663 results about "Nitrogen fixation" patented technology

Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in glnD, wherein said modification is selected from the group consisting of: deletion of the entire gene, deletion of substantially the entire gene, deletion of an ACT domain, deletion of more than 50% of an ACT domain, deactivation of an ACT domain, and deactivation of an UTase domain.

Systems for plant culture include a chamber featuring one or more walls enclosing a spatial volume internal to the chamber, where the one or more walls include a surface for supporting a plant within the enclosed spatial volume, a gas delivery apparatus with at least one gas source, a nutrient delivery apparatus with a reservoir, a sampling apparatus connected to a port formed in the one or more walls, and a controller configured so that during operation of the system, the controller activates the nutrient delivery apparatus to deliver an aqueous growth medium to the plant, and activates the gas delivery apparatus to deliver into the enclosed spatial volume a mixture of isotopically-substituted gases. Also provided are methods of use of the system for measuring nitrogen in a plant and for identifying microbes capable of providing fixed nitrogen to a plant.

The invention provides a strain of Burkholderia graminicola as well as a bacterial agent and application of the Burkholderia graminicola. The preservation number of the Burkholderia graminicola is CCTCC (China Center For Type Culture Collection) NO: M20242399. The Burkholderia paragallinarum provided by the invention has the functions of nitrogen fixation, phosphate solubilization, siderophore production and indoleacetic acid production. The Burkholderia grassy and the fungicide taking the Burkholderia grassy as the effective active component can promote the germination of plant seeds and the growth of crops.

The invention discloses bacillus cereus and application thereof, and belongs to the technical field of microorganisms. The bacillus cereus SNF7 is separated from a saline-alkali soil sample, has the functions of resisting salt, fixing nitrogen and promoting growth, has an obvious inhibition effect on some common soil-borne diseases in agriculture, such as pathogenic bacteria of peanut fruit rot, tomato root rot and the like, has multiple functions of resisting salt, fixing nitrogen, biologically preventing and controlling and the like, and has a wide application prospect. The method can be widely applied to crop planting in large saline-alkali soil in China.

The invention relates to the technical field of agricultural biology, and discloses an auxiliary cultivation method for promoting sugarcane biological nitrogen fixation, which comprises the following steps: (1) breeding a sugarcane variety with high nitrogen-fixing bacteria affinity, and preparing an environmental stress resistant nitrogen-fixing bacteria agent; (2) carrying out acidification improvement and carbon source pre-burying on the planting soil; (3) inoculating the nitrogen-fixing bacterial agent to the sugarcane seed stem or rhizosphere area; (4) maintaining the metabolic activity of nitrogen-fixing bacteria by dynamically regulating and controlling rhizosphere carbon source supply and oxygen partial pressure; (5) adjusting a fungicide supplementary application strategy in real time based on the environmental data; and (6) solidifying the symbiotic character through intergenerational epigenetic treatment. Through host-microorganism coevolution and intelligent dynamic regulation and control, the nitrogen fixation efficiency and environmental adaptability of the sugarcane-nitrogen-fixing bacteria symbiotic system are remarkably improved; and epigenetic and flora iteration technologies are combined, so that stable transmission of cross-generation characters is realized, and a low-consumption and high-benefit ecological solution is provided for sustainable agriculture.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention provides a saline-alkaline tolerant Bachu halomonas strain, which is characterized in that the saline-alkaline tolerant Bachu halomonas strain is named as halomonas sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.28831, and the preservation date is November 01, 2023; the nucleotide sequence of the 16S rRNA is as shown in SEQ ID NO: 1. The invention further provides application of the strain, and the strain is used for improving saline-alkaline resistance of plants, reducing soil salinity and alkalinity and promoting plant growth and is used for preparing fertilizer with plant growth promoting and saline-alkali soil improving effects. The strain disclosed by the invention has the functions of resisting salt and alkali, reducing salt and alkali, fixing nitrogen, decomposing organic phosphorus, dissolving inorganic phosphorus, producing IAA, producing oxidase, producing catalase, producing volatile acidic substances and promoting plant growth. The strain obtained by the invention has important application values in the aspects of improving saline-alkali stress resistance of crops, improving physicochemical properties of saline-alkali soil, increasing crop yield and the like, can be prepared into a fertilizer with plant growth promoting and saline-alkali soil improving effects for use, is a great innovation in saline-alkali soil improvement and crop planting, and has a wide application prospect.

