920 results about "Red blood cell" patented technology

The invention relates to the technical field of target detection, and discloses a blood cell detection method based on improved YOLOv8. According to the method, a convolutional attention module (CBAM) is introduced, so that the detection performance of the model on a shielded small target is improved; a new loss function S-MPDIOU is set, so that the accuracy and robustness of the loss function are further improved; the multi-scale feature fusion technology is adopted to better capture blood cell information of different sizes, so that the model can more accurately identify small target platelets; the recall rate is improved by improving a non-maximum suppression (NMS) algorithm, and particularly under the condition of dense blood cells or complex forms, the improved NMS algorithm can more effectively solve the overlapping problem and reduce false detection and missing detection. Through testing, the model can accurately identify and position different types of blood cells such as red blood cells, white blood cells and blood platelets in an image, and a new effective method is provided for blood cell detection.

The invention provides a method for determining total folic acid in red blood cells by liquid chromatography-tandem mass spectrometry, which comprises the following steps: firstly, detecting the concentrations of two folic acid metabolites, namely 5-methyltetrahydrofolic acid and 5, 10-methylenetetrahydrofolic acid in the red blood cells by liquid chromatography-tandem mass spectrometry; and then the measured concentration is substituted into a formula (C red blood cell total folic acid = C red blood cell 5-methyltetrahydrofolic acid + a * C red blood cell 5, 10-methylenetetrahydrofolic acid, correction factor a = 3), the red blood cell total folic acid concentration is calculated, and the formula is obtained by correcting a detection result of a folic acid protein binding method. In conclusion, the blank that the total folic acid in the red blood cells cannot be detected by a liquid chromatography-tandem mass spectrometry method is filled, and the detection method is simple in step and accurate in result.

A centrifuge having a rotor attachment for preparing a small-format blood collection tube after blood collection for handling and analysis. The rotor attachment may include two or more small-container holding orifices designed to hold each container in a plane that is normal (or near-normal) to an axis of rotation during centrifuging. That is, the angle at which each small-format container is held during a centrifugation procedure is low (e.g., zero or near-zero). Having a rotor attachment that imparts a small angle or near-zero angle during centrifugation results is solutions being separated in layers that are aligned normal to a vertical axis of the small-format container. This separation into layers in the small-format container is more robust during handling and transport as a mid-level gel exhibits strong cohesion to the inner walls of the small-format containers, thereby maintaining separation between red blood cells as collected and a resident serum.

The invention discloses a blood fat test card based on a photochemical principle, and belongs to the technical field of in-vitro detection. The blood fat detection card comprises a card body, the card body is provided with a sample adding port, a correction area and at least one test area, the sample adding port is respectively communicated with the correction area and the test area, and the correction area and the test area are respectively provided with an air hole communicated with the outside; the correction area is provided with a reaction film and a hemolytic agent; and the test area is provided with an enzyme layer and an interference removing film corresponding to a to-be-detected item. The photochemical principle is adopted, the reflected light values of the test area and the correction area before and after the reaction are obtained, the luminosity value difference related to the initial concentration and hematocrit of the item to be detected is obtained, the concentration value of the item to be detected is corrected through the luminosity value difference related to the hematocrit, and therefore the corrected concentration value of the item to be detected is calculated. The detection result is more accurate and reliable, and the operation is simple.

The invention features polypeptides that include an extracellular ActRIIA variant. In some embodiments, a polypeptide of the invention includes an extracellular ActRIIA variant fused to an Fc domain monomer or moiety. The invention also features pharmaceutical compositions and methods of using the polypeptides to treat diseases and conditions involving low red blood cell levels, e.g., anemia or blood loss; fibrosis; or pulmonary hypertension.

