268 results about "Nitrogen fixation" patented technology

The invention belongs to the technical field of microorganisms, and relates to a strain of herbaspirillum as well as a fermentation method and application thereof. The invention provides a strain of brassica juncea, the brassica juncea is brassica juncea YLHb25.7, the brassica juncea is named as Herbaspiril huttiense, the brassica juncea is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35297. The invention also provides a preparation method of the brassica juncea. The herbispirillum sp. YLHb25.7 disclosed by the invention has the capabilities of producing ACC deaminase, producing IAA and fixing nitrogen, and has good drought resistance and saline-alkaline tolerance. The herbaspirillum sp. YLHb25.7 and the fermentation product thereof can promote the growth of wheat and corn, can be used as a microbial agent or bacterial fertilizer, and can be applied to the agricultural market.

The invention relates to the technical field of soil treatment, in particular to an integrated saline grainfield soil improvement and remediation method. According to the method, precise irrigation, concealed pipe drainage, salt-tolerant crop planting and bioremediation technologies are comprehensively applied, and systematic treatment of the salinized soil is achieved. Precise irrigation dynamically regulates and controls the leaching depth and frequency on the basis of real-time soil salinity and moisture data, the underground water level is intelligently controlled in combination with an underground pipe drainage network, salt accumulation is effectively reduced, and water resource waste is avoided; according to the salt-tolerant crop and green manure rotation mode, salt damage is reduced through plant salt absorption and root system nitrogen fixation, soil organic matter accumulation and microbial community reconstruction are promoted through application of a microbial agent and biochar, and a sustainable soil improvement mechanism is formed. The intelligent monitoring system integrates multiple types of sensors, collects key indexes such as salinity, nutrients and pH values in real time, predicts the salinization trend and regulates and controls irrigation, fertilization and drainage equipment in a linkage mode, full-process automatic management is achieved, and the management precision and efficiency are remarkably improved.

The invention discloses a salt-alkali-tolerant growth-promoting ectopseudomonas DHS-HJ-IAA-2 strain and an application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of salt-resistant, alkali-resistant and drought-resistant pseudomonas sp. DHS-HJ-IAA-2 is screened, and the pseudomonas sp. DHS-HJ-IAA-2 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36164. Experimental results show that the ectopseudomonas DHS-HJ-IAA-2 has the functions of producing IAA, fixing nitrogen, dissolving potassium, degrading cellulose and lignin, inhibiting phytopathogen and the like, and can remarkably improve the bacteria abundance of saline-alkali soil, improve soil fertility and promote plant growth in a saline-alkali environment. The invention provides a new strain resource and a technical means for improving saline-alkali soil and planting crops on the saline-alkali soil, and realizes improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action.

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus amyloliquefaciens QGFCH3-3 as well as a microbial agent and application of the bacillus amyloliquefaciens QGFCH3-3. The preservation number of the bacillus amyloliquefaciens QGFCH3-3 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.34814 The strain has the functions of nitrogen fixation, amylase production, ligninase production, protease production and cellulase production, can effectively promote plant growth, has broad-spectrum antibacterial activity, can significantly inhibit various pathogenic bacteria, and significantly improves the disease control effect of crops.

The invention discloses an efficient composite intercropping method for pomegranate, dry rice and broad bean grain, which scientifically combines pomegranate (economic output), dry rice (grain output) and broad bean (partial economic output and biological nitrogen fixation and land cultivation), realizes functional complementation, and breaks through the single target limitation of the traditional intercropping mode. Through effective connection of crops for rotation of'broad bean sowing in winter and upland rice sowing in spring ', full utilization of light and heat resources and land in the orchard from winter to spring, summer and autumn in the next year is realized, and the multiple cropping index is remarkably improved; the pomegranate utilizes the upper-layer three-dimensional space, and the upland rice and the broad beans utilize the middle-lower-layer space, so that an efficient three-dimensional lighting structure and space combination are formed; the nitrogen fixation effect of the rhizobium of the broad beans can directly increase the nitrogen nutrition of the soil, the straw returning can increase the organic matters of the soil, and the built-in fertilizing mechanism of the orchard effectively relieves the consumption of the intercropping system on the soil fertility and ensures the sustainability of the mode.

