238 results about "Reagent strip" patented technology

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

The invention relates to the technical field of biological detection, in particular to an MxA, CRP and SAA combined detection card and a preparation method and application thereof, and the preparation method comprises the following steps: 1) preparing silicon-coated high-performance time-resolved fluorescent microspheres; (2) marking of an MxA antibody; (3) marking a CRP antibody; (4) marking an SAA antibody; (5) preparing a conjugate pad; (6) preparing a nitrocellulose membrane; and (7) assembling the reagent strip. The silicon-coated high-performance time-resolved fluorescent microspheres with the particle size range of 190-352 nm are prepared, the dye embedding rate is high, the sensitivity is high, the monodispersity is good, the stability is good, the hydrophilicity is high, agglomeration is not prone to occurring, and the dye embedding rate is increased. The MxA, CRP and SAA combined detection card prepared from the silicon-coated high-performance time-resolved fluorescent microspheres can improve the accuracy of identifying bacterial infection and virus infection, and is beneficial to early diagnosis and treatment of acute respiratory infectious diseases.

The utility model discloses an electrochemical luminescence lateral flow gene detection reagent strip and a kit, the electrochemical luminescence lateral flow gene detection reagent strip comprises an electrode and two hydrophilic channels A and B, and the electrode and the two hydrophilic channels are partially overlapped; the hydrophilic channels A and B are distributed on the sample adding sheet, the combination sheet, the detection sheet and the absorption sheet; the electrodes comprise an integrated bipolar electrode and a pair of driving electrodes; the integrated bipolar electrode comprises a cathode and at least two anodes which are connected in parallel; wherein the integrated bipolar electrode anode close to the sample adding sheet is called a quality control anode, and the integrated bipolar electrode anode close to the absorption sheet is called a detection anode. The electrode plate of the detection reagent strip is a closed bipolar electrode, and the anodes of the two bipolar electrodes are symmetrically distributed in parallel, so that the area of the electrode is reduced, the occupied space of the electrode is reduced, a plurality of detection anodes can be arranged, and the detection reagent strip has the potential of multi-path multi-element detection.

The disclosure discloses a liquid specimen lysis detection device. The device includes a detection unit, a lysis unit and a detachable limit structure, where the detection unit includes a detection shell, a sample acquisition unit and a detection reagent strip, the lysis unit includes a base, a piercing sleeve, a sealing film and a bottom bottle. The detachable limit structure is installed between the detection unit and the lysis unit to limit the depth of the detection unit inserted into the top opening of the base in the initial state, so as to prevent the piercing sleeve from piercing the sealing film. In the detection state, the detachable limit structure is removed, and the piercing sleeve pierces the sealing film under the push of the plug-in section of the detection shell; the sample acquisition unit extends into the bottom bottle.

The present invention discloses a pre-test processing device, medical equipment and a detection method, wherein the pre-test processing device includes a tray having at least one limiting groove; a tray fixing fixture having a structure for installing and fixing the tray; a material supply mechanism having a structure for storing materials in batches and transferring the materials one by one to the tray; a test liquid storage mechanism having a group of containers; and an injection mechanism having a structure that can extend into any of the containers to absorb liquid and inject it into the groove of the material on the tray. This solution installs reagent strips by setting a tray, and stores batches of materials through a material supply mechanism and transfers the stored materials to the tray to meet the test needs. Combined with the injection mechanism, the test liquid in the container is automatically injected into the groove of the reagent strip on the tray, which can meet the needs of large-scale and multi-item testing, create equipment conditions for the automation of various tests, and help improve detection efficiency.

The invention provides a light-excited lateral flow immunosensor reagent strip, a detection analysis method and application of the light-excited lateral flow immunosensor reagent strip. The reagent strip comprises an electrode substrate, a combination pad, a sample pad layer, a chromatography membrane and a water absorption pad, and the sample pad layer, the combination pad, the chromatography membrane and the water absorption pad of the reagent strip are connected end to end and are sequentially overlapped and pasted on the electrode substrate. The light-excited lateral flow immunosensor reagent strip is applied to quantitative analysis of various disease markers in biological fluids such as blood, saliva, cerebrospinal fluid or urine.

