151 results about "Burkholderia" patented technology

The present invention relates to therapeutic nanoemulsion compositions and to methods of utilizing the same. In particular, nanoemulsion compositions are described herein that find use in the treatment and/or prevention of infection (e.g., respiratory infection (e.g., associated with cystic fibrosis)), in burn wound management, and in immunogenic compositions (e.g., comprising a Burkholderia antigen) that generate an effective immune response (e.g., against a bacterial species of the genus Burkholderia) in a subject administered the immunogenic composition. Compositions and methods of the present invention find use in, among other things, clinical (e.g. therapeutic and preventative medicine), industrial, and research applications.

Provided herein are systems for treating a subject with a pulmonary infection, for example, a nontuberculous mycobacterial pulmonary infection, a Burkholderia pulmonary infection, a pulmonary infection associated with bronchiectasis, or a Pseudomonas pulmonary infection. The system includes a pharmaceutical formulation comprising a liposomal aminoglycoside dispersion, and the lipid component of the liposomes consist essentially of electrically neutral lipids. The system also includes a nebulizer which generates an aerosol of the pharmaceutical formulation at a rate greater than about 0.53 gram per minute. The aerosol is delivered to the subject via inhalation for the treatment of the pulmonary infection.

It is intended to provide a novel NAD⁺-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD⁺; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The present invention is directed to a composition for decomposing a majority of food waste into water and carbon dioxide comprising an effective combination of at least two species of microorganisms chosen from bacillus, lactobacillus, burkholderia, yeast fungus, eumycetes or any combinations thereof. A preferred embodiment comprises a combination of four different species of microbes having DNA sequences that correspond to SEQ ID Nos. 5-8 and which were deposited with the Korean Collection for Type Cultures (KCTC) on Mar. 8, 2007, and designated KCTC11085BP; KCTC11086BP; KCTC11087BP; KCTC11088BP, respectively. Also, presented by this invention is a device and methods for decomposing a majority of food waste into water and carbon dioxide using the microbial compositions presented herein.

The invention discloses burkholderia multivorans WS FJ9 which is separated from the soil at the root of the pine and can dissolve the slightly-soluble inorganic phosphorus and organic phosphorus efficiently and the application of the burkholderia multivorans. The WS FJ9 burkholderia multivorans is collected in the CCTCC (China Center for Type Culture Collection) on December 1, 2011 and has a collection number of No. M2011435. The burkholderia multivorans WS FJ9 has strong tricalcium phosphate, aluminum phosphate and lecithin dissolving effect under the condition of submerged culture, is very different from the contrast, has strong sphaeropsissapinea inhibiting activity and can be prepared into a microbial inoculum inoculated to the wetland pine to promote the growth of the pine. The invention provided an excellent strain resource for the development of the biological fertilizer for the pine.

The invention relates to the field of microorganisms and discloses a microbial strain capable of remedying heavy metal-contaminated soil and application of the microbial strain. The preservation number of the microbial strain is CGMCC No.10843. A strain of burkholderia cepacia is screened from the soil contaminated by heavy metal cadmium and can purify the exchangeable cadmium in the soil and significantly reduce the content of the exchangeable cadmium. The microbial strain is extremely-strong in tolerance to the cadmium and small in soil disturbance, has a stronger biological remediation function, meanwhile can improve the activity of soil dehydrogenase and can also be applied to the remediation of the heavy metal-contaminated soil and the improvement of the activity of the soil dehydrogenase as well as the preparation of relevant products.

The invention discloses burkholderia for preventing and controlling rice sheath blight disease, which was identified and named burkholderia R456 and collected in China Center for Type Culture Collection (CCTCC) on 20th August, 2010, the CCTCC is located in Wuhan University, Luojia Mountain, Wuhan and the collection number is CCTCC No.M2010207. The burkholderia R456 in the invention has a relatively strong suppression effect on the growth of rhizoctonia solani under in-vitro, greenhouse and field conditions and has good prevention and control effect on rice sheath blight disease. Compared with onion burkholderia, the burkholderia R456 is not harmful to the plants and is very safe to be used as a biocontrol strain for preventing and controlling rice sheath blight disease, and thus, it is proved that the rhizosphere bacteria can be used as the biocontro strain for preventing and controlling rice sheath blight disease.

Provided are methods for identification of plant glutamine synthetase inhibitors as well as the plant synthetase inhibitors identified. Further provided are methods of modulating glutamine synthetase activity in a plant, particularly weeds by applying to the plant or substrate for cultivating the plant a microorganism (e.g. Burkholderia strain) in an amount effective to modulate glutamine synthetase activity.

