1763 results about "Trehalose" patented technology

Disclosed is a pharmaceutically-acceptable composition in the form of a solid, amorphous, mono-particulate powder comprising a mixture of:a pharmacologically-effective dosage amount of an adrenergic receptor modulator, or a pharmaceutically-acceptable salt thereof; anda pharmaceutically-acceptable carrier material, which carrier material comprises a maltodextrin with a dextrose equivalent (DE) that is above 15.Compositions are suitable for transmucosal drug delivery, including nasal delivery, by which said compositions may be loaded into a single-use nasal applicator. Compositions are preferably made by way of spray drying and may further include a disaccharide, such as lactose or trehalose which, along with the active ingredient and maltodextrin, may be spray-dried together in combination. Compositions may further comprise one or more alkyl saccharides. Preferred alkyl saccharides include sucrose esters, such as sucrose monolaurate. Preferred adrenergic receptor modulators include epinephrine (adrenaline). The compositions are thus particularly useful in the treatment of allergic reactions, including anaphylaxis.

According to the invention, there is provided a which composition is in the form of an amorphous, mono-particulate powder comprising a mixture of: (a) a pharmacologically-effective dosage amount of at least one biopharmaceutical drug compound; and (b) a pharmaceutically-acceptable carrier material, which carrier material comprises a combination of a disaccharide and a polymeric material. Preferred pharmaceutically-acceptable carriers in this regard include lactose or trehalose and dextrins (e.g. maltodextrins). Compositions may further comprise one or more alkyl saccharides. Preferred alkyl saccharides include sucrose esters, such as sucrose monolaurate. Powder compositions may be produced by spray-drying the various components together in combination.

The invention relates to the technical field of freeze-dried preparations, and provides exosome freeze-dried powder as well as a preparation method and application thereof. The exosome freeze-drying preparation is prepared by mixing an exosome solution and a freeze-drying protection solution and then freeze-drying the mixture, the concentration of the exosome solution in the mixed solution is 0.1-10% V / V, and the freeze-drying protection solution comprises 0.4-4% W / V of saccharides and 0.25-4% W / V of alcohols; the saccharide is trehalose, cane sugar or lactose; the alcohol is mannitol, sorbitol or a combination of mannitol and polyethylene glycol; and the solvent is sterilized water for injection or a PBS buffer solution. The exosome freeze-dried powder is stable in activity, small in irritation, good in safety and good in fibroblast proliferation promoting capacity, when the exosome freeze-dried powder is applied to supporting essence or masks in the medical beauty industry, the exosome activity of the exosome freeze-dried powder is still kept stable, and the application prospect is good.

The invention belongs to the technical field of biology, and discloses a stable protective agent, a cell antigen dry sheet and a preparation method and application of the dry sheet, the stable protective agent comprises glycerin with the volume fraction being 0.4%, gelatin with the mass volume fraction being 0.1%, EDTA with the mass volume fraction being 0.1 mM, trehalose with the mass volume fraction being 0.03% and resveratrol with the mass volume fraction being 100 [mu] M. The stable protective agent is adopted for preparing the cell antigen dry sheet, and the cell antigen dry sheet is applied to CBA detection. The stable protective agent has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent cell deformation and cell fixing layer shedding, and can well maintain the stability and reliability of antigen proteins, and the prepared cell antigen dry sheet can be stored for a long time, and has a good application prospect. The antigenicity of a membrane signal index is not damaged. When a positive sample is detected, obvious green fluorescence cells can be seen, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed conveniently.

