153 results about "Burkholderia" patented technology

The invention relates to a preparation method of a novel biological soil remediation agent. The method comprises the following steps: (1) compounding and mixing four bacteria, including anaerobic spore-bearing bacilli TIBCC1.002, micrococcus luteus TCCC23001, pseudomonas fluorescens AS1.867, Burkholderia cepacia NKCCMRNK.T2-2 according to the weight part ratio of (3-6) to (2-5) to (1-4) to (1-3) to form a compound bacterium solution, adding the compound bacterium solution according to the weight ratio of 1%-5% into active sludge, and biologically enhancing the active sludge; (2) filtering biogas slurry by a hollow fiber membrane with the pore diameter of 0.1-0.2 micron so as to obtain waste biogas slurry residues; (3) mixing the biologically enhanced active sludge with the waste biogas slurry residues according to the weight part ratio of (3-9) to (1-5), and stirring uniformly to form the novel biological soil remediation agent. The invention provides another approach for the recycling of the residual sludge and biogas slurry, so that not only can the polluted soil be remediated effectively, but also the load to the environment pollution by the residual sludge and the waste biogas slurry residues can be relieved to a certain extent; the preparation method has relatively high practical application value.

The invention discloses a whole-course biological control method of tobacco diseases and insect pests. The method comprises the following steps: 1) in the seedling stage: using predatory mites for controlling thrips and tomato spotted wilf viruses; using harmonia axyridis and aphidius gifuensis for controlling myzus persicae and the thrips; using penicillium to inactivate mycelia, and 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling tobacco mosaic diseases; 2) in the field period: using the predatory mites and harmonia axyridis for controlling the thrips, the tomato spotted wilf viruses and the myzus persicae; using sex attractants for controlling prodenia litura adults and helicoverpa assulta adults; using Cahtheconidea furcellata for controlling prodenia litura larvae; using 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling the tobacco mosaic diseases; using Holder Burkholderia, and Pythium oligandrum for controlling tobacco black shank, black root rot, root knot nematode diseases and the like. The whole-course biological control method of the tobacco diseases and insect pests disclosed by the invention thoroughly eliminates pollution of chemical pesticides on the environment and tobacco pesticide residues and effectively controls the diseases and insect pests continuously and effectively, and the loss of tobacco quality for the diseases and insect pests is controlled within 5%.

The invention relates to a preparation method for burkholderia cepacia, and an application in high-performance phosphate-dissolving microbial fertilizer by using the same. The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. The phosphate-dissolving microbial fertilizer provided by the present invention has advantages of low cost, good effect, disease resistance of plants enhancing, no pollution on environment and fully utilization of potential phosphorus resource.