The invention discloses a
sound wave-assisted fishbone microfluidic
separation system for ultrahigh-flux separation of exosomes and a preparation method and application thereof, a
core component of the
system comprises a
microfluidic chip with an upper and lower layer structure, a fishbone-shaped channel and three access ports are arranged in the
chip, and a pair of piezoelectric transducers is arranged outside the
chip. The nano-porous AAO film is placed between the upper layer and the lower layer of the
chip, and the chip is tightly sealed. The design of the fishbone-shaped
microfluidic channel aims at strengthening the
mixing effect of particles in a solution and the contact between the particles and a membrane interface. The nano-porous AAO film is induced to oscillate through sound
waves generated by the piezoelectric
transducer, and the mechanism effectively relieves the problem that the
exosome is blocked in the nano-pores. Due to the
size selectivity of the
nano porous membrane, small molecular substances such as
protein and
nucleic acid are allowed to pass through smoothly, and the
exosome with larger volume is intercepted in the micro-
fluidic channel, so that the efficient enrichment of the
exosome is realized. By utilizing the device, the exosome can be collected and eluted in situ within a short time.