The invention provides a method for biologically removing toxicity inhibition and intensively degrading typical medical organic wastewater under salt stress, and belongs to the technical field of sewage treatment. According to the method, efficient degradation of the high-salt medical organic wastewater is achieved by taking measures such as combination of salt-tolerant nascent-state functional flora and cofactor strengthening, the salt-tolerant nascent-state functional flora comprises obligate COD degradation anaerobic granular sludge and sludge domesticated by gradient salinity pressure of medical wastewater, and the cofactor is betaine, glutamic acid, trehalose or potassium chloride. According to the method, the anaerobic methanogenesis efficiency and TOC removal of organic matters in a degradation system under salt stress can be promoted, the inhibition effect of salt on flora can be relieved and broken through the addition of cofactors, effective metabolism of pollutant nitrogen heterocyclic rings and alkane substances is promoted, refractory characteristic components of wastewater are eliminated, and the biodegradability of the wastewater is improved.

The invention provides a composition with a blood sugar reducing function and application thereof, and belongs to the technical field of diabetes treatment. The composition is prepared from burdock root extract, folium ilicis purpurea extract, hypoglycemic decapeptide, trehalose, sodium benzoate, glycerol, vitamin C and water. The composition disclosed by the invention has a multi-target synergistic effect, and by integrating natural plant extracts and functional peptides, multiple targets related to diabetes mellitus are adjusted, so that the blood sugar level is remarkably reduced, and cholesterol metabolism, oxidative damage and inflammatory response are effectively improved. The composition can be used for treating type 2 diabetes mellitus and has a good clinical application prospect.

The invention relates to the field of skin care and medical cosmetology, and discloses a long-acting moisturizing and barrier repairing type exosome active gel and a preparation method thereof, and the gel comprises the following components by mass: 2-5 parts of exosome; 3 to 7 parts of trehalose; 2 to 6 parts of glycerol; 1 to 2 parts of polyvinyl acrylate; 0.5 to 1.5 parts of octyl glucoside; 0.8 to 1.5 parts of sodium hyaluronate; 0.2 to 0.5 part of low molecular peptide; 1 to 3 parts of polyacrylamide; and 0.02 to 0.05 part of a tea polyphenol antioxidant. According to the invention, through synergistic repair of the exosome and the low-molecular peptide, dual moisturizing of the trehalose and the glycerol, a thickening system is innovated to improve the stability, and the tea polyphenol antioxidant is combined, so that deep repair, lasting moisturizing, skin feeling optimization and aging resistance are effectively realized, and multiple bottleneck problems of a traditional skin care technology are comprehensively solved.

The invention relates to a concrete anti-cracking self-repairing agent in the technical field of building materials, and aims to solve the problem that concrete cracks are difficult to self-repair. According to the technical scheme, the self-repairing agent is characterized by comprising a microbial repairing unit formed by ZSM-5 type zeolite molecular sieve loaded bacillus pasteurii spores, mesoporous silica particles loaded with L-calcium aspartate and Fe3O4 (at) SiO2 core-shell nanoparticles which are fixed through an in-situ polymerization coating layer to form a magnetic thermal response capsule layer coating the surface of the microbial repairing unit, pLGA microspheres containing yeast extract are positioned on an interface of zeolite and hydrogel, and a trehalose-anhydrous calcium chloride eutectic compound is used as a directional mineralization accelerant. The concrete anti-cracking self-repairing agent provided by the invention is mainly used for intelligent identification and self-repairing of concrete cracks, and efficient repairing is realized through a microbial mineralization, calcium ion slow release and magnetic thermal response synergistic mechanism.

The invention relates to a functional microorganism-containing organic-inorganic compound fertilizer and a production method thereof, and relates to the technical field of fertilizers, the functional microorganism-containing organic-inorganic compound fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer, the functional microorganism outer layer comprises the following components in parts by mass: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of a yeast extract and 30-40 parts of a modified activated carbon carrier. The production method comprises the following steps: preparing an inorganic core layer, preheating the inorganic core layer, and spraying and coating an organic coating layer material to obtain a primary product with an organic coating layer; and coating the surface of the organic coating layer of the primary product with a functional microorganism outer layer material, drying at low temperature, and screening to obtain the organic-inorganic compound fertilizer containing the functional microorganisms. The fertilizer has the effect of improving the slow release effect of the fertilizer and the survival rate of microorganisms.

