19results about How to "Short fermentation cycle" patented technology

The invention discloses a tryptophanase mutant and application thereof in production of indole, and relates to the technical field of microbial engineering. Through random mutation and site screening, amino acid replacement is carried out at the 389th site of SEQ ID NO: 1 to obtain a new tryptophan enzyme, and compared with the existing biosynthesis method, the recombinant bacteria constructed by using the gene segment of the modified tryptophan enzyme mutant can efficiently produce indole; meanwhile, the indole production method provided by the invention is low in cost, raw materials are easy to obtain, and the production cost is greatly reduced; the production method provided by the invention is short in fermentation period, simple in process, high in efficiency and not limited by regions. Therefore, the tryptophan mutant and the indole production method provided by the invention have good industrial practicability.

The invention relates to the field of microorganisms, and discloses a microbial composite inoculant and a method for degrading lignocellulose. The compound microbial agent comprises phanerochaete chrysosporium, aspergillus niger, trichoderma reesei, neurospora crassa and gloeophyllum trabeum. The phanerochaete chrysosporium is a white-rot fungus with strong performance, and can secrete a plurality of lignocelluloses at the same time. Aspergillus niger, trichoderma reesei, neurospora crassa and gloeophyllum trabeum are mainly used for secreting high-activity cellulase and hemicellulase. Destroying the lignocellulose structure needs the combined action of various lignocellulose enzyme systems, and after the five strains are mixed, the synergistic effect among the enzyme systems in the system can be remarkably enhanced, the lignocellulose structure is efficiently destroyed, and the degradation rate of the wood fiber raw material is increased.

The invention discloses a low-salt rapid bamboo shoot fermentation method, which adopts an inoculated fermentation mode, uses lactobacillus plantarum and lactobacillus brevis as leavening agents, and uses fresh bamboo shoots as raw materials. The preparation method comprises the following steps: selecting fresh bamboo shoots with high tenderness, peeling off bamboo shoot shells, cutting off bases, cleaning, and cutting into bamboo shoot slices with uniform sizes; cooking in boiling water for 5 minutes, fishing out, and airing to remove moisture; preparing water with the salt concentration of 2% and the glucose concentration of 2%, boiling the water, and airing the water for later use to obtain a fermentation feed liquid; adding the air-dried fresh bamboo shoot slices and the prepared material liquid into a bag which is scalded by boiling water and cooled according to a solid-to-liquid ratio of 1: 4, and then adding a lactic acid bacteria leavening agent into the fermentation bag for sealed fermentation; and placing the fermentation bag in an environment with a temperature of 30 DEG C for fermentation for 5 days. The prepared fermented bamboo shoots are short in fermentation period, low in nitrite content, mellow and normal in sourness, rich in flavor and high in sensory quality and food safety, and can better meet industrial and consumer requirements.

According to the livestock and poultry manure stacking fermentation device, a stacking box body is stacked, a heat preservation box body is arranged outside the stacking box body, the material stacking amount is increased, meanwhile, the occupied area is small, stacking of different layers can be conducted according to the types of different fermentation materials, for example, pig manure is low in organic matter content, many in crude fiber, short in fermentation period and small in layer number; the chicken manure is high in organic matter content, long in fermentation period and large in layer number; sludge is complex in organic matter component, high in organic matter content, long in fermentation period and large in layer number. Materials are subjected to multi-layer fermentation stirring in the heat preservation box body, heat preservation treatment is carried out through the heat preservation box body, the fermentation effect is improved, meanwhile, air inlet holes are formed in the lower portion, exhaust holes are formed in the upper portion, and under the influence of heat generated by fermentation, air at the lower portion rises and is exhausted through the exhaust holes, so that external air enters through the air inlet holes at the lower portion; air flow is formed, air is supplemented into the heat preservation box body, and enough oxygen is provided for fermentation.

The invention belongs to the technical field of microorganisms, and particularly relates to leuconostoc mesenteroides, fermented spicy cabbages with the effects of soothing the nerves, helping sleep and relieving anxiety and a preparation method of the fermented spicy cabbages. The leuconostoc mesenteroides NCU018046 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 5, 2024, and the preservation number of the leuconostoc mesenteroides NCU018046 is CGMCC NO.31870. The strain has strong acid and cholate resistance, has strong inhibition capability on common food-borne pathogenic bacteria, and meets safety standards. The complex microbial inoculant provided by the invention is prepared from the strain and plant lactobacillus NCU0011098. The fermented spicy cabbages prepared by fermenting a single bacterial agent of leuconostoc mesenteroides NCU018046 or the complex bacterial agent are good in flavor and low in content of harmful substances, and have various biological activities of soothing the nerves, helping sleep, relieving anxiety, resisting hypertension, resisting oxidation, reducing uric acid, resisting colon cancer and the like.

