180results about "Spectrometer combinations" patented technology

A charge detection mass spectrometer includes an ion trap configured to store ions therein and to release stored ions therefrom, and an electrostatic linear ion trap (ELIT) array, in the form of at least two ELITs or ELIT regions each spaced apart from the ion trap, each ELIT or ELIT region including first and second ion mirrors and a charge detection cylinder positioned therebetween. The ion trap is controlled to release at least some of the stored ions to travel toward and into each of the ELITs or ELIT regions, and the first and second ion mirrors of each of the ELITs or ELIT regions is controlled in a manner which traps one or more ions traveling therein and causes the trapped ion(s) to oscillate back and forth between the first and second ion mirrors each time passing through and inducing a corresponding charge on the charge detection cylinder.

A method of mass spectrometry comprising: performing a first MSMS analysis in which ions having a first mass to charge ratio detected in a survey mode are fragmented or reacted and then mass analysed so as to obtain first MSMS mass spectral data; adding a mass to charge ratio detected in the first MSMS mass spectral data to an exclusion list; performing a second MSMS analysis in which ions having a second, different mass to charge ratio detected in the survey mode are fragmented or reacted, wherein the method comprises checking that the second mass to charge is not present on the exclusion list prior to performing the second MSMS analysis.

One mode of a chromatograph mass spectrometer according to the present invention includes: a measurement unit (1) that includes a chromatograph unit (1A) and a mass spectrometry unit (1B) capable of performing MS / MS analysis, and collects chromatograph mass spectrometry data having three dimensions of time, m / z, and a signal intensity by repeatedly performing the MS / MS analysis by data independent analysis in the mass spectrometry unit on a sample containing a compound separated by the chromatograph unit; a component detection unit (42) that detects a compound and a component corresponding to a partial structure of the compound by obtaining MS / MS spectra of a bar graph presentation based on chromatograph mass spectrometry data over a predetermined m / z range for a target sample, estimating precursor ion peaks in each of the MS / MS spectra, and selecting peaks based on a predetermined standard regarding an m / z direction in each of the MS / MS spectra and a predetermined standard regarding a time direction for peaks that can be considered to be identical or an identical group on the MS / MS spectra; a narrowing unit (43, 44) that narrows down components to be analyzed by performing screening using prior information on the detected component; and a composition estimation unit (45) that, by using m / z information corresponding to a narrowed down component, estimate a composition or a chemical formula of the component.

Methods are described for determining the amount of insulin in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying insulin in a biological sample utilizing purification methods coupled with tandem mass spectrometric or high resolution / high accuracy mass spectrometric techniques.

A system includes a first pre-separation device configured to perform a first pre-separation of precursor ions according to mobilities of the precursor ions and a second pre-separation device positioned downstream of the first pre-separation device configured to perform a second pre-separation of precursor ions based on a mass-to-charge ratio (m / z) of the precursor ions. The system further includes a mass spectrometer positioned downstream of the second pre-separation device configured to acquire mass spectra for precursor ions emitted from the second pre-separation device. The second pre-separation device is synchronized with the mass spectrometer such that an m / z range of precursor ions emitted from the second pre-separation device corresponds to a precursor m / z isolation window of the mass spectrometer.

Provided are methods for determining the amount of estradiol in a sample using mass spectrometry. The methods generally involve ionizing estradiol in a sample and detecting and quantifying the amount of the ion to determine the amount of estradiol in the sample.

Disclosed herein are methods and systems for individual ion mass spectrometry (I2MS). The methods and systems described herein parse I2MS data and allows for informed tandem mass spectrometry analyses by determining a target m / z that increases the yield of an intact mass corresponding to an analyte of interest while reducing the yield of additional detectable masses.

A method for multiple reaction monitoring using a mass spectrometer (106) is proposed. The method comprises i) measuring (128), using a mass spectrometer (106), multiple reaction monitoring transitions of both a quantifier and a qualifier of both an internal standard and an analyte using alternating multiple reaction monitoring, the alternating multiple reaction monitoring comprising at least three multiple reaction monitoring channel groups, one of the multiple reaction monitoring channel groups being measured at the respective theoretical m / z values of both the quantifier and the qualifier of both the internal standard and the analyte, and the two other multiple reaction monitoring channel groups being measured at respective m / z values shifted by a predetermined level higher and lower; ii) comparing (130), for at least two of the groups, at least two of the quantifier / qualifier ratios of the multiple reaction monitoring transitions of the internal standard with reference values from a database (126) using at least one processing device (120), the comparing including determining a deviation between the quantifier / qualifier ratio and the reference value; iii) determining (132), if at least one deviation of the quantifier / qualifier ratio is within at least one predetermined tolerance range, using a processing device (120), a measurement result from the measured multiple reaction monitoring transitions of the analyte and the internal standard, and, if not, rejecting (136) the measured multiple reaction monitoring transitions; and including.

