1308 results about "Trehalose" patented technology

The invention belongs to the technical field of biology, and discloses a stable protective agent, a cell antigen dry sheet and a preparation method and application of the dry sheet, the stable protective agent comprises glycerin with the volume fraction being 0.4%, gelatin with the mass volume fraction being 0.1%, EDTA with the mass volume fraction being 0.1 mM, trehalose with the mass volume fraction being 0.03% and resveratrol with the mass volume fraction being 100 [mu] M. The stable protective agent is adopted for preparing the cell antigen dry sheet, and the cell antigen dry sheet is applied to CBA detection. The stable protective agent has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent cell deformation and cell fixing layer shedding, and can well maintain the stability and reliability of antigen proteins, and the prepared cell antigen dry sheet can be stored for a long time, and has a good application prospect. The antigenicity of a membrane signal index is not damaged. When a positive sample is detected, obvious green fluorescence cells can be seen, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed conveniently.

The invention relates to the field of skin care and medical cosmetology, and discloses a long-acting moisturizing and barrier repairing type exosome active gel and a preparation method thereof, and the gel comprises the following components by mass: 2-5 parts of exosome; 3 to 7 parts of trehalose; 2 to 6 parts of glycerol; 1 to 2 parts of polyvinyl acrylate; 0.5 to 1.5 parts of octyl glucoside; 0.8 to 1.5 parts of sodium hyaluronate; 0.2 to 0.5 part of low molecular peptide; 1 to 3 parts of polyacrylamide; and 0.02 to 0.05 part of a tea polyphenol antioxidant. According to the invention, through synergistic repair of the exosome and the low-molecular peptide, dual moisturizing of the trehalose and the glycerol, a thickening system is innovated to improve the stability, and the tea polyphenol antioxidant is combined, so that deep repair, lasting moisturizing, skin feeling optimization and aging resistance are effectively realized, and multiple bottleneck problems of a traditional skin care technology are comprehensively solved.

The invention relates to a concrete anti-cracking self-repairing agent in the technical field of building materials, and aims to solve the problem that concrete cracks are difficult to self-repair. According to the technical scheme, the self-repairing agent is characterized by comprising a microbial repairing unit formed by ZSM-5 type zeolite molecular sieve loaded bacillus pasteurii spores, mesoporous silica particles loaded with L-calcium aspartate and Fe3O4 (at) SiO2 core-shell nanoparticles which are fixed through an in-situ polymerization coating layer to form a magnetic thermal response capsule layer coating the surface of the microbial repairing unit, pLGA microspheres containing yeast extract are positioned on an interface of zeolite and hydrogel, and a trehalose-anhydrous calcium chloride eutectic compound is used as a directional mineralization accelerant. The concrete anti-cracking self-repairing agent provided by the invention is mainly used for intelligent identification and self-repairing of concrete cracks, and efficient repairing is realized through a microbial mineralization, calcium ion slow release and magnetic thermal response synergistic mechanism.

The invention relates to a functional microorganism-containing organic-inorganic compound fertilizer and a production method thereof, and relates to the technical field of fertilizers, the functional microorganism-containing organic-inorganic compound fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer, the functional microorganism outer layer comprises the following components in parts by mass: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of a yeast extract and 30-40 parts of a modified activated carbon carrier. The production method comprises the following steps: preparing an inorganic core layer, preheating the inorganic core layer, and spraying and coating an organic coating layer material to obtain a primary product with an organic coating layer; and coating the surface of the organic coating layer of the primary product with a functional microorganism outer layer material, drying at low temperature, and screening to obtain the organic-inorganic compound fertilizer containing the functional microorganisms. The fertilizer has the effect of improving the slow release effect of the fertilizer and the survival rate of microorganisms.

