3026 results about "Trehalose" patented technology

Yogurt containing lactic acid bacteria and Bifidobacteria, to which one or more kinds of sweet substances selected from a group consisting of palatinose, palatinite, maltose, maltitol, starch syrup, reducing starch syrup and trehalose as well as a refined fish oil containing highly unsaturated fatty acids such as DHA and EPA are added, is packed in an oxygen blocking hermetic package. The yogurt contains highly unsaturated fatty acids and has good flavor and taste, as well as excellent product quality stability, and does not generate a fishy odor during normal storage periods.

The present invention has an object to provide a means for durably exerting an action of enhancing collagen-production, and the object is attained by providing a collagen-production enhancer which contains, as an effective ingredient, α,α-trehalose and/or a saccharide derivative thereof, and by a composition incorporated with the collagen-production enhancer to be used for such purpose.

The present invention relates to a method and composition for the cryopreservation of adipose tissue with the intention to use this tissue in the culturing of stem and/or progenitor cells. The method uses a specific cryoprotection medium to prevent damage of the original tissue during the cryopreservation while still maintaining a high viability of the stem and/or progenitor cells obtained from the cryopreserved adipose tissue. Furthermore the cryoprotection medium of the present invention does not contain any kind of xenogeneic sera, a critical factor since it is the intention of that the cryopreserved tissue is used for obtaining stem and/or progenitor cells that can be used in medicine. The cryoprotection medium is characterized in that it is a solution of physiological water comprising glycerol and sucrose and/or trehalose and optionally serum albumin.

A method is provided for preserving live bacteria by subjecting an aqueous system containing the growing bacteria to drying without special equipment, in the presence of trehalose with or without the addition of divalent cations as stabilizing agents. Further, a dried composition for preservation of aerobic bacteria in a viable state is provided. The dried composition consists essentially of dried viable aerobic bacteria and an appropriate growth medium. The bacteria and growth medium are initially placed in an aqueous solution of 10 mM to 200 mM trehalose and a divalent cation, and dried at room temperature.

The present invention has an object to provide an external dermatological formulation having satisfactory blood flow-promoting effect, antiinflammatory effect, antibacterial effect, moisturizing effect, whitening effect, UV-absorbing effect, UV-scattering effect, antioxidant effect, hair growing effect, hair nourishing effect, emusifying effect, astringent effect, wrinkle-reducing effect, cell-activating effect and/or transdermal absorption-promoting effect with a satisfactory safety and skin feeling; The object is solved by providing an external dermatological formulation comprising a saccharide derivative of α,α-trehalose and one or more members selected from substances having any one of blood flow-promoting effect, antiinflammatory effect, antibacterial effect, moisturizing effect, whitening effect, UV-absorbing effect, UV-scattering effect, antioxidant effect, hair growing efect, hair nourishing effect, emulsifying effect, astringent effect, wrinkle-reducing effect, cell-activating effect and transdermal absorption-promoting effect.

The invention relates to the field of stem cells, discloses a method for preparing exosome freeze-dried powder of stem cells, and particularly relates to a method for preparing exosome freeze-dried powder of human amniotic mesenchymal stem cells. The method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention comprises the following steps: mixing trehalose with exosomes of the human amniotic mesenchymal stem cells as a cryoprotectant; filtering and removing bacteria by virtue of a filter membrane; and firstly carrying out ultralow temperature pre-freezing, and then carrying out low-temperature freezing at a certain vacuum degree. The preparation method disclosed by the invention is simple to operate, safe and effective. An experiment proves that compared with a conventional preparation method of the freeze-dried powder, the morphologies and the activity of the exosomes of the human amniotic mesenchymal stem cells can be well kept by the method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention; and the method is suitable for long-term preservation and application of the exosomes of the human amniotic mesenchymal stem cells.