The invention discloses a traditional Chinese medicine composition with the effects of benefiting qi, nourishing blood, invigorating the spleen and tonifying the kidney and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: cistanche, ginseng, American ginseng, astragalus membranaceus, medlar, rhizoma polygonati, oyster peptide, angelica sinensis, radix rehmanniae preparata, donkey-hide gelatin, fructus amomi, pericarpium citri reticulatae, hawthorn, folium cortex eucommiae, radix puerariae, liquorice and the like. The preparation method comprises the following steps: respectively crushing the medicinal materials, dissolving the colla corii asini, mixing, adding water, granulating and the like. The composition can be prepared into various dosage forms such as granules, capsules, tablets, oral liquid and the like. Experiments show that the traditional Chinese medicine composition can effectively improve the symptoms of deficiency of qi and blood (increasing erythrocyte count and hemoglobin content), weakness of spleen and stomach (increasing body weight and improving pepsin activity) and kidney deficiency (reducing creatinine and urea nitrogen content and improving SOD activity), and acute toxicity experiments show that the toxicity is slightly reversible under high dosage, and the safety is good under therapeutic dosage. The traditional Chinese medicine composition has potential application value in the aspect of preparing medicines for benefiting qi, nourishing blood, invigorating spleen and tonifying kidney.

The invention provides an anti-aging and whitening pyracantha fortuneana fruit extract prepared by adopting a supramolecular solvent and a preparation method of the pyracantha fortuneana fruit extract, and relates to the technical field of biological extraction. The preparation method comprises the following steps: by taking betaine as a hydrogen bond acceptor and 1, 3-propylene glycol as a hydrogen bond donor, mixing and self-assembling the betaine and the 1, 3-propylene glycol to form a supramolecular solvent; and the pyracantha fortuneana fruits are extracted by adopting the supramolecular solvent. The pyracantha fortuneana fruit active ingredients are extracted by adopting the supramolecular solvent which is harmless to the skin, so that the irritation of the solvent to the skin can be avoided, the supramolecular solvent can be specifically combined with the active ingredients in the pyracantha fortuneana fruit, and the purity and activity of the active ingredients are improved. The pyracantha fortuneana fruit extract extracted by adopting the supramolecular solvent has better oxidation reduction resistance; generation of non-enzymatic glycosylation intermediate products is inhibited, and saccharification and aging resistance are facilitated; the red blood cell oxidative damage is improved, and the effects of resisting oxidation and relieving are achieved; the whitening mask has an inhibiting effect on elastinase and tyrosinase, and can resist aging, whiten skin, relieve facial pigmentation and brighten the skin.

The invention discloses a blood component separation system and a control method, and belongs to the field of blood separation.The system comprises a centrifugal device, a blood supply pipeline, an air detector valve and a blood pump, a liquid outlet of the centrifugal device is connected with a separation pipeline, and the separation pipeline is sequentially connected with a first plasma bag and a second plasma bag; a centrifugal pressure sensor, a centrifugal high-speed camera and a red blood cell sensor; and the control center comprises a data acquisition module, a data analysis module and a centrifugal control module. The control method comprises steps S1 to S4. Dynamic intelligent regulation and control and automatic control of the blood component centrifugal separation system are achieved, the centrifugal effect is optimized through the image recognition technology, the image recognition technology is superior to the photoelectric recognition technology in the prior art, excessive centrifugation is avoided, meanwhile, the centrifugal quality is guaranteed, and the quality and activity of separated blood components are effectively guaranteed.

The invention features polypeptides that include an extracellular ActRIIB variant. In some embodiments, a polypeptide of the invention includes an extracellular ActRIIB variant fused to an Fc domain monomer or moiety. The invention also features pharmaceutical compositions and methods of using the polypeptides to treat diseases and conditions involving weakness and atrophy of muscles, bone damage, low red blood cell levels (e.g., anemia or blood loss), fibrosis, and / or pulmonary hypertension.

The embodiment of the invention provides a continuous blood oxygen monitoring system in extracorporeal circulation and a control method thereof. The continuous blood oxygen monitoring system comprises a vein sensor, an artery sensor, a blood flow sensor, an oxygen sensor, a carbon dioxide sensor, a controller and a display, the vein sensor monitors the vein blood oxygen saturation degree; the artery sensor monitors arterial oxygen saturation and hematocrit; the blood flow sensor monitors the blood flow of the artery pipeline; the oxygen sensor monitors the oxygen concentration of the oxygenator; the carbon dioxide sensor monitors the carbon dioxide concentration of the oxygenator; the display provides an interaction control, responds to a trigger operation acting on the interaction control, sends an interaction instruction to the controller, and updates information displayed in an interaction interface according to the control of the controller; the controller receives the interaction instruction and executes processing corresponding to the interaction instruction; the processing includes controlling display information of the display; the display information comprises information corresponding to the monitoring parameters. The scheme ensures the safety and effectiveness of the treatment process.