The invention relates to the field of nutrient solutions, and particularly discloses a high-calcium-zinc dietary nutrient solution for promoting skeletal development and a preparation method thereof, the high-calcium-zinc dietary nutrient solution for promoting skeletal development comprises the following raw materials: calcium citrate, zinc citrate, a zinc-containing yeast extract, vitamin D3, a nutrition enhancer, functional oligosaccharide, xanthan gum, anhydrous citric acid, essence, a sweetening agent and water; the preparation method comprises the following steps: mixing the zinc citrate, the zinc-containing yeast extract, the seaweed meal, the natto powder, the stachyose and the trehalose to prepare a mixture A; uniformly premixing stevioside, xanthan gum and trehalose to obtain a mixture B; s4, vitamin D3 powder and trehalose are premixed and dissolved, and a mixture C is prepared; heating the residual water, adding the mixture A, the fructo-oligosaccharide, the anhydrous citric acid, the calcium citrate, the mixture B, the trehalose and the mixture C, adding the essence, and stirring to obtain the beverage. The preparation method has the characteristic of preparing the liquid calcium-zinc dietary nutrient solution with good absorption and utilization rate and palatability.

The invention relates to preparation of high-stability mascarpone cheese and application of the mascarpone cheese in baking, and belongs to the technical field of cheese preparation. The glutamine transaminase is combined with a citric acid-sodium citrate system, so that the excessive denaturation of protein is reduced, and the texture and flavor of the cheese are improved. Trehalose and xanthan gum are added for locking water and resisting freezing, and an antioxidant is combined for use, so that the freeze-thaw stability and oxidation resistance of the cheese are improved, the cheese is protected from texture change and oxidative damage in the freezing and unfreezing processes, and excellent texture and flavor can still be kept after multiple times of circulation. Arabic gum is modified through octenyl succinic anhydride, amphipathy is obtained, whey separation is prevented, whey loss is reduced, and the cheese taste is improved.

The application relates to an organic-inorganic compound fertilizer containing functional microorganisms and a production method thereof, and relates to the technical field of fertilizers. The fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer. The functional microorganism outer layer comprises the following components in mass fractions: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of yeast extract and 30-40 parts of modified active carbon carriers. The production method comprises the following steps: preparing the inorganic core layer, preheating the inorganic core layer, spraying the organic coating layer material to coat the surface, and obtaining a preliminary product with the organic coating layer; coating the functional microorganism outer layer material on the surface of the organic coating layer of the preliminary product, low-temperature drying and screening, and obtaining the organic-inorganic compound fertilizer containing the functional microorganisms. The application has the effects of improving the slow-release effect of the fertilizer and the survival rate of the microorganisms.

The invention relates to a natural antioxidant for prefabricated vegetables and a preparation method of the natural antioxidant, and belongs to the technical field of food additives. The antioxidant is prepared from rosmarinic acid, carnosic acid, drug-loaded cellulose and double-phase chelating microspheres; the drug-loaded cellulose is prepared by modifying cellulose with quercetin, loading anthocyanin and then wrapping with a trehalose-gamma-polyglutamic acid composite film; the biphase chelating microspheres are phytic acid-citric acid biphase sustained-release microspheres formed by wrapping phytic acid and citric acid with a chitosan and sodium alginate composite film. Through multi-component functional complementation, multi-stage slow release design and physical-chemical synergistic barrier, comprehensive inhibition of the oxidation problem of the prefabricated vegetable is realized, a'free radical scavenging-metal chelating-phase interface stabilization 'triple protection system is constructed, accurate oxidation resistance in different stages of processing, storage and transportation is realized, and the prepared vegetable has good market prospects. The bottlenecks of short action period and single protection dimension of a natural antioxidant are broken through.

The invention discloses a composition containing PDRN microspheres as well as a preparation method and application thereof, and relates to the technical field of repair. The composition containing the PDRN microspheres is prepared from the following components in parts by weight: 1 to 10 parts of the PDRN microspheres, 30 to 60 parts of sodium hyaluronate, 0.05 to 1 part of a cross-linking agent, 0.5 to 5 parts of bisabolol and 1 to 8 parts of trehalose, the molecular weight of the sodium hyaluronate is 300 kDa to 800 kDa. By adding the PDRN microspheres and selecting reasonable component compatibility, the stability is remarkably improved, and a synergistic effect is reflected in the aspect of repairing.

