655 results about "Biopharmaceutical" patented technology

The invention relates to the technical field of biological pharmacy, and discloses a probiotic microcapsule with a function of improving intestinal microecology, which comprises the following components in percentage by weight: lactobacillus rhamnosus: each gram of formula contains 1010 CFU of lactobacillus rhamnosus; lactobacillus acidophilus: each gram of the formula contains 109 CFU; clostridium butyricum: each gram of the formula contains 108 CFU; 5%-8% of a nano sodium alginate-chitosan compound; 3%-5% of peptide nanoparticles; 2%-4% of a natural polymer intelligent response film; 3%-6% of arabinoxylan oligosaccharide; 4%-7% of resistant dextran; 2%-4% of lentinan; and 0.1%-0.3% of resveratrol. Through a double-layer or three-layer embedding technology of the nano sodium alginate-chitosan compound and the intelligent response polymer film, the tolerance of the probiotics in gastric acid and cholate environments is remarkably enhanced, and targeted release of the probiotics in small intestines and colon is ensured.

The invention discloses a site for stably expressing protein in a CHO cell gene NW023276806.1 and application of the site, and belongs to the technical field of biological genes. The site belongs to a fixed position in a CHO cell genome, different protein genes are introduced based on a micro-homologous end connection mechanism through a CRISPR / Cas9 tool, and stable expression is carried out. By adopting a site-specific integration method, a target gene is integrated to a stable expression area in a site-specific manner, repeated high-expression monoclonal screening is effectively avoided, and an MMEJ mechanism is introduced to integrate a donor fragment, so that the research and development time for constructing a stable expression cell strain in biological pharmacy can be effectively shortened, and the cost is reduced.

The present invention belongs to the field of biopharmaceutical technology, and specifically relates to a method for preparing GLP-1 or its analogs. The present invention provides a leader peptide, and a fusion protein comprising the leader peptide. The leader peptide or fusion protein is used to prepare a semaglutide precursor polypeptide, and the results show that the yield of the prokaryotic expression fusion protein can reach 26g / L, and the purity of the precursor polypeptide obtained after enzyme cleavage is higher than 95%. The precursor polypeptide is further side-chain modified and connected with a dipeptide to efficiently prepare semaglutide.

The invention belongs to the field of biological pharmacy and biotransformation, and particularly relates to a tagatose-4-epimerase mutant and application thereof. The tagatose-4-epimerase mutant disclosed by the invention is obtained by carrying out single mutation or combined mutation on an amino acid residue at a specific position of an amino acid sequence as shown in SEQ ID NO.1. Compared with wild type tagatose-4-epimerase, the mutant has the advantage that the catalytic activity is improved when the D-tagatose is prepared through conversion. The obtained tagatose-4-epimerase mutant has the advantages of being mild in reaction condition, green, environmentally friendly, single in product and easy to separate and purify, and the production cost is greatly reduced.

The invention provides an anti-hair loss composition containing cacumen biotae as well as a preparation method and application of the anti-hair loss composition, and belongs to the technical field of biological medicines. The anti-hair loss composition containing cacumen biotae comprises a cacumen biotae extract, a ginseng root extract, an eclipta prostrata extract, a polygonum multiflorum extract, a tea seed extract, a gleditsia sinensis extract, a mulberry bark extract, a cortex dictamni extract, a cnidium monnieri seed extract and a mint extract. All the components have a synergistic effect, and the shampoo has the effects of improving the greasy environment of scalp, preventing grease from blocking hair follicles, reducing the damage of inflammation to the hair follicles, inhibiting the activity of 5 alpha-reductase, improving the color and toughness of hair and the like, relieves alopecia seborrhoeica and achieves an anti-alopecia effect.

The invention provides a biopharmacy downstream process optimization method and system based on machine learning. The method comprises the following steps: acquiring process parameters, process parameters and key quality attributes from a historical production process; preprocessing the technological parameters, the process parameters and the key quality attributes, performing feature construction, and selecting feature data; training a plurality of pre-acquired machine learning models based on the feature data, identifying key process parameters, evaluating the trained machine learning models according to a preset dimension, and selecting at least one optimal machine learning model according to an evaluation result; and performing parameter combination based on the key process parameters and a preset constraint range, inputting the combined parameters into at least one optimal machine learning model, and selecting a key process parameter combination meeting a preset quality standard according to an output result. According to the method, the problem that the key quality attributes are difficult to accurately predict and optimize in the existing biopharmacy downstream process is solved.

