460 results about "Drought stress" patented technology

The invention belongs to the technical field of drought risk prediction and vegetation influence evaluation, and particularly discloses a vegetation-to-drought resource utilization coupling response strategy evaluation method, which comprises the following steps: acquiring target data; constructing a drought index according to the target data, and extracting a drought event; extracting vegetation total productivity, vegetation net level productivity and evapotranspiration abnormal data before and after the drought event, and calculating response intensity of vegetation variables; calculating the average variation of the light energy utilization efficiency, the carbon utilization efficiency and the moisture utilization efficiency; comprehensively considering the multivariable resistance-recovery state of the vegetation physiological process and the regulating variable of the resource utilization efficiency of the vegetation in response to drought stress, and analyzing the drought event resistance-recovery response capability of the vegetation. The method can more accurately and objectively understand the physiological mechanism difference of the vegetation to the drought stress response.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention provides application of an OsRTD1 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsRTD1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the OsRTD1 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of the OsRTD1 gene mutant plant osrtd1 under the drought stress of PEG induction and soil dehydration is remarkably improved, the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced, the relative water loss rate of dehydrated leaves is higher, and the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced. The total grain number, the solid grain number, the total grain weight and the like of the OsRTD1 gene mutant plant osrtd1 under the stress of field drought are obviously higher than those of a wild plant. The OsRTD1 gene is a potential candidate new target for regulating and controlling a rice drought resistance mechanism.

The invention provides a tree species screening method for high-altitude drought afforestation, an afforestation method and application, and belongs to the technical field of forest tree breeding and cultivation. According to the screening method, five physiological and biochemical indexes of soluble protein, malondialdehyde, proline, peroxidase and soluble sugar in the tree species are measured through a gradient drought stress experiment, and comprehensive scoring is performed by adopting a membership function, so that the drought-resistant tree species are scientifically screened. The tree species such as sophora moorcroftiana, rosa serrulata and amorpha fruticosa screened by the method are applied to afforestation in the high-altitude arid region, and the afforestation survival rate and preservation rate can be remarkably increased. The invention provides a reliable technical scheme for solving the long-term problem that the selection of tree species in the difficult site field lacks scientific basis.

The invention relates to the technical field of gene engineering, in particular to application of a rice OsSAUR4 gene in regulation and control of rice drought tolerance, and the sequence of the rice OsSAUR4 gene is selected from any one of the following: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a protein sequence as shown in SEQ ID NO.2, and 3) a sequence of which the homology with the nucleotide sequence as shown in SEQ ID NO.1 is more than 90%. The rice OsSAUR4 gene is knocked out, so that the drought tolerance of rice is improved, and the survival rate of rice in a drought stress environment is improved.

The invention relates to the field of plant molecular biology and forest genetic engineering, and particularly provides a PagRAP2.3 protein point mutant and application thereof in drought resistance of poplar. The mutant PagRAP2.3 MA is obtained by mutating methionine at the first site and cysteine at the second site of a wild type PagRAP2.3 protein into methionine and alanine. The invention further discloses a preparation method of the mutant PagRAP2.3. An expression vector containing the PagRAP2.3 MA gene is constructed, poplar 84K is transformed through an agrobacterium-mediated method, and a transgenic line with stable expression is obtained. Functional verification results show that overexpression of PagRAP2.3 MA can significantly increase the plant height of the poplar and promote plant growth, but the sensitivity to moisture is enhanced under drought stress. The mutant can be used for regulating and controlling the growth and development of forest trees and evaluating the drought resistance, and has a forestry breeding application prospect.

Disclosed are a Solanum tuberosum drought tolerance-related transcription factor gene StPIF4 and the use thereof. A CDS sequence of StPIF4 has a length of 1554 bp, has a nucleotide sequence shown as SEQ ID No. 1, and encodes 517 amino acids, the amino acid sequence being shown as SEQ ID No. 2. The gene is located in a cell nucleus. The gene reduces the water loss of Solanum tuberosum seedlings under the drought stress by adjusting the opening degree of stomas, thus enhancing the drought tolerance of Solanum tuberosum. The present invention provides a new genetic material and a theoretical basis for analyzing the drought-tolerant molecular mechanism and drought-tolerant breeding improvement of Solanum tuberosum.