The invention discloses a growth-promoting halophilic Clariella sackii BWT 1.2507 strain and a preparation method of the growth-promoting halophilic Clariella sackii BWT 1.2507 strain. The growth-promoting halophilic Clariella sackii BWT 1.2507 strain has the advantages that the growth- The invention relates to a bacillus subtilis BWT1.2507, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on September 28, 2025, the address of the GDMCC is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong province, the postcode is 510070, and the preservation number is GDMCC No: 67048. The halophilic Sacchaffia halmapaa BWT1.2507 is separated and screened from the corn rhizosphere saline-alkali soil, has the functional characteristics of saline-alkali resistance growth, nitrogen fixation, organic phosphorus dissolution, IAA secretion and siderophore production, has obvious application effects in promotion of healthy growth of corn plants and alkali and salt reduction of saline-alkali soil, can be developed as an agricultural microbial agent, and has broad application prospects. The application potential in improvement of saline-alkali soil is huge.

The invention relates to the technical field of microorganisms, in particular to growth-promoting pantoea TY-88 and application thereof in promotion of waxy corn seed germination and seedling growth, the preservation unit of the growth-promoting pantoea TY-88 is China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025945, and the preservation date is April 30, 2025. The growth-promoting pantoea TY-88 provided by the invention has better capabilities in the aspects of phosphate solubilization, nitrogen fixation, potassium solubilization and siderophore production; when the biological agent prepared from the growth-promoting pantoea is used for seed soaking treatment, waxy corn seed germination, seedling growth and development and substance accumulation of the overground part and the underground part can be remarkably promoted, and economic benefits are improved on the premise that the environment is not polluted.

The application belongs to the technical field of genetic engineering, and particularly relates to a soybean unconventional small peptide miPEP160b, a gene and application thereof. The application provides a soybean unconventional small peptide miPEP160b, and an amino acid sequence of the soybean unconventional small peptide miPEP160b is shown as SEQ ID NO. 2. The soybean unconventional small peptide miPEP160b can regulate symbiotic nodule formation of legume plants, can be used as a gene resource for biological breeding, and can be used for cultivating new varieties of legume plants with improved nodule formation ability and nitrogen fixation efficiency by using biological materials related to the soybean unconventional small peptide miPEP160b, thereby providing a new idea for soybean biological breeding and agricultural production application. In addition, the soybean unconventional small peptide miPEP160b can be used as a plant stimulant, can promote nodule formation ability and nitrogen fixation efficiency of soybeans by spraying around the roots of drip irrigation soybeans, and can also promote growth and development of soybean plants.

The invention belongs to the technical field of environmental microorganisms, and provides Klebsiella capable of degrading thifensulfuron methyl and simultaneously promoting plant growth, the Latin of the Klebsiella is Klebsiella sp., the preservation name is Klebsiella sp. HDJY-1, the preservation place is China Center for Type Culture Collection, the preservation date is November 17, 2025, and the preservation number is CCTCC NO: M 20252578. The strain has excellent low temperature resistance and alkali resistance, not only can grow and propagate by taking thifensulfuron methyl as a unique carbon source and energy, but also can secrete plant growth-promoting substances such as indoleacetic acid and the like, and has a nitrogen fixation function. Therefore, the strain provided by the invention not only can remarkably promote plant growth and improve nutrient absorption efficiency so as to improve plant biomass and agricultural product quality, but also can provide an excellent strain resource for thifensulfuron methyl pollution remediation of farmland soil.

The application provides a kind of nano hollow microsphere photocatalytic material and its preparation method and application, [Bmim]I Except as iodine source, also as template agent, to the formation process of BiOI microsphere has played a key role. Then BiOI microsphere is calcined at 380 DEG C, makes it convert into BiOI / Bi5O7I further improves photocatalytic performance. BiOI / Bi5O7I microsphere shows more superior photocatalytic nitrogen fixation performance under visible light. In addition, BiOI microsphere shows more superior photocatalytic nitrogen fixation performance under visible light. The catalyst preparation method is simple and easy to operate, can be used for photocatalytic efficient nitrogen fixation, catalyst is easy to recycle.

The invention provides curtobacterium wilt S5 with efficient ultraviolet resistance and application of the curtobacterium wilt S5, and belongs to the technical field of agricultural microorganisms. According to the present invention, the curtobacterium wilting S5 is a natural high ultraviolet resistance bacterium screened from the extreme environment of the Qinghai plateau, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.37175; the strain not only can promote nitrogen fixation of plants, but also can improve the resistance of the plants to ultraviolet rays by absorbing the ultraviolet rays, and has the functions of stress resistance and yield increase. The brevibacterium wilt S5 strain disclosed by the invention can be applied to regions with high ultraviolet radiation to replace chemical protective agents, has no residual pollution, and is suitable for organic agriculture.