The present invention discloses a hs-TnI detection method based on quantum dot microspheres, which directly reacts a sample with a first solution containing antibody 1 and a second solution containing antibody 2 to generate a sandwich structure complex of antibody 1-antigen-antibody 2, and the antigen and antibody react in a reaction cup, incubate at a constant temperature, and the reaction time is longer, and the reaction is more sufficient, which is conducive to the improvement of detection sensitivity. At the same time, during chromatography, with the high affinity of streptavidin and biotin, the complex obtained by the reaction can be better captured on the T line, so that the reaction can be made more sensitive, and the detection time is also faster relative to the existing reagent strips. Further, combined with the advantages of high sensitivity and wide linear range of quantum dot microspheres, detection repeatability (CV) is better, CV is increased from the traditional 15wt% to within 8wt%, and the detection sensitivity is as high as 2pg / ml, while the linear range is 2-30000pg / ml, and full coverage is achieved for low-concentration and high-concentration samples.

The invention discloses a triiodothyronine dissociation agent, a detection kit, a preparation method of the triiodothyronine dissociation agent and application of the triiodothyronine dissociation agent and the detection kit, and the triiodothyronine dissociation agent comprises 10-100 mM of acetic acid-sodium acetate buffer solution, 0.05-1 g / L of ethylene diamine tetraacetic acid disodium salt, 1-10 g / L of perfluoroalkyl ethyl methacrylate, 0.2-1 mL / L of Tween-20 and 0.2-2 mL / L of ProClin 300, the pH (Potential of Hydrogen) of the triiodothyronine dissociation agent is 5.5 + / -1; the kit comprises a triiodothyronine dissociation agent and a reagent strip. According to the present invention, the two sites can be specifically recognized, the sensitivity and the specificity are high, the high-specificity detection on the specific antigen can be achieved, and the low-end sensitivity is high; meanwhile, the interference problem of rare earth europium during detection of triiodothyronine can be solved, and the accuracy of a detection result is further improved.

An optical system for a dry biochemical analyzer. The system consists of a light-source feedback unit, a light-homogenizing illumination light path and a collection light path. The electric current of a light source can be adjusted by means of the light-source feedback unit, such that the intensity of light output by the light source is stable. The system has a simple structure, high adjustment precision and high stability. By means of a multi-channel light-source current driver provided in the light-source feedback unit, rapid switching control over monochromatic light sources with different wavelengths can be realized. By means of the light-homogenizing illumination light path, light-homogenizing processing is performed on incident light beams, such that uniform illumination can be obtained on a dry reagent strip under test, the light path is concise, and a plurality of monochromatic light sources with different wavelengths that are provided off-axis can be overlapped and focused at the same position. The collection light path is configured as a telecentric light path, and a field diaphragm is provided, such that light beams on an entire illuminated surface can be uniformly collected. By using the system, the accuracy of collected reaction light beams corresponding to an object to be tested can be improved, thereby improving the accuracy of a subsequent analysis result of said object.

The utility model relates to the technical field of immunodetection, and provides a multi-channel detection reagent card which comprises a shell, the drainage channel comprises a plurality of sub-channels which are arranged in parallel; the drainage structure is provided with a plurality of drainage branches arranged in the circumferential direction of the sample adding opening, and each drainage branch is arranged to be matched with one sub-channel so as to drain samples at the sample adding opening into different sub-channels; the shunting structure is used for shunting the sample at the sample adding port to two sides and then enabling the sample to enter the drainage branches contained in different clusters; the detection reagent strips in different sub-channels can be used for detecting different target types such as bacteria, viruses and toxins at the same time. The multi-channel detection reagent card provided by the utility model can be used for detecting and screening various different virulent biological hazard factors in a sample at one time, and is beneficial to shortening the detection time and improving the detection efficiency; and the sample can be prevented from overflowing, and the sample is prevented from rushing into the sub-channel in a certain area to cause non-uniform distribution of the sample.

The invention provides multi-channel multi-joint inspection POCT full-automatic chemiluminescence equipment. The equipment comprises a rack; the sampling gun head accommodating mechanism, the test tube accommodating mechanism and the HGB detection mechanism are arranged on the rack in a longitudinal reciprocating motion manner, and a treating fluid container is arranged on the sampling gun head accommodating mechanism; the sampling mechanism is arranged on the rack in a transverse reciprocating motion manner; the reaction bin mechanism can be longitudinally arranged on the rack in a reciprocating manner and comprises a reaction bin body used for loading multiple joint inspection reagent strips, and the reaction bin body is provided with pressing elastic pieces used for pressing the multiple joint inspection reagent strips; the clamping and locking mechanism is located below the reaction bin mechanism, the reaction bin mechanism further comprises a driving device and a synchronous conveying belt connected with the driving device and a reaction bin body, and the clamping and locking mechanism is used for clamping, locking or loosening and unlocking the synchronous conveying belt so as to lock or unlock the reaction bin body. According to the present invention, the device has the immunoassay function, the HGB detection can be achieved, the multi-joint detection reagent strip does not shake or tilt, and the reaction bin body can be locked.