A system includes a first pre-separation device configured to perform a first pre-separation of precursor ions according to mobilities of the precursor ions and a second pre-separation device positioned downstream of the first pre-separation device configured to perform a second pre-separation of precursor ions based on a mass-to-charge ratio (m / z) of the precursor ions. The system further includes a mass spectrometer positioned downstream of the second pre-separation device configured to acquire mass spectra for precursor ions emitted from the second pre-separation device. The second pre-separation device is synchronized with the mass spectrometer such that an m / z range of precursor ions emitted from the second pre-separation device corresponds to a precursor m / z isolation window of the mass spectrometer.

The invention discloses a sample introduction device for tandem mass spectrometry, and belongs to the technical field of compound detection devices.The sample introduction device comprises a partition ionization mechanism, the partition ionization mechanism comprises a cover body arranged on a conveying line, air cylinders are fixedly installed on the two sides of the top end of the cover body, piston rods on the air cylinders are connected to side plates, and the top end of the cover body is provided with a baffle; the bottom of the side plate penetrates through the cover body and extends to the conveying line; an ion source for ionizing a sample is mounted at the top end of the inner wall of the cover body; and the blade heat dissipation mechanism comprises a first motor fixed to the periphery of the cover body, and one end of an output shaft of the first motor extends to the first blade through a first bevel gear. The technical problems that a sensing and refrigerating system is complex in design, inconvenient to maintain and high in cost once being damaged, and the working effect of the whole refrigerating system is affected due to the fact that the temperature of a refrigerant medium can be reduced when the difference between the internal temperature and the external temperature of the environment is small, and the heat transfer efficiency is reduced can be solved.

Methods for detecting or determining the amount of an antidepressant and / or antidepressant metabolite in a sample are described. More particularly, mass spectrometry methods for detecting and quantifying an antidepressant and / or antidepressant metabolite in a sample are described.

Methods and apparatuses for the identification and / or characterization of properties of a macromolecule based on mass spectrometry data. Specifically, described herein are methods and apparatuses for converting peptide-level data into a pseudo-intact mass spectra. Also described herein are methods and apparatuses for converting peptide-level data into a pseudo-electropherogram. The methods may be well suited for analyzing proteins and protein complexes, including estimating properties of post-translational modifications of the proteins and protein complexes. Methods may include generating a theoretical graph or spectrum based on peptide-level mass spectrometry data. In some embodiments, the theoretical graph may be a theoretical intact mass spectrum or a theoretical charge distribution spectrum.

In an aspect, the present disclosure relates to a method. In one implementation, the method includes providing a material in an air-tight chamber defining an inlet and an outlet; supplying a direct current voltage to an electrode proximate to the material to generate ions in the air-tight chamber; introducing a carrier gas to the air-tight chamber at the inlet; and collecting the carrier gas and the ions at the outlet.

In one aspect, a system and method for calibrating a hybrid mass spectrometer is provided. The calibration may include an accurate mass calibration and, for example, a nominal mass calibration performed over multiple transmission widths and scan rates, ideally applied to each reference standard, where each reference standard represents a different m / z, to generate a matrix of correction factors corresponding to each of the transmission width and scan rate pairs at m / z values ​​for the reference standard being evaluated. Multi-parameter interpolation may be applied to identify correction factors to be used for subsequent analysis transmission width and scan rate pairs that differ from the multiple transmission widths and scan rates used to generate the correction factors.

Analyzer, which includes the following: an ion source (1); an ion mobility separation element (7) containing a pair of opposing electrodes (3, 4) to which a high-frequency voltage (13) and a direct current voltage (14) are applied; and a shielding electrode (2, 42, 43, 46) which is provided between the ion source (1) and the ion mobility separation part (7) and to which a DC voltage (12, 70) is applied, wherein the shielding electrode (2, 42, 43, 46) has an ion flow path (8, 41, 45, 47, 49) which connects an inlet (60), from which ions are introduced from the ion source (1), with an outlet (62), from which the ions are released, and the ion flow path (8, 41, 45, 47, 49) includes a cylindrical section which is bent in its middle so that the outlet (36) cannot be seen from the inlet (60).