The invention provides a composition capable of penetrating an intestinal barrier by oral administration. The composition contains a carrier or an auxiliary material of a targeted Microflod cell Dectin-1 receptor, and one or / and a plurality of medicinal components / components, wherein the carrier or the auxiliary material of the target Microflood cell Dectin-1 receptor is beta-glucan, and the carrier or the auxiliary material of the target Microflood cell Dectin-1 receptor is beta-glucan. The effective components / components are traditional Chinese medicine effective components / components, or / and probiotics, or / and chemical medicine components, or / and biological medicines. The invention relates to a composition capable of realizing oral administration and penetrating through an intestinal barrier, which is based on the discovery of the inventor that the composition reaches colon through gastrointestinal tracts, targets colon Microflood cells, is taken by the Microflood cells, penetrates through the intestinal barrier and enters immune tissue Colonic Patches of a colon submucosa. Based on the research, the composition disclosed by the invention can be used for treating various diseases, such as ulcerative colitis, Crohn's disease, colon cancer, rheumatoid arthritis or bacterial pneumonia after being orally taken and absorbed by rectum.

The invention provides an AS01 derivative adjuvant, a preparation method thereof and application of the AS01 derivative adjuvant in preparation of vaccines or anti-tumor drugs, and belongs to the technical field of biological drugs or products. The AS01 derivative adjuvant is prepared from the following components in parts by mass: 0.5 to 1.0 part of cholesterol, 2.0 to 4.0 parts of dioleoyl lecithin and at least one of the following components in parts by mass: 0.1 to 0.2 part of monophosphoryl lipid A, 0.1 to 0.2 part of QS-21, 0.36 to 0.72 part of 3M-052, 0.4 to 0.8 part of muramyl dipeptide and 0.2 to 0.4 part of oligodeoxynucleotide. According to the AS01 derived adjuvant, a novel synergistic adjuvant system is developed by integrating various immunomodulators, so that the immunogenicity of a vaccine is enhanced. The HPV vaccine is prepared on the basis of the AS01 derived adjuvant, so that the immunogenicity can be enhanced, and good anti-tumor activity can be achieved. Therefore, the invention provides a new strategy for immunotherapy of HPV-related tumors.

A series of viscosity-reducing and stabilizing formulations for high concentration protein formulations, which are meticulously tailored to significantly decrease the viscosity and increase the stability of high concentration biopharmaceuticals. The viscosity-reducing and stabilizing formulations can be used in high concentration biopharmaceutical products and can bring considerable potential for enhancing the stability during preparation and storage and elevating the feasibility of high concentration biopharmaceutical products.

The invention discloses a serum-free full-suspension MDCK (Madin-Darby Canine Kidney) cell and application thereof in preparation of influenza vaccines, and belongs to the field of virology and biological medicines. The serum-free full-suspension MDCK cell is preserved in the China Center for Type Culture Collection, is classified and named as a canine kidney cell MDCK-JHS, and has a preservation number of CCTCC NO: C2024373. The invention further provides a method for culturing the influenza virus by using the cell line, high-titer amplification of the influenza virus can be realized, and the method is suitable for industrial production.

The invention belongs to the technical field of biological medicine manufacturing, and particularly relates to multifunctional integrated equipment for processing traditional Chinese medicine decoction pieces, which comprises a bottom plate and the like, a supporting seat is fixedly connected to the bottom plate, the bottom plate and the supporting seat are load-bearing carriers of the equipment, the processing tank is mounted at the top of the supporting seat, a traditional Chinese medicine powder discharging port is formed in the top of the processing tank, a traditional Chinese medicine dry product scrap recycling port is formed in the bottom of the processing tank, and the processing tank is a container for processing traditional Chinese medicine decoction pieces. Through the design of the high-pressure air pump and the flow guide pipe, the discharging pipe is communicated into the flow guide pipe, airflow forms high-speed opposite impact in the processing tank, mutual impact of materials is effectively promoted, the smashing efficiency is improved, the spiral conveying roller is driven by the servo motor, dried traditional Chinese medicine materials regularly enter the flow guide pipe, the flow guide pipe is prevented from being blocked, and the processing efficiency is improved. And the crushing effect is improved by spraying a proper amount of materials every time.

