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179results about How to "Short breeding time" patented technology

Cuttage rapid-propagation seedling culture method for elevated sand bed camphora wood cutting orchard tissue culture seedling twigs

The invention discloses a cuttage rapid-propagation seedling culture method for elevated sand bed camphora wood cutting orchard tissue culture seedling twigs. The method comprises the following steps: taking semi-lignified twigs formed by camphora wood cutting orchard tissue culture seedling planted on the elevated sand bed as a cutting wood; treating the cutting wood by greenplant growth regulator-ABT6 rooting solution; inserting the treated cutting wood into a mixed medium which contains peat soil and coconut bran in a weight ratio of 3:(1-1.5); culturing on a pressure lifting seedling culture bed capable of automatically controlling temperature, controlling moisture and controlling light; controlling parameters of air humidity to 85%, medium moisture content to 50% and illumination intensity to 1500lux; and after one month, causing the cutting wood to form an integral plant, wherein the cuttage survival rate is 80-85%. The method has the advantages of scientific seedling culture facility, high propagation speed, large seedling culture amount, short seedling culture time, high afforestation survival rate, low wood culture cost, good economic benefit and social benefit and the like, offspring can keep the excellent hereditary character of a stock plant, and the asexual seedling culture of camphora fine variety is achieved.
Owner:GUANGXI FORESTRY RES INST

Method for rapidly identifying resistance to verticillium wilt of eggplant

InactiveCN103355025ASame degree of root damageEnsure consistencySpore processesHorticultureSporeHigh survival rate
The invention provides a method for rapidly identifying resistance to verticillium wilt of eggplant. The method comprises the following steps of: accelerating germination and sowing of eggplant seeds, seedling culturing, preparation of a verticillium wilt spore suspension, treatment of pathogenic bacteria infection and incidence investigation. According to the invention, eggplant seedlings are infected with verticillium wilt in a manner of soaking and infecting seedling roots by a bacteria solution, and the eggplant seedlings are infected after being uniformly cultured in a substrate until achieving a state of one seedling with two leaves, so that the growth vigor and size of the eggplant seedlings are easy to control, and the consistency and reliability of an identification result are ensured. By adopting the appropriate verticillium wilt substrate and adjusting the concentration of the verticillium wilt suspension, the method has the advantages of good infection effect, high survival rate of the infected seedlings, uniform inoculation and rapid incidence. According to the invention, only different plants with consistent growth vigor need to be soaked in the bacteria solution after root cutting, so that the operation is simple and convenient, the seedling culture time is short, and the method is particularly suitable for identification of resistance to verticillium wilt of eggplant breeding materials in batches.
Owner:SHENYANG AGRI UNIV

Rapid rhubarb seedling growing method

The invention discloses a rapid rhubarb seedling growing method. The rapid rhubarb seedling growing method includes the following steps: 1), building a seedling growing pool or picking a seedling growing basin and adding water; 2), picking and sterilizing a seedling growing plate, placing a seedling growing base material into the seedling growing plate, and sowing pre-treated rhubarb seeds into the seedling growing plate to acquire the seedling growing plate subjected to sowing, wherein the seedling growing base material comprises, by weight, 50-70% of peat and 30-50% of perlite; 3), placing the seedling growing plate subjected to sowing in the step 2) into the seedling growing pool or seedling growing basin in the step 1) for cultivation, and after seedling emergence, adding fertilizer into water in the seedling growing pool or seedling growing basin once every ten days until nitrogen concentration is 150-250g/L, phosphorus concentration is 10-40g/L and potassium concentration is 200-300g/L; 4), completing cultivation after the diameters of rootstocks of rhubarb seedlings are not less than 1cm and roots and the rootstocks are 5-10cm in length. The rapid rhubarb seedling growing method is simple and convenient to operate, high in seeding emergence rate, short in time, low in cost and good in economic benefit, and the rhubarb seedlings grown by the method grow rapidly, orderly and healthily.
Owner:SICHUAN ZHIJIACHENG BIO TECH CO LTD