The application belongs to the technical field of microorganisms, and particularly relates to a halophilic bacillus, a bacterial agent and application thereof. The application provides a halophilic bacillus Halobacillus andaensis, and the preservation number is CCTCC M 20232394. The application also provides a bacterial agent containing the halophilic bacillus. The application also provides application of the halophilic bacillus or the bacterial agent in promoting plant growth under saline-alkali stress, improving plant stress resistance, improving soil, and crop production. The halophilic bacillus provided by the application has excellent saline-alkali tolerance, and can only grow under saline-alkali conditions. Meanwhile, the halophilic bacillus has growth-promoting characteristics such as salt reduction, acid production for alkali reduction, nitrogen fixation, and the like, can reduce salt damage, and improve crop yield and quality.

The invention provides application of beta-1, 4-glucan type cellooligosaccharide in improvement of plant rhizosphere micro-ecology and enhancement of IAA signals, and belongs to the technical field of biological agriculture. The invention provides an application of beta-1, 4-glucan type cellooligosaccharide with the polymerization degree of DP2-DP5 in improvement of plant rhizosphere micro-ecology and enhancement of IAA (Indole Acetic Acid) signals. In the invention, beta-1, 4-glucan type cellooligosaccharide can induce plant immunity in a growth allowable PTI threshold range, so that a rhizosphere microflora structure is directionally remodeled, the relative abundance of beneficial microorganisms is improved, the potential pathogen load is reduced, rhizosphere IAA and related metabolites thereof can be increased, and'disease resistance without growth inhibition 'is realized. The invention further provides an application of the beta-1, 4-glucan type cellooligosaccharide combined with rhizosphere beneficial microorganisms in biological agriculture, and growth promotion and / or nodulation nitrogen fixation effects can be enhanced.

The application of nano-silicon dioxide combined with a bacterial community in relieving plant salt stress, nano-silicon dioxide is applied to the soil where soybeans are to be planted, then the soybeans are planted in the soil, and a simplified salt-tolerant growth-promoting synthetic bacterial community inoculant is added; wherein the synthetic bacterial community is isolated from saline-alkali soil and its growth-promoting functions such as nitrogen fixation, phosphorus solubilization and potassium solubilization are determined, and the growth-promoting effect of single bacteria is verified through pot experiment. The construction of a simplified bacterial community SynC1 evaluates the relieving effect of nano-silicon dioxide and bacterial community combined application on soybean salt stress through pot experiment. It is found that the combined application of nano-silicon dioxide and SynC1 can better promote root development, increase proline content and promote the growth of soybeans under salt stress, and has excellent application potential in agricultural soybean production.

The application discloses a method for promoting paddy field biological nitrogen fixation based on long-distance electron transfer, and comprises the following steps: S1, soil pretreatment, establishing a flooding system; S2, pre-incubation to form a stable redox stratification; S3, constructing a long-distance electron transfer channel; S4, cultivation: under the conditions of flooding and a certain temperature, continue to cultivate to promote the paddy field biological nitrogen fixation in the flooding system. The application does not need an external power supply, only by arranging a graphite rod in the flooded paddy field soil, making it span the surface layer oxidation zone and the lower layer reduction zone, forming a long-distance electron transfer channel, thereby realizing the directional transport and utilization of the lower layer reduction equivalent to the surface layer, providing continuous support for the surface layer nitrogen fixation process from the electron supply end, promoting the nitrogen fixation process, and also promoting the enrichment of paddy field biological nitrogen fixation related microbial groups in the surface layer oxidation zone and changing the iron valence state distribution, promoting the transformation of Fe(III) into Fe(II), and enhancing the coupling of iron reduction and nitrogen fixation process.

