1205 results about "Pyrophosphoric acid" patented technology

Convenient techniques for discriminating the base type in a base sequence of a nucleic acid are provided. The technique includes the step (a) of preparing a sample solution containing a nucleic acid, a primer having a base sequence that includes a complementary binding region which complementarily binds to the nucleic acid, and a nucleotide; the step (b) of allowing the sample solution to stand under a condition to cause an extension reaction of the primer, and producing pyrophosphate when the extension reaction is caused; the step (c) of bringing the sample solution into contact with the front face of a H+ hardly permeable membrane having H+-pyrophosphatase, which penetrates from front to back of the membrane, of which active site that hydrolyzes pyrophosphate being exposed to the front face; the step (d) of measuring the H+ concentration of at least either one of the solution at the front face side of the H+ hardly permeable membrane or the solution at the back face side of the H+ hardly permeable membrane, in a state where the H+-pyrophosphatase is immersed in the solution; the step (e) of detecting the extension reaction on the basis of the result of measurement in the step (d) ; and the step (f) of discriminating the base type in the base sequence of the nucleic acid on the basis of the result of detection in the step (e).

Processes are disclosed using the depolymerization of a nucleic acid hybrid and incorporation of a suitable nucleotide to qualitatively and quantitatively analyze for the presence of predetermined nucleic acid target sequences. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, genotyping, medical marker diagnostics, mirosequencing, and.

The invention relates to an improver for saline-alkali soil. The improver comprises a powder agent and a water agent, wherein the powder agent comprises the following components: organic matters, plant mycelium protein powder, active calcium, calcium superphosphate, aluminum sulfate, fatty alcohol polyoxyethylene ether ammonium sulfate, amino acid powder, bacillus subtilis and photosynthetic bacteria, and the water agent comprises the following components: citric acid, fulvic acid, humic acid, malic acid, maleic acid, fumaric acid, L-tartaric acid, pyrophosphoric acid, humic acid, lactic acid, oxalic acid, salicylic acid, sodium ion adsorbent, Tween-80 and water. The improver disclosed by the invention can improve the soil from multiple angles, namely soil structure, microorganism species, a chemical method and the like, obviously promote the release of iron and phosphorus in the soil, improve the soil structure, effectively reduce the content of sodium ions in the soil, reduce the pH value of the soil and restore land to a normal planting state after being used continuously for two years.

The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and / or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.

Methods for synthesizing amorpha-4,11-diene synthase from isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. Amorpha-4,11-diene synthase is then produced using an optimized amorpha-4,11-diene synthase gene. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

The present invention provides a pyrophosphoric acid bath for use in Cu-Sn alloy plating without containing a cyanic ion comprising a reaction product (A) of an amine derivative and an epihalohydrin in a 1:1 mole ratio and a cationic surfactant (B) and, when necessary, further comprising a surface tension adjusting agent (C), a bath stabilizer (D) and an N-benzylpyridinium derivative (E) as additives. According to the bath of the present invention, by changing a bath composition of copper and tin or by employing a characteristic additive, a stable film with a silver-white, gold, copper or light black color can be obtained. By increasing tin contents in the bath, the bath can be used for lead-free solder plating. The bath is safe in handling and hygienic and, moreover, has no sewage process and environmental problems since it contains neither cyanic compound nor formaldehyde derivative.

The invention discloses a method for producing a flame-retardant veneer plywood, which comprises the following steps: placing wood veneers into a flame retardant aqueous solution to perform dipping treatment, applying the mixed adhesive to the upper surfaces and the lower surfaces of the veneers after the veneers are dried, assembling the veneers into a plate blank according to the principle that odd layers are crossed and symmetrical with even layers, and then performing the processes of cold pressing, hot pressing, cutting edge, sanding, surface treating and the like to obtain the flame-retardant veneer plywood product the thickness of which is between 2.7 and 20 millimeters. The flame retardant in the method is a mixture of low ammonium polyphosphate, ammonium phosphate, ammonium pyrophosphate, urea phosphate, boric acid and pentaerythritol, and the mixed adhesive is a uniform mixture of wood adhesive, filler, and a curing agent. The flame-retardant decorative veneer plywood prepared by the method has the advantages of excellent flame-retardant and fireproof performance, environmental prevention performance, and good mechanical properties.

