744 results about "Drought stress" patented technology

A rapid monitoring and identification method for winter wheat drought levels includes: 1) obtaining multispectral images and ground-measured LAI through UAV-based multi-payload low-altitude remote sensing technology, and calculating vegetation indices such as Normalized Difference Vegetation Index (NDVI), Difference Vegetation Index (DVI), Ratio Vegetation Index (RVI), Enhanced Vegetation Index (EVI), Optimized soil adjusted vegetation index (OSAVI) and Transformed Chlorophyll Absorption Reflectance Index; 2) establishing regression equations between the calculated vegetation indices and measured LAI for different growth stages, and selecting the optimal model equation for each growth stage; 3) using the optimal model equation to invert the LAI of winter wheat at various growth stages, and calibrating the LAI thresholds for different drought stress levels; 4) acquiring the multispectral images of target plot through real-time monitoring, calculating the required vegetation indices, inverting to obtain the LAI value, and comparing it with the threshold to determine the current drought stress level.

The invention belongs to the technical field of intelligent agriculture, and discloses a canopy-atmospheric environment information-based soil water content prediction method and system, and the method comprises the steps: setting a canopy and atmospheric environment monitoring unit in a field, and synchronously collecting the canopy temperature, wind speed, relative humidity, and six-dimensional environment parameters of atmospheric temperature, wind speed and relative humidity; the crown temperature difference is calculated after preprocessing; the method comprises the following steps: constructing a training set by utilizing measured data of soil relative water content, constructing a soil water content prediction model by adopting a random forest algorithm, screening and confirming an optimal prediction model through grid search and cross validation, and screening key features by combining an SHAP value; the water demand is automatically calculated according to a pre-established crop whole-growth-period drought stress threshold table and a dynamic irrigation decision formula, and meanwhile, the adaptation of a general model to a regional specific model is realized by constructing an environmental parameter-actually measured water content database and transfer learning, so that the soil water content state is accurately predicted, and the soil quality is improved. And a scientific basis is provided for precise irrigation.

The invention relates to a Chinese wildrye rhizosphere growth-promoting bacterium and application of the Chinese wildrye rhizosphere growth-promoting bacterium in growth promotion and drought resistance of Chinese wildrye, and belongs to the technical field of microorganisms, the strain is classified and named as Paraburkholderia graminis, the strain number is B24, and the preservation number is CGMCC No.31907. The strain provided by the invention is derived from the intergrassland soil of leymus chinensis in an excessive grazing grassland, has good growth promoting characteristics of producing IAA, producing ACC deaminase, fixing nitrogen, dissolving phosphorus and the like, and also can obviously enhance the drought resistance of the leymus chinensis. When the strain is inoculated to the rhizosphere of a leymus chinensis seedling, the growth of the leymus chinensis can be obviously promoted, and the overground and underground biomass of the leymus chinensis is increased; the activity of antioxidant enzymes is reduced and the hormone content in leymus chinensis leaves is changed, so that drought stress is relieved, the photosynthesis of leymus chinensis is enhanced, and the drought resistance of leymus chinensis is remarkably improved. Therefore, the Burkholderia parakholderia can be used for development of a microbial fertilizer for grassland plants and applied to a drought stress habitat, so that the drought resistance of the grassland plant leymus chinensis is improved, and grassland management is optimized.

The invention relates to the technical field of drought stress, and discloses a method for identifying a vegetation water stress dominant factor and determining a drought threshold value based on a Copula function. The method comprises the following steps: acquiring vegetation productivity, soil water content and atmospheric water deficit data of a research area in a research time period; respectively constructing marginal distribution functions of the vegetation productivity, the soil water content and the atmospheric water deficit by using a univariate parameter estimation method; respectively constructing a joint probability distribution function of the soil water content and the vegetation productivity and a joint probability distribution function of the atmospheric water deficit and the vegetation productivity based on the marginal distribution function by utilizing a Copula function; respectively calculating the maximum conditional probability of vegetation productivity loss caused by soil water content reduction and atmospheric water deficit rise by utilizing the joint probability distribution function, and determining a vegetation productivity water stress dominant factor; and calculating a drought threshold value of the vegetation productivity water stress dominant factor.

