557 results about "Nitrogen fixation" patented technology

The invention relates to the technical field of agricultural biology, and discloses an auxiliary cultivation method for promoting sugarcane biological nitrogen fixation, which comprises the following steps: (1) breeding a sugarcane variety with high nitrogen-fixing bacteria affinity, and preparing an environmental stress resistant nitrogen-fixing bacteria agent; (2) carrying out acidification improvement and carbon source pre-burying on the planting soil; (3) inoculating the nitrogen-fixing bacterial agent to the sugarcane seed stem or rhizosphere area; (4) maintaining the metabolic activity of nitrogen-fixing bacteria by dynamically regulating and controlling rhizosphere carbon source supply and oxygen partial pressure; (5) adjusting a fungicide supplementary application strategy in real time based on the environmental data; and (6) solidifying the symbiotic character through intergenerational epigenetic treatment. Through host-microorganism coevolution and intelligent dynamic regulation and control, the nitrogen fixation efficiency and environmental adaptability of the sugarcane-nitrogen-fixing bacteria symbiotic system are remarkably improved; and epigenetic and flora iteration technologies are combined, so that stable transmission of cross-generation characters is realized, and a low-consumption and high-benefit ecological solution is provided for sustainable agriculture.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention belongs to the technical field of microorganisms, and provides a growth-promoting P.adami M6 and application thereof, the P.adami M6 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M20242835, the preservation date is December 18, 2024, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, and the preservation number is CCTCC No: M20242835. The strain is separated from rhizosphere soil of field planting foxtail millet in the Inner Mongolia red peak area, and is obtained through artificial separation and purification; the strain has the growth promoting characteristics of nitrogen fixation, phosphorus dissolution, IAA production and siderophore production; the M6 fungicide is used for inoculating millet, so that the growth of millet seedlings can be obviously promoted, and the plant height, the root length and the overground part and underground part biomass of the millet are obviously increased; m6 is used as a microbial agent to soak seeds during sowing and irrigate a bacterial liquid once 7 days after sowing, so that the growth effect of millet is best.

Burkholderia, and a composition, use and usage method thereof. Provided are the Burkholderia, and a composition comprising the strain and / or a metabolite, culture, fermentation broth and / or extract thereof. The Burkholderia or the composition thereof can generate a siderophore, induce plants or seeds thereof to generate systemic resistance to plant pathogens, and reduce the chemotaxis of plant pathogens to plants, so that prevention and treatment of plant diseases and insect pests are achieved. In addition, plant root system development can be promoted, a nitrogen fixation effect is provided, a phosphate solubilization effect is provided, indoleacetic acid is generated, and weeds are removed, thereby promoting plant growth.

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plants life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

The invention belongs to the technical field of rhizosphere microorganisms, and provides a strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens and application of the strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens in growth promoting and repairing of saline-alkali soil. The strain not only grows fast, but also has growth promoting functions such as nitrogen fixation, phosphorus solubilization and potassium solubilization. The strain is preserved in the China General Microbiological Culture Collection Center on March 11, 2025, called CGMCC for short, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the biological preservation number of the strain is CGMCC NO.33793. The SP-38 strain has multiple growth promoting functions, and through salt stress water culture tests and in-situ saline-alkali soil pot tests of corn, the strain has good effects in the aspects of improving the crop yield and repairing saline-alkali soil.

The invention relates to the technical field of microorganisms, in particular to application of bacillus glucans and a complex microbial inoculant thereof in preparation of bio-organic fertilizers, the strain Mh85 is separated from the root system of apple rootstock Malus hupehensis treated by a research group through trichoderma TB2, has good IAA production, nitrogen fixation, inorganic phosphorus decomposition and siderophore production capacities, and can be used for preparing bio-organic fertilizers. The strain Mh85 not only can directly promote plant growth, but also has a good growth-promoting effect on trichoderma TB2, and when the strain is compounded with the trichoderma TB2 to form a complex microbial inoculant, tomato seedling pot experiments are carried out. The complex microbial inoculant shows a synergistic effect, effectively overcomes the problems of unstable growth promoting effect, insufficient synergistic effect among strains and the like of an existing single microbial preparation, provides an efficient and stable technical scheme for improving plant growth, improving root development and increasing crop yield, and is a good raw material for preparing a bio-organic fertilizer.