The invention relates to the technical field of biological genetic engineering, in particular to application of a brassica napus BnaA01G0331000ZS gene in drought stress resistance. The BnaA01G0331000ZS gene is cloned by using cDNA of double 11 leaves as a template through a sequence published in a brassica napus genome database, an over-expression vector is constructed and genetically transformed into rape variety high oil for transgenic function verification, the drought resistance of the obtained over-expression transgenic line is obviously enhanced compared with that of a wild type, and the BnaA01G0331000ZS gene has the advantages that the BnaA01G0331000ZS gene is obtained; therefore, the gene can positively regulate the drought stress resistance of the rape. Therefore, the invention provides a new gene resource for adversity stress resistance of rape.

The invention provides an application of a sunflower HaSnRK2 gene in regulating and controlling salt tolerance and drought tolerance of plants, and belongs to the technical field of plant genetic engineering. The invention provides an overexpression vector of a sunflower HaSnRK2 gene. The overexpression vector is applied to regulation and control of salt tolerance and drought tolerance of plants after genetic transformation is carried out on the plants by using a flower dipping method. Through overexpression of the sunflower HaSnRK2 gene, the salt stress tolerance and the drought stress tolerance of plants are remarkably enhanced, and damage caused by reactive oxygen free radicals induced by salt stress and drought stress can be effectively relieved.

The invention provides application of an OsERF8 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsERF8 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsERF8 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of an oserf8 mutant plant under PEG induction and soil dehydration drought stress is remarkably improved; and the survival rate of the overexpressed plant OsERF8-OE under the induction of PEG (Polyethylene Glycol) is obviously reduced. Compared with a wild type plant, after PEG induces drought stress, the malondialdehyde content of the overground part of the oserf8 mutant plant is reduced, and the proline content is increased; the malondialdehyde content of the overground part of the overexpressed plant OsERF8-OE is increased, and the proline content is reduced. In conclusion, the OsERF8 gene is a potential candidate new target for researching a rice drought resistance regulation mechanism.

The invention discloses application of a watermelon WRKY transcription factor ClWRKY60 in drought stress resistance, and belongs to the technical field of gene engineering. The invention discloses an application of a watermelon WRKY transcription factor ClWRKY60 in drought stress resistance. The nucleotide sequence of the watermelon WRKY transcription factor ClWRKY60 is as shown in SEQ ID NO. 1. The WRKY transcription factor ClWRKY60 is a positive regulation factor of watermelon responding to drought stress, the gene can provide gene resources for creation of drought-resistant new germplasm and breeding of special high-resistance new varieties, and meanwhile, the gene has very important scientific and production application significance for promoting resistance breeding, improving economic benefits of watermelons and the like.

The invention discloses application and a method of wheat demethylase TaALKBH23 in regulation and control of drought stress resistance of plants, a coding sequence of the demethylase TaALKBH23 is shown as SEQ ID NO: 1, and an amino acid sequence coded by the demethylase TaALKBH23 is shown as SEQ ID NO: 2. The invention provides demethylase TaALKBH23 and a function and application of a coding gene of the demethylase TaALKBH23 in regulation and control of plant drought resistance, and it is found through construction of a TaALKBH23 wheat genetic transformation material that the gene negatively regulates and controls wheat drought resistance. It is found that TaALKBH23 plays an important role in the process of regulating and controlling drought stress resistance of plants, and a new gene resource is provided for improving drought resistance of crops.

The invention belongs to the technical field of agricultural biology, and provides a soybean drought stress tolerance gene and application thereof, and the nucleotide sequence of the gene comprises a CDS sequence as shown in SEQ ID NO: 1; the nucleotide sequence has at least 90% of homology with the sequence shown in SEQ ID NO: 1 and is used for coding a protein with a drought stress response function; one or more nucleotides are substituted, deleted or inserted in the sequence as shown in SEQ ID NO: 1, and a nucleotide sequence of a protein with a drought stress response function is coded; according to the application disclosed by the invention, the direct association between the soybean gene Gm12g076200 and the drought stress tolerance is clarified, and the deletion of the soybean gene Gm12g076200 causes a soybean drought sensitive phenotype, so that the research blank of the gene on the stress response function is filled; the deletion of the gene can significantly reduce the tolerance of the soybean to drought stress, and the expression quantity is significantly increased under the drought stress, so that a key candidate gene is provided for research of a soybean drought-resistant molecular mechanism.

