17 results about "Burkholderia" patented technology

The invention provides bacillus paralicheniformis BC20 from deep sea and application of the bacillus paralicheniformis BC20 in cosmetics. The bacillus paralicheniformis BC20 which is derived from deep sea and has broad-spectrum antibacterial activity is obtained through research, and the bacillus paralicheniformis BC20 has the characteristics of salt resistance, heat resistance and the like. A fermentation product and antibacterial peptide of the bacillus paralicheniformis BC20 have broad-spectrum antibacterial activity, and have a good inhibition effect on various bacteria and fungi. Particularly, the bacillus paralicheniformis BC20 disclosed by the invention shows a very good inhibition effect on polluted burkholderia for the first time. The bacillus paralicheniformis BC20, the fermentation product thereof and the antibacterial peptide can be used as bacteriostatic agents in the fields of cosmetics and the like.

A process for producing polyhydroxyalkanoates, comprising the steps of: fermenting production medium comprising pongamia oil using bacteria from the Cupriavidus, Burkholderia, or Alcaligenes genera capable of producing polyhydroxyalkanoates; wherein the pongamia oil is extracted from the seeds of Pongamia species and clarified by filtering before the crude, unprocessed pongamia oil is added to the production medium; and extracting the polyhydroxyalkanoates comprising a homopolymer comprising a 3-hydroxybutyrate (3HB) unit amongst other sole PHB polymers from within the bacteria.

The present invention relates to a method for treating Gram-negative bacteria that are resistant to colistin (MIC > 50 mg / mL), an antibacterial compound, excluding those of the genera Burkholderia, Proteus, and Serratia, comprising contacting the bacteria with an aqueous pharmaceutical composition containing a rose bengal (RB) compound of formula I, dissolved or dispersed at a concentration of about 0.01 to about 15 mg / mL, and irradiating the contacted bacteria with light of a wavelength of about 500 nm to about 600 nm for a period of about 1 to about 10 minutes, at a concentration of about 16 to about 160 J / cm². 2 The aim is to create a method that involves obtaining a certain amount of light to treat and kill irradiated bacteria. [Formula 1] JPEG2026511692000028.jpg5864 (where X, R 1 , R 2 (and M+ are defined herein)

This invention discloses a strain of *Burkholderia lagoon* and its application in antagonizing *Aspergillus flavus*, belonging to the field of microbial technology. The *Burkholderia lagoon* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38387. This strain can both inhibit the growth of *Aspergillus flavus* and efficiently degrade aflatoxin B1, thus showing good application prospects and value in the field of food production safety technology.

The application discloses a broad-spectrum growth promoting bacterial agent combination for oil camellia and oil tea, a biological preparation and application thereof, and relates to the field of microbial technology.The endophytic bacteria are separated from healthy oil camellia plants, the abilities of the strains in dissolving phosphorus, fixing nitrogen, producing IAA, producing iron carrier and antagonizing the pathogenic bacteria of oil tea anthracnose are determined, the onion Burkholderia and Aspergillus niger with excellent comprehensive growth promoting functions are screened to prepare a combined bacterial agent, and the combined bacterial agent is verified to have a significant growth promoting effect on the oil camellia and the oil tea through a potting experiment.The combined bacterial agent promotes the growth, nutrient absorption and root development of the oil tea, significantly improves the enzyme activities related to the carbon, phosphorus and nitrogen cycles of the soil, and realizes the overall optimization of the "plant-soil" system.The application finds a broad-spectrum mixed bacterial agent suitable for the artificial planting of the oil camellia and the oil tea in a rocky desertification area, effectively improves the adaptability of the plants in the rocky desertification habitat, and provides a new microbial technical approach for the collaborative repair of multiple tree species in the area.

The invention discloses a mixed bacterial agent ZL3 + 6 with effects of promoting growth of haloxylon ammodendron and regulating rhizosphere microflora and application of the mixed bacterial agent ZL3 + 6, and belongs to the technical field of microorganisms. According to the bacillus orientalis ZL3 and the bacillus cereus ZL6 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 and the ZL6 are mixed and applied to a haloxylon ammodendron field, the contents of hydrolytic nitrogen, available phosphorus and rapidly available potassium in soil and the activity of sucrase are remarkably improved and are respectively improved by 49.27%, 80.48%, 12.65% and 64.40%. The plant height is increased by 51.75%, and the root length is increased by 262.18%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, the ZL3 + 6 inoculant is inoculated to promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

This invention belongs to the fields of plant genetic engineering and agricultural biotechnology, and discloses the application of the OsCCR21, OsPAL05, OsPAL1, OsPAL07, and 4CL1 genes in regulating the rice phyllosphere microbiome and resistance. The nucleotide sequence of the OsCCR21 gene is shown in SEQ ID NO:1, the OsPAL05 gene in SEQ ID NO:2, the OsPAL1 gene in SEQ ID NO:3, the OsPAL07 gene in SEQ ID NO:4, and the 4CL1 gene in SEQ ID NO:5. This invention reveals that the OsCCR21, OsPAL07, OsPAL05, OsPAL1, and 4CL1 genes positively regulate the abundance of specific beneficial microbial orders (Pseudomonas or Burkholderia) in the rice phyllosphere, thereby enhancing the resistance of rice to different stresses such as sheath blight, brown planthopper, and rice blast.

