514 results about "Reagent strip" patented technology

The present invention concerns a reagent coating mass which can be used in slot-die-coating of flat support materials in the manufacturing processes of test strips. Advantageously, the reagent mass of the invention exhibits certain superior rheological properties such as viscosity, surface tension and thixotropy. The reagent mass is preferably used to coat thin, narrow and homogeneous stripes of reagent material onto flat web material.

Reagent test strips and methods for their use in the determination of the concentration of an analyte, e.g. glucose, in a physiological sample, are provided. The subject reagent test strips include one or more members of an analyte oxidation signal producing system and at least one hemolyzing agent. The subject reagent test strips and methods are particularly suited for use in the detection of blood glucose concentrations. Also provided are kits that include the subject test strips for use in practicing the subject methods.

The invention discloses a full-automatic immuno-fluorescence quantitative analysis device and an implementation method, belonging to the field of quantitative immuno-fluorescence analysis and detection. The full-automatic immuno-fluorescence quantitative analysis device comprises a supporting bottom plate, a reagentstrip storage and automatic loading module, a reactiondisc module, a detection module, a sample module, a sample adding module, a washing module and a control system, wherein the reagentstrip storage and automatic loading module, the reactiondisc module, the detection module, the sample module, the sample adding module and the washing module are arranged on the supporting bottom plate in sequence; the reagentstrip storage and automatic loading module provides a reagent strip for the reactiondisc module; the sample adding module is used for adding samples in the sample module into the reactiondisc module to carry out reaction, and after reaction is finished, the samples enter the detection module to be detected. The full-automatic immuno-fluorescence quantitative analysis device and the implementation method disclosed by the invention have the advantages that the difficulty that automation is hard to implement for an in-vitro diagnosis product is solved, the artificial error is reduced, the testing accuracy is improved and the detection efficiency is improved.

The invention relates to a reagent kit and the preparation method thereof which are used to detect cardiac muscle troponin I; the reagent kit provided by the invention comprises a reagent strip; the reagent strip contains cardiac muscle troponin I monoclonal antibody labeled by colloid gold, unlabeled cardiac muscle troponin I monoclonal antibody and the second antibody of anti-myocardial troponin I monoclonal antibody; the invention also provides the preparation method of the reagent kit of detection. The reagent kit of detection can detect cardiac muscle troponin I in blood sample drawn from human body swiftly, conveniently and delicately; the cardiac muscle troponin I can be used to diagnose myocardial infarction.

An assay device, analytical instrument and assay method for determining the presence or amount of an analyte in a fluid is disclosed. The device is a one-step lateral flow dry reagent immunoassay with one, two or more zones along a transverse axis of the device, each zone can contain or be preceded by diffusively or non-diffusively bound reagents. The invention measures an indicator in one or two test zones for each analyte to determine its presence or concentration. The signal reagent (indicator) may be a particle such as a colored latex or colloidal gold. The assay quantitation may be read by an instrument.

The invention discloses a full automatic chemiluminescence immune assay instrument. An upper needle plate group device, a photon detection device and a reagent strip propelling device are located above an upper plate; a specimen disc device and an incubation disc device are located below the upper plate; a dark room detection device is located in a region adjacent to the photon detection device and the reagent strip propelling device; a V-shaped notch slot is formed in a reagent putting station, corresponding to the incubation disc device, of the upper plate; both a puncture needle group device and an enhancing liquid needle group device in the upper plate needle group device are provided with a double needle structure which not only can move horizontally, but also can move vertically, and the other needle assemblies in the puncture needle group device and the enhancing liquid needle group device are stirring needle assemblies. As the station of the incubation disc device is of a reasonable layout structure and the double needle structure is combined in use, the automatic degree of the instrument is improved, and the whole instrument is simpler and more compact in structure; and moreover, the full automatic chemiluminescence immune assay instrument is low in cost and quite suitable for being popularized and generalized in medical units at home.

Disclosed is a reagent strip comprising test region(s) and reference region(s). The reference region(s) display a predetermined response to a range of possible concentrations of an analyte applied to the corresponding test region(s). Furthermore, the reference region(s) and the test region(s) are arranged on the reagent strip so as to facilitate analysis of a qualitative state of the analyte and optionally, calculation of a quantitative value of the analyte at point-of-collection. Also disclosed is a method of manufacturing such reagent strips, first involving printing the reference region(s) and the test region(s) in a continuous and alternating series on a test strip precursor substrate. The method also involves cutting the test strip precursor substrate perpendicularly to the printed columns to obtain one or more reagent strips. Also disclosed is a method encompassed in a mobile application for determining a qualitative state and optionally calculating a quantitative state of the analyte.

