72 results about "Burkholderia" patented technology

The invention discloses an application of burkholderia in root system microorganisms in regulation and control of glycosylation of ginsenoside. The invention provides application of burkholderia or a composition containing the burkholderia in any one of the following applications: 1) regulating and controlling proportions of different glycosylated saponins in ginseng; 2) increasing the proportion of monoglycosylated saponin and / or the proportion of disglycosylated saponin in ginseng; 3) preparing ginseng with a high monoglycosylated saponin ratio and / or a high disglycosylated saponin ratio; and 4) improving ginseng cultivation. In a greenhouse environment, the roots of ginseng seedlings are inoculated with burkholderia, metabolome data shows that the burkholderia regulates glycosylation formation of ginsenoside, and the proportion of monoglycosylated saponin and / or the proportion of disglycosylated saponin can be increased. Further, transcriptome sequencing data analysis shows that the burkholderia regulates and controls the expression of different glycosyl transferases, so that the diversity of glycosylation of ginsenoside is influenced. The burkholderia provides a new thought for refined improvement of a ginseng cultivation technology.

The invention discloses radix notoginseng stem and leaf rare ginsenoside as well as a preparation method and application thereof, and belongs to the technical field of ginsenoside. The radix notoginseng stem and leaf rare ginsenoside comprises Rk3, Rh4, S-Rg3, R-Rg3, Rk1 and Rg5. The radix notoginseng stem and leaf rare ginsenoside can be used for preparing radix notoginseng stem and leaf rare ginsenoside. The preparation method comprises the following steps: mixing the pseudo-ginseng stem and leaf extract with an acetic acid aqueous solution, reacting at 105-120 DEG C for 10-40 minutes, and drying; in the preparation process, macroporous resin purification is not carried out. According to the scheme, part of chemical substances in the panax notoginseng stem and leaf extract can be rapidly converted at a low temperature, new active substances Rh4, S-Rg3, R-Rg3, Rk1 and Rg5 with high purity are obtained at the same time, and the obtained panax notoginseng stem and leaf rare ginsenoside can effectively inhibit propionibacterium acnes, burkholderia cepacia and malassezia furfur.

The invention relates to the technical field of bioengineering, and discloses Burkholderia lipase with T251S mutation and a recombinant expression vector and application thereof, threonine of the 251 amino acid sequence of LipA of wild Burkholderia lipase is mutated into serine. The enzyme activity of the mutant enzyme is remarkably improved by 23%, meanwhile, the hydrolysis capacity on ethyl ester type fish oil is remarkably improved, the selectivity on EPA is improved, and excellent EPA purification and enrichment effects are achieved. According to the mutant, the preparation cost is reduced, the hydrolysis capability and selectivity are improved, and the mutant has great popularization and application values.

The invention relates to the technical field of microorganisms, in particular to burkholderia metallica BW17 with weight-losing, disease-resisting and growth-promoting functions as well as a fungicide and application thereof, and the strain has good capabilities of producing IAA, fixing nitrogen, dissolving phosphorus and broad-spectrum antagonizing pathogenic bacteria. Pot experiments prove that the strain can significantly promote growth of pyrus betulaefolia and tomato seedlings, improve root morphology, increase biomass, and significantly improve appearance and internal quality of tomato fruits. In addition, the BW17 has an obvious inhibition effect on various phytopathogens. The strain can be widely applied to planting of crops such as fruit trees and vegetables as a bio-organic fertilizer or a microbial agent, partially replaces chemical fertilizers, reduces environmental pollution, improves the quality and yield of agricultural products, and has good popularization and application values.

The invention provides bacillus paralicheniformis BC20 from deep sea and application of the bacillus paralicheniformis BC20 in cosmetics. The bacillus paralicheniformis BC20 which is derived from deep sea and has broad-spectrum antibacterial activity is obtained through research, and the bacillus paralicheniformis BC20 has the characteristics of salt resistance, heat resistance and the like. A fermentation product and antibacterial peptide of the bacillus paralicheniformis BC20 have broad-spectrum antibacterial activity, and have a good inhibition effect on various bacteria and fungi. Particularly, the bacillus paralicheniformis BC20 disclosed by the invention shows a very good inhibition effect on polluted burkholderia for the first time. The bacillus paralicheniformis BC20, the fermentation product thereof and the antibacterial peptide can be used as bacteriostatic agents in the fields of cosmetics and the like.

