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Transgenic plants for nitrogen fixation

A technology for nitrogen-fixing microorganisms and plants, applied in plant peptides, plant products, genetic engineering, etc., can solve the problems of reducing the annual yield of crops and transferring nitrogen-fixing organic matter.

Inactive Publication Date: 2016-08-17
UNIV OF BREMEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, green manures cannot be planted together with other crops, which reduces the annual yield of crops per hectare
Furthermore, it has been found that it is currently not possible to transfer nitrogen-fixing organics to species that have not been adapted to co-cultivation with such microorganisms

Method used

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  • Transgenic plants for nitrogen fixation
  • Transgenic plants for nitrogen fixation
  • Transgenic plants for nitrogen fixation

Examples

Experimental program
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Effect test

Embodiment approach

[0117] It is to be noted that the term "a" or "an" entity refers to one or more of that entity; for example, "a plant cell" is understood to mean one or more plant cells. Likewise, the terms "a" (or "an"), "one or more" and "at least one" are used interchangeably herein.

[0118] Cultivars and cultivars, respectively, are systematic groups of cultivated plants whose characteristics are distinct, uniform and stable and which, when propagated by appropriate means, retain these characteristics. A cultivar group is a group of appropriately named cultivars based on one or more criteria. A cultivated plant is a plant whose origin or selection is primarily attributable to the purposive activities of man. Such plants may be produced by deliberate or unintentional crossing of cultivars, or by selection from existing cultivars, or such plants may be the result of selection from a few variants in wild populations and produced only by deliberate Continuous propagation of cultivated plan...

Embodiment 1

[0167] Example 1: Gene Mining

[0168] The following description summarizes the process of how to define putative plant candidate genes involved in high nitrogen fixation potential in Oryza longistamina.

[0169] Abbreviations: BNF—biological nitrogen fixation; EST—expressed sequence tag;

[0170] nifH - one of the structural genes encoding nitrogenase, the key bacterial enzyme for nitrogen fixation.

[0171] First RNA analysis :

[0172] Since there is currently no O. longista genome sequence available for gene mining, deep-sequencing of the Oryza longistamin root transcriptome was performed on plants adapted to high BNF rates in soil without nitrogen fertilization. In total, early next-generation sequencing (pyrosequencing) revealed 60,155 ESTs that could be aligned to the japonica or indica genome sequence, of which a remarkable 9.993 were genes that had not been detected to be expressed in rice. About 11,000 ESTs are potential novel sequence tags found only in our Ory...

Embodiment 2

[0288] Example 2: Estimation of nifH transcript abundance

[0289] Levels of NifH transcripts associated with plant roots were assessed from total RNA extracts from plant roots as previously published (Hurek et al., 2002) and modified (Burbano et al., 2011, supra). figure 1 In, DNA amplification of the nifH fragment was performed with similar primers as in Hurek et al., 2002. for figure 2 , Amplification of the nifH fragment was obtained by the universal primer set in the nested reverse transcription (RT)-PCR method (Burbano et al., 2011, supra): A nested RT-PCR protocol was used. The RT step was performed at 42°C using primer nifH3. PCR amplification was performed with primers nifH3 and nifH4 using annealing 55°C. A second round of nested PCR amplification was performed with 1 μl of the first round product and primers ZehrF and ZehrR. For this second round, quantitative real-time PCR was used for quantification (Bio-Rad CFX 96). The actin gene was used to normalize quan...

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Abstract

Provided are genetic material and nucleic acid sequences useful of increasing yield, biomass, growth rate, vigor, nitrogen use efficiency and / or abiotic stress tolerance, preferably tolerance to nutrient deficiency of a plant. Specifically, the improvement of nitrogen fixation properties in cultivated plants is described.

Description

technical field [0001] In general, the present invention relates to genetic material and nucleic acid sequences useful for increasing plant yield, biomass, growth rate, vigor, nitrogen use efficiency and / or abiotic stress tolerance, preferably tolerance to nutrient deficiency sex. In particular, the present invention relates to the improvement of nitrogen fixation performance in cultivated plants. The present invention relates to transgenic plants, methods for their manufacture and uses of such plants. In addition, the present invention relates to methods that can be used to make and evaluate such plants. In particular, the present invention belongs to the field of nitrogen fixation (nitrogen fixation) and allows imparting nitrogen-fixing properties or enhancing such properties in plants. Background technique [0002] Plant nutrients are essential for the growth and development of plants and therefore for the quantity and quality of plant products. Numerous fertilizers a...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H5/10
CPCC07K14/415C12N15/8271Y02A40/146C12N15/8261C12N9/0095C12Y118/06001C12Q1/6895C12Q2600/13C12Q2600/158G01N33/84G01N2333/415G01N2458/15G01N2560/00
Inventor 芭芭拉·莱因霍尔德-胡雷克托马斯·胡雷克胡利伟王祺杨海元
Owner UNIV OF BREMEN
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