The invention relates to the technical field of ridge construction, and discloses an ecological ridge construction method for slowing down lateral seepage and intercepting non-point source pollution, which comprises the following steps of: extracting soil texture, elevation and area information of a target area, and scientifically delimiting a ridge and a farmland boundary by combining a spatial analysis and function partitioning algorithm; dynamically determining the height of the buffer zone according to the height difference relation and the flood and drought stress characteristics of plants; hydraulics characteristics are obtained through hydrological simulation, the width of the buffer zone and the filler amount are calculated based on the length-width ratio and hydrodynamic force coupling mechanism, optimal design of water flow regulation and control and pollution interception is achieved, and the stability and purification efficiency of the ridge system in different environments are ensured. According to the invention, the ecological ridge system with strong adaptability is constructed through cooperative analysis of multi-source terrain environment and ecological parameters.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention belongs to the technical field of gene engineering, and particularly relates to application of a GhHAT18 gene in regulation and control of drought stress resistance of plants, the nucleotide sequence of the GhHAT18 gene is shown as SEQ ID NO.1. The invention discloses that the cotton histone acetylase gene GhHAT18 can be used for regulating and controlling the drought resistance of the plants for the first time, and experiments show that compared with a wild type plant, after the expression of the GhHAT18 gene is inhibited, the GhHAT18 gene can be used for regulating and controlling the drought stress resistance of the plants. The antioxidant enzyme activity and the drought resistance of plants can be obviously reduced; and the transgenic plant over-expressed with the GhHAT18 shows stronger and higher antioxidant enzyme activity and drought resistance under the drought stress. Transcriptome analysis shows that the GhHAT18 gene affects the expression of drought-resistant related genes through a secondary metabolite synthesis pathway and an MAPK signal transduction pathway, and the adaptive capacity of plants to drought stress is further regulated and controlled.

The invention discloses a transcription factor ZmbHLH180 gene as well as an encoded protein and application thereof, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the corn ZmbHLH180 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2. The invention provides a transcription factor ZmbHLH180 gene, the CDS full length of the gene is 864bp, 287 amino acids are encoded, the gene is mainly positioned in a cell nucleus, and the expression of a chlorophyll degradation gene NYC1 and the senescence of leaves can be obviously promoted by over-expressing the ZmbHLH180 gene in arabidopsis thaliana; the trans-ZmbHLH180 gene overexpressed corn can remarkably promote leaf greening under drought stress, a certain technical foundation is laid for regulation and control of the plant growth cycle, a choice is provided for genetic improvement of new plant varieties, and the application value is important.

The invention relates to the technical field of biology, in particular to a tobacco NtATC3 / 4 gene and application of the tobacco NtATC3 / 4 gene to regulation and control of plant flowering and leaf surface area. The function of the NtATC3 / 4 gene is researched by using a virus induced gene silencing (VIGS) technology, and the result shows that after the NtATC3 / 4 gene is silenced, the flowering time of tobacco can be delayed, the leaf surface area can be increased and / or the stem length can be reduced. The results show that the flowering time of tobacco can be controlled by regulating and controlling the expression quantity of the NtATC3 / 4 gene, so that early flowering is prevented and controlled, the yield reduction caused by cold and drought stress is reduced, the leaf surface area is increased, and the income of tobacco growers is increased, and therefore, the NtATC3 / 4 gene has a wide market application prospect.

The invention discloses a banana drought-enduring functional gene MaPUB21 and application of the banana drought-enduring functional gene MaPUB21 in improvement of plant drought-enduring capability. A nucleotide sequence of the gene is shown as SEQ ID NO.2. The invention further discloses a method for preparing the banana drought-enduring functional gene MaPUB21. According to the invention, a silence vector (MaPUB21-pTRV) aiming at the MaPUB21 gene is constructed, and an agrobacterium-mediated method is utilized to carry out instantaneous silence in the Musa communis. Drought tolerance analysis shows that after drought stress treatment, compared with a wild type, the content of malonaldehyde, superoxide anions and hydrogen peroxide of the MaPUB21 silent strain is remarkably reduced, and the content of proline and the activity of peroxidase are remarkably improved. The result shows that the drought tolerance of the plant can be effectively improved by silencing the MaPUB21 gene. The gene provided by the invention provides a new resource for plant stress-resistant molecular breeding, and is of great significance for cultivating drought-resistant plants and improving the utilization rate of drought land.