The invention relates to the field of reagent strip packaging quality detection, in particular to an in-vitro diagnostic reagent strip packaging quality control method and system. The method comprises the following steps: acquiring a packaged in-vitro diagnostic reagent monitoring image; performing multi-scale reconstruction on the in-vitro diagnostic reagent monitoring image so as to generate a multi-scale noise reduction reconstructed monitoring image; performing image visual segmentation and image iteration packaging defect detection on the multi-scale noise reduction reconstruction monitoring image one by one so as to obtain a packaging defect part expansion monitoring image; carrying out surface defect texture mining on the packaging defect part expansion monitoring image, and carrying out deep defect factor inference so as to generate packaging defect inference factors; obtaining a reagent strip packaging log; and defect process tracing is performed on the reagent strip packaging log according to the packaging defect inference factors, so that packaging process stages causing defects are marked. The packaging parameters are automatically adjusted through real-time feedback, so that the packaging quality of the in-vitro diagnostic reagent strip is accurately improved.

The invention discloses a serum amyloid protein A immunochromatography detection reagent strip which comprises a reaction bottom plate, a sample pad, a combination pad, a chromatography membrane and absorbent paper, the chromatography membrane is arranged in the middle of the reaction bottom plate, the sample pad and the absorbent paper are arranged at the two ends of the reaction bottom plate respectively, the chromatography membrane is arranged in the middle of the reaction bottom plate, the combination pad is arranged between the chromatography membrane and the sample pad, the sample pad is partially overlapped on the combination pad, and the combination pad is partially overlapped on one side of the chromatography membrane. The absorbent paper is partially overlapped on the other side of the chromatography membrane; quantum dot fluorescent microspheres used for serum amyloid protein A antibody labeling and quantum dot fluorescent microspheres used for DNP-BSA labeling are solid-phase on the combination pad; the chromatographic membrane is provided with a detection line coated with a serum amyloid protein A detection antibody and a quality control line coated with a rabbit anti-DNP quality control antibody; and a detection line and a quality control line are sequentially arranged on the chromatography membrane in parallel at intervals along the chromatography direction. The invention further discloses a corresponding preparation method, a detection kit, a detection method and application.

The invention discloses a food allergen visual detection chip preparation method, which comprises: using a nitrocellulose membrane as a carrier, coating an allergen component protein antibody dissolved in a PBS buffer solution system on the nitrocellulose membrane by using a chip spotting instrument, and drying to form a detection reagent strip; preparing a liquid reagent required by a reaction system, and subpackaging to form a liquid detection reagent; and assembling the detection reagent strip and the liquid detection reagent component into a complete kit. The invention has the following technical effects: high sensitivity and specificity: multiple allergens can be detected at the same time, and cross reaction is reduced; compared with a traditional method, the method has the advantages that the detection time is remarkably shortened, and the working efficiency is improved. Through a visual result, non-professionals can easily understand a detection result, and the attention of the public to food safety is promoted.

The present invention discloses a multi-index time-resolved fluorescence immunochromatography kidney injury detection kit, and relates to the technical field of kidney injury detection kits, the multi-index time-resolved fluorescence immunochromatography kidney injury detection kit comprises a box body, and the box body comprises a storage end, a detection end, a driving groove, a driving cap and a sealing cover; the detection unit comprises a plurality of groups of first reagent strips, second reagent strips, an erecting plate, a driving assembly and a liquid dropping table. The two groups of driving caps respectively drive the roller and the connecting rod to slide along a specified path, so that a detector can flexibly adjust the position of a reagent strip. Kidney sample liquid is sequentially dripped on the dripping holes by utilizing the adsorption tube for chromatographic detection, and the injury range is accurately judged by comparing indexes on the liquid dripping table. If further checking is needed, the reagent strip can be easily replaced through the extraction groove, repeated detection is realized, and the result reliability is ensured. According to the design, the detection efficiency and accuracy are improved, and the operation convenience and flexibility are enhanced.