The invention discloses an application of burkholderia polluting LZ816 in the aspect of preventing and treating fusarium and soil-borne diseases. The contaminated Burkholderia sp. LZ816 has relatively strong inhibitory activity on Fusarium sp. Such as Fusarium pseudograminearum, Fusarium oxysporum cucumber specialization, Fusarium laminatum, Fusarium oxysporum banana specialization, Fusarium solani, Fusarium oxysporum tomato specialization, Fusarium oxysporum white gourd specialization and the like; the bacterial strain can be used for well preventing and controlling wheat basal stem rot, cucumber fusarium wilt, soybean fusarium wilt, banana fusarium wilt, strawberry root rot, tomato fusarium wilt and white gourd fusarium wilt, and has important significance and application value for agricultural production.

The invention provides a method and a kit for detecting blood flow infection pathogens and application of the method and the kit. Specifically, the invention provides a primer probe combination for detecting bloodstream infection pathogens, and the primer probe combination comprises: (a) upstream and downstream primers and probes for detecting serratia marcescens as shown in SEQ ID NO: 7-9; (b) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 10-12 and are used for detecting burkholderia cepacia; (c) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 1-3 and are used for detecting stenotrophomonas maltophilia; and (d) upstream and downstream primers and a probe which are shown as SEQ ID NO: 4-6 and are used for detecting enterobacter cloacae. The primer probe combination provided by the invention can be used for realizing rapid, high-sensitivity and high-specificity detection of blood flow infection pathogens.

The invention relates to the technical field of microbial identification, in particular to a molecular marker for identifying burkholderia cepacia as well as a primer, a kit and application of the molecular marker. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO. 1. According to the detection primer, the kit and the application provided by the invention, the defect that the homology analysis resolution of the 16S rDNA sequence is insufficient in the existing method is overcome, the specific conserved gene sequence of the burkholderia cepacia is fully utilized, and a simple, rapid and economic identification method is established; the method can be used for detecting and identifying burkholderia cepacia pollutants in safety quality control of medicines, cosmetics and foods.

A process for producing polyhydroxyalkanoates, comprising the steps of: fermenting production medium comprising pongamia oil using bacteria from the Cupriavidus, Burkholderia, or Alcaligenes genera capable of producing polyhydroxyalkanoates; wherein the pongamia oil is extracted from the seeds of Pongamia species and clarified by filtering before the crude, unprocessed pongamia oil is added to the production medium; and extracting the polyhydroxyalkanoates comprising a homopolymer comprising a 3-hydroxybutyrate (3HB) unit amongst other sole PHB polymers from within the bacteria.

The present invention relates to a method for treating Gram-negative bacteria that are resistant to colistin (MIC > 50 mg / mL), an antibacterial compound, excluding those of the genera Burkholderia, Proteus, and Serratia, comprising contacting the bacteria with an aqueous pharmaceutical composition containing a rose bengal (RB) compound of formula I, dissolved or dispersed at a concentration of about 0.01 to about 15 mg / mL, and irradiating the contacted bacteria with light of a wavelength of about 500 nm to about 600 nm for a period of about 1 to about 10 minutes, at a concentration of about 16 to about 160 J / cm². 2 The aim is to create a method that involves obtaining a certain amount of light to treat and kill irradiated bacteria. [Formula 1] JPEG2026511692000028.jpg5864 (where X, R 1 , R 2 (and M+ are defined herein)

This invention discloses a strain of *Burkholderia lagoon* and its application in antagonizing *Aspergillus flavus*, belonging to the field of microbial technology. The *Burkholderia lagoon* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38387. This strain can both inhibit the growth of *Aspergillus flavus* and efficiently degrade aflatoxin B1, thus showing good application prospects and value in the field of food production safety technology.

