644results about "Mass spectrometric analysis" patented technology

The invention discloses a quantitative detecting method for various metabolic components in a biological sample, and a metabolic chip used for the quantitative detecting method. The quantitative detecting method is characterized in that the biological sample is subjected to derivatization treatment, and the biological sample subjected to derivatization is detected through liquid chromatography andmass spectrometry combination; and during derivatization treatment, 3-nitrophenylhydrazine serves as a derivatization reagent, and 1-(3-dimethylaminopropyl)-3-ethly carbodiimide serves as a derivatization reaction catalyst. According to the quantitative detecting method, high-sensitivity detection can be achieved, and a plurality of level-crossing metabolic components can be covered during detection, and the quantitative detecting method is simple, fast and suitable for being applied to clinical and scientific research testing. The metabolic chip comprises a chip carrier microtitration plateand related reagents, and thus the multiple level-crossing metabolites such as amino acid, phenols, phenyl or benzyl derivatives, indole, organic acid, fatty acid, sugar and bile acid in the biological samples can be subjected to quantitative detection on the same microtitration plate.

The present invention provides a method for the analysis of a compound with amino group (e.g., an amino acid or peptide) contained in a sample and convenient manner with a high sensitivity. The compound with amino group in a sample containing the compound with amino group is labeled with a specific carbamate compound such as p-trimethylammonium anilyl-N-hydroxysuccinimidyl carbamate iodide to enhance the selectivity and sensitivity. The present invention is preferably used in conjunction with mass spectrometry such as MS / MS method to facilitate quantitative analysis. The present invention further provides labeling reagents for mass spectrometry.

The present invention provides methods, compositions, and kits associated with analyzing, enriching, and / or isolating a biomarker or analyte in a biological sample. In one aspect, for example, a method for determining a concentration of a biomarker in a biological sample can include binding any unbound biomarker with an antibody specific for the biomarker to form antibody-bound biomarker, enriching the antibody-bound biomarker and any endogenous autoantibody-bound biomarker to form an enriched fraction, identifying the biomarker in the enriched fraction, and determining the concentration of the biomarker in the biological sample. In one aspect, the concentration of the biomarker is derived from initially unbound biomarker and autoantibody-bound biomarker in the biological sample.

The present invention relates to method for the direct detection and / or quantification of at least one compound with a molecular weight of at least 200, wherein the compound to be detected and / or quantified is a chemically complex molecule, wherein said chemically complex molecule is substituted with at least two groups —R, wherein each R group means independently —OH, —OP(O)(OH)2 or —P(O)(OH)2, with the proviso that at least two R are independently selected from —P(O)(OH)2 and —OP(O)(OH)2, wherein the compound or compounds to be detected and / or quantified are within a biological matrix, wherein said biological matrix is a biological fluid, a biological tissue, stomach contents, intestine contents, stool sample or a culture cells, wherein the method comprises performing a chromatography and identifying the retention time and / or the intensity of the signal by means of a mass or radioactivity detector.

The invention relates to the technical field of comparative proteomics, and in particular to a detection and quantification method for post-translational modification proteomics. The method is characterized in that equiponderous tandem mass tag labeling is carried out on a to-be-detected protein sample and an internal standard substance, and tandem mass spectral analysis is carried out on a labeled mixture, wherein the internal standard substance is a mixture which is rich in to-be-detected post-translational modification peptide fragments. According to the method, a signal containing the to-be-detected post-translational modification peptide fragments can be amplified, and therefore under the circumstances that the sensitivity of the mass spectrum is not changed and the post-translationalmodification peptide fragments do not need to be enriched, the probability that the post-translational modification peptide fragments are subjected to mass spectrometry detection and MS / MS analysis can be improved.