5results about How to "Short breeding time" patented technology

The invention relates to the technical field of genetic engineering breeding, in particular to application of ZmC2DP1 protein in regulation and control of drought resistance and salt tolerance of plants, the amino acid sequence of the ZmC2DP1 protein is as shown in SEQ ID NO.1, and the nucleotide sequence of a gene for coding the ZmC2DP1 protein is as shown in SEQ ID NO.2. After the ZmC2DP1 gene is knocked out, the drought resistance and the salt resistance of a mutant plant under the drought treatment or salt treatment condition are improved compared with those of a control group plant, and it is verified that the ZmC2DP1 protein has a negative regulation function on the drought resistance and the salt resistance of the plant. Compared with a traditional breeding mode, the method for regulating and controlling the drought resistance and the salt resistance of the plants has the advantages that the breeding time is short, the purposiveness is high, the drought resistance breeding period is remarkably shortened, and the crop stress resistance breeding efficiency is improved; a gene editing homozygous strain with improved drought resistance and salt tolerance is obtained by adopting a gene knockout technology, and gene resources are provided for cultivating and improving new varieties of stress-resistant plants; and a theoretical basis is provided for clarification of a molecular mechanism of the ZmC2DP1 protein in drought resistance and salt resistance of plants.

The invention relates to application of a potassium efficient gene DT1 in improvement of drought resistance of corn. It is found that the DT1 gene can significantly improve the drought resistance of corn, by overexpressing the DT1 gene in corn, a corn plant with higher drought resistance is successfully constructed, after drought treatment, the relative water content of leaves of the constructed DT1 overexpressed plant is higher than that of wild type leaves, the water loss rate of in-vitro leaves is reduced, and the drought resistance is significantly enhanced; and a gene resource is provided for cultivating a new variety. Compared with a traditional breeding mode, the drought-resistant breeding method has the advantages that the breeding time is short, the purposiveness is high, the breeding period is remarkably shortened, the breeding efficiency is improved, the drought-resistant breeding method has great application value in crop molecule improvement, and a theoretical basis is provided for clarification of a molecular mechanism of plant drought resistance.

The breeding method comprises the following steps: firstly, selecting male fast-growing green-shank and white-feather broilers as progenitor male parents, fast-growing female green-foot partridge chickens as progenitor female parents 1 and fast-growing female iron-foot partridge chickens as progenitor female parents 2, and separately breeding the fast-growing green-shank and white-feather broilers, the fast-growing female green-foot partridge chickens and the fast-growing female iron-foot partridge chickens; the female chickens are fed with daily ration added with 800 IU / kg of vitamin D3 and 500 IU / kg of vitamin E after three weeks old; recessive white feather broilers with the weight larger than 2700 g after 150-160 days are selected to be fed to the progenitor male parents, and partridge chickens with green feet and partridge chickens with the weight larger than 1800 g after 170-180 days are selected to be fed to the progenitor female parents 1 and the progenitor female parents 2; hybridizing the progenitor male parent with the progenitor female parent 1, hybridizing the green-shin male offspring as a male parent, hybridizing the progenitor male parent with the progenitor female parent 2, and hybridizing the green-shin female offspring as a female parent; carrying out parent cultivation; and screening the male parent and the female parent, and hybridizing the male parent and the female parent to obtain a commercial generation. By means of the method, the fast-growing green-shin sparkling broilers which are high in growth speed, good in uniformity, high in survival rate and good in economic benefit are obtained.

This invention discloses a method for rapid propagation and seedling cultivation of *Gynostemma pentaphyllum* seedlings. Healthy, disease-free *Gynostemma pentaphyllum* plants are selected. Young leaves are washed to remove surface impurities and then disinfected. The disinfected explants are cut into pieces and inoculated into an adventitious bud induction medium to induce callus tissue. The induced callus tissue is then transferred to an adventitious bud differentiation medium to differentiate adventitious buds. The differentiated adventitious buds are then cut off of their leaves, retaining the stem segments with nodes, and inoculated into a proliferation medium. Proliferating buds with good growth and large, thick leaves are inoculated into a rooting medium. The rooted seedlings are then placed in a greenhouse for hardening off. The hardened rooted seedlings are then cleaned of the root culture medium before further cultivation. The tissue-cultured rooted seedlings produced by this method are robust, red in color, and have well-developed root systems. They exhibit high survival rates after cultivation and have a short cultivation time, providing technical support for the large-scale, standardized production of *Gynostemma pentaphyllum* and possessing broad application prospects.

The invention discloses a ZmSUS2 protein and application of a coding gene thereof in plant drought resistance, the amino acid sequence of the ZmSUS2 protein is shown as SEQ ID No.1, and the nucleotide sequence of the coding gene of the protein is shown as SEQ ID No.2. After the ZmSUS2 gene is knocked out from a plant and drought stress treatment is carried out, the drought resistance of a mutant plant is improved compared with that of a control group. The invention further discloses a method for improving the drought resistance of the corn, the ZmSUS2 gene is knocked out from a receptor plant, and the corn with high drought resistance is obtained. The invention verifies that the ZmSUS2 protein and the coding gene thereof have a negative regulation function on the drought resistance of the corn, and an excellent candidate gene resource is provided for cultivating and improving a new variety of drought-resistant corn; according to the provided drought-resistant plant breeding method, the breeding process is more efficient, reliable, accurate and controllable, and the method has important theoretical and practical significance for improving crop resistance and accelerating the corn breeding process.