36 results about "Thiobacillus" patented technology

The invention discloses a doping purification process of high-purity quartz sand, and relates to the technical field of quartz sand purification, and the doping purification process comprises the following steps: crushing quartz sand, putting the crushed quartz sand into a microwave reactor, and introducing ozone; immersing the quartz sand subjected to microwave treatment into bacterial liquid, stirring or oscillating, and then separating; the bacterial liquid comprises acidophilic thiobacillus thiooxidans and silicate decomposition bacteria in a mass ratio of 1: (3-10), the concentration of the bacterial liquid is 106-108 CFU / mL, and the pH value is 1.5-3.0; the separated quartz sand is sequentially subjected to high-voltage pulsed magnetic field action and nano bubble flotation; the quartz sand subjected to nano bubble flotation is subjected to acid pickling with a composite acid solution; carrying out pyrolysis film removal on the quartz sand subjected to acid pickling; removing membrane impurities from the quartz sand subjected to pyrolysis membrane removal through a subcritical water repairing process, and filtering, washing and drying; according to the method, micron-sized impurities in the quartz sand can be efficiently separated, impurities of different sizes in the quartz sand are removed step by step, the impurity removal rate is high, the removal effect is good, and the process cost is reduced.

The invention belongs to the field of genetic engineering and resource recycling, and relates to a ferrous oxide recombinant bacterium, a recombination method and application of the ferrous oxide recombinant bacterium in lithium battery metal recovery. The ferrous oxide recombinant bacterium is obtained by taking thiobacillus ferrooxidans as an original strain through genetic engineering modification; comprising thiobacillus ferrooxidans, an exogenously introduced nickel / cobalt efflux protein RcnA gene, a vector pJRD215 and donor bacteria, and the nickel / cobalt efflux protein RcnA gene is introduced into the vector pJRD215, driven to express and then introduced into the donor bacteria to express. The application of the constructed and prepared ferrous oxide recombinant bacteria in lithium battery metal recovery is used for improving the leaching efficiency of metal ions Co < + > or / and Li < + > or / and Ni < + > in lithium battery powder; and the method shows good adaptability and stability under different substrate concentrations, and provides a key experimental support and an important experimental basis for application of a microbial enhanced leaching technology in the field of waste lithium battery resource utilization.

The invention relates to a method for in-situ remediation or management and control of a petroleum contaminated site by micro-nano aeration enhanced microorganisms, which comprises the following steps: 1) injecting micro-nano bubbles into soil or underground water, and continuously aerating for 3-5 days for 6-10 hours every day; an air source of the micro-nano bubbles is one or a combination of two of air and oxygen; 2) 12-24 hours after the step 1) is completed, a compound microbial agent is injected into the soil or the underground water, the compound microbial agent comprises compound microorganisms, and the compound microorganisms comprise pseudomonas, leucas, phenyl bacilli and brevibacillus in the viable count ratio of (1-25): (1-15): (1-5): 1. According to the method, the energy consumption is low, secondary pollution is avoided, the remediation or control effect is excellent, and the total petroleum hydrocarbon (C10-C40) pollutant concentration of the contaminated site after remediation is remarkably reduced.

The invention discloses a water quality conditioning agent based on plant extracts and a preparation method of the water quality conditioning agent, and belongs to the technical field of aquaculture. The water quality conditioning agent is prepared from the following raw materials in parts by weight: 14 to 18 parts of fructus schisandrae chinensis extract, 4 to 5 parts of fructus mume extract, 10 to 13 parts of rhizoma coptidis extract, 8 to 10 parts of glucosamine, 5 to 8 parts of chitosan, 3 to 6 parts of nitrifying bacteria, 2 to 4 parts of thiobacillus thiooxidans and 3 to 6 parts of zeolite powder. Through a four-dimensional synergistic mechanism of'plant active ingredient-chitosan slow release-microbial immobilization-zeolite adsorption ', the pH value, ammonia nitrogen, nitrite, hydrogen sulfide and bottom mud COD in tail water can be reduced and the dissolved oxygen amount can be increased under the condition of one-time addition, which indicates that the water quality regulator has a good effect of improving water quality.

