The invention discloses a micro-fluidic
chip for capturing exosomes as well as a preparation method and a use method of the micro-fluidic
chip, and belongs to the technical field of biomedical detection and micro-nano
processing. The
chip adopts a multi-layer
composite structure formed by sequentially stacking an upper clamp layer, an upper micro-channel layer, a functional nano
composite film layer, a lower micro-channel layer and a lower clamp layer from top to bottom; the inner surface of the upper micro-channel layer is provided with a capture channel containing a periodic herringbone
microstructure, and the inner surface of the lower micro-channel layer is provided with a blank channel; the functional nano-
composite film layer is clamped between the upper layer and the lower layer and is made of a
polycarbonate track
etching nano-
porous film of which the surface is covalently modified with TIM4
protein. The principle of tangential flow
filtration and immunoaffinity capture is combined, efficient and high-specificity capture of the
exosome is achieved, and mild and reversible release of the
exosome can be achieved through EDTA
chelation. The device has the advantages of high capture efficiency, simplicity and convenience in operation, low cost and the like, and is suitable for separation and purification of exosomes in the field of
biomedicine.