The invention relates to a preparation method of a novel biological soil remediation agent. The method comprises the following steps: (1) compounding and mixing four bacteria, including anaerobic spore-bearing bacilli TIBCC1.002, micrococcus luteus TCCC23001, pseudomonas fluorescens AS1.867, Burkholderia cepacia NKCCMRNK.T2-2 according to the weight part ratio of (3-6) to (2-5) to (1-4) to (1-3) to form a compound bacterium solution, adding the compound bacterium solution according to the weight ratio of 1%-5% into active sludge, and biologically enhancing the active sludge; (2) filtering biogas slurry by a hollow fiber membrane with the pore diameter of 0.1-0.2 micron so as to obtain waste biogas slurry residues; (3) mixing the biologically enhanced active sludge with the waste biogas slurry residues according to the weight part ratio of (3-9) to (1-5), and stirring uniformly to form the novel biological soil remediation agent. The invention provides another approach for the recycling of the residual sludge and biogas slurry, so that not only can the polluted soil be remediated effectively, but also the load to the environment pollution by the residual sludge and the waste biogas slurry residues can be relieved to a certain extent; the preparation method has relatively high practical application value.

The invention discloses a whole-course biological control method of tobacco diseases and insect pests. The method comprises the following steps: 1) in the seedling stage: using predatory mites for controlling thrips and tomato spotted wilf viruses; using harmonia axyridis and aphidius gifuensis for controlling myzus persicae and the thrips; using penicillium to inactivate mycelia, and 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling tobacco mosaic diseases; 2) in the field period: using the predatory mites and harmonia axyridis for controlling the thrips, the tomato spotted wilf viruses and the myzus persicae; using sex attractants for controlling prodenia litura adults and helicoverpa assulta adults; using Cahtheconidea furcellata for controlling prodenia litura larvae; using 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling the tobacco mosaic diseases; using Holder Burkholderia, and Pythium oligandrum for controlling tobacco black shank, black root rot, root knot nematode diseases and the like. The whole-course biological control method of the tobacco diseases and insect pests disclosed by the invention thoroughly eliminates pollution of chemical pesticides on the environment and tobacco pesticide residues and effectively controls the diseases and insect pests continuously and effectively, and the loss of tobacco quality for the diseases and insect pests is controlled within 5%.

The invention belongs to the field of metallurgical technology, and relates to a decarbonizing and desulphurizing bacterium agent for a difficultly-selected gold ore. The bacterium agent is formed by mixing alicyclobacillus, acidithiobacillus ferrooxidans, pseudomonas aeruginosa, bacillus subtilis, bacillus megatherium and burkholderia bacteria. The invention further discloses the method for treating the difficultly-selected gold ore by utilizing the decarbonizing and desulphurizing bacterium agent. The bacterium agent provided by the invention can be used for treating the high-carbon high-sulfur gold ore containing the sulfur content above 15% and the carbon content above 8%, and greatly lowering the quantity of sodium cyanide required for extracting the gold in a cyaniding manner. As a result, the industrial cost is lowered.

Polynucleotides encoding the polypeptides involved in biosynthesis of FK 228 and those involved in synthesis of a novel FK228 analog, thailandepsin are disclosed herein. Also provided are methods of making FK228, thailandepsin and analogs of these molecules and methods of using these FK228 analogs. Chromobacterium and Burkholderia gene inactivation mutants are provided. Methods of forming a disulfide bond in a chemical are also disclosed.

The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.

The invention belongs to the technical field of metallurgy and relates to a refractory gold ore desulfurizing and dearsenifying method. A preparation used by the refractory gold ore desulfurizing and dearsenifying method is prepared from 30% of burkholderia, 20% of acidithiobacillus ferrooxidans, 20% of sulfolobus solfataricus, 20% of bacillus subtilis and 10% of pseudomonas aeruginosa. The microbial preparation used by the refractory gold ore desulfurizing and dearsenifying method is reasonable in compatibility and synergistic, so that a relatively good desulfurizing and dearsenifying effect can be achieved; the gold leaching rate is also greatly improved; and the microbial preparation can be recycled and is environment-friendly and pollution-free.

The present invention discloses a group of homologous recombinases capable of efficiently mediating the in vivo recombination of Burkholderia, wherein the homologous recombinase comprises a protein Red[alpha]7029 having the function of exonuclease, and a protein Red[beta]7029 having the function of single-stranded annealed protein. The invention further discloses an expression vector pBBR1-Rha-BA7029-Km containing the homologous recombinase, applications of the combination of the homologous recombinase and an E. Coli-derived double-stranded DNA exonuclease inhibitory protein Red[gamma] in improvement of the in vivo recombination efficiency of Burkholderia, and applications of the homologous recombinase in the obtaining of a compound glidopeptin or rhizopeptin. According to the present invention, the multiple genes can be iteratively deleted so as to simplify the genome of Burkholderia, or the functional promoter is inserted to activate the unknown organism in an in-situ manner to synthesize the gene cluster, and the like, such that the important significance is provided for the bio-exploration and the functional genomics study of Burkholderia.