The invention discloses a magnetic biochar-based saline-alkali soil conditioner as well as a preparation method and application thereof, after a biochar material is loaded on ferrous iron, the biochar material is subjected to alkaline oxidation to obtain ferroferric oxide and a porous biochar material in a stable state, and finally activated humic acid, betaine, trehalose and melatonin are combined with magnetic biochar. The magnetic charcoal-based saline-alkali soil conditioner is low in price and easy to obtain and has a good improvement effect on physicochemical properties of saline-alkali soil and promotion of rice growth, and the preparation process is simple and easy to operate.

The invention relates to the field of nutrient solutions, and particularly discloses a high-calcium-zinc dietary nutrient solution for promoting skeletal development and a preparation method thereof, the high-calcium-zinc dietary nutrient solution for promoting skeletal development comprises the following raw materials: calcium citrate, zinc citrate, a zinc-containing yeast extract, vitamin D3, a nutrition enhancer, functional oligosaccharide, xanthan gum, anhydrous citric acid, essence, a sweetening agent and water; the preparation method comprises the following steps: mixing the zinc citrate, the zinc-containing yeast extract, the seaweed meal, the natto powder, the stachyose and the trehalose to prepare a mixture A; uniformly premixing stevioside, xanthan gum and trehalose to obtain a mixture B; s4, vitamin D3 powder and trehalose are premixed and dissolved, and a mixture C is prepared; heating the residual water, adding the mixture A, the fructo-oligosaccharide, the anhydrous citric acid, the calcium citrate, the mixture B, the trehalose and the mixture C, adding the essence, and stirring to obtain the beverage. The preparation method has the characteristic of preparing the liquid calcium-zinc dietary nutrient solution with good absorption and utilization rate and palatability.

The invention relates to preparation of high-stability mascarpone cheese and application of the mascarpone cheese in baking, and belongs to the technical field of cheese preparation. The glutamine transaminase is combined with a citric acid-sodium citrate system, so that the excessive denaturation of protein is reduced, and the texture and flavor of the cheese are improved. Trehalose and xanthan gum are added for locking water and resisting freezing, and an antioxidant is combined for use, so that the freeze-thaw stability and oxidation resistance of the cheese are improved, the cheese is protected from texture change and oxidative damage in the freezing and unfreezing processes, and excellent texture and flavor can still be kept after multiple times of circulation. Arabic gum is modified through octenyl succinic anhydride, amphipathy is obtained, whey separation is prevented, whey loss is reduced, and the cheese taste is improved.

The application relates to an organic-inorganic compound fertilizer containing functional microorganisms and a production method thereof, and relates to the technical field of fertilizers. The fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer. The functional microorganism outer layer comprises the following components in mass fractions: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of yeast extract and 30-40 parts of modified active carbon carriers. The production method comprises the following steps: preparing the inorganic core layer, preheating the inorganic core layer, spraying the organic coating layer material to coat the surface, and obtaining a preliminary product with the organic coating layer; coating the functional microorganism outer layer material on the surface of the organic coating layer of the preliminary product, low-temperature drying and screening, and obtaining the organic-inorganic compound fertilizer containing the functional microorganisms. The application has the effects of improving the slow-release effect of the fertilizer and the survival rate of the microorganisms.

The invention discloses a blood genome DNA extraction kit and an extraction method, and belongs to the technical field of nucleic acid extraction. The DNA extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution and an eluent, wherein the lysate is prepared from the following components: 5.5 to 6 M of guanidine hydrochloride, 0.2 to 0.3 M of NaCl, 0.01 to 0.015 M of EDTA (Ethylene Diamine Tetraacetic Acid), 0.01 to 0.015 M of glucan, Triton X-100 with the volume concentration of 1.5 to 2 percent, 0.06 to 0.1 M of dithiothreitol, Tween-20 with the volume concentration of 5 to 10 percent and 10 to 50 mM of trehalose. The components of the lysate in the kit are optimized, through the synergistic effect of the components, the cell lysis efficiency is remarkably improved, meanwhile, DNA molecules higher in purity, more complete in structure and higher in extraction yield can be obtained, and therefore the kit has good application prospects.