The present invention is applicable to the field of microbial technology, and provides Klebsiella sp. YCTU3162 and the extracellular polysaccharide Flocan produced by it and its application. Klebsiella sp. YCTU3162 has a deposit number of CCTCC NO: M 20232027 and a deposit date of October 25, 2023. The extracellular polysaccharide Flocan is obtained by the production of Klebsiella sp. YCTU3162. The extracellular polysaccharide Flocan includes glucuronic acid, glucose, mannose and galactose in a molar ratio of 1:2:3:1, and has a pyruvic acid modification group. The production method of the extracellular polysaccharide Flocan in the present invention is simple and easy, with a short fermentation cycle and mild conditions. It has good flocculation activity in synergistic action with metal ions under acidic conditions for simulated kaolin and coal ash wastewater, is biodegradable, has no secondary pollution, and has good economic and social benefits.

The invention discloses a fermented mutton sausage rich in dietary fiber and a preparation method thereof, and the preparation method is characterized in that dietary fiber is added into mutton, so that intestinal flora can be improved, intestinal absorption can be promoted, and the nutritional value of the mutton sausage is improved. The preparation technology and formula of the sausage are optimized through a single factor test, the texture property of the sausage is improved, the fermentation period in the preparation process is short, the cooking loss rate is low when the sausage is processed, and the prepared sausage has good quality, compact taste, elasticity and unique flavor.

This invention discloses a heat-stable bovine alpha-interferon mutant, the amino acid sequence of which is shown in SEQ ID NO.7. The mutant introduces cysteine ​​mutations at amino acid positions 55 and 112, forming an S55C+L112C disulfide bond, compared to wild-type bovine alpha-interferon; the amino acid sequence of wild-type bovine alpha-interferon is shown in SEQ ID NO.1. This invention is the first to propose a disulfide bond modification scheme for bovine alpha-interferon that balances heat stability and antiviral activity, adapted to the Pichia pastoris high-efficiency secretory expression system. The modified mutant exhibits 14.7-fold increased antiviral activity compared to the wild type. At 56°C for 1 hour, the mutant shows significantly improved heat resistance, with an antiviral activity reaching 1.0 × 10⁻⁶. 7.0 IU / ml. The activity is stable after long-term storage at room temperature or 37°C. After storage at room temperature for six months and at 37°C for two months, the antiviral activity still retains 1.0 × 10⁻⁶. 6.0 IU / ml, thus solving the problems of poor stability, easy inactivation under high temperature conditions, and difficulty in transportation at room temperature of wild-type bovine alpha-interferon.

The invention discloses a production process of an apricot kernel fermentation oral liquid. The production process comprises the following steps: step 1, obtaining an intermediate product apricot kernel oil cake prepared from apricot kernel oil; 2, the almond oil cakes are put into a hopper of a coarse crusher, the crusher is started for coarse crushing, and the oil cakes subjected to coarse crushing are required to pass through a screen with the aperture of 3 mm and then are subjected to micro-crushing; carrying out micro-crushing on the roughly crushed oil cake by using a universal crusher, and passing through a 80-mesh sieve; 3, 740 kg of water is added into a multifunctional extraction tank, a stirrer is started, the mixture is heated to 40 DEG C, and 100 kg of the micronized almond oil cakes are put into the multifunctional extraction tank to be dissolved; continuously stirring so that the oil cake and the water are fully mixed and uniformly soaked for 30-40 minutes; the almond fermented oral liquid is prepared from an intermediate product almond oil cake processed by almond oil, and the almond oil cake is obtained by adopting a cold oil pressing technology, so that most of nutrient substances of almonds are reserved, and the residual intermediate product in almond oil processing is fully utilized.

