431 results about "Nitrogen fixation" patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

The invention discloses plant probiotics with water retention, growth promotion and disease resistance effects and application thereof. The invention provides a complex microbial inoculant, which is prepared from the following active ingredients: 1) bacillus tequilensis Bac365 (GDMCC No: 65241), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) Enterococcus querebrasis Ent274 (GDMCC No: 65242) or Pseudomonas plecoglossa Pse350 (GDMCC No: 65243), and the active ingredients of the complex microbial inoculant are as follows: 1) bacillus tequilensis Bac365 (GDMCC No: 65243), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) the active ingredients of the complex microbial inoculant. The strain provided by the invention has good nitrogen fixation, phosphorus dissolution, IAA production, HA production and disease resistance, can effectively increase the utilization efficiency of water in drought soil, significantly promote plant growth and increase crop yield, and has good application prospects in the aspects of water retention and sand stabilization, agricultural production and the like.

The invention discloses bacillus for preventing and treating anthracnose of camellia oleifera and application of the bacillus. The preservation number of the bacillus CH-1-7 provided by the invention is GDMCC (Glucose Digital Microorganism Center) No. 66219; the bacillus amyloliquefaciens has the capability of generating living-promoting substances, can generate organic phosphorus and siderophores, has the nitrogen fixation capability, can inhibit the growth of various pathogenic bacteria, especially colletotrichum gloeosporioides, can be used for preventing and treating camellia oleifera anthracnose, and has important application value in the field of plant protection.

The invention belongs to the technical field of microorganisms, and relates to a strain of herbaspirillum as well as a fermentation method and application thereof. The invention provides a strain of brassica juncea, the brassica juncea is brassica juncea YLHb25.7, the brassica juncea is named as Herbaspiril huttiense, the brassica juncea is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35297. The invention also provides a preparation method of the brassica juncea. The herbispirillum sp. YLHb25.7 disclosed by the invention has the capabilities of producing ACC deaminase, producing IAA and fixing nitrogen, and has good drought resistance and saline-alkaline tolerance. The herbaspirillum sp. YLHb25.7 and the fermentation product thereof can promote the growth of wheat and corn, can be used as a microbial agent or bacterial fertilizer, and can be applied to the agricultural market.

The invention relates to an application of a nano silicon dioxide combined flora in relieving plant salt stress, which comprises the following steps: applying nano silicon dioxide to soil in which soybeans are to be planted, then planting the soybeans in the soil, and adding a simplified salt-tolerant growth-promoting synthetic flora microbial inoculum; wherein the synthetic flora is separated from saline-alkali soil, the growth promoting functions of nitrogen fixation, phosphorus dissolving, potassium dissolving and the like of the synthetic flora are measured, and the growth promoting effect of a single bacterium is verified through a pot experiment. A simplified flora SynC1 is constructed, and the relieving effect of combined application of the nano silicon dioxide and the flora on soybean salt stress is evaluated through a pot experiment. It is found that combined application of nano-silica and SynC1 can better promote root development, increase the proline content and promote soybean growth under salt stress, and has excellent application potential in agricultural soybean production.

The invention relates to the technical field of industrial microorganisms, and particularly discloses a P.megatherium HHY-1, a microbial agent thereof and application of the P.megatherium HHY-1. The preservation number of the P.megaterium HHY-1 is CGMCC (China General Microbiological Culture Collection Center) No.34470, and the preservation number of the P.megaterium HHY-1 is CGMCC No.34470. The P.megatherium provided by the invention has multiple functions of nitrogen fixation, phosphorus solubilization, siderophore production, IAA production capacity, antioxidant enzyme activity and the like, can play a comprehensive role from multiple dimensions of nutrition supply, growth regulation, stress resistance protection and the like, effectively assists good growth of the Abajie wheat 19 in a saline-alkali environment, and has a good application prospect. The method has great significance in improving the yield of the drought alkaline wheat in the saline-alkali soil and promoting agricultural sustainable development, and shows extremely high practical value in the aspect of growth promotion of the drought alkaline wheat Gie wheat 19.

The invention discloses a Klebsiella Michaeli MX-56 strain and application thereof, and belongs to the technical field of biological agriculture. The Klebsiella michelianensis MX-56 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 66361, the preservation time is May 19, 2025, and the preservation address is No. 100, Xianlie Middle Road, Guangzhou City. The invention also discloses an application of the Klebsiella michelianensis MX-56 in preparation of a divaricate saposhnikovia root growth promoting agent and a method for promoting growth of divaricate saposhnikovia root by using the Klebsiella michelianensis MX-56. A root irrigation method is adopted to irrigate MX-56 bacterial liquid. The divaricate saposhnikovia root endophytic bacterium Klebsiella michelianensis MX-56 disclosed by the invention has the growth promoting characteristics of fixing nitrogen, dissolving inorganic phosphorus, and secreting IAA (Indoleacetic Acid) and an iron-producing carrier; the strain can be stably colonized in the soil around the divaricate saposhnikovia roots; a good growth promoting effect is achieved on wind prevention.

