Rapid detection and quantitation of pathogen-specific biomarkers using nanoporous dual- or multi-layer silica films

a biomarker and nanoporous technology, applied in the field of molecular biology and medicine, can solve the problems of increasing the difficulty of identifying tb in the highly vulnerable human-immunodeficiency virus population, and the difficulty of tb detection in pediatric patients, so as to facilitate the fractionation and digestion of samples on the chip, and facilitate the detection and quantification of such biomarkers. the effect of high detection accuracy
US20150260715A1Inactive Publication Date: 2015-09-17THE METHODIST HOSPITAL
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
THE METHODIST HOSPITAL
Publication Date
2015-09-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

Improved methods for detecting active tuberculosis are disclosed. A method comprises enriching at least one M. tuberculosis-specific biomolecule from a sample by contacting the sample with a nanoporous film; and detecting the presence of the M. tuberculosis-specific biomolecule or fragment(s) thereof. The method may further comprise digesting the enriched M. tuberculosis-specific biomolecule with an enzyme to produce a digestion product comprising at least one fragment of the M. tuberculosis-specific biomolecule. Improved sensitivity and speed achieved.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present application claims priority to PCT Intl. Pat. Appl. No. PCT / US2013 / 072416; filed Nov. 27, 2013 (pending; Atty. Dkt. No. 37182.163), which claims the benefit of U.S. Prov. Pat. Appl. No. 61 / 732,266, filed Nov. 30, 2012 (expired; Atty. Dkt. No. 37182.162), the contents of each of which is specifically incorporated herein in its entirety by express reference thereto.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

[0002] This invention was made with government support under Grant No. W81XWH-11-2-0168 awarded by the United States Department of Defense, and Grant No. U54-CA-151668 awarded by the National Institutes of Health. The government has certain rights in the invention.NAMES OF THE PARTIES TO A JOINT RESEARCH AGREEMENT

[0003] Not Applicable.BACKGROUND OF THE INVENTION

[0004] 1. Field of the Invention

[0005] The present invention relates to the fields of molecular biology and medicine. In particular, the invention prov...

Claims

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