The present invention belongs to the field of biotechnology, and specifically relates to an antibody targeting CD47 and its application. The anti-human CD47 antibody provided by the present invention can effectively bind to recombinant CD47 protein, block the binding of CD47 to its biological ligand SIRPa, and effectively identify and bind to CD47 positive tumor cells. At the same time, the CD47 antibody does not cause significant hemagglutination reaction and red blood cell binding of human red blood cells at high concentrations.

There are provided polypeptides that include an Activin receptor type IIB (ActRIIB) ectodomain (ECD) variant. In some embodiments, a polypeptide of the disclosure includes an ActRIIB-ECD variant fused to an Fc domain moiety. The disclosure also provides pharmaceutical compositions and methods of using the polypeptides to treat diseases and conditions associated with TGFβ superfamily ligand signaling, such as pulmonary hypertension, fibrosis, muscle weakness and atrophy, metabolic disorders and / or cardiometabolic disease, bone damage, and / or low red blood cell levels (such as anemia).

The invention provides application of lactobacillus paracasei IOB413 and a metagen thereof in preparation of a medicine for improving radioactive hematopoietic injury, and relates to the field of microorganisms. The lactobacillus paracasei IOB413 is preserved in the China General Microbiological Culture Collection Center on June 29, 2018, and the preservation number of the lactobacillus paracasei IOB413 is CGMCC (China General Microbiological Culture Collection Center) No.16022. The lactobacillus paracasei IOB413 has the advantages that the lactobacillus paracasei IOB413 is preserved in the China General Microbiological Culture Collection Center; the lactobacillus paracasei IOB413 and the metaplasm thereof can improve the damage condition of immune organs, increase the number of lymphocytes, increase the number of red blood cells and blood platelets and recover the immune function and hematopoietic function of the organism, so that the radioactive hematopoietic damage is improved.

To improve measurement accuracy.A concentration measurement device includes: an absorbance measurement unit configured to measure absorbance in a plurality of different wavelength regions for a mixed liquid in which different liquids are mixed; and a control unit configured to correct a concentration of at least one liquid on the basis of absorbance in the plurality of different wavelength regions. The absorbance measurement unit measures absorbance in two wavelength regions. The absorbance measurement unit measures absorbance in a spectrum. Particles contained in one of the different liquids are different from particles contained in another of the different liquids. Each of the different liquids is a suspension or a solution. The mixed liquid includes a white blood cell suspension and a red blood cell suspension. The control unit calculates a mixing ratio of a liquid mixed in the mixed liquid, from absorbance in the plurality of different wavelength regions. The control unit corrects the absorbance on the basis of a mixing ratio of a liquid mixed in the mixed liquid.

The invention belongs to the technical field of medical equipment, and particularly relates to a blood growth factor extraction device and an information loading system.The blood growth factor extraction device comprises a box body used for connecting all components; the blood centrifugal assembly is arranged in the box body, and the blood centrifugal assembly is used for centrifugally removing red blood cells of the blood; the information field assembly is arranged in the box body, the information field assembly is connected with the blood centrifugal assembly, and the information field assembly is used for performing scalar wave action on blood in the blood centrifugal assembly and loading specific life factor information into plasma; the disinfection assembly is arranged in the box body, the disinfection assembly is connected with the blood centrifugal assembly, the disinfection assembly is used for disinfecting test tubes in the blood centrifugal assembly, and it is guaranteed that wound repair factors, blood platelets, growth factors and the like are more easily and sufficiently separated during separation and preparation.

The invention provides a red blood cell simulant as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) pretreatment: obtaining mammal whole blood and purifying to obtain red blood cells; (2) homogenization treatment: carrying out homogenization treatment on the red blood cells obtained in the step (1) by using a homogenization reagent; (3) cell repair treatment: carrying out cell repair treatment on the red blood cells obtained in the step (2) by using cell repair liquid; and (4) fixing treatment: fixing the red blood cells obtained in the step (3) by using a fixing agent. The preparation method disclosed by the invention is simple to operate and beneficial to large-scale production of products; the red blood cells in the prepared red blood cell simulant are good in homogeneity and stable in performance, the red blood cells in the red blood cell simulant are similar to red blood cells in human urine in shape, and the red blood cell simulant is used for preparing quality control substances for urine visible component analysis and can meet the requirements for accuracy and reliability of detection of the red blood cells in the urine.

