The invention relates to a
straw-degrading composite microbial inoculum which is prepared by mixing a microbial inoculum No.I and a microbial inoculum No.II, wherein the microbial inoculum No.I comprises phanerochete
chrysosporium, cacillus and streptomycete; and the microbial inoculum No.II comprises
trichoderma,
penicillium,
aspergillus and actinomycete. The application method of the high-efficiency
straw-degrading composite microbial inoculum in pretreatment of
ethanol production comprises the following steps: soaking crushed straws in
ammonia water to carry out cleaning treatment; adding a
nutrient solution to prepare a
fermentation substrate; spraying the high-efficiency
straw-degrading composite microbial inoculum, and carrying out
fermentation treatment for two days; and filtering,and washing with water to neutral, thus obtaining the pretreatment product for
ethanol production. The invention has the following advantages: the treatment of soaking straws in
ammonia water is beneficial to removing
lignin, increasing the
enzyme contact area of celluloses and hemicelluloses and improving the pretreatment efficiency; and by combining the microbial inoculum and the
fermentation process to degrade straws, different microbes can secrete complementary enzymes in the
degradation process, so that the substrate can be thoroughly degraded, thereby improving the
biomass resource utilization rate. Besides, the invention is simple in process and convenient to implement, and can not do harm to the environment.