912 results about "Nitrogen fixation" patented technology

The invention discloses a production method for a biological organic fertilizer which belongs to the field of manufacturing of composite fertilizers. The production method comprises the following steps: with pig manure, cow dung, chicken manure, sheep manure, vinasse, vinegar residue, cassava residue, furfural residue, uncontaminated river mud, corn straw, peanut straw, rape straw, a cottonseed cake, plant ash, a deodorant, a biological nitrogen fixation bacterium, a phosphate solubilizing bacterium, a potassium solubilizing bacterium, a nodule bacterium and a phosphorus activator used as raw materials, carrying out tank continuous fermentation to allow the raw materials become thoroughly decomposed; carrying out deodorizing and removing water; and carrying out granulation to obtain the granular biological organic fertilizer. The fertilizer has the following advantages: a complete range of nutrient elements, capacity of improving physical and chemical properties of soil and enhancing water retention, fertilizer retention and fertility supplying capacity of the soil, no corrosion and damage to roots and seedlings and capacity of substantially improving the quality of an agricultural product.

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

The invention relates to a heavy metal lead and cadmium antibiotic-resistant bacterium and a method for strengthening lead and cadmium heavy metals in the vegetable extraction soil, and belongs to the agricultural and environmental pollution treatment technical field. The J62 culture stain is appraised as the Burkholderia sp, has antibiotic resistance to a plurality of heavy metals, in particular to lead and cadmium, respectively reaching Pb<2+>1000mg/L and Cd<2+>2000mg/L. The invention has the characteristics of nitrogen fixation and phosphate solubilizing, and can promote the growth of the plant and improve the stress resistance of the plant. The effective living microbial numbers of the liquid preparation are above a billion per millimeter, and the effective living microbial numbers of the solid preparation are 200 millions per gram. When the seeds of the plant are seminated in humid soil containing heavy metals, 10 to 20mL of the bacteria liquid of 10<8> bacteria J62/mLs is inoculated in each grams of soil once or in two times. The invention promotes the growth of the plant, strengthens the enrichment of heavy metal lead and cadmium of the plant, and improves the extraction and recovery efficiency of the plant.

The invention relates to the field of environmental protection and discloses a soil conditioner, as well as a preparation method and application thereof. The soil conditioner comprises raw materials in parts by weight: 50 to 60 parts of wood fibers, 14 to 18 parts of biochar, 25 to 30 parts of humus, 3 to 6 parts of polysaccharide, 1 to 3 parts of seaweed essence, 0.001 to 0.005 part of Trichoderma and 0.001 to 0.005 part of nitrogen fixation bacteria. The invention also discloses a preparation method, the application and a using method of the soil conditioner, the soil conditioner has the advantages of improving soil physical and chemical properties and promoting the turf forming performance of plants, and the preparation and application methods are simple and convenient, so that the popularization and application are facilitated.

The invention discloses bacillus megaterium and an application thereof, and belongs to the field of microorganisms. A plant growth promoting bacterium (bacillus megaterium JX15) is collected in the China General Microbiological Culture Collection Center on Dec.20, 2011, the class name is bacillus megaterium and the collection number is CGMCC No.5622. The bacillus megaterium has the beneficial effects that a strain can produce indoleacetic acid with high yield, can be used for nitrogen fixation and can grow by using sparingly-soluble phosphates as phosphorous sources, so as to improve the utilization rates of fertilizers, promote plant root development and absorption of the fertilizers, and increases the contents of soil mineral nitrogen and available phosphorus; the strain has a good effect on promoting growth of peanuts; the high-yield indoleacetic acid promotes the growth and the development of the peanuts; and the nitrogen and phosphate fertilizer utilization rates of the peanuts are relatively high due to the increasing of the contents of the soil mineral nitrogen and the available phosphorus.

The invention discloses a novel strain CLIMAX-VSF of streptomyces fradiae. The preservation number of the novel strain is CGMCC No. 5133; the preservation date is August 12, 2011; and the preservation department is China General Microbiological Culture Collection Center. The invention further discloses a microbial inoculum containing the novel strain. The novel strain CLIMAX-VSF of the streptomyces fradiae, which is provided by the invention, has quite obvious performances of disease resistance, nitrogen fixation, phosphate solubilization and somatotropin secretion. By using the strain, the germination rate and the growth speed in a seedling stage can be obviously improved under the condition that nutrients are not added to soil, and furthermore, diseases in the seedling stage and a growth period are obviously inhibited. On the aspect that the strain is used for the mixed preparation and the synergy of a fertilizer, the strain shows to have the favorable effect of activating soil nutrients and the performance of resisting a plurality of cultivation obstacles.