The invention belongs to the technical field of microorganisms, and provides a growth-promoting P.adami M6 and application thereof, the P.adami M6 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M20242835, the preservation date is December 18, 2024, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, and the preservation number is CCTCC No: M20242835. The strain is separated from rhizosphere soil of field planting foxtail millet in the Inner Mongolia red peak area, and is obtained through artificial separation and purification; the strain has the growth promoting characteristics of nitrogen fixation, phosphorus dissolution, IAA production and siderophore production; the M6 fungicide is used for inoculating millet, so that the growth of millet seedlings can be obviously promoted, and the plant height, the root length and the overground part and underground part biomass of the millet are obviously increased; m6 is used as a microbial agent to soak seeds during sowing and irrigate a bacterial liquid once 7 days after sowing, so that the growth effect of millet is best.

Burkholderia, and a composition, use and usage method thereof. Provided are the Burkholderia, and a composition comprising the strain and / or a metabolite, culture, fermentation broth and / or extract thereof. The Burkholderia or the composition thereof can generate a siderophore, induce plants or seeds thereof to generate systemic resistance to plant pathogens, and reduce the chemotaxis of plant pathogens to plants, so that prevention and treatment of plant diseases and insect pests are achieved. In addition, plant root system development can be promoted, a nitrogen fixation effect is provided, a phosphate solubilization effect is provided, indoleacetic acid is generated, and weeds are removed, thereby promoting plant growth.

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention belongs to the technical field of biological agents, and particularly relates to bacillus siamensis DG-39, a microbial agent and application of the bacillus siamensis DG-39 to inhibition of early bolting of angelica sinensis. According to the bacillus siamensis DG-39 and the microbial inoculum containing the bacillus siamensis DG-39, the physiological and biochemical states of angelica sinensis are effectively changed mainly by increasing the content of endogenous melatonin of angelica sinensis, reducing the content of abscisic acid, auxin and gibberellin and promoting circulation and utilization of nitrogen, and the photoperiod of the angelica sinensis can be effectively improved by reducing expression of related genes of the photoperiod. The effect of inhibiting early bolting of the angelica sinensis is achieved. Meanwhile, the bacillus siamensis DG-39 microbial inoculum has the characteristics of fixing nitrogen, not dissolving phosphorus and producing siderophore, and can promote the absorption and utilization of plants on nitrogen, so that the promotion effect of excessive phosphorus on bolting is avoided; meanwhile, the bacillus siamensis DG-39 laccase is high in activity, can remove phenolamine substances for promoting bolting, and is beneficial to reducing the bolting rate.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plants life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

The invention discloses a salt-tolerant growth-promoting bacillus cereus strain and an application thereof. In particular discloses bacillus cereus T146, and the preservation number of the bacillus cereus T146 is CGMCC (China General Microbiological Culture Collection Center) No.30429. The bacillus cereus T146 disclosed by the invention has good biofilm production, siderophore secretion and nitrogen fixation capabilities, and can remarkably increase the root length, the overground dry weight, the plant height, the number of lateral roots and the average density of the lateral roots of wheat under the condition of salt stress. According to the invention, a low-cost, efficient and applicable culture medium aiming at the bacillus cereus T146 is determined, a stable and controllable fermentation system is established, the preparation method of the microbial inoculum with high mature spore number is obtained by optimizing a spray drying process, and the active spore number of the microbial inoculum can reach 1.3 * 10 < 11 > CFU / g. The bacillus cereus T146 has good development and application prospects in the fields of microbial agents, microbial fertilizers, soil improvement and the like.

The invention belongs to the technical field of rhizosphere microorganisms, and provides a strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens and application of the strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens in growth promoting and repairing of saline-alkali soil. The strain not only grows fast, but also has growth promoting functions such as nitrogen fixation, phosphorus solubilization and potassium solubilization. The strain is preserved in the China General Microbiological Culture Collection Center on March 11, 2025, called CGMCC for short, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the biological preservation number of the strain is CGMCC NO.33793. The SP-38 strain has multiple growth promoting functions, and through salt stress water culture tests and in-situ saline-alkali soil pot tests of corn, the strain has good effects in the aspects of improving the crop yield and repairing saline-alkali soil.

