6 results about "Eicosapentaenoic acid" patented technology

The present application belongs to the field of microalgae biotechnology, microalgae culture regulation and high-value lipid bio-manufacturing, and specifically relates to a method for improving microalgae biomass and eicosapentaenoic acid (EPA) yield by regulating amino acids, which takes microalgae capable of synthesizing EPA as a culture object, adds exogenous amino acids or amino acid combinations to the culture system under non-stress culture conditions containing sufficient inorganic nitrogen source, regulates photosynthesis, growth metabolism and lipid synthesis process of the microalgae, and simultaneously improves microalgae biomass and EPA volumetric yield; by adding specific exogenous amino acids or amino acid combinations under the condition of sufficient nutrition culture, the present application realizes synchronous promotion of microalgae growth and EPA accumulation. Different from traditional methods of relying on stress such as nitrogen deficiency to induce lipid accumulation, the present application can improve microalgae biomass, photosynthetic performance, pigment yield and EPA volumetric yield without significantly inhibiting cell growth and photosynthetic activity.

The invention discloses a method for improving killing activity of NK cells, and belongs to the technical field of cellular immunity. Carrying out segmented in-vitro culture on the separated NK cells by adopting a specific basic induction culture medium, an activation induction culture medium and an amplification culture medium, wherein recombinant human insulin and sodium selenite are added into the basic induction culture medium; ganoderma lucidum polysaccharides, eicosapentaenoic acid, IL-15 and IL-18 are added into the activation and induction culture medium. Salidroside, tea polyphenol, low molecular weight chitosan, IL-2 (interleukin-2), IL-21 (interleukin-21) and SCF (mesenchymal stem cells) are added into the amplification culture medium. According to the method, the killing ability of the NK cells obtained through in-vitro culture on tumor cells and the in-vitro proliferation effect of the NK cells are remarkably improved, the problems that in the prior art, the in-vitro proliferation efficiency of the NK cells is low, and cell activity is difficult to consider are solved, and a foundation is laid for clinical transformation of NK cell immunotherapy.

The invention discloses a culture method of umbilical cord mesenchymal stem cells for treating cognitive impairment, and belongs to the technical field of cell culture. The culture method specifically comprises the following steps: (1) preparing a special culture medium; (2) umbilical cord pretreatment; and (3) cell culture. The traditional Chinese medicine extract (astragaloside), the B cell depleting agent (rituximab and ibrutinib) and the metabolism regulator (eicosapentaenoic acid) are jointly applied to the UC-MSCs in-vitro culture process, the multiplication capacity, the anti-inflammatory characteristic and the neural restoration function of the UC-MSCs can be synergistically enhanced, and therefore the cognitive disorder treatment efficiency of the UC-MSCs is remarkably improved.

The invention belongs to the technical field of microorganisms, and discloses a high-yield EPA schizochytrium limacinum strain based on normal-pressure and room-temperature plasma mutagenesis and a preparation method and application thereof.The preparation method comprises the following steps that 1, wild schizochytrium limacinum HX308 growing in an exponential period is selected, and a NaCl salt solution with the mass concentration of 2% is used for preparing a bacterial suspension; (2) carrying out mutagenesis on the bacterial suspension in an ARTP mutagenesis instrument; (3) uniformly coating a screened solid culture medium with the mutagenized strain, and culturing in an incubator at 28 DEG C; (4) selecting strains with the fatality rate of 85% on the screening plate for subsequent fermentation culture, and detecting the yield of EPA (Eicosapentaenoic Acid); and (5) determining the EPA content of the mutant strain through a gas chromatographic method, and screening out the schizochytrium limacinum strain with high yield of EPA. The EPA high-yield strain is finally obtained, the yield reaches 5.72 g / L and is 6.09 times that of a wild type with the yield being 0.94 g / L, and the economic effect is remarkable.