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared by mixing the following raw materials: yeast fermentation powder residues, an organic fertilizer, urea, K2SO4, NH4H2PO4, clay and a compound microbial agent; and the compound microbial agent is formed by fermentation of bacillus mucilaginosus, bacillus megaterium, bacillus cereus, bacillus thuringiensis, azotobacter chroococcum, pichia farinose and streptomyces microflavus. The compound microbial fertilizer disclosed by the invention has a variety of microbes with the effects of nitrogen fixation, phosphorus dissolution and potassium dissolution, can increase the nitrogen content in soil, improves the utilization rate of the chemical fertilizer, and is favorable for improving the structure of the soil and improving the yield and quality of crops; and simultaneously, the microbes in the fertilizer can produce a variety of metabolites, and the metabolites can effectively resist diseases and insect pests.

This invention discloses a method for preparing microbial compound fertilizer chelated with trace elements for spray and drop irrigation. The method comprises: hydrolyzing raw material of protein wastes to obtain composite amino acids, adding trace elements to chelate composite amino acids, and combining with beneficial bacteria (azotobacter, phosphorus fertilizer bacteria, potassium bacteria, bacillus, etc.) to obtain the microbial compound fertilizer. The microbial compound fertilizer has obvious nitrogen fixation, phosphorus and potassium leaching, and growth promotion functions, and has high utility. The release speed is controllable, and the microbial compound fertilizer can improve and activate soil, and improve crop quality.

The invention relates to a bacillus megatherium SJ-7 strain and an application thereof, and belongs to the technical field of organisms. Strain separation, purification and cultivation are carried out from the natural world by a common method, so as to obtain the bacillus megatherium (bacillus megatherium) SJ-7 strain; the bacillus megatherium SJ-7 strain is reserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms; the reservation number is CGMCC No.7169; and the bacillus megatherium SJ-7 strain has strong effects of solubilizing phosphate and potassium and fixing nitrogen, and has good antagonism on a plurality of disease funguses, and can be applied to preparation of a drug for preventing and treating plant fungi diseases. Compared with the reported other bacillus megatherium, the bacillus megatherium SJ-7 disclosed by the invention has the advantages that the bacillus megatherium SJ-7 has good effects of solubilizing potassium, degrading refractory inorganic phosphorus in soil, and fixing nitrogen, can promote plant growth, and has strong inhibitory action on a plurality of plant pathogenic fungi of causing a gibberellic disease, a cotton drooping disease, wheat sharp eyespot and botrytis.

This invention relates to new consolidation sand method with terraneous nitrogen fixation blue-green algae, which is completed by the following steps: blue-green algae foster, inoculation environment, inoculation time and inoculation quantity, and it uses the microorganism technology such as terraneous nitrogen fixation blue-green algae to achieve the fast consolidation of the flowing sand in the fixed and the half fixed desert area and achieve the ecology rehab in the engineering trouble area of the fixed and half fixed desert area. The applied nitrogen fixation blue-green algae can do photosynthesis by itself by the limited precipitation in desert, it grows up, and produces hull, which achieves the purpose of adding organic matter in soil and improving the structure of the soil and preventing and consolidating sands.