A detection device and a detection method are disclosed. The detection device comprises a sample inlet (132) that allows liquid samples flowing from a collection chamber (20) to a detection chamber (10), which comprises a through hole (135) that allows gases circulation between the detection chamber and the collection chamber, and a sheath (140) that prevents liquid samples from entering into the detection chamber from the collection chamber via the through hole. The detection chamber contains test reagent strips (120) to detect whether samples contain interested analytes. The detection device further comprises a protrusion (131) that enables the detection device standing tilted in the traverse direction. The detection method comprises collecting liquid samples as the first step, followed by standing the device tilted in its traverse direction through a protrusion support, and detecting whether samples contain the analytes.

The device comprises: a sample application zone, a reagent zone, a detection zone with positive and negative contrast zones and the combination zone for detected target, and a reagent strip. Wherein, the positive contrast zone comprises one or more material to show the first color on dried condition and the second color when on wet condition. If there is detected target in sample, the interactive action of the combination zone and contrast zone forms the legible symbols.

Belonging to medical diagnosis, especially the in vitro diagnosis field, the invention discloses a dry type biochemical immune mixed analysis system and a device thereof. The mixed analysis system includes a dry type biochemical and immune detection system able to individually or simultaneously complete biochemical and immune detection, and the system includes mixed type and parallel type two modes. The system device involved in the invention is combined by a support base plate, a reagent strip storage unit, a sample addition unit, a sample unit, a detection unit, and a circuit unit, and includes single reaction disk and double-reaction disk two forms. Specifically, the single reaction disk can complete biochemical and immune analysis mixedly, the double reaction disks correspond to biochemical and immune analysis procedures respectively, and the detection process of the two can be finished integratedly. According to the invention, between-sample pollution is effectively reduced, compared with single biochemical type or immune type, the system and the device greatly increase the number of detection items, and have the characteristics of fast detection speed and small blood collection amount, thus reducing the patient pain in the detection process.

Cholesterol from Low Density Lipoproteins (LDL-C) is measured directly with a test strip at room temperature using a reagent that takes advantage of the varying surface charge density on LDLs and non-LDLs to selectively make LDL-C available for testing.

The invention provides a sealing agent composition used for an immunochromatography reagent strip, which comprises protein, protein protective agent, surface active agent and first buffer solution, wherein the protein is casein, casein treatment solution, methylation bovine serum albumin (BSA) and human serum albumin; and the protein protective agent is saccharide. The sealing agent composition provided by the invention is capable of effectively reducing the background of the immunochromatography reagent strip, and is long in service life and high in detection sensitivity. The invention also provides the immunochromatography reagent strip.

The invention discloses a test strip for detecting HIV antibodies in spittle and a preparation method thereof. The test strip comprises a sample pad, a fiberglass film, a nitrocellulose film and absorbent paper, wherein the fiberglass film is tightly connected with one end of the sample pad and contains colloidal gold particle markers; the nitrocellulose film is tightly connected with the other end of the fiberglass film; the absorbent paper is tightly connected with the other end of the nitrocellulose film; the sample pad, the fiberglass film, the nitrocellulose film and the absorbent paper are all arranged on a base plate; the nitrocellulose film comprises a detection area coated with HIV recombinant antigens and a control area coated with goat anti rabbit antibodies; and the colloidal gold particle marker comprises a colloidal gold particle-avidin-biotin-antihuman IgG antibody composite micro-signal amplification system, and colloidal gold marking rabbie IgG antibodies. Due to the adoption of the avidin-biotin micro-signal amplification system, the test strip amplifies signals of the target antibodies, improves detection sensitivity and avoids false negative or detection omission caused by too weak signals.

The invention relates to the field of the emulsion method immune chromatography, and particularly relates to a detection kit of a helicobacter pylori emulsion method. The detection kit of the helicobacter pylori emulsion method comprises a reagent strip, wherein the reagent strip comprises a substrate, filter sample paper, a sensitized emulsion polyester film, an immune cellulose nitrate film and absorbent paper, wherein the filter sample paper, the sensitized emulsion polyester film, the immune cellulose nitrate film, and the absorbent paper are orderly connected end to end, and are fixed on the substrate, the immune cellulose nitrate film is covered with helicobacter pylori resisting urea enzyme antibodies I marked by colorful emulsion particles, and the immune cellulose nitrate film is provided with a detection line coated with helicobacter pylori resisting urea enzyme antibodies II and a quality control line coated by goat anti-rabbit serum IgG. The detection kit of the helicobacter pylori emulsion method, which is provided by the invention, has the advantages of convenience in detection, stability in detection results and cost conservation.