The invention discloses a hemolysis rate detection method for metaraminol bitartrate injection, aims to overcome the defects in the prior art, and provides a hemolysis rate detection method capable of eliminating interference on hemolysis rate detection caused by reaction between metaraminol bitartrate injection and red blood cells. The method specifically comprises the following steps: adding metaraminol bitartrate injection into a red blood cell suspension, incubating, repeatedly cleaning uncrushed red blood cells until the supernatant is colorless and transparent, removing the supernatant, performing ultrasonic treatment on the residual red blood cells to crush all the red blood cells, performing centrifugal separation, taking the supernatant, and performing freeze-drying to obtain the red blood cell suspension. The absorbance value is measured on a spectrophotometer, and the hemolysis rate is calculated. Compared with the prior art, the invention provides the detection method based on the improved spectrophotometric method, through special treatment of unbroken red blood cells and combination of ultrasonication and cleaning steps, the problem of applicability of a traditional method in specific drugs is solved, and the accuracy and reliability of detection are greatly improved.

The invention relates to the technical field of blood flow velocity measurement, in particular to a three-dimensional laser Doppler blood flow velocity measurement system and method based on optical fiber sensing. The method comprises the following steps: adjusting the probe to enable a convergent point of five paths of incident light to irradiate a measured target point; red blood cells in a blood vessel move and interact with three groups of detection light through a target point to generate scattered light containing three wavelengths, a probe receives the scattered light, the scattered light is amplified and filtered and then output to three photoelectric detectors respectively, and the photoelectric detectors convert received optical signals into current signals; performing current-voltage conversion and analog-to-digital conversion on the current signal to obtain different digital signals, obtaining Doppler frequency shift through fast Fourier transform, and calculating velocity components in three independent directions based on the Doppler frequency shift; and obtaining the flow velocity of the blood in the blood vessel according to all the velocity components. The human body blood flow velocity can be accurately monitored.

The invention belongs to the technical field of cell engineering and biochemical detection, and particularly relates to an erythrocyte membrane with a complete structure and a preparation method and application thereof. The red blood cells are split by limiting the components of the release liquid, the conditions are mild, protein components such as hemoglobin in the red blood cells can be released, the cell attributes are removed, the integrity of the internal skeleton structure and the activity of the membrane surface antigen are reserved, the complete structure of the red cell membrane is kept, meanwhile, the key blood group antigen on the surface is reserved, and the effect is good. The problem of antigenicity reduction caused by erythrocyte membrane fragmentation is avoided. After the erythrocytes are lysed, the nanoparticles are coupled to the surface of the erythrocyte membrane, so that the agglutination reaction of the erythrocyte membrane under the induction of the antibody can be visualized. The engineering red blood cell biological membrane is preserved by adopting a fixed program, so that the damage of ice crystals to the engineering red blood cell biological membrane can be effectively avoided, the antigenicity of the membrane is not influenced, the membrane can be preserved for a long time at normal temperature, and the shelf life can be prolonged to two years or more.

The invention relates to the technical field of biological and medical diagnosis, in particular to a biomarker for predicting whether a patient suffering from pyotoxic myocardial injury has a short-term death risk or not and application of the biomarker, and the biomarker is the ratio of erythrocyte distribution width to albumin. According to the method, retrospective queue research design is adopted, two data sets, namely MIMIC-IV and eICU-CRD, are used, suppurative myocardial injury patients meeting conditions are included, covariants such as baseline features, laboratory inspection results and complication information of the patients are collected, the patients are divided into two groups according to RAR medians, the relation between RAR and short-term death of the patients is analyzed by adopting multiple statistical methods, and the accuracy of the short-term death of the patients is improved. A risk prediction model of short-term death of the patient suffering from the pyotoxic myocardial injury is constructed, so that a clinician can identify the patient suffering from the pyotoxic myocardial injury with high death risk in an early stage, a personalized treatment strategy can be formulated more accurately, the survival rate of the patient suffering from the pyotoxic myocardial injury is increased, and the method has a wide application prospect.