The invention relates to the technical field of microorganisms, in particular to application of bacillus glucans and a complex microbial inoculant thereof in preparation of bio-organic fertilizers, the strain Mh85 is separated from the root system of apple rootstock Malus hupehensis treated by a research group through trichoderma TB2, has good IAA production, nitrogen fixation, inorganic phosphorus decomposition and siderophore production capacities, and can be used for preparing bio-organic fertilizers. The strain Mh85 not only can directly promote plant growth, but also has a good growth-promoting effect on trichoderma TB2, and when the strain is compounded with the trichoderma TB2 to form a complex microbial inoculant, tomato seedling pot experiments are carried out. The complex microbial inoculant shows a synergistic effect, effectively overcomes the problems of unstable growth promoting effect, insufficient synergistic effect among strains and the like of an existing single microbial preparation, provides an efficient and stable technical scheme for improving plant growth, improving root development and increasing crop yield, and is a good raw material for preparing a bio-organic fertilizer.

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

The invention discloses a Brucella with antibacterial activity and application of the Brucella. The invention discloses Burkholderia sp.HNIV0701, and the preservation number of the Burkholderia sp.HNIV0701 is GDMCC No: 66007. The Burkholderia sp.HNIV0701 disclosed by the invention has a stable, efficient and broad-spectrum antibacterial property, has an inhibition effect on a plurality of plant pathogenic bacteria such as Botryosphaeria dothidea, Phytophthora nicotianae, Fusarium solani, Alternaria alternata, Thanatepora cucurbitae, Ralstonia solanacearum and the like, and also can be used for inhibiting Staphylococcus aureus and / or Listeria monocytogenes, so that the Burkholderia sp.HNIV0701 can be used for inhibiting a plurality of plant pathogenic bacteria such as the Botryosphaeria dothidea, the Phytophthora nicotianae, the Fusarium solanum, the Alternaria solanacearum and the like. The Brucella HNIV0701 can hydrolyze organic phosphorus and can be used for soil improvement. The Brucella HNIV0701 has nitrogen fixation capability and has the potential of promoting plant growth.

Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

The invention discloses a regulation and control method for recarburization and nitrogen fixation of newly-reclaimed desertification saline-alkali soil, which is characterized in that the soil microenvironment is improved by combined application of bio-organic fertilizer / charcoal and attapulgite fertilizer, and nitrogen fixation of soil in a growth period and improvement of a soil structure are realized by mixed sowing of gramineous green manure crops and leguminous green manure crops. And after the growth period ends, returning the green manure to the field to realize soil recarburization. According to the recarburization and nitrogen fixation regulation and control method for the newly-reclaimed desertification saline-alkali wasteland, the soil nutrient and carbon reserve can be remarkably improved, and compared with other green manure crops or combinations, the total nitrogen and organic carbon of the soil are remarkably improved after the method is implemented. Therefore, the method has important significance on recarburization, nitrogen fixation and fertilization regulation and control of newly-reclaimed desertification saline-alkali soil, and can provide a development idea for efficient development and utilization of saline-alkali sand and guarantee of agricultural production safety.

The invention relates to the technical field of environmental microorganisms, in particular to arthrobacterium Arth.R8 and application thereof, and the preservation number of the arthrobacterium Arth.R8 is GDMCC No.63567. The arthrobacter Arth.R8 has the effects of fixing nitrogen, dissolving organic phosphorus, promoting growth and promoting nodulation. The arthrobacter Arth.R8 disclosed by the invention can be used for promoting absorption and utilization of nutrient substances such as nitrogen and phosphorus in a plant growth process, inhibiting growth of fusarium graminearum and promoting plant growth, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention discloses plant probiotics with water retention, growth promotion and disease resistance effects and application thereof. The invention provides a complex microbial inoculant, which is prepared from the following active ingredients: 1) bacillus tequilensis Bac365 (GDMCC No: 65241), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) Enterococcus querebrasis Ent274 (GDMCC No: 65242) or Pseudomonas plecoglossa Pse350 (GDMCC No: 65243), and the active ingredients of the complex microbial inoculant are as follows: 1) bacillus tequilensis Bac365 (GDMCC No: 65243), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) the active ingredients of the complex microbial inoculant. The strain provided by the invention has good nitrogen fixation, phosphorus dissolution, IAA production, HA production and disease resistance, can effectively increase the utilization efficiency of water in drought soil, significantly promote plant growth and increase crop yield, and has good application prospects in the aspects of water retention and sand stabilization, agricultural production and the like.