The invention provides a probiotic composition for promoting ethanol metabolism as well as a probiotic agent and application thereof, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: compounding lactobacillus mucus A21252, plant lactobacillus A21352, casei paracasei A21151 and lactobacillus reuteri A21325 to obtain a probiotic composition, and matching the probiotic composition with auxiliary materials (resistant dextrin, xylooligosaccharide, erythritol, L-arabinose, lemon fruit powder and corn oligopeptide powder) to obtain the probiotic agent. The probiotic agent not only can quickly improve the hangover alleviating speed, has the effects of alleviating hangover, protecting liver and eliminating gastrointestinal dysfunction caused by alcoholism, but also can reduce discomfort caused by excessive drinking. The probiotic agent is simple and convenient to process, low in cost and wide in market prospect.

The invention relates to the field of biological medicine preparation, and provides a semi-synthesis preparation method of semeglutide and a preparation method of a semeglutide side chain, the semi-synthesis preparation method comprises the following steps: 1, in the presence of a first base catalyst, mixing a compound SMG-1 solution with a compound III solution, reacting to obtain a compound IV, and filtering to obtain a compound III; wherein the addition amount of the first base catalyst is such that the pH value of the SMG-1 solution is 11.2-11.6; 2, in the presence of a coupling agent and a second base catalyst, a compound IV solution and a compound SMG-3 solution are mixed and coupled to obtain a compound V, and the pH value in the step 2 is 9.5-10.0; and 3, carrying out deprotection on the compound V by using a deprotection agent to finally obtain the semeglutide. The method provided by the invention has the advantages of few reaction steps, reduced cost, high semeglutide purity and high yield, and is suitable for large-scale industrial production.

The invention belongs to the technical field of biological medicine, and particularly relates to a novel deuterated JAK2 inhibitor or pharmaceutically acceptable salt thereof. Compared with the prior art, the compound or the pharmaceutically acceptable salt thereof provided by the invention has better JAK2 inhibition activity, and the JAK2 inhibition target selectivity of the compound or the pharmaceutically acceptable salt thereof is obviously superior to that of the existing compound, so that the compound or the pharmaceutically acceptable salt thereof has better druggability.

The invention belongs to the technical field of biological pharmacy, and relates to a method for preparing tremella aurantialba polyphenol by utilizing tremella aurantialba TA58.21 fungus chassis cell fermentation, which comprises the following steps: adding a cooked soybean meal culture medium and a tremella aurantialba seed solution into a fermentation tank, introducing sterile oxygen, controlling the stirring rotating speed of the fermentation tank and the culture temperature to be 25-30 DEG C, continuously culturing for 5-8 days, and when the fermentation liquor becomes red, stopping the fermentation; when a large amount of foam is generated, closing an exhaust valve, keeping the tank pressure at 0.1 MPa, increasing the fermentation temperature to 50 DEG C, continuing to culture for 24 hours, increasing the stirring rotating speed of the fermentation tank, heating the fermentation liquor to 80-90 DEG C, keeping the temperature for 4-8 hours, stopping stirring, continuing to heat to 120 DEG C, and keeping the temperature for 30 minutes; centrifuging the fermentation liquor to remove precipitates and mycelia; collecting supernate, and pre-treating macromolecular substances and thallus fragments; collecting permeate liquid, and intercepting through a nanofiltration membrane to obtain a concentrated solution; and carrying out spray drying or freeze drying treatment on the concentrated solution to obtain a high-purity product. According to the invention, the tremella aurantialba polyphenol product with good water solubility, heat resistance, acid resistance and molecular weight of less than 10000 Da can be obtained.

