method of utilizing the compound bacteria to ferment and prepare an organic fertilizer. The fermenting compound bacteria comprise the following raw material bacteria: bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus megatherium and trichoderma viride. The method for preparing the organic fertilizer comprises the following steps: mixing pig-chicken excrement with crushed straws, adding the fermenting compound bacteria to the mixture according to a dosage of 1-2 Kg per ton, mixing uniformly, the moisture being 50-65 wt%, stacking the mixed raw materials, keeping the temperature at 55-65 DEG C to conduct fermentation, two days after the fermentation, turning the stacked materials for the first time, and then turning the materials once every one or two days, keeping the moisture at 50-65 wt% during the fermentation phase, and after fermentation, obtaining the organic fertilizer with a moisture content of 20-30 wt%. The pig-chicken excrement fermenting compound bacteria disclosed by the invention can be used for fermenting the pig and chicken excrement; compared to the prior art, the method for preparing the organic fertilizer is low in productive investment and production cost, convenient to use, free of odor, and good in the fermentation and decomposing effect.

The invention discloses a moisturizing eye mask prepared from bacterial cellulose. The method for preparing a moisturizing eye mask from bacterial cellulose comprises: inoculating 1-2 rings of activated inclined seed Gluconacetobacter xylinum into a medium, and conducting oscillating culture to obtain a seed solution; then inoculating the seed solution into the medium, fully oscillating the bacterial solution obtained by mixing the seed solution and the medium to make them mixed uniformly, then conducting stationary culture, thus generating a bacterial cellulose membrane floating on the solution surface, flushing the generated bacterial cellulose membrane with water to remove the medium and impurities on the membrane surface, then immersing the membrane in an alkali solution to remove thalli and residual medium from the membrane, and finally flushing the membrane with distilled water till the pH value of the membrane is measured up to 7-7.2, thus obtaining a bacterial cellulose wet membrane, which is then cut into an eye mask. The eye mask has the advantages of good moisturizing property and biological tissue compatibility, no toxicity, harmlessness, and simple material processing technology.

The invention relates to the field of biological engineering. The culture medium formula of selenium-enriched edible fungi comprises the following components in percentage by weight: 0.4 to 0.6 percent of bean straw chopper, 0.15 to 0.25 percent of leaf powder, 0.05 to 0.1 percent of magnesium sulfate, 0.2 to 0.4 percent of corn flour, 0.2 to 0.5 percent of selenium-enriched rice bran, and 0.1 to0.3 percent of ginkgo leaf powder. The selenium-enriched edible fungi producing method comprises the following steps: preparing a culture medium; regulating; sterilizing; inoculating and culturing; and accelerating mushrooms, wherein, during liquid strain culture, a sodium selenite component is added in a culture solution; and a strain is cultured in the culture solution for culturing the strain.The cultured selenium-enriched edible fungi have high content of selenium, can be used for effectively improving the selenium level in blood of a human body and improving the disease resistance of the human body, and have excellent disease treatment and body-building efficacies.

The invention discloses bacillus subtilis. The bacillus subtilis which is classified and named as G-34(Bacillus subtilis G-34) is collected in China Center for Type Culture Collection on January 6, 2014, and the collection register number is CCTCC NO:M2014003. A bacillus subtilis strain is obtained via mutation breeding on Bacillus subtilis strain separated from polluted soil of Shengli Oil Field; a culture medium with glycerin as the only carbon source can be rapidly utilized for preparing a lipopeptide biosurfactant surfactin at a high yield. With crude glycerin as a substrate, the pure surfactin can be obtained through the steps of bacillus subtilis fermenting-transforming, centrifugal degerming, acid precipitating, freeze-drying, and methanol extracting.

The invention discloses a preparation method and an application method of a seaweed industrial solid waste organic fertilizer. The preparation method comprises the steps of: mixing seaweed solid waste with additives, adding water, and adding a decomposition accelerator to perform micro-anaerobic fermentation, drying and crushing after the fermentation procedure to obtain the seaweed industrial solid waste organic fertilizer. The application method of the seaweed industrial solid waste organic fertilizer comprises the step of directly applying or adding other inorganic fertilizers to prepare an organic-inorganic compound fertilizer. The preparation method of the seaweed industrial solid waste organic fertilizer, disclosed by the invention, has the advantages of short fermentation period, simple fermentation technique and high compost nutrient storage rate.

