274 results about "Growth promotion" patented technology

The invention discloses a potassium fulvate chelated macroelement water-soluble fertilizer with stress resistance and growth promotion functions, and relates to the technical field of water-soluble fertilizers, the potassium fulvate chelated macroelement water-soluble fertilizer comprises the following raw materials by weight: 167-210 parts of mineral source potassium fulvate, 450-460 parts of potassium nitrate, 300-310 parts of urea, 150-160 parts of potassium dihydrogen phosphate, 30-40 parts of potassium sulfate, 15-25 parts of EDTA-disodium, and 5-15 parts of citric acid. 0.5 to 1.5 parts of brassinolide and 4 to 6 parts of polyaspartic acid; according to the potassium fulvate chelated macroelement water-soluble fertilizer with the stress-resistant and growth-promoting functions, through chelation cooperation of potassium fulvate and nitrogen, phosphorus and potassium, the solubility of the chelated phosphorus element in water is increased, and the chelated phosphorus element can be more uniformly distributed in soil; by adopting an EDTA-disodium and citric acid composite chelating system, volatilization and loss of nitrogen elements are reduced, the problem of precipitation of calcium, magnesium and phosphorus is solved, and the soil conditioner is suitable for hard water areas and especially has outstanding performance in saline-alkali soil and arid areas.

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention discloses a preparation process of high-efficiency absorption fish protein peptide and application of the high-efficiency absorption fish protein peptide in fertilizer, and belongs to the technical field of marine organisms. The aquatic product protein is directly used as a fermentation raw material, and the content of amino acid in the fish protein biological peptide is remarkably increased by utilizing the synergistic fermentation effect of microorganisms and assisted by biological enzymolysis, so that the absorptivity of the fish protein peptide is increased; the prepared fish protein peptide is used in the fertilizer to prepare the fish protein peptide functional fertilizer, the nutritional value and the utilization rate of the fertilizer are remarkably improved, and the fish protein peptide functional fertilizer has the remarkable effects of growth promotion, disease resistance, stress resistance and the like. According to the method, a conventional fish protein peptide preparation method of firstly preparing fermentation enzyme liquid and then performing biological enzymolysis is abandoned, the efficient absorption fish protein peptide is prepared by adopting a process of directly fermenting aquatic protein, a fermentation process and an enzymolysis process are integrated, and compared with a conventional process, the method has the advantages of simple production process, low production cost and the like; the method has significant effects and meanings for promoting green preparation of the fish protein peptide and efficient application of the fish protein peptide in fertilizers.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention discloses a molybdenum oxide nanorod material, a preparation method of the molybdenum oxide nanorod material and application of the molybdenum oxide nanorod material to improvement of corn nutrient utilization, growth promotion and lodging resistance, and belongs to the technical field of corn nutrient utilization and agricultural nano material application. The morphology and dispersity of the nanorod are optimized by combining molecular composition characteristics of key enzymes (such as nitrate reductase and glutamine synthetase) in a corn nutrient utilization process, and finally the functional nanorod with targeting property and biocompatibility is obtained and can be widely applied to nutrient regulation and control scenes of corn water culture and field planting. After the material is applied, the activity of nitrate reductase and glutamine synthetase of a corn root system can be activated in a targeted manner, the nutrient utilization efficiency is accurately improved, and sufficient nutrition support is provided for plant growth. After the fertilizer is applied to plants, the root system is promoted to be developed, the stalks are thick and strong, the lodging resistance is remarkably enhanced, the protein content of the plants and grains can be increased, and the synergistic effects of efficient nutrient utilization, robust plant growth, lodging resistance and yield increase are achieved.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention discloses paenibacillus polymyxa FBN-001 and application thereof, the preservation number of the paenibacillus polymyxa FBN-001 is CCTCC NO: M 20251416, the paenibacillus polymyxa FBN-001 has the characteristics of multiple growth promoting functions and environmental adaptability, by means of the paenibacillus polymyxa FBN-001, crop growth can still be obviously promoted under the nitrogen-reducing fertilization condition, the nitrogen utilization efficiency is improved, and the application of the paenibacillus polymyxa FBN-001 is promoted. The soil micro-ecological environment is improved, so that stable yield and yield increase of crops are realized, chemical fertilizer dependence is reduced, and green agriculture and sustainable development are promoted.