The invention relates to a method for improving soil fertility and enzyme activity of a rice field by using a nitrogen-fixing cyanobacteria biological membrane, which comprises the following steps: step 1, expanding propagation of nitrogen-fixing cyanobacteria: selecting anabaena globifera as a rice field application algae species, and performing expanding culture on the algae species to obtain a nitrogen-fixing cyanobacteria secondary algae species; step 2, applying nitrogen-fixing cyanobacteria: applying the nitrogen-fixing cyanobacteria in a rice base fertilizer period and a tillering period, and replacing part of chemical fertilizer with the nitrogen-fixing cyanobacteria to reduce the application amount of the chemical fertilizer; secondly, the nitrogen-fixing cyanobacteria returns to the rice field through biological nitrogen fixation and rotting decomposition of dead algae, the content of nitrogen and carbon in the soil is increased, the soil fertility is improved, and the nitrogen-fixing cyanobacteria can provide nutrient substances for microorganisms in the soil, improve the enzyme activity of the soil and improve the quality of the soil when applied to the rice field.

The invention relates to the technical field of environmental microorganisms, in particular to Chryseobacterium Chry.R1 and application of the Chryseobacterium Chry.R1, and the preservation number of the Chryseobacterium Chry.R1 is GDMCC (China General Microbiological Culture Collection Center) No.63568. The Chryseobacterium Chry.R1 has the effects of fixing nitrogen, inhibiting fusarium graminearum and promoting plant growth. The Chryseobacterium Chry.R1 disclosed by the invention can be used for promoting the absorption and utilization of nitrogen elements in a plant growth process, inhibiting the growth of fusarium graminearum and promoting the growth of plants, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention aims to provide the aluminum ash-based chemical-looping denitrification ammonia synthesis method, active aluminum components, catalytic components and primary structures in the aluminum ash are fully utilized as nitrogen carrier raw materials, and aluminum ash denitrification and continuous ammonia synthesis are realized through ammonia release and nitrogen carrying respectively. The method specifically comprises the following steps: (1) grinding the aluminum ash to a required particle size range; (2) high-temperature water vapor is introduced into the aluminum ash which is placed in the ammonia release reactor and ground in the step (1), and primary ammonia release is carried out; (3) uniformly mixing the ammonia release reaction product obtained in the step (2) with a carbon source in proportion, introducing nitrogen into a nitrogen-carrying reactor, and realizing nitrogen fixation regeneration through carbon thermal reduction; (4) introducing an oxidizing agent into the product subjected to nitrogen fixation in the step (3) in a carbon removal reactor for carbon removal; and (5) conveying the nitrogen carrier decarbonized in the step (4) back into the ammonia release reactor, and repeating the steps (2), (3) and (4) to realize the aluminum ash-based chemical-looping ammonia synthesis circulation. Compared with the prior art, based on the aluminum ash, through the properties of a chemical looping technology, normal pressure, low consumption and elimination of aluminum ash harm, preparation of synthesis ammonia coupling synthesis gas is achieved, and a new way is provided for energy reform and development of a synthesis ammonia new technology and mining of aluminum ash recycling and energy regeneration.

The invention belongs to the technical field of microorganisms, and relates to a sphingomonas sp. Strain with a soil reclamation fertilizing effect, which is preserved in China General Microbiological Culture Collection Center on October 17, 2024 with a preservation number of CGMCC No.32244. The strain has the capabilities of nitrogen fixation, phosphate solubilization, potassium solubilization and growth promotion, can also significantly promote straw decomposition and nutrient release, and has the advantages of high efficiency, high yield, high yield and the like. When being used in reclamation soil in a coal mine area, the fertilizer can improve the crop yield, effectively and remarkably increase the number of soil microorganisms, change the community structure of the soil microorganisms, improve the diversity of the soil microorganisms and accelerate the recovery of soil microbial communities, and can be widely applied to improvement of the fertility of the reclamation soil in the coal mine area.