The utility model discloses biopharmaceutical capsule subpackaging equipment which comprises a workbench, a stand column is arranged at the upper end of the workbench, a material box is arranged at the upper end of the stand column, a first supporting rod is arranged on the left end face of the stand column, and a second supporting rod is arranged on the right end face of the stand column. A first hopper and a second hopper are arranged at one end of the first supporting rod and one end of the second supporting rod respectively, a left outlet and a right outlet are formed in the left end face and the right end face of the material box respectively, parallel blocks are arranged on the front inner wall and the rear inner wall of the material box, supporting blocks are arranged on the front end faces of the parallel blocks, and a grain counting plate is arranged at the upper ends of the supporting blocks. The tablet counting plate is divided into a left area and a right area, the left area and the right area are respectively provided with a plurality of capsule grooves, a left baffle is arranged at the left end of the tablet counting plate, a right baffle is arranged at the right end of the tablet counting plate, plate drawing grooves are formed in the outer side surfaces of the left baffle and the right baffle, and bottom drawing plates are arranged in the plate drawing grooves. And a pulling plate is arranged at one end of the outer side of the bottom pulling plate.

The utility model discloses a biological medicine refrigeration device which comprises a cup body and a cup cover detachably connected with the cup body, an inner / outer double-thread structure is arranged on the cup cover, the outer thread structure is connected with the cup body, the inner thread structure is detachably connected with a cold / heat conduction cylinder, refrigeration equipment is arranged in the cup cover, and the refrigeration equipment is connected with the cup body. A buffer structure is arranged on the periphery of the refrigeration equipment, the refrigeration equipment is electrically connected with an electric control system located on the cup cover, the cup cover further comprises an external heat dissipation mechanism connected in a detachable mode, and the electric control system is electrically connected with a sensor through a connecting wire. And the sensor extends into the medicine storage space in the cup body. The probe of the temperature sensor extends into the medicine storage space in the cup body, the displayed temperature is more appropriate and accurate, the cup is convenient to carry, cold and heat sources are prevented from being in direct contact with biological medicines, and the biological medicines are prevented from losing activity to affect the medicine effect due to too low and too high storage temperature.

The invention belongs to the field of biological medicines, and discloses an application of a protein polymer in preparation of a medicine for treating Alzheimer's disease, a production process of the protein polymer comprises the following steps: S1) using ultraviolet irradiation to stimulate mesenchymal stem cells and culturing; and S2) splitting the mesenchymal stem cells, separating and purifying to obtain the protein polymer. According to the present invention, the mesenchymal stem cells are stimulated to express the protein with the nerve repair function through the ultraviolet irradiation stress mode, and the protein polymer is obtained through further separation and purification, such that the protein polymer can be adapted to diversified application modes, can improve the abnormal conditions of the Alzheimer's disease related biomarkers, and can provide the significant treatment effect on the Alzheimer's disease.

Use of PGLYRP2 gene and a related drug thereof. The present invention relates to the field of biopharmaceuticals, and in particular, to use of the PGLYRP2 gene and the related drug thereof. The present invention aims to solve the problem that existing drugs can only inhibit the replication of hepatitis B virus and cannot eliminate viral cccDNA. The PGLYRP2 gene is used for preparing a drug for treating a hepatitis B virus infection. The PGLYRP2 gene is used for preparing a drug for eliminating hepatitis B virus cccDNA. Also provided is a gene therapy vector for eliminating hepatitis B virus. The gene therapy vector comprises a promoter, an intron, an enhancer, and a PGLYRP2 gene encoding region. The HBV virus inhibition function and the cccDNA-eliminating effect of the PGLYRP2 protein are defined, so that the elimination of HBV in liver cells and a mouse liver can be significantly promoted.

The invention belongs to the technical field of biological medicines, and particularly relates to a combined preparation of chlorbuconazole or pharmaceutically acceptable salt thereof and a proline hydroxylase inhibitor medicine. The combined use of the chlorbuconazole and the proline hydroxylase inhibitor drug can activate the expression of the downstream target gene of the HIF-2alpha, and a synergistic effect is shown. In a cisplatin-induced renal anemia model, the chlorprazole acid can enhance the effect of the proline hydroxylase inhibitor on treating renal anemia.