The invention relates to a detection kit for an autoimmune encephalitis antibody and a preparation method of the detection kit. The detection kit for the antibody against myasthenia gravis comprises the following components: a myasthenia gravis antibody detection plate, a working solution, PBS (Phosphate Buffer Solution), a fluorescent secondary antibody and a mounting medium. According to the technical scheme, the carboxymethyl cellulose modified trehalose is used as the carrier of the antibody detection material to adsorb and wrap the cells, so that the fixing efficiency and stability of the cells on the detection carrier are remarkably improved, and the biological activity maintaining capability of the cells is enhanced, thereby improving the sensitivity and specificity of antibody detection and further improving the detection accuracy of the antibody. The carboxymethyl cellulose modified trehalose also can provide good biocompatibility, has excellent surface stability, can maintain the natural conformation and function of cells in the detection process, and effectively reduces the detection error caused by antigen denaturation or degradation.

The invention provides a cyclodextrin glucosyltransferase mutant and application thereof in preparation of trehalose, belongs to the technical field of gene engineering and enzyme engineering, and mainly provides the cyclodextrin glucosyltransferase mutant, amino acid mutation sites are 33rd and 21st amino acids of a cyclodextrin glucosyltransferase amino acid sequence SEQ ID NO.2; the disproportionation characteristic enzyme activity of the cyclodextrin glucosyltransferase mutant is obviously improved compared with that of a wild type, the thermal stability of the cyclodextrin glucosyltransferase mutant has obvious advantages, the optimum temperature of the mutant is 60 DEG C and is improved by 5 DEG C compared with that of the wild type, the cyclodextrin glucosyltransferase mutant is applied to preparation of trehalose through compounding of a multi-enzyme system, the yield of trehalose is obviously increased, and the application prospect is wide. The utilization rate of a substrate is increased, and meanwhile, the application prospect of the enzyme in medicine, food and biological industries is also improved.

The invention relates to a natural antioxidant for prefabricated vegetables and a preparation method of the natural antioxidant, and belongs to the technical field of food additives. The antioxidant is prepared from rosmarinic acid, carnosic acid, drug-loaded cellulose and double-phase chelating microspheres; the drug-loaded cellulose is prepared by modifying cellulose with quercetin, loading anthocyanin and then wrapping with a trehalose-gamma-polyglutamic acid composite film; the biphase chelating microspheres are phytic acid-citric acid biphase sustained-release microspheres formed by wrapping phytic acid and citric acid with a chitosan and sodium alginate composite film. Through multi-component functional complementation, multi-stage slow release design and physical-chemical synergistic barrier, comprehensive inhibition of the oxidation problem of the prefabricated vegetable is realized, a'free radical scavenging-metal chelating-phase interface stabilization 'triple protection system is constructed, accurate oxidation resistance in different stages of processing, storage and transportation is realized, and the prepared vegetable has good market prospects. The bottlenecks of short action period and single protection dimension of a natural antioxidant are broken through.

The invention relates to the technical field of cell culture media, in particular to a marine invertebrate cell culture medium. Comprising the following components in parts by weight: 0.5 to 1 part of a compound supplement, 1 to 4 parts of glucose, 0.3 to 0.5 part of L-glutamine, 0.01 to 0.05 part of vitamin, 10 to 15 parts of fetal calf serum, 0.02 to 0.04 part of penicillin, 0.01 to 0.03 part of trehalose, 0.3 to 0.7 part of a sodium chloride osmotic pressure regulator, 0.5 to 1 part of inorganic salt and 10 to 25 parts of a sodium bicarbonate buffer agent, wherein the compound supplement is prepared by mixing meson, diatom and disodium cyclohexane diamine tetraacetate according to the mass ratio of 1: (0.2-0.6): (0.1-0.5), the application range of the culture medium is widened by adopting the compound supplement, the meson and the diatom are added into the cell culture medium after enzymolysis, and compared with a chemically synthesized culture medium, the culture medium better meets the requirements of marine invertebrate cells; therefore, cell growth is effectively promoted, and the purposes of rapid growth and stable passage are achieved.