The invention relates to the technical field of agricultural microorganisms, and discloses a plant rhizosphere bacillus for antagonizing plant pathogen pectobacterium carotovorum and application, the plant rhizosphere bacillus is bacillus velezensis Mp4-Ha31 and is preserved in the China Center for Type Culture Collection on September 30, 2025, and the preservation number is CCTCC NO: M 20252184. The invention further discloses a preparation method of the plant rhizosphere bacillus for antagonizing the plant pathogen pectobacterium carotovorum and application of the plant rhizosphere bacillus for antagonizing the plant pathogen pectobacterium carotovorum. The plant bacillus velezensis Mp4-Ha31 provided by the invention has the advantages that the antagonism capability on the bacterial plant pathogen pectobacterium carotovorum is relatively strong, the biological pesticide is developed by taking the bacillus velezensis Mp4-Ha31 as an object, and the potential application prospect is realized.

This invention belongs to the field of biomedical and analytical technology and provides an early screening model for esophageal cancer based on oral microorganisms and a method for constructing the same. [Solution] The early screening model uses the oral microbiome, including Prevotella, Granulicatella, Rothia, Prevotellamassilia, Blautia, Abiotrophia, Peptostreptococcus, Actinomyces, Burkholderia, Akkermansia, Fudania, Kineothrix, Bacillus, Duncanella, and Eisenbergiella, as markers. By identifying non-invasive biomarkers, asymptomatic esophageal cancer patients can be detected early.

The invention belongs to the technical field of microbiology and plant growth-promoting bacteria. More specifically, the invention relates to two efficient phosphorus-solubilizing burkholderia strains and application thereof. Two efficient phosphorus-solubilizing burkholderia strains, namely burkholderia metallica N19 and burkholderia cepacia N31, are separated from the citrus reticulata rhizosphere soil, and both the burkholderia metallica N19 and the burkholderia cepacia N31 can efficiently dissolve main insoluble aluminum phosphorus (Al-P) and calcium phosphorus (Ca-P) in the acid soil, so that the content of available phosphorus in the soil is remarkably increased. Pot experiments show that the N19 and N31 strains are compounded to irrigate roots, so that root development and overground part growth of Citrus chachiensis can be remarkably promoted, the phosphorus concentration of old leaves, new leaves and root systems is remarkably improved, and it is proved that the N19 and N31 strains enhance phosphorus absorption and transport capacity. The strain provided by the invention can adapt to a typical acidic, high-aluminum and organic-matter-rich rhizosphere micro-ecological environment, ensures long-acting expression of phosphate solubilizing and growth promoting functions under a complex soil background, and has a wide industrialization prospect and application value.

The application belongs to the technical field of microbial bioconversion, and particularly relates to application of Burkholderia WJ-02 in conversion of ginsenoside and a conversion method, wherein the Burkholderia WJ-02 can convert ginsenoside Rb3 into ginsenoside Rd and rare ginsenoside F2. The Burkholderia WJ-02 is obtained by being separated from rhizosphere soil of American ginseng, the strain can efficiently and selectively convert ginsenoside Rb3 into ginsenoside Rd and rare ginsenoside F2 with American ginseng stem and leaf saponin as a substrate, and the conversion target product is concentrated, so that subsequent purification is facilitated.

The invention relates to the technical field of agricultural microorganisms, and discloses a Burkholderia metablica BI1 strain and application thereof, the Burkholderia metablica BI1 strain is classified and named as Burkholderia metablica BI1, and the Burkholderia metablica BI1 strain is preserved in the China Center for Type Culture Collection on March 27, 2025 with the preservation number of CCTCC No: M2025612. The strain has broad-spectrum antagonistic activity on various pathogenic bacteria causing ginger soil-borne diseases, the bacteriostasis rate reaches 50%-85%, and insoluble phosphorus and potassium minerals can be dissolved. The obtained microbial preparation can be prepared into a liquid or solid form, the viable count is high, and the storage stability is good. Field tests prove that the disease occurrence rate of the ginger can be remarkably reduced, plant growth is promoted, and the yield is increased by 20-25%. The invention provides an efficient biological control and biological fertilizer integrated solution for green and sustainable cultivation of ginger and other crops.

