362 results about "Thiobacillus" patented technology

The invention relates to sulfur oxide sulfur bacilli and biological eliminating method of chromium in tanning mud. Bacterial strain is named as TS6, preserve mark is CGMCCNO.0759. The method of eliminate chromium in tanning mud by above mentioned sulfur oxide sulfur bacilli is adding 1-3 g/L sulfur into tanning mud in biology reactor, inocualting 10-15% (v/v, the same below) TS6 bacteria strain, stirring, ventilating and subsiding; that 10-20% of subsiding mud backflow to reactor; dehydrating suprplus subsiding mud; adding basic material into liquid part to turn Cr3+ into Cr(OH)3 and subside, sediment is dissolved by sulfuric acid, then there is the tannage stage; solid part is neutralized to agriculture. The elimianting rate of Cr in mud is 95%-100%, reserving rate of nutrient in mud is above 80%.

The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium/micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).

The invention relates to a microbial agent used for producing a soil modifying agent by fermentation, consisting of fiber monad, bacillus, lactobacillus, methanobacteria, thiobacillus, streptomycete, high-temperature actinomyces, high-temperature monad, azotobacteria, nitration monad, nitration bacilus, rhizobium, koji mold, leaven, blue mold, detritus mold, rhizopus as well as mycorrhizal fungi and substrate. In addition, the invention also relates to a soil modifying agent produced by the fermentation of the microbial agent, application thereof and the like.

The invention relates to a biological leaching, solvent extraction and electrowinning recovery method for heavy metal copper in sludge, and is a method of dissolving the copper from the sludge by biological leaching method, separating and enriching the copper in leaching solution by solvent extraction method, and recovering the metal coppe by electrowinning electroless plating copper technology, and is a harmless and resourceful treatment technology for municipal sludge and industrial sludge with higher copper content. Firstly, the biological acidization which lowers the ph value of a sludge system and oxygenizement of compound acidophilic thiobacillus under aerobic condition in the presence of energy matter are utilized to enable the heavy metal copper in the sludge to abundantly dissolved out from solid phase to liquid phase, so that the sludge can be harmless; secondly, the copper in the biological leaching solution is extracted by using a copper extractant M5640 so that the copper enters an organic phase, and then the copper is conducted back extractioin by using sulfuric acid so that the copper enters the water phase again so as to separate and enrich the copper; finally, the enriched copper solution is electrolyzed to causes the copper to be deposited, and the copper is recovered to be resourceful. The technique not only can remove the heavy metals in the sludge and kill pathogens, but also ensures that the treated sludge is easy to dehydrate and settle and the heavy metal copper dissolved out can be recovered to achieve the purposes of harmlessness, reduction and resource of the sludge.

This invention relates to improved methods, processes and apparatus for the removal of sulfides from a gas, liquid or solid (substance) wherein the substance is contacted with an aqueous solution. The instant invention presents methods and processes wherein at least one of H₂S, SO₂ and CS₂ is chemically converted in an aqueous media to a salt and/or compound comprising sulfur and a cationic moiety. Said salt and/or compound comprising sulfur and a cationic moiety is herein termed a “Sulfur Salt”. After formation of the Sulfur Salt, the Sulfur Salt is converted to elemental sulfur with a bacterium capable of metabolizing sulfur. The preferred bacterium for metabolizing sulfur is a strain from the genus Thiobacillus. The most preferred strain from the genus Thiobacillus is Thiobacillus denitrificans. The instant invention prefers an aqueous operating pH of between 6.0 and 8.0, while the most preferred aqueous pH is between 6.0 and 7.0.

The invention relates to a method to extract copper from abandoned breadboards and to use remain substance comprehensively recycling by combining techniques of microbe, metallurgy and materials. Based on principles of microbial wet-process metallurgy, Cu is extracted from abandoned breadboards by using iron protoxide thiobacillus. At the same time, the retained solid is recycled to realize the comprehensive treatment of abandoned breadboards. The entire recycled treatment of abandoned breadboards is realized, which guarantees the entirely clean produce without discharging waste water, waster residue and waste gas into the environment. This method has much strongpoint including little investment, low cost, high recovery rate of metal, non-pollution and green zoology etc. It benefits the society, economy and environment.

The invention relates to a compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate and a preparation method thereof. The use amount of calcium nitrate is reduced, and the time for treating the black and odorous rivers is shortened. The compound microbial preparation is prepared from 40-80 parts of thiobacillus denitrificans and thiocapsa roseoppersicina mixed powder, 10-60 parts of composite bacillus subtilis powder and 10-60 parts of yeast and lactic acid bacterium mixed powder. By means of the preparation, sulfide in black and odorous bottom sludge, organic matter and strains suitable for growing in the black and odorous bottom sludge are screened and degraded in a targeted mode; by means of thiobacillus denitrificans with efficient hydrogen sulfide degrading and denitrification capacity, sulfur-oxidizing bacteria of thiocapsa roseoppersicina with efficient hydrogen sulfide and ammoniacal nitrogen degrading capacity, bacilli rich in protease, amylase, cellulose and other enzyme systems, yeast and lactic acid bacteria, a small amount of calcium nitrate and the compound microbial preparation are thrown into the black and odorous rivers so that water quality of the black and odorous rivers can be quickly improved, the black and odorous bottom sludge can be removed quickly, and the thickness and the organic matter content of the bottom sludge can be quickly reduced.