The embodiment of the utility model provides a biological detection reagent strip storing and pushing device, which relates to the technical field of reagent strip storing and pushing devices, and comprises a reagent strip pushing device and a rotating bin inwards arranged on the front side of the reagent strip pushing device, reagent grooves are evenly distributed in the surface of the rotating wheel, a kit can be placed in the rotating wheel through the reagent grooves, a discharging frame is fixed to one side of the bottom face in the rotating bin, a discharging pipe used for discharging is fixed to the top of the discharging frame, and meanwhile a push rod capable of pushing the kit to move is further arranged on one side of the outer surface of the rotating wheel. And along with rotation of the threaded rod, one end of the push rod pushes the kit to move into the discharging pipe, and then the kit can be conveniently taken out.

The utility model relates to the technical field of biological detection, in particular to a fluorescent PCR (Polymerase Chain Reaction) nucleic acid detection reagent strip for detecting Listeria monocytogenes, which is characterized in that after a sample to be detected is added into the reagent strip through a sample adding hole, the sample flows into a nucleic acid extraction area for nucleic acid extraction, and the extracted nucleic acid enters an amplification reaction area for amplification reaction; when a target nucleic acid sequence is amplified and then enters fluorescence detection to generate a fluorescence signal, a sensor in a fluorescence detection area captures the fluorescence signal in real time and transmits the signal to a control chip, the control chip processes and analyzes data, and a result is transmitted to external equipment (such as a computer, a mobile phone and the like) through a data transmission module. The rapid detection on the listeria monocytogenes is realized. The reagent strip disclosed by the utility model integrates the functions of nucleic acid extraction, amplification reaction and detection, is simple and convenient to operate, does not need complex sample pretreatment and professional experimental equipment, and is suitable for on-site rapid detection and basic application.

The utility model discloses a detector test strip fixing module convenient for non-professionals to use, which relates to the technical field of fast detection detectors and comprises a base and an insertion piece. When the kit is used, the bottom end of a reagent strip is fixed in the fixing groove and is matched with the fixing block to fix the reagent strip, and during detection, the sampling rod punctures the thin sheet A between the solution cavity and the communication cavity from top to bottom, so that a diluent with a sample enters the communication cavity and reaches the other side of the insertion sheet through the through hole; due to the fact that the bottom of the reagent strip is fixed in the fixing groove and makes contact with the diluent passing through the through hole, detection is achieved, the diluent is blocked below the first water blocking baffle and does not make direct contact with other parts of the reagent strip, and the purpose that the diluent gradually seeps to the top of the reagent strip is achieved; and therefore, the operation convenience and the accuracy of a detection result during detection by a non-professional user are further improved.

The utility model relates to the technical field of CTC detection, and discloses a CTC sample loading chip from above, including a cell filter and a reagent strip, and also including a sample loading gun head and a gun head seat, wherein the two sides of the top of the reagent strip are respectively provided with a first placement hole and a second placement hole, the gun head seat is arranged in the first placement hole, and the sample loading gun head is adapted to the gun head seat, the cell filter is adapted to the second placement hole, and a plurality of reagent placement cavities opened on the reagent strip are arranged between the first placement hole and the second placement hole. The utility model changes the liquid adding method in the prior art from aspiration to sample loading from above, which can not only ensure the stability and accuracy of the amount of liquid added, but also has a faster speed than sucking liquid from below, thereby reducing the detection time.

The present invention provides a carrier, which includes a channel for accommodating a test strip. One end of the channel is closed and the other end has an opening, and the fluid sample to be detected can enter the channel through the opening. Wherein, a flood prevention structure is included in the channel. By changing the channel in the carrier structure, the flood phenomenon of the test strip can be prevented and the error rate can be reduced.

The utility model relates to the technical field of medical instruments, in particular to a urine bag urine indicator which comprises a urine bag body, a detection tube assembly is arranged on one side of the bottom of the urine bag body, the detection tube assembly comprises a tube body, an insertion groove is formed in one side of the tube body, the urine bag body is communicated with the tube body, a detector is arranged on one side of the urine bag body, and the insertion groove is communicated with the insertion groove. A reagent strip is inserted into the detector, and a detection area of the reagent strip extends into the tube body through the slot. When the urine bag is used, the detection tube assembly is arranged on one side of the bottom of the urine bag main body, the urine bag is communicated with the detection tube assembly, and urine is transferred into the detection tube assembly from the urine bag, so that the following reagent strip detection work is facilitated, the detector is arranged on one side of the urine bag main body, and the insertion groove is formed in one side of the tube body; after the reagent strip is inserted into the detector, the detection area of the reagent strip extends into the tube body through the slot, so that the risk that urine is polluted during detection is reduced.