The invention belongs to the technical field of stem cell biological preparations, and particularly relates to an anti-aging compound preparation based on stem cells and application of the anti-aging compound preparation in delaying skin aging. The composition is composed of an inner water phase, a middle oil phase and an outer water phase, wherein the inner water phase is prepared from the following components: stem cell exosomes, hyaluronic acid, nicotinamide ribose, trehalose, mannitol, glycine and a citric acid buffer solution; the medium oil phase is prepared from the following components: medium chain triglyceride, shea butter, vitamin E succinate, PEG (Polyethylene Glycol) modified ceramide NP and cholesterol succinic acid monoester; the external water phase is prepared from the following components: polydatin, cellulose nanocrystals and an emulsifying agent. On the basis of a W / O / W emulsion system, a multi-layer anti-aging mechanism is constructed by utilizing a three-dimensional delivery framework of an inner water phase, an oil phase and an outer water phase and space-time synergy of active ingredients, the exosome permeation efficiency is improved through microneedle introduction, and the anti-oxidation and barrier repair effects are achieved by using multiple ingredients.

The invention discloses a diluent for whole blood lateral immunochromatography detection as well as a preparation method and a detection method thereof, and belongs to the technical field of immunochromatography detection. The diluent solves the technical problems of unsmooth chromatography, background interference, poor stability of detection results and the like caused by red blood cells during direct detection of whole blood samples. The diluent comprises a buffer system, an osmotic pressure regulator glycine, a nonionic surfactant polysorbate, an erythrocyte aggregation inducer polyvinylpyrrolidone, a dynamic viscosity regulator trehalose, a blocking protein, a stabilizer combination L-methionine and L-histidine, and a composite formula of water. Red blood cells are effectively induced to gather and intercept on the sample pad through the synergistic effect of all the components, and meanwhile the stability of the chromatography process and the activity of the detection reagent are maintained. The diluent is mainly used for lateral immunochromatography quantitative detection of markers such as pro-enkephalin, C-reactive protein, cardiac troponin I or N-terminal pro-brain natriuretic peptide in a whole blood sample.

The invention discloses a recombinant III-type human collagen composition. The recombinant III-type human collagen composition comprises recombinant III-type human collagen, a first buffer solution and a stabilizer, the stabilizer is selected from at least one of glycerol, butanediol, pentanediol, mannitol and trehalose. According to the recombinant III type human collagen composition, a chemical cross-linking agent or an enzymatic promoter is not added for cross-linking, and the used reagents are free of cytotoxicity, sensitization and other safety risks. Therefore, the recombinant III-type human collagen composition has no crosslinking agent residue, can form a three-dimensional network structure, can avoid the problem that the recombinant III-type human collagen and the polysaccharide are compounded to generate precipitates, also has good biocompatibility, degradability and high stability, especially has relatively high thermal stability, and can be used for preparing the recombinant III-type human collagen composition. The wound healing and repairing can be promoted, the healthy skin ecology can be reconstructed, and a new choice is provided for a biomedical material.

According to the high-temperature-resistant nitrogen-fixing bacteria microcapsule fertilizer and the preparation method thereof, aiming at the problems of thallus inactivation and nutrient loss in a high-temperature environment, a sodium alginate-chitosan double-layer embedding technology is combined with magnetic nanoparticle modification, and a stable double-layer structure is formed by optimizing the ratio of an embedding material, so that the high-temperature-resistant nitrogen-fixing bacteria microcapsule fertilizer is prepared. The survival rate and activity of the nitrogen-fixing bacteria at high temperature are remarkably improved, and meanwhile, the functions of intelligent nutrient release and magnetic recovery are achieved. The fertilizer is prepared from composite microcapsules, amino acid, humic acid and other raw materials according to a specific ratio through magnetic field orientation treatment, multi-stage mixing, atomization granulation and magnetic field screening processes, the particle size is controlled to be 0.5-3.35 mm, and the shelf life is prolonged to 12 months. The method has the technical effects that trehalose-glycerol / sorbitol synergistically forms a glassy protective film to maintain the stability of thalli, gamma-aminobutyric acid activates nitrogenase gene expression, magnetic nanoparticles construct a photosynthetic symbiotic system to optimize rhizosphere oxygen supply, and silanized FeO nanoparticles fix heavy metal ions through electrostatic adsorption, so that the heavy metal ions in the thalli are effectively adsorbed, and the heavy metal ions in the thalli are effectively adsorbed. And the humic acid buffers the pH fluctuation of the soil, so that the nitrogen fixation efficiency is finally improved by 30-50%, and the crop yield is increased by 15-25%.