The invention relates to a water purifying agent for pond culture. The water purifying agent is powdery mixture which is prepared by uniformly mixing the following components in part by weight: 100 to 200 parts of bacillus subtilis, 100 to 200 parts of nitrobacteria, 150 to 200 parts of photosynthetic bacteria and 400 to 650 parts of zeolite powder, wherein the water purifying agent preferably comprises 100 parts of bacillus subtilis, 100 parts of nitrobacteria, 150 parts of photosynthetic bacteria and 650 parts of zeolite powder; the bacillus subtilis is light yellow powder and the total count of viable bacteria is more than or equal to 200 hundred million per one gram; the nitrobacteria is light grey powder and the total count of the viable bacteria is more than or equal to 80 hundred million per one gram; the photosynthetic bacteria is grey white powder and the total count of the viable bacteria is more than or equal to 30 to 50 hundred million per one gram; and the zeolite powder is clinoptilolite with particle size of 180 meshes. The water purifying agent has the advantages of improving water quality, conducting the function of oxidation, ammoniation, denitrification, dephosphorization, sulfurization, nitrogen fixation and the like, constructing and maintaining good ecological water environment, preventing and treating diseases, enhancing the anti-disease capacity of animals bred in the pond and improving the survival rate. The water purifying agent has a reasonable formula, is simple to prepare and has obvious application effect.

The invention relates to a biological carbon-based ecological fertilizer. The biological carbon-based ecological fertilizer is prepared from, by weight, 30 to 40 parts of biological carbon, 10 to 30 parts of humic acid, 10 to 20 parts of urea, 5 to 15 parts of ammonium dihydrogen phosphate, 5 to 15 parts of potassium sulphate, 1 to 5 parts of pyroligneous liquid, and 0.5 to 2 parts of trace element. The biological carbon-based ecological fertilizer is capable of increasing soil organic matter content and soil cation exchange capacity effectively, activating soil, promoting formation of soil aggregate structures, preventing and treating soil salinization, avoiding successive cropping obstacles, improving nitrogen fixation and phosphate solubilizing functions, releasing phosphorus, potassium, and trace element immobilized in soil, improving soil fertilizer, water, and soil moisture conservation capacity, reducing fertilizer application amount, reducing grain pollution caused by soil with excess heavy metal, stimulating crop growth, and improving agricultural product quality obviously.

The invention relates to a fertilizer. In order to reduce the fertilizer loss and increase the utilization ratio of nutrients in the fertilizer, the invention provides an organic and inorganic compound fertilizer containing organic mineral and inorganic mineral. A preparation method of the compound fertilizer comprises the following steps of: uniformly mixing 17-18% of kieselguhr mineral powder, 17-18% of humic acid mineral powder and 23-24% of organic fertilizer, then adding 0.5% of microbial compound agent for nitrogen fixation, phosphorus releasing and potassium releasing, and fermenting to prepare a biological organic fertilizer; uniformly mixing the biological organic fertilizer with 30% of N, P and K inorganic fertilizer, then adding 9-10% of gypsum powder as a binding agent, granulating by squeezing, sieving, and drying in the air to obtain the organic and inorganic compound fertilizer D. Secondary and trace elements accounting for 0.5-2.5% of organic and inorganic compound fertilizer D by weight are added before the gypsum powder is added. The organic and inorganic compound fertilizer can increase the crop yield by more than 15-25%, improve the utilization ratio of the fertilizer to more than 60%, realize the only one application time of the base fertilizer for first crop, and reduce the labor intensity and the fertilizer application cost.

The invention discloses a growth nutrient solution special for grapes and a production process thereof. The growth nutrient solution special for grapes contains an early stage nutrient solution, a middle stage nutrient solution and a later stage nutrient solution. The early stage compoents contain bean cake solution, urea, microelements, fulvic acid, amino acid solution, borax, phosphoric acid, amino acid potassium, amino acid zinc, vitacat and biogas slurry. The middle stage components include bean cake solution, phosphoric acid, fulvic acid potassium, amino acid magnesium, amino acid calcium, borax, amino acid zinc, Sophora alopecuroide tender juice, pyroligneous juice, sodium bicarbonate, microelements and biogas slurry. The later stage components contain bean cake solution, urea, phosphoric acid, fulvic acid potassium, amino acid calcium, Sophora alopecuroide tender juice, pyroligneous juice, vitacat, microelements and biogas slurry. The production process of the production process includes 1 raw material preparing and 2 solution preparing. Contents of organic nitrogen, phosphorus and potassium are increased, parasite expelling and bacteriocidal substances are added, and therefore pest and disease damage can be prevented. Beneficial biological bacteria are added and have nitrogen fixation, phosphorus diluting and potassium dissolving effects. The growth nutrient solution can improve grape yield by 0-15% and sugar content by 2-4%.