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

The invention discloses a Brucella with antibacterial activity and application of the Brucella. The invention discloses Burkholderia sp.HNIV0701, and the preservation number of the Burkholderia sp.HNIV0701 is GDMCC No: 66007. The Burkholderia sp.HNIV0701 disclosed by the invention has a stable, efficient and broad-spectrum antibacterial property, has an inhibition effect on a plurality of plant pathogenic bacteria such as Botryosphaeria dothidea, Phytophthora nicotianae, Fusarium solani, Alternaria alternata, Thanatepora cucurbitae, Ralstonia solanacearum and the like, and also can be used for inhibiting Staphylococcus aureus and / or Listeria monocytogenes, so that the Burkholderia sp.HNIV0701 can be used for inhibiting a plurality of plant pathogenic bacteria such as the Botryosphaeria dothidea, the Phytophthora nicotianae, the Fusarium solanum, the Alternaria solanacearum and the like. The Brucella HNIV0701 can hydrolyze organic phosphorus and can be used for soil improvement. The Brucella HNIV0701 has nitrogen fixation capability and has the potential of promoting plant growth.

Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

The invention relates to the technical field of environmental microorganisms, in particular to arthrobacterium Arth.R8 and application thereof, and the preservation number of the arthrobacterium Arth.R8 is GDMCC No.63567. The arthrobacter Arth.R8 has the effects of fixing nitrogen, dissolving organic phosphorus, promoting growth and promoting nodulation. The arthrobacter Arth.R8 disclosed by the invention can be used for promoting absorption and utilization of nutrient substances such as nitrogen and phosphorus in a plant growth process, inhibiting growth of fusarium graminearum and promoting plant growth, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention discloses plant probiotics with water retention, growth promotion and disease resistance effects and application thereof. The invention provides a complex microbial inoculant, which is prepared from the following active ingredients: 1) bacillus tequilensis Bac365 (GDMCC No: 65241), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) Enterococcus querebrasis Ent274 (GDMCC No: 65242) or Pseudomonas plecoglossa Pse350 (GDMCC No: 65243), and the active ingredients of the complex microbial inoculant are as follows: 1) bacillus tequilensis Bac365 (GDMCC No: 65243), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) the active ingredients of the complex microbial inoculant. The strain provided by the invention has good nitrogen fixation, phosphorus dissolution, IAA production, HA production and disease resistance, can effectively increase the utilization efficiency of water in drought soil, significantly promote plant growth and increase crop yield, and has good application prospects in the aspects of water retention and sand stabilization, agricultural production and the like.

The invention relates to the technical field of environmental microorganisms, in particular to Chryseobacterium Chry.R1 and application of the Chryseobacterium Chry.R1, and the preservation number of the Chryseobacterium Chry.R1 is GDMCC (China General Microbiological Culture Collection Center) No.63568. The Chryseobacterium Chry.R1 has the effects of fixing nitrogen, inhibiting fusarium graminearum and promoting plant growth. The Chryseobacterium Chry.R1 disclosed by the invention can be used for promoting the absorption and utilization of nitrogen elements in a plant growth process, inhibiting the growth of fusarium graminearum and promoting the growth of plants, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention discloses bacillus for preventing and treating anthracnose of camellia oleifera and application of the bacillus. The preservation number of the bacillus CH-1-7 provided by the invention is GDMCC (Glucose Digital Microorganism Center) No. 66219; the bacillus amyloliquefaciens has the capability of generating living-promoting substances, can generate organic phosphorus and siderophores, has the nitrogen fixation capability, can inhibit the growth of various pathogenic bacteria, especially colletotrichum gloeosporioides, can be used for preventing and treating camellia oleifera anthracnose, and has important application value in the field of plant protection.