The invention relates to a mung bean cultivation method suitable for arid regions, and belongs to the technical field of mung bean planting. Comprising the following steps: S1, selecting a dry farming plot with flat terrain for deep ploughing and soil preparation; s2, selecting a mung bean precision seeder provided with a linkage type liquid injection system; s3, the mung bean precision seeder is used for operation, and the following operation is executed in the advancing process of the seeder: S4, within 0.5 s to 1 s after the completion of S3, a soil covering and pressing mechanism arranged behind the seeder is used for covering soil on the seed furrows, a full-biodegradable mulching film is synchronously laid, and the high-humidity soil balls are sealed under the film. Ineffective evaporation of water is reduced to the greatest extent, and a micro-ecological environment which is beneficial to rapid germination of mung bean seeds in a drought and cold environment and establishment of a symbiotic nitrogen fixation system is established.

This invention discloses an efficient cultivation method for crop rotation of mung beans and Chinese cabbage. The method includes: (1) treating spring mung beans with seed priming one week before sowing, sowing in late March, harvesting in early June, and returning the straw to the field after crushing; (2) sowing seedling Chinese cabbage in mid-June and harvesting in late July; (3) sowing summer mung beans in late July and harvesting in early October, and returning the straw to the field after crushing; (4) sowing Chinese cabbage in mid-September for seedling cultivation, transplanting in mid-October, and maturing in late December. This invention adopts a scientific and reasonable crop rotation model, which can achieve four crops and four harvests a year, significantly improving land utilization. By planting mung beans for nitrogen fixation and returning straw to the field, soil fertility is improved, and the amount of fertilizer used for vegetable cultivation is reduced. By combining the planting of miscellaneous grains with vegetable cultivation, food security and vegetable supply are ensured, achieving significant economic, social, and ecological benefits.

The invention provides a penicillium oxalicum strain K3R2 and a preparation method and application of a biological agent of the penicillium oxalicum strain K3R2. The biocontrol bacterium Penicillium oxalicum strain K3R2 screened by the invention is preserved in the China General Microbiological Culture Collection Center on September 25, 2025, the preservation number is CGMCC NO.42219, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing; the ITS sequence of the Penicillium oxalicum strain K3R2 is as shown in SEQ ID NO: 1, and the CaM sequence is as shown in SEQ ID NO: 2. The penicillium oxalicum strain K3R2 provided by the invention can inhibit hypha growth and spore germination of beet neocam spores, the hypha inhibition rate is 83.60%, the spore inhibition rate is 65.31%, and the penicillium oxalicum strain K3R2 has the effect of preventing and treating quinoa stem blight. Meanwhile, the penicillium oxalicum strain K3R2 has the capabilities of salt resistance, alkali resistance, phosphate solubilization, potassium solubilization, cellulose solubilization and nitrogen fixation; the strain K3R2 remarkably promotes the growth of quinoa plants, so that the chlorophyll content is increased by 20.22%, and the accumulation of nutrient substances is accelerated; the fresh weight, the dry weight and the stem diameter of quinoa plants are increased, and the lodging resistance is enhanced.

The invention relates to the field of acid soil conditioners, in particular to a charcoal-based acid soil conditioner as well as a preparation method and application thereof. The biochar-based acid soil conditioner comprises a biochar carrier and a complex microbial inoculant loaded on the biochar carrier, and the complex microbial inoculant comprises 25-35 parts of nitrogen-fixing functional bacteria; 20 to 40 parts of phosphorus and potassium solubilizing bacteria; 15 to 25 parts of nitrogen activating bacteria; 15 to 25 parts of synergistic growth promoting bacteria; the nitrogen-fixing functional bacteria comprise Azospirillum brasilense and / or Klebsiella, the phosphate-solubilizing and potassium-solubilizing bacteria comprise bacillus megatherium and / or bacillus mucilaginosus, the nitrogen-activating bacteria comprise urease-producing bacillus and / or nitrospirillum, and the synergistic growth-promoting bacteria comprise arbuscular mycorrhizal fungi and / or pseudomonas. The complex microbial inoculant is constructed by screening four functional strains of nitrogen fixation, phosphorus and potassium solubilization, nitrogen activation and synergistic growth promotion, a complete functional closed loop of nitrogen fixation, phosphorus solubilization, nutrient activation and crop growth promotion is formed, and synergistic interaction is achieved through functional complementation of the strains.