The invention belongs to the technical field of agricultural fertilizers, and discloses a preparation process and application of a medical stone double-effect organic fertilizer with soil improving and growth promoting functions, the medical stone double-effect organic fertilizer comprises the following components by weight: 20-40 parts of activated medical stone powder with a particle size of less than or equal to 0.1 mm, and activated by a citric acid solution; the decomposed organic material is prepared by mixing livestock and poultry manure and crop straw according to the mass ratio of 2: 1-4: 1 and performing aerobic composting fermentation through a compound microbial agent, and the water content is smaller than or equal to 30%; the microbial agent contains phosphate solubilizing bacteria, nitrogen-fixing bacteria and rhizosphere growth-promoting bacteria, and the effective viable count is greater than or equal to 2 * 10 CFU / g; the auxiliary material comprises bentonite, a polyacrylamide water-retaining agent and a pH (Potential of Hydrogen) regulator, and the proportion of the bentonite is greater than or equal to 30 percent. The adsorption-desorption performance of the medical stone is enhanced by activating the medical stone through citric acid, and a synergistic system of water retention, fertilizer supply and growth promotion is formed by combining decomposed organic materials and functional microorganisms. According to the fertilizer, the stability of a soil aggregate structure can be remarkably improved (increased by 15-20%), the yield increasing rate of crops reaches 20-30%, and 30% of chemical fertilizer dosage is reduced. The method is suitable for acid soil remediation, continuous cropping obstacle treatment and economic crop planting.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention belongs to the technical field of microorganisms, and particularly relates to a pseudomonas TaRb6 capable of preventing diseases and promoting growth and application of the pseudomonas TaRb6. The TaRb6 strain provided by the invention belongs to a potential new species of pseudomonas, and the preservation number of the TaRb6 strain is CGMCC (China General Microbiological Culture Collection Center) No.36131. The strain TaRb6 and a fermentation inoculant prepared from the strain TaRb6 have good disease prevention and growth promotion functions on plants; the bacterial inhibition rate of sterile fermentation liquor of the strain TaRb6 on fusarium pseudograminearum reaches 46.54%-62.82%, and the bacterial colony diameter of the fusarium pseudograminearum can be reduced by 61.38% through metabolized volatile organic compounds of the strain TaRb6. After the TaRb6 bacterial suspension is used for seed soaking treatment of wheat, the incidence rate of wheat basal stem rot can be remarkably reduced, and the growth vigor of wheat can be promoted. In addition, the strain TaRb6 has broad-spectrum antibacterial ability, and the antibacterial rate of the strain TaRb6 on pathogenic bacteria of fusarium, botrytis cinerea, apical cyst and xanthomonas reaches 47.23%-67.02%.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

The invention belongs to the field of fungus secondary metabolites and application thereof, and particularly relates to trichoderma reesei HK1-12 as well as a secondary metabolite and application thereof, the collection unit is China General Microbiological Culture Collection Center (CGMCC), and the collection number is CGMCC No. 41951. The trichoderma HK1-12 has excellent characteristics in the aspects of growth promotion, plant protection and the like, and is expected to replace the existing similar products to be popularized and applied.

This invention discloses a composite microbial agent with both efficient ferulic acid degradation and growth-promoting functions, and its preparation method. The active component of this composite microbial agent contains *Pseudomonas soybeanii* with accession number CGMCC No. 36468. Pseudomonas soyae Strain A9, and *Pseudomonas aeruginosa* with accession number CGMCC No. 36469. Paraburkholderia oxyphila Strain A23. This invention, by screening and combining two functionally complementary strains, fundamentally solves the technical problems of existing single-strain ferulic acid degrading bacteria agents having limited function and unstable field application efficacy, achieving improved degradation efficiency and synergistic growth promotion effects.

The invention discloses a compound vitamin amino acid premix as well as a preparation method and application thereof, and belongs to the technical field of feed additives. An embedding system is formed by the chitosan, the polyethylene glycol and the glyceryl ricinoleate, and the tea tree oil and the perilla oil are compounded to serve as an antioxidant, so that the stability of vitamins can be remarkably improved. Besides, hydroxyapatite is added into the starch, modification treatment is performed through succinic anhydride and polyvinylpyrrolidone, the component compatibility is good, o-vanillin is introduced, the fragrance of o-vanillin has a food calling effect, the palatability is improved, the components of the functional additive are optimized, and the remarkable immune enhancement and growth promotion effects can be achieved; the cellular immunity and humoral immunity functions of the body are enhanced, and the granulating formability is effectively promoted by combining with the components of the carrier with good compatibility. On the whole, through interaction of the vitamin composition, the amino acid composition, the functional additive and the carrier, the immunity and growth performance of animals are jointly improved from multiple aspects.