This invention provides a proline-producing strain, its construction method, and its applications. The strain is derived from a starting strain using metabolic engineering techniques. E. coli It was obtained through further modifications based on W3110, specifically: knockout. putA、aceA、poxB、 ldhA、ackA、ptsG Genes, upregulated proB74 , proC、 proA、sthA、glf、glk、gdhA The transcriptional level of the gene was downregulated. SucAB The transcriptional level of genes, affecting phosphorylketonease genes BAD_0687 and proline transporter encoding genes cgl2622 Heterologous expression was performed; the strain used glucose as a carbon source, requiring no substrate addition, resulting in low production costs, high production rate, short fermentation cycle, and high strain stability, thus offering significant economic benefits and laying the foundation for large-scale proline production.

This invention discloses a recombinant *Escherichia coli* strain, its preparation method and application, and a method for synthesizing oryzanol A, relating to the field of bioengineering technology. The recombinant *E. coli* strain uses *E. coli* BL21(DE3) as the chassis cell and undergoes the following modifications (a) and (b): (a) knocking out the pfkA gene; or, knocking out the pfkA gene and mutating the 5′ untranslated region of the gltA gene from the sequence shown in SEQ ID NO: 1 to the sequence shown in SEQ ID NO: 2; (b) inserting exogenous genes agmA, agmB, agmC, agmE, and agmF. This recombinant *E. coli* strain exhibits rapid growth, a short fermentation cycle (within 72 hours), can utilize inexpensive and readily available culture media, and significantly increases the fermentation yield of oryzanol A compared to existing *E. coli* systems.

The invention belongs to the field of gene engineering and enzyme engineering, and particularly relates to an activity-improved UDP glycosyl transferase mutant and application thereof. The glycosyl transferase mutant is a four-combination mutant N2HN1I, and the amino acid sequence of the glycosyl transferase mutant is as shown in SEQ ID NO. 4. The mutant provided by the invention has higher catalytic activity, the conversion rate of a substrate crocetin is high, and the mutant has important industrial application value.

This invention proposes a method for preparing selenomethionine, comprising the following steps: S1. Inoculating selenium-resistant Bacillus subtilis into a culture medium containing sodium selenite for activation, and selecting seed culture in the logarithmic growth phase; S2. Inoculating the seed culture into a fermentation medium, providing carbon and nitrogen sources for cell proliferation in the early stage of fermentation, and adding inorganic selenium source and exogenous methionine precursor in the middle and late stages of fermentation, while adjusting dissolved oxygen and pH to reduce inorganic selenium into selenomethionine within the cells; S3. After fermentation, subjecting the fermentation broth to cell disruption, solid-liquid separation, and drying to obtain a product rich in selenomethionine. This preparation method, through staged fermentation, balances cell growth and selenium conversion efficiency, achieving efficient synthesis of selenomethionine from Bacillus subtilis. The product contains selenium primarily in the form of selenomethionine, with a high proportion of organic selenium. The strains used are highly safe, the process is mature, and the application value is significantly improved, making it suitable for industrial-scale production.

The invention relates to the technical field of wine brewing, and provides an artificial pit mud wet culture method. The method comprises the following steps: S1, collecting a sample from old pit mud, and separating to obtain an anaerobic strain; s2, respectively carrying out activation culture and adaptive pre-culture on the separated strains; s3, preparing a pit mud matrix, and performing nutrition activation treatment; s4, uniformly planting the activated mixed flora on the substrate, and slightly stirring to ensure uniform distribution; s5, dynamically controlling fermentation in stages in the fermentation process until the pit mud reaches a mature state; and S6, performing real-time monitoring and component detection on the fermentation process. Core microorganisms such as anaerobic bacillus, lactic acid bacteria and methane-oxidizing bacteria are separated and activated from the aged pit mud, the technical effect of rapidly establishing a high-activity flora ecosystem is achieved through the technical scheme of activation culture and adaptive pre-culture, the defects that the flora colonization speed is low, and the microbial ecosystem is unstable are overcome, and the method is suitable for large-scale popularization and application. The mature period of the new pit mud is obviously shortened.

This invention discloses a method for fermenting fresh almond flowers with *Lactobacillus plantarum*, the fermentation products, derivatives, and their applications. The method uses fresh almond flowers as raw material. After pretreatment, pulping, substrate preparation, and sterilization, *Lactobacillus plantarum* is inoculated for anaerobic or microaerobic fermentation. After fermentation, enzymes are inactivated and separated to obtain the fermentation expression product. This fermentation expression product is rich in free amino acids, small peptides, aromatic compounds, organic acid derivatives, and polyphenol derivatives, exhibiting a mild and natural aroma and excellent antioxidant and soothing activities. This invention also provides derivatives obtained from further processing of this fermentation expression product, and their applications in cosmetics, skincare products, natural fragrances, and functional foods. This invention features a mild process, is solvent-free, and highly safe, achieving high-value utilization of fresh almond flowers.