The invention discloses a comprehensive evaluation method for drought resistance of white birch germplasm resources in the seedling stage, and belongs to the technical field of drought resistance evaluation. Under different gradient drought stress and normal conditions, 14 drought resistance indexes of 90 white birch half-sib families are measured, and the drought resistance is comprehensively evaluated by combining membership function analysis, principal component analysis and clustering analysis. A comprehensive drought resistance evaluation value (D value) is obtained according to a membership function, comprehensive clustering analysis is carried out according to the D value of each family, 90 families are divided into four categories at the Euclidean distance of 1.5, and BWY32 and BWY29 are screened out as drought-resistant families. A stepwise linear regression analysis method is utilized to respectively establish linear regression equations under drought stress 5d and 10d, and four important drought resistance indexes are screened out. Compared with the previous research, the method fully considers the difference of the sensitivity degree of each selected index to drought stress and the contribution rate of each index to drought resistance evaluation, and lays a good foundation for simplification and high efficiency of the drought resistance evaluation of the white birch.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to improve the corn plant's drought tolerance and ultimate production yield in hot weather. By selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then infused to the soil in which the corn seeds and / or seedlings are planted. Optionally, the KHP solution can be diluted by water, as disclosed in the specification, for applying to the soil.

The invention discloses a vegetation resistance and resilience evaluation system and method under drought stress, and belongs to the field of global change ecology research. Comprising the following steps: collecting related data of vegetation resistance and restoring force by using a data collection module, and inputting the related data of vegetation resistance and restoring force into a data storage module for storage, a vegetation physiological index processing sub-module, a vegetation structure index processing sub-module and a vegetation function index processing sub-module in the data processing module are called to process the collected and stored data; and calling a lag accumulation correlation coefficient calculation sub-module, a vegetation resistance index and restoring force index evaluation model sub-module and a vegetation resistance and restoring force grade division sub-module in the analysis and evaluation module, and finally outputting vegetation resistance and restoring force evaluation grades. According to the method, comprehensive, systematic and accurate evaluation and grading can be carried out on the resistance and the restoring force of the vegetation under drought stress, the vegetation which is difficult to resist drought or is restored after drought stress in the evaluation area is effectively identified, and the method is simple to operate and easy to realize.

The invention discloses application of an RLCK86 gene in improvement of drought tolerance of tomatoes, and belongs to the technical field of biology. According to the application, the tomato RLCK86 gene function is silenced or deleted by utilizing a biological technical means, so that the tolerance of tomato plants to drought stress is improved; the nucleotide sequence of a protein coding region of the RLCK86 gene is as shown in SEQ ID NO. 1. The invention discloses the application of the RLCK86 gene in regulating and controlling the drought stress resistance of the tomato for the first time, the function of the RLCK86 gene is deleted through a gene editing technology, the drought tolerance of the tomato can be remarkably improved, and a reference is provided for cultivating drought-resistant tomato germplasm resources.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The invention provides a method for screening drought-enduring germplasm resources of macadamia nuts, and belongs to the technical field of plant germplasm resource screening. The screening method comprises the following steps: (1) sowing disinfected macadimia nut seeds into a seedling raising tray, and raising seedlings to obtain seedlings; (2) transplanting the seedlings obtained in the step (1) into a nutrient medium, and performing drought stress culture to obtain pretreated seedlings; and (3) determining drought tolerance indexes in the pretreated seedlings obtained in the step (2), and screening to obtain the macadimia nut drought-tolerant germplasm resources. According to the screening method provided by the invention, macadamia nut germplasm resources with relatively strong drought tolerance can be rapidly screened out, and powerful support is provided for cultivation of drought-tolerant varieties.

The invention belongs to the technical field of drought risk prediction and vegetation influence evaluation, and particularly discloses a vegetation-to-drought resource utilization coupling response strategy evaluation method, which comprises the following steps: acquiring target data; constructing a drought index according to the target data, and extracting a drought event; extracting vegetation total productivity, vegetation net level productivity and evapotranspiration abnormal data before and after the drought event, and calculating response intensity of vegetation variables; calculating the average variation of the light energy utilization efficiency, the carbon utilization efficiency and the moisture utilization efficiency; comprehensively considering the multivariable resistance-recovery state of the vegetation physiological process and the regulating variable of the resource utilization efficiency of the vegetation in response to drought stress, and analyzing the drought event resistance-recovery response capability of the vegetation. The method can more accurately and objectively understand the physiological mechanism difference of the vegetation to the drought stress response.