Denitrifying filler, preparation method of denitrifying filler and application of denitrifying filler to denitrification of water body

The invention provides a denitrifying filler, a preparation method of the denitrifying filler and application of the denitrifying filler to denitrification of a water body. On the first aspect, a surface modifier is coated on the surface of sulfur granules, and the adsorptivity of the surface modifier and the positive charge on the surface modifier facilitates a microbial flora with negative charge to be grown on the surface of the sulfur granules in an attaching way; on the second aspect, heterotrophic denitrifying bacteria are increased in a conventional autotrophic denitrification microbic system to form a mixotrophic denitrification system, so that the breeding time is short, the antijamming capability is strong, the hydraulic retention time is relatively shortened, and the stability of the system and the adaptability of the system to external conditions are also improved; on the third part, the defects that secondary pollution is easily caused by overhigh sulfate ion content in effluent of a sulfur-based autotrophic denitrification system, land salinization is caused, and reusing of the effluent is not benefited are also greatly reduced by the denitrifying filler, and the denitrification performance is improved.
Owner:HUBEI UNIV

Artificial seedling method of blood clam

InactiveCN101161065AShort breeding timeRestoring population dominanceUnicellular algaeClimate change adaptationShrimpAlgae
A method of artificial breeding for ark shells, which includes cultivating unicellular algae first, then breeding, sampling and inspecting parent calms gonads of ark shells in the sea, capturing and transporting parent calms, breeding species shells temporarily in seeding room interiorly; spawning and hatching parent calms; selecting and breeding larvae, cultivating larvae, changing water once a day, scattering bait 30,000-100,000 / ml single cells every day; scattering cultch when the body length of larvae is 230 micrometers, changing water totally every day, moving larvae to the middle of the sea or shrimp tank to breed temporarily when the shells of them are 0.7-1.2mm; beating platform raft firstly as temporary breeding, the direction of which and flow direction being almost vertical; temporary breeding management, tying one hanging at intervals of 0.6-1.0m on every platform raft, 6-10 bags on every hanging, 5,000-15,000 juveniles shellfishes in every bag, arranging platform raft, promising the safety of platform raft, scattering on the bottom of the sea or cultivating in the shrimp tank when juveniles shellfishes grow up above 5mm. The advantage of the method can realize artificial breeding, short time of breeding, cultivation in the shrimp tank, bottom sowing in the sea and resume the population advantage, low-cast, quick results and great benefit.
Owner:JINZHOU MARINE FISHERY INST

Method for producing edible sunflower hybrid seeds

The invention discloses a method for producing edible sunflower hybrid seeds. The method comprises the following steps of: (1) culturing parents a, namely planting an edible sunflower variety a which has high combining ability and good economic characteristic in a production site; and through 5 to 8 generations of sister hybridization, selecting strains which have strong adaptability and high purity without degeneration after the sister hybridization as the parents a; (2) culturing parents b, namely planting an edible sunflower variety b which has the same flowering phase, maturation phase and strain height as those of the parents a, and has disease resistance and seed size complementary for those of the parents a; and after 5 to 8 generations of self-copulation, selecting the strains which have the high combining ability as the parents b; and (3) producing hybrid seeds, namely planting the parents a and the parents b at the same time; and mutually pollinating the parents a and the parents b in the flowering phase in order to culture the hybrid seeds. The method for producing the edible sunflower hybrid seeds has the characteristics of simple processes, low cost, randomly selected hybrid combination and strong combination advantages. The hybrid seeds have the advantages of disease resistance, high yield, good quality, strong growth potential and the like.
Owner:NORTHWEST A & F UNIV