This invention discloses a method for producing ascorbic acid tetraisopalmitate catalyzed by a combination of lipases, belonging to the field of bioenzyme catalysis. The method includes: reacting a combination of lipases containing seven specific sources with the substrate vitamin C and isopalmitic acid in a mixture of hydrophobic organic solvent and water to generate ascorbic acid tetraisopalmitate. The lipase combination is derived from Tocopheryl sulfide, human, Arabidopsis thaliana, yeast, Staphylococcus, Burkholderia, and soybean. This invention obtains a specific lipase combination that synergistically catalyzes the esterification of four hydroxyl sites on the vitamin C molecule by constructing a lipase library and using a single elimination method. Under optimized reaction conditions, the conversion rate reaches 91.8% in a 5L scale-up reaction. This method has mild reaction conditions, simple steps, high yield, and is environmentally friendly, making it suitable for industrial production.

The application discloses a kind of phenylboronic acid and dopamine grafted chitosan-manganese-based injectable hydrogel immunoadjuvant and its preparation method and application.The hydrogel takes chitosan as base material, constructs modified polymer by chemical grafting dopamine and phenylboronic acid;Manganese ion is loaded to form phenylboronic acid and dopamine grafted chitosan-manganese-based injectable hydrogel immunoadjuvant.The hydrogel is fixed antigen by physical adsorption, has the functions of targeted delivery, immune activation and controllable release, significantly enhances humoral immunity and cellular immunity.The hydrogel has the characteristics of low viscosity, supports simple injection, biodegradable and no local inflammatory reaction.Experiments show that the titer of the adjuvant is equivalent to that of Freund's adjuvant after immunizing mice, successfully used for preparing monoclonal antibody of gladiolus burkholderia, and constructs immune test strip, and the sensitivity reaches 5×10 5 CFU / mL.The application solves the problems of high toxicity, local damage and high cost of traditional adjuvant, and is suitable for vaccine development and immunodiagnosis.

This invention relates to the field of molecular diagnostics, specifically disclosing a composition for detecting Burkholderia melioides and its application. The composition includes PRA primers and a PRA probe. The PRA primers are used to amplify the T6SS1 effector protein gene of Burkholderia melioides. The PRA primers include a forward primer and a reverse primer. The forward primer is shown in SEQ ID No: 1; the reverse primer is shown in SEQ ID No: 2. The PRA probe is designed according to the amplification region of the PRA primers. The sequence size of the PRA probe is 46bp-54bp, its 5' end is labeled with FAM, its 3' end is modified with C3-Spacer, and THF is applied 34bp away from the 5' end in the sequence of the PRA probe. This composition provides a rapid detection method for Burkholderia melioides using the composition of this invention, which significantly improves sensitivity compared to PCR.

The invention belongs to the technical field of biological medicine and analysis, and provides an esophageal cancer early screening model based on oral microorganisms combined with oral health related behavior variables and a construction method thereof. The model is the following oral cavity microbial flora and oral cavity health related behavior variables: Prevotella, Granulicalala, Rothia, Prevotella, Blauria, Abiotrophia, Peptostreptococcus, Actinomyces, Burkholderia, Akkermansia, Fudania, Kineothrix, Bacillus, Duncaniella, Eisenbergiella, and a tooth brushing frequency, and the oral cavity health related behavior variables are selected from the group consisting of the following oral cavity microbial flora and oral cavity health related behavior variables. By identifying non-invasive biomarkers in combination with oral health related behavior variables, esophageal cancer patients without symptoms in the early stage can be found.

This invention provides a Burkholderia spp. bacterium, a synthetic bacterial community, and their application for the control of white rot disease in Coptis chinensis, belonging to the field of microbial technology. The Burkholderia spp. bacterium HJ23 has the preservation number CCTCC NO: M 20253076. This invention provides a synthetic bacterial community including Burkholderia spp. bacterium HJ23, Pseudomonas fluorescens, and Arthrobacter spp. This synthetic bacterial community can more effectively inhibit the growth of the pathogen causing white rot disease, controlling white rot in Coptis chinensis and peanuts. This synthetic bacterial community provides a novel and highly effective antagonistic strain resource for the green biological control of this soil-borne disease, and can effectively replace or reduce the use of chemical pesticides, reducing the risk of pesticide residues. This aligns with the development needs of green cultivation of Chinese medicinal herbs, and has significant practical value for ensuring the quality of Coptis chinensis, improving the economic benefits of Coptis chinensis cultivation, and promoting the sustainable development of the Coptis chinensis cultivation industry.