The invention relates to a 
straw-degrading composite microbial inoculum which is prepared by mixing a microbial inoculum No.I and a microbial inoculum No.II, wherein the microbial inoculum No.I comprises phanerochete 
chrysosporium, cacillus and streptomycete; and the microbial inoculum No.II comprises 
trichoderma, 
penicillium, 
aspergillus and actinomycete. The application method of the high-efficiency 
straw-degrading composite microbial inoculum in pretreatment of 
ethanol production comprises the following steps: soaking crushed straws in 
ammonia water to carry out cleaning treatment; adding a 
nutrient solution to prepare a 
fermentation substrate; spraying the high-efficiency 
straw-degrading composite microbial inoculum, and carrying out 
fermentation treatment for two days; and filtering,and washing with water to neutral, thus obtaining the pretreatment product for 
ethanol production. The invention has the following advantages: the treatment of soaking straws in 
ammonia water is beneficial to removing 
lignin, increasing the 
enzyme contact area of celluloses and hemicelluloses and improving the pretreatment efficiency; and by combining the microbial inoculum and the 
fermentation process to degrade straws, different microbes can secrete complementary enzymes in the 
degradation process, so that the substrate can be thoroughly degraded, thereby improving the 
biomass resource utilization rate. Besides, the invention is simple in process and convenient to implement, and can not do harm to the environment.