The invention relates to the technical field of solid waste biological treatment, and discloses La and Ce toxicity resistant thiobacillus thiooxidans and application thereof, and the collection number of the thiobacillus thiooxidans is GDMCC No: 63522. The invention provides a novel thiobacillus thiooxidans, and the preservation number of the novel thiobacillus thiooxidans is GDMCC No: 63522. The strain can highly tolerate La and Ce, and can realize biological leaching of solid wastes rich in La or (and) Ce under high ore pulp density, so that more solid wastes can be treated in a single batch, the biological leaching rate is improved, and the strain has a good application prospect.

The invention relates to a metal leaching agent and a method for leaching and recovering metal from a retired photovoltaic module step by step. The metal leaching agent comprises an acidophilic thiobacillus ferrooxidans solution, a hidden acidophilic bacteria solution and a thiobacillus thiooxidans solution, the volume ratio of the acidophilic thiobacillus ferrooxidans liquid to the hidden acidophilic bacteria liquid to the thiobacillus thiooxidans liquid is (0.5-1.5): (0.5-1.5): (0.5-1.5); wherein the concentration of the acidophilic thiobacillus ferrooxidans, the acidophilic hidden bacteria and the thiobacillus thiooxidans is (1-9) * 10 < 8 > cells / mL. Furthermore, the invention provides a method for leaching and recovering metal from the retired photovoltaic module step by step. According to the method, the metal leaching agent is utilized, mild and green recovery of aluminum and silver in the decommissioned photovoltaic cell piece and full-process recovery of the decommissioned photovoltaic module can be achieved through step-by-step biological leaching, and the method has the excellent leaching effect on aluminum and silver.

The invention relates to the technical field of microbial metallurgy, in particular to a method for strengthening leaching of rare earth elements by rare earth-resistant biological acid flora through gradient stress domestication, which comprises the following steps: selecting an original strain of thiobacillus thiooxidans for ultraviolet radiation mutagenesis, and primarily screening mutant strains through a high-throughput screening condition; carrying out compound induction on the culture medium and carrying out four-stage stress domestication on the screened strains; selecting a composite carrier, and curing the domesticated bacterial liquid and the carrier; the industrial microbial inoculum is prepared based on microbial inoculum production and rare earth leaching processes. According to the method for strengthening leaching of the rare earth elements from the rare earth-resistant biological acid flora through gradient stress domestication, rare earth concentration gradient-pH gradient-temperature fluctuation-tailing matrix substitution is constructed as a gradient stress domestication stage, and a composite induction culture medium is combined to realize directed evolution of flora genomes, so that comprehensive improvement of stress resistance indexes is ensured; by designing a composite carrier structure and introducing an immobilization process, a carrier immobilization technology is realized to guarantee industrial stability.

The invention relates to a microbial agent for removing refractory COD (Chemical Oxygen Demand) in wastewater and application of the microbial agent, and belongs to the technical field of biological purification. The invention provides a microbial agent for removing refractory COD (Chemical Oxygen Demand) in wastewater. The microbial agent is prepared from two or more of geomonas, rope warming bacteria, nitro-spirillum, thiobacillus, urea bacillus, leptospirillum, piriformospora, floating fungi, nitro-spina bacteria, thauera and hyphomicrobe. The microbial agent can remove part of refractory COD (Chemical Oxygen Demand) by utilizing the synergistic effect and co-metabolism of biocenosis, and has a great application prospect in the treatment of wastewater (such as landfill leachate and the like) containing a large amount of refractory COD.

The invention relates to a preparation method and application of a microbial fertilizer for preventing and treating citrus diseases, and belongs to the technical field of fertilizer.The microbial fertilizer comprises beneficial microorganisms such as thiobacillus thiooxidans, nitrogen-fixing bacteria and saccharomycetes, humic acid, an AMS-SiO2 carrier, biochemical potassium fulvate and mineral elements. The preparation method comprises the following three key steps: firstly, combining beneficial bacteria with humic acid through a microbial flocculation technology to form a flocculation carrier; secondly, immobilizing paecilomyces lilacinus, bacillus and trichoderma by using the porous AMS-SiO2 carrier to form a complex microbial inoculant; the application of the fertilizer aims at citrus diseases such as citrus huanglongbing, canker and anthracnose, an annular furrow application, broadcast application prevention and chemical pesticide synergistic scheme is adopted, growth period management is combined, and by optimizing the carrier structure and flora compatibility, the fertilizer can be applied to citrus diseases such as citrus huanglongbing, canker and anthracnose. The method solves the problems of low survival rate of microorganisms in a soil environment, disease prevention time mismatch and the like, and obviously improves the disease prevention and control effect and the fruit quality.

The present invention discloses a method for improving the acid tolerance of E. coli by integrating the molecular chaperone CbpA of extreme acidophilic bacteria, belonging to the fields of biochemistry and molecular biology. The present invention provides a molecular chaperone protein CbpA from Acidithiobacillus thermophilum acidophilus and its encoding gene; the molecular chaperone protein CbpA can improve the acid stress resistance of Escherichia coli, and through gene editing technology, the genome of E. coli BL21 is modified to obtain recombinant Escherichia coli. By detecting the growth status and organic acid tolerance of wild-type BL21, defective BL21-ΔcbpA, and recombinant strain BL21-ΔcbpA / AccbpA strains under acidic conditions, its acid tolerance was confirmed. The present invention lays a foundation for later improving the acid tolerance performance of engineering strains through macromolecular repair means such as molecular chaperone proteins, so as to improve the robustness of industrial microorganisms.

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.

The invention discloses a base fertilizer containing a microbial agent for brassicaceous plant broccoli, and belongs to the technical field of microbial fertilizers. The base fertilizer is prepared by the following steps: by taking pig manure and straws as organic raw materials, pretreating the organic raw materials, and mixing the pretreated organic raw materials with a mixture containing neurospora crassa, pseudonocardia alnus, bacillus, streptomyces, paenibacillus mucilaginosus, phanerochaete chrysosporium, azospirillum, trichoderma harzianum, pseudomonas fluorescens and thiobacillus thiooxidans; through staged fermentation, humic acid powder and a trace element mixture are added during aging, and finally bentonite is added for granulation and drying. The base fertilizer can improve the soil structure, adjust microbial communities, inhibit pathogenic bacteria, improve the nutrient utilization rate through nitrogen fixation, phosphate solubilization and potassium solubilization, provide comprehensive nutrition in combination with humic acid and trace elements, promote broccoli root development, enhance stress resistance and realize double improvement of yield and quality; the problems of soil degradation and environmental pollution caused by long-term use of chemical fertilizers are solved, and the requirements of green agriculture development are met.

The invention relates to a cross-feeding complex microbial inoculant capable of synchronously removing beneficiation reagents and heavy metal pollution and application thereof, and belongs to an environmental microbial remediation and wastewater treatment technology, and the cross-feeding complex microbial inoculant is obtained by inoculating a mixed bacterial solution of a sulfur oxidizing bacteria (Thiobacillus.sp) bacterial suspension and a sulfate reducing bacteria (Desulfomacululprofrudii) bacterial suspension into a liquid culture medium, and carrying out shaking culture at 120 rpm under the conditions of constant temperature of 30 DEG C and micro-aerobic condition until the OD600 value reaches 0.6-1.0. According to the cross feeding complex microbial inoculant, through the metabolic synergistic effect of sulfur oxidizing bacteria (Thiobacillus.sp) and sulfate reducing bacteria (Desulfotomacullum profrudii), synchronous efficient degradation of xanthate and synchronous fixation treatment of heavy metals (such as Cu, Pb and Zn) are achieved in a reactor.

The application of the cross-feeding composite microbial agent for simultaneously removing mineral processing reagents and heavy metal pollution belongs to environmental microbial remediation and wastewater treatment technology, and is a mixture of a sulfur-oxidizing bacteria (Thiobacillus.sp ) bacterial suspension and a sulfate-reducing bacteria Desulfotomaculum_profundi ) bacterial suspension. The cross-feeding composite microbial agent is obtained by inoculating the mixed bacterial liquid into a liquid culture medium and culturing at 30 DEG C under constant temperature and micro-oxygen conditions at 120 rpm until the OD600 value reaches 0.6-1.0. The cross-feeding composite microbial agent realizes simultaneous xanthate high-efficiency degradation and heavy metal (such as Cu²⁺, Pb²⁺ and Zn²⁺) simultaneous fixation treatment in one reactor through the metabolic synergistic effect of the sulfur-oxidizing bacteria Thiobacillus.sp ) and the sulfate-reducing bacteria Desulfotomaculum profundi ).

This invention relates to the field of environmental microbiology, specifically disclosing an autotrophic denitrifying bacterial agent and its preparation method. This autotrophic denitrifying bacterial agent employs a three-layer biomimetic design: a sulfur-calcium composite core, an inner shell gel layer, and an outer functional layer. Denitrifying Thiobacillus is loaded into the core, and Pseudomonas schlegelii is fixed in the outer layer, with a sodium alginate-xanthan gum gel layer providing physical isolation, achieving spatial partitioning and symbiosis of autotrophic and heterotrophic denitrifying bacterial communities. The sulfur-calcium composite core uses sulfur powder as an electron donor, and hydrophobically modified eggshell powder supplements alkalinity in situ. The inner shell layer incorporates ultrafine calcium carbonate, providing both mechanical reinforcement and pH buffering functions. The outer layer encapsulates sodium acetate-stearate microspheres for slow carbon source release. This autotrophic denitrifying bacterial agent exhibits high denitrification efficiency, pH stability, and strong resistance to hydraulic shearing under low carbon-to-nitrogen ratio conditions. The preparation process is mild, and the raw materials are inexpensive and readily available, making it suitable for deep denitrification in wastewater treatment and other scenarios.

The invention discloses a method for efficiently leaching nickel in waste power battery positive electrode material wet treatment tailings, which comprises the following steps: collecting the tailings generated by waste power battery positive electrode material wet treatment, and pretreating the tailings to obtain nickel slag; and the mixed bacterial liquid is put into an improved culture medium to form a leaching system, the pH value of the leaching system is adjusted to 1.8-2.2, the temperature of the leaching system is adjusted to 40-45 DEG C, then the nickel slag is added for leaching treatment, and a nickel-rich solution and leaching slag with the nickel content smaller than or equal to 0.1% are obtained through leaching. According to the method, the nickel slag is efficiently leached by using the mixed bacterial liquid compounded by thiobacillus ferrooxidans, leptospirillum ferriphilum, thiobacillus thiooxidans, thiobacillus caldus and temperature-resistant thiobacillus oxydans, nickel can be fully recycled, and the problems of waste of nickel resources and potential environmental pollution of nickel slag treatment are avoided.

The present invention discloses a novel method for treating nitrate in carbon-deficient wastewater using an integrated microbial photoelectrochemical reactor. The method couples TiO2, a nano-semiconductor material with advantages such as stable chemical properties, non-toxicity and high photocatalytic activity, with a Thiobacillus denitrificans biofilm system to form an inorganic semiconductor-microorganism system. Under light conditions, the Thiobacillus denitrificans biofilm uses photogenerated electrons to convert NO3 ‑ This method overcomes the shortcomings of traditional inorganic semiconductor-microorganism integration, such as complex operational processes, reactive oxygen species generated by light that damage bacteria, reduce quantum efficiency, and inhibit nitrous oxide reductase (NOS), resulting in the production of large amounts of greenhouse gases (N2O). It offers the advantages of simple process, low energy consumption, environmental friendliness, economy, safety, and reliability. Furthermore, the system structure is relatively simple, making it easier to modify and improve in a modular manner, thus enabling large-scale promotion and application.

The invention provides a brand-new efficient recovery method for mixed copper-cobalt ore, which comprises the following steps: firstly, crushing and desliming, then inoculating thiobacillus ferrooxidans in a culture medium in which nano iron oxide is pre-dispersed, culturing, and finally adopting an electrochemical flotation system with an anode as a titanium-based platinum-plated electrode and a cathode as a foamed nickel electrode, and flotation is performed in three stages under the control of a pulse power supply. According to the method, a microorganism-assisted electrochemical flotation technology and a nanometer material modification and gradient flotation strategy are combined, the surface property of the ore is changed through microorganism metabolism, metabolism is accelerated through electrochemistry to generate bubbles, flotation selectivity is enhanced with the help of a nanometer material, and finally efficient recovery of the mixed copper-cobalt ore is achieved.

The application provides a wastewater desalination treatment system and treatment process. The wastewater desalination treatment system comprises: a nanofiltration interception module, the nanofiltration interception module comprising a first nanofiltration device, a second nanofiltration device and a third nanofiltration device; a microbial treatment module, the microbial treatment module comprising a sulfate-reducing bacteria reaction device and a Thiobacillus denitrificans reaction device; and an electrodialysis-reverse osmosis module, the electrodialysis-reverse osmosis module comprising an electrodialysis device and a first reverse osmosis device. The wastewater desalination treatment system and treatment process can separate monovalent salt wastewater and divalent salt wastewater from high-salt wastewater through the nanofiltration interception module, the monovalent salt wastewater can be treated by the electrodialysis-reverse osmosis module to obtain recyclable industrial salt sodium chloride, and the divalent salt wastewater can be treated by the microbial treatment module to obtain recyclable elemental sulfur. The sulfate and chloride ions in the high-salt wastewater can be respectively converted into elemental sulfur and sodium chloride for recycling, and the wastewater treatment efficiency is high and the energy consumption is low.

The invention discloses a culture medium for rapidly culturing thiobacillus denitrificans as well as a preparation method and application of the culture medium, and belongs to the technical field of microbial culture. Aiming at the chemical energy autotrophic metabolism characteristics of thiobacillus denitrificans, a highly adaptive, stable and efficient growth environment is cooperatively constructed through precise formula regulation and optimized step-by-step preparation and classified sterilization processes. The culture medium disclosed by the invention can remarkably shorten the growth delay phase of thalli, promote the thalli to quickly enter the logarithmic phase and realize high-density and high-activity stable culture, and the cultured thiobacillus denitrificans show excellent denitrification performance in sewage treatment. The culture medium provided by the invention systematically solves the core problems of long culture period, low efficiency, high cost, poor large-scale stability and the like in the prior art, and has a good application prospect.

The invention provides acidophilic thiobacillus thiooxidans and application thereof, and belongs to the technical field of acidophilic thiobacillus thiooxidans. The acidophilic thiobacillus thiooxidans is named as HS-PLS23-At.t, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34267. The acidophilic thiobacillus thiooxidans is named as HS-PLS23-At.t. Research finds that the acidophilic thiobacillus thiooxidans can grow well when the initial pH is 1-6, and has strong acid resistance. Meanwhile, the acidophilic thiobacillus thiooxidans can generate sulfuric acid from elemental sulfur, so that the acidophilic thiobacillus thiooxidans can efficiently produce sulfuric acid and can also promote leaching of uranium and iron in uranium ores.

The present application relates to the technical field of environmental pollution treatment, in particular to a method for advanced denitrification of secondary effluent of sewage plant, comprising the following steps: S1. taking volcanic rock and sulfur particles as the filler for biofilm adhesion, starting the biofilter column, and after successful start, the functional microorganisms in the biofilm include Thiobacillus and Candidatus Kuenenia; S2. mixing the primary sedimentation tank effluent and the secondary effluent according to the volume ratio of (4.5-y) / (x-4.5)~(5.5-y) / (x-5.5) to obtain the mixed liquor, wherein x and y are the ammonia nitrogen concentrations in the primary sedimentation tank effluent and the secondary effluent respectively, and the unit is mg / L; S3. taking the mixed liquor as the water to be treated for denitrification treatment, and monitoring the water quality of the treated effluent; S4. judging whether to adjust the treatment process according to the nitrogen content in the effluent. The present method can realize the advanced denitrification of the secondary effluent of sewage plant, and has low treatment cost.

The invention discloses an auxiliary agent for biological treatment of sludge containing heavy metals. The auxiliary agent comprises 3 to 8 g / kg of ammonium sulfate, 4 to 6 g / kg of dipotassium hydrogen sulfate, 0.8 to 1.6 g / kg of calcium nitrate, 0.4 to 0.6 g / kg of magnesium sulfate, 2 to 3 g / kg of nicotinamide, 0.4 to 0.6 g / kg of manganese sulfate, 0.9 to 1.6 g / kg of zinc sulfate, 0.6 to 0.8 g / kg of boric acid, 100 to 150 g / kg of ferrous sulfate and the balance of surface modified pyrite powder. The invention also discloses a biological treatment method for sludge containing heavy metals, which comprises the following steps: adding a thiobacillus strain and the auxiliary medicament into the sludge containing heavy metals, stirring until the pH value of a sludge system is reduced to 2.0 or below, transferring the heavy metals from a solid phase to a liquid phase in the process, and then carrying out solid-liquid separation.

The application provides a culture system and culture method of Thiobacillus ferroxidans, wherein the system comprises a nutrient solution tank, a culture tank, a communication device, an overflow separator, a clear liquid tank, a sedimentation tank and a reflux device, the overflow separator is provided with a solid discharge control valve at the bottom, the precipitate is discharged from the solid discharge control valve, and the overflow liquid returns to the culture tank, so that the precipitate and the Thiobacillus ferroxidans are separated; the culture method realizes the efficient culture of the Thiobacillus ferroxidans through the steps of preparing a nutrient solution, inoculation, aeration culture, circulating flow addition, precipitation separation and continuous culture, solves the problem that the precipitate such as iron alum affects the growth of microorganisms in the culture process, guarantees the fresh culture liquid supply, promotes the continuous propagation and growth of the microorganisms, and thus improves the output efficiency and the living quality of the Thiobacillus ferroxidans. The obtained microbial suspension can be prepared into a microbial agent product and can also be directly used in the fields of bio-metallurgy, environmental remediation, bio-electrochemistry and bio-fuel cells.

The application relates to the technical field of sewage treatment, and more specifically to a sulfur autotrophic denitrification process, which is prepared by combining sulfur-calcium carbonate eutectic granulation with PTFE microporous coating technology, and has the functions of pH self-buffering and anti-blocking; three functional zones are arranged in the reactor: the upper layer is filled with coated sulfur particles and inoculated with Thiobacillus bacteria, calcium carbonate is used to neutralize H + The coated layer controls the excessive proliferation of the biofilm, the nitrogen gas is promoted to escape under high flow rate, the middle layer of the honeycomb ceramic is inoculated with anaerobic string bacteria to degrade residual nitrite nitrogen, the flow rate is reduced to 5-8 m / h to prolong the residence time, the lower layer of the magnesium olivine cooperates with the inclined plate precipitation module to realize efficient separation of sulfate precipitation and shed biofilm under low flow rate. Through water inlet pH pre-adjustment, DO < 0.5 mg / L and gradual load domestication, the functional bacteria group can tolerate pH 5.5-8.0, and the problems of large denitrification efficiency fluctuation, nitrite nitrogen accumulation and filler blockage in the traditional process are solved, and the system realizes efficient, impact-resistant and long-term stable operation.

The present invention relates to a method for producing a bacterial microcompartment virus-like particle (VLP) carrying a cargo molecule, the method comprising introducing and expressing in a host cell or organism one or more polynucleotides comprising (a) a first sequence encoding bacterial microcompartment shell protomers and a second sequence encoding a cargo molecule fused to an encapsulation peptide comprising the sequence SKITGSSGNDTQGSLITYSGGARG, and forming a microcompartment that encapsulates the cargo molecule, or (b) a first sequence encoding bacterial microcompartment shell protomers and a second sequence encoding at least one of said protomers fused with a cargo molecule or a biochemical tag, and forming a microcompartment that expresses the cargo molecule or biochemical tag on an exterior surface. In one embodiment, the bacterial microcompartment protomers are CsoSIA and CsoS4A from Halothiobacillus neapolitanus, or HO-H, HO-P and HO-T1 from Haliangium ochraceum.

This invention belongs to the field of biological wastewater treatment, specifically relating to a composite microbial agent, its preparation method, and its application. By mass percentage, it comprises 60%-80% bioactive agent and 20%-40% activity protectant. The bioactive agent includes 66.58%-86.58% of *Haloxylon ammodendron* strains, 7.85%-22.85% of *Hymenobacter* strains, and 5.57%-25.57% of *Thiobacillus* strains. The total viable count of the composite microbial agent is not less than 1 × 10⁻⁶. 9 The compound microbial agent was added to distilled water and cultured under anaerobic shaking conditions to obtain a revived bacterial solution. The supernatant of the revived bacterial solution was mixed with the wastewater to be treated at a volume ratio of 1-10:250, and 100-250 mg / L of a compatible solute was added. The wastewater was then treated under anaerobic conditions. After one week of treatment of low C / N ratio, high-salt phenol wastewater using this compound microbial agent, the denitrification rate remained above 95%, and the phenol degradation rate remained above 80%. This effectively solved the problems of low denitrification efficiency and difficulty in removing phenolic compounds from high-salt phenol wastewater, and the process was simple and low-cost.

The invention relates to the technical field of biological treatment of solid wastes, and discloses thiobacillus thiooxidans resistant to toxicity of Ni and Cu and application of the thiobacillus thiooxidans. Wherein the thiobacillus thiooxidans is the thiobacillus thiooxidans QH2C, and the preservation number of the thiobacillus thiooxidans is GDMCC No: 63521. The invention further discloses a preparation method of the thiobacillus thiooxidans. The invention provides a novel thiobacillus thiooxidans QH2C, and the strain has high toxicity tolerance to Ni and Cu, so that the strain can be used for treating metal-containing solid wastes with higher ore pulp density in batches, the biological leaching treatment efficiency is improved, and the application prospect is good.

The application provides a method for extracting manganese and silver from oxidized manganese silver ore and acidophilic Thiobacillus, and the method comprises the following steps: step 1, crushing and finely grinding the oxidized manganese silver ore and reducing minerals, and then making a slurry to obtain a mineral slurry; step 2, adding sulfuric acid into the mineral slurry and performing chemical leaching, and then performing solid-liquid separation on the obtained chemical leaching product to obtain a chemical leaching residue and a manganese-rich low-acid chemical leaching liquid; step 3, inoculating the chemical leaching residue with the acidophilic Thiobacillus and then performing biological leaching, and then performing solid-liquid separation on the obtained biological leaching product to obtain a low-manganese high-acid biological leaching liquid and a silver-containing biological leaching residue; and step 4, leaching silver from the silver-containing biological leaching residue. The method provided by the application is a full-wet extraction process, and is particularly suitable for treating low-grade oxidized manganese silver ore, can help to reduce the mining boundary grade of manganese silver resources, can improve the resource utilization efficiency, and can obtain greater economic benefits, social benefits and environmental benefits.

The invention belongs to the technical field of water environment treatment, and relates to an in-situ remediation process method for rural black and odorous water, which is suitable for ecological treatment of black and odorous water bottom mud and low-temperature regions. The technology comprises the following steps: screening thiobacillus denitrificans, denitrifying bacteria and phosphorus-accumulating bacteria from the bottom mud of a target water area, preparing a special fungicide combination for three stages of deodorization, denitrification and phosphorus accumulation, and carrying out cross antagonism culture; drying-free agglomeration and wrapping granulation are adopted to prepare double-layer particles; injecting into a bottom mud anaerobic layer through a bottom mud injection device in three stages, and adding corresponding microbial agents according to the stages. Through cold-resistant flora directional reinforcement, double-layer particle controlled release and staged precise addition, the microbial inoculum activity and bottom mud permeability in a low-temperature environment are remarkably improved, the problems of microbial inoculum loss, agglomeration and insufficient pertinence are effectively solved, and efficient ecological management of black and odorous water is realized.