The application discloses a sludge anaerobic acid production strengthening method based on synergistic degradation of melanoid-like substances by thermal hydrolysis and potassium permanganate, which comprises the following steps: (1) screening and removing impurities from sludge, and then performing gravity sedimentation and concentration; (2) thermal hydrolysis treatment: high-temperature and high-pressure treatment, pressure relief and cooling; (3) adding potassium permanganate for oxidation, and combining with ultrasonic dispersion; (4) anaerobic fermentation: inoculating active sludge, and controlling fermentation conditions; and (5) separating and extracting short-chain fatty acids. The application realizes the synergistic effect of thermal hydrolysis and potassium permanganate, targets the degradation of melanoid-like substances generated in the thermal hydrolysis process, eliminates the inhibition of the melanoid-like substances on anaerobic microorganisms, and promotes the efficient dissolution of organic matters in sludge. The nano manganese dioxide generated by potassium permanganate oxidation serves as an electron transfer medium, further strengthens the metabolic activity of acid-producing bacteria, and the pickling recovery rate is greater than or equal to 90%. The method makes the yield of short-chain fatty acids increase by 65% (up to 456 mg / g VS), has high efficiency and environmental protection, and provides an innovative solution for sludge resource treatment.

This invention discloses a method for preparing iron-zinc-selenium-enriched yeast, comprising the following steps: (1) adding sterilized inorganic iron solution and inorganic zinc solution to sterilized YPD culture medium, so that the Fe in the culture medium is enriched. 2+ The final concentration is 150-300 mg / L, Zn 2+ The final concentration is 100-300 mg / L, and it is stored at 4℃ for later use; (2) The activated yeast is inoculated into the above culture medium at an inoculation rate of 10%, the initial pH of the culture medium is adjusted to 6-8, the rotation speed is 150-200 r / min, the liquid volume is (50-80) mL / 250 mL, and it is cultured at 28-32℃. When the culture reaches 6 h, sterilized inorganic selenium solution is added to the culture medium to make the Se in the culture medium more concentrated. 2+ The final concentration is 15-30 mg / L, and the culture is continued for (24-48) h. The bacterial cells are collected by centrifugation; (3) The collected bacterial cells are washed and dried to obtain iron, zinc and selenium-enriched yeast. This invention prepares a yeast that can simultaneously obtain iron-enriched yeast, zinc-enriched yeast and selenium-enriched yeast. It has a short fermentation cycle, is easy to cultivate, has a simple process, good stability, high conversion rate of organic iron, zinc and selenium, and is easy to process and store.

This invention relates to a genetically engineered strain for synthesizing retinal, its construction method, and its applications. The construction method involves introducing DNA fragments of the β-carotene 15,15'-oxygenase gene, isopentenyl pyrophosphate isomerase gene, phytoene dehydrogenase gene, geranyl-geranyl pyrophosphate synthase gene, and phytoene synthase gene into *Rhizopus cylindrica* to obtain a genetically engineered strain. This strain enhances the expression of β-carotene 15,15'-oxygenase, isopentenyl pyrophosphate isomerase, phytoene dehydrogenase, geranyl-geranyl pyrophosphate synthase, and phytoene synthase in *Rhizopus cylindrica*. By selecting *Rhizopus cylindrica* as a host, introducing key synthetic genes, and achieving stable genome integration, this method yields an engineered strain capable of efficiently synthesizing retinal using glucose as a carbon source, simplifying the production process, reducing costs, and improving industrial applicability.

The invention relates to the technical field of genetic engineering, in particular to stable recombinant XVII type collagen and application thereof. The stable recombinant XVII type collagen has a stable molecular structure, the molecular weight of the stable recombinant XVII type collagen is smaller than that of a complete sequence, and the stable recombinant XVII type collagen contains functional active sites and can give consideration to transdermal absorption and biological structure stability; the stable recombinant XVII type collagen can realize industrial production, and has good application in the fields of medicines, medical equipment, biological materials, tissue engineering products, cosmetics or health care products and the like.