The invention relates to a preparation method for burkholderia cepacia, and an application in high-performance phosphate-dissolving microbial fertilizer by using the same. The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. The phosphate-dissolving microbial fertilizer provided by the present invention has advantages of low cost, good effect, disease resistance of plants enhancing, no pollution on environment and fully utilization of potential phosphorus resource.

The invention relates to the technical field of Burkholderia, in particular to a Burkholderia (Burkholderia), which is deposited in a Chinese typical culture collection center, and the collection number is CCTCC NO: M 2018703. It has bacteriostatic effect on sheath blight, rice blast and bakanae.

A novel process for the remediation of nitrogenous energetic materials such as 1,3,5,-trinitro-1,3,5-triazine (RDX) which can be used in situ on contaminated media is provided. The process comprises the bioremediation by one or more miccroorganisms capable of metabolizing the energetic materials. Examples of such microorganisms include Rhizobium rhizogenes, Burkholderia sp., and Cladosporium cladosporioides (ATCC 66669). Strains of these microorganisms have been deposited. The strain designated A1 has been deposited as Rhizobium rhizogenes BL (ATCC PTA-4110) and the strain designated C8 has been deposited as Burkholderia sp. (ATCC PTA-4111) Additionally, with the addition of a carbon source, such as a sugar, the process can totally degrade the energetic materials in two to three days.

The invention discloses a preparation method of L-menthol. The preparation method comprises the following steps: 1) adding 3-methyl-6-isopropyl-phenol and a composite noble metal catalyst to a high-pressure kettle, wherein the composite noble metal catalyst is formed by the combination of a Raney nickel catalyst RTH211, palladium carbon, cyclodextrin and Cu/SiO2; closing the high-pressure kettle; filling hydrogen to replace the air in the kettle for two to three times; finally filling hydrogen to enable pressure in the kettle to be 5.0 to 8.0MPa; controlling the temperature to be 100 to 150 DEG C for 4 to 6 hours, thus obtaining a mixture of menthol; rectifying to obtain D,L-menthol; 2) performing esterification reaction on D,L-menthol and an acylation reagent under the effect of alkali so as to obtain D,L-menthol ester; 3) performing hydrolysis reaction on D,L-menthol ester under the effect of hydrolytic enzyme, thus obtaining L-menthol, wherein the hydrolytic enzyme is cellularized burkholderia cepacia (B.cepacia ATCC25416). The preparation method has the advantages that the production raw materials are simple and easy to obtain; the prepared L-menthol is high in yield and lower in cost and suitable for mass production and use.

The invention relates to a special composite microbial fertilizer for controlling successive cropping obstacles of radix psuedostellariae. The fertilizer is prepared by fermenting a special composite microbial inoculant and organic materials (bean pulp, rice bran, dried pig manure, fish meal and brown sugar in a weight ratio of 2:1:1:1:0.03). The composite microbial inoculant is prepared by mixing a universal beneficial bacterium solution and an aboriginal beneficial bacterium solution in a volume ratio of 4:6. The aboriginal beneficial bacterium comprises an aboriginal beneficial bacterium composite bacterium solution which is formed by independently fermenting Burkholderia, pseudomonad and Bacillus separated to the radix psuedostellariae rhizosphere soil, and mixing in a volume ratio of 4:1:1. The organic materials are subjected to special composite microbial inoculant anaerobic fermentation and oxygen-consuming fermentation to prepare the special composite microbial fertilizer. The long-term on-site verification proves that the microbial fertilizer has the advantages of reasonable formula, stable effects, favorable biocontrol effect and no pollution, has obvious effects on controlling succession cropping obstacles of radix psuedostellariae, is safe and ecological, and thus, has wide development and application prospects.

The invention provides a garden waste degradation bacteria agent. The garden waste degradation bacteria agent comprises a bacteria groupand a basic culture solution, wherein the bacteria group comprises Promicromonospora, Burkholderia, Thermomyces lanuginosus, Shigella and Streptomyces; the basic culture solution comprises wheat bran, starch, xylose, beef extract, ammonium nitrate and sterile water; the weight ratio of the bacteria group to the basic culture solution is 1:1.5-1:2. The garden waste degradation bacteria agent has the advantages that after fermentation of garden wastes is finished by using the degradation bacteria agent, the degradation rates of hemicellulose, cellulose and lignin are obviously higher than those obtained after one kind of bacteria is individually used; the Shigella and the Streptomyces are degraded by adding sugar, other polysaccharides in the garden wastes can be efficiently degraded, and the overall fermentation process is improved. The compound degradation bacteria agent is high in lignin degradation efficiency; land resources are saved, soil and the ecological environment are improved, environmental pollution is mitigated, the substrate obtained after degradation can be turned into treasure, and resource recycling is achieved.