The utility model provides a colloidal gold reagent strip for detecting aminoglycoside antibiotics, which comprises a shell and a colloidal gold reagent strip, and the upper opening of the shell is connected with an upper cover in a sliding manner; a detection chamber is formed in the shell, the interior of the detection chamber is rotatably connected with a driving shaft and a driven shaft, and one end part of the driving shaft penetrates through the inner wall of the detection chamber, extends to the exterior of the shell and forms a screwing part; the surface of the driving shaft is sleeved with one end of a transmission belt, the surface of the driven shaft is sleeved with the other end of the transmission belt, a plurality of colloidal gold reagent strips are pasted on the surface of the transmission belt, and sample adding grooves, observation grooves and type display grooves are formed in the positions, corresponding to the colloidal gold reagent strips, of the top of the upper cover; according to the utility model, the types of required colloidal gold reagent strips can be selected according to different samples, the required reagent strips can be selected for multiple times and the samples can be continuously dropped through the sample adding groove when multiple experiments are carried out, the results of a plurality of reagent strips can appear at the same time, and the experiment efficiency is improved.

The invention relates to the technical field of europium-labeled antibody preparation, in particular to a europium-labeled antibody dilution solution reagent strip preparation device and a preparation method thereof.The europium-labeled antibody dilution solution reagent strip preparation device comprises a supporting frame, a mixing tank is installed on one side of the top of the supporting frame, a reagent strip is arranged in the supporting frame, a discharging pipe is fixed to the bottom of the mixing tank, and a smearing assembly is arranged at the bottom of the discharging pipe; the smearing assembly comprises a material guiding frame fixed to the bottom of the discharging pipe, sliding grooves are formed in the two ends of the material guiding frame, sliding blocks are arranged on the inner walls of the sliding grooves in a sliding mode, sealing plates attached to the inner walls of the two ends of the material guiding frame are fixed to one ends of the two sliding blocks, and smearing roller pieces are rotationally connected to the bottoms of one sides of the two sealing plates; the smearing roller piece has the stirring performance in the smearing process, a certain extrusion effect is formed on a reagent strip and a liquid absorption layer, the smearing roller piece better adapts to the surface of the reagent strip, smearing uniformity is guaranteed, and the preparation quality of the reagent strip is improved.

The invention relates to the technical field of nucleic acid extraction instruments, in particular to a full-automatic nucleic acid extraction instrument, a portal frame module comprises a horizontally arranged portal frame base and a portal frame vertically arranged on the portal frame base, and the portal frame is slidably connected to the portal frame base; an oscillation module, a reagent strip bracket for placing a reagent strip and a reaction tube bracket for placing an eight-row tube are arranged at the upper part of the gantry base; the oscillation module is arranged on the oscillation module gantry base, and cell disruption of the sample is completed by the oscillation module through oscillation movement; one side of the gantry bracket is connected with the extraction module in a vertical sliding manner, and the other side is connected with the pipetting module in a vertical sliding manner; the pipetting module is used for transferring the sample subjected to cell wall breaking into each hole site in the reagent strip; the extraction module is used for extracting nucleic acid from the sample; and the pipetting module is also used for transferring the nucleic acid sample into the eight-row tube. The automation of the whole nucleic acid extraction process is realized.

The utility model provides a full-automatic plasmid purification instrument which comprises a base, an oscillation device, a reagent strip device, a first moving device and a liquid injection device are arranged on the base, a washing area and an elution area are arranged on the reagent strip device, and the liquid injection device is used for injecting reagents into hole sites in the oscillation device; the first moving device is provided with a magnetic bar magnetic sleeve part, the magnetic bar magnetic sleeve part comprises a moving frame connected with the first moving device, the moving frame is provided with a liftable magnetic sleeve and a magnetic bar, and the magnetic bar can extend into the magnetic sleeve; the movable frame is further provided with a cutting device. When the escherichia coli plasmid DNA extraction and purification device is used for extracting and purifying escherichia coli plasmid DNA, the steps of liquid injection, oscillation, magnetic bead extraction, cleaning, elution and the like can be automatically completed, manual intervention is not needed in the whole extraction and purification process, the labor cost is saved, and the working efficiency is high; and the whole extraction and purification process does not need manual operation intervention, so that the risk that the reagent is polluted due to improper manual operation is reduced.