The invention belongs to the technical field of microorganisms, and relates to a strain of herbaspirillum as well as a fermentation method and application thereof. The invention provides a strain of brassica juncea, the brassica juncea is brassica juncea YLHb25.7, the brassica juncea is named as Herbaspiril huttiense, the brassica juncea is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35297. The invention also provides a preparation method of the brassica juncea. The herbispirillum sp. YLHb25.7 disclosed by the invention has the capabilities of producing ACC deaminase, producing IAA and fixing nitrogen, and has good drought resistance and saline-alkaline tolerance. The herbaspirillum sp. YLHb25.7 and the fermentation product thereof can promote the growth of wheat and corn, can be used as a microbial agent or bacterial fertilizer, and can be applied to the agricultural market.

The invention relates to an application of a nano silicon dioxide combined flora in relieving plant salt stress, which comprises the following steps: applying nano silicon dioxide to soil in which soybeans are to be planted, then planting the soybeans in the soil, and adding a simplified salt-tolerant growth-promoting synthetic flora microbial inoculum; wherein the synthetic flora is separated from saline-alkali soil, the growth promoting functions of nitrogen fixation, phosphorus dissolving, potassium dissolving and the like of the synthetic flora are measured, and the growth promoting effect of a single bacterium is verified through a pot experiment. A simplified flora SynC1 is constructed, and the relieving effect of combined application of the nano silicon dioxide and the flora on soybean salt stress is evaluated through a pot experiment. It is found that combined application of nano-silica and SynC1 can better promote root development, increase the proline content and promote soybean growth under salt stress, and has excellent application potential in agricultural soybean production.

The invention provides mulberry enterobacter, a microbial agent as well as a preparation method and application of the microbial agent. The bacterial strain XJ 10 is classified and named as Enterobacter mori, and is preserved in the Guangdong Microbial Culture Collection Center on February 20, 2025, the preservation number is GDMCC No.65928, and the preservation date is February 20, 2025. The strain XJ 10 can tolerate cadmium-lead combined pollution, has the capabilities of fixing nitrogen, dissolving phosphorus and dissolving potassium, can promote residual cadmium and lead in soil to be converted into effective cadmium and lead, can promote absorption of enrichment plants or hyperaccumulator to cadmium and lead, and can improve the physicochemical properties of the soil at the same time.

The invention relates to Hunan pseudomonas and application thereof. The pseudomonas hunanensis JP1-10 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the pseudomonas hunanensis JP1-10 is CGMCC (China General Microbiological Culture Collection Center) NO: 33318. The strain disclosed by the invention has relatively high salt resistance, has the functions of producing siderophores, IAA, nitrogen fixation and phosphate solubilization, can help plants relieve salt stress and promote growth, and also can antagonize pathogenic bacteria of fusarium oxysporum of cucumbers. Wide application prospects are realized.

The invention relates to the technical field of industrial microorganisms, and particularly discloses a P.megatherium HHY-1, a microbial agent thereof and application of the P.megatherium HHY-1. The preservation number of the P.megaterium HHY-1 is CGMCC (China General Microbiological Culture Collection Center) No.34470, and the preservation number of the P.megaterium HHY-1 is CGMCC No.34470. The P.megatherium provided by the invention has multiple functions of nitrogen fixation, phosphorus solubilization, siderophore production, IAA production capacity, antioxidant enzyme activity and the like, can play a comprehensive role from multiple dimensions of nutrition supply, growth regulation, stress resistance protection and the like, effectively assists good growth of the Abajie wheat 19 in a saline-alkali environment, and has a good application prospect. The method has great significance in improving the yield of the drought alkaline wheat in the saline-alkali soil and promoting agricultural sustainable development, and shows extremely high practical value in the aspect of growth promotion of the drought alkaline wheat Gie wheat 19.

The invention discloses a growth-promoting rhizobacteria, a growth-promoting rhizobacteria microbial inoculum and application thereof, and belongs to the technical field of microorganisms, the growth-promoting rhizobacteria is Pseudomonas mohnii, the strain number is YQ142, the classification name is Pseudomonas mohnii, and the growth-promoting rhizobacteria is preserved in China General Microbiological Culture Collection Center on April 7, 2025, and the preservation number is CGMCC No.34110. The invention further discloses a preparation method of the growth-promoting rhizobacteria. The growth-promoting rhizobacteria has strong nitrogen fixation and phosphate solubilizing capabilities, can promote the growth of ryegrass, oat and corn, and improves the saline-alkali stress resistance and drought stress resistance of ryegrass.