The invention relates to the technical field of plant stress resistance screening, in particular to a method for screening drought-resistant mutants after EMS mutagenesis of bamboo reed cluster buds, which comprises the following steps: S1, performing hydroponic seedling strengthening on wild tissue culture seedlings and EMS-treated rooting tissue culture seedlings to obtain wild bamboo reed seedlings and EMS-treated bamboo reed seedlings; s2, screening suitable concentration of PEG-6000: screening wild type bamboo reed seedlings by using a series of PEG-6000 solutions with different concentrations, comparing survival rates of the bamboo reed seedlings under different treatments, and screening suitable concentration for simulating drought stress by a PEG-6000 water culture method; s3, simulating drought stress conditions by using the screened PEG-6000 solution with proper concentration as a permeable medium, and screening the EMS treated bamboo reed seedlings to obtain drought-resistant plants. The screening method provided by the invention is not limited by seasons, has high accuracy, and is a simple, convenient and rapid method for screening drought-resistant bamboo reed plants.

The invention discloses an application of an msa-miR156e gene regulated by NO in regulation of drought resistance of plants, and is characterized in that a mature sequence of the msa-miR156e gene is as shown in SEQ ID No. 1, and a precursor sequence of the msa-miR156e gene is as shown in SEQ ID No. 2. The invention also discloses an application of the msa-miR156e gene in regulation of drought resistance of plants. According to the application, the mechanism of NO for regulating the alfalfa msa-miR156e gene to cope with drought stress is studied, the NO is found to up-regulate the expression of msa-miR156e, enhance the activity of antioxidant enzyme and reduce the accumulation of active oxygen, so that the drought resistance of alfalfa is remarkably improved, SPL family genes are regulated in a targeted manner through msa-miR156e, the physiological adaptability of plants is promoted, and the theoretical basis of plant stress resistance is enriched; the cultivation of a high-quality alfalfa variety adapting to a drought environment is facilitated.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

The invention discloses a gene DobHLH14 for regulating and controlling DoSUS2 expression under drought stress to increase the content of dendrobium officinale polysaccharides and application of the gene DobHLH14, and belongs to the technical field of plant genetic engineering breeding, the nucleotide sequence of the gene DobHLH14 is shown as SEQ ID No: 4, and the coded amino acid sequence is shown as SEQ ID No: 5. The DobHLH14 is applied to the improvement of the polysaccharide content of the plant or the breeding of the drought-tolerant transgenic plant. The homologous and heterologous overexpression results of the dendrobium officinale show that the polysaccharide content and drought resistance of the transgenic plant are obviously improved by regulating the expression of the DoSUS2 gene through the DobHLH14 transcription factor of the dendrobium officinale under drought stress, and the method is an excellent method for breeding perennial herbaceous plants with high polysaccharide and drought resistance.

The invention relates to cloning of a stress-resistant gene LpbHLH144 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH144 of Lilium pumilum DC. Comprising the following steps: (1) cloning a target gene; (2) constructing an expression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH144 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the functions of overexpression of the LpbHLH144 for improving the saline-alkaline and drought stress of the tobacco are identified through transgenic tobacco, the functions of the lilium tenuifolium bHLH gene when the lilium tenuifolium bHLH gene is subjected to adversity stress are disclosed, a foundation is laid for further application of the lilium tenuifolium in lily breeding, and a theoretical basis is provided for the functions and effects of bHLH transcription factors.

The invention relates to the technical field of carbon flux monitoring, and particularly discloses a carbon flux monitoring method and system based on soil drought remote sensing data, and the method comprises the steps: respectively collecting soil humidity remote sensing data and a soil vegetation coverage remote sensing image through a microwave sensor and an optical remote sensing sensor; performing resampling on the soil humidity remote sensing data and the soil vegetation coverage remote sensing image to realize spatial resolution alignment, and performing spatial distribution feature extraction on the soil humidity remote sensing data and the soil vegetation coverage remote sensing image after spatial alignment by adopting a deep learning algorithm; and pixel-level significance enhancement and fusion processing are carried out on the captured soil humidity distribution characteristics and vegetation distribution characteristics, so that nonlinear modeling of a dynamic influence mechanism of the soil drought degree on the vegetation growth state is realized, and intelligent estimation of the carbon flux is realized on the basis. In this way, the nonlinear regulation and control effect of soil drought stress on vegetation respiration and photosynthesis can be effectively described, and the efficiency and coverage range of carbon flux monitoring are improved.