The invention discloses a complex microbial agent for producing soybean paste in Pixian County, which mainly comprises coculture of saccharomyces cerevisiae, candida utilis, lactobacillus plantarum and salt tolerant tetrads, and skim milk powder, glycerin, maltodextrin, trehalose and L-sodium glutamate, or mainly comprises coculture of saccharomyces cerevisiae, candida utilis, lactobacillus plantarum and salt tolerant tetrads, and porous starch, maltodextrin and polyvinyl pyrrolidone. A method for preparing the complex microbial agent for producing soybean paste in Pixian County comprises the following technological steps of: (1) activating and propagating strains; (2) inoculating; (3) co-culturing; and (4) adding a protective agent and drying. By using the complex microbial agent in the preparation of cooked soybean paste, the complex microbial agent maintains the characteristics of traditional soybean paste in Pixian County. Meanwhile, compared with the traditional technology, the complex microbial agent has the advantages of shortening the production period by 1/3, improving the amino nitrogen content by 3 to 8 times, improving the volatile aroma content by 2 to 4 times, and ensuring the aflatoxin B1 of 0 to 0.5ppm only.

The present invention discloses a kind of frozen tilapia mossambica fish fillets containing freezing protective agent trehalose and its processing method. Said processing method includes the following steps: making the tilapia mossambica fish undergo the processes of cleaning dressing, boning, skinning and filleting treatments, then adding protective agent to treat fish fillets, taking out said fish fillets, draining to remove excess solution and freezing below-18 deg.C so as to obtain the invented frozen tilapia mossambica fish fillets. The described protective agent is an aqueous solution containing trehalose, glycine and sodium chloride.

Conversion of renewable hydrocarbons to transportation fuels is required to reduce carbon emission, limit the use of fossil fuels, and develop renewable energy sources. Sorbitol, xylitol and trehelose are polyalcohols generated from the liquefaction of various sugars and carbohydrates in biomass from algae, corn, sugarcane, switchgrasses, and biological wastes. Mixtures of aqueous polyols and fuel feedstocks are catalyzed over metal catalysts to produce hexanes, pentanes, and lighter hydrocarbons. By managing the catalyst, reaction conditions and sulfur content, the octane value of the product fuel is dramatically increased.

The invention discloses a functional composition with functions of controlling blood sugar and enhancing nutrition, which is prepared from main materials and a common carrier, wherein the main materials consist of functional sugars, composite vitamins, mineral substance, dietary fiber and high sweetener; and the functional sugars comprise L-arabinose and trehalose, the weight content of the L-arabinose is 10 to 80 percent, and the weight content of the trehalose is 10 to 50 percent. The functional composition uses two functional sugars as main sugar-reducing substances and is added with multiple composite vitamins, the mineral substance, the dietary fiber and the high sweetener, so the invention provides the functional composition with convenient use, good mouthfeel, safety, effectiveness and practicability of controlling blood sugar and enhancing nutrition for diabetes patients. The functional composition can well supplement nutrient substances required for the human body when the diabetes patients can feel relieved to enjoy a proper amount of sweet food.

The invention discloses a Process for the purification and separation of trehalose, containing enzymolysis reaction which adds saccharify enzyme in enzyme reaction liquor which is getted from the produced trehalose by enzymic conversion method and inoculating 3-10wt% Saccharomyces cerevisiae in saccharify enzyme enzymolysis liquor, fermenting and culturing in the condition of 25-30deg C and pH 5. 5-7. 5, then getting trehalose pure product after centrifuging, hyperfiltration, ion-exchange, condensation, crystallization and desiccation. Comparing with prevenient activated charcoal column chromatography and organolite process, this invention using two-stage remove mixed sugar of enzymolysis liquor, detecting the getted trehalose pure product by HPLC, its purity is as high as 98. 0%. at the same time, this invention has the advantage of high detaching and purifying efficiency, few lose of trehalose, low cost, simple technology and so on, bringing active impel on predigesting productive technology, improving production and reducing production costs of trehalose's produce by enzyme method.

