45 results about "Geranyl pyrophosphate" patented technology

The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and / or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

The invention provides an Escherichia coli expression strain for high yield of geraniol glucoside, and belongs to the technical field of genetic engineering. The Escherichia coli expression strain jointly expresses an acetoacetyl-CoA thiolase gene, a methyl glutaryl coenzyme A synthetase gene, a HMG-CoA reductase gene, a mevalonate kinase gene, phosphomevalonate kinase gene, a pyrophosphate mevalonate decarboxylase gene, an isopentenyl diphosphate isomerase gene, a geranyl pyrophosphate GPP synthetase ispA*, a geraniol synthetase gene, a geranyl pyrophosphate GPP synthetase ERG20* and a glycosyltransferase gene. The yield of geraniol glucoside produced from the Escherichia coli expression strain is high, reliable in mass and good in application prospect.

This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyioin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds. These condensation reactions serve as a platform for the synthesis of isoprenoid precursors when utilized in combination with a variety of metabolic pathways and enzymes for carbon rearrangement and the addition / removal of functional groups. Isoprenoid alcohols are key intermediary products for the production of isoprenoid precursors in these novel synthetic metabolic pathways.

The invention provides a geranyl pyrophosphate synthase gene from anoectochilus roxburghii and an application of the geranyl pyrophosphate synthase gene from anoectochilus roxburghii, and relates to the technical field of genetic engineering. The geranyl pyrophosphate synthase gene has a nucleotide sequence as shown in SEQID NO.1, and an amino acid sequence as shown in SEQID NO.2. An important basis is provided for variety breeding of the anoectochilus roxburghii, and a base is provided for researching terpenoid enriched transgenic plant strains.

The present invention provides a method for producing stevioside compounds by microorganism, comprising carrying out heterologous biosynthesis of stevioside compounds from geranyl geranyl pyrophosphate synthase (GGPPS), Copalyl pyrophosphate synthase (CDPS), Kaurene synthase (KS), dual-function kaurene synthase (CPS / KS), kaurene oxidase (KO), a cytochrome P450 redox protein (CPR), kaurenoic acid-13[alpha]-hydroxylase, UGT85C2 glycosyltransferase and UGTB1 / IBGT glycosyltransferase (optionally comprising UGT74G1 glycosyltransferase and / or UGT76G1 glycosyltransferase).

The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.

The present invention relates to a process of producing a monoterpene and / or derivatives thereof. The process comprises the steps of: a) providing a host microorganism genetically engineered to express a bacterial monoterpene synthase (mTS); and b) contacting geranyl pyrophosphate (GPP) with said bacterial mTS to produce said monoterpene and / or derivatives thereof. The present invention also relates to a microorganism for use in producing a monoterpene and / or derivatives thereof and a recombinant microor-ganism adapted to conduct the step of converting geranyl pyrophosphate (GPP) into a monoterpene and / or derivatives thereof by ex-pression of a bacterial mTS. It was shown to produce 1,8 cineole using 1,8 cineole synthase and to produce linalool using linalool synthase, both from Streptomyces clavuligerus.

According to the isopentenyl transferase mutant and the method for producing cannabinoid phenol, existing isopentenyl transferase is subjected to site-directed mutagenesis, so that olive alcohol and geranyl pyrophosphate can be helped to catalyze to generate cannabinoid phenol with relatively high catalytic activity and purity, and the isopentenyl transferase mutant has relatively high production and application prospects.

The invention discloses an engineering bacterium for co-producing geraniol and nerol, a construction method and application thereof, and belongs to the technical field of biological engineering. The construction method of the engineering bacteria comprises the following steps: introducing tobacco geraniol and nerol synthase genes into a recipient strain capable of producing geranyl pyrophosphate to obtain recombinant bacteria; obtaining the recombinant bacteria from the recombinant bacteria Engineered bacteria for the production of geraniol and nerol. The present invention successfully utilizes the bioengineering technology to realize the heterologous reconstruction of the biosynthetic pathway of geraniol and nerol and the co-production of the products, and the yields of geraniol and nerol by the shake flask fermentation method reach 137.3 mg / L and 62.8 mg / L respectively. ; At the same time and through the high-density fermentation technology of recombinant strains, the yields of geraniol and nerol were increased by about 10 times.

The invention provides a method for preparing an intermediate of acetonyl geranyl pyrophosphate. Farnesol is used as a raw material, hydroxyl group is protected, and then an epoxy intermediate is prepared, which is cut by epoxy to form an aldehyde, and then mixed with methyl Grignard reagent. Reaction, re-oxidation of hydroxyl group, deprotection to obtain (5E, 9E)-11-hydroxyl-5,9-dimethylundec-5,9-dien-2-ketone 7, then hydroxy halogenation, then with pyrophosphoric acid A salt reaction is performed to finally obtain acetonyl geranyl pyrophosphate. The method is cheap to synthesize, has mild reaction conditions, and has less isomer impurities, and is suitable for scale-up production.

The invention discloses a oleifera sesquiterpene synthase SgSTPS2 and its coding gene and application. The present invention provides a new sesquiterpene synthase, SgSTPS2 and its coding gene, the protein can generate active sesquiterpene synthase after prokaryotic expression, and catalyze farnesyl pyrophosphate (FPP) or geranyl pyrophosphate (GPP) to generate a variety of sesquiterpenoids or monoterpenoids, which can be used for their mass production.

The invention discloses a method for detecting the content of geranyl pyrophosphate. The method comprises the steps of: firstly, purifying and enriching a to-be-detected sample, designing and synthesizing two fluorescent peptides that generate a prenylation reaction with geranyl pyrophosphate according to the principle of the prenylation reaction, respectively adding the two peptides into a to-be-detected sample solution and a solution containing a fixed amount of geranyl pyrophosphate pure product to generate the prenylation reaction to respectively obtain a target compound and an internal standard substance for indirectly detecting the geranyl pyrophosphate, detecting the target compound and the internal standard substance by using a high performance liquid chromatograph-fluorescence detector to obtain an initial detection value of the geranyl pyrophosphate, designing a recovery experiment to calculate the mass of the geranyl pyrophosphate that is lost when the to-be-detected sampleis purified, and establishing a recovery calibration curve to correct an initial measured value to finally obtain the content of the geranyl pyrophosphate. The method provided by the invention is stable and reliable, and has high reproducibility, and the applicable scope covers the detection of geranyl pyrophosphate in different organisms.

The invention provides a geranyl pyrophosphate synthase gene derived from Fujian Aureus aureus and its application, and relates to the technical field of genetic engineering. The geranyl pyrophosphate synthase gene has the nucleotide sequence shown in SEQ ID NO.1 and the amino acid sequence shown in SEQ ID NO.2. The invention provides an important basis for the selection and breeding of clematis varieties, and also provides a basis for the research on transgenic plant strains enriched in terpenoids.

To provide a series of techniques for obtaining β-phellandrene with high purity and in a large quantity.Provided is a recombinant cell capable of producing β-phellandrene, prepared by introducing at least one nucleic acid selected from the group consisting of a nucleic acid encoding geranyl pyrophosphate (GPP) synthase and a nucleic acid encoding neryl pyrophosphate (NPP) synthase, and a nucleic acid encoding β-phellandrene synthase into a host cell in such a manner that these nucleic acids are expressed in the host cell. Also provided is a method for producing β-phellandrene by culturing the recombinant cell to produce β-phellandrene in the recombinant cell.