560 results about "Ms analysis" patented technology

An imbalanced radio frequency (RF) field creates a retarding barrier near the exit aperture of a multipole ion guide, in combination with the extracting DC field such that the barrier provides an m/z dependent cut of ion sampling. Contrary to the prior art, the mass dependent sampling provides a well-conditioned ion beam suitable for other mass spectrometric devices. The mass selective sampling is suggested for improving duty cycle of o-TOF MS, for injecting ions into a multi-reflecting TOF MS in a zoom mode, for parallel MS-MS analysis in a trap-TOF MS, as well as for moderate mass filtering in fragmentation cells and ion reactors. With the aid of resonant excitation, the mass selective ion sampling is suggested for mass analysis.

A device and method for capture of magnetic beads in a rotary magnetic bead trap is disclosed. The device allows capture, washing, elution and ejection of beads in an automated system. Analyte is eluted in a small volume in a capillary-scale fluid system compatible with LC-MS/MS analysis.

To provide comprehensive (i.e. rapid and sensitive) MS-MS analysis, the inventor employs a time-nested separation, using two time-of-flight (TOF) mass spectrometers. Parent ions are separated in a slow and long TOF1, operating at low ion energy (1 to l00eV), and fragment ions are mass analyzed in a fast and short TOF2, operating at much higher keV energy. Low energy fragmentation cell between TOF1 and TOF2 is tailored to accelerate fragmentation and dampening steps, mostly by shortening the cell and employing higher gas pressure. Since separation in TOF1 takes milliseconds and mass analysis in TOF2- microseconds, the invention provides comprehensive MS-MS analysis of multiple precursor ions per single ion pulse. Slow separation in TOF1 becomes possible with an introduction of novel TOF1 analyzers. The TOF-TOF could be implemented using a static TOF1, here described on the examples of spiratron, planar and cylindrical multi-pass separators with griddles spatial focusing ion mirrors. Higher performance is expected with the use of novel hybrid TOF 1 analyzers, combining radio frequency (RF) and quadratic DC fields. RF field retains low-energy ions within TOF 1 analyzer, while quadratic DC field improves resolution by compensate for large relative energy spread.

The present invention facilitates improvements in laser ablation of solid samples to be analyzed by an external inductively coupled plasma (ICP) emission spectrometer, ICP/mass-spectrometer (ICP-MS), or flowing afterglow (FAG) mass spectrometer (FAG-MS) for elemental analysis (ICP and ICP-MS) or molecular analysis (FAG-MS). A novel invention mirror-with-hole beam combiner eliminates chromatic aberration in the invention sample view and allows rad-hardening the laser ablation invention for use in a radiation hot cell for analysis of high activity nuclear waste. Many other novel invention rad-hardening attributes facilitate a comprehensive rad-hardened laser ablation system (the world's first). In other embodiments, invention novelties include unusually large homogeneous focused laser spot diameters, unusually long laser objective lens focal length, wide range operationally variable laser path length with built-in re-alignment, operationally variable demagnification ratio and diameter of the focused laser spot, the use of significantly higher powered SMR lasers in a large spot diameter to facilitate high sensitivity bulk analysis of solid samples, a demountable and gravitationally self-sealing stack assembly laser ablation cell, and the world's first auto-samplers (mechanized sample changers) for analytical laser ablation.

The invention discloses a method for measuring sterol in tobaccos. The method comprises the following steps of: (1) deploying sterol standard templates containing internal standard with different concentrations, carrying out GC-MS (Gas Chromatography-Mass Spectrometer) analysis after derivatization, and plotting by utilizing the ratio of the sterol to internal standard peak area and the ratio of the sterol to internal standard concentration to obtain a standard curve; (2) carrying out acid hydrolysis, saponification and direct ultrasonic extraction on samples to be measured, and taking 1 muL for carrying out GC-MS analysis after derivatization; and (3) reading the ratio of the sterol to the internal standard concentration from the standard curve by utilizing the ratio of the sterol to theinternal standard peak area, and then calculating the amount of the sterol. The invention can be used for analyzing cholesterol, ergosterol, campesterol, stigmasterol and beta-sitosterol with a plurality of forms in tobacco leaves at the same time, has the advantages of accurate measurement and simple and convenient operation, and can be widely applied to tobacco industry.

This invention is improved laser ablation of solid samples analyzed by inductively coupled plasma (ICP), ICP mass spectrometry, or flowing afterglow mass spectrometry. A mirror-with-hole eliminates chromatic aberration in sample viewing and allows rad-hardening for radiation hot cell analysis of nuclear waste. Other attributes facilitate comprehensively rad-hardened laser ablation. Additional improvements include large, homogeneous laser spots, long-focus objective lenses, variable laser path length with built-in re-alignment, variable demagnification ratio, higher powered SMR lasers with larger spots enabling sensitive bulk solids analysis, demountable gravitationally self sealing stack assembly sample ablation cells, and laser ablation sample changers.