Desorber 600 has body 610 which can be removably coupled to a sample inlet of a chemical trace detection system. Sample heater 640 can desorb a sample held in sample trap 630 coupled to sample holder

A method for multiple reaction monitoring using a mass spectrometer (106) is proposed. The method comprises i) measuring (128), using a mass spectrometer (106), multiple reaction monitoring transitions of both a quantifier and a qualifier of both an internal standard and an analyte using alternating multiple reaction monitoring, the alternating multiple reaction monitoring comprising at least three multiple reaction monitoring channel groups, one of the multiple reaction monitoring channel groups being measured at the respective theoretical m / z values of both the quantifier and the qualifier of both the internal standard and the analyte, and the two other multiple reaction monitoring channel groups being measured at respective m / z values shifted by a predetermined level higher and lower; ii) comparing (130), for at least two of the groups, at least two of the quantifier / qualifier ratios of the multiple reaction monitoring transitions of the internal standard with reference values from a database (126) using at least one processing device (120), the comparing including determining a deviation between the quantifier / qualifier ratio and the reference value; iii) determining (132), if at least one deviation of the quantifier / qualifier ratio is within at least one predetermined tolerance range, using a processing device (120), a measurement result from the measured multiple reaction monitoring transitions of the analyte and the internal standard, and, if not, rejecting (136) the measured multiple reaction monitoring transitions; and including.

To provide a system for performing multiplexed ion pre-separation for mass spectrometry.SOLUTION: The system includes a pre-separation device for separating the precursor ions into a set of discrete fractions of precursor ions based on a physical property of the precursor ions and for sequentially transmitting a first subset of the discrete fractions of precursor ions included in the set of discrete fractions of precursor ions. The system further includes a mass spectrometer positioned downstream of the pre-separation device for receiving the first subset of the discrete fraction of precursor ions. The mass spectrometer includes an ion storage for accumulating a first population of product ions generated from each discrete fraction of precursor ions included in the first subset of discrete fractions of precursor ions and a mass analyzer for performing mass analysis of the first population of product ions.SELECTED DRAWING: Figure 1

A method of mass and / or mobility spectrometry comprises: separating a sample with a chromatography device such that different analytes in the sample have different retention times in the chromatograph

Methods are provided for detecting the amount of one or more CAH panel analytes (i.e., pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, deoxycorticosterone, 11-deoxycortisol, and cortisol) in a sample by mass spectrometry. The methods generally involve ionizing one or more CAH panel analytes in a sample and quantifying the generated ions to determine the amount of one or more CAH panel analytes in the sample. In methods where amounts of multiple CAH panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.

The present teachings generally relate to generating a pseudo-barrier via an axial RF field. A first voltage is applied to a first pair of rods arranged in a multi-pole configuration, a second RF voltage is applied to a second pair of rods, and a difference between magnitudes and / or phases of the first and second RF voltages is adjusted. As discussed in more detail herein, such a pseudo-barrier may be employed in a variety of different applications. As an example, such a pseudo-barrier may be used to trap ions within a rod group that includes a plurality of rods arranged in a multi-pole configuration. In other applications, adjusting the height of the pseudo-potential barrier established in the rod set along with the application of a DC potential between the rod set and an external electrode can be used to cause mass-selective extraction of ions trapped within the rod set. In other applications, such a pseudo-barrier may be employed in a multiplexing method to perform mass spectrometry.

A method of mass spectrometry and / or mobility spectrometry includes: providing an ion separation device including a plurality of electrodes; providing a first force to urge the ions in a first direction along an axis of the ion separation apparatus while also providing a second force to urge the ions in a second, opposite direction such that ions of different mobility or mass-to-charge ratios reach different equilibrium positions at different locations along the axis; and varying the first force and / or the second force during a single elution cycle of the ion separation device so as to sequentially release ions from the ion separation device in an increase order of ion mobility or mass-to-charge ratio or in a decrease order of ion mobility or mass-to-charge ratio; wherein the altering step is performed such that, during the elution cycle, ions in a first range of mobility or mass-to-charge ratio are eluted from the ion separation device at a first rate and ions in a second, separate range of mobility or mass-to-charge ratio are eluted from the ion separation device at a second, different rate.

