3087 results about "Trehalose" patented technology

Yogurt containing lactic acid bacteria and Bifidobacteria, to which one or more kinds of sweet substances selected from a group consisting of palatinose, palatinite, maltose, maltitol, starch syrup, reducing starch syrup and trehalose as well as a refined fish oil containing highly unsaturated fatty acids such as DHA and EPA are added, is packed in an oxygen blocking hermetic package. The yogurt contains highly unsaturated fatty acids and has good flavor and taste, as well as excellent product quality stability, and does not generate a fishy odor during normal storage periods.

A disintegrant assisted controlled release device is disclosed. The device is a combination of a swelling disintegrant or super-disintegrant and water insoluble polymer or water soluble polymer, or both, and one or more water soluble or water insoluble active pharmaceutical ingredient(s). The said device is stabilized by a humectant or trehalose.

The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-CD20 antibody, such as e.g. Rituximab, Ocrelizumab, or HuMab<CD20>, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-CD20 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. The said formulations comprise additionally at least one buffering agent, such as e.g. a histidine buffer, a stabilizer or a mixture of two or more stabilizers (e.g. a saccharide, such as e.g. α,α-trehalose dihydrate or sucrose, and optionally methionine as a second stabilizer), a nonionic surfactant and an effective amount of at least one hyaluronidase enzyme. Methods for preparing such formulations and their uses thereof are also provided.

The present invention is organ and tissue preservation solutions that provide improved viability of an organ such as a heart or lung, or portion of the organ, for storage and transplantation. In particular, a solution contains trehalose, magnesium sulfate, calcium chloride, heparin, dextran, nitroglycerin, adenosine, L-arginine, allopurinol, reduced glutathione, db-cylic AMP and potassium phosphate.

The present invention has an object to provide a means for durably exerting an action of enhancing collagen-production, and the object is attained by providing a collagen-production enhancer which contains, as an effective ingredient, α,α-trehalose and / or a saccharide derivative thereof, and by a composition incorporated with the collagen-production enhancer to be used for such purpose.

Novel trehalose click polymers have in vitro and in vivo application in the cellular delivery of biologically active molecules, including nucleic acids and polypeptides. The trehalose click polymers of the present invention provide increased stability in serum as compared with other non-viral vectors, and are particularly useful in protecting nucleic acids against degradation.

The invention provides a cell cryo-preserved liquid, an application, and an immune cell cryo-preservation method. The cell cryo-preserved liquid includes 0.7-0.9 ml / ml of a cell culture medium, 8-26 mg / ml of non-essential amino acids, 0.04-0.1 ml / ml of trehalose, 0.5-10 mg / ml of vitamin C, 10-50 mg / ml of human albumin, 0.04-0.12 ml / ml of propylene glycol, 0.01-0.07 ml / ml of dimethyl sulfoxide and 0.5-3 mg / ml of lentinan. Compared with a cell cryo-preserved liquid in the prior art, the human albumin can replace fetal calf serum to achieve the effects of the fetal calf serum in the cell cryo-preserved liquid, and meanwhile, the cell cryo-preserved liquid is added with the non-essential amino acids, the vitamin C and the lentinan, thereby ensuring cell proliferation vitality after recovery is not influenced. The trehalose and the propylene glycol can replace a protective effect of dimethyl sulfoxide, thereby ensuring moisture in cells to be not crystallized during approach of freezing point. The components are cooperated with each other to achieve cryo-preservation of immune cells.

The present invention relates to a method and composition for the cryopreservation of adipose tissue with the intention to use this tissue in the culturing of stem and / or progenitor cells. The method uses a specific cryoprotection medium to prevent damage of the original tissue during the cryopreservation while still maintaining a high viability of the stem and / or progenitor cells obtained from the cryopreserved adipose tissue. Furthermore the cryoprotection medium of the present invention does not contain any kind of xenogeneic sera, a critical factor since it is the intention of that the cryopreserved tissue is used for obtaining stem and / or progenitor cells that can be used in medicine. The cryoprotection medium is characterized in that it is a solution of physiological water comprising glycerol and sucrose and / or trehalose and optionally serum albumin.