Fluid analyzation devices, methods, and systems are disclosed including an analyzation device comprising a sample vessel having an outer surface, a microchannel within the confines of the outer surface, a first port extending through the outer surface to the microchannel, and a second port extending through the outer surface to the microchannel; and an piezo transducer bonded to the outer surface of the sample vessel to form a monolithic structure, the piezo transducer configured to emit ultrasonic acoustic waves having a first frequency, a second frequency, and a third frequency into and / or to a blood sample within the microchannel, the first frequency configured to begin separation of red blood cells and plasma in the blood sample, the second frequency configured to complete separation of the red blood cells and plasma, and the third frequency configured to rupture cell walls of the blood cells producing a lysed blood sample.

The invention relates to the technical field of image processing, and provides an image-based blood flow velocity measurement method, system and device, a product and a medium, and the method comprises the steps: selecting a to-be-measured capillary; selecting a first position and a second position on the capillary to be measured, and measuring a horizontal azimuth difference and a vertical azimuth difference; respectively obtaining a first red blood cell image number and a second red blood cell image number, and calculating a red blood cell interval difference according to the first red blood cell image number and the second red blood cell image number; constructing a first motion equation and a second motion equation, and resolving through the first motion equation and the second motion equation to obtain a red blood cell velocity relationship; and constructing a differential relational expression of the red blood cell acceleration, substituting the red blood cell velocity relation and the second motion equation into the differential relational expression to obtain a target differential equation, and resolving the target differential equation to obtain the blood flow velocity. The blood flow velocity can be conveniently measured.

The invention discloses an intelligent negative pressure drainage system, and relates to the field of medical instruments, the intelligent negative pressure drainage system comprises a drainage module, a detection module, a cleaning module and a control module, the detection module detects pressure data in a drainage tube and a drainage liquid collector, flow data of drainage liquid and image data of the drainage liquid; the control module receives the image data in real time, judges the unit density of red blood cells in the drainage liquid, and gives an alarm to medical staff when the unit density is larger than a set threshold value; the storage module, the counting module and the display module are used for counting and displaying the red blood cell data in the drainage tube and the drainage liquid collector and the flow of the drainage liquid in real time; the control module receives the pressure data and the flow data in real time, judges whether the drainage tube is blocked or not and judges the position of a blockage object, and controls the cleaning head to reach the position of the blockage object for cleaning when blockage occurs. The operation condition of a drainage liquid system can be detected in real time, an alarm is given, postoperative possible bleeding is prompted, smooth drainage is guaranteed, and meanwhile rapid healing of a wound is promoted.

A blood processing device includes a pump system, a valve system, a separator and a controller configured to execute a plasma collection stage of a blood processing procedure by actuating the pump system and the valve system to convey blood from a blood source into the separator, actuating the separator to separate the blood into at least plasma and red blood cells, in a transfusion plasma collection step actuating the pump system and the valve system to convey a first volume of separated plasma out of the separator along a first flow path for transfusion plasma collection, and in a fractionation plasma collection step of the plasma collection stage, actuating the pump system and valve system to convey a second volume of the separated plasma out of the separator along a second flow path for fractionation plasma collection.

The invention relates to the field of enzyme and protein structures, in particular to fusion protease as well as a construction method and application thereof. The construction method comprises the following steps: carrying out gene recombination on the FpGalNAcDeAc and the FpGalNase, and carrying out prokaryotic expression and purification, so as to obtain the FpGalNAcDeAc-FpGalNase or the FpGalNase-FpGalNAcDeAc. The construction method comprises the following steps: carrying out gene recombination on the FpGalNAcDeAc and the FpGalNase; the invention describes that under the conditions of specific buffer solution, incubation time and enzyme concentration, A type red blood cells can be converted into universal O type red blood cells with maximum efficiency. The structure of galactosaminase is analyzed by using a cryoelectron microscope, and acetylgalactosamine deacetylase and galactosaminase are subjected to fusion expression, so that more efficient erythrocyte type conversion is carried out. The method can be used for rapidly, efficiently and safely carrying out type-A erythrocyte type conversion, and has the advantages of high protease yield, low working concentration, simple and convenient conversion operation, universal conditions, low cost and the like.