The invention discloses an immune chromatography fluorescence reagent strip for detecting cardiac troponin and a preparation method thereof, belonging to the field of clinical medicine detection. The reagent strip comprises a baseboard, a sample mat, a tracer particle combining mat, a nitrocellulose membrane and a water absorption mat; the sample mat, the tracer particle combining mat, the nitrocellulose membrane and the water absorption mat are orderly connected and fixed on the baseboard in the horizontal direction. The tracer particle combining mat is provided with the cTnI monoclonal antibody-fluorescence particle compound adsorbed layer covered on the surface of the nanocrystalline metal particle; the nitrocellulose membrane is provided with the detection line composed of the cTnI monoclonal antibody or polyclonal antibody capture molecule and the quality control line composed of the rabbit antimouse IgG antibody. The immune chromatography fluorescence reagent strip for detecting cardiac troponin is short in detection time, high in specificity and sensitivity and more accurate in detection result by improving the fluorescence intensity through the nanocrystalline metal particle.

The invention relates to an optical analysis reading device, which comprises at least one light source capable of being incident to a reagent strip, wherein the reagent strip comprises a first area, a second area and a third area which are spatially separated; the light source comprises three light-emitting diodes corresponding to the first area, the second area and the third area on the reagent strip respectively; and the device also comprises an optical detector capable of receiving reflected light of the three areas on the reagent strip. The device has low manufacturing cost, is easy to produce, has simple operation and intuitive display result, breaks through the conventional combination of two optical detectors and three light sources, optimizes the configuration of the optical detector, and achieves good effect.

The invention provides a fluorescent quantitative detection instrument. The instrument comprises an excitation light source module, a photoelectric conversion module, a control analysis module and a software system, wherein the excitation light source module comprises a first laser module component and a second laser module component; the first laser module component is used for transmitting an excitation beam A to a detection region on a reagent strip; the second laser module component is used for transmitting an excitation beam B to a coding region on the reagent strip; the photoelectric conversion module is used for receiving a reflected fluorescent signal C and a laser signal D and carrying out photoelectric signal conversion; the control analysis module comprises a main circuit, a motor and a display screen, and is used for processing received electric signals output by the photoelectric conversion module; the fluorescent quantitative detection instrument is provided with a reagent strip slot for placing a reagent strip and an ID card slot for placing a scaler chip ID card; and the motor is used for driving the reagent strip to move. The fluorescent quantitative detection instrument has the following beneficial effect: the specially designed optical system and compensation circuit are adopted, so the fluorescent quantitative detection instrument has higher sensitivity and accuracy in immunofluorescent quantitative detection.

The invention provides a homocysteine dry chemical detection strip and a preparation method thereof. The invention aims to provide a detection strip and a preparation method thereof for fast semiquantitatively / quantitatively detecting homocysteine. The strip is composed of an elongated upper support layer, an elongated lower support layer and test layers in the middle and is divided into a hand-held area and a test area. A diffusion layer, a filtration layer, an enzyme reagent layer and a colour development reagent layer are arranged in the test area from top to bottom. Various grid materials of synthetic fibre materials can be used to prepare the diffusion layer, various filterable materials for separating blood can be used to prepare the filtration layer, and various asymmetric synthetic membranes can be used to prepare the enzyme reagent layer and the colour development reagent layer. A loading hole is arranged in the upper support layer, a sample is sent from the loading pole to the diffusion layer, the sample then permeates into the filtration layer, the enzyme reagent layer and the colour development reagent layer, chemical reactions are performed in the enzyme reagent layer and the colour development reagent layer to change the colour, and the change of optical density in reaction process can be tested through a test pole of the lower support layer.

The present invention refers to articles for collecting samples from a surface, articles for microbiological analyses of said samples, and methods of use of said articles. The articles include sample collectors, sample housings with optional barrier layers, and sample-ready reagent strips comprising hydrophilic agents to grow and detect microorganisms. The disclosure includes methods to collect, detect, and quantify microorganisms in a surface sample.

The invention relates to the technical field of biology, and discloses an immunochromatography reagent strip for quantitative detection of 25-hydroxyvitamin D and a preparation method of the immunochromatography reagent strip. The immunochromatography reagent strip for quantitative detection of the 25-hydroxyvitamin D comprises a sample adding region, a conjugate release region, a reaction region and a water absorption region, which are sequentially arranged; the conjugate release region is a glass cellulose membrane enveloped with colloidal gold or a quantum dot or an up-conversion luminescent material UCP-marked mouse-anti-human 25-hydroxyvitamin D monoclonal antibody; the reaction region is a test region and a control region; the test region is enveloped with 25-hydroxyvitamin D-coupling protein; and the control region is enveloped with a nitrocellulose membrane of a goat anti-mouse IgG antibody. The immunochromatography reagent strip is capable of accurately, quantitatively, rapidly and sensitively detecting the 25-hydroxyvitamin D in human whole blood, serum and plasma; the required instrument is different from a traditional large expensive instrument for a clinical laboratory; and only one corresponding miniature immunity analyzer is required, therefore, real-time detection is realized.