The invention belongs to the field of biological medicines, and discloses a stress-induced mesenchymal stem cell extract and a preparation method and application thereof.The preparation method comprises the steps that S1, mesenchymal stem cells are cultured under the high-temperature condition, swelling and cracking are conducted on the mesenchymal stem cells, or supernate obtained after culture of the mesenchymal stem cells is taken and filtered, and filtrate is obtained; the temperature of the high temperature ranges from 37.0 DEG C to 42.0 DEG C; s2, purifying the mesenchymal stem cell extract by adopting a molecular sieve exclusion chromatography method or a reverse chromatography method. Specific high-temperature stimulation is adopted to induce the mesenchymal stem cells, a purification method is combined, protein expression is promoted, the purity of the mesenchymal stem cell extract is improved, the prepared and obtained purified Alibitumen is used in a cell oxidative damage model, and the cell oxidative damage rate is increased. It is found that all the Aribitua derived from high-temperature stress culture has high oxidative damage repairing capacity.

This invention belongs to the field of biopharmaceuticals and provides a keratin YK93-6, its encoding nucleic acid molecule, an expression vector containing the nucleic acid molecule, a host cell containing the expression vector or whose genome integrates the nucleic acid molecule, as well as a preparation method and a pharmaceutical composition thereof. It also provides the application of the above-mentioned keratin YK93-6 and other products in the preparation of drugs for treating benign prostatic hyperplasia, lymphoma, melanoma, analgesia, lactation, breast cancer, lung cancer, uterine fibroids, coagulation, etc.

The invention discloses a dust-free environment purification method for biological pharmacy. Comprising the following steps: constructing three layers of metal filter screens, realizing particle interception through a conical micro-column array, an Al2O3 / TiO2 composite ceramic layer and a diamond-like carbon film, fixing lysozyme by adopting a crystal face regulated ZIF-8 crystal, and realizing dual inactivation of microorganisms by combining dielectric barrier discharge of a spiral plasma channel. The electrostatic adsorption HEPA filter paper adopts a BaTiO3-graphene quantum dot composite electret substrate, and a TiO2 / graphene heterojunction catalytic layer is decomposed by a Pt-Co bimetallic nanocluster and a photocatalysis-plasma-ozone-thermocatalysis quadruple oxidation system; and 0.1 mu m organic aerosol triggers graded reverse pulse cleaning based on differential pressure integration and a particle concentration threshold value, and the honeycomb flow guide structure drives a shutter and a gradient density sintering metal flow guide plate through piezoelectric ceramics to maintain laminar flow uniformity. According to the scheme, the limitation of a traditional technology is broken through, the purification efficiency of 0.1 micron particles is improved, and a high-stability solution is provided for a biopharmaceutical clean room.

The invention relates to the field of biological medicine manufacturing, in particular to a preparation method and application of nanoparticles for destroying hepatoma cell ferritin, and provides ultrasonic response type nanoparticles (NPs) composed of polylactic acid-glycolic acid (PLGA) modified by ferritin homing peptide (HKN15) and acoustic droplet vaporization (ADV) material perfluorohexane (PFH). Through the positive feedback effect of overexpression of the HKN15 and the tumor cell ferritin, the NPs (HKN15-coated PLGA-PFH) specifically gathers around the ferritin in the HCC cell. Tumor cells can be directly killed by a cavitation effect generated by ADV through ultrasonic irradiation of a tumor area; more importantly, along with collapse of ferritin, the concentration of iron in cells is increased, so that inevitable ferroptosis is caused. The scheme can destroy hepatoma cell ferritin, increase the concentration of free iron in the hepatoma cell ferritin and induce a ferroptosis effect to achieve the purpose of tumor treatment.

The invention belongs to the field of biological medicines, and discloses an application of a protein polymer in preparation of a medicine for treating spinal cerebellar ataxia disease, a production process of the protein polymer comprises the following steps: S1) using ultraviolet irradiation to stimulate mesenchymal stem cells and culturing; and S2) splitting the mesenchymal stem cells, separating and purifying to obtain the protein polymer. An ultraviolet irradiation stress mode is adopted, mesenchymal stem cells are stimulated to express protein with a neural repair function, the protein polymer is obtained through further separation and purification, the cell gene expression pathway is regulated, the cell stress state is relieved, the cells are promoted to recover normal forms and functions, meanwhile, the preparation mode is relatively simple, and the preparation method is suitable for large-scale production. And quality and yield monitoring is facilitated, and the method can be adapted to diversified application modes.