A method is provided for preserving live bacteria by subjecting an aqueous system containing the growing bacteria to drying without special equipment, in the presence of trehalose with or without the addition of divalent cations as stabilizing agents. Further, a dried composition for preservation of aerobic bacteria in a viable state is provided. The dried composition consists essentially of dried viable aerobic bacteria and an appropriate growth medium. The bacteria and growth medium are initially placed in an aqueous solution of 10 mM to 200 mM trehalose and a divalent cation, and dried at room temperature.

The present invention has an object to provide an external dermatological formulation having satisfactory blood flow-promoting effect, antiinflammatory effect, antibacterial effect, moisturizing effect, whitening effect, UV-absorbing effect, UV-scattering effect, antioxidant effect, hair growing effect, hair nourishing effect, emusifying effect, astringent effect, wrinkle-reducing effect, cell-activating effect and / or transdermal absorption-promoting effect with a satisfactory safety and skin feeling; The object is solved by providing an external dermatological formulation comprising a saccharide derivative of α,α-trehalose and one or more members selected from substances having any one of blood flow-promoting effect, antiinflammatory effect, antibacterial effect, moisturizing effect, whitening effect, UV-absorbing effect, UV-scattering effect, antioxidant effect, hair growing efect, hair nourishing effect, emulsifying effect, astringent effect, wrinkle-reducing effect, cell-activating effect and transdermal absorption-promoting effect.

The present invention generally relates to devices and methods for the preservation of cells using drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of cells within a glass or other non-viscous, non-frozen media. In some embodiments, the invention allows for the preservation of cells at temperatures below the freezing point of water, and in some cases at cryogenic temperatures, without inducing ice formation. The cells, in certain embodiments, may be preserved in the presence of intracellular and / or extracellular carbohydrates (which may be the same or different), for example, trehalose and sucrose. Carbohydrates may be transported intracellularly by any suitable technique, for example, using microinjection, or through non-microinjected methods such as through pore-forming proteins, electroporation, heat shock, etc. In certain instances, the glass transition temperature of the cells may be raised, e.g., by transporting a carbohydrate intracellularly. In some cases, the cells may be dried and / or stored, for example, in a substantially moisture-saturated environment or a desiccating environment. The cells may also be stored in a vacuum or a partial vacuum. The cells may be protected from oxygen, moisture, and / or light during storage. In certain cases, an inhibitor, such as a cell death inhibitor, a protease inhibitor, an apoptosis inhibitor, and / or an oxidative stress inhibitor may be used during preservation of the cells. The cells may be stored for any length of time, then recovered to a viable state, e.g., through rehydration, for further use.

The invention relates to the field of stem cells, discloses a method for preparing exosome freeze-dried powder of stem cells, and particularly relates to a method for preparing exosome freeze-dried powder of human amniotic mesenchymal stem cells. The method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention comprises the following steps: mixing trehalose with exosomes of the human amniotic mesenchymal stem cells as a cryoprotectant; filtering and removing bacteria by virtue of a filter membrane; and firstly carrying out ultralow temperature pre-freezing, and then carrying out low-temperature freezing at a certain vacuum degree. The preparation method disclosed by the invention is simple to operate, safe and effective. An experiment proves that compared with a conventional preparation method of the freeze-dried powder, the morphologies and the activity of the exosomes of the human amniotic mesenchymal stem cells can be well kept by the method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention; and the method is suitable for long-term preservation and application of the exosomes of the human amniotic mesenchymal stem cells.

The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-HER2 antibody, such as e.g. Trastuzumab (HERCEPTIN™), Pertuzumab or T-DM1, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-HER2 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. The formulations comprise additionally at least one buffering agent, such as e.g. a histidine buffer, a stabilizer or a mixture of two or more stabilizers (e.g. a saccharide, such as e.g. α,α-trehalose dihydrate or sucrose, and optionally methionine as a second stabilizer), a nonionic surfactant and an effective amount of at least one hyaluronidase enzyme. Methods for preparing such formulations and their uses thereof are also provided.