The invention provides a method for repairing degraded moso bamboo forest soil. The method comprises the following steps: selecting a degraded moso bamboo forest; the moso bamboo forest is divided into a plurality of strip-shaped areas according to a high-density-low-density classification mode, and high-density areas and low-density areas which are distributed alternately are formed; the moso bamboo forest is subjected to strip-shaped thinning treatment, strip-shaped areas are divided according to a high-density-low-density classification mode, and moso bamboos of different growth ages are subjected to thinning according to a certain proportion; performing soil preparation treatment on the moso bamboo forest after thinning; planting nitrogen-fixing plants in the soil under the moso bamboo forest; and treating the soil under the moso bamboo forest by using a soil conditioner. According to the method for restoring the degraded moso bamboo forest soil, arbor layer-under-forest-soil integrated restoration is achieved, systematic improvement is conducted on multiple links of thinning, nitrogen fixation and fertilization, the content of N in the soil and the content of organic matter in the soil are increased, and the diameter at breast height of new bamboo is increased.

The invention discloses a preparation method and application of an iron-based metal organic framework material / carbon nitride nitrogen fixation photocatalyst in the field of photocatalytic materials, the iron-based metal organic framework material / carbon nitride nitrogen fixation photocatalyst is prepared by taking ferric chloride, terephthalic acid and graphite phase carbon nitride as raw materials through a hydrothermal reaction, a heterojunction is constructed through a solvothermal method, uniform loading of MIL-53 (Fe) on the surface of b-C3N4 is achieved, and the photocatalytic performance of the photocatalyst is improved. And the formed heterogeneous interface significantly improves the photon-generated carrier separation efficiency. The obtained MIL-53 (Fe) / b-C3N4 photocatalytic composite material has good visible light response characteristic and excellent photocatalytic nitrogen fixation performance, and is safe, non-toxic, green and environment-friendly. The produced ammonia water can be directly absorbed by wheat, so that the chlorophyll content of leaves is increased by 11-13%, the plant height is increased by 19-21%, and a novel photocatalysis scheme is provided for green agriculture.

The invention discloses a distributed plasma and electrocatalytic synthesis ammonia device which comprises a plasma nitrogen fixation unit, an electrocatalytic synthesis ammonia unit and a power supply module, and the plasma nitrogen fixation unit comprises an air pump, a plasma discharge reactor, a plasma power supply and an aeration head. The electro-catalytic ammonia synthesis unit comprises a cathode side liquid storage tank, a cathode side liquid pump, an ammonia production electrolytic bath, an anode side liquid pump, a direct-current power supply and an anode side liquid storage tank, and the power supply module is a clean energy module. The aeration head is immersed in the cathode side liquid storage tank, and electrolyte is stored in the cathode side liquid storage tank and the anode side liquid storage tank; the device takes air and water as raw materials and carries out a synthesis ammonia process at normal temperature and normal pressure. The method has the beneficial effects that the method adapts to clean energy, can realize on-site absorption and adapts to the volatility of the clean energy; raw materials are cheap and easy to obtain, hydrogen storage and transportation risks and extra cost pollution are avoided, and zero carbon is generated in the whole process; the device is small in scale and modularized, does not depend on large-scale facilities, and assists ammonia and chemical fertilizer production in regions deficient in resources.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plant's life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

The invention relates to the technical field of soil treatment, in particular to an integrated saline grainfield soil improvement and remediation method. According to the method, precise irrigation, concealed pipe drainage, salt-tolerant crop planting and bioremediation technologies are comprehensively applied, and systematic treatment of the salinized soil is achieved. Precise irrigation dynamically regulates and controls the leaching depth and frequency on the basis of real-time soil salinity and moisture data, the underground water level is intelligently controlled in combination with an underground pipe drainage network, salt accumulation is effectively reduced, and water resource waste is avoided; according to the salt-tolerant crop and green manure rotation mode, salt damage is reduced through plant salt absorption and root system nitrogen fixation, soil organic matter accumulation and microbial community reconstruction are promoted through application of a microbial agent and biochar, and a sustainable soil improvement mechanism is formed. The intelligent monitoring system integrates multiple types of sensors, collects key indexes such as salinity, nutrients and pH values in real time, predicts the salinization trend and regulates and controls irrigation, fertilization and drainage equipment in a linkage mode, full-process automatic management is achieved, and the management precision and efficiency are remarkably improved.