The invention relates to a method for detecting the plasticizer content in a solid sample by headspace solid phase microextraction gas chromatography-tandem mass spectrometry, and the method combines an isotope internal standard and the headspace solid phase microextraction gas chromatography-tandem mass spectrometry (HS-SPME-GC-MS/MS) analysis for qualitative or quantitative analysis of a dimethyl phthalate plasticizer in a sample. The method mainly comprises the following steps of: a) mixing the solid sample with the isotope internal standard, and conducting extraction with headspace solid phase microextraction equipment; b) carrying out sample analysis; C) performing GC-MS/MS analysis; and d) implementing result treatment and quantitative analysis. The method of the invention is a fast, efficient, sensitive analysis method.

The invention discloses a chromatograph on-line analysis method for source rock by closed ball milling, heating analysis and cold trap trapping, which is used for analyzing light fraction compound and percentage composition thereof in the dispersed organic matter of the source rock, and is characterized by comprising the following steps: carrying out vacuum sealing and ball milling on a source rock sample, carrying out ball milling, crushing, then heating to 300DEG C, and carrying out thermal desorption; blowing the pyrolysed hydrocarbon to a liquid helium cold trap by utilizing helium; placing the cold trap into a chromatograph furnace; and trapping, then carrying out chromatograph or Chromatographic-MS on-line analysis to obtain the qualitative data of chromatograph fine separation and/or Chromatographic-MS analysis of the light fraction in the source rock. The separation degree of the sample can approximate to the separation effect of the light dydrocarbon chromatograph separation of crude oil; the invention has good repeatability, reduces the volatilization loss of the light fraction in the crushing process because of the large volume of the sample before being put in a ball milling tank; and the qualitative analysis result can be used for researching the maturity and the environment of sedimentation of the source rock and carrying out oil source comparison.

The method and measurement system according to the invention performs combined Chromatography and Mass Spectrometry analysis and comprises the steps of: performing an C/MS analysis 300; generating at least one first elution profile 305, wherein one dimension is an elution time of the chromatography, and one dimension is mass to charge ratio (m/z), and at least one dimension a signal intensity, and the signal from each biomolecule species is dispersed forming a plurality of signal peaks associated with each biomolecule species in the elution profile; and reassembling 310 the dispersed signal originating from one biomolecule species in the elution profile. The reassembling comprises an automated annotation adapted to reassemble signal variations in the elution profile that originate from the same biomolecule species and generating a biomolecule map. The automated annotating is simultaneously based on both the elution time-dimension and the m/z-dimension.

A composition, method and device for the preparation of biological samples for subsequent instrumental analyses, such as GC, GC-MS, LC and LC-MS analysis, using a solid phase extraction (SPE) process is described. Through SPE process alone or an integrated combination of protein precipitation, filtration, and SPE using a hydrophobic zirconia-coated chromatographic media, interfering compounds, such as proteins, glycerides and phosphate-containing compounds, are eliminated from the biological, food, environmental and biotechnology samples, affording an enhanced analyte response during the instrumental analysis.

The invention relates to an extraction method of essential oil and selects two technological extraction ways depending on the difference of freshness of the Chinese torreya shell raw material. As regard to fresh Chinese torreya shells, namely the Chinese torreya shells stored for no more than 1 day under normal temperature and pressure, and a method of first cold pressing and then steam distillation can be adopted; as regard to stale Chinese torreya shells, namely the Chinese torreya shells stored for more than 1 day under normal temperature and pressure and serious browning occurs, a method of first drying, then grinding and finally steam distillation can be adopted. The oil yields of the Chinese torreya shell essential oils obtained by the two extraction methods are respectively 0.3-0.5 percent (v/w, wet basic weight) and 1-1.25 percent (v/w, dry basic weight). By GC-MS analysis and electronic nose comparison, the flavour and constituents of the two essential oils are quite similar. By adopting different extraction processes according to the difference of freshness of the raw materials, the invention finally utilizes the steam distillation technology to extract the Chinese torreya shell essential oil and the method is characterized by safety, convenience, high efficiency, low cost, etc.

A combination of “bottom up” and “top down” MS analysis of posttranslational modifications in complex proteins is described. The method comprises digestion of the protein with an enzyme that forms larger peptide fragments than trypsin (>3000 D), performing HPLC with the fragments and applying a new data acquisition strategy using on-line coupling with e.g. LTQ-FTMS, a hybrid mass spectrometer that couples a linear ion trap with a Fourier transform ion cyclotron resonance (FTICR) cell. The method is applied to analysis of posttranslational modifications of protein isoforms.