The invention discloses biologically organic fertilizer and thereof preparation method thereof. The biologically organic fertilizer is made by secondarily biologically fermenting the mixture which consists of castor-oil plant meal shell, grass carbon, castor-oil plant meal and stalk powder with the weight rate of 40 to 60:10 to 20:5 to 15:20 to 30 through composite microbial inoculums which are 0.02 percent of the mixture and composite microbial inoculums which are 0.02 percent of the mixture, and by mixing with phosphor releasing, potassium releasing and nitrogen fixation target bacterial flora. The N, the P, the K nutrients of the biologically organic fertilizer provided by the invention are increased by 30 to 40 percent than that of the commercial biologically organic fertilizer, the organic matters are increased by 40 to 50 percent and the beneficial microorganism is increased to more than 0.02 hundred millions per gram. Besides, the production period of the invention is shortened by 10 to 20 days than that of the common organic fertilizer with simple preparation method. Moreover, the yield can be increased by 10 to 20 percent when the invention is applied on crops such as vegetables and fruit trees.

The invention discloses nutritional soil for plant cultivation of a karst landform. The nutritional soil is used for solving the problem that the existing soil of karst-landform regions is not conducive to plant growth. The nutritional soil disclosed by the invention comprises foreign soil, an organic fertilizer, acidic soil microflora, organism decomposition microflora, nitrogen fixation microflora, a pH buffering agent, bacterial cellulose, a curing agent and a soil water retention agent. The nutritional soil for the vegetation cultivation of the karst landform, disclosed by the invention, has the advantages that good growth soil can be provided for the vegetation of the karst landform, the problem of stony desertification of the karst-landform regions can be effectively solved, and virtuous cycle for ecosystems of the karst-landform regions is promoted.

The invention discloses an underground water nitrogen-fixation remediation filtering material and a preparation method thereof, belonging to the field of underground water remediation. The filtering material is prepared from the following components in percentage by weight: 10-50% of plastic binding mineral, 1-10% of permeable substrate and 40-80% of inorganic cation exchange substrate or inorganic anion exchange substrate. The preparation method comprises the following steps: preparing raw materials according to the percentage by weight, and pulverizing the plastic binding mineral and the inorganic cation exchange substrate or inorganic anion exchange substrate to 200 meshes for later use; pulverizing the permeable substrate to 50 meshes for later use; thoroughly mixing the pulverized raw materials, granulating, and rolling to obtain balls; and calcining the obtained ball-shaped filtering material. The remediation filtering material can be combined with other biological curing materials, and has the technical advantages of low water resistance coefficient, almost no short stream or circle stream, and the like.

The invention relates to a liquid composite biological multi-control fertilizer. The invention is characterized in that the liquid composite biological multi-control fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of Streptomyces hygroscopicus solution, 1-3 parts of Streptomyces cinnamofuscus solution, 2-4 parts of Bacillus amyloliquefaciens solution, 2-4 parts of bacillus sphaericus solution, 7-15 parts of Bacillus mucilaginosus solution, 1-3 parts of Azotobacter chroococcum solution, 1-3 parts of Streptomyces aureochrogmoenes, 8-10 parts of Trichoderma viride fermentative degradation solution, 8-10 parts of matrix and 45-65 parts of sterilized water. The invention prepares a crop-absorbing carbohydrate and organic matter Trichoderma viride fermentative degradation solution, and provides high-efficiency beneficial microbial florae with phosphate solubilizing, potassium solubilizing, nitrogen fixation and disease resistance effects. The fertilizer can simultaneously perform the three functions of biological pesticide, biofertilizer and biological nutrients, and has the advantages of no toxicity or harm and no environment pollution. Compared with other fertilizers with the same nutrients, the crop yield is increased by 20%.