Oral care compositions containing a stannous salt and sodium tripolyphosphate are provided where stannous tripolyphosphate ionic intermediates comprise less than about 1% of the compositions Tetrasodium pyrophosphate, a methylvinyl ether-maleic anhydride copolymer and / or a silica oral polishing agent may be added for further efficacy. In a single phase blend, the oral care composition may be effectively limited to comprise less than about 10% water. In a dual-phase blend a mixture having the stannous salt in a first phase and the sodium tripolyphosphate in a second phase may be generated from a dual chamber storage tube (with each phase provided from a separate chamber) immediately prior to dental care usage.

A highly water soluble ferric pyrophosphate citrate chelate useful for treating iron deficiency contains 2% or less phosphate by weight. These chelate compositions are easily milled and / or processed into dosage forms using conventional techniques, and are expected to exhibit advantageous biocompatibility as compared to conventional soluble ferric pyrophosphates, ferric salts, ferric polysaccharide complexes and ferrous salts.

The invention provides a flame retardant composition comprising 1-99 weight parts of a salt of piperazine and an inorganic compound selected from among piperazine phosphate, piperazine pyrophosphate and piperazine polyphosphate, or a mixture of two or more of these piperazine salts (ingredient (A)), 99-1 weight parts of a salt of melamine and an inorganic compound selected from among melamine phosphate, melamine pyrophosphate and melamine polyphosphate, or a mixture of two or more of these melamine salts (ingredient (B) ) (wherein, the sum of ingredient (A) and ingredient (B) is 100 weight parts), 0-50 weight parts of an arbitrary ingredient (ingredient (C)), and 0.01-20 weight parts of a silicone oil having a viscosity at 25° C. of 5000 mm2 / s (ingredient (D)) which is added thereto. This flame retardant not only has superior flame retarding properties, but also has enhanced powder properties and anti-hygroscopic properties, and when it is added to a resin, there is little change of electrical resistance.

A stable and efficacious antiplaque, anticalculus, and antigingivitis oral composition is provided having an antibacterial ingredient comprising one or more active compounds found in an extract of magnolia combined with an anticalculus system comprising tetrasodium pyrophosphate (TSPP) about 1 to about 2.5% and sodium tripolyphosphate (STPP) about 1 to about 10%. The oral composition can be in the form of a mouth rinse or dentifrice, including toothpaste, gels, powders, as well as confectionaries, lozenges, and the like. Methods of making and using the oral composition are also provided.

A palatability enhancing composition for extruded pet food containing at least one potassium pyrophosphate salt in an amount effective to enhance palatability and at least one ingredient selected from animal products, animal by-products, fish products, fish by-products, dairy products, dairy by-products, sources of microbial proteins, vegetable proteins, carbohydrates and amino acids. Methods for enhancing pet food palatability with the disclosed palatability enhancing compositions and extruded pet food compositions coated therewith are also disclosed.

Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

This invention provides methods for pyrosequencing and compositions comprising 3′-O— modified deoxynucleoside triphosphates.

Disclosed is a DNA analysis method and a DNA analyzer whose signal intensity is not lowered even when a material at a higher density is measured. There is supplied dATP, dTTP, dGTP, or dCTP from a dATP solution vessel, a dTTP solution vessel, a dGTP solution vessel, or a dCTP solution vessel, and this causes an extension reaction of a double-stranded DNA immobilized to a bead, to yield pyrophosphoric acid. The pyrophosphoric acid is converted into a redox compound by the actions of a reagent and an enzyme contained in a reaction buffer in a reaction buffer vessel. The redox compound causes a variation in surface potential of a measuring electrode bearing an electrochemically active material immobilized thereto through an insulating molecule, and this variation causes a variation in drain current of a field-effect transistor electrically connected to the measuring electrode. Thus, the extension reaction is detected.

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

The invention relates to a pyrophosphoric acid bath for use in cyanogen-free copper-tin alloy plating that contains an additive (A) composed an amine derivative, an epihalohydrin and a glycidyl ether compound with ratios of epihalohydrin to glycidyl ether compound being 0.5-2 to 0.1-5 on mol basis, per 1 mol of the amine derivative, has a pH of 3 to 9, and optionally contains an additive (B) composed of an organic sulfonic acid and / or an organic sulfonic acid salt, and to a copper-tin alloy coating obtainable by using the bath. The invention provides a pyrophosphoric acid bath for use in copper-tin alloy plating of the cyanogen-free type utilizable on an industrial scale, particularly, capable of performing uniform treatment to exhibit low defective product generation rates even with the current density being incessantly changing between a high state and a low state, as a barrel plating method, and a copper-tin alloy coating obtainable by using the bath.