The invention provides a strain of underground neosphingobacterium AR5-1 and application thereof in growth promotion and drought resistance, and the strain is named as Novosphingobacterium subraneum in taxonomy, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.35273. The invention also provides a method for preparing the underground neosphingobacterium AR5-1. The invention further provides an application of the underground neosphingobacterium AR5-1 in growth promotion and drought resistance. The underground neosphingosine bacterium is separated from tobacco rhizosphere drought stress soil, and through molecular identification (the similarity of 16S rRNA is 100%) and functional verification, the underground neosphingosine bacterium has the capacity of producing indoleacetic acid (the concentration of IAA is 0.142 + / -0.005 mg / mL) and fixing nitrogen. A bacterial suspension (OD600 = 0.3) prepared from the bacillus subtilis is applied to plant rhizosphere, so that the growth of crops such as tobacco and the like can be remarkably promoted (the biomass is increased by more than or equal to 20%), the drought resistance is enhanced (the drought rehydration recovery rate is increased by 35%), and the bacillus subtilis has the advantages of environmental friendliness and reduction of chemical fertilizer dependence, and is suitable for sustainable agricultural development.

The invention discloses a strain of bradyrhizobium guangzhouense APHBXY-23-1 with functions of efficiently promoting growth and strongly inhibiting aspergillus flavus and application of the strain of bradyrhizobium guangzhouense APHBXY-23-1. The preservation number of the strain of bradyrhizobium guangzhouense APHBXY-23-1 is CCTCC (China Center for Type Culture Collection) M 20253048. The invention not only retains the core functions of the strain as rhizobium for efficient symbiotic nitrogen fixation and crop growth promotion, but also reveals that the strain can significantly inhibit the growth of aspergillus flavus by releasing a volatile substance dimethylbutyric acid for the first time. The discovery breaks through the technical limitation that the traditional rhizobium only has a growth promoting function and lacks a biocontrol effect, the two agricultural microorganism core functions of nitrogen fixation and growth promotion and biocontrol bacteriostasis are endogenously integrated into the same microorganism carrier for the first time, and a novel rhizobium resource with double functions is successfully constructed; a new species of growth-promoting rhizobium with a biocontrol function is created, and the growth-promoting rhizobium has important innovative value in theory and application levels.

The application discloses Paracoccus communis C4-4 and C5-3, the preservation number of which is CGMCC NO:31971 and the classification name of which is Paracoccus communis, and the preservation number of which is CGMCC NO:31972 and the classification name of which is Paracoccus communis. Researches show that C4-4 and C5-3 have strong ecological adaptability and good growth promotion ability, and can improve the internal environment of soil in combination with organic materials. A synthetic microbial population constructed by Paracoccus communis comprises C4-4 and C5-3. Tests prove that the synthetic microbial population comprising C4-4 and C5-3 has the advantages of the synthetic microbial population, the microbial population has high alkali production and growth promotion ability, has good ecological adaptability, the pH adaptation range is 4-8, the manganese stress tolerance can reach 5000 μmol / L, can significantly improve the pH of a culture medium, and has obvious alkalization effect. The synthetic microbial population is used as a microbial agent and is compounded with organic materials to prepare a soil conditioner capable of improving acid soil and promoting plant growth. The conditioner is applied to acid and lean soil, can significantly improve the soil pH, improve the content of soil organic matter, nitrogen, phosphorus and potassium, and promote root growth.

The invention belongs to the technical field of pollution control, and relates to a soil pollution treatment method and system utilizing microbial remediation, and the method comprises the following steps: obtaining target remediation functional microbial agent genome and to-be-remedied site native microbiome metagenome data, and constructing a biological information basic data set; processing the data, and constructing an interaction network model containing microbial inoculum and native species nodes based on metabolic complementarity and ecological niche overlapping degree simulation calculation; analyzing the network topology structure to screen a key native co-generation node set, and generating a growth promotion demand map; determining a targeted metabolism regulation factor with targeted selectivity based on atlas reverse matching, and generating a targeted signal instruction; executing the instruction and putting a microbial agent, activating a synergistic node in situ and coupling with the microbial agent to construct a degradation function network; the method solves the problem that the colonization efficiency of the exogenous functional microbial inoculum is not high due to lack of accurate regulation and control on the native microbial community.