The application provides a Lecanicillium attenuatum strain and application thereof, the classification and name of the strain are LIO314, which has been preserved in the China Center for Type Culture Collection on June 18, 2025. Lecanicillium aphanocladii The address for preservation is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20251419. Research shows that the strain exhibits significant inhibitory effect on plant pathogenic fungi such as Fusarium graminearum and Colletotrichum gloeosporoides, can efficiently kill pepper aphids, has nitrogen fixation, potassium and starch decomposition activities, can significantly promote the growth of rice, wheat and lettuce seedlings, can be used as an efficient, environment-friendly and low-cost biological control agent, simultaneously has plant growth regulation function, can be used as a biological bacteria organic fertilizer, and has wide development and utilization value and application prospect in agricultural production.

The application discloses an application method of leguminous green manure combined with growth-promoting bacteria for emission reduction and efficiency increase, and relates to the technical field of crop planting. The method comprises the following steps: sowing black bean seeds in soil inoculated with bacillus velezensis, and culturing black bean plants to the full flowering stage, and then crushing and turning over the black bean plants as green manure. A special bacillus velezensis is separated, which has the effects of bacteriostasis, nitrogen fixation, phosphorus dissolution, potassium solution, biofilm formation and auxin production, and has excellent biological characteristics such as stress resistance, rapid growth and easy separation, and the advantages of easy cultivation, safety, harmlessness and no pollution. Meanwhile, the bacillus velezensis can ferment to produce rich metabolic products, has good antibacterial activity and stress resistance. The bacillus velezensis is particularly suitable for being combined with black beans for green manure cultivation, and can maximize the effects of soil fertilization and gas emission reduction of green manure.

The application provides a micro-channel gas-liquid two-phase discharge nitrogen fixation device, which comprises a gas supply system and a liquid supply system, further comprises a micro-fluid chip and a dielectric barrier discharge system, the micro-fluid chip comprises a planar substrate and a transverse channel and a longitudinal channel which are arranged in communication along the planar substrate and are connected with the gas supply system and the liquid supply system respectively, wherein the gas-liquid two-phase forms a two-phase region in the longitudinal channel, and the dielectric barrier discharge system comprises a positive electrode and a negative electrode which are superimposed on the micro-fluid chip along the two-phase region to form an electric field. The micro-channel discharge of the application greatly improves the DBD discharge energy density and promotes the nitrogen dissociation; the micro-channel generates a stable gas-liquid two-phase flow pattern, strengthens the interface reaction and mass transfer between the plasma gas bubble group and the liquid phase, promotes the reaction equilibrium to move to the nitrogen fixation direction, so as to realize the improvement of the plasma nitrogen fixation energy efficiency.

The application discloses application of eugenol in preparation of a bacteriostatic agent for enhancing symbiotic nitrogen fixation efficiency of legume-rhizobium, and relates to the field of agricultural microorganisms. ‑1 The application is safe and non-inhibiting to seed germination of Medicago polymorpha and target rhizobium LL2 in a concentration range of 500-1000 muL / L ‑1 of eugenol, can significantly reshape rhizosphere microbial community structure by selectively inhibiting competitive microorganisms such as bacillus and streptomyces in soil, and is environment-friendly and free of antibiotic residue risk, thereby providing a green and efficient new approach for improving symbiotic nitrogen fixation efficiency of legume-rhizobium.

The present invention provides a method and a composition for increasing nitrogen fixation of at least one strain of a nitrogen fixing bacteria or acquisition of nitrogen for a plant in need thereof.

The invention relates to an artificial phosphate-solubilizing module and application thereof in improving the nitrogen-fixing and phosphate-solubilizing capabilities of nitrogen-fixing microorganisms. The artificial phosphate solubilizing module sequentially comprises an inducible promoter element and a conservative acid phosphatase gene acpA from a pseudomonas fluorescens strain from upstream to downstream. The artificial phosphate solubilizing module is inserted into a preset target site of a host microorganism chromosome in a homologous recombination manner, so that a host has the characteristic of efficiently expressing acid phosphatase, and the organic phosphorus degradation capacity and nitrogen fixation capacity of the host are remarkably improved. The method has high application value in the field of preparation of high-efficiency microbial fertilizer products. The method opens up a new path for agricultural green sustainable development, and has extremely important scientific significance and wide application prospects.