The invention discloses a fresh shrimp freezing and antistaling agent as well as a fresh shrimp freezing and antistaling method. The antistaling agent is a mixture containing tea polyphenol, chitosan, antibacterial peptide, nisin, glucose oxidase, sodium chloride, lactic acid and trehalose. The antistaling agent is used for freezing and fresh-keeping of fresh shrimps; the fresh shrimp freezing and antistaling agent has natural components, and can be used for better protecting freshness of aquatic products, maintaining original appearance and state, reducing loss of proteins and other nutrients, inhibiting generation of bacteria, and substantially prolonging shelf-life and storage life.

A nutritional composition for controlling blood sugar level comprising a protein, a lipid and a carbohydrate, wherein energy percentages supplied by the protein, lipid and carbohydrate are 10 to 25%, 20 to 35% and 40 to 60%, respectively; and oleic acid in the lipid energy percentage is 60 to 90% and palatinose and/or trehalulose in the carbohydrate energy percentage is 60 to 100%. The composition is useful as an oral or tube feeding nutrient for nutritional management or blood sugar level control of patients suffering from diabetes and glucose intolerance, or for obesity prevention, a therapeutic diet, a diet for diabetic patients at home, an obesity preventive diet or a food with health claims.

The invention relates to gum-protecting whitening toothpaste. The gum-protecting whitening toothpaste is prepared from the following components: sorbitol, xylitol, sodium phytate, brilliant blue pigment, sodium lauroyl sarcosine, ethylene diamine tetraacetic acid (EDTA) disodium, plant chitosan, trehalose, sodium saccharin, sodium pyrophosphate, glycerol, sodium hyaluronate, xanthan gum, hydroxyethyl cellulose, polyvinyl pyrrolidone (PVP) cross-linking agent, shell powder, silicon dioxide (friction type), silicon dioxide (thickening type), zinc citrate, lysozyme, pawpaw extract, vitamin E, peppermint essence, silica (whitening particles) and pure water. The components coordinate with one another, so that the effects of protecting gum and whitening teeth are achieved.

The invention relates to a composition used as a vaccine freeze-drying protective agent, comprising the components with initial concentration in the freeze-drying stock solution as follows: 30-100g/l sucrose, 10-30g/l trehalose, 10-50g/l dextran, 6-12g/l sodium glutamate, 3-9g/l urea and 0.5-2g/l arginine, and no gelatin. The invention further provides a method for preparing freeze-drying vaccines by using the freeze-drying protective agent and application of the freeze-drying protective agent for improving the stability of freeze-drying vaccines.

The invention discloses a mask solution. The mask solution contains a moisturizer, wherein the moisturizer is prepared from sodium hyaluronate, trehalose, sodium polyglutamate, betaine, tremella polysaccharide and glyceryl polymethacrylate. The mask solution disclosed by the invention is capable of durably and deeply moisturizing the skin, is good in mildness, is also suitable for skin sensitive persons, is natural and non-toxic, is mild and non-irritant, cannot damage the skin after long-term use, has an obvious effect in improving the skin, and is beneficial for enabling the skin to restore elasticity and be glossy.

The invention discloses a method of using waste bear yeast to simultaneously prepare zymosan, trehalose and yeast extract. The method comprises steps of debittering and impurities removing of the waste bear yeast, autolysis, enzymatic hydrolysis and inactivation of the waste bear yeast, separation and drying of the zymosan, membrane separation, concentration, crystallization and drying of the trehalose, concentration and drying of the yeast extract, etc. The method has the characteristics of high raw material utilization rate, good quality, low cost and easy industrialization, realizes that three products of the zymosan, the trehalose and the yeast extract are simultaneously produced from the same industrial waste, the waste bear yeast, improves the utilization rate of industrial wastes and achieves the target of maximal waste resource recovery.

The invention relates to a recombinant human basic fibroblast growth factor gelling agent and the process for preparation, which comprises recombinant human basic fibroblast growth factor (rh-bFGF) stock solution 0.1-100% (W/W), medicinal auxiliary material 0-99.9% (W/W), the stock solution comprises effective treatment dose of recombinant human basic fibroblast growth factor (rh-bFGF) 0.01-1% (ug/g), NaCl 0.01-20% (W/W), phosphates cushioning liquid 2m Mol - 200m Mol, and right amount of water for injection, the medicinal auxiliary materials can be selected from: carbomer 940NF, sodium hyaluronate, trehalose, mannitol, dextran 40 and sodium heparin.