The present invention provides a composite flame retardant which can be produced without using any toxic metal such as antimony trioxide or any halogen such as bromine and chlorine and can exhibit excellent flame retardancy compared with magnesium hydroxide when added in a smaller amount; and a flame retardant resin composition having excellent flame retardancy and excellent physical properties. The present invention relates to a flame retardant including: (A) 100 parts by mass of a reaction product of piperazine with one phosphorus compound selected from phosphoric acid, pyrophosphoric acid, and polyphosphoric acid; (B) 10 to 1000 parts by mass of a reaction product of melamine with a polyacid selected from cyanuric acid, phosphoric acid, pyrophosphoric acid, and polyphosphoric acid; and (C) 0.1 to 100 parts by mass of a reaction product of calcium or magnesium with silicic acid.

The present invention relates to flame resistant polyester resin compositions comprising 30 to 90 weight percent thermoplastic polyester; 1 to 30 weight percent oligomeric aromatic phosphate ester; 1 to 25 weight percent phenolic polymer; 1 to 35 weight percent of at least one melamine flame retardant selected from melamine pyrophosphate, melamine phosphate, melamine polyphosphate, melamine cyanurate, and mixtures thereof; and optionally inorganic reinforcing agents. The present invention further relates to molded articles or parts formed from resins comprising such flame resistant polyester resin compositions, and the laser welded articles further produced therefrom.

The invention relates to a polytetrafluoroethylene (PTFE) composite material sealing ring with high temperature resistance and low creep, and a preparation method thereof. The PTFE and carbon fiber are subjected to pressure forming, high temperature sintering and heat treatment to prepare the composite material sealing ring. The carbon fiber is prepared through the following steps that: a pyrophosphoric acid-type titanic acid ester coupling agent is adopted for carrying out a surface coating treatment for the carbon fiber; then the treated carbon fiber is subjected to an oxidation treatment for 5-20 minutes at a temperature of 350-450 DEG C. According to the present invention, the interfacial combining capacity between the inorganic fiber and the PTFE resin is substantially improved, the mechanical property and the creep resistance of the modified sealing material are increased.

The present invention relates to a catalyst made up by using zirconium base carrier material or zirconium base carrier material modified by phosphoric acid and vanadium phosphorus oxide loaded on said carrier material. The described zirconium base carrier material is zirconium oxide, the loaded quantity of vanadium phosphorus oxide is 25-47 wt% of total mass of said catalyst, in which the atomic ratio of vanadium and phosphorus is 1.2, its specific surface area is about 21-29 sq.m / g. When it is used as catalyst for normal-butane air oxidation reaction to prepare cis-butenedioic anhydride, in the typical reaction temperature zone of 380-420deg.C its per pass conversion rate is 38-89%, the cis-butenedioic anhydride selectivity is 29-69% and maximum cis-butenedioic anhydride yield is 61.2%. Said invention also discloses the preparation method of said catalyst.

The catalyst consists of loaded vanadium and phosphor oxide and SiO2 carrier formed via pyrogenic decomposition with the loaded amount being 15-58 wt%. It has a V / P atom ratio of 1.2, specific area 117-210 sq m / g, and the main phase being vanadyl pyrophosphate phase. It is used as the catalyst for air oxidizing n-butane to prepare cis-butenedioic anhydride and has one single-path conversion rate of 33-51 % and cis-butenedioic anhydride selectivity of 61-87 % in the typical temperature range of 380-400 deg.c.

Corrosion-inhibited fire retardant compositions and methods of making and using the same are provided. The corrosion-inhibited fire retardant compositions are comprised of at least one fire retardant component, at least one biopolymer having a particle size diameter of less than about 100 microns, and a corrosion inhibiting system. The corrosion inhibiting system is comprised of at least one corrosion inhibiting compound selected from a group of compounds including azoles, insoluble ferric pyrophosphate, soluble ferric pyrophosphate, ferrous oxalate, ferric citrate, ferrous sulfate, ferric ammonium citrate, soluble ferric orthophosphate, insoluble ferric orthophosphate, ferric ammonium oxalate, ferric ammonium sulfate, ferric bromide, ferric sodium oxalate, ferric stearate, ferric sulfate, ferrous acetate, ferrous ammonium sulfate, ferrous bromide, ferrous gluconate, ferrous iodide, ferric acetate, ferric fluoroborate, ferric hydroxide, ferric oleate, ferrous fumarate, ferrous oxide, ferric lactate, ferric resinate and any combination thereof. In a specific embodiment, the corrosion-inhibited fire retardant composition includes a xanthan biopolymer.