The invention belongs to the technical field of microorganisms, and particularly relates to halophilous bacillus, a microbial agent and application of the halophilous bacillus. The invention provides bacillus halophilous andaensis, and the preservation number of the bacillus halophilous andaensis is CCTCC (China Center for Type Culture Collection) M 20232394. The invention further provides a microbial inoculum which comprises the halophilous bacillus. The invention further provides application of the halophilous bacillus or the microbial inoculum to promotion of plant growth under saline-alkali stress, improvement of plant stress resistance, soil improvement and crop production. The halophilous bacillus provided by the invention has excellent saline-alkaline tolerance, can only grow under saline-alkaline conditions, also has the growth promoting characteristics of reducing salt, producing acid, reducing alkali, fixing nitrogen and the like, and can relieve the salt damage phenomenon and improve the crop yield and quality.

The invention provides a microorganism synergistic nitrogen fixation fertilizer based on flavone induction and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The fertilizer induces the formation of an azotobacter biofilm through a flavonoid compound, promotes the colonization and nitrogen fixation of rhizosphere azotobacter, improves the nitrogen supply capability of soil, and reduces the dependence on chemical nitrogen fertilizer. The fertilizer comprises the following components: 5-15% of a flavone extract, 3-8% of an azotobacter, 20-30% of an organic carrier, 30-40% of an inorganic nutrient medium and 5-15% of a filler. The preparation method comprises the following steps: mixing the organic carrier and the inorganic nutrient substrate, adding the filling agent, granulating, adding the nitrogen-fixing bacterial agent and the flavone extract to form a composite liquid, spraying the composite liquid, drying and packaging. Compared with the prior art, the fertilizer disclosed by the invention has the advantages of remarkably improving the nitrogen fixation efficiency, reducing the use of chemical nitrogen fertilizer, enhancing the slow release performance, improving the fertilizer utilization rate, improving the soil ecological environment and promoting the healthy growth of plants. The method is simple, efficient and suitable for large-scale production and application.

The invention discloses bacillus for preventing and treating anthracnose of camellia oleifera and application of the bacillus. The preservation number of the bacillus CH-1-7 provided by the invention is GDMCC (Glucose Digital Microorganism Center) No. 66219; the bacillus amyloliquefaciens has the capability of generating living-promoting substances, can generate organic phosphorus and siderophores, has the nitrogen fixation capability, can inhibit the growth of various pathogenic bacteria, especially colletotrichum gloeosporioides, can be used for preventing and treating camellia oleifera anthracnose, and has important application value in the field of plant protection.

The invention belongs to the technical field of microorganisms, and relates to a strain of herbaspirillum as well as a fermentation method and application thereof. The invention provides a strain of brassica juncea, the brassica juncea is brassica juncea YLHb25.7, the brassica juncea is named as Herbaspiril huttiense, the brassica juncea is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35297. The invention also provides a preparation method of the brassica juncea. The herbispirillum sp. YLHb25.7 disclosed by the invention has the capabilities of producing ACC deaminase, producing IAA and fixing nitrogen, and has good drought resistance and saline-alkaline tolerance. The herbaspirillum sp. YLHb25.7 and the fermentation product thereof can promote the growth of wheat and corn, can be used as a microbial agent or bacterial fertilizer, and can be applied to the agricultural market.

The invention relates to an application of a nano silicon dioxide combined flora in relieving plant salt stress, which comprises the following steps: applying nano silicon dioxide to soil in which soybeans are to be planted, then planting the soybeans in the soil, and adding a simplified salt-tolerant growth-promoting synthetic flora microbial inoculum; wherein the synthetic flora is separated from saline-alkali soil, the growth promoting functions of nitrogen fixation, phosphorus dissolving, potassium dissolving and the like of the synthetic flora are measured, and the growth promoting effect of a single bacterium is verified through a pot experiment. A simplified flora SynC1 is constructed, and the relieving effect of combined application of the nano silicon dioxide and the flora on soybean salt stress is evaluated through a pot experiment. It is found that combined application of nano-silica and SynC1 can better promote root development, increase the proline content and promote soybean growth under salt stress, and has excellent application potential in agricultural soybean production.