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22 results about "Thiobacillus" patented technology

Thiobacillus is a genus of Gram-negative Betaproteobacteria. Thiobacilus thioparus is the type species of the genus, and the type strain thereof is the Starkeyᵀ strain, isolated by Robert Starkey in the 1930s from a field at Rutgers University in the United States of America. While over 30 "species" have been named in this genus since it was defined by Martinus Beijerinck in 1904, (the first strain was observed by the biological oceanographer Alexander Nathansohn in 1902 - likely what we would now call Halothiobacillus neapolitanus), most names were never validly or effectively published. The remainder were either reclassified into Paracoccus, Starkeya (both in the Alphaproteobacteria); Sulfuriferula, Annwoodia, Thiomonas (in the Betaproteobacteria); Halothiobacillus, Guyparkeria (in the Gammaproteobacteria), or Thermithiobacillus or Acidithiobacillus (in the Acidithiobacillia). The very loosely defined "species" Thiobacillus trautweinii was where sulfur oxidising heterotrophs and chemolithoheterotrophs were assigned in the 1910-1960s era, most of which were probably Pseudomonas species. Many species named in this genus were never deposited in service collections and have been lost.

Ferrous oxide recombinant bacteria, recombination method and application of ferrous oxide recombinant bacteria in lithium battery metal recovery

The invention belongs to the field of genetic engineering and resource recycling, and relates to a ferrous oxide recombinant bacterium, a recombination method and application of the ferrous oxide recombinant bacterium in lithium battery metal recovery. The ferrous oxide recombinant bacterium is obtained by taking thiobacillus ferrooxidans as an original strain through genetic engineering modification; comprising thiobacillus ferrooxidans, an exogenously introduced nickel / cobalt efflux protein RcnA gene, a vector pJRD215 and donor bacteria, and the nickel / cobalt efflux protein RcnA gene is introduced into the vector pJRD215, driven to express and then introduced into the donor bacteria to express. The application of the constructed and prepared ferrous oxide recombinant bacteria in lithium battery metal recovery is used for improving the leaching efficiency of metal ions Co < + > or / and Li < + > or / and Ni < + > in lithium battery powder; and the method shows good adaptability and stability under different substrate concentrations, and provides a key experimental support and an important experimental basis for application of a microbial enhanced leaching technology in the field of waste lithium battery resource utilization.
Owner:CHONGQING UNIV

La and Ce toxicity resistant thiobacillus thiooxidans and application thereof

PendingCN121160509ABacteriaTransportation and packagingThiobacillus ferrooxidansThiobacillus
The invention relates to the technical field of solid waste biological treatment, and discloses La and Ce toxicity resistant thiobacillus thiooxidans and application thereof, and the collection number of the thiobacillus thiooxidans is GDMCC No: 63522. The invention provides a novel thiobacillus thiooxidans, and the preservation number of the novel thiobacillus thiooxidans is GDMCC No: 63522. The strain can highly tolerate La and Ce, and can realize biological leaching of solid wastes rich in La or (and) Ce under high ore pulp density, so that more solid wastes can be treated in a single batch, the biological leaching rate is improved, and the strain has a good application prospect.
Owner:CHINA PETROLEUM & CHEMICAL CORP +1

Metal leaching agent and method for leaching and recovering metal from retired photovoltaic module step by step

PendingCN121320747AProcess efficiency improvementThiobacillus ferrooxidansMetal leaching
The invention relates to a metal leaching agent and a method for leaching and recovering metal from a retired photovoltaic module step by step. The metal leaching agent comprises an acidophilic thiobacillus ferrooxidans solution, a hidden acidophilic bacteria solution and a thiobacillus thiooxidans solution, the volume ratio of the acidophilic thiobacillus ferrooxidans liquid to the hidden acidophilic bacteria liquid to the thiobacillus thiooxidans liquid is (0.5-1.5): (0.5-1.5): (0.5-1.5); wherein the concentration of the acidophilic thiobacillus ferrooxidans, the acidophilic hidden bacteria and the thiobacillus thiooxidans is (1-9) * 10 < 8 > cells / mL. Furthermore, the invention provides a method for leaching and recovering metal from the retired photovoltaic module step by step. According to the method, the metal leaching agent is utilized, mild and green recovery of aluminum and silver in the decommissioned photovoltaic cell piece and full-process recovery of the decommissioned photovoltaic module can be achieved through step-by-step biological leaching, and the method has the excellent leaching effect on aluminum and silver.
Owner:SUN YAT SEN UNIV

Repair method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.
Owner:HUBEI UNIV

Cross-feeding composite microbial agent for simultaneous removal of beneficiation reagents and heavy metal pollution and application thereof

The application of the cross-feeding composite microbial agent for simultaneously removing mineral processing reagents and heavy metal pollution belongs to environmental microbial remediation and wastewater treatment technology, and is a mixture of a sulfur-oxidizing bacteria (Thiobacillus.sp ) bacterial suspension and a sulfate-reducing bacteria Desulfotomaculum_profundi ) bacterial suspension. The cross-feeding composite microbial agent is obtained by inoculating the mixed bacterial liquid into a liquid culture medium and culturing at 30 DEG C under constant temperature and micro-oxygen conditions at 120 rpm until the OD600 value reaches 0.6-1.0. The cross-feeding composite microbial agent realizes simultaneous xanthate high-efficiency degradation and heavy metal (such as Cu²⁺, Pb²⁺ and Zn²⁺) simultaneous fixation treatment in one reactor through the metabolic synergistic effect of the sulfur-oxidizing bacteria Thiobacillus.sp ) and the sulfate-reducing bacteria Desulfotomaculum profundi ).
Owner:SOUTH CHINA NORMAL UNIV

An autotrophic denitrification bacterial agent and a preparation method thereof

PendingCN122357518ASodium acetateElectron donor
This invention relates to the field of environmental microbiology, specifically disclosing an autotrophic denitrifying bacterial agent and its preparation method. This autotrophic denitrifying bacterial agent employs a three-layer biomimetic design: a sulfur-calcium composite core, an inner shell gel layer, and an outer functional layer. Denitrifying Thiobacillus is loaded into the core, and Pseudomonas schlegelii is fixed in the outer layer, with a sodium alginate-xanthan gum gel layer providing physical isolation, achieving spatial partitioning and symbiosis of autotrophic and heterotrophic denitrifying bacterial communities. The sulfur-calcium composite core uses sulfur powder as an electron donor, and hydrophobically modified eggshell powder supplements alkalinity in situ. The inner shell layer incorporates ultrafine calcium carbonate, providing both mechanical reinforcement and pH buffering functions. The outer layer encapsulates sodium acetate-stearate microspheres for slow carbon source release. This autotrophic denitrifying bacterial agent exhibits high denitrification efficiency, pH stability, and strong resistance to hydraulic shearing under low carbon-to-nitrogen ratio conditions. The preparation process is mild, and the raw materials are inexpensive and readily available, making it suitable for deep denitrification in wastewater treatment and other scenarios.
Owner:GUANGZHOU WEICHUAN BIOTECHNOLOGY CO LTD

Method for efficiently leaching nickel in wet treatment tailings of waste power battery positive electrode material

PendingCN121344376AProcess efficiency improvementThiobacillus ferrooxidansPower battery
The invention discloses a method for efficiently leaching nickel in waste power battery positive electrode material wet treatment tailings, which comprises the following steps: collecting the tailings generated by waste power battery positive electrode material wet treatment, and pretreating the tailings to obtain nickel slag; and the mixed bacterial liquid is put into an improved culture medium to form a leaching system, the pH value of the leaching system is adjusted to 1.8-2.2, the temperature of the leaching system is adjusted to 40-45 DEG C, then the nickel slag is added for leaching treatment, and a nickel-rich solution and leaching slag with the nickel content smaller than or equal to 0.1% are obtained through leaching. According to the method, the nickel slag is efficiently leached by using the mixed bacterial liquid compounded by thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans, thiobacillus caldus and temperature-resistant thiobacillus oxydans, nickel can be fully recycled, and the problems of waste of nickel resources and potential environmental pollution of nickel slag treatment are avoided.
Owner:NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA +1

Recovery method for microorganism-assisted electrochemical flotation mixed copper-cobalt ore

The invention provides a brand-new efficient recovery method for mixed copper-cobalt ore, which comprises the following steps: firstly, crushing and desliming, then inoculating thiobacillus ferrooxidans in a culture medium in which nano iron oxide is pre-dispersed, culturing, and finally adopting an electrochemical flotation system with an anode as a titanium-based platinum-plated electrode and a cathode as a foamed nickel electrode, and flotation is performed in three stages under the control of a pulse power supply. According to the method, a microorganism-assisted electrochemical flotation technology and a nanometer material modification and gradient flotation strategy are combined, the surface property of the ore is changed through microorganism metabolism, metabolism is accelerated through electrochemistry to generate bubbles, flotation selectivity is enhanced with the help of a nanometer material, and finally efficient recovery of the mixed copper-cobalt ore is achieved.
Owner:CENT SOUTH UNIV

Culture medium for rapidly culturing thiobacillus denitrificans as well as preparation method and application of culture medium

The invention discloses a culture medium for rapidly culturing thiobacillus denitrificans as well as a preparation method and application of the culture medium, and belongs to the technical field of microbial culture. Aiming at the chemical energy autotrophic metabolism characteristics of thiobacillus denitrificans, a highly adaptive, stable and efficient growth environment is cooperatively constructed through precise formula regulation and optimized step-by-step preparation and classified sterilization processes. The culture medium disclosed by the invention can remarkably shorten the growth delay phase of thalli, promote the thalli to quickly enter the logarithmic phase and realize high-density and high-activity stable culture, and the cultured thiobacillus denitrificans show excellent denitrification performance in sewage treatment. The culture medium provided by the invention systematically solves the core problems of long culture period, low efficiency, high cost, poor large-scale stability and the like in the prior art, and has a good application prospect.
Owner:ZHONGYI ENVIRONMENTAL BIOTECHNOLOGY (JIANGSU) CO LTD +3

Acidithiobacillus thiooxidans and application thereof

The invention provides acidophilic thiobacillus thiooxidans and application thereof, and belongs to the technical field of acidophilic thiobacillus thiooxidans. The acidophilic thiobacillus thiooxidans is named as HS-PLS23-At.t, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34267. The acidophilic thiobacillus thiooxidans is named as HS-PLS23-At.t. Research finds that the acidophilic thiobacillus thiooxidans can grow well when the initial pH is 1-6, and has strong acid resistance. Meanwhile, the acidophilic thiobacillus thiooxidans can generate sulfuric acid from elemental sulfur, so that the acidophilic thiobacillus thiooxidans can efficiently produce sulfuric acid and can also promote leaching of uranium and iron in uranium ores.
Owner:EAST CHINA UNIV OF TECH

Method for advanced nitrogen removal of secondary effluent of sewage plant

ActiveCN119430501BReduce processing costsMeet the requirements of deep denitrificationWater contaminantsBiological water/sewage treatmentBiofilmMicroorganism
The present application relates to the technical field of environmental pollution treatment, in particular to a method for advanced denitrification of secondary effluent of sewage plant, comprising the following steps: S1. taking volcanic rock and sulfur particles as the filler for biofilm adhesion, starting the biofilter column, and after successful start, the functional microorganisms in the biofilm include Thiobacillus and Candidatus Kuenenia; S2. mixing the primary sedimentation tank effluent and the secondary effluent according to the volume ratio of (4.5-y) / (x-4.5)~(5.5-y) / (x-5.5) to obtain the mixed liquor, wherein x and y are the ammonia nitrogen concentrations in the primary sedimentation tank effluent and the secondary effluent respectively, and the unit is mg / L; S3. taking the mixed liquor as the water to be treated for denitrification treatment, and monitoring the water quality of the treated effluent; S4. judging whether to adjust the treatment process according to the nitrogen content in the effluent. The present method can realize the advanced denitrification of the secondary effluent of sewage plant, and has low treatment cost.
Owner:ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY

A method for culturing thiobacillus ferroxidans

The application provides a culture system and culture method of Thiobacillus ferroxidans, wherein the system comprises a nutrient solution tank, a culture tank, a communication device, an overflow separator, a clear liquid tank, a sedimentation tank and a reflux device, the overflow separator is provided with a solid discharge control valve at the bottom, the precipitate is discharged from the solid discharge control valve, and the overflow liquid returns to the culture tank, so that the precipitate and the Thiobacillus ferroxidans are separated; the culture method realizes the efficient culture of the Thiobacillus ferroxidans through the steps of preparing a nutrient solution, inoculation, aeration culture, circulating flow addition, precipitation separation and continuous culture, solves the problem that the precipitate such as iron alum affects the growth of microorganisms in the culture process, guarantees the fresh culture liquid supply, promotes the continuous propagation and growth of the microorganisms, and thus improves the output efficiency and the living quality of the Thiobacillus ferroxidans. The obtained microbial suspension can be prepared into a microbial agent product and can also be directly used in the fields of bio-metallurgy, environmental remediation, bio-electrochemistry and bio-fuel cells.
Owner:CHANGSHA AIRUI CONSULTING SERVICES CO LTD

A sulfur autotrophic denitrification process

PendingCN122647054ABiofilmSulfur granules
The application relates to the technical field of sewage treatment, and more specifically to a sulfur autotrophic denitrification process, which is prepared by combining sulfur-calcium carbonate eutectic granulation with PTFE microporous coating technology, and has the functions of pH self-buffering and anti-blocking; three functional zones are arranged in the reactor: the upper layer is filled with coated sulfur particles and inoculated with Thiobacillus bacteria, calcium carbonate is used to neutralize H + The coated layer controls the excessive proliferation of the biofilm, the nitrogen gas is promoted to escape under high flow rate, the middle layer of the honeycomb ceramic is inoculated with anaerobic string bacteria to degrade residual nitrite nitrogen, the flow rate is reduced to 5-8 m / h to prolong the residence time, the lower layer of the magnesium olivine cooperates with the inclined plate precipitation module to realize efficient separation of sulfate precipitation and shed biofilm under low flow rate. Through water inlet pH pre-adjustment, DO < 0.5 mg / L and gradual load domestication, the functional bacteria group can tolerate pH 5.5-8.0, and the problems of large denitrification efficiency fluctuation, nitrite nitrogen accumulation and filler blockage in the traditional process are solved, and the system realizes efficient, impact-resistant and long-term stable operation.
Owner:GUANGDONG TAIQUAN ENVIRONMENTAL PROTECTION TECH CO LTD

Bacterial microcompartment virus-like particles

ActiveUS12667614B2Virus-like particleThiobacillus
The present invention relates to a method for producing a bacterial microcompartment virus-like particle (VLP) carrying a cargo molecule, the method comprising introducing and expressing in a host cell or organism one or more polynucleotides comprising (a) a first sequence encoding bacterial microcompartment shell protomers and a second sequence encoding a cargo molecule fused to an encapsulation peptide comprising the sequence SKITGSSGNDTQGSLITYSGGARG, and forming a microcompartment that encapsulates the cargo molecule, or (b) a first sequence encoding bacterial microcompartment shell protomers and a second sequence encoding at least one of said protomers fused with a cargo molecule or a biochemical tag, and forming a microcompartment that expresses the cargo molecule or biochemical tag on an exterior surface. In one embodiment, the bacterial microcompartment protomers are CsoSIA and CsoS4A from Halothiobacillus neapolitanus, or HO-H, HO-P and HO-T1 from Haliangium ochraceum.
Owner:NATIONAL UNIVERSITY OF SINGAPORE

A composite microbial agent, a preparation method and application thereof

PendingCN122278669AOvercome limitations of deficienciesEfficient removalBiotechnologyHaloxylon ammodendron
This invention belongs to the field of biological wastewater treatment, specifically relating to a composite microbial agent, its preparation method, and its application. By mass percentage, it comprises 60%-80% bioactive agent and 20%-40% activity protectant. The bioactive agent includes 66.58%-86.58% of *Haloxylon ammodendron* strains, 7.85%-22.85% of *Hymenobacter* strains, and 5.57%-25.57% of *Thiobacillus* strains. The total viable count of the composite microbial agent is not less than 1 × 10⁻⁶. 9 The compound microbial agent was added to distilled water and cultured under anaerobic shaking conditions to obtain a revived bacterial solution. The supernatant of the revived bacterial solution was mixed with the wastewater to be treated at a volume ratio of 1-10:250, and 100-250 mg / L of a compatible solute was added. The wastewater was then treated under anaerobic conditions. After one week of treatment of low C / N ratio, high-salt phenol wastewater using this compound microbial agent, the denitrification rate remained above 95%, and the phenol degradation rate remained above 80%. This effectively solved the problems of low denitrification efficiency and difficulty in removing phenolic compounds from high-salt phenol wastewater, and the process was simple and low-cost.
Owner:TIANJIN CHENGJIAN UNIV

Thiobacillus thiooxidans resistant to toxicity of Ni and Cu and application of thiobacillus thiooxidans

PendingCN121160508ABacteriaTransportation and packagingThiobacillus ferrooxidansThiobacillus
The invention relates to the technical field of biological treatment of solid wastes, and discloses thiobacillus thiooxidans resistant to toxicity of Ni and Cu and application of the thiobacillus thiooxidans. Wherein the thiobacillus thiooxidans is the thiobacillus thiooxidans QH2C, and the preservation number of the thiobacillus thiooxidans is GDMCC No: 63521. The invention further discloses a preparation method of the thiobacillus thiooxidans. The invention provides a novel thiobacillus thiooxidans QH2C, and the strain has high toxicity tolerance to Ni and Cu, so that the strain can be used for treating metal-containing solid wastes with higher ore pulp density in batches, the biological leaching treatment efficiency is improved, and the application prospect is good.
Owner:CHINA PETROLEUM & CHEMICAL CORP +1

A method for extracting manganese and silver from oxidized manganese silver ore and acidophilic thiobacillus

ActiveCN116855738BThiobacillusPhysical chemistry
The application provides a method for extracting manganese and silver from oxidized manganese silver ore and acidophilic Thiobacillus, and the method comprises the following steps: step 1, crushing and finely grinding the oxidized manganese silver ore and reducing minerals, and then making a slurry to obtain a mineral slurry; step 2, adding sulfuric acid into the mineral slurry and performing chemical leaching, and then performing solid-liquid separation on the obtained chemical leaching product to obtain a chemical leaching residue and a manganese-rich low-acid chemical leaching liquid; step 3, inoculating the chemical leaching residue with the acidophilic Thiobacillus and then performing biological leaching, and then performing solid-liquid separation on the obtained biological leaching product to obtain a low-manganese high-acid biological leaching liquid and a silver-containing biological leaching residue; and step 4, leaching silver from the silver-containing biological leaching residue. The method provided by the application is a full-wet extraction process, and is particularly suitable for treating low-grade oxidized manganese silver ore, can help to reduce the mining boundary grade of manganese silver resources, can improve the resource utilization efficiency, and can obtain greater economic benefits, social benefits and environmental benefits.
Owner:GRINM RESOURCES & ENVIRONMENT TECH CO LTD

In-situ remediation process method for rural black and odorous water body

The invention belongs to the technical field of water environment treatment, and relates to an in-situ remediation process method for rural black and odorous water, which is suitable for ecological treatment of black and odorous water bottom mud and low-temperature regions. The technology comprises the following steps: screening thiobacillus denitrificans, denitrifying bacteria and phosphorus-accumulating bacteria from the bottom mud of a target water area, preparing a special fungicide combination for three stages of deodorization, denitrification and phosphorus accumulation, and carrying out cross antagonism culture; drying-free agglomeration and wrapping granulation are adopted to prepare double-layer particles; injecting into a bottom mud anaerobic layer through a bottom mud injection device in three stages, and adding corresponding microbial agents according to the stages. Through cold-resistant flora directional reinforcement, double-layer particle controlled release and staged precise addition, the microbial inoculum activity and bottom mud permeability in a low-temperature environment are remarkably improved, the problems of microbial inoculum loss, agglomeration and insufficient pertinence are effectively solved, and efficient ecological management of black and odorous water is realized.
Owner:皖创环保股份有限公司

Nitrogen and phosphorus removal method and system based on fully autotrophic nitrification-denitrifying bacteria-algae symbiotic system