The invention discloses a composition containing PDRN microspheres as well as a preparation method and application thereof, and relates to the technical field of repair. The composition containing the PDRN microspheres is prepared from the following components in parts by weight: 1 to 10 parts of the PDRN microspheres, 30 to 60 parts of sodium hyaluronate, 0.05 to 1 part of a cross-linking agent, 0.5 to 5 parts of bisabolol and 1 to 8 parts of trehalose, the molecular weight of the sodium hyaluronate is 300 kDa to 800 kDa. By adding the PDRN microspheres and selecting reasonable component compatibility, the stability is remarkably improved, and a synergistic effect is reflected in the aspect of repairing.

The invention belongs to the technical field of stem cell biological preparations, and particularly relates to an anti-aging compound preparation based on stem cells and application of the anti-aging compound preparation in delaying skin aging. The composition is composed of an inner water phase, a middle oil phase and an outer water phase, wherein the inner water phase is prepared from the following components: stem cell exosomes, hyaluronic acid, nicotinamide ribose, trehalose, mannitol, glycine and a citric acid buffer solution; the medium oil phase is prepared from the following components: medium chain triglyceride, shea butter, vitamin E succinate, PEG (Polyethylene Glycol) modified ceramide NP and cholesterol succinic acid monoester; the external water phase is prepared from the following components: polydatin, cellulose nanocrystals and an emulsifying agent. On the basis of a W / O / W emulsion system, a multi-layer anti-aging mechanism is constructed by utilizing a three-dimensional delivery framework of an inner water phase, an oil phase and an outer water phase and space-time synergy of active ingredients, the exosome permeation efficiency is improved through microneedle introduction, and the anti-oxidation and barrier repair effects are achieved by using multiple ingredients.

The invention discloses a diluent for whole blood lateral immunochromatography detection as well as a preparation method and a detection method thereof, and belongs to the technical field of immunochromatography detection. The diluent solves the technical problems of unsmooth chromatography, background interference, poor stability of detection results and the like caused by red blood cells during direct detection of whole blood samples. The diluent comprises a buffer system, an osmotic pressure regulator glycine, a nonionic surfactant polysorbate, an erythrocyte aggregation inducer polyvinylpyrrolidone, a dynamic viscosity regulator trehalose, a blocking protein, a stabilizer combination L-methionine and L-histidine, and a composite formula of water. Red blood cells are effectively induced to gather and intercept on the sample pad through the synergistic effect of all the components, and meanwhile the stability of the chromatography process and the activity of the detection reagent are maintained. The diluent is mainly used for lateral immunochromatography quantitative detection of markers such as pro-enkephalin, C-reactive protein, cardiac troponin I or N-terminal pro-brain natriuretic peptide in a whole blood sample.

The invention relates to the technical field of health-care products, in particular to a weight-losing and lipid-lowering health-care product and a preparation method thereof. The weight-losing and lipid-lowering health-care product comprises chitosan, L-carnitine tartrate, polypeptide, a sweetening agent, an auxiliary protective agent and a filling agent. The invention also provides a preparation method of the composition. Compared with the prior art, the method has the advantages that the white kidney bean enzymatic hydrolysate, the trehalose and the plant extract are used as fermentation substrates for mixed fermentation, and the plant extract contains a large number of active ingredients which have the effect of promoting proliferation of probiotic strains and promoting fermentation, so that a large number of nutrient substances in the white kidney beans are dissolved out; further degrading into nutrient substances such as polypeptide, glucose, amino acid and the like which are easily absorbed by human beings; meanwhile, a large number of beneficial nutrition molecules are generated during mixed fermentation of the plant extract and the white kidney bean enzymatic hydrolysate, a large number of lipid-lowering substances can be generated, and meanwhile the good weight-losing effect is achieved.