The application discloses a kind of assembly tooling applied to immune chromatography reagent strip large plate, it is related to biomedical engineering industry technical field, including bottom plate and electric rotary table, the bottom plate top is fixedly connected with numerical control mechanical arm, the electric rotary table top is provided with disc, the disc top is provided with large plate clamping mechanism, by setting large plate clamping mechanism, the sliding slide of loading PVC bottom plate and clamping block will go along with the centrifugal force that rotates and is outward synchronous disc outer ring sliding, sliding slide slides on sliding strip, so that the clamping block on sliding slide and PVC bottom plate are outwardly slid along with PVC bottom plate, the extrusion force between the pad on PVC bottom plate and clamping block is relieved when being subjected to outward centrifugal force, avoid the problem that the pad on PVC bottom plate slides on clamping block and is warped, and each numerical control mechanical arm is placed on each kind of pad on sliding slide and is vibrated flat, prevent deviation and cause pad to be warped and skew problem when being placed.

The invention discloses a composite biological blocker for immunochromatographic detection, a preparation method thereof and a reagent strip, wherein the blocker includes a blocker buffer, wherein mouse monoclonal antibody IgG-1, mouse monoclonal antibody IgG-2, goat anti-human IgM / RF polyclonal antibody and rabbit polyantibody IgG are diluted in the blocker buffer; BSA is also added to the blocker buffer; wherein the blocker buffer is tris-HCl buffer or phosphate PBS buffer. The blocker can effectively reduce or eliminate the adverse effects of endogenous interfering substances on fluorescent immunochromatographic detection, and prevent the occurrence of false positive and false negative test results; after mixing several different mouse monoclonal antibodies, goat polyantibodies and rabbit polyantibodies, they are used for immunochromatographic detection, which can effectively prevent and control the influence of endogenous interfering substances in the sample on immunochromatographic detection, and improve the accuracy of the detection results of immunochromatographic quantitative detection reagents.

The invention provides a progesterone detection reagent strip based on an antibody synergistic effect and a preparation method of the progesterone detection reagent strip. The progesterone detection reagent strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the combination pad is sprayed with fluorescent nano-microspheres coupled with a goat-derived PROG antibody and fluorescent nano-microspheres coupled with a goat anti-rabbit antibody, a detection line of the nitrocellulose membrane is coated with a PROG antigen, and a quality control line of the nitrocellulose membrane is coated with a rabbit anti-goat IgG antibody. According to the progesterone detection reagent strip based on the antibody synergistic effect, the competition method detection principle is adopted, the fluorescent microspheres are used as markers, the progesterone antibody and the fluorescent microspheres are combined in a coupling mode, the specificity and binding efficiency of antigen-antibody reaction are optimized based on the antibody synergistic effect, and the progesterone detection reagent strip can be used for detecting progesterone. The stability and sensitivity of the detection system are remarkably improved, and high-sensitivity and high-specificity detection of the progesterone level in serum or plasma is achieved.

The present invention relates to the technical field of allergen detection, and discloses a high-sensitivity detection reagent strip, a preparation method, and its application, comprising a shell, the shell comprising at least a sample addition hole, a liquid addition hole, and a display area; a lateral flow test strip is provided inside the shell; the lateral flow test strip comprises a bottom structure, the bottom structure is provided with a marker flow area and a sample flow area; wherein the marker flow area is located above or below the sample flow area; the sample flow area and the marker flow area each comprise at least one sample pad, and a plurality of the sample flow areas and the marker flow areas are physically isolated from each other. The beneficial effects of the present invention are: it can realize combined immunoassay and quantitative detection, solves the problem that the traditional mode cannot be quantified or is too time-consuming, and further increases the sensitivity of the product due to the increase in the sample incubation process.

The invention relates to the technical field of medicine and biology, and discloses a xerophthalmia multi-item joint detection kit which is characterized in that the kit is composed of a detection plate, sample treatment liquid, disposable tear detection filter paper strips and a drying agent, the detection plate comprises at least one detection reagent strip, and the sample treatment liquid is arranged in the detection reagent strip. The detection reagent strip is composed of a nitrocellulose membrane, a sample pad, a reagent pad, absorbent paper and a PVC (polyvinyl chloride) board, the reagent pad is a marking solution prepared by respectively marking an MMP-9 antibody, an IgE antibody and an LF antibody by a nanocarbon method, the marking solution is spread on glass fibers after being diluted by a diluent, and the glass fibers are dried overnight at 37 DEG C. According to the invention, the problems of instability and low sensitivity of colloidal gold detection products in the current market are solved. The kit is simple to operate, high in specificity during detection, high in sensitivity and visual in result, and is suitable for home self-test.