The invention discloses a mesenchymal stem cell frozen stock solution which does not contain animal proteins and DMSO (dimethyl sulfoxide). The frozen stock solution comprises trehaloses, a carbohydrate and electrolyte injection, ATP (adenosine triphosphate) and human serum albumins. The frozen stock solution has the advantages of high recovery rate (90%), no influence on characteristics of stem cells, unchanged cell proliferation, unchanged cell differentiation, easy use, good stability and the like. The invention also discloses a preparation method of the frozen stock solution.

A lubricant for preventing and treating the vaginal xerosis, pruritus vulvae and leukoplakia vulvae is prepared from polymycose, hyaluronic acid and medicinal auxiliaries.

The invention relates to the field of hypothermal medicine of biotechnology, in particular to a cryoprotectant free of DMSO (dimethyl sulfoxide). The cryoprotectant comprises 200 mmol/L of trehalose, 0.3 g/mL of PVP (polyvinylpyrrolidone) 40, 200 mu g/L of cholesterol and 200 mu g/L of lecithin. The cryoprotectant is injected into a newly obtained ovary; all macroscopic follicles on the surface of the ovary are punctured and sucked, so that content in the follicles is removed; then the ovary is put in a freezing bag to be cooled. The cryoprotectant doesn't contain DMSO, does not have cytotoxicity and is easy to remove, simple to prepare and good in cryoprotective effect, raw materials are easy to obtained, the rate of normal primordial follicles is high, and the number of dead cells is small; an ovary cryopreservation method is simple and easy to operate and good in cryoprotective effect; the histological structure can be better conserved after the whole ovary of sheep is frozen.

An ophthalmic pharmaceutical composition comprising trehalose as an effective ingredient and a pharmaceutically-acceptable carrier. The pharmaceutical composition is a safe, long-term continuously-administrable, therapeutic and/or prophylactic agent for the ophthalmologic clinical symptoms and signs in Sjögren syndrome.

The invention discloses a novel application of malt oligosaccharide based mycose synthetase and malt oligosaccharide based mycose hydrolase in mycose production. The malt oligosaccharide based mycose synthetase has an amino acid sequence as shown in SEQ ID NO:1; and the malt oligosaccharide based mycose hydrolase has the amino acid sequence as shown in SEQ ID NO:3. The malt oligosaccharide based mycose synthetase and malt oligosaccharide based mycose hydrolase disclosed in the invention also have higher most-suitable reaction temperature and thermal stabilities, and lower most-suitable pH values, so that contamination risks are lowered, production stability is improved, the mycose production efficiency by acting reducing glucidtemns through the malt oligosaccharide based mycose synthetase and malt oligosaccharide based mycose hydrolase and pullulanase in a combined manner is improved and the mycose cost is lowered, therefore, a solid foundation is laid up for the extensive application of the mycose in medicines, foods and cosmetics.

The invention relates to a soluble micro-pin patch and a preparation method thereof. The pin point of the micro-pin patch is prepared from, by weight, 7-14 parts of hyaluronic acid or salts thereof, 0.05-0.2 part of functional active ingredients, and 6-11 parts of shaped materials; the hyaluronic acid or salts thereof are mixtures of low molecular weight hyaluronic acid or salts thereof and high molecular weight hyaluronic acid or salts thereof, wherein the molecular weight of the small molecular weight hyaluronic acid or salts thereof is less than 10 kDa, the molecular weight of the high molecular weight hyaluronic acid or salts thereof is not less than 1800 kDa; the functional active ingredients are selected from oligo-peptide-1, oligopeptide-3, hydrolyzed collagen, and at least one of the hyaluronic acid or salts thereof; the shaped materials are selected from polyvinyl alcohol and derivatives thereof, polyvinyl pyrrolidone and derivatives thereof, and at least one of lactose, sorbitol, dextran, trehalose and sucrose. The soluble micro-pin patch has the properties of good rigidity and strength.