The present invention has an object to provide an external dermatological formulation having satisfactory blood flow-promoting effect, antiinflammatory effect, antibacterial effect, moisturizing effect, whitening effect, UV-absorbing effect, UV-scattering effect, antioxidant effect, hair growing effect, hair nourishing effect, emusifying effect, astringent effect, wrinkle-reducing effect, cell-activating effect and / or transdermal absorption-promoting effect with a satisfactory safety and skin feeling; The object is solved by providing an external dermatological formulation comprising a saccharide derivative of alpha , alpha -trehalose and one or more members selected from substances having any one of blood flow-promoting effect , antiinflammatory effect, antibacterial effect, moisturizing effect, whitening effect, UV-absorbing effect, UV-scattering effect, antioxidant effect, hair growing efect, hair nourishing effect, emulsifying effect, astringent effect, wrinkle-reducing effect, cell-activating effect and transdermal absorption-promoting effect.

The invention discloses a complex microbial agent for producing soybean paste in Pixian County, which mainly comprises coculture of saccharomyces cerevisiae, candida utilis, lactobacillus plantarum and salt tolerant tetrads, and skim milk powder, glycerin, maltodextrin, trehalose and L-sodium glutamate, or mainly comprises coculture of saccharomyces cerevisiae, candida utilis, lactobacillus plantarum and salt tolerant tetrads, and porous starch, maltodextrin and polyvinyl pyrrolidone. A method for preparing the complex microbial agent for producing soybean paste in Pixian County comprises the following technological steps of: (1) activating and propagating strains; (2) inoculating; (3) co-culturing; and (4) adding a protective agent and drying. By using the complex microbial agent in the preparation of cooked soybean paste, the complex microbial agent maintains the characteristics of traditional soybean paste in Pixian County. Meanwhile, compared with the traditional technology, the complex microbial agent has the advantages of shortening the production period by 1 / 3, improving the amino nitrogen content by 3 to 8 times, improving the volatile aroma content by 2 to 4 times, and ensuring the aflatoxin B1 of 0 to 0.5ppm only.

The present invention discloses a kind of frozen tilapia mossambica fish fillets containing freezing protective agent trehalose and its processing method. Said processing method includes the following steps: making the tilapia mossambica fish undergo the processes of cleaning dressing, boning, skinning and filleting treatments, then adding protective agent to treat fish fillets, taking out said fish fillets, draining to remove excess solution and freezing below-18 deg.C so as to obtain the invented frozen tilapia mossambica fish fillets. The described protective agent is an aqueous solution containing trehalose, glycine and sodium chloride.

Conversion of renewable hydrocarbons to transportation fuels is required to reduce carbon emission, limit the use of fossil fuels, and develop renewable energy sources. Sorbitol, xylitol and trehelose are polyalcohols generated from the liquefaction of various sugars and carbohydrates in biomass from algae, corn, sugarcane, switchgrasses, and biological wastes. Mixtures of aqueous polyols and fuel feedstocks are catalyzed over metal catalysts to produce hexanes, pentanes, and lighter hydrocarbons. By managing the catalyst, reaction conditions and sulfur content, the octane value of the product fuel is dramatically increased.

The invention discloses a functional composition with functions of controlling blood sugar and enhancing nutrition, which is prepared from main materials and a common carrier, wherein the main materials consist of functional sugars, composite vitamins, mineral substance, dietary fiber and high sweetener; and the functional sugars comprise L-arabinose and trehalose, the weight content of the L-arabinose is 10 to 80 percent, and the weight content of the trehalose is 10 to 50 percent. The functional composition uses two functional sugars as main sugar-reducing substances and is added with multiple composite vitamins, the mineral substance, the dietary fiber and the high sweetener, so the invention provides the functional composition with convenient use, good mouthfeel, safety, effectiveness and practicability of controlling blood sugar and enhancing nutrition for diabetes patients. The functional composition can well supplement nutrient substances required for the human body when the diabetes patients can feel relieved to enjoy a proper amount of sweet food.

