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Straw-degrading composite microbial inoculum and application thereof in pretreatment of ethanol production

A compound bacterial agent and pretreatment technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of large environmental impact, low degradation efficiency, and high operation cost, and achieve simple equipment, efficient pretreatment, and improved efficiency. Effect

Active Publication Date: 2012-04-25
AOWEI TIANJIN ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical and chemical methods have high requirements on equipment, high energy consumption, and have the disadvantages of high operating cost and great impact on the environment; in contrast, biological pretreatment has low energy consumption and is environmentally friendly, but has degradation efficiency low disadvantage

Method used

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  • Straw-degrading composite microbial inoculum and application thereof in pretreatment of ethanol production
  • Straw-degrading composite microbial inoculum and application thereof in pretreatment of ethanol production
  • Straw-degrading composite microbial inoculum and application thereof in pretreatment of ethanol production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for applying straw high-efficiency degradation compound bacterial agent in ethanol production pretreatment, comprising the following steps:

[0028] 1) The rice straw is crushed to a particle size of 0.8 cm by crushing equipment to form straw powder;

[0029] 2) soak the straw powder in 0.1% ammonia solution for purification treatment for 48 hours, take it out and squeeze until no water flows out;

[0030] 3) Add 40% of its quality nutrient solution to the purified straw to make a fermentation substrate; spray lignin-degrading bacteria and cellulose-degrading bacteria liquid into the fermentation substrate, and the quality of the bacterial liquid and the fermentation substrate The ratio is 1:3, and the bacterial concentration in the culture medium is 1×10 7 cfu / ml;

[0031] 4) After 2 days of fermentation treatment to obtain a fermentation product, the fermentation product was filtered and washed until neutral to obtain a pretreated product.

[0032] In this...

Embodiment 2

[0037] A method for applying straw high-efficiency degradation compound bacterial agent in ethanol production pretreatment, comprising the following steps:

[0038] 1) The rice straw is crushed to a particle size of 0.8 cm by crushing equipment to form straw powder;

[0039] 2) Soak the straw powder in 0.5% ammonia solution for purification treatment for 36 hours, take it out and squeeze until no water flows out;

[0040] 3) Add 30% of its mass nutrient solution to the purified straw to make a fermentation substrate; spray lignin-degrading bacteria and cellulose-degrading bacteria liquid into the fermentation substrate, and the quality of the bacterial liquid and the fermentation substrate The ratio is 1:5, and the bacterial cell concentration in the culture medium is 7×10 7 cfu / ml;

[0041] 4) After 2 days of fermentation treatment to obtain a fermentation product, the fermentation product was filtered and washed until neutral to obtain a pretreated product.

[0042] In th...

Embodiment 3

[0047] A method for applying straw high-efficiency degradation compound bacterial agent in ethanol production pretreatment, comprising the following steps:

[0048] 1) The rice straw is crushed to a particle size of 0.5 cm by crushing equipment to form straw powder;

[0049] 2) Soak the straw powder in 1.0% ammonia solution for purification treatment for 24 hours, take it out and squeeze it until no water flows out;

[0050] 3) Add 25% of its quality nutrient solution to the purified straw to make a fermentation substrate; spray lignin-degrading bacteria and cellulose-degrading bacteria liquid into the fermentation substrate, and the quality of the bacterial liquid and the fermentation substrate The ratio is 1:7, and the bacterial concentration in the culture medium is 7×10 8 cfu / ml;

[0051] 4) After 2 days of fermentation treatment to obtain a fermentation product, the fermentation product was filtered and washed until neutral to obtain a pretreated product.

[0052] In t...

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Abstract

The invention relates to a straw-degrading composite microbial inoculum which is prepared by mixing a microbial inoculum No.I and a microbial inoculum No.II, wherein the microbial inoculum No.I comprises phanerochete chrysosporium, cacillus and streptomycete; and the microbial inoculum No.II comprises trichoderma, penicillium, aspergillus and actinomycete. The application method of the high-efficiency straw-degrading composite microbial inoculum in pretreatment of ethanol production comprises the following steps: soaking crushed straws in ammonia water to carry out cleaning treatment; adding a nutrient solution to prepare a fermentation substrate; spraying the high-efficiency straw-degrading composite microbial inoculum, and carrying out fermentation treatment for two days; and filtering,and washing with water to neutral, thus obtaining the pretreatment product for ethanol production. The invention has the following advantages: the treatment of soaking straws in ammonia water is beneficial to removing lignin, increasing the enzyme contact area of celluloses and hemicelluloses and improving the pretreatment efficiency; and by combining the microbial inoculum and the fermentation process to degrade straws, different microbes can secrete complementary enzymes in the degradation process, so that the substrate can be thoroughly degraded, thereby improving the biomass resource utilization rate. Besides, the invention is simple in process and convenient to implement, and can not do harm to the environment.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation and environmental protection, in particular to a straw-degrading composite bacterial agent and its application in ethanol production pretreatment Background technique [0002] More than 200 years after the industrial revolution, human society has experienced unprecedented development, and social productivity and technological level have been greatly improved. These resource reserves are limited and belong to non-renewable energy, and the burning of fossil fuels is affecting global climate change and seriously threatening the living environment of human beings. Given that energy and environmental issues have become the focus of global attention, it is necessary to develop clean and renewable energy has become an urgent issue. Microbial fermentation to produce fuel ethanol as a renewable energy has attracted people's attention. [0003] At present, large-scale production of fuel eth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12P39/00C12P7/10C12R1/07C12R1/465C12R1/885C12R1/80C12R1/66C12R1/645
CPCY02E50/16Y02E50/10
Inventor 刘乐鞠美庭李维尊王雁南江洋
Owner AOWEI TIANJIN ENVIRONMENTAL PROTECTION TECH
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