A remote inspection vehicle for inspecting a plant for early drought stress and a neural network model for calculating a score corresponding to a level of early drought stress in the plant. The inspection vehicle may be equipped with an inspection camera having a radiometric infrared sensor and a red, green, and blue light sensor to capture plant images. Image data from the inspection vehicle, as well as soil moisture and calculated CWSI scores, can be used to train the neural network model, deriving weights and biases therefrom using a plurality of neural network layers. Using a plant canopy temperature value, a red raster value, a green raster value, a blue raster value, and a soil moisture value, the trained neural network model can generate a score which can be used to assess the level of early drought stress in a plant.

The invention relates to a crop drought-resistant agent, a method for improving crop drought resistance and / or promoting crop growth and development and application of the crop drought-resistant agent, and belongs to the technical field of agricultural production. The crop drought-resistant agent is prepared from the following components: arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water. The crop drought-resistant agent can promote normal growth and development of crops under drought conditions, improve plant biomass accumulation and cell antioxidant enzyme activity and reduce toxicity of ROS to the plants, so that adaptability, tolerance and resistance of the crops to drought stress are improved, and the crop drought-resistant agent has the advantages of being good in drought-resistant effect, high in stability and high in safety.

The invention discloses application of a corn ZmTST1 gene in improvement of plant yield and stress resistance. The nucleotide sequence of the corn ZmTST1 gene is as shown in SEQ ID NO. 1; the stress resistance is tolerance to salt stress and drought stress. Through sequence analysis and functional verification, after the ZmTST1 gene is over-expressed, the growth of plants can be promoted, the yield of the plants can be increased, meanwhile, the drought tolerance and the salt tolerance of ZmTST1 expressed plants are obviously higher than those of wild plants, and it is proved that the ZmTST1 gene has a remarkable effect in the aspects of high yield and stress resistance. By identifying the ZmTST1 gene, plants with high stress resistance can be screened, the cultivation period of the plants can be shortened, and a new method and thought are provided for plant breeding in the future.

The invention belongs to the technical field of natural disasters, and discloses a method for evaluating the toughness of a social economic system under drought stress. The method comprises the following steps: collecting monthly rainfall data and potential evapotranspiration data in a region, and calculating a meteorological drought index SPEI under the scale of three months; collecting social and economic data such as runoff data, crop evapotranspiration, industrial added value, population and the like of the region, estimating the available water amount of the region and the water demand in three aspects of agriculture, industry and life, and calculating a social and economic drought index; extracting the recovery time of the historical social economic drought event; calculating the cumulative intensity of meteorological drought during the historical social economic drought event; and fitting the drought recovery time and the accumulated drought intensity, and constructing a toughness curve of the social economic system under drought stress. According to the technical scheme, the drought toughness of the social economic system can be objectively and quantitatively evaluated.

The invention provides a wetland forest and grass ecosystem restoration strength evaluation method under drought stress. The method comprises the steps of data collection, daily-scale ecosystem service function index ESS construction, ecological drought identification and restoration elasticity curve construction. Wherein the time resolution of the index is accurate to the day, the capture of short-time drought below the monthly scale is enhanced, and the accurate description of the dynamic process of ecological system restoration under the drought stress is realized; combined identification is carried out on the drought events in combination with the standardized ecological water deficit, and the accuracy of identification of the drought events causing significant differences of ecological system service functions is improved; a quantile regression model is utilized to quantify the ecological system recovery capability of different vegetation types, and data characteristics of different recovery time distributions of the ecological system under the same drought intensity are considered, so that the defect that a traditional mean value regression model cannot fully describe drought complex characteristics is overcome; and a theoretical support is provided for sustainable development of an ecological system under an extreme climate condition.

The invention discloses a rain-fed agricultural area drought index construction and application method, equipment and a medium. The rain-fed agricultural area drought index construction and application method comprises the following steps: S1, determining drought influence coefficients of crops in different growth stages; s2, constructing an APSIM model, and carrying out parameter calibration and model verification; s3, the APSIM model dynamically simulates the phenological period, the yield and soil water in the growing season of the crops in the sufficient irrigation and rain culture mode; s4, calculating the crop yield reduction rate; s5, constructing a drought stress index relational expression including the cumulative drought stress degree, the cumulative drought stress index and the standardized cumulative drought stress index; s6, based on the dynamic simulation result of the APSIM model, the crop yield reduction rate and the accumulated drought stress index are calculated in a fitting mode. According to the method, the soil humidity data acquisition problem is considered, the sensitivity difference of different growth stages to drought is also considered, and accurate quantitative evaluation of the drought stress response in the whole crop growth process can be further realized.