The application provides a jelly-like paenibacillus and application thereof, the jelly-like paenibacillus (Paenibacillus mucilaginosus) is preserved in China General Microbiological Culture Collection Center on February 8, 2025, and the preservation number is CGMCC No.33505.The jelly-like paenibacillus of the application has excellent phosphorus and potassium dissolving capacity, effectively promotes crop growth and improves crop yield.Meanwhile, the jelly-like paenibacillus can prevent and treat ginger wilt and root rot of ginger, inhibit the growth of pathogenic bacteria of ginger, prevent and treat diseases of ginger, realize "growth promotion-bacterium inhibition" dual, reduce the dependence of ginger on chemical pesticides, and increase the yield of ginger.

A use of an amino acid peptide solution produced by decomposition of fish scales using Bacillus velezensis for healthy and strong growth of Asteraceae seedlings and growth promotion of Asteraceae plants is provided. The Bacillus velezensis is deposited in Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH with an accession number of DSM 34894. The Bacillus velezensis is used to decompose fish scales and produce an amino acid peptide solution containing peptides and amino acids. The amino acid peptide solution can make the Asteraceae seedlings grow healthy and strong and promote growth of the Asteraceae plants effectively and specifically.

The application discloses a breeding method for improving the quality of Sebastes schlegelii fry, and comprises the following steps: collecting parent fish in a Sebastes schlegelii original seed field, artificially cultivating the parent fish in a net cage at sea, and performing nutrition rejuvenation on the parent fish; obtaining offspring fry by using an artificial fry squeezing method; controlling reasonable culture density according to different fry specifications; screening and using natural plant active ingredients and common micro-ecological preparations which are easy to absorb; avoiding disinfection and drug use; and controlling water flow rate in different growth stages. The method integrates nutrition rejuvenation, artificial fry production, pollution-free water quality regulation and fry behavior state regulation, solves problems such as poor parent germplasm, dead fry or insufficient activity of fry, slow growth and poor stress resistance in the process of fry breeding, has strong controllability in the operation period, has high survival rate of fry, has remarkable growth promotion and immunity effect, has good prevention and treatment effect on bacterial diseases, realizes pollution-free fry breeding, can reduce disease occurrence and mortality, reduces the feed conversion ratio, and effectively improves the economic benefit of breeding.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

The invention provides application of beta-1, 4-glucan type cellooligosaccharide in improvement of plant rhizosphere micro-ecology and enhancement of IAA signals, and belongs to the technical field of biological agriculture. The invention provides an application of beta-1, 4-glucan type cellooligosaccharide with the polymerization degree of DP2-DP5 in improvement of plant rhizosphere micro-ecology and enhancement of IAA (Indole Acetic Acid) signals. In the invention, beta-1, 4-glucan type cellooligosaccharide can induce plant immunity in a growth allowable PTI threshold range, so that a rhizosphere microflora structure is directionally remodeled, the relative abundance of beneficial microorganisms is improved, the potential pathogen load is reduced, rhizosphere IAA and related metabolites thereof can be increased, and'disease resistance without growth inhibition 'is realized. The invention further provides an application of the beta-1, 4-glucan type cellooligosaccharide combined with rhizosphere beneficial microorganisms in biological agriculture, and growth promotion and / or nodulation nitrogen fixation effects can be enhanced.

The application discloses a composite microbial inoculant prepared from a salt and alkali resistant strain Y3, wherein the composite microbial inoculant comprises bacteria and fungi; the bacteria are the salt and alkali resistant strain Y3 with a preservation number of CCTCC NO: M 20251595; and the fungi are a strain NJAU4742 with a preservation number of CGMCC NO.12166. The application also discloses application of the composite microbial inoculant in promoting growth of crops in a salt and alkali resistant manner. The salt and alkali resistant strain Y3 screened by the application has good salt and alkali resistance, IAA production and other functions; the Y3 single microbial inoculant prepared from the strain Y3 has better growth promotion capacity on crops under a salt and alkali condition; and the composite microbial inoculant prepared from the strain Y3 and the strain NJAU4742 has better growth promotion capacity on crops under a salt and alkali condition. The application provides a strain resource and technical support for promoting growth of crops in a salt and alkali area.