The invention provides a method for preparing fermented tea with combination of artificial inoculation and liquid state fermentation process. The method comprises the following steps of: firstly, crushing large fresh tea leaves or sunned primary tea from Yunnan, extracting the tea by water, and filtering the extract to obtain a water extract or adding water to the extract to obtain a tea and water mixed solution; secondly, adding strains into the water extract or the mixed solution for liquid state fermentation at 30-60 DEG C for 5 to 14 days; and thirdly, filtering after the fermentation to obtain fermentation liquor, inactivating, centrifuging or filtering the fermentation liquor to obtain fermented tea juice, and concentrating and drying the fermented tea juice according to requirements to obtain the tea powder.

The invention relates to a fermenting method for producing pullulanuse polysaccharide, which is characterized in that the method comprises following steps: firstly, preparing seed culture medium, secondly, preparing fermenting initial culture medium, thirdly, inoculating liquid seeds into the fermenting initial culture medium, wherein the inoculation amount is 3-8%, the fermentation stirring speed is 200-500rpm, the fermentation temperature is 29 DEG C +- 1 DEG C, the ventilation volume is 0.5-10V/V, and the bed pressure is 0.01-0.02MPa, fourthly, complementarily adding the other carbon source after fermenting for 24 hours, wherein the adding amount is 40-80g/L and the adding liuqid is sucrose solution or glucose solution or starch hydrolysate whose pH value is 40-60, and continuingly fermenting for 60-72 hours after adding the carbon source, fifthly, flocculating, hyper-filtrating, barrier-separating, concentrating and drying the fermenting liquid and obtaining the pullulanuse polysaccharide whose molecular weight is between 20-60 million daltons. The invention has simple technique and significant effects and prominently shortens the fermenting time, the fermenting time is 80-100 hours, the production efficiency is increased greatly, energy is saved, the yield rate of product is greatly improved, and the yield can reach by 50%.

The invention discloses a method ganoderma fermentation method which includes carrying out the deep liquid fermentation of incline culturing, top-grade seed culturing and fermentation culturing on submerged bacterial strain in turn. The method also provides a purple chi mycelium manufactured by the fermentation method. In the invention, the polysaccharide content of intracellular polysaccharide extracted in the obtained ganoderma mycelium can achieve 39 to 75 percent through the improvement on the parameters and the culturing medium of the deep fermentation technique of the purple chi de; besides, the purple chi mycelium has higher anti-tumor activity.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

The invention relates to a biological drying method for treating urban domestic dewatered sludge, which belongs to the technical field of environmental protection. The method comprises the following steps of: mixing the dewatered sludge with crushed auxiliary materials and feeding the mixture into a fermentation cabin; adding fresh composted sludge from the top of the cabin, enclosing the fermentation cabin for a fermentation reaction, mixing a zymophyte agent special for sludge with the fresh composted sludge and scattering the mixture into the fermentation cabin; uniformly stirring, uniformly ventilating and aerating from the bottom of the cabin, and exhausting air from the top of the fermentation cabin until the central temperature of a compost is between 55 and 65 DEG C; and ventilating from the bottom of the fermentation cabin and inducing air from the top of the fermentation cabin to keep the central temperature of the compost in the fermentation cabin for 3 to 6 days, stirring the compost, maintaining for 2 to 4 days until the water content of the compost is reduced, continually ventilating from the bottom of the fermentation cabin and inducing air from the top of the fermentation cabin, stirring until the water content of the compost is less than 40 percent, and discharging. The dewatered sludge prepared by the method is a harmless and scentless product with a stable property, and can be used as fertilizers, covering soil and the like in agriculture and forestry and landfill yards.

