1164 results about "Pyrophosphoric acid" patented technology

Convenient techniques for discriminating the base type in a base sequence of a nucleic acid are provided. The technique includes the step (a) of preparing a sample solution containing a nucleic acid, a primer having a base sequence that includes a complementary binding region which complementarily binds to the nucleic acid, and a nucleotide; the step (b) of allowing the sample solution to stand under a condition to cause an extension reaction of the primer, and producing pyrophosphate when the extension reaction is caused; the step (c) of bringing the sample solution into contact with the front face of a H⁺ hardly permeable membrane having H⁺-pyrophosphatase, which penetrates from front to back of the membrane, of which active site that hydrolyzes pyrophosphate being exposed to the front face; the step (d) of measuring the H⁺ concentration of at least either one of the solution at the front face side of the H⁺ hardly permeable membrane or the solution at the back face side of the H⁺ hardly permeable membrane, in a state where the H⁺-pyrophosphatase is immersed in the solution; the step (e) of detecting the extension reaction on the basis of the result of measurement in the step (d) ; and the step (f) of discriminating the base type in the base sequence of the nucleic acid on the basis of the result of detection in the step (e).

The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.

The present invention provides a pyrophosphoric acid bath for use in Cu-Sn alloy plating without containing a cyanic ion comprising a reaction product (A) of an amine derivative and an epihalohydrin in a 1:1 mole ratio and a cationic surfactant (B) and, when necessary, further comprising a surface tension adjusting agent (C), a bath stabilizer (D) and an N-benzylpyridinium derivative (E) as additives. According to the bath of the present invention, by changing a bath composition of copper and tin or by employing a characteristic additive, a stable film with a silver-white, gold, copper or light black color can be obtained. By increasing tin contents in the bath, the bath can be used for lead-free solder plating. The bath is safe in handling and hygienic and, moreover, has no sewage process and environmental problems since it contains neither cyanic compound nor formaldehyde derivative.

The invention discloses a method for producing a flame-retardant veneer plywood, which comprises the following steps: placing wood veneers into a flame retardant aqueous solution to perform dipping treatment, applying the mixed adhesive to the upper surfaces and the lower surfaces of the veneers after the veneers are dried, assembling the veneers into a plate blank according to the principle that odd layers are crossed and symmetrical with even layers, and then performing the processes of cold pressing, hot pressing, cutting edge, sanding, surface treating and the like to obtain the flame-retardant veneer plywood product the thickness of which is between 2.7 and 20 millimeters. The flame retardant in the method is a mixture of low ammonium polyphosphate, ammonium phosphate, ammonium pyrophosphate, urea phosphate, boric acid and pentaerythritol, and the mixed adhesive is a uniform mixture of wood adhesive, filler, and a curing agent. The flame-retardant decorative veneer plywood prepared by the method has the advantages of excellent flame-retardant and fireproof performance, environmental prevention performance, and good mechanical properties.

Oral care compositions containing a stannous salt and sodium tripolyphosphate are provided where stannous tripolyphosphate ionic intermediates comprise less than about 1% of the compositions Tetrasodium pyrophosphate, a methylvinyl ether-maleic anhydride copolymer and/or a silica oral polishing agent may be added for further efficacy. In a single phase blend, the oral care composition may be effectively limited to comprise less than about 10% water. In a dual-phase blend a mixture having the stannous salt in a first phase and the sodium tripolyphosphate in a second phase may be generated from a dual chamber storage tube (with each phase provided from a separate chamber) immediately prior to dental care usage.

The invention provides a flame retardant composition comprising 1-99 weight parts of a salt of piperazine and an inorganic compound selected from among piperazine phosphate, piperazine pyrophosphate and piperazine polyphosphate, or a mixture of two or more of these piperazine salts (ingredient (A)), 99-1 weight parts of a salt of melamine and an inorganic compound selected from among melamine phosphate, melamine pyrophosphate and melamine polyphosphate, or a mixture of two or more of these melamine salts (ingredient (B) ) (wherein, the sum of ingredient (A) and ingredient (B) is 100 weight parts), 0-50 weight parts of an arbitrary ingredient (ingredient (C)), and 0.01-20 weight parts of a silicone oil having a viscosity at 25° C. of 5000 mm²/s (ingredient (D)) which is added thereto. This flame retardant not only has superior flame retarding properties, but also has enhanced powder properties and anti-hygroscopic properties, and when it is added to a resin, there is little change of electrical resistance.