In the present invention, three novel ethylenediamine-N, N '-disuccinic acid (EDS) synthases are provided, which are derived from the group consisting of Cholatococcus asaccharoporus, Sargassum pedis, and Burkholderia sarisoli, and, in the present invention, there are provided three novel ethylenediamine-N, N'-disuccinic acid (EDDS) synthases, which are derived from the group consisting of Cholatococcus asaccharoporus, Sargassum pedis, and Burkholderia sarisoli, and, in the present invention, there are provided three novel ethylenediamine-N, N '-disuccinic acid (EDDS) synthases, which are derived from the group consisting of Cholatococcus asaccharoporus. More particularly, provided are a novel polypeptide having EDDS synthase activity, a method for producing EDDS using an EDDS synthase, a method for producing a composition comprising EDDS, and a method for preparing an EDDS synthase.

The invention relates to the field of vegetable planting, in particular to a method for applying phosphate solubilizing bacteria extracted from tea garden soil to leaf lettuce growth promotion. The invention relates to a method for applying phosphate solubilizing bacteria extracted from tea garden soil to growth promotion of leaf lettuce, which comprises the following steps: selecting an activated strain, placing the strain in a triangular flask filled with an LB liquid culture medium, culturing on a reciprocating shaking table until bacterial liquid is slightly turbid, measuring the absorbance value of bacterial suspension at the wavelength of 600nm by using a spectrophotometer, measuring the OD600 value of the bacterial suspension to 0.7-0.9, and calculating the content of the phosphate solubilizing bacteria. A pipette is used for sucking bacterial liquid, 0.8% normal saline is added for constant volume, and then lettuce root irrigation treatment is carried out; the bacterial strain is one of bacillus velezensis, bacillus thuringensis, Burkholderia, Burkholderia, and Paenibacillus lentigera, and the bacterial strain is one of bacillus velezensis, bacillus thuringensis, Burkholderia, Burkholderia, and Paenibacillus lentigera.

The present invention relates to a compounds according to general formula (I), which acts as an inverse agonist of PqsR (the currently only known receptor for the Pseudomonas Quinolone Signal (PQS), sometimes also referred to as MvfR); to a pharmaceutical composition containing one or more of the compound(s) of the invention; to a combination preparation containing at least one compound of the invention and at least one further active pharmaceutical ingredient; and to uses of said compound(s), including the use as a medicament, e.g. the use in the treatment or prophylaxis of a bacterial infection, especially a Pseudomonas aeruginosa or Burkholderia infection.

The invention relates to the technical field of bioengineering, and discloses Burkholderia lipase with T132A mutation and a recombinant expression vector and application thereof, threonine of the 132 amino acid of wild Burkholderia lipase LipA is mutated into alanine. The expression quantity of the mutant enzyme is increased by 46%, meanwhile, the hydrolysis capacity on ethyl ester type fish oil is improved, the selectivity on EPA is improved, and excellent EPA purification and enrichment effects are achieved. According to the mutant, the preparation cost is reduced, the hydrolysis capacity and selectivity are improved, and the mutant has wide application and popularization value.

The application discloses a broad-spectrum growth promoting bacterial agent combination for oil camellia and oil tea, a biological preparation and application thereof, and relates to the field of microbial technology.The endophytic bacteria are separated from healthy oil camellia plants, the abilities of the strains in dissolving phosphorus, fixing nitrogen, producing IAA, producing iron carrier and antagonizing the pathogenic bacteria of oil tea anthracnose are determined, the onion Burkholderia and Aspergillus niger with excellent comprehensive growth promoting functions are screened to prepare a combined bacterial agent, and the combined bacterial agent is verified to have a significant growth promoting effect on the oil camellia and the oil tea through a potting experiment.The combined bacterial agent promotes the growth, nutrient absorption and root development of the oil tea, significantly improves the enzyme activities related to the carbon, phosphorus and nitrogen cycles of the soil, and realizes the overall optimization of the "plant-soil" system.The application finds a broad-spectrum mixed bacterial agent suitable for the artificial planting of the oil camellia and the oil tea in a rocky desertification area, effectively improves the adaptability of the plants in the rocky desertification habitat, and provides a new microbial technical approach for the collaborative repair of multiple tree species in the area.