The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-HER2 antibody, such as e.g. Trastuzumab (HERCEPTIN™), Pertuzumab or T-DM1, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-HER2 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. The formulations comprise additionally at least one buffering agent, such as e.g. a histidine buffer, a stabilizer or a mixture of two or more stabilizers (e.g. a saccharide, such as e.g. α,α-trehalose dihydrate or sucrose, and optionally methionine as a second stabilizer), a nonionic surfactant and an effective amount of at least one hyaluronidase enzyme. Methods for preparing such formulations and their uses thereof are also provided.

The invention provides cytokine hydrogel as well as a preparation method and application thereof in skin injury repair, and belongs to the technical field of biological medicines. Comprising the following steps: (1) adding beta-GP into a chitosan solution to obtain a CS-beta-GP pregel solution; (2) dissolving Pluronic F127 and hyaluronic acid in a PBS (Phosphate Buffer Solution) until the Pluronic F127 and the hyaluronic acid are completely transparent to obtain an F127-HA solution; and (3) sequentially dissolving trehalose, cordycepin, a cell factor composition and collagen peptide in a PBS solution, incubating to obtain a cell factor mixed solution, and mixing the cell factor mixed solution with the CS-beta-GP pregel solution and the F127-HA solution. According to the invention, a dual temperature-sensitive system is established, the gel can be quickly formed under the conditions of injection at low temperature and body temperature, the effective period of active ingredients is prolonged by utilizing a trehalose stabilization technology, and meanwhile, the anti-inflammatory effect of cordycepin and the cell repair effect of collagen peptide are combined, so that the efficient repair of skin injury can be realized.

The invention discloses a biological challenge device with combined adjustable resistance for evaluating the effect of a sterilization process, which comprises a shell, the shell comprises a plastic pipe and an inner pipe cap detachably connected with the inner wall of the plastic pipe, the inner pipe cap is provided with a breathable window and covered by a breathable film, and a bacterial contamination carrier for bearing indicator biological spores is attached to the inner bottom of the plastic pipe. An openable container containing a liquid culture medium is arranged in the plastic pipe cavity; the outer side of the inner pipe cap is sleeved with an outer pipe cap detachably connected with the outer wall of the plastic pipe, and the outer pipe cap is provided with a flow blocking structure used for adjusting the speed of the sterilizing agent entering the area of the inner pipe cap; the indicator biological spores are pretreated by a stable protective agent containing one or more of polyethylene glycol, trehalose, sorbitol, polyvinylpyrrolidone and glyceryl stearate. The speed of a sterilizing agent entering the device can be limited, the spores are dry in a suspension added with a stable protective agent, the capability of resisting general environmental changes is very good, and the performance of the device can be maintained for a long time.

The invention relates to the technical field of fertilizers and pesticides for agricultural production, and discloses a liquid pesticide fertilizer for preventing and treating root-knot nematodes in a water-fertilizer integrated system and application thereof, and the liquid pesticide fertilizer is prepared from the following components: a plant source nematicide, a biocontrol inoculant, a macroelement and medium trace element fertilizer, a boron cross-linking agent, a modified polycarboxylic acid dispersing agent, a microbial protective agent and the like. The preparation method comprises the steps of biological phase pretreatment, matrix phase construction, oil phase micro-emulsification, multiphase fusion and dynamic crosslinking. Through trehalose gradient permeation pretreatment, the survival rate of biocontrol bacteria in a high-salt matrix is increased; a boric acid ester bond dynamic cross-linked network is constructed by using a variable pH adjusting process, so that the system is endowed with shear thinning characteristics, and the contradiction between suspension stability and drip irrigation fluidity is solved; and the chelating dispersion effect of the modified polycarboxylic acid dispersing agent is matched, so that the generation of precipitates is effectively inhibited, and stable coexistence and synergistic interaction of the pesticide, the fertilizer and the bacteria are realized.