The invention relates to a method for separating ions and a storage ion mobility separator. The storage ion mobility separator comprises an ion guide extending in a closed loop and containing a substantially quiescent gas through which ions pass. An axial force acting on the ions is provided by an electric field barrier that provides an electric field gradient moving around the ion guide and has an effect on the movement of the ions that is dependent on the ion mobility.At least one operating parameter of the storage ion mobility separator that influences mobility separation is varied as a function of time such that the ions are pushed in a controlled manner along the moving electric field barrier towards a high potential end of the electric field barrier, with the ions being ejected laterally from the ion guide before sliding over the electric field barrier.

A sample is separated by a first separator into constituent analytes over a time parameter before further analysis by a mass spectrometer. Intensity measurements against mass-to-charge ratio for each

Mass spectrometric method (100) for detecting an analyte in a biological sample solution comprising the steps: providing (101) a constant stream of ionized sample molecules comprising the analyte from the biological sample solution and an analyte-specific internal standard including electrospray ionization (ESI), and / or nano-electrospray ionization (nano-ESI); performing (102a) a spatial accumulation of the analyte and the analyte-specific internal standard and performing (102b) at least partially a first separation of the analyte and the analyte-specific internal standard from other components of the ionized sample molecules based on the size and / or shape of the ionized molecules; performing (103) at least partially a second separation of the analyte and the analyte-specific internal standard from remained other components of the ionized sample molecules based on the mass to charge ratio of the ionized molecules; fragmenting (104) the ionized analyte into ionized analyte fragments and the analyte-specific internal standard into ionized analyte-specific internal standard fragments by collision with particles of a gas phase; providing (105) a stream comprising the ionized analyte fragments and the ionized analyte-specific internal standard fragments on a detection mass spectrometer; and recording (106) with the detection mass spectrometer at least two pre-summed mass-resolved spectra and summing (107) the at least two pre-summed mass-resolved spectra to obtain a final mass-resolved spectrum.

Oral compositions and methods for providing topical photoprotection against ultraviolet (UV) radiation are disclosed, wherein active photoprotective agents are designed, optimized, synthesized, and detected using artificial intelligence (AI) driven platforms. The invention encompasses AI-driven methods for designing novel photoprotective molecules (e.g., “Compound X-Sweat”) with specific physicochemical properties conducive to glandular excretion (via sweat and / or sebum) and optimized UV absorption characteristics. The invention further includes the novel AI-designed compounds themselves, plausible methods for their chemical synthesis, and synergistic photoprotective systems designed by AI for broad-spectrum coverage, multi-pathway excretion, and / or enhanced glandular excretion of photoprotective agents, with synergy being quantitatively defined. Additionally, methods for detecting and quantifying these agents on a subject's skin surface following oral administration are provided, serving as a companion diagnostic and means for verifying efficacy and infringement. The AI-designed agent(s), following oral administration, are excreted onto the skin surface, forming a protective layer or achieving a localized concentration effective to absorb or scatter UV radiation or provide antioxidant effects, thereby enabling a systemic route to persistent, broad-spectrum, and user-friendly photoprotection without the drawbacks of topical sunscreen reapplication.

An MS2 analysis for one precursor ion is performed to collect data on each micro area within a measurement target area (S1). A plurality of product ions are extracted based on those data (S2), and a mass spectrometric (MS) imaging graphic is created for each m / z of the product ion (S3). Hierarchical cluster analysis is performed on the created MS imaging graphics to group the product ions based on the similarity of the graphics (S4). Product ions having similar distributions are sorted into the same group. Such a group of ions can be considered to have originated from the same compound. Accordingly, the intensity information of a plurality of product ions is totaled in each group and for each micro area (S5), and an MS imaging graphic is created based on the totaled intensity information (S6). Even if there are a plurality of compounds overlapping the precursor ion, the influence of the overlapping can be eliminated through those steps. Thus, a graphic having a higher level of SN ratio, sensitivity and dynamic range than an MS imaging graphic obtained at a single product ion can be created and displayed.

The present teachings are generally directed to a method of performing mass spectrometry, the method comprising dissociating a plurality of precursor ions to generate a first set of product ions, where ions having substantially the same m / z are associated in one group, and where the first set of product ions comprises at least two groups of ions having different m / z. The method further includes introducing a first set of product ions into the ion trap; different groups of the first set of product ions are transferred from the ion trap to an ion dissociation device over different time intervals to dissociate at least a portion of the first set of product ions to generate a second set of product ions, and a mass spectrum of the second set of product ions is acquired.

A mass spectrometer comprises an ion source 10 (Figure 1), a mass pre‐filter 20 downstream of the ion source, a collision cell 30 (Figure 1), at least one mass filter downstream of the collision cell,