9573results about How to "Improve purification effect" patented technology

The present invention relates to novel domain exchanged, bivalent, bispecific antibodies, their manufacture and use.

In certain aspects, the present invention provides compositions and methods for promoting bone growth and increasing bone density.

Novel and efficient syntheses create novel piperazinone intermediates which facilitate the production and use of PNAs. Such syntheses and the products enhance the feasibility of a system which permits the rapid identification of PNA oligomers useful as therapeutics, diagnostics and/or gene characterization tools. A first component of the system is a universal PNA library that most preferably incorporates one or more universal nucleotide bases into carefully selected positions within each oligomer species thereby providing the library with the screening ability of a much larger library. The second component of the system is a high throughput screening system that includes a number of assays designed to provide information on the binding activities of the different PNAs to a target nucleotide sequence (generally, a DNA or RNA sequence). The third component is a software system especially designed to provide rapid analysis of the data collected from the high throughput screening system and to determine therefrom the sequence base identities and sequence lengths of PNA oligomers most likely to bind to and appropriately affect the target molecule.

The invention provides a thick coating material capable of purifying air and a preparation method thereof, belonging to the building material field. An internal wall coating decorative material comprises 5 to 40 weight portions of a porous absorbing material and 10 to 40 weight portions of a composite photocatalysis material in every 100 weight portion of the paint. In the preparation method, the coating material, is formed by compounding a mineral material with the nanometer photocatalysis material to obtain a high activity purifying material, matching the high activity purifying material with other nanometer materials and the porous absorbing material, and then adding an inorganic filling material, a skeletal material and other addition agents, can remove harmful gases such as formaldehyde and toluol in air and has the environmental protection function. The paint has good effect for removing formaldehyde, is effective continuously, has certain thickness when the coating layer is used, has simple construction, and beautiful and elegant patterns, is well-bedded, is strong in the three-dimension effect and has good decorative effect.

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

A method of assay for target polynucleotides includes steps of isolating target polynucleotides from extraneous non-target polynucleotides, debris, and impurities and amplifying the target polynucleotide.

In certain aspects, the present invention provides compositions and methods for decreasing FSH levels in a patient. The patient may, for example, be diagnosed with an FSH-related disorder or desire to delay or inhibit germ cell maturation.

The present invention provides a linker preferably used when constructing an mRNA/cDNA-puromycin-protein conjugate used in an in vitro virus method, and an mRNA/cDNA-puromycin-protein conjugate constructed using that linker. More specifically, the present invention provides a linker for ligating mRNA and puromycin or a puromycin-like compound to construct an mRNA/cDNA-puromycin-protein conjugate, the linker comprising a single-stranded RNA as a main backbone, and having, in this main backbone, a solid phase binding site for binding an mRNA-puromycin-protein conjugate to a solid phase site, and a pair of cleavage sites provided at locations surrounding the solid phase binding site; an mRNA-puromycin-protein conjugate constructed using this linker; an mRNA bead or an mRNA chip comprising this conjugate; a protein chip produced from this mRNA chip; and a diagnostic kit using the mRNA bead or the mRNA chip.

The invention relates to methods of increasing the titre of a protein of interest in a cell as well as the improved production and purification of optimised biomolecules, one component of which is the domain C_H3. A frequently observed effect in biomolecules is the cleaving of the C-terminal amino acid(s), e.g. the C-terminal lysine. The usually incomplete processing of the heavy chain of antibodies for example leads to product heterogeneity. To prevent this product heterogeneity the corresponding codon of the C-terminal lysine of the heavy antibody chain has been deleted by recombinant DNA technology. These optimised antibodies lead to a product titre which is higher than in the wild-type. In addition, they prove advantageous during purification by having better elution characteristics as a result of the reduced charge heterogeneity.

A cell surface molecule that is expressed specifically in thymocytes, lymphocytes activated by ConA-stimulation, and peripheral blood lymphocytes. This molecule is involved in signal transmission of the secondary signal (costimulatory signal) essential for the activation of lymphocytes such as T cells and regulates functions of activated lymphocytes such as activated T cells. Disclosed are an antibody or a portion thereof, which binds to a polypeptide of the cell surface molecule, a polypeptide fragment thereof, or a fusion polypeptide comprising the fragment; a cell secreting the antibody or its portion; a pharmaceutical composition comprising the antibody; and methods of using the compositions for therapeutic, diagnostic and/or experimental purpose.