The invention discloses application of an exogenous regulating substance in relieving corn drought stress, and belongs to the technical field of biology. It is verified that external application of serine, pantothenic acid or raffinose can promote root morphology establishment of corn under drought stress, enhance antioxidant enzyme activity of corn roots, reduce MDA accumulation and increase soluble protein content, 0.6 mM of serine, 0.9 mM of pantothenic acid and 1.0 mM of raffinose can effectively relieve stress of the drought environment on corn, and the corn drought resistance can be effectively improved. The water retention capacity of corn is improved, the antioxidant enzyme activity of corn roots is enhanced, the content of osmotic regulation substances is regulated and controlled, damage of drought stress to corn cells is reduced, and meanwhile growth of corn plants and establishment of root morphology are promoted. The invention provides a new strategy for drought-resistant cultivation of corn.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention belongs to the technical field of plant genetic engineering and molecular breeding, and particularly relates to application of a soybean GmUGT72E1 gene in regulation and control of soybean plant height and enhancement of resistance of soybeans to drought and alkali stress. Plant height is a key agronomic character influencing lodging resistance and photosynthetic efficiency of soybeans, drought and alkali stress are core abiotic stress factors restricting yield and quality of the soybeans, and the three factors jointly lead to remarkable global soybean yield reduction loss. Gene cloning, vector construction and transgenic function verification prove that overexpression of the GmUGT72E1 gene can reduce the plant height of soybeans by 15%-25%, meanwhile, the tolerance of the soybeans to drought and alkali stress is remarkably improved, the relative water content of leaves under the drought stress is increased by 25%-30% compared with that of wild type leaves, and chlorophyll under the alkali stress is increased by 40%-50% compared with that of the wild type leaves. According to the application disclosed by the invention, the dual regulation and control functions of the GmUGT72E1 gene are defined, key gene resources and technical support are provided for soybean dwarf stress-resistant collaborative improvement, dwarf stress-resistant soybean varieties can be cultivated by means of transgenosis, gene editing and the like, and the application has important agricultural application value.

The present application relates to the technical field of genetic engineering, and particularly relates to application of overexpression of BnA05CARK3 gene in regulation of fertility of Brassica napus. The present application successfully constructs a recombinant vector for overexpression of BnA05CARK3 gene by using the cloned BnA05CARK3 gene, and makes the BnA05CARK3 gene in Brassica napus to be genetically transformed and overexpressed by means of agrobacterium infection, and then promotes the development process of Brassica napus under drought stress environment, so that the pollen vitality and the thousand-grain weight of Brassica napus can be improved, and the quality and yield can reach the normal development degree of wild type Brassica napus.

The invention provides a camellia oleifera seedling drought degree classification method and system based on multi-modal data, and belongs to the technical field of plant detection, the scheme is based on a ConvNeXt architecture, a graph convolutional network (GCN) and a dynamic fusion gate mechanism are combined, image data and root system data of camellia oleifera seedlings are effectively fused, fusion extraction of multi-modal data features is realized, and the method and the system have good application prospects. The classification accuracy of the drought degree of the camellia oleifera seedlings is effectively improved; according to the scheme, the spatial topology modeling capability of a leaf image is enhanced by introducing a graph convolutional network (GCN), the weights of the image and physiological features are adaptively adjusted in combination with a dynamic fusion gate, the weights of the image and root system features are adaptively adjusted through the dynamic fusion gate, the model can accurately capture complementary information among different modals, and the modeling efficiency is improved. And the accuracy of drought stress classification is remarkably improved.

The invention belongs to the technical field of biology, and particularly relates to Dendrobium officinale DoSTRK1 protein as well as a coding gene and application thereof. The invention discloses a Dendrobium officinale DoSTRK1 protein, which comprises a protein consisting of an amino acid sequence as shown in SEQ ID NO.6, and a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant drought tolerance and is derived from the protein. The invention finds that overexpression of the gene encoding the DoSTRK1 protein can enable an overexpression strain to show stronger tolerance under salt stress of a plant, and specifically, the overexpression strain shows longer roots in a seedling stage and higher chlorophyll content in a growth stage. Under drought stress, the overexpression strain also shows stronger tolerance, and compared with a wild type, the overexpression strain has the advantages that the root length in the seedling stage is increased, and the survival rate in the growth stage is higher.

The invention relates to the technical field of plant genetic engineering, in particular to a ZmbHLH166 gene related to drought resistance in the flowering stage of corn, an encoding protein and application of the ZmbHLH166 gene. The key effect of the ZmbHLH166 gene in the drought resistance of the corn in the flowering period is identified for the first time on the basis of whole genome association analysis (GWAS) and transgenic function verification, and through experiments, through genetic transformation and continuous selfing screening of a Ubi1: ZmbHLH166 overexpression vector, the obtained T2-generation corn strain of the overexpression ZmbHLH166 gene has the advantages that compared with a wild type corn strain, the yield is increased, and the yield is increased. The ZmbHLH166 gene has a shorter pollen scattering-spinning interval, which indicates that under drought stress, the overexpression of the ZmbHLH166 gene can effectively promote the synchronous development of male and female flowers of corn, thereby enhancing the drought resistance of crops. Therefore, the application of the ZmbHLH166 gene and the protein coded by the ZmbHLH166 gene in regulating and controlling the drought resistance of the corn in the flowering period is feