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Thiobacillus is a genus of Gram-negative Betaproteobacteria. Thiobacilus thioparus is the type species of the genus, and the type strain thereof is the Starkeyᵀ strain, isolated by Robert Starkey in the 1930s from a field at Rutgers University in the United States of America. While over 30 "species" have been named in this genus since it was defined by Martinus Beijerinck in 1904, (the first strain was observed by the biological oceanographer Alexander Nathansohn in 1902 - likely what we would now call Halothiobacillus neapolitanus), most names were never validly or effectively published. The remainder were either reclassified into Paracoccus, Starkeya (both in the Alphaproteobacteria); Sulfuriferula, Annwoodia, Thiomonas (in the Betaproteobacteria); Halothiobacillus, Guyparkeria (in the Gammaproteobacteria), or Thermithiobacillus or Acidithiobacillus (in the Acidithiobacillia). The very loosely defined "species" Thiobacillus trautweinii was where sulfur oxidising heterotrophs and chemolithoheterotrophs were assigned in the 1910-1960s era, most of which were probably Pseudomonas species. Many species named in this genus were never deposited in service collections and have been lost.
An autotrophic denitrification bacterial agent and a preparation method thereof
This invention relates to the field of environmental microbiology, specifically disclosing an autotrophic denitrifying bacterial agent and its preparation method. This autotrophic denitrifying bacterial agent employs a three-layer biomimetic design: a sulfur-calcium composite core, an inner shell gel layer, and an outer functional layer. Denitrifying Thiobacillus is loaded into the core, and Pseudomonas schlegelii is fixed in the outer layer, with a sodium alginate-xanthan gum gel layer providing physical isolation, achieving spatial partitioning and symbiosis of autotrophic and heterotrophic denitrifying bacterial communities. The sulfur-calcium composite core uses sulfur powder as an electron donor, and hydrophobically modified eggshell powder supplements alkalinity in situ. The inner shell layer incorporates ultrafine calcium carbonate, providing both mechanical reinforcement and pH buffering functions. The outer layer encapsulates sodium acetate-stearate microspheres for slow carbon source release. This autotrophic denitrifying bacterial agent exhibits high denitrification efficiency, pH stability, and strong resistance to hydraulic shearing under low carbon-to-nitrogen ratio conditions. The preparation process is mild, and the raw materials are inexpensive and readily available, making it suitable for deep denitrification in wastewater treatment and other scenarios.
Owner:GUANGZHOU WEICHUAN BIOTECHNOLOGY CO LTD
Method for advanced nitrogen removal of secondary effluent of sewage plant
ActiveCN119430501BReduce processing costsMeet the requirements of deep denitrificationWater contaminantsBiological water/sewage treatmentBiofilmMicroorganism
The present application relates to the technical field of environmental pollution treatment, in particular to a method for advanced denitrification of secondary effluent of sewage plant, comprising the following steps: S1. taking volcanic rock and sulfur particles as the filler for biofilm adhesion, starting the biofilter column, and after successful start, the functional microorganisms in the biofilm include Thiobacillus and Candidatus Kuenenia; S2. mixing the primary sedimentation tank effluent and the secondary effluent according to the volume ratio of (4.5-y) / (x-4.5)~(5.5-y) / (x-5.5) to obtain the mixed liquor, wherein x and y are the ammonia nitrogen concentrations in the primary sedimentation tank effluent and the secondary effluent respectively, and the unit is mg / L; S3. taking the mixed liquor as the water to be treated for denitrification treatment, and monitoring the water quality of the treated effluent; S4. judging whether to adjust the treatment process according to the nitrogen content in the effluent. The present method can realize the advanced denitrification of the secondary effluent of sewage plant, and has low treatment cost.
Owner:ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
A method for culturing thiobacillus ferroxidans
PendingCN122104478ABioreactor/fermenter combinationsBiological substance pretreatmentsMicroorganismMicrobial agent
The application provides a culture system and culture method of Thiobacillus ferroxidans, wherein the system comprises a nutrient solution tank, a culture tank, a communication device, an overflow separator, a clear liquid tank, a sedimentation tank and a reflux device, the overflow separator is provided with a solid discharge control valve at the bottom, the precipitate is discharged from the solid discharge control valve, and the overflow liquid returns to the culture tank, so that the precipitate and the Thiobacillus ferroxidans are separated; the culture method realizes the efficient culture of the Thiobacillus ferroxidans through the steps of preparing a nutrient solution, inoculation, aeration culture, circulating flow addition, precipitation separation and continuous culture, solves the problem that the precipitate such as iron alum affects the growth of microorganisms in the culture process, guarantees the fresh culture liquid supply, promotes the continuous propagation and growth of the microorganisms, and thus improves the output efficiency and the living quality of the Thiobacillus ferroxidans. The obtained microbial suspension can be prepared into a microbial agent product and can also be directly used in the fields of bio-metallurgy, environmental remediation, bio-electrochemistry and bio-fuel cells.
Owner:CHANGSHA AIRUI CONSULTING SERVICES CO LTD
Bacterial microcompartment virus-like particles
ActiveUS12667614B2Virus-like particleThiobacillus
The present invention relates to a method for producing a bacterial microcompartment virus-like particle (VLP) carrying a cargo molecule, the method comprising introducing and expressing in a host cell or organism one or more polynucleotides comprising (a) a first sequence encoding bacterial microcompartment shell protomers and a second sequence encoding a cargo molecule fused to an encapsulation peptide comprising the sequence SKITGSSGNDTQGSLITYSGGARG, and forming a microcompartment that encapsulates the cargo molecule, or (b) a first sequence encoding bacterial microcompartment shell protomers and a second sequence encoding at least one of said protomers fused with a cargo molecule or a biochemical tag, and forming a microcompartment that expresses the cargo molecule or biochemical tag on an exterior surface. In one embodiment, the bacterial microcompartment protomers are CsoSIA and CsoS4A from Halothiobacillus neapolitanus, or HO-H, HO-P and HO-T1 from Haliangium ochraceum.
Owner:NATIONAL UNIVERSITY OF SINGAPORE
A composite microbial agent, a preparation method and application thereof
PendingCN122278669AOvercome limitations of deficienciesEfficient removalBiotechnologyHaloxylon ammodendron
This invention belongs to the field of biological wastewater treatment, specifically relating to a composite microbial agent, its preparation method, and its application. By mass percentage, it comprises 60%-80% bioactive agent and 20%-40% activity protectant. The bioactive agent includes 66.58%-86.58% of *Haloxylon ammodendron* strains, 7.85%-22.85% of *Hymenobacter* strains, and 5.57%-25.57% of *Thiobacillus* strains. The total viable count of the composite microbial agent is not less than 1 × 10⁻⁶. 9 The compound microbial agent was added to distilled water and cultured under anaerobic shaking conditions to obtain a revived bacterial solution. The supernatant of the revived bacterial solution was mixed with the wastewater to be treated at a volume ratio of 1-10:250, and 100-250 mg / L of a compatible solute was added. The wastewater was then treated under anaerobic conditions. After one week of treatment of low C / N ratio, high-salt phenol wastewater using this compound microbial agent, the denitrification rate remained above 95%, and the phenol degradation rate remained above 80%. This effectively solved the problems of low denitrification efficiency and difficulty in removing phenolic compounds from high-salt phenol wastewater, and the process was simple and low-cost.
Owner:TIANJIN CHENGJIAN UNIV
A method for extracting manganese and silver from oxidized manganese silver ore and acidophilic thiobacillus
The application provides a method for extracting manganese and silver from oxidized manganese silver ore and acidophilic Thiobacillus, and the method comprises the following steps: step 1, crushing and finely grinding the oxidized manganese silver ore and reducing minerals, and then making a slurry to obtain a mineral slurry; step 2, adding sulfuric acid into the mineral slurry and performing chemical leaching, and then performing solid-liquid separation on the obtained chemical leaching product to obtain a chemical leaching residue and a manganese-rich low-acid chemical leaching liquid; step 3, inoculating the chemical leaching residue with the acidophilic Thiobacillus and then performing biological leaching, and then performing solid-liquid separation on the obtained biological leaching product to obtain a low-manganese high-acid biological leaching liquid and a silver-containing biological leaching residue; and step 4, leaching silver from the silver-containing biological leaching residue. The method provided by the application is a full-wet extraction process, and is particularly suitable for treating low-grade oxidized manganese silver ore, can help to reduce the mining boundary grade of manganese silver resources, can improve the resource utilization efficiency, and can obtain greater economic benefits, social benefits and environmental benefits.
Owner:GRINM RESOURCES & ENVIRONMENT TECH CO LTD