The invention discloses bacillus pumilus, a complex microbial inoculant and application of the complex microbial inoculant in a saline-alkali soil four-dimensional improvement method, and belongs to the technical field of microorganism application. According to the invention, a strain of bacillus pumilus is screened, and the bacillus pumilus has excellent saline-alkaline tolerance, has multiple functions of producing organic acid, proline, siderophore, trehalose, IAA and betaine and the like, and has obvious advantages in saline-alkali soil improvement; the stress resistance of plants is improved through multiple mechanisms, nutrient absorption is promoted, the soil structure and chemical environment are improved, the stability and diversity of soil microbial communities are enhanced, and then effective treatment on the saline-alkali soil is achieved. According to the four-dimensional improvement method for the saline-alkali soil, the microbial technology, the physical technology, the chemical technology and the nutrition technology are combined, the saline-alkali soil is improved from the four dimensions of physics, chemistry, biology and nutrition, soil can be effectively improved, and repeated planting is not likely to happen.

The invention discloses a recombinant III-type human collagen composition. The recombinant III-type human collagen composition comprises recombinant III-type human collagen, a first buffer solution and a stabilizer, the stabilizer is selected from at least one of glycerol, butanediol, pentanediol, mannitol and trehalose. According to the recombinant III type human collagen composition, a chemical cross-linking agent or an enzymatic promoter is not added for cross-linking, and the used reagents are free of cytotoxicity, sensitization and other safety risks. Therefore, the recombinant III-type human collagen composition has no crosslinking agent residue, can form a three-dimensional network structure, can avoid the problem that the recombinant III-type human collagen and the polysaccharide are compounded to generate precipitates, also has good biocompatibility, degradability and high stability, especially has relatively high thermal stability, and can be used for preparing the recombinant III-type human collagen composition. The wound healing and repairing can be promoted, the healthy skin ecology can be reconstructed, and a new choice is provided for a biomedical material.

According to the high-temperature-resistant nitrogen-fixing bacteria microcapsule fertilizer and the preparation method thereof, aiming at the problems of thallus inactivation and nutrient loss in a high-temperature environment, a sodium alginate-chitosan double-layer embedding technology is combined with magnetic nanoparticle modification, and a stable double-layer structure is formed by optimizing the ratio of an embedding material, so that the high-temperature-resistant nitrogen-fixing bacteria microcapsule fertilizer is prepared. The survival rate and activity of the nitrogen-fixing bacteria at high temperature are remarkably improved, and meanwhile, the functions of intelligent nutrient release and magnetic recovery are achieved. The fertilizer is prepared from composite microcapsules, amino acid, humic acid and other raw materials according to a specific ratio through magnetic field orientation treatment, multi-stage mixing, atomization granulation and magnetic field screening processes, the particle size is controlled to be 0.5-3.35 mm, and the shelf life is prolonged to 12 months. The method has the technical effects that trehalose-glycerol / sorbitol synergistically forms a glassy protective film to maintain the stability of thalli, gamma-aminobutyric acid activates nitrogenase gene expression, magnetic nanoparticles construct a photosynthetic symbiotic system to optimize rhizosphere oxygen supply, and silanized FeO nanoparticles fix heavy metal ions through electrostatic adsorption, so that the heavy metal ions in the thalli are effectively adsorbed, and the heavy metal ions in the thalli are effectively adsorbed. And the humic acid buffers the pH fluctuation of the soil, so that the nitrogen fixation efficiency is finally improved by 30-50%, and the crop yield is increased by 15-25%.