The invention discloses a tank type fermentation process capable of realizing collaborative emission reduction of NH3, N2O and CH4. The tank type fermentation process comprises the following steps: S1, pretreating raw materials; s2, material introduction; s3, supplementing materials; and S4, continuous fermentation. The process has the advantages of obvious environmental benefit, realization of collaborative emission reduction of greenhouse gas and odor, final reduction of the compost load, high resource utilization efficiency, realization of closed-loop circulation of water and fertilizer, reuse of nutrient-rich leachate as a nitrogen fixation carrier, realization of zero liquid discharge and nutrient in-situ recovery, increase of the total nutrient content of a compost product, and realization of environmental protection and environmental protection. The nitrogen loss is greatly reduced, and beneficial components such as zeolite and pyroligneous liquor are added, so that the content of organic matters and nutrients of the finished compost is high, the water and fertilizer retention capacity is high, essential elements for promoting plant growth are contained, and the commodity value is remarkably improved.

The invention relates to the technical field of microorganisms, in particular to bacillus aryabhattai OF326 and application of the bacillus aryabhattai OF326 to preparation of bio-organic fertilizer and disease resistance, the bacillus aryabhattai OF326 is a probiotic screened by the applicant, research finds that the strain has the functions of IAA production, phosphorus solubilization and nitrogen fixation, growth of rice seedlings can be remarkably promoted, and the yield of the rice seedlings is increased. The method is suitable for secondary fermentation of organic fertilizers with sheep manure and traditional Chinese medicine residues as raw materials, and the viable count of beneficial bacteria is increased. In addition, tests show that the strain OF326 has a remarkable antagonistic effect on ralstonia solanacearum, and a clear antibacterial zone is formed; and by compounding with inositol or trehalose-6-phosphoric acid (T6P), the proliferation of the strain can be further promoted, and the growth rate of the strain is increased. In conclusion, the strain still keeps good growth-promoting and disease-resistant functions under the waterflooding condition, and is suitable for development of biological fertilizers and disease-resistant preparations.

The application relates to application of Bi2O2Se in a photocatalytic nitrogen fixation reaction and belongs to the technical field of photocatalytic nitrogen reduction. The BOS catalyst with intrinsic weak hole oxidation capacity realizes efficient and stable photocatalytic ammonia synthesis without a sacrificial agent. The BOS has an optimized narrow band gap structure and a unique straddling energy band arrangement, the valence band position is shallow, the hole oxidation capacity is weak, the oxidation degradation of NH4 + Can be effectively inhibited, and the conduction band position is suitable, so that the thermodynamic driving force of nitrogen reduction is ensured. The catalyst has excellent light absorption capacity in a wide spectral range (extending to the near-infrared region), and the apparent quantum efficiency reaches 0.12% at a wavelength of 700 nm. The ammonia synthesis rate of the BOS without a sacrificial agent reaches 61 muM.h ‑1 -1, which is 8.3 times and 2.8 times that of carbon nitride and cadmium sulfide, and the catalyst maintains high activity in an air atmosphere, has excellent oxygen compatibility and cycle stability.

The invention relates to a complex microbial inoculant for mixed compost of sugarcane filter mud and sugarcane leaves and an application method, and belongs to the technical field of microorganisms, the complex microbial inoculant comprises a high-temperature microbial inoculant, and the high-temperature microbial inoculant is prepared from thermophilic carbon monoxide streptomyces G-4-7, brevibacillus brevis G-5-2 and thermophilic chaetomium; the medium-temperature microbial inoculum is prepared from trichoderma longibrachiatum, staphylococcus warneri LH-403 and bacillus thuringiensis LMU-81, and the medium-temperature microbial inoculum is prepared from the trichoderma longibrachiatum and the staphylococcus warneri LH-403; the complex microbial inoculant adopts a two-stage inoculation method. The complex microbial inoculant can effectively enhance degradation of lignocellulose so as to promote compost maturity, the humification degree of compost is further improved through the biological enhancement effect, the nitrogen fixation effect of the compost is facilitated, the germination index of the compost is improved, resource utilization of organic matter rich in lignocellulose can be promoted, and the environment-friendly effect is achieved. And a technical basis is provided for a green treatment mode of agricultural wastes.

This invention discloses a salt-tolerant Cladosporium 550W1 strain and its application in promoting nodulation and nitrogen fixation in leguminous plants, belonging to the field of microbial technology. The salt-tolerant Cladosporium (… Cladosporium halotolerans Cladosporium 550W1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 41943, provides a salt-tolerant Cladosporium 550W1 that can significantly promote nodulation and nitrogen fixation in legumes, increase plant biomass and nitrogen accumulation, and reduce dependence on chemical nitrogen fertilizers. It has broad application prospects and environmental and ecological benefits.