The invention discloses a method for synchronously removing nitrogen and phosphorus in sewage containing nitrogen and phosphorus, belonging to the field of sewage treatment. The method comprises the steps of (1) screening thiobacillus denitrificans: screening thiobacillus denitrificans from anaerobic activated sludge and taking as target strain, wherein thiobacillus denitrificans utilizes sulphur source in ferrous sulfide to conduct autotrophic denitrification; (2) preparing fillers of a reactor: directly adding ferrous sulfide with the particle size smaller than 25mm in a reaction container; (3) adding sewage: feeding sewage to be treated into the reaction container, wherein the sewage to be treated is the sewage containing nitrogen and phosphorus with the pH value of 5.0-9.0; (4) conducting the autotrophic denitrification process: conducting the mixed reaction for 2 to 6d at the temperature of 10 DEG C-40 DEG C under the anaerobic condition in the reaction container, reducing NO2<-> and NO3<-> in water to be N2 by thiobacillus denitrificans while simultaneously oxidizing ferrous sulfide by thiobacillus denitrificans, and simultaneously, precipitating phosphorus in water by utilizing iron ions (being oxidation products); and (5) separating solid and liquid. According to the method, the technology for removing nitrogen by utilizing thiobacillus denitrificans and the technology for removing phosphorus by using iron ions are fused naturally, the reaction cost is low, and the processing effect is good.

The invention discloses a method for recycling heavy metal in electroplating sludge. The method comprises the following steps of: carrying out screening and acclimatizing to obtain thelloobacillus ferrooxidans and thiobacillus thiooxidans which have resistance of high-concentration heavy metal; under a certain condition, respectively stirring and mixing the thelloobacillus ferrooxidans and the thiobacillus thiooxidans with the electroplating sludge together; biologically leaching the mixture so as to obtain copper-contained leach liquor or nickel-contained leach liquor; and carrying out electrodeposition on the copper-contained leach liquor after removal of impurities, so as to recycle copper, and carrying out the electrodeposition on the nickel-contained leach liquor after the removal ofthe impurities, so as to recycle nickel.

An acid-resistant rhodotorula R30 (CGMCC No.1147) for removing heavy metals from sludge is disclosed. The process for removing heavy metals from sludge includes loading the sludge in bioreactor, inoculating said rhodotorula R30 and thiobacillus TS6, aerating at 28 deg.C for 2-6 days, dewatering of sludge, regulating pH of liquid phase for depositing heavy metals, and neutralizing solid phase for agricultural purpose.

The invention discloses a saline-alkali soil biological improved fertilizer based on high-sulfur gangue. The saline-alkali soil biological improved fertilizer is prepared from the following components in parts by weight: 50-60 parts of high-sulfur gangue microbial fermentation product, 20-30 parts of compost cow manure or chicken manure, 1-2 parts of citric acid, 1-2 parts of oxalic acid, 2-4 parts of urea, 2-4 parts of ammonium sulfate, 2-4 parts of monoammonium phosphate, 1-3 parts of potassium sulphate, 2-5 parts of ferrous sulfate, 1-2 parts of zinc sulfate, 1-2 parts of manganese sulfate and 1-10 parts of bentonite, wherein the high-sulfur gangue microbial fermentation product is a product obtained by performing activating treatment on the high-sulfur gangue by a compound microorganism bacterium solution composed of thiobacillus thiooxidans, bacillus subtilis, bacillus megatherium, bacillus mucilaginosus and enterococcus faecalis. The resource utilization of the high-sulfur gangue is realized, the improvement of the inland saline land can be promoted, the crop yield can be increased and the entironment can be improved.

The invention discloses an efficient biological desulfurization method for coal, which is characterized in that coal is crushed through a crusher until the particle size is below 150 meshes; and three bacteria, namely Thiobacillus thioxidans, Thiobacillus caldus and Pseudomonas stutzeri, are adopted. The desulfurization process comprises the following sequential steps: removing organic sulfur in the coal through the Pseudomonas stutzeri; after the primary desulfurization is finished, preparing the Thiobacillus thioxidans and the Thiobacillus caldus into a desulfurization solution according to a certain total bacterial count ratio, and removing inorganic sulfur of the coal; and washing the desulfurized coal with water, filtering, and drying. The total sulfur removal ratio of the desulfurized coal is up to 57.8%; and the method is simple to operate, mild in treatment conditions and low in cost, and effectively retains effective components in the coal, thus being a desulfurization technology having very wide market prospects.