PendingCN121872561AWater treatment parameter controlBacteriaSulfurimonasThiobacillus
The invention provides a nitrogen and phosphorus removal method and system based on a fully autotrophic nitrification-denitrifying bacteria-algae symbiotic system, and relates to the technical field of biological nitrogen and phosphorus removal of sewage. According to the method, an autotrophic symbiotic system is constructed by using sulfur autotrophic denitrifying bacteria, autotrophic nitrifying bacteria and common chlorella, main bacteria of the sulfur autotrophic denitrifying bacteria comprise three bacteria of Thiobacillus, Sulfurimonas and Ferritrophum, aeration equipment and an external carbon source are not needed, and efficient nitrogen and phosphorus removal is specially realized for sewage with a low carbon nitrogen ratio (C / N = 1-5). The method comprises the following steps: pre-treating sludge by a sieve, inoculating the sieved sludge into an SBR reactor, and domesticating target bacteria. The chlorella vulgaris is subjected to standardized culture to a logarithmic phase, and is mixed with the domesticated sludge in proportion to form a symbiotic system. According to the system, dissolved oxygen demand conflicts of two bacteria are broken through a light and shade alternation process, the system is kept stable in combination with regular supplement of chlorella, and resource utilization of low-carbon-nitrogen-ratio sewage is realized while energy consumption and treatment cost are reduced by virtue of physiological synergy of bacteria and algae, so that a beneficial solution is provided for environmental protection and sustainable development.
Owner:SHANDONG JIANZHU UNIV

Method for efficiently leaching cobalt in battery positive electrode material tailings treated by wet method

PendingCN121344377AWaste accumulators reclaimingProcess efficiency improvementThiobacillus ferrooxidansThiobacillus
The invention relates to the technical field of cobalt recovery, in particular to a method for efficiently leaching cobalt in battery positive electrode material tailings treated by a wet process, which comprises the following steps: S1, separately culturing thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans and temperature-resistant thiobacillus oxydans; s2, performing compound culture on the separately cultured thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans and temperature-resistant thiobacillus oxydans to obtain a mixed strain solution; s3, the mixed strain liquid is put into an improved culture medium to serve as a leaching system, the cobalt residues obtained through wet treatment are added into the leaching system to be stirred and leached, and a cobalt-rich solution and leaching residues are obtained; the method provided by the invention realizes green, low-carbon and economic recovery of residual metal elements in the wet-process treated tailings, and is short in process, low in investment, high in efficiency, low in treatment cost and suitable for various waste residues containing heavy metals.
Owner:NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA

Feed capable of replacing corn and preparation method of feed

ActiveCN120918263AFood processingAnimal feeding stuffBiotechnologyThiobacillus ferrooxidans
The invention relates to the technical field of feed preparation, in particular to a feed for replacing corn and a preparation method thereof.The preparation method comprises the following preparation steps that S1, pretreated pennisetum sinese is put into a mixed solution of water, ethyl alcohol and acetic acid, and primary filtrate and primary filter residues are obtained; s2, stirring and mixing the primary filtrate obtained in the step S1 and the pretreated edible fungus residues to obtain secondary filtrate and secondary filter residues; s3, mixing the secondary filter residues with water to obtain a mixture, adding thiobacillus ferrooxidans and thiobacillus thiooxidans into the mixture for fermentation, and performing centrifugal filtration to obtain tertiary filter residues; and S4, mixing the third filter residue with the first filter residue and the second filter residue, and adding laccase, lactic acid bacteria, lactobacillus plantarum and saccharomycetes for fermentation to obtain a feed finished product. The feed is prepared through combined fermentation of the edible fungus residues and the pennisetum sinese, corn feed can be effectively replaced, and meanwhile the content of heavy metal in the feed is low.
Owner:JIANGMEN TIANDE BIOTECHNOLOGY CO LTD

Acidophilic thiobacillus and gene scarless knockout method thereof

ActiveCN118726437BShorten knockout timeBacteriaMicroorganism based processesMicroorganismThiobacillus
The application belongs to the field of microorganisms, and discloses an acidophilic thiobacillus and a gene scarless knockout method thereof, which comprises the following steps: recombining an I-SceI gene, an IPTG inducible promoter lacI-PtacO gene and a suicide plasmid pSDUDI to construct a pSDUDI-LacI-I-SceI gene knockout plasmid, and knocking out the I-SceI gene by IPTG induction and expression of I-SceI enzyme; the method improves the probability of the gene knockout plasmid part “separating” from the acidophilic thiobacillus chromosome, simplifies the operation genetic operation step by one-step method, is less time-consuming, and shortens the gene knockout time.
Owner:SHANDONG UNIV