The invention discloses an electrotransfection buffer solution and application of the electrotransfection buffer solution in exosome loaded polypeptide, and relates to the technical field of biology. The electrotransfection buffer solution is prepared from the following components: 80 to 120 mM of trehalose, 6 to 10 mM of dipotassium phosphate, 12 to 16 percent of iodixanol and a buffer solution. The invention also provides a method for carrying out exosome drug loading by using the electrotransfection buffer solution, more efficient loading of palmitoyl pentapeptide-4 by the exosome is realized, the loading capacity of molecules is improved on the premise of not losing the exosome, a higher collagen expression level can be obtained, and the method disclosed by the invention can be applied to large-scale and large-scale production. And the subsequent yield requirement is met.

The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-HER2 antibody, such as e.g. Trastuzumab (HERCEPTIN™), Pertuzumab or T-DM1, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-HER2 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. The formulations comprise additionally at least one buffering agent, such as e.g. a histidine buffer, a stabilizer or a mixture of two or more stabilizers (e.g. a saccharide, such as e.g. α,α-trehalose dihydrate or sucrose, and optionally methionine as a second stabilizer), a nonionic surfactant and an effective amount of at least one hyaluronidase enzyme. Methods for preparing such formulations and their uses thereof are also provided.

The invention discloses an acne-removing essence and a preparation method thereof, and particularly relates to the technical field of skin care products, the acne-removing essence comprises the following components by mass: 0.5-5% of salicylic acid; 1%-5% of tartaric acid; 2%-6% of nicotinamide; 0.5%-2% of colloidal sulfur; 1.5%-3% of tea tree essential oil; 1%-2% of hyaluronic acid; 0.5%-1% of ceramide; 1%-3% of glycerol; 1%-2% of trehalose; the salicylic acid is used for penetrating into skin, helping to relieve dead skin cells, reducing inflammation and accelerating skin renewal; the tartaric acid is used for realizing a stripping effect, can remove aged cuticles on the surface of the skin and promote the generation of new cells, so that the problem of acnes is helped to be improved. The acne-removing essence prepared by the invention has a relatively good moisturizing effect, so that skin barrier can be well maintained, keratin metabolism is promoted, grease secretion is inhibited, acne is effectively prevented, inflammatory response of the acne is relieved, and the actual use effect of the acne-removing essence is relatively good.

The invention provides cytokine hydrogel as well as a preparation method and application thereof in skin injury repair, and belongs to the technical field of biological medicines. Comprising the following steps: (1) adding beta-GP into a chitosan solution to obtain a CS-beta-GP pregel solution; (2) dissolving Pluronic F127 and hyaluronic acid in a PBS (Phosphate Buffer Solution) until the Pluronic F127 and the hyaluronic acid are completely transparent to obtain an F127-HA solution; and (3) sequentially dissolving trehalose, cordycepin, a cell factor composition and collagen peptide in a PBS solution, incubating to obtain a cell factor mixed solution, and mixing the cell factor mixed solution with the CS-beta-GP pregel solution and the F127-HA solution. According to the invention, a dual temperature-sensitive system is established, the gel can be quickly formed under the conditions of injection at low temperature and body temperature, the effective period of active ingredients is prolonged by utilizing a trehalose stabilization technology, and meanwhile, the anti-inflammatory effect of cordycepin and the cell repair effect of collagen peptide are combined, so that the efficient repair of skin injury can be realized.

The disclosure provides for cryopreservation formulations, cryopreservation media and method of cryopreserving mammalian cells or tissues. The cryopreservation formulations and media contain a combination of cryoprotective agents selected from the group consisting of; (a) glycerol and trehalose; (b) glycerol, trehalose, and ascorbic acid; (c) glycerol, trehalose, and taurine: and (d) glycerol, trehalose, ascorbic acid, and taurine. In certain embodiments, the cryopreservation formulations and media are free of dimethyl sulfoxide.