This invention provides methods for pyrosequencing and compositions comprising 3′-O— modified deoxynucleoside triphosphates.

Disclosed is a DNA analysis method and a DNA analyzer whose signal intensity is not lowered even when a material at a higher density is measured. There is supplied dATP, dTTP, dGTP, or dCTP from a dATP solution vessel, a dTTP solution vessel, a dGTP solution vessel, or a dCTP solution vessel, and this causes an extension reaction of a double-stranded DNA immobilized to a bead, to yield pyrophosphoric acid. The pyrophosphoric acid is converted into a redox compound by the actions of a reagent and an enzyme contained in a reaction buffer in a reaction buffer vessel. The redox compound causes a variation in surface potential of a measuring electrode bearing an electrochemically active material immobilized thereto through an insulating molecule, and this variation causes a variation in drain current of a field-effect transistor electrically connected to the measuring electrode. Thus, the extension reaction is detected.

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

The present invention provides a composite flame retardant which can be produced without using any toxic metal such as antimony trioxide or any halogen such as bromine and chlorine and can exhibit excellent flame retardancy compared with magnesium hydroxide when added in a smaller amount; and a flame retardant resin composition having excellent flame retardancy and excellent physical properties. The present invention relates to a flame retardant including: (A) 100 parts by mass of a reaction product of piperazine with one phosphorus compound selected from phosphoric acid, pyrophosphoric acid, and polyphosphoric acid; (B) 10 to 1000 parts by mass of a reaction product of melamine with a polyacid selected from cyanuric acid, phosphoric acid, pyrophosphoric acid, and polyphosphoric acid; and (C) 0.1 to 100 parts by mass of a reaction product of calcium or magnesium with silicic acid.

The present invention relates to flame resistant polyester resin compositions comprising 30 to 90 weight percent thermoplastic polyester; 1 to 30 weight percent oligomeric aromatic phosphate ester; 1 to 25 weight percent phenolic polymer; 1 to 35 weight percent of at least one melamine flame retardant selected from melamine pyrophosphate, melamine phosphate, melamine polyphosphate, melamine cyanurate, and mixtures thereof; and optionally inorganic reinforcing agents. The present invention further relates to molded articles or parts formed from resins comprising such flame resistant polyester resin compositions, and the laser welded articles further produced therefrom.

The present invention relates to a catalyst made up by using zirconium base carrier material or zirconium base carrier material modified by phosphoric acid and vanadium phosphorus oxide loaded on said carrier material. The described zirconium base carrier material is zirconium oxide, the loaded quantity of vanadium phosphorus oxide is 25-47 wt% of total mass of said catalyst, in which the atomic ratio of vanadium and phosphorus is 1.2, its specific surface area is about 21-29 sq.m/g. When it is used as catalyst for normal-butane air oxidation reaction to prepare cis-butenedioic anhydride, in the typical reaction temperature zone of 380-420deg.C its per pass conversion rate is 38-89%, the cis-butenedioic anhydride selectivity is 29-69% and maximum cis-butenedioic anhydride yield is 61.2%. Said invention also discloses the preparation method of said catalyst.

The invention relates to a fluorescent DNA-silver nanocluster, and a preparation method and application thereof. The particle size of the fluorescent DNA-silver nanocluster is 2-10nm. A water solution of the fluorescent DNA-silver nanocluster looks light pink by visual inspection, and looks shiny red under irradiation of an ultraviolet lamp with the wavelength of 360nm. The water solution is excited by light with the excitation wavelength of 530nm, the emission wavelength is 570-800nm, and high fluorescence emission strength is acquired at the wavelength of 618nm; rhodamine B is taken as a reference substance, and fluorescence quantum yield is 35.3%. A method for detecting cupric ions and pyrophosphate ions by the fluorescent DNA-silver nanocluster includes (1), drawing a response standard curve of the corresponding ions to acquire a linear regression equation; (2), substituting the detected fluorescence strength into the linear regression equation to obtain the concentration of the cupric ions or the pyrophosphate ions. The fluorescent DNA-silver nanocluster has the advantages of stable performance and environment friendliness, the detection method is rapid and simple, and the detection sensitivity is high.