The invention discloses a Process for the purification and separation of trehalose, containing enzymolysis reaction which adds saccharify enzyme in enzyme reaction liquor which is getted from the produced trehalose by enzymic conversion method and inoculating 3-10wt% Saccharomyces cerevisiae in saccharify enzyme enzymolysis liquor, fermenting and culturing in the condition of 25-30deg C and pH 5. 5-7. 5, then getting trehalose pure product after centrifuging, hyperfiltration, ion-exchange, condensation, crystallization and desiccation. Comparing with prevenient activated charcoal column chromatography and organolite process, this invention using two-stage remove mixed sugar of enzymolysis liquor, detecting the getted trehalose pure product by HPLC, its purity is as high as 98. 0%. at the same time, this invention has the advantage of high detaching and purifying efficiency, few lose of trehalose, low cost, simple technology and so on, bringing active impel on predigesting productive technology, improving production and reducing production costs of trehalose's produce by enzyme method.

The invention discloses a fresh shrimp freezing and antistaling agent as well as a fresh shrimp freezing and antistaling method. The antistaling agent is a mixture containing tea polyphenol, chitosan, antibacterial peptide, nisin, glucose oxidase, sodium chloride, lactic acid and trehalose. The antistaling agent is used for freezing and fresh-keeping of fresh shrimps; the fresh shrimp freezing and antistaling agent has natural components, and can be used for better protecting freshness of aquatic products, maintaining original appearance and state, reducing loss of proteins and other nutrients, inhibiting generation of bacteria, and substantially prolonging shelf-life and storage life.

A nutritional composition for controlling blood sugar level comprising a protein, a lipid and a carbohydrate, wherein energy percentages supplied by the protein, lipid and carbohydrate are 10 to 25%, 20 to 35% and 40 to 60%, respectively; and oleic acid in the lipid energy percentage is 60 to 90% and palatinose and / or trehalulose in the carbohydrate energy percentage is 60 to 100%. The composition is useful as an oral or tube feeding nutrient for nutritional management or blood sugar level control of patients suffering from diabetes and glucose intolerance, or for obesity prevention, a therapeutic diet, a diet for diabetic patients at home, an obesity preventive diet or a food with health claims.

A stable blood factor composition contains a stabilising amount of trehalose in the absence of human serum albumin to provide a product stable at up to 60° C.

The invention relates to gum-protecting whitening toothpaste. The gum-protecting whitening toothpaste is prepared from the following components: sorbitol, xylitol, sodium phytate, brilliant blue pigment, sodium lauroyl sarcosine, ethylene diamine tetraacetic acid (EDTA) disodium, plant chitosan, trehalose, sodium saccharin, sodium pyrophosphate, glycerol, sodium hyaluronate, xanthan gum, hydroxyethyl cellulose, polyvinyl pyrrolidone (PVP) cross-linking agent, shell powder, silicon dioxide (friction type), silicon dioxide (thickening type), zinc citrate, lysozyme, pawpaw extract, vitamin E, peppermint essence, silica (whitening particles) and pure water. The components coordinate with one another, so that the effects of protecting gum and whitening teeth are achieved.

The invention relates to a composition used as a vaccine freeze-drying protective agent, comprising the components with initial concentration in the freeze-drying stock solution as follows: 30-100g / l sucrose, 10-30g / l trehalose, 10-50g / l dextran, 6-12g / l sodium glutamate, 3-9g / l urea and 0.5-2g / l arginine, and no gelatin. The invention further provides a method for preparing freeze-drying vaccines by using the freeze-drying protective agent and application of the freeze-drying protective agent for improving the stability of freeze-drying vaccines.