The invention belongs to the field of agricultural microorganisms, and particularly relates to coxakella cocori and application thereof. The coxakia cokei is preserved in the China General Microbiological Culture Collection Center, the classification name of the coxakia cokei is HHQGLZ30-1 (Kosakia cokei HHQGLZ30-1), the preservation date of the coxakia cokei is January 29, 2024, and the preservation number of the coxakia cokei is CGMCC No.29798. The coxakia cokei is named as the coxakia cokei HHQGLZ30-1. The coxakella cocori can secrete auxin, has nitrogen fixation capacity and siderophore production capacity, can convert iron elements which are difficult to absorb and utilize by plants in soil into siderophore chelated iron, and can promote plant growth and antagonize phytopathogens. By applying the growth-promoting and disease-preventing microbial agent prepared from the Corisakia cocori HHQGLZ30-1, the stress resistance of crops can be effectively improved, the growth of the crops is promoted, the crops are healthier, and the leaf surfaces are brighter and greener.

The invention discloses a compound microbial organic fertilizer and a preparation method thereof, and belongs to the technical field of compound microbial organic fertilizers. The compound microbial organic fertilizer is prepared from the following raw materials in parts by weight: 8-12 parts of a microbial agent, 30-35 parts of livestock and poultry manure, 20-25 parts of crop straws, 10-15 parts of humic acid, 10-12 parts of bagasse carbon, 3-5 parts of rumen bypass fat powder, 3-5 parts of molasses and 1-3 parts of chitosan oligosaccharide. According to the compound microbial organic fertilizer, the nitrogen-fixing pseudomonas, the streptomyces elongisporus and the paenibacillus polymyxa are selected to be compounded, and the three strains comprehensively promote root development, nutrient absorption and stress resistance of strawberry plants through the synergistic effect of nitrogen fixation, nutrient activation, hormone secretion and disease inhibition. The compound microbial organic fertilizer can effectively promote growth of strawberry plants, improve the quality of strawberries, increase the yield, reduce chemical fertilizer and pesticide residues, reduce the risk of soil pollution and meet the requirements of consumers for safe and organic strawberries.

The invention discloses a Klebsiella Michaeli MX-56 strain and application thereof, and belongs to the technical field of biological agriculture. The Klebsiella michelianensis MX-56 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 66361, the preservation time is May 19, 2025, and the preservation address is No. 100, Xianlie Middle Road, Guangzhou City. The invention also discloses an application of the Klebsiella michelianensis MX-56 in preparation of a divaricate saposhnikovia root growth promoting agent and a method for promoting growth of divaricate saposhnikovia root by using the Klebsiella michelianensis MX-56. A root irrigation method is adopted to irrigate MX-56 bacterial liquid. The divaricate saposhnikovia root endophytic bacterium Klebsiella michelianensis MX-56 disclosed by the invention has the growth promoting characteristics of fixing nitrogen, dissolving inorganic phosphorus, and secreting IAA (Indoleacetic Acid) and an iron-producing carrier; the strain can be stably colonized in the soil around the divaricate saposhnikovia roots; a good growth promoting effect is achieved on wind prevention.

The invention provides a method for repairing degraded moso bamboo forest soil. The method comprises the following steps: selecting a degraded moso bamboo forest; the moso bamboo forest is divided into a plurality of strip-shaped areas according to a high-density-low-density classification mode, and high-density areas and low-density areas which are distributed alternately are formed; the moso bamboo forest is subjected to strip-shaped thinning treatment, strip-shaped areas are divided according to a high-density-low-density classification mode, and moso bamboos of different growth ages are subjected to thinning according to a certain proportion; performing soil preparation treatment on the moso bamboo forest after thinning; planting nitrogen-fixing plants in the soil under the moso bamboo forest; and treating the soil under the moso bamboo forest by using a soil conditioner. According to the method for restoring the degraded moso bamboo forest soil, arbor layer-under-forest-soil integrated restoration is achieved, systematic improvement is conducted on multiple links of thinning, nitrogen fixation and fertilization, the content of N in the soil and the content of organic matter in the soil are increased, and the diameter at breast height of new bamboo is increased.