The invention relates to a fluorescent DNA-silver nanocluster, and a preparation method and application thereof. The particle size of the fluorescent DNA-silver nanocluster is 2-10nm. A water solution of the fluorescent DNA-silver nanocluster looks light pink by visual inspection, and looks shiny red under irradiation of an ultraviolet lamp with the wavelength of 360nm. The water solution is excited by light with the excitation wavelength of 530nm, the emission wavelength is 570-800nm, and high fluorescence emission strength is acquired at the wavelength of 618nm; rhodamine B is taken as a reference substance, and fluorescence quantum yield is 35.3%. A method for detecting cupric ions and pyrophosphate ions by the fluorescent DNA-silver nanocluster includes (1), drawing a response standard curve of the corresponding ions to acquire a linear regression equation; (2), substituting the detected fluorescence strength into the linear regression equation to obtain the concentration of the cupric ions or the pyrophosphate ions. The fluorescent DNA-silver nanocluster has the advantages of stable performance and environment friendliness, the detection method is rapid and simple, and the detection sensitivity is high.

Belonging to the field of flame retardant preparation, the invention discloses a preparation method for a halogen-free flame retardant and a flame retardant polyolefin composition thereof. Specifically, piperazine diphosphate is prepared by reacting phosphoric acid with piperazine in proportion, and then performing spray drying treatment; and piperazine pyrophosphate is prepared by combining the preparation method of the piperazine diphosphate, and subjecting the obtained piperazine diphosphate to dehydration condensation reaction under an inert atmosphere or vacuum condition. The invention also provides the flame-retardant polyolefin composition, which comprises 100 parts by mass of polyolefin resin, 10-100 parts by mass of the piperazine pyrophosphate prepared by the method and the like. The yield of the piperazine diphosphate prepared by the method is over 95%, and the piperazine pyrophosphate has a W value represented whiteness of over 90. The flame retardant polyolefin composition has the advantages of excellent flame retardant properties, small adding amount of piperazine pyrophosphate, and excellent coloring properties, thus having terrific industrial application prospects.

Bioavailable iron-containing parenteral nutrition compositions are provided that are physico-chemically stable. Iron is present in the form of soluble ferric pyrophosphate. Methods of preparation and use of the compositions are provide, as well as kits.

The chemical plating process for magnesium-base material includes the following steps: 1. alkaline dewaxing the magnesium-base material; 2. activating with acid solution containing fluoric salt and no chlorine ion and in pH 2.0-5.0, and treating with alkaline neutralizing solution containing alkali metal salt of pyrophosphoric acid, fluoric salt and hypophosphite in pH 8.0-12.0; 3. chemical nickel pre-plating with the plating solution containing nickel salt in 10-80 g / L concentration, fluoric salt in 5-50 g / L concentration, pyrophosphite in 20-100 g / L concentration and no chlorine ion and in pH 5.0-9.0; 4. chemical nickel plating with the plating solution containing nickel salt in 10-100 g / L concentration, pyrophosphite in 10-80 g / L concentration and no chlorine ion and in pH 3-7; and 5. optional heat treatment. The said process can form homogeneous compact nickel layer with sufficient binding force on magnesium-base material.

Flame retardant compositions, and compositions that comprise an organic polymer and the flame retardant composition are disclosed. The flame retardant compositions are prepared by reacting ethylene diamine with polyphosphoric acid; or reacting an ethyleneamnine or a mixture of ethyleneamines with phosphoric acid, polyphosphoric acid, pyrophosphoric acid, or mixtures thereof. A 10% by weight solution of the product in water has a pH between about 2.5 to 6.0. The flame retardant compositions are non-halogen containing flame retardant compositions and do not gas undesirably during processing at temperatures of 235° C. or even higher.

A stable packaged bicarbonate solution for use in dialysis treatment and for treating anemia, iron deficiency, or reducing the required dose of recombinant erythropoietin to treat anemia includes a bicarbonate solution into which ferric pyrophosphate is dissolved. The amount of ferric pyrophosphate dissolved in the bicarbonate solution is sufficient to provide a therapeutic effect for the treatment of anemia, iron deficiency or to reduce dose of recombinant erythropoietin needed to treat anemia when the bicarbonate solution is combined with other dialysis components to form a dialysate used for dialyzing a patient. The ferric pyrophosphate is stabilized in the bicarbonate solution by holding the solution in a polyolefin container or container liner, such as a high density polyethylene container or liner.