The invention relates to the technical field of cosmetics, and discloses a cosmetic composition, a preparation method and application of the cosmetic composition in anti-aging and anti-wrinkle skin. The preparation method comprises the following steps: expressing SKAP2 protein through prokaryote escherichia coli, extracting milk exosome, wrapping the SKAP2 protein into the milk exosome, and mixing the milk exosome containing the SKAP2 protein with trehalose dihydrate, water and CMC (Carboxymethyl Cellulose) to prepare a hydrogel preparation which can be used as a cosmetic composition applied to gel coating agents, masks, toner and dry powder patches. The anti-wrinkle effect is achieved by inhibiting the activity of matrix metalloproteinase 1 (MMP1).

The invention discloses an efficient extraction method of large-molecular-weight astragalus polysaccharide, which belongs to the technical field of plant extraction, and comprises the following steps: cleaning astragalus, filtering, and crushing; the preparation method comprises the following steps: mixing a beta-1, 3-glucanase aqueous solution and trehalose ethylene diamine, and stirring for 10-30 minutes to obtain an enzyme protection system; soaking the radix astragali powder in a citric acid solution, adding an enzyme protection system, applying a bipolar pulsed electric field, and reacting at 40-60 DEG C for 30-40 minutes; adjusting the concentration of the ethanol to 40-50%, standing, centrifuging and collecting crude astragalus polysaccharide; purifying, decolorizing, concentrating, freeze-drying and crushing the crude astragalus polysaccharide to obtain refined astragalus polysaccharide; the mass ratio of the trehalose ethylene diamine to the beta-1, 3-glucanase is (15: 1) to (30: 1). The enzyme protection system maintains the activity of the enzyme, the enzyme and the pulsed electric field act synergistically, the extraction time is shortened, and the extraction rate is increased while the astragalus polysaccharide with large molecular weight is obtained.

The invention provides an anti-freezing denaturation agent for golden and tiger hybrid spotted fish meat protein and a preparation method of the anti-freezing denaturation agent. The invention relates to an anti-freezing denaturation agent for protein of Jinhu hybrid spotted fish. The anti-freezing denaturation agent is prepared from the following raw materials in parts by weight: 5 to 8 parts of trehalose-mannitol, 0.5 to 1 part of nano chitosan, 0.2 to 0.5 part of rosmarinic acid and 0.1 to 0.2 part of polysorbate-80. The trehalose-mannitol is prepared from trehalose and mannitol. Aiming at the characteristics of Jinhu hybrid spotted fish meat, trehalose-mannitol, nano chitosan, rosmarinic acid and polysorbate-80 are taken as raw materials and compounded to obtain a new formula of the protein anti-freezing denaturation agent for the Jinhu hybrid spotted fish meat, and the anti-freezing denaturation agent prepared by adopting the formula and the process disclosed by the invention can be used for inhibiting protein degradation and improving the anti-freezing denaturation effect of the Jinhu hybrid spotted fish meat. The decrease range of MP protein concentration, MP total sulfydryl content and MP Ca < 2 + >-ATPase activity of the antifreeze agent is obviously reduced, and the effect of the antifreeze agent is superior to that of a commercially available antifreeze agent. Moreover, the anti-freezing denaturation agent for the protein of the Jinhu hybrid spotted fish does not contain phosphorus, and has the advantages of safety and high efficiency.