Belonging to the field of flame retardant preparation, the invention discloses a preparation method for a halogen-free flame retardant and a flame retardant polyolefin composition thereof. Specifically, piperazine diphosphate is prepared by reacting phosphoric acid with piperazine in proportion, and then performing spray drying treatment; and piperazine pyrophosphate is prepared by combining the preparation method of the piperazine diphosphate, and subjecting the obtained piperazine diphosphate to dehydration condensation reaction under an inert atmosphere or vacuum condition. The invention also provides the flame-retardant polyolefin composition, which comprises 100 parts by mass of polyolefin resin, 10-100 parts by mass of the piperazine pyrophosphate prepared by the method and the like. The yield of the piperazine diphosphate prepared by the method is over 95%, and the piperazine pyrophosphate has a W value represented whiteness of over 90. The flame retardant polyolefin composition has the advantages of excellent flame retardant properties, small adding amount of piperazine pyrophosphate, and excellent coloring properties, thus having terrific industrial application prospects.

The chemical plating process for magnesium-base material includes the following steps: 1. alkaline dewaxing the magnesium-base material; 2. activating with acid solution containing fluoric salt and no chlorine ion and in pH 2.0-5.0, and treating with alkaline neutralizing solution containing alkali metal salt of pyrophosphoric acid, fluoric salt and hypophosphite in pH 8.0-12.0; 3. chemical nickel pre-plating with the plating solution containing nickel salt in 10-80 g/L concentration, fluoric salt in 5-50 g/L concentration, pyrophosphite in 20-100 g/L concentration and no chlorine ion and in pH 5.0-9.0; 4. chemical nickel plating with the plating solution containing nickel salt in 10-100 g/L concentration, pyrophosphite in 10-80 g/L concentration and no chlorine ion and in pH 3-7; and 5. optional heat treatment. The said process can form homogeneous compact nickel layer with sufficient binding force on magnesium-base material.

Flame retardant compositions, and compositions that comprise an organic polymer and the flame retardant composition are disclosed. The flame retardant compositions are prepared by reacting ethylene diamine with polyphosphoric acid; or reacting an ethyleneamnine or a mixture of ethyleneamines with phosphoric acid, polyphosphoric acid, pyrophosphoric acid, or mixtures thereof. A 10% by weight solution of the product in water has a pH between about 2.5 to 6.0. The flame retardant compositions are non-halogen containing flame retardant compositions and do not gas undesirably during processing at temperatures of 235° C. or even higher.

A stable packaged bicarbonate solution for use in dialysis treatment and for treating anemia, iron deficiency, or reducing the required dose of recombinant erythropoietin to treat anemia includes a bicarbonate solution into which ferric pyrophosphate is dissolved. The amount of ferric pyrophosphate dissolved in the bicarbonate solution is sufficient to provide a therapeutic effect for the treatment of anemia, iron deficiency or to reduce dose of recombinant erythropoietin needed to treat anemia when the bicarbonate solution is combined with other dialysis components to form a dialysate used for dialyzing a patient. The ferric pyrophosphate is stabilized in the bicarbonate solution by holding the solution in a polyolefin container or container liner, such as a high density polyethylene container or liner.