An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement toward and away from a sample transfer position. Sample supplied to a sample well in the cassette body is taken up by a reagent reservoir containing a first reagent composition effective to form a modified sample. The support provides a reagent strip having a transfer zone that is brought into contact with the reservoir, when the support is in the transfer position, a detection zone located downstream of the transfer zone, and a second reagent composition effective to react with the modified sample to form a detectable analyte-dependent product. By controlling movement of the support, volume and rate of sample flow from the reservoir to the strip can be controlled to optimize and / or standardize sample-transfer conditions in the assay.

The invention relates to the field of biological detection and in particular provides a colloidal gold test strip for detecting canine parvovirus. The colloidal gold test strip comprises a canine parvovirus antigen-coated polyclonal antibody and two anti-IgG nitrocellulose membranes of two strips, and a glass fiber membrane containing colloidal gold-labeled canine parvovirus antigen. According to the colloidal gold test strip, the canine parvovirus antigen is detected by adopting a double-antibody sandwich method and immunchromatography assay, the colloidal gold test strip has the advantages that the specificity is high, the operation is simple, the detection is rapid and accurate, complex equipment is not needed, and the requirements on clinical examination can be met.

The invention relates to a magnetic bead reagent for a chemiluminescence immunoassay reagent. The magnetic bead reagent is prepared from immunomagnetic beads, a basic solution and a thickening agent.The physical form of the reagent is temperature-dependent, that is, the reagent is colloidal or solid under a certain temperature condition. The fluidity of the reagent is extremely low, the physicalform of the reagent is liquid as the temperature rises, the fluidity is restored, and the immunomagnetic beads are uniformly dispersed in the reagent and are not prone to settling. When the reagent isin a colloidal or solid form, the loss caused by adhesion of the immunomagnetic beads to packaging films or tube walls in the cold chain transportation process can be effectively avoided, and accordingly, detection accuracy is guaranteed; when the physical form is not liquid due to temperature change, the immunomagnetic beads are uniformly dispersed in the reagent and are not prone to settling. Consistency of the magnetic bead component reagent can be ensured during subpackaging. The magnetic bead reagent is suitable for luminescent immunoassay kits, especially kits or reagent strips for POCT(point-of-care testing).

A fully-automatic immunofluorescence quantitative analyzing apparatus and detection method belong to the field of quantitative immunofluorescence analysis and detection. The immunity quantitative analyzing apparatus includes a support baseplate, a reagent strip storage and automatic loading module, a cuvette ring module, a detection module, a sample module, a sample dispensing module, a washing module and a control system, the reagent strip storage and automatic loading module, the cuvette ring module, the detection module, the sample module, the sample dispensing module, the washing module being sequentially arranged on the support baseplate. The reagent strip storage and automatic loading module provides a reagent strip for the cuvette ring module, and the sample dispensing module dispenses the sample on the sample module to the cuvette ring module and performs a reaction, and the sample enters the detection module to complete the detection after the reaction is complete.

The invention provides a reagent strip for joint detection of a syphilis specific IgM and IgG antibodies and a preparation method thereof, relating to a reagent for detection of a syphilis specific antibody. The invention provides the reagent strip for joint detection of the syphilis specific IgM and IgG antibodies and the preparation method thereof. The reagent strip is provided with a vector plate, a loading pad, a colloidal gold pad, a nitrocellulose membrane, a syphilis specific IgG antibody detection line, a syphilis specific IgM antibody detection line, a contrast line and an absorption pad. The preparation method comprises the following steps: preparing sample application of nitrocellulose membranes for recombinant syphilis antigens TPN17 and TPN47, preparing colloidal gold, labeling syphilis specific antigen TPN17 and TPN47 with colloidal gold and preparing the reagent strip for joint detection of the syphilis specific IgM and IgG antibodies.

A hybridoma cell strain B9 (CGMCC No.0970), its monoclonal antibody resistant to human haematoglobin, the reagent strip containing said monoclonal antibody for the colloidal gold immunochromatographic test, and the preparing process of said reagent strip are disclosed. Its advantages are high sensitivity and high specificity.

A PCV2 antigen or antibody test paper consists of support layer which is water proof thin sheet layer and reaction reagent carrier absorption layer which comprises fibre layer, gold mark fibre layer and cellulose film layer in sequence from sample end as well as handle end being layer of water absorption material.