The invention belongs to the technical field of microorganisms, and discloses a preparation method of a microbial agent and an organic fertilizer. The microbial agent comprises the following raw materials in parts by weight: 10-20 parts of a complex microbial agent, 5-10 parts of an accelerant and 5-10 parts of nutrient substances, the complex microbial inoculants comprise bacillus velezensis, pseudomonas monteilii and lactobacillus pentosus; the accelerant comprises modified saponin. The synergistic prevention and control effect on disease prevention and control when the bacillus velezensis, the pseudomonas monteilii and the lactobacillus pentosus are used in a composite mode is utilized, diseases are greatly reduced, and yield and income are increased. Meanwhile, the surface activity of the modified saponin can promote attachment of microorganisms on the surfaces of soil particles and plant roots, and the activity and utilization efficiency of the microorganisms are improved; the fertilizer can also form a stable compound with nutritional ingredients in the organic fertilizer, so that the loss of nutrients is reduced; trehalose is added to serve as a nutritional ingredient for rapid proliferation of microbial flora.

The invention discloses chocolate sandwich cheese and a preparation method, the chocolate sandwich cheese comprises a cheese sandwich and a chocolate layer, and the chocolate layer is coated on the outer side surface of the cheese sandwich; the cheese sandwich comprises cheese; white granulated sugar; a protein material; trehalose; milk powder; a calcium raw material; an emulsifying salt; and an emulsion stabilizer. The chocolate sandwich cheese prepared from the components is stable in cheese system, free of massive oil and water separation, free of chocolate melting deformation, good in taste and convenient to store and transport.

The invention relates to the field of freeze-drying protective agents, and particularly discloses a vacuum freeze-drying protective agent for lactobacillus plantarum LS-41. The vacuum freeze drying protective agent for the lactobacillus plantarum LS-41 comprises the following components: 15 parts of dried skim milk, 3 parts of cane sugar, 1 part of PBS dry powder, 6-10 parts of trehalose, 0.2-0.4 part of ascorbic acid, 0.06-0.10 part of sodium glutamate, 8-12 parts of sorbitol and 100 parts of ultrapure water. Through the synergistic effect of the trehalose, the ascorbic acid, the sodium glutamate, the sorbitol, the skimmed milk powder and the cane sugar, and improvement and reasonable matching of the category and the concentration of the protective agents, the damage of the lactobacillus plantarum LS-41 in the vacuum freeze-drying process can be reduced, and the freeze-drying survival rate of the lactobacillus plantarum LS-41 is increased.

The invention discloses a cryoprotectant which comprises glycerol, skimmed milk powder, mannitol, trehalose, vitamin C, fructo-oligosaccharide, inulin and sterile water. By optimizing the components of the cryoprotectant, the activity of the coprophilous fungi can be effectively protected, the survival rate of the intestinal coprophilous fungi in the freezing process is remarkably increased, all the used components are food-grade edible components and have a wide application range and economic effects, the invention further discloses a coprophilous fungi freezing preservation method adopting the cryoprotectant, and the cryoprotectant has good application prospects. The method is simple, easy to operate and suitable for large-scale production and application.

The invention discloses a fresh-cut eggplant anti-browning and fresh-keeping method based on composite plant extract synergistic heat treatment, and the method comprises the following steps: S1, preparing a composite plant extract comprising the following components: a green tea extract, a rosemary extract, a licorice extract, trehalose, chitosan oligosaccharide, curcumin microcapsules, soybean lecithin, Tween, and the balance of pure water; s2, the fresh-cut eggplants are soaked in a composite plant extracting solution, gradient heat treatment is carried out, and the gradient heat treatment comprises the following three stages of a heat activation permeation stage, a cooling stabilization stage and a cold shock fixation stage; and S3, slightly draining the treated fresh-cut eggplants, and packaging with a preservative film. Through the synergistic effect of various plant extracts and an elaborately-designed gradient heat treatment process, effective inhibition of browning of the fresh-cut eggplants is achieved, the fresh-keeping period is remarkably prolonged, good quality can be kept for 10 days or above under the condition of 4 + / -1 DEG C, and the fresh-cut eggplant fresh-keeping method has a good application prospect.