The invention discloses a special organic fertilizer for soybeans and a preparation method thereof, and belongs to the technical field of organic fertilizers. The special organic fertilizer for soybeans is prepared from the following raw materials in parts by weight: 8-10 parts of fish protein modified charcoal, 40-50 parts of decomposed soybean meal, 20-25 parts of decomposed livestock and poultry manure, 15-20 parts of humic acid, 10-15 parts of plant ash, 3-5 parts of a seaweed extract and 0.1-0.3 part of ammonium molybdate. According to the organic fertilizer, the soybean meal fermented by the trichoderma reesei and the aspergillus awamori and the fish protein modified biochar are added, the two components are compounded with the cooked livestock and poultry manure, the humic acid, the plant ash, the seaweed extract and the ammonium molybdate to form the organic fertilizer, the nitrogen fixation capacity of the soybeans is effectively improved through the synergistic effect of various substances, and the growth and development of the soybeans are remarkably promoted; the soybean yield and quality are improved. In addition, the whole organic fertilizer preparation process is free of strong acid and strong alkali treatment, the environmental pollution risk is reduced, and the standard of organic agriculture is met.

The invention discloses streptomyces obscura QS2-7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces caniferus QS2-7 is obtained through separation, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.32328. Experiments show that the strain QS2-7 has a relatively strong inhibition effect on various plant pathogens, and has excellent biocontrol bacterium potential. And the strain has the functions of nitrogen fixation, phosphorus solubilization and protease production, and can effectively degrade autotoxic substances generated by plant planting and relieve autotoxicity. The strain can significantly promote plant growth and effectively reduce the incidence rate of diseases, and has high application value in green prevention and control of plant diseases and continuous cropping production.

The invention discloses a soybean-corn intercropping nitrogen and phosphorus synergistic efficient utilization method based on rhizosphere signals, and relates to the technical field of microbial technology and agricultural engineering, a BX-pH dual sensor array is deployed in a root system intersection area, and the concentration and microscale pH change of benzoxazoline are monitored in real time; processing data by using two-stage feature transformation and a time sequence attention bidirectional GRU model, calculating a nitrogen-phosphorus disconnection coefficient and positioning risk coordinates; then, a buffer-antioxidant bifunctional nano-carrier is injected along the risk coordinates, neutralization acidification is carried out, and active oxygen is eliminated; after acidification and active oxygen signals are reduced to threshold values, DualFlow functional bacteria are inoculated at the same coordinate, and nutrition flow is recovered through phosphorus dissolution and nitrogen fixation synergistically; finally, a transport gene activation signal and nitrogen fixation intensity are continuously monitored through a BX-pH sensor array, and root zone water and proton outflow is adjusted in combination with variable-frequency pulse irrigation. Nitrogen and phosphorus utilization efficiency is remarkably improved, chemical fertilizer dependence is reduced, and crop yield and soil health are improved.

The invention discloses an application method for realizing rice nitrogen fertilizer substitution by utilizing a photocatalytic nitrogen fixation material in the technical field of agricultural production. The application method comprises the following steps: S1, pretreating and proportioning a catalyst; s2, reaction container deployment and illumination optimization; s3, carrying out photocatalytic reaction and ammonium nitrogen generation; s4, synergistically applying base fertilizer and additional fertilizer; s5, monitoring and feeding back nitrogen utilization efficiency; the performance of the photocatalyst is improved by adopting a surface doping and composite modification technology, atmospheric nitrogen can be stably and efficiently converted into ammonium nitrogen which can be directly absorbed by rice under a complex environmental condition, and the nitrogen conversion rate and the nitrogen supply efficiency are improved; the AI algorithm is combined with the chlorophyll meter, the soil nitrogen sensor and the water ammonia nitrogen concentration data to predict and dynamically adjust the nitrogen demand of the rice in real time; a closed loop of nitrogen fixation-conversion-absorption-recovery is formed through a segmented application strategy of base fertilizer and topdressing in combination with physical and chemical synergism and a biochar improvement technology.

The invention discloses a salt-alkali-tolerant growth-promoting ectopseudomonas DHS-HJ-IAA-2 strain and an application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of salt-resistant, alkali-resistant and drought-resistant pseudomonas sp. DHS-HJ-IAA-2 is screened, and the pseudomonas sp. DHS-HJ-IAA-2 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36164. Experimental results show that the ectopseudomonas DHS-HJ-IAA-2 has the functions of producing IAA, fixing nitrogen, dissolving potassium, degrading cellulose and lignin, inhibiting phytopathogen and the like, and can remarkably improve the bacteria abundance of saline-alkali soil, improve soil fertility and promote plant growth in a saline-alkali environment. The invention provides a new strain resource and a technical means for improving saline-alkali soil and planting crops on the saline-alkali soil, and realizes improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action.

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus amyloliquefaciens QGFCH3-3 as well as a microbial agent and application of the bacillus amyloliquefaciens QGFCH3-3. The preservation number of the bacillus amyloliquefaciens QGFCH3-3 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.34814 The strain has the functions of nitrogen fixation, amylase production, ligninase production, protease production and cellulase production, can effectively promote plant growth, has broad-spectrum antibacterial activity, can significantly inhibit various pathogenic bacteria, and significantly improves the disease control effect of crops.

The invention discloses an efficient composite intercropping method for pomegranate, dry rice and broad bean grain, which scientifically combines pomegranate (economic output), dry rice (grain output) and broad bean (partial economic output and biological nitrogen fixation and land cultivation), realizes functional complementation, and breaks through the single target limitation of the traditional intercropping mode. Through effective connection of crops for rotation of'broad bean sowing in winter and upland rice sowing in spring ', full utilization of light and heat resources and land in the orchard from winter to spring, summer and autumn in the next year is realized, and the multiple cropping index is remarkably improved; the pomegranate utilizes the upper-layer three-dimensional space, and the upland rice and the broad beans utilize the middle-lower-layer space, so that an efficient three-dimensional lighting structure and space combination are formed; the nitrogen fixation effect of the rhizobium of the broad beans can directly increase the nitrogen nutrition of the soil, the straw returning can increase the organic matters of the soil, and the built-in fertilizing mechanism of the orchard effectively relieves the consumption of the intercropping system on the soil fertility and ensures the sustainability of the mode.

The present invention relates to a (Rhodopseudomonas telluris DAEJI-NZ23 strain with accession number KACC81302BP, which exhibits nitrogen fixation capability, produces indole acetic acid (IAA), and has activities of reducing greenhouse gas, promoting plant growth, and secreting extracellular enzymes, and uses thereof. The strain of the present invention can be advantageously utilized in the technical field of greenhouse gas reduction, and is expected to increase crop yield while reducing excessive use of agricultural chemicals.

The invention discloses a method for enhancing the biological nitrogen fixation capacity of soil based on micro-plastics, which comprises the following steps: adding poly (adipic acid-butylene terephthalate) micro-plastics into target soil, and the addition amount is 0.2-2% of the mass of the soil. The activity of dehydrogenase and urease of rhizosphere microorganisms is activated through the degradation process of the poly (butylene adipate-co-terephthalate) micro-plastic in soil, and microorganism groups with a nitrogen fixation function are specifically enriched, so that the expression of nitrogen fixation genes is up-regulated, the biological nitrogen fixation capacity of the soil is enhanced, and the nitrogen condition of the soil is improved.

The invention discloses a growth-promoting halophilic Clariella sackii BWT 1.2507 strain and a preparation method of the growth-promoting halophilic Clariella sackii BWT 1.2507 strain. The growth-promoting halophilic Clariella sackii BWT 1.2507 strain has the advantages that the growth- The invention relates to a bacillus subtilis BWT1.2507, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on September 28, 2025, the address of the GDMCC is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong province, the postcode is 510070, and the preservation number is GDMCC No: 67048. The halophilic Sacchaffia halmapaa BWT1.2507 is separated and screened from the corn rhizosphere saline-alkali soil, has the functional characteristics of saline-alkali resistance growth, nitrogen fixation, organic phosphorus dissolution, IAA secretion and siderophore production, has obvious application effects in promotion of healthy growth of corn plants and alkali and salt reduction of saline-alkali soil, can be developed as an agricultural microbial agent, and has broad application prospects. The application potential in improvement of saline-alkali soil is huge.