Cultivation method of high quality cuttings from semi-mangrove Cerbera manghas

A cultivation method of high quality cuttings from semi-mangrove Cerbera manghas is characterized in that fruits on good, healthy mother trees with no pest or disease and growing for more 10 years are selected, seeds are extracted from the fruits by a fruit drying and husking machine, the seeds are soaked in hot water of 85 DEG C, the seeds are soaked at constant temperature after the seeds naturally cool to 40 DEG C, the seeds are sprouted on an electric heating sand bed with temperature of 20-25 DEG C and humidity of 60-80%, plantlets which sprout in a first batch, bear two pairs of complete leaves each and grow 10cm tall are transplanted to a seedling bed used for cultivating seedlings of Cerbera manghas, seedlings are cultivated on the seedling bed as a cutting bed, semi-lignified shoots are cultivated as cuttings, the cuttings are inserted in pots, each made with 2-4 parts of yellow subsoil and 6-8 parts of fine sand, to cultivate seedlings. The method has the advantages that the use of the electric heating sand bed increases sprouting rate of the seeds of Cerbera manghas and shortens cultivation time of mother plants of seedlings; slow release fertilizer as base fertilizer and quick-acting fertilizer is applied to the planted mother plants for topdressing, nutrition required by the mother plants during the initial growth period can be guaranteed, continuous supply of nutrition in the whole growth period is guaranteed, growth is fast, buds are many and strong, and seedlings are good in quality.
Owner:GUANGXI FORESTRY RES INST

Novel beak-shaped litchi planting method capable of achieving high and stable yield

The invention discloses a novel beak-shaped litchi planting method capable of achieving high and stable yield. The novel beak-shaped litchi planting method includes the steps of a), selecting and treating cuttings, namely selecting strong branches from seed trees, cutting the branches to form cuttings, soaking to sterilize roots of the cuttings, and then soaking the roots of the cuttings in a root inducing agent; b), treating seedbed fields, namely deeply ploughing seedbed soil, applying one layer of red clay and peat soil on the seedbed soil, scarifying the seedbed soil again, sterilizing, applying ripe cow dung, superphosphate and a potash fertilizer for 3-5 days after sterilization, watering to drench the seedbed soil and sterilizing; c) performing litchi cutting and management, namely inserting the litchi cuttings into the seedbed soil obliquely, spraying quick lime and ABT rooting powder after cutting, watering seedbeds every day, sterilizing after 10 days, spraying nutrient solution for every 10 days after sterilization, and cultivating till seedlings emerge. By the aid of a cuttage method, the novel beak-shaped litchi planting method is short in seedling time, high in survival rate, low in cost and capable of keeping excellent characteristics of female parents, accelerating litchi raising, shortening fruit bearing period and increasing litchi yield.
Owner:合浦县公馆镇香山村马拉坡鸡嘴荔枝农民专业合作社

Method for obtaining excellent clone through fiber bamboo subterraneous stem in-situ multiple mutagenesis

ActiveCN110574681ADirected genetic improvementEasy to operatePlant genotype modificationEthylmethane SulfonateFiber
The invention discloses a method for obtaining an excellent clone through fiber bamboo subterraneous stem in-situ multiple mutagenesis. The method comprises the steps that a subterraneous stem of fiber bamboo is selected; an internal irradiation mutagenic agent is selected to be prepared into mutagenic agent solutions separately; a chemical mutagenic agent ethylmethane sulfonate is selected to beprepared into mutagenic agent solutions separately; a stainless steel continuous syringe is used for filling different segments of the selected subterraneous stem of the fiber bamboo with the internalirradiation mutagenic agent of different concentrations, and injection ports are sealed by non-sticking adhesive tapes; after 30-40 days, the stainless steel continuous syringe is used for filling the different segments of the selected subterraneous stem of the fiber bamboo with the chemical mutagenic agent solution, and the injection ports are sealed by non-sticking adhesive tapes; after earthing is performed for 60-90 days, different mutagenic buds on the segments, subjected to multiple treatment of the internal irradiation mutagenesis and mechanical mutagenesis, of the subterraneous stem are germinated into bamboo, and through screening and underground root dividing propagation, the clonal new germplasm or new species of the fiber bamboo with excellent traits.
Owner:SOUTHWEAT UNIV OF SCI & TECH

Cutting propagation method of ricinus communis

InactiveCN102823409AAvoiding the Difficulty of Finding a RetainerEasy to maintainCultivating equipmentsSoilless cultivationPropagation timeGreenhouse
The invention discloses a cutting propagation method of ricinus communis, which belongs to the technical field of crop propagation. The method comprises the following steps of: cutting ricinus communis branches of 1-2 years old, retaining one leaf with the leaf area being 20%-25% of the original leaf area on each branch, dipping the cut branches with rooting powder and then inserting into a seedling bed, keeping the air humidity to be 100% in a cutting seedling greenhouse during the period from cutting to rooting. The method provided by the invention overcomes difficulties of the ricinus communis such as large areas, fast moisture evaporation, hollow stems, high lignification extent and difficult rooting; the inarched ricinus communis branches are capable of successfully rooting within a short time of 40 days more or less; the rooting rate is 53%-86.7%, and speediness, orderliness, strong growth vigour and good growth can realized in rootage; the propagation time is short, the operation is concise and easy to master. The invention provides a novel method for protecting the ricinus communis resource, which is capable of keeping excellent individual plants of the ricinus communis and shortening the propagation time. The application of the method provided by the invention in the propagation of female individual plants of the ricinus communis is particularly significant in heterosis utilization.
Owner:云南省农业科学院经济作物研究所

Method for growing seedlings of baical skullcap root by cutting

The invention discloses a method for growing seedlings of baical skullcap root by cutting, belonging to the technical field of traditional Chinese medicinal material cultivation. The method specifically comprises the following seven steps: selection of mother plants for cutting, cutting preparation, plant hormone treatment, cutting, water sprinkling management, shed putting-up for shading and seedling hardening. By adopting the method, the defects of reduction of the content of an effective component, baicalein, reduction of effects, low survival rate and poor growth appearing when the traditional Chinese medicinal material baical skullcap root is artificially cultivated are effectively overcome. The method has the beneficial effects that the materials are convenient to take; the processes are simple; the rooting rate of baical skullcap root is 79.4-85.3%; the seedling growing time is short and the seedlings can come out from nurseries and be transplanted in less than one month; the content of the effective component baicalein is more than 13.8%, is far beyond the stipulation of not lower than 4% in the Chinese pharmacopoeia and is also higher than that in sexual propagation and wild samples; as the seedlings have 3-4 main roots, and fast growing and stable and high yield are achieved after the seedlings are transplanted.
Owner:黄振忠

Bovine viral diarrhea virus (BVDV) transgenosis Astragalus mongholicus vaccine and production method

The invention relates to a bovine viral diarrhea virus (BVDV) transgenosis Astragalus mongholicus vaccine and a production method which are used for preventing and curing animal bovine viral diarrhea virus, and overcoming the defects of poor immunogenicity and low protection of the existing inactivated vaccines and nucleic acid vaccines as well as unsafe application of the existing live poison vaccine. In the invention, BVDV RNA is taken as a template, a reverse transcription-polymerase chain reaction method is used for obtaining an E0 gene thereof, then a gene E0 recombination plant expression vector is constructed, a pollen tube pathway method is used for obtaining the BVDV E0 gene transgenosis Astragalus mongholicus, and the Astragalus mongholicus with immunogen transformed by the BVDV E0 gene is screened by an immunoblotting and an enzyme linked immunosorbent assay to obtain a vaccine product. The invention has the following beneficial effects: the BVDV E0 gene is expressed in the Astragalus mongholicus, which generates the protective antigen and adjuvant substance capable of increasing the immunization, thereby improving the antiviral effect of the Astragalus mongholicus and the optimun immunity of the vaccine; and the invnetion has the advangtages of easy production, convenient use, strong stability and no side effects.
Owner:JILIN AGRICULTURAL UNIV
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