The invention discloses a preparation method and application of an iron-based metal organic framework material / carbon nitride nitrogen fixation photocatalyst in the field of photocatalytic materials, the iron-based metal organic framework material / carbon nitride nitrogen fixation photocatalyst is prepared by taking ferric chloride, terephthalic acid and graphite phase carbon nitride as raw materials through a hydrothermal reaction, a heterojunction is constructed through a solvothermal method, uniform loading of MIL-53 (Fe) on the surface of b-C3N4 is achieved, and the photocatalytic performance of the photocatalyst is improved. And the formed heterogeneous interface significantly improves the photon-generated carrier separation efficiency. The obtained MIL-53 (Fe) / b-C3N4 photocatalytic composite material has good visible light response characteristic and excellent photocatalytic nitrogen fixation performance, and is safe, non-toxic, green and environment-friendly. The produced ammonia water can be directly absorbed by wheat, so that the chlorophyll content of leaves is increased by 11-13%, the plant height is increased by 19-21%, and a novel photocatalysis scheme is provided for green agriculture.

The invention discloses an enterobacter strain and application thereof in improving stress resistance of plants. The invention relates to the field of biological agriculture, and provides Enterobacter sphaeroides YJD-AD2-2, and the enrollment number of the Enterobacter sphaeroides YJD-AD2-2 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.29524. The Enterobacter sphaeroides YJD-AD2-2 can be used for preparing the enterobacter sphaeroides YJD-AD2-2. The enterobacter sphaeroides YJD-AD2-2 provided by the invention can improve the resistance of arabidopsis thaliana, alfalfa, corn and rice to salt and alkali stress, specifically, the dry weight, fresh weight, plant length, leaf area and chlorophyll content are increased, the oxidation resistance is improved, wilt is reduced, and physiological and biochemical indexes are improved. The YJD-AD2-2 microbial inoculum improves the property and activity of saline alkali soil and improves the abundance and composition of microbial communities. Detection shows that the YJD-AD2-2 strain has the capabilities of secreting IAA (Indoleacetic Acid), ACC (Acetyl Cellulose) deaminase and siderophore, dissolving phosphorus and fixing nitrogen. The indexes show that the YJD-AD2-2 microbial inoculum has the potential of growth-promoting bacteria and has important application value in the field of agriculture.

The invention discloses a distributed plasma and electrocatalytic synthesis ammonia device which comprises a plasma nitrogen fixation unit, an electrocatalytic synthesis ammonia unit and a power supply module, and the plasma nitrogen fixation unit comprises an air pump, a plasma discharge reactor, a plasma power supply and an aeration head. The electro-catalytic ammonia synthesis unit comprises a cathode side liquid storage tank, a cathode side liquid pump, an ammonia production electrolytic bath, an anode side liquid pump, a direct-current power supply and an anode side liquid storage tank, and the power supply module is a clean energy module. The aeration head is immersed in the cathode side liquid storage tank, and electrolyte is stored in the cathode side liquid storage tank and the anode side liquid storage tank; the device takes air and water as raw materials and carries out a synthesis ammonia process at normal temperature and normal pressure. The method has the beneficial effects that the method adapts to clean energy, can realize on-site absorption and adapts to the volatility of the clean energy; raw materials are cheap and easy to obtain, hydrogen storage and transportation risks and extra cost pollution are avoided, and zero carbon is generated in the whole process; the device is small in scale and modularized, does not depend on large-scale facilities, and assists ammonia and chemical fertilizer production in regions deficient in resources.

The invention belongs to the technical field of agriculture and relates to microorganisms, in particular to a compound microbial agent and application thereof. The invention relates to a compound microbial agent, which is prepared from slow-growing rhizobium HS3, pseudomonas Y43, burkholderia Y30 and agrobacterium Y27. The compound microbial agent NBI prepared from efficient rhizobium and beneficial microbial strains adapting to local soil is preferably selected by combining soil types and microbial adaptability of different regions in Hainan province, and can be used for improving nodulation and nitrogen fixation capacity of fresh soybeans, promoting the yield of the soybeans, reducing application of chemical fertilizers and improving the per unit yield of the soybeans.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plant's life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.