The invention belongs to the technical field of gene engineering, and provides a drought-resistant gene OsAP4, a protein coded by the same and application of the drought-resistant gene OsAP4. According to the invention, after the OsAP4 gene is edited in rice, the plant shows drought tolerance under the drought stress condition simulated by PEG6000 treatment; and after the OsAP4 gene is over-expressed in the rice, the drought tolerance of the plant is reduced, which indicates that the drought tolerance of the rice can be regulated and controlled by a method for changing the gene function. An editing carrier containing an OsAP4 editing site is transferred into rice, and the drought tolerance of the rice can be improved by editing the gene; the OsAP4 gene can also be used as a molecular marker to be applied to molecular breeding. The OsAP4 gene has a huge application prospect in molecular breeding and cultivation of drought-resistant rice by utilizing genetic engineering means.

The invention discloses application of a transcription factor BnaMYB52 in regulation and control of drought resistance of rape, and relates to the technical field of biology. The amino acid sequence of the transcription factor BnaMYB52 is as shown in any one of SEQ ID NO. 5 to 8. According to the invention, the negative regulation effect of the transcription factor BnaMYB52 in the drought resistance of the rape is disclosed for the first time, and a brand new molecular target and a genetic improvement strategy are provided for improving the drought resistance of the rape. Through in-depth study, it is found that knockout of the BnaMYB52 gene can significantly enhance the tolerance of rape plants to the drought environment. In addition, a new drought-resistant rape germplasm obtained after genetic modification of the gene shows excellent adaptive capacity in a drought stress environment. The technical breakthrough not only helps to solve practical problems faced by rape planting in arid regions, but also lays a solid theoretical foundation and technical support for cultivating high-yield and high-quality rape varieties adapting to extreme climate conditions.

The invention provides application of an OsRTD1 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsRTD1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the OsRTD1 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of the OsRTD1 gene mutant plant osrtd1 under the drought stress of PEG induction and soil dehydration is remarkably improved, the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced, the relative water loss rate of dehydrated leaves is higher, and the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced. The total grain number, the solid grain number, the total grain weight and the like of the OsRTD1 gene mutant plant osrtd1 under the stress of field drought are obviously higher than those of a wild plant. The OsRTD1 gene is a potential candidate new target for regulating and controlling a rice drought resistance mechanism.

The invention provides a tree species screening method for high-altitude drought afforestation, an afforestation method and application, and belongs to the technical field of forest tree breeding and cultivation. According to the screening method, five physiological and biochemical indexes of soluble protein, malondialdehyde, proline, peroxidase and soluble sugar in the tree species are measured through a gradient drought stress experiment, and comprehensive scoring is performed by adopting a membership function, so that the drought-resistant tree species are scientifically screened. The tree species such as sophora moorcroftiana, rosa serrulata and amorpha fruticosa screened by the method are applied to afforestation in the high-altitude arid region, and the afforestation survival rate and preservation rate can be remarkably increased. The invention provides a reliable technical scheme for solving the long-term problem that the selection of tree species in the difficult site field lacks scientific basis.

The invention relates to the technical field of gene engineering, in particular to application of a rice OsSAUR4 gene in regulation and control of rice drought tolerance, and the sequence of the rice OsSAUR4 gene is selected from any one of the following: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a protein sequence as shown in SEQ ID NO.2, and 3) a sequence of which the homology with the nucleotide sequence as shown in SEQ ID NO.1 is more than 90%. The rice OsSAUR4 gene is knocked out, so that the drought tolerance of rice is improved, and the survival rate of rice in a drought stress environment is improved.

The invention discloses a method for improving drought tolerance and yield of wheat through combined application of compost and sodium selenite, and belongs to the technical field of agricultural planting. According to the method, wheat is planted after compost is applied to soil, and when drought stress occurs, a sodium selenite solution is sprayed to wheat leaves, so that oxidative stress indexes such as MDA, H2O2 and O2 <-> of wheat can be remarkably reduced, and the activity of antioxidant enzymes such as SOD, POD, CAT and APX can be improved; physiological indexes such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, protein, soluble sugar and proline of wheat leaves are improved, growth and yield related traits of the wheat are improved, and finally the drought resistance and yield of the wheat are improved. The compost is prepared by taking 50%-55% of corn straw, 10%-20% of flue-cured tobacco straw, 20%-25% of rice straw, 10%-15% of livestock manure and 0.1%-1% of bacillus subtilis as raw materials, adding organic wastewater or tap water, mixing, composting and fermenting.