The invention discloses a low-cost boron-containing steel and a manufacturing method thereof. The steel comprises the following chemical components by weight: 0.05%-0.08% of C, 0.10-0.25% of Si, 1.60%-1.80% of Mn, 0.03%-0.045% of Als, 0.030%-0.045% of Ti, 0.003%-0.009% of B, less than or equal to 0.025% of P, less than or equal to 0.015% of S, less than or equal to 0.0015% of O, less than or equal to 0.0025% of N, and the balance Fe and unavoidable impurities. At the later stage of LF (ladle furnace) refining, by adding aluminum to perform deoxidation and adding ferrotitanium to undergo nitrogen fixation, the content of O and N in the steel can be controlled, and finally by adding ferroboron, the yield of boron and the distribution uniformity of boron in the steel can be guaranteed. Specifically, the heating temperature is at 1120-1180DEG C, and the heating time is 90-120min; the initial rolling temperature of hot rolling is 1090-1150DEG C, and the final rolling temperature is 790-850DEG C; the initial cooling temperature is 750-810DEG C, and the terminal cooling temperature ranges from (500-540)-30DEG C to (500-540)+30DEG C. In the invention, by control of boron in the steel to avoid combination with oxygen and nitrogen, the content of boron in solid solution in the steel can be ensured, and the reinforcement effect of boron can be improved. Through changing the adding amount of boron and adjusting the temperatures of heating, hot rolling and cooling, the low-cost boron-containing steel with strength levels of 460MPa, 500MPa and 550MPa can be obtained.

The invention discloses a solid composite biological multi-control water and moisture retention fertilizer and a preparing method and application thereof.The fertilizer is prepared from bentonite, matrix, streptomyces hygroscopicus liquid, bacillus megatherium liquid, bacillus amyloliquefaciens liquid, bacillus sphaericus liquid, bacillus mucilaginosus liquid, azotobacter chroococcum liquid, bacillus cereus liquid, bacillus subtilis liquid, golden streptomyces chromogenes liquid, streptomyces microflavus liquid and trichoderma viride liquid.The preparing method comprises the steps of 1, preparing bacterial liquid; 2, mixing and drying the different kinds of bacterial liquid and the matrix, and then mixing with bentonite for pelletizing.The fertilizer can achieve nitrogen fixation, dephosphorization and potassium release, absorb water to maintain soil moisture, promote soil organic matter decomposition, resist bacteria and insects, improve microbiota in soil and improve the structure of soil.

The invention discloses a photocatalyst. The photocatalyst is the indium oxide/indium sulfide heterojunction semiconductor material with a molecular formula of In2O3/In2S3 for solar nitrogen fixation.The preparation method of the catalyst comprises the steps of 1, using water or an organic solvent as a solvent; 2, dissolving an indium salt compound, a surfactant and a weakly basic substance in the solvent; 3, placing in a reaction kettle to perform a hydrothermal reaction; 4, cooling to the room temperature, centrifuging and alternately washing with water and ethanol solution, and drying to obtain indium hydroxide; 5, calcining the indium hydroxide in a muffle furnace to obtain indium oxide; 6, placing the indium oxide in the solvent, stirring and conducting ultrasonic treatment; 7, adding a sulfur source, and stirring; 8, placing into the reaction kettle for a secondary hydrothermal reaction; 9, cooling to the room temperature for centrifugation separation and alternately washing with the water and ethanol solution, and drying to obtain the indium oxide / indium sulfide heterojunction semiconductor material. The photocatalyst is economical, environmentally friendly and recyclable, can be mass-produced, and has solar nitrogen fixation performance.

The invention discloses a preparation method of bio-organic fertilizer. The preparation method can produce selenium-rich silicon-rich bio-organic fertilizer. The bio-organic fertilizer has high organic selenium and organic silicon contents. Through the bio-organic fertilizer, crops can absorb a large amount of selenium and silicon. A complex microbial agent has effects of dissolving phosphorus, fixing nitrogen and improving soil fertility and significantly improves content of a variety of trace minerals in fruits. The preparation method has simple processes, realizes a low preparation cost and is suitable for industrial production. The bio-organic fertilizer does not easily lose nutrients and has lasting fertilizer efficiency. Through raw material fermentation, the fermentation product is rich in selenium so that selenium content of the crop fruits is improved, the fruit economic value is improved and planting benefits are significantly improved. The bio-organic fertilizer can significantly improve the selenium content of agricultural products and significantly increase the percentage of organic selenium in total selenium in the agricultural products, is suitable for application of selenium deficient or low selenium regions in China and has the great significance to improve the selenium level. The complex microbial agent can play a role in phosphorus dissolution, nitrogen fixation and soil fertility increasing and can significantly improve the various trace mineral contents of fruits.