The invention relates to the technical field of bioengineering, and discloses Burkholderia lipase with four mutations of P113A, Q88S, T132G and L287S as well as a recombinant expression vector and application of the Burkholderia lipase. Proline of the 113th amino acid sequence of the LipA of the wild burkholderia lipase mutates into alanine, glutamine of the 88th amino acid sequence of the LipA of the wild burkholderia lipase mutates into serine, threonine of the 132th amino acid sequence of the LipA of the wild burkholderia lipase mutates into glycine, and leucine of the 287th amino acid sequence of the LipA of the wild burkholderia lipase mutates into serine. The yield of the mutant enzyme is improved by 63%, the enzyme activity is improved by 34%, the hydrolysis capacity on ethyl ester type fish oil is improved by 38%, the selectivity on EPA is obviously enhanced, and the EPA purification and enrichment effect is excellent. According to the mutant, the preparation cost is reduced, the hydrolysis capability and selectivity are improved, and the mutant has great popularization and application values.

Provided in the present invention are a mutant of Burkholderia cepacia lipase, and a recombinant expression vector thereof and the use thereof. The mutant comprises P131S, T132A, T251A, T251S, P131A, P131Q, T132G, Q88S or P113A single mutation, Q88S and P113A double mutation, and combinations of multi-site mutations including combination 1 of Q88S, P113A, T132G and L287S, combination 2 of Q88S, P113A, P131S and L287S, and combination 3 of Q88S, P113A, P131S and T251A.

The invention relates to the technical field of bioengineering, and discloses Burkholderia lipase with P131Q mutation and a recombinant expression vector and application thereof, and proline of the 131 amino acid sequence of LipA of wild Burkholderia lipase is mutated into glutamine. The yield of the mutant enzyme is increased, the enzyme activity is improved, meanwhile, the hydrolysis capacity on ethyl ester type fish oil is remarkably improved by 44%, the selectivity on EPA is improved, and excellent EPA purification and enrichment effects are achieved. According to the mutant, the preparation cost is reduced, the hydrolysis capability and selectivity are improved, and the mutant has great popularization and application values.

The invention discloses a mixed microbial agent and application thereof in codonopsis pilosula planting. The composite flora is composed of phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria and phosphate solubilizing bacteria, potassium bacteria: pseudomonas aeruginosa and bacillus circulans; and nitrogen-fixing bacteria: autotrophic flavobacterium, clostridium pasteurianum and bradyrhizobium. Wherein the phosphate-solubilizing bacteria are wrapped by adopting a benzene diboronic acid-sodium alginate pH response material, the potassium-solubilizing bacteria are wrapped by adopting selenized sodium alginate-Ca < 2 + > salt ion response gel, and attapulgite is used as a carrier to form the slow-release bacterial agent. Phosphorus-solubilizing bacteria and potassium-solubilizing bacteria have a synergistic effect to maintain the optimal growth environment of codonopsis pilosula, trichoderma harzianum colonization is promoted through bacillus circulans exopolysaccharide to construct a synergistic network, and the acting effect of the fungicide is improved by combining dual-environment response release and boron-selenium element synergy. During application, the microbial inoculum and rotten stems and leaves of codonopsis pilosula are fermented to prepare a bacterial fertilizer for basal application, and then topdressing and flushing application are performed, so that the yield and polysaccharide content of codonopsis pilosula are remarkably increased.