The invention discloses a moss-microorganism synergistic nitrogen fixation bionic regreening method and system for a drought-resistant mine rock slope, and belongs to the technical field of ecological environment restoration. The method comprises the following steps: (1) slope pretreatment: drilling anchoring holes; (2) layered construction: (1) a fixation layer: spraying mixed slurry prepared from amylase and plant fibers to form a net-shaped substrate with the thickness of 1-2cm; (2) a nutrient layer: spraying a mixture of a complex microbial inoculant and a slow release fertilizer, wherein the thickness is 4cm; the mixed bacterial agent is prepared from the following raw materials in percentage by weight: 60 percent of phytonimum patenum spores, 25 percent of nostoc commune (Nostoc commune) and 15 percent of pseudomonas fluorescens (Pseudomonas fluorescens), and the mixed bacterial agent is prepared from the following raw materials in percentage by weight: 60 percent of phytonimum patenum spores, 25 percent of nostoc commune (Nostoc commune) and 15 percent of pseudomonas fluorescens (Pseudomonas fluorescens). And (3) a water retention layer: sodium polyacrylate resin is spread, the particle size of the sodium polyacrylate resin is 1-0.5 mm, soil covering and compaction are performed until the thickness of the water retention layer is 1-1.5 cm, and the use amount of the sodium polyacrylate resin is 2-1.5 wt% of the weight of the soil. According to the method, the nitrogen fixation efficiency can be improved by 40-60%, and the soil moisture content is kept gt under the drought condition; the survival rate of the seedlings is increased to 85% or above, the anti-scouring capacity of the side slope is improved by 50%, and the vegetation coverage rate of 70% or above is achieved within 6 months.

The invention provides an artificial non-coding RNA (Ribonucleic Acid) molecule, a DNA (Deoxyribose Nucleic Acid) molecule, a biological material and application of the artificial non-coding RNA molecule, the DNA molecule and the biological material in improving the carbon and nitrogen metabolism capability of rhizobium, and belongs to the technical field of genetic engineering, the nucleotide sequence of the artificial non-coding RNA molecule is as shown in SEQ ID NO.1, and the nucleotide sequence of the DNA molecule transcribing the artificial non-coding RNA molecule is as shown in SEQ ID NO.2; the invention also provides a recombinant vector and recombinant rhizobium with efficient carbon and nitrogen metabolism capability. According to the artificial non-coding RNA molecule and the recombinant expression vector constructed by using the RNA molecule, the utilization capacity of malic acid of the rhizobium can be remarkably improved, then the symbiotic nitrogen fixation capacity of the rhizobium is enhanced, the rhizobium and leguminous crops are symbiotic, and the yield and quality of the leguminous crops can be remarkably improved.

The invention relates to the technical field of microorganisms, in particular to growth-promoting pantoea TY-88 and application thereof in promotion of waxy corn seed germination and seedling growth, the preservation unit of the growth-promoting pantoea TY-88 is China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025945, and the preservation date is April 30, 2025. The growth-promoting pantoea TY-88 provided by the invention has better capabilities in the aspects of phosphate solubilization, nitrogen fixation, potassium solubilization and siderophore production; when the biological agent prepared from the growth-promoting pantoea is used for seed soaking treatment, waxy corn seed germination, seedling growth and development and substance accumulation of the overground part and the underground part can be remarkably promoted, and economic benefits are improved on the premise that the environment is not polluted.

The application belongs to the technical field of genetic engineering, and particularly relates to a soybean unconventional small peptide miPEP160b, a gene and application thereof. The application provides a soybean unconventional small peptide miPEP160b, and an amino acid sequence of the soybean unconventional small peptide miPEP160b is shown as SEQ ID NO. 2. The soybean unconventional small peptide miPEP160b can regulate symbiotic nodule formation of legume plants, can be used as a gene resource for biological breeding, and can be used for cultivating new varieties of legume plants with improved nodule formation ability and nitrogen fixation efficiency by using biological materials related to the soybean unconventional small peptide miPEP160b, thereby providing a new idea for soybean biological breeding and agricultural production application. In addition, the soybean unconventional small peptide miPEP160b can be used as a plant stimulant, can promote nodule formation ability and nitrogen fixation efficiency of soybeans by spraying around the roots of drip irrigation soybeans, and can also promote growth and development of soybean plants.

The application discloses a kind of based on near infrared spectrum to legume nitrogen fixation evaluation method, specifically related to computational formula processing method, including the following steps: S01, based on selected different plants to be measured sample, establish the standard sample set of PLSR model;S02, determine the target area of the to-be-tested sample detection, and obtain the spectral characteristics of target area by remote sensing image;S03, the standard sample set in the target area is scanned by near infrared spectrum, to obtain spectral data;S04, the spectral characteristics is combined with the spectral data, to obtain the fusion set of remote sensing image and spectral data;S05, the nitrogen content ([N]) and nitrogen isotope ratio (delta 15 N) in the standard sample set are acquired, to obtain the chemical measurement data corresponding to the to-be-tested sample of the different plants.The application realizes the efficient, non-destructive detection of the amount of biological nitrogen fixation of legume, compared with traditional chemical reagent and cumbersome experimental steps, has the advantages of simple operation, rapid detection, reliable result etc.