The invention discloses a strain of pseudomonas gestacei LAB-SA 50, which is classified and named as pseudomonas gestacei, the preservation date is September 18, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35979. The strain of pseudomonas gestacei LAB-SA 50 is named as pseudomonas gestacei LAB-SA 50. The invention also discloses a microbial inoculum prepared from the strain. The invention further discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis in growth promotion of saline-alkali soil crops, and the saline-alkali soil is soda type saline-alkali soil. The screened pseudomonas georgi LAB-SA 50 has relatively strong salt resistance and alkali resistance, and can colonize and stably exert functions in a soda type saline-alkali stress environment, so that the damage of soda type saline-alkali stress to crops (such as corn) is effectively relieved, and the growth of the crops (such as corn) is promoted.

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The invention discloses a stenotrophomonas Chinese angelica rhizosphere growth-promoting bacterium with bolting resistance, the bacterium is obtained by separating, purifying and culturing rhizosphere soil in which umbelliferae plant Chinese angelica grows, the bacterium is identified as stenotrophomonas lactiti SL1202409 through microorganism 16S rRNA (ribosomal ribonucleic acid) gene sequence sequencing, the bacterium is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20252606. Potting and field tests show that the separated Stenotrophomonas lactiti SL1202409 has a bolting resisting function and a Chinese angelica growth promoting function, Chinese angelica can be promoted to produce ferulic acid, the bolting rate of Chinese angelica can be remarkably reduced by applying the microbial agent in the field compared with negative control without applying the microbial agent, the biomass of Chinese angelica in different development stages is increased, and the yield of Chinese angelica is increased. The strain can be used as a special microbial fertilizer for angelica sinensis and has important application prospects.

The invention relates to the field of acid soil conditioners, in particular to a charcoal-based acid soil conditioner as well as a preparation method and application thereof. The biochar-based acid soil conditioner comprises a biochar carrier and a complex microbial inoculant loaded on the biochar carrier, and the complex microbial inoculant comprises 25-35 parts of nitrogen-fixing functional bacteria; 20 to 40 parts of phosphorus and potassium solubilizing bacteria; 15 to 25 parts of nitrogen activating bacteria; 15 to 25 parts of synergistic growth promoting bacteria; the nitrogen-fixing functional bacteria comprise Azospirillum brasilense and / or Klebsiella, the phosphate-solubilizing and potassium-solubilizing bacteria comprise bacillus megatherium and / or bacillus mucilaginosus, the nitrogen-activating bacteria comprise urease-producing bacillus and / or nitrospirillum, and the synergistic growth-promoting bacteria comprise arbuscular mycorrhizal fungi and / or pseudomonas. The complex microbial inoculant is constructed by screening four functional strains of nitrogen fixation, phosphorus and potassium solubilization, nitrogen activation and synergistic growth promotion, a complete functional closed loop of nitrogen fixation, phosphorus solubilization, nutrient activation and crop growth promotion is formed, and synergistic interaction is achieved through functional complementation of the strains.

The invention relates to a caffeine-resistant burkholderia paracasei and application of the caffeine-resistant burkholderia paracasei in tobacco growth promotion, the caffeine-resistant burkholderia paracasei is named as a YIM B11874 strain, the preservation number of the caffeine-resistant burkholderia paracasei is CGMCC No.36352, and the preservation date is October 27, 2025. The bacterial strain has good nitrogen fixation and phosphorus solubilization capabilities, and pot experiments show that the bacterial strain has a good growth promotion effect on crops represented by tobacco, and has a positive construction effect on sustainable development of agriculture and environmental protection.

The invention provides bacillus altitudinis JZBQ5 and application of the bacillus altitudinis JZBQ5 in plant disease prevention and growth promotion. The preservation number of the bacillus altitudinis JZBQ5 is CGMCC (China General Microbiological Culture Collection Center) The bacillus altitudinis JZBQ5 can improve the disease resistance of strawberries, and has a good control effect on powdery mildew and anthracnose of strawberries. Meanwhile, growth of cucumbers and wheat is promoted, and plant diseases such as cucumber root rot and wheat stem rot are effectively prevented and controlled. The strain provides a new strain resource for biological control of crop diseases such as strawberry powdery mildew, and a theoretical basis is provided for a safe and efficient green biocontrol preparation.