The invention provides the construction for a farnesyl pyrophosphoric acid synthetase RNA (Ribonucleic Acid) interference recombinant lentivirus vector, which comprises the following steps of: sieving the most effective target sequence of an FDS (farnesyl diphosphate synthase) gene RNAi (RNA interference) in a tool cell 293T cell, synthesizing the double-stranded DNA of the most effective target sequence, connecting to a pGCSIL-GFP vector and successfully constructing the recombinant vector through enzyme cutting, sequencing and identification. Researches indicate that the constructed RNA interference vector LV-sh-FDS can downwards modulate the expression of an FDS mRNA (Messenger RNA) level in a neonatal rat cardiac myocyte, simultaneously can downwards modulate the expression of myocardial hypertrophy markers such as cell areas and marker genes beta-MHC (Myosin Heavy Chain) and BNP (Brain Natriuretic Peptide), additionally can effectively inhabit the activity of RhoA while downwards modulating the FDS, can be applied in preparing medicaments for treating myocardial hypertrophy diseases and also can be applied in preparing medicaments for cholesterol metabolic control.

A highly water soluble ferric pyrophosphate citrate chelate useful for treating iron deficiency contains 2% or less phosphate by weight. These chelate compositions are easily milled and/or processed into dosage forms using conventional techniques, and are expected to exhibit advantageous biocompatibility as compared to conventional soluble ferric pyrophosphates, ferric salts, ferric polysaccharide complexes and ferrous salts.

The invention discloses a root-promoting liquid fertilizer, which is prepared from the following raw materials in parts by weight: 2-4 parts of ferrous sulfate, 2-3 parts of ammonium molybdate tetrahydrate, 1-3 parts of potassium pyrophosphate, 5-7 parts of short rhizome of spathiphyllum, 6-9 parts of castor cake, 15-20 parts of bran, 5-8 parts of potassium sulfate, 7-10 parts of urea, 2-4 parts of matrine, 2-3 parts of zinc sulphate heptahydrate, 0.1-0.2 part of 6-benzylaminopurine, 0.1-0.2 part of triiodobenzoic acid, 4-8 parts of rose, 10-14 parts of brewer's grains and 8-12 parts of an ingredient. Due to the addition of the 6-benzylaminopurine, the triiodobenzoic acid and the like, the liquid fertilizer disclosed by the invention has the function of adjusting the raw materials with a crop growth function, and is capable of effectively promoting rooting and formation of flower buds of the crops; the nutrient components of a microelement fertilizer can be increased by extract liquids such as chaff and brewer's grains; and the root-promoting liquid fertilizer is convenient to use, and obvious in yield-increasing effect, and has a good application prospect.

The invention provides a genetically engineered bacterium for high-yielding L-valine. A construction method of the genetically engineered bacterium comprises the steps that starting from an escherichia coli W3110, an acetolactate synthase gene alsS of a bacillus subtilis is integrated on a genome of the escherichia coli W3110 and subjected to high expression; an escherichia coli ppGpp 3'-pyrophosphoric acid hydrolytic enzyme mutant R290E/K292D gene spoT is integrated on the genome of the escherichia coli W3110 and subjected to high expression; genes of frdA, frdB, frdC and frdD of four subunits of a lactic dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB and fumaric reductase on the genome of the escherichia coli W3110 are knocked out; a branched chain amino acid transaminasegene ilvE of the escherichia coli is replaced with leucine dehydrogenase gene bcd of the bacillus subtilis; and an acetyl-hydroxyl acid isomerized reductase gene ilvC of the escherichia coli is replaced with an encoding gene of a mutant L67E/R68F/K75E. According to the genetically engineered bacterium for the high-yielding L-valine, an L-valine fermentation method is further modified. Double-phasedissolved oxygen control is adopted, and the L-valine yield and the saccharic acid conversion rate are improved.

Provided is a thermoplastic resin composition comprising 1 to 100 parts by weight of a flame retardant (B) containing any one or more flame retardants (B-1) selected from melamine phosphate, melamine pyrophosphate, and melamine polyphosphate and any one or more flame retardants (B-2) selected from piperazine phosphate, piperazine pyrophosphate, and piperazine polyphosphate, based on 100 parts by weight of a thermoplastic resin (A) comprising polylactide resin, wherein, according to transmission electronic microscopy, the major axis of the largest particle of the flame retardant (B) in the composition is not more than 10 μm, and the ratio of the number of particles of the flame retardant (B) having a major axis of not more than 3 μm relative to the number of particles of the flame retardant (B) per an area of 1,000 μm² is 70% or more.