The invention discloses a recombinant escherichia coli strain with high mutation frequency and a construction method and application thereof, and the construction method comprises the following steps: connecting a double-chain cytosine deaminase coding gene DddA from burkholderia to a plasmid pACYC Duet-1 through a seamless cloning technology to obtain a recombinant plasmid pACYC-tDddA-1416; the method comprises the following steps: on the basis of pACYC-tDddA-1416, introducing S1416P mutation by virtue of a cyclization PCR (Polymerase Chain Reaction) technology, so as to obtain a recombinant plasmid pACYC-tDddA-S1416P with high mutation frequency; the method comprises the following steps: carrying out chemical transfer to obtain escherichia coli BL21-pACYC-tDddA-S1416P; the method comprises the following steps: on the basis of pACYC-tDddA-1416, introducing S1416P mutation by virtue of a cyclization PCR (Polymerase Chain Reaction) technology, so as to obtain a recombinant plasmid pACYC-tDddA-S1416L with high mutation frequency; and the escherichia coli BL21-tDddA S1416L is obtained through chemical transfection. The recombinant escherichia coli strain with high mutation frequency provided by the invention can be used for adaptive evolution, and the method is simple and convenient.

The invention belongs to the technical field of microbial bioconversion, and particularly relates to application of Burkholderia sp. WJ-02 in ginsenoside conversion and a conversion method, and the Burkholderia sp. WJ-02 can convert ginsenoside Rb3 into ginsenoside Rd and rare ginsenoside F2. The Burkholderia sp. WJ-02 is separated from rhizosphere soil of American ginseng, the Burkholderia sp. WJ-02 can efficiently and selectively convert main ginsenoside Rb3 into ginsenoside Rd and rare ginsenoside F2 by taking American ginseng stem and leaf saponin as a substrate, and conversion target products are concentrated, so that subsequent purification is facilitated.

The invention relates to the technical field of bioengineering, and discloses Burkholderia lipase with Q88S mutation and a recombinant expression vector and application thereof, glutamine of the 88th amino acid sequence of LipA of wild Burkholderia lipase is mutated into serine. And the yield of the mutant enzyme is increased by 46%, the hydrolysis capability on ethyl ester type fish oil is improved, the selectivity on EPA is improved, and excellent EPA purification and enrichment effects are achieved. According to the mutant, the preparation cost is reduced, the hydrolysis capability and selectivity are improved, and the mutant has great popularization and application values.

The present application relates to the field of genetic engineering and enzyme engineering, in particular to a lipase mutant capable of esterifying short-chain fatty acids in aqueous phase, a construction method and application thereof. Based on a Burkholderia-derived lipase, two single-point mutants H24T and A191M and a double-point combination mutant H24T-A191M are screened. The mutant can catalyze the esterification reaction of isoamyl alcohol and acetic acid under high water activity conditions, and the esterification product is less affected by hydrolysis. The mutant obtained by the present application is more suitable for industrial production than the natural lipase, and can be particularly used in the field of removing fusel oil and catalyzing aging of liquor, and has good application prospect and industrial value.

The invention relates to the technical field of medicinal chemistry, in particular to a preparation method of Corey lactone diol. The invention provides a preparation method of Corey lactone diol. The preparation method comprises the following steps: S1, carrying out enzymatic reaction on a compound as shown in a formula II under the action of lipase to obtain a compound as shown in a formula III; s2, hydrolyzing the compound shown in the formula III to obtain a compound shown in a formula I; the lipase is selected from at least one of Candida antarctica lipase, Pseudomonas fluorescens lipase, Burkholderia lipase, Rhizopus mirabilis lipase and porcine pancreatic lipase. The method has the advantages of mild and simple reaction conditions, cheap and easily available raw materials, few reaction byproducts, high chiral optical purity of the product, short overall reaction steps and suitability for industrial production, simplifies the subsequent preparation process of the prostaglandin drugs, and reduces the production cost.