The invention relates to a low carbon nitrogen ratio micro contaminated water nitrogen removal method. A sulphur/limestone/forest tree waste composite nitrogen removal filling material is layered and placed in a up flow reactor, and anaerobic sludge is inoculated into the filling material; HRT is set and started for 8 hours, and raw water is pumped, and a thiobacillus denitrificans medium is continuously let in for dynamic culture domestication of sludge, until stable operation; after ten days of operation, Na2S2O3.5H2O in the thiobacillus denitrificans medium is reduced to a half, and domestication is continued until a biomembrane grows mature again, and then Na2S2O3.5H2O in the thiobacillus denitrificans medium is reduced to zero, thereby microorganisms can utilize the sulphur in the filling material as sulfur source for growth and breeding; after ten days of system operation, a stable biomembrane is finally formed, and a nitrogen removal treatment of normal micro pollution water is carried out. A forest tree waste is added in the filling material according to the invention, and heterotrophic denitrifying bacterium are increased, and a mixed nutrition type nitrogen removal system is formed, and the invention has the advantages of short domestication time, good anti-interference capability, good nitrogen removal effect without secondary pollution.

The invention relates to an ore extraction technology, in particular to a method for leaching low-grade phosphorite with mixed bacillus, wherein the mixed bacillus consists of a thiobacillus thioxidans At.t which is separated from soil sample of the Hubei tongshankou copper mine and a thiobacillus ferrooxidans At.f which is separated from acid water hole of the Hubei tonglushan copper mine. The method comprises the following steps of: 1) sampling from the soil sample of the Hubei tongshankou copper mine and the acid water hole of the Hubei tonglushan copper mine; 2) separating the thiobacillus thioxidans and the thiobacillus ferrooxidans in an enrichment way and domesticating; and 3) leading the phosphorus. The method has the beneficial effects of: 1. being high in the activity of mixed bacillus: the phosphorus leading rate is high up to 88.75%; 2. being low in cost: the cost of the 10% inoculum size is far lower than that of the 20%-30% inoculum size of the thiobacillus thiooxidans in the prior art, so that the cost for preparing culture solution with plenty of expensive medicaments is reduced; 3. being short in period and high in efficiency; and 4. being environment-friendly: the usage amount of the acid bacillus is small, thereby being good for reducing environment pollution.

The invention discloses compound bactericide for treating sewage and a sewage treatment method thereof, and belongs to the technical field of sewage treatment. The compound bactericide comprises the following active ingredients from following strains: rhodopseudomonas, anaerobic ammonium oxidation bacteria, candida utilis, thiobacillus denitrificans, white-rot fungi, lactobacillus plantarum, bifidobacterium, lactobacillus acidophilus, green nonsulfur bacteria, phosphorus-accumulating bacteria, aspergillus oryzae, nitrosomas, streptomyces, bacillus subtilis and propionibacterium acidipropionici. The invention also provides the method using the compound bactericide to treat the sewage. The compound bactericide and the sewage treatment method have the advantages that the effect of treating sewage is better, the technology is simple, the implementing is easy, the operability is strong, the pollution to environment is avoided, the industrial requirements are met, and the application prospect is better.

The invention discloses a method for deeply treating sewage by using calcined pyrite as a filtering material. The method is characterized in that calcined pyrite is adopted as the filtering material. Anaerobic microbes such as denitrifying bacteria, thiobacillus denitrificans, iron-oxidizing bacteria depending on nitrate, ammonia-oxidizing bacteria, and the like are loaded on the surface of the filtering material. Second-stage treatment discharged water from a domestic sewage treatment factory, eutrophic ground surface water, or aquaculture water is continuously delivered to a filtering tank loaded with calcined pyrite for treatment, until phosphorous concentration of discharged water exceeds 0.1mg/L. With the method provided by the invention, a comprehensive effect of synchronous nitrogen removing, phosphorous removing, and organic pollutant concentration reducing is provided. The treatment efficiency is high.

The invention discloses a biological deodorant. The biological deodorant is characterized by comprising the following raw materials in percentage by mass: 20%-30% of a complex microbial agent, 40%-50% of organic acid and 30%-40% of calcium superphosphate, wherein the complex microbial agent comprises saccharomyces cerevisiae, bacillus subtilis, germany lactic acid bacteria, nitrobacteria, thiobacillus thiooxidans, acetobacter oxydans and an adsorption material; and the organic acid is selected from one or more of oleic acid, linoleic acid, linolenic acid, arachidonic acid, polybasic carboxylic acid, oxalic acid, malic acid, amino acid, sulfoacid and sulfinic acid. According to the biological deodorant, the original foul gas in garbage can be quickly and effectively adsorbed, removed and covered, the work environment of a worker in a composting plant is improved, composting and stabilizing of organic garbage are accelerated, and novel foul gases such as ammonia and hydrogen sulfide generated and released in the composting process are prevented from being reduced.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

A process for desulfurizing iron ore concentrate includes such steps as culturing the mixture of thiobacillus ferro oxidans and thiobacillus sulfur oxidans in the non-Fe culture medium containing sulfur powder as energy source, adding the high-S iron ore concentrate containing pyrrhotite, naturalizing culture to obtain desulfurizing bacteria, putting them in water, immersing said high-S iron ore concentrate in it, vibrating in shaker for desulfurizing, washing and drying.