8 results about "Burkholderia" patented technology

A process for producing polyhydroxyalkanoates, comprising the steps of: fermenting production medium comprising pongamia oil using bacteria from the Cupriavidus, Burkholderia, or Alcaligenes genera capable of producing polyhydroxyalkanoates; wherein the pongamia oil is extracted from the seeds of Pongamia species and clarified by filtering before the crude, unprocessed pongamia oil is added to the production medium; and extracting the polyhydroxyalkanoates comprising a homopolymer comprising a 3-hydroxybutyrate (3HB) unit amongst other sole PHB polymers from within the bacteria.

This invention discloses a strain of *Burkholderia lagoon* and its application in antagonizing *Aspergillus flavus*, belonging to the field of microbial technology. The *Burkholderia lagoon* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38387. This strain can both inhibit the growth of *Aspergillus flavus* and efficiently degrade aflatoxin B1, thus showing good application prospects and value in the field of food production safety technology.

The application discloses a broad-spectrum growth promoting bacterial agent combination for oil camellia and oil tea, a biological preparation and application thereof, and relates to the field of microbial technology.The endophytic bacteria are separated from healthy oil camellia plants, the abilities of the strains in dissolving phosphorus, fixing nitrogen, producing IAA, producing iron carrier and antagonizing the pathogenic bacteria of oil tea anthracnose are determined, the onion Burkholderia and Aspergillus niger with excellent comprehensive growth promoting functions are screened to prepare a combined bacterial agent, and the combined bacterial agent is verified to have a significant growth promoting effect on the oil camellia and the oil tea through a potting experiment.The combined bacterial agent promotes the growth, nutrient absorption and root development of the oil tea, significantly improves the enzyme activities related to the carbon, phosphorus and nitrogen cycles of the soil, and realizes the overall optimization of the "plant-soil" system.The application finds a broad-spectrum mixed bacterial agent suitable for the artificial planting of the oil camellia and the oil tea in a rocky desertification area, effectively improves the adaptability of the plants in the rocky desertification habitat, and provides a new microbial technical approach for the collaborative repair of multiple tree species in the area.

This invention belongs to the fields of plant genetic engineering and agricultural biotechnology, and discloses the application of the OsCCR21, OsPAL05, OsPAL1, OsPAL07, and 4CL1 genes in regulating the rice phyllosphere microbiome and resistance. The nucleotide sequence of the OsCCR21 gene is shown in SEQ ID NO:1, the OsPAL05 gene in SEQ ID NO:2, the OsPAL1 gene in SEQ ID NO:3, the OsPAL07 gene in SEQ ID NO:4, and the 4CL1 gene in SEQ ID NO:5. This invention reveals that the OsCCR21, OsPAL07, OsPAL05, OsPAL1, and 4CL1 genes positively regulate the abundance of specific beneficial microbial orders (Pseudomonas or Burkholderia) in the rice phyllosphere, thereby enhancing the resistance of rice to different stresses such as sheath blight, brown planthopper, and rice blast.

This invention discloses a method for producing ascorbic acid tetraisopalmitate catalyzed by a combination of lipases, belonging to the field of bioenzyme catalysis. The method includes: reacting a combination of lipases containing seven specific sources with the substrate vitamin C and isopalmitic acid in a mixture of hydrophobic organic solvent and water to generate ascorbic acid tetraisopalmitate. The lipase combination is derived from Tocopheryl sulfide, human, Arabidopsis thaliana, yeast, Staphylococcus, Burkholderia, and soybean. This invention obtains a specific lipase combination that synergistically catalyzes the esterification of four hydroxyl sites on the vitamin C molecule by constructing a lipase library and using a single elimination method. Under optimized reaction conditions, the conversion rate reaches 91.8% in a 5L scale-up reaction. This method has mild reaction conditions, simple steps, high yield, and is environmentally friendly, making it suitable for industrial production.

The application discloses a kind of phenylboronic acid and dopamine grafted chitosan-manganese-based injectable hydrogel immunoadjuvant and its preparation method and application.The hydrogel takes chitosan as base material, constructs modified polymer by chemical grafting dopamine and phenylboronic acid;Manganese ion is loaded to form phenylboronic acid and dopamine grafted chitosan-manganese-based injectable hydrogel immunoadjuvant.The hydrogel is fixed antigen by physical adsorption, has the functions of targeted delivery, immune activation and controllable release, significantly enhances humoral immunity and cellular immunity.The hydrogel has the characteristics of low viscosity, supports simple injection, biodegradable and no local inflammatory reaction.Experiments show that the titer of the adjuvant is equivalent to that of Freund's adjuvant after immunizing mice, successfully used for preparing monoclonal antibody of gladiolus burkholderia, and constructs immune test strip, and the sensitivity reaches 5×10 5 CFU / mL.The application solves the problems of high toxicity, local damage and high cost of traditional adjuvant, and is suitable for vaccine development and immunodiagnosis.

This invention relates to the field of molecular diagnostics, specifically disclosing a composition for detecting Burkholderia melioides and its application. The composition includes PRA primers and a PRA probe. The PRA primers are used to amplify the T6SS1 effector protein gene of Burkholderia melioides. The PRA primers include a forward primer and a reverse primer. The forward primer is shown in SEQ ID No: 1; the reverse primer is shown in SEQ ID No: 2. The PRA probe is designed according to the amplification region of the PRA primers. The sequence size of the PRA probe is 46bp-54bp, its 5' end is labeled with FAM, its 3' end is modified with C3-Spacer, and THF is applied 34bp away from the 5' end in the sequence of the PRA probe. This composition provides a rapid detection method for Burkholderia melioides using the composition of this invention, which significantly improves sensitivity compared to PCR.

This invention provides a Burkholderia spp. bacterium, a synthetic bacterial community, and their application for the control of white rot disease in Coptis chinensis, belonging to the field of microbial technology. The Burkholderia spp. bacterium HJ23 has the preservation number CCTCC NO: M 20253076. This invention provides a synthetic bacterial community including Burkholderia spp. bacterium HJ23, Pseudomonas fluorescens, and Arthrobacter spp. This synthetic bacterial community can more effectively inhibit the growth of the pathogen causing white rot disease, controlling white rot in Coptis chinensis and peanuts. This synthetic bacterial community provides a novel and highly effective antagonistic strain resource for the green biological control of this soil-borne disease, and can effectively replace or reduce the use of chemical pesticides, reducing the risk of pesticide residues. This aligns with the development needs of green cultivation of Chinese medicinal herbs, and has significant practical value for ensuring the quality of Coptis chinensis, improving the economic benefits of Coptis chinensis cultivation, and promoting the sustainable development of the Coptis chinensis cultivation industry.