The present invention relates to systems and methods for optimizing plasma collection volume. A plasma withdrawal system and a method for operating a plasma withdrawal system are provided by which the volume / weight of the anticoagulated plasma collected is optimized. In one example, a nomogram is provided that utilizes the donor hematocrit to calculate the volume / weight of raw plasma within a plasma product having a maximum volume allowed by the FDA nomogram. In a plasma withdrawal process having multiple collection phases followed by a reinfusion cycle in which concentrated red blood cells are returned to the donor, the volume of plasma product to be collected is calculated prior to the start of each collection cycle to account for the increasing hematocrit of the donor, resulting in a greater total volume of plasma product to be collected during the plasma withdrawal process.

Disclosed in the present application are an HCT prediction method and system, a device, and a medium. Photons in a photon group are emitted into an extracorporeal circulation blood model so that the photons are reflected, scattered, or absorbed during movement. The extracorporeal circulation blood model is a model constructed on the basis of the thickness of an extracorporeal circulation tube, the thickness of the blood, and the distance from a light source generator to a detector. Light intensity data corresponding to photons reaching the detector are acquired. The acquired light intensity data are input into a prediction model to obtain a hematocrit (HCT) prediction value. The prediction model is a model constructed on the basis of a BP neural network and the relationship between light intensity and HCT. The prediction model combining simulation and neural network increases the data volume, achieves continuous non-invasive estimation of HCT by means of algorithm model optimization, and improves the accuracy of HCT prediction.

The invention features ALK2 antibodies and antigen binding fragments thereof (e.g., ALK2 binding fragments). The invention also features pharmaceutical compositions and methods of using the ALK2 antibodies or antigen binding fragments thereof to treat bone disease or damage, low red blood cell levels (e.g., anemia or blood loss), heterotopic ossification (e.g., heterotopic ossification resulting from fibrodysplasia ossificans progressiva), Sjogren's syndrome (e.g., dry eye associated with Sjogren's syndrome), multiple osteochondroma, diffuse intrinsic pontine glioma, posterior capsule opacification, or cardiac hypertrophy and / or cardiac fibrosis.

The invention provides autologous platelet-containing plasma gel and a preparation method and application thereof, and relates to the technical field of biological medicine and tissue engineering.The preparation method comprises the following steps that peripheral venous blood of a patient is extracted and added into a centrifugal tube mixed with a sodium citrate anticoagulant; putting the centrifugal tube into a centrifugal machine for centrifuging, and taking upper-layer plasma; wherein the upper-layer plasma refers to plasma between the upper-layer liquid level and the lower-layer red blood cell layer by 1mm; the centrifugal speed ranges from 1300 r / min to 1700 r / min; the centrifugation time is 3-5 min; under a sterile condition, preparing a calcium gluconate / thrombin mixed solution; and dropping the calcium gluconate / thrombin mixed solution into the upper plasma, mixing, and solidifying to obtain the autologous platelet-containing plasma gel. After one-time centrifugation, upper-layer full-layer plasma is extracted, the volume of the prepared gel is far larger than that of a traditional method, the utilization rate of whole blood platelets and fibrinogen is close to 100%, and regeneration and repair of large-area tissue wounds can be met.

[Problem] It is known that in wound healing, when pH in blood decreases, oxygen is released from hemoglobin, blood oxygen concentration rises, division of regenerated epithelial cells is promoted, and healing is promoted. Wound therapeutic agents such as creams or ointments are weakly acidic, and therefore, the described healing promoting effect can be expected; however, in being applied by the fingers, the wound therapeutic agent will need to be wiped off, and other adverse handling-related attributes are encountered, there being a risk presented of further damage to the wound when the agent is applied. The purpose of the invention is to provide a material for promoting the release of oxygen from red blood cells that has exceptional handling-related attributes and is capable of promoting division of regenerated epithelial cells and promoting healing of a wound. [Solution] A material for promoting the release of oxygen from red blood cells characterized in being a sheet-form material containing an acid-type carboxy-group-containing material, the amount of acid-type carboxy groups contained in the material being 0.05-10.0 mmol / g.