The invention discloses a total parenteral nutrient solution preparation device, and relates to the technical field of parenteral nutrient solution preparation. The device comprises a quantifying mechanism, a preparing mechanism, a stirring mechanism, a fixing mechanism and a shaking-up mechanism. The bolt is fixedly arranged on the inner surface of the clamping groove and penetrates through the inserting groove, and the outer surface of the threaded shaft is in threaded connection with the end cover, wherein the threaded shaft is rotatably arranged above the piston, the driving wheel is arranged on the outer surface of the sleeve shaft, the elastic piece is arranged on the outer surface of the pressing plate, and the transmission block is arranged at one end of the transmission rod. By arranging the quantifying mechanism, the preparing mechanism, the stirring mechanism, the fixing mechanism and the shaking-up mechanism, medicine can be conveniently and quantitatively injected, the medicine can be conveniently injected, stirring and uniform mixing are convenient, a nutrition bag can be conveniently fixed, a nutrient solution can be automatically shaken up, and the labor intensity of workers is reduced. Therefore, preparation operation of the nutrient solution is greatly facilitated, the preparation efficiency can be improved to a certain extent, and the device is suitable for large-scale preparation.