644results about "Mass spectrometric analysis" patented technology

The present invention relates to method for the direct detection and / or quantification of at least one compound with a molecular weight of at least 200, wherein the compound to be detected and / or quantified is a chemically complex molecule, wherein said chemically complex molecule is substituted with at least two groups —R, wherein each R group means independently —OH, —OP(O)(OH)2 or —P(O)(OH)2, with the proviso that at least two R are independently selected from —P(O)(OH)2 and —OP(O)(OH)2, wherein the compound or compounds to be detected and / or quantified are within a biological matrix, wherein said biological matrix is a biological fluid, a biological tissue, stomach contents, intestine contents, stool sample or a culture cells, wherein the method comprises performing a chromatography and identifying the retention time and / or the intensity of the signal by means of a mass or radioactivity detector.

The invention relates to the technical field of comparative proteomics, and in particular to a detection and quantification method for post-translational modification proteomics. The method is characterized in that equiponderous tandem mass tag labeling is carried out on a to-be-detected protein sample and an internal standard substance, and tandem mass spectral analysis is carried out on a labeled mixture, wherein the internal standard substance is a mixture which is rich in to-be-detected post-translational modification peptide fragments. According to the method, a signal containing the to-be-detected post-translational modification peptide fragments can be amplified, and therefore under the circumstances that the sensitivity of the mass spectrum is not changed and the post-translationalmodification peptide fragments do not need to be enriched, the probability that the post-translational modification peptide fragments are subjected to mass spectrometry detection and MS / MS analysis can be improved.