The invention discloses a genetically engineered bacterium for producing L-arginine and a construction method and application thereof. According to the invention, a gene for encoding carbamyl phosphate synthetase and a gene for encoding L-arginine biosynthetic pathway enzyme are integrated in escherichia coli; a synthetic route of arginine in escherichia coli and metabolic flux related to argininein a whole amino acid metabolic network are analyzed and reconstructed to obtain genetically engineered bacteria which are clear in genetic background, do not carry plasmids, are not mutated and canstably and efficiently produce L-arginine; and the genetically engineered bacteria have good L-arginine production capacity.

The present invention discloses coupled deep and shallow layer static fermentation process of producing high fiber coconut fruit. The process includes inoculating pyracetic bacillus to the fermenting culture medium for deep layer static fermentation; and subpacking fermented liquid with wet bacillus concentration of 2.0-3.0 g/l in sterilized shallow containers for shallow layer static fermentation, so as to obtain high fiber coconut fruit with gel film in the required thickness. The production process has shortened shallow layer fermentation period, high production efficiency and less hetero bacterial contamination, and is suitable for industrial production.

The invention discloses a method for manufacturing flavor fermented blank bean, belonging to the field of food processing technology. The method has the process flow of screening raw materials, wetting by water, cooking, inoculating, making starter, selecting the starter, blending, canning, sunning dew and obtaining a finished product. The method has the beneficial effects that the fermentation period is shortened to one or two month(s) from the original more than one year; and the prepared fermented blank bean is rich in flavor, crimson in color and delicious in taste.

The invention relates to a bacillus amyloliquefaciens plant subspecies ZFH-3 microbial agent and application thereof and belongs to the field of plant protection and disease prevention and treatment. The microbial agent per g contains 2*10<10>-1.2*10<11>cuf bacillus amyloliquefaciens plant subspecies ZFH-3 living spores, and the ZFH-3 strain is classified and named Bacillus amyloliquefaciens subsp.Plantarum, wherein the collection number is CGMCC No. 9884. The agent has a remarkable inhibiting effect on various pathogens of crops, shows a relatively good preventing and treating effect on prevention and treatment of soil-borne diseases and can effectively prevent and treat soil-borne diseases of vegetables, so that the loss is damaged and the field efficacy is over 59%. The microbial agent is non-toxic and safe to human bodies, replaces chemical agents for preventing and treating soil-borne diseases, conditioning soil and promoting plant growth, and has a good application prospect.

The invention discloses a soilless plant cultivation substrate and a preparation method thereof. The preparation method of the cultivation substrate disclosed by the invention comprises the following steps: firstly, uniformly mixing agricultural and forestry waste, water, a fermentation catalyst, a fermentation fungicide, bamboo vinegar and/or pyroligneous liquor to carry out fermentation treatment for the first time to prepare a first fermentation substrate; and then, adding the fermentation catalyst, the water, the bamboo vinegar and/or the pyroligneous liquor into the first fermentation substrate to carry out fermentation treatment for the second time to obtain the soilless plant cultivation substrate, wherein the fermentation catalyst comprises rhamnolipid. The plant cultivation substrate prepared by the preparation method disclosed by the invention is balanced in nutrition, high in nutritional ingredient content, reasonable in structure, stable in physical property and convenient for being absorbed by plants, satisfies plant growth needs, improves survival rate for plant cultivation and ornamental effect.

The invention provides a black skin termitomyces albuminosus mycelium culture method which includes the steps: selecting and activating black skin termitomyces albuminosus mother strains, inoculating the strains on an inclined medium and enlarging culture until mycelia overgrow an inclined surface by more than 2/3; selecting strains with fine mycelium growth, inoculating the strains in a liquid medium and placing the liquid medium in a swing bed with the temperature of 23-24 DEG C to perform constant temperature culture for 3-5 days; throwing the strains cultured in the second step into a fermentation tank for culture to obtain mycelia by filtering, and drying the mycelia in a drying oven with the temperature of 65 DEG C to reach constant weight. The fermentation temperature is 22-25 DEG C, the rotating speed is 120-125rpm/min, and culture time is 72-96h. Fermentation cycle is short, mycelium yield is high, pollutants, waste and the like in the production process are avoided, organic germanium content is high, and germanium can be supplemented.