The invention belongs to the field of biological pharmacy, and relates to an alpha-amylase inhibitory peptide and application thereof. Specifically, the provided unreported peptide fragment LDKFLWT can effectively inhibit the activity of alpha-amylase, and animal experiments prove that the newly designed polypeptide has the effects of reducing blood sugar, reducing blood fat, improving insulin resistance and the like through polypeptide intervention; compared with a traditional hypoglycemic drug, the alpha-amylase inhibitory peptide disclosed by the invention has remarkable safety.

The utility model discloses an adjustable glass reaction kettle, which belongs to the technical field of biological pharmacy and comprises a base, a glass reaction kettle body is arranged above the base, a kettle cover is arranged above the glass reaction kettle body, a convenient switch component is arranged at one end of the kettle cover, and a support is arranged above the convenient switch component. A frequency converter is arranged on one side of the support, a collecting assembly is arranged in the middle of the base, a stirring assembly is arranged in the glass reaction kettle body, a condenser is arranged at one end of the upper portion of the kettle cover, and a constant-pressure funnel is arranged at the other end of the upper portion of the kettle cover. According to the structure, the lifting plate can be used for adding materials or cleaning and maintaining the interior, when the structure is used, after the kettle cover is opened, the lifting plate can be limited, the stability after the kettle cover is opened is improved, and by arranging the collecting assembly, the discharged materials can be conveniently collected and treated.

The invention relates to the field of biological medicine preparation, and particularly provides liraglutide precursor peptide tandem recombinant fusion protein, polynucleotide, recombinant expression plasmid, engineered host cell and a method for preparing target polypeptide. The recombinant fusion protein is fusion peptide-target polypeptide-(linker peptide-target polypeptide) n from the N terminal to the C terminal, n is a positive integer from 4 to 6, the fusion peptide is SEQ ID NO.1, the target polypeptide is liraglutide precursor peptide, the linker peptide comprises a spacer peptide and a protease restriction enzyme cutting site, the spacer peptide is SEQ ID NO.2, the isoelectric point of the recombinant fusion protein is 4.6-4.7, and the average hydrophilic value is-0.780--0.765. The proportion of the target polypeptide in the recombinant fusion protein is high, use of solvents under extreme conditions is avoided in the production process, the enzyme digestion efficiency is high, and the product purity and yield are high.

The invention relates to the field of computational biology and bioinformatics, in particular to an RNA (Ribonucleic Acid) sequence screening and promoter function predicting and screening method based on machine learning, which comprises the following steps of: firstly, adopting a novel sequence algorithm based on a sliding window and a position weight matrix, and combining technologies such as k-mer frequency analysis and conservative motif recognition; candidate RNA sequences are efficiently identified from genomic sequences. Then, an integrated prediction model fusing deep learning and traditional machine learning is established, and multi-dimensional information such as sequence features, structural features, functional features and evolution features is extracted; and carrying out batch prediction on the candidate RNA sequences, outputting a function intensity score and providing credibility evaluation. And finally, screening an RNA sequence with an optimal prediction result based on a multi-objective optimization strategy, and designing an experimental verification scheme. According to the method, the prediction accuracy and the screening efficiency are improved, the experiment cost can be remarkably reduced, and an efficient promoter screening tool is provided for the fields of synthetic biology, gene therapy, biological pharmacy and the like.

This invention belongs to the field of biopharmaceuticals and provides a keratin YK93-8, its encoding nucleic acid molecule, an expression vector containing the nucleic acid molecule, a host cell containing the expression vector or whose genome integrates the nucleic acid molecule, as well as a preparation method and a pharmaceutical composition thereof. It also provides the application of the above-mentioned keratin YK93-8 and other products in the preparation of drugs for treating benign prostatic hyperplasia, lymphoma, melanoma, breast cancer, lung cancer, analgesia, lactation, uterine fibroids, coagulation, etc.