The invention belongs to the technical field of exosome freeze-dried powder, and discloses exosome freeze-dried powder and a preparation method thereof, the exosome freeze-dried powder contains mesenchymal stem cell exosome, trehalose, sodium hyaluronate, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide. According to the exosome freeze-dried powder, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide are added, the membrane structure integrity of the exosome is effectively protected, the retention rate of active substances in the exosome freeze-dried powder is high, and therefore the effects of resisting inflammation, regulating cell metabolism, promoting tissue repair and the like of the exosome can be fully exerted. In addition, glutathione can directly remove skin free radicals and inhibit oxidative stress injury, and has an anti-oxidation effect; ceramide can also supplement skin lipid barrier, enhance the water locking capacity and enhance the moisturizing effect of sodium hyaluronate; fructo-oligosaccharide can also regulate skin micro-ecological balance, and has anti-inflammatory and bacteriostatic effects.

The invention relates to the technical field of dairy product fermentation, and discloses a high-activity probiotic microcapsule suitable for dairy product fermentation and a preparation method thereof.The microcapsule is prepared from, by weight, 6.7-13.3 parts of bifidobacterium lactis, 3.3-6.7 parts of lactobacillus plantarum, 9.1-15.2 parts of Arabic gum, 13.6-22.7 parts of maltodextrin, 7.3-12.1 parts of chitosan, 15.1-30.2 parts of trehalose, 3.8-7.5 parts of glycerinum and 1.1-2.3 parts of ascorbic acid. The molecular weight of the chitosan is 80kDa, and the weight part ratio of the bifidobacterium lactis to the lactobacillus plantarum is 2: 1. In the invention, an inner core is formed through a eutectic structure of bifidobacterium lactis, lactobacillus plantarum and trehalose to protect the probiotics, so that the problems that the existing probiotics are easily influenced by the external environment in the storage and fermentation processes, the activity is reduced, and the fermentation effect of dairy products is poor are solved.

The invention discloses a feed for improving the flavor of broiler chickens and a preparation method of the feed, and belongs to the technical field of poultry feeds and feed preparation. Comprising the following components in parts by weight: 55 to 65 parts of basic ration, 35 to 40 parts of sea buckthorn pomace and bean dreg combined ferment, 3 to 5 parts of aquatic product leftover enzymatic hydrolysate, 3.5 to 4.0 parts of trehalose, 1.0 to 1.5 parts of ground cinnamon, 1.0 to 1.5 parts of xylooligosaccharide, 0.8 to 1.0 part of choline, 0.0001 to 0.0002 part of organic selenium and 0.01 to 0.02 part of folic acid. The mass ratio of the choline to the aquatic product leftover enzymatic hydrolysate is 1: (3.0-5.6); the mass ratio of the organic selenium to the sea buckthorn pomace and bean dreg combined fermentation product is 1: (58-100); the sea buckthorn pomace and bean dreg combined fermentation product is prepared by respectively pretreating sea buckthorn pomace and bean dregs and then carrying out aerobic, facultative and anaerobic continuous three-stage fermentation. The technical limitations that flavor substances of traditional feed are easy to inactivate and the utilization rate of agricultural by-products is low are broken through, and the breakthrough of flavor quality upgrading, efficient resource conversion and breeding benefit optimization is achieved.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention belongs to the technical field of colloidal gold immunochromatography test strips, and particularly relates to an enhanced colloidal gold immunochromatography test strip pretreatment solution and a preparation method thereof.The pretreatment solution comprises a sample pad pretreatment solution and a conjugate pad pretreatment solution, and the sample pad pretreatment solution comprises trehalose, Tween 20, BSA, a Proclin300 preservative and a borate buffer solution; the conjugate pad pretreatment solution comprises trehalose, Tween 20, BSA, PEG20000, a Proclin300 preservative, polyvinyl alcohol, chitosan and a borate buffer solution, and the enhanced colloidal gold immunochromatography test strip pretreatment solution can enhance the binding force between an antibody on the conjugate pad and colloidal gold while improving the permeability and diffusivity of a sample pad, and maximally maintain the activity of the antibody; and the detection sensitivity and accuracy are always kept at excellent levels, so that the overall performance of a colloidal gold immunochromatography detection product is improved.