The invention discloses a mask solution. The mask solution contains a moisturizer, wherein the moisturizer is prepared from sodium hyaluronate, trehalose, sodium polyglutamate, betaine, tremella polysaccharide and glyceryl polymethacrylate. The mask solution disclosed by the invention is capable of durably and deeply moisturizing the skin, is good in mildness, is also suitable for skin sensitive persons, is natural and non-toxic, is mild and non-irritant, cannot damage the skin after long-term use, has an obvious effect in improving the skin, and is beneficial for enabling the skin to restore elasticity and be glossy.

The invention relates to a composite soil conditioner, comprising biogas residues, pyroligneous acid, mineral powder, soluble chitosan, diethylene triaminepentaacetic acid pentasodium, a surfactant, tobacco powder, trehalose, di-rhamnolipid, a flocculating component, a penetrating agent, enteromorpha powder, modified zeolite, boron-zinc-magnesium-containing fungicide, a nano additive, an accelerant, pentaerythritol triacrylate and ionic liquid, preferably also comprising 4-methyl phenyl valerate which is equal to the ionic liquid in weight. The novel composite soil conditioner has excellent soil improvement and recovery performance, the utilization rate of the fertilizer by the crop is greatly increased, and the composite soil conditioner can significantly increase the yield, simultaneously also can improve the permeability of the soil and avoids side effects of heavy metals such as cadmium; thus, the composite soil conditioner is beneficial to crop growth and has a wide industrial application prospect.

The invention discloses a cryoprotective agent of peripheral blood mononuclear cells. The cryoprotective agent is prepared from the following ingredients in parts by weight: 43-53 parts of a culture medium, 30-40 parts of auto-plasma, 12-17 parts of dimethyl sulfoxide, 5-7% of human serum albumin, and the balance being 0.17-0.23mol / L trehalose. The invention discloses a preservation method of the cryoprotective agent. The preservation method comprises the steps of collecting fresh anticoagulation blood, preparing peripheral mononuclear cells into cell suspension by using human serum albumin, adding the cryoprotective agent with the same volume as that of the cell suspension, mixing uniformly, preserving for 10-20h at below 90-below-70-below DEG C, and preserving in liquid nitrogen. According to the cryoprotective agent and the preservation method thereof, a cryopreserved cell has the high motility rate and the strong cell activity and can shorten the Cytokine Induced Killer (CIT) cell induced amplification period.

The invention discloses a method of using waste bear yeast to simultaneously prepare zymosan, trehalose and yeast extract. The method comprises steps of debittering and impurities removing of the waste bear yeast, autolysis, enzymatic hydrolysis and inactivation of the waste bear yeast, separation and drying of the zymosan, membrane separation, concentration, crystallization and drying of the trehalose, concentration and drying of the yeast extract, etc. The method has the characteristics of high raw material utilization rate, good quality, low cost and easy industrialization, realizes that three products of the zymosan, the trehalose and the yeast extract are simultaneously produced from the same industrial waste, the waste bear yeast, improves the utilization rate of industrial wastes and achieves the target of maximal waste resource recovery.

The invention relates to a recombinant human basic fibroblast growth factor gelling agent and the process for preparation, which comprises recombinant human basic fibroblast growth factor (rh-bFGF) stock solution 0.1-100% (W / W), medicinal auxiliary material 0-99.9% (W / W), the stock solution comprises effective treatment dose of recombinant human basic fibroblast growth factor (rh-bFGF) 0.01-1% (ug / g), NaCl 0.01-20% (W / W), phosphates cushioning liquid 2m Mol - 200m Mol, and right amount of water for injection, the medicinal auxiliary materials can be selected from: carbomer 940NF, sodium hyaluronate, trehalose, mannitol, dextran 40 and sodium heparin.

The invention discloses a mesenchymal stem cell frozen stock solution which does not contain animal proteins and DMSO (dimethyl sulfoxide). The frozen stock solution comprises trehaloses, a carbohydrate and electrolyte injection, ATP (adenosine triphosphate) and human serum albumins. The frozen stock solution has the advantages of high recovery rate (90%), no influence on characteristics of stem cells, unchanged cell proliferation, unchanged cell differentiation, easy use, good stability and the like. The invention also discloses a preparation method of the frozen stock solution.