A method for reducing and re-using waste heat and water resulting from thermal hydrocarbon recovery operations involving accessing a hot water stream produced in a thermal hydrocarbon recovery operation; vaporizing water from the water stream by applying a vacuum, thereby producing water vapor; and condensing the water vapor to produce high quality water. A system is described including a hot water intake interfacing with a hot water stream from a thermal hydrocarbon recovery operation; a vaporization module receiving the hot water stream from the hot water intake, comprising a vacuum chamber in which a vacuum is applied to produce water vapor from the hot water stream; a condensation module in which water vapor produced in the vaporization module is condensed to form high quality water; and a water outlet for releasing the high quality water from the condensation module for re-use within the thermal hydrocarbon recovery operation.

The invention relates to a method for detecting the residual quantity of pesticides in a vegetable sample by utilizing a liquid chromatogram-high resolution mass spectrometer, which belongs to the technical field of a food safety detecting. The method provided by the invention comprises the following steps: 1) extracting the residual acetonitrile in the sample; 2) carrying out the dispersion solid-phase extraction and purification on a sample extraction solution; 3) detecting by utilizing the liquid chromatogram-high resolution mass spectrometer; and 4) analyzing the result. In the method provided by the invention, the quality accuracy of 27 pesticides is less than 1ppm; the detection limit is 0.01-0.05mg/kg; the addition recovery rate is 80-100%; and the relative standard deviation is less than 15%.

An exhaust purification apparatus for an engine has a first casing (17) that is interposed in an exhaust path (13); a second casing (23) that is set downstream of the first casing (17) and contains an exhaust purification device (24); a connecting pipe (22, 41, 51) that connects the first casing and the second casing (23) to each other and includes an insertion portion that is inserted in the first casing (17); and an injection nozzle (27, 52) that has a tip end inserted in the connecting pipe (22, 41, 51) and injects an auxiliary agent from the tip end. The insertion portion of the connecting pipe (22, 41, 51) is provided with a plurality of through-holes (22a, 41a, 41b, 51a) connecting the inside and the outside of the connecting pipe (22, 41, 51), so that the exhaust gas contained in the first casing (17) is introduced into the connecting pipe (22, 41, 51) through the through-holes and guided towards the second casing (23).

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

The present invention discloses a formaldehyde purification diatom mud decoration material and a preparation method thereof. Specifically the formaldehyde purification diatom mud decoration material comprises, by mass, 10.0-30.0 parts of refined diatomite, 20.0-50.0 parts of a powdery filler, 2.0-5.0 parts of redispersible glue powder, 20.0-40.0 parts of a gel material, 0.5-1.0 part of a dispersion medium, 3.0-8.0 parts of nanometer titanium dioxide, 1.0-4.0 parts of a modifier, 3.0-5.0 parts of anti-cracking fibers, and 20-80 parts of water. According to the present invention, with the formaldehyde purification diatom mud decoration material, the formaldehyde purification efficiency can be effectively increased, the purification persistence can be significantly improved, the defects of physical adsorption effect losing caused by saturation, and the like are avoided, and the purification speed can be accelerated; and the formaldehyde purification diatom mud decoration material of the present invention further has characteristics of good thixotropy, appropriate modeling, water resistance, alkali resistance, no powdering, no cracking and other beneficial properties, and concurrently meets process requirements of mechanical spraying, brushing coating, roller coating and the like.

Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.

A protein expression vector containing (a) a nucleotide sequence encoding an IgG(κ) or a trypsin secretory signal peptide, (b) a nucleotide sequence encoding a polyhistidine amino acid sequence, (c) a nucleotide sequence encoding an amino acid sequence comprising amino acid residues 36–40 of SEQ ID NO:19 (Asp-Asp-Asp-Asp-Lys), which is cleavable by an enterokinase, and (d) a cloning site into which a nucleotide sequence encoding a target protein can be inserted, wherein (a), (b), (c) and (d) are assembled within the vector in the order recited.

The present invention discloses a fluid purification and disinfection system, which includes a housing, an ultraviolet lamp and a photocatalytic oxidation device. Said housing therein is an enclosed case that is fitted with an inlet and an outlet. Furthermore, said ultraviolet lamp is mounted inside the housing. Said photocatalytic oxidation device is a disinfection core coated with photocatalyst. Said disinfection core is installed around said ultraviolet lamp, and is fixed onto said housing. Said photocatalyst therein is titanium dioxide. The working principle of the fluid purification and disinfection device in accordance with the present invention is to utilize ultraviolet light to irradiate the titanium dioxide-coated surface of the photocatalytic oxidation device to generate the photocatalytic oxidation process. As a result, Escherichia coli, Vibriocholerae and pathogenic organisms that contact the surface of photocatalytic oxidation device can be quickly killed, and contaminants in the fluid can be eliminated. By this way, water or fluid that flows through said disinfection device is disinfected and purified.

Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.

Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.

In certain aspects, the present invention provides methods for dosing a patient with an ActRIIb antagonist and methods for managing patients treated with an ActRIIb anatagonist. In certain aspects, the methods involve measuring one or more hematologic parameters in a patient.