The invention provides a composition of multiple PCR primers and probes for detecting microorganisms, a kit and a method thereof. The composition comprises 10 pairs of primers and 10 corresponding probes for staphylococcus aureus, salmonella, pseudomonas aeruginosa, clostridium tetani, citrobacter freundii, stenotrophomonas maltophilia, burkholderia cepacia, klebsiella pneumoniae, candida albicans and escherichia coli, the 10 pairs of primers are as shown in SEQ ID No.1-20, and the 10 probes are as shown in SEQ ID No.21-30. The invention further discloses a kit for detecting the staphylococcus aureus, the salmonella, the pseudomonas aeruginosa, the clostridium tetani, the citrobacter freundii, the stenotrophomonas maltophilia, the burkholderia cepacia, the klebsiella pneumoniae, the candida albicans and the escherichia coli. The composition of the primer and the probe is used for detecting microorganisms through multiple PCR, operation is easy and convenient, and the detection accuracy rate is high.

The invention provides a lung cancer noninvasive screening system and method based on expiration microbiomics, and belongs to the technical field of biomedical detection.The method comprises the steps that a sample is obtained through an expiration collector, a bacteria abundance matrix is generated through a UMI-5R-16S sequencing technology, and the low-biomass expiration microorganism detection rate is remarkably increased; the method comprises the following steps of: constructing three types of hierarchical random forest models: M1 model: healthy human vs. Early lung cancer (I-II stage), and outputting early risk probability based on markers such as Ochrobactrum and the like; m2 model: healthy human vs. Middle and advanced stage lung cancer (III-IV stage), and advanced stage risk probability is output based on Burkholderia and other markers; m3 model: healthy human vs. Full lung cancer (including morning and evening), outputting a comprehensive risk score based on Dietzia and other markers; and M3, primarily screening positive samples, inputting the positive samples into M1 and M2, and performing staging evaluation, wherein a higher risk probability is taken as a criterion during conflict. According to the invention, the total lung cancer primary screening sensitivity is improved, and the early detection rate is increased; the microorganism detection rate of an expiration sample is high; and the single detection cost is lower.

The present invention relates to the field of bioengineering technology. The present invention discloses mutants of Burkholderia lipase, their recombinant expression vectors and applications. The mutants include single mutations of P131S, T132A, T251A, T251S, P131A, P131Q, T132G, Q88S, P113A; double mutations of Q88S and P113A; and multi-site mutation combinations: Combination 1: Q88S, P113A, T132G, L287S; Combination 2: Q88S, P113A, P131S, L287S; Combination 3: Q88S, P113A, P131S, T251A. Based on experimental findings, each mutant can not only increase the protein yield, but also improve the enzyme activity, with the highest increase of 57%. The EPA enrichment experiment found that the mutant can increase the EPA content by up to 33% at most, and has excellent EPA purification and enrichment effects.

The invention relates to the technical field of bioengineering, and discloses Burkholderia lipase with P113A mutation and a recombinant expression vector and application thereof, and proline of the 113th amino acid sequence of LipA of wild Burkholderia lipase is mutated into alanine. And the yield of the mutant enzyme is increased by 30%, the hydrolysis capability on ethyl ester type fish oil is improved, the selectivity on EPA is improved, and excellent EPA purification and enrichment effects are achieved. According to the mutant, the preparation cost is reduced, the hydrolysis capability and selectivity are improved, and the mutant has great popularization and application values.

The invention discloses an application of Burkholderia stabilizing bacteria in preparation of nano-selenium. According to the invention, the red nano-selenium is prepared by stabilizing a Burkholderia BX1 strain and carrying out biosynthesis. The invention discloses that the BX1 strain has selenite reducing capacity and can tolerate selenite with high concentration; the strain can efficiently convert sodium selenite to generate nano-selenium, the conversion rate is high, the conversion rate is high, 85.5% of sodium selenite can be converted within 2 hours, and the synthesized nano-selenium particles are uniform. And the content of the nano-selenium obtained by conversion in a liquid culture medium containing 5 mM of sodium selenite is 351.22 mg / kg. Meanwhile, the biosynthesized nano-selenium has a very good control effect on tobacco diseases, and the control effect on tobacco sclerotium rolfsii reaches 76.47%. The preparation method of biosynthetic nano-selenium provided by the invention is simple and convenient, high in efficiency, low in cost, high in product purity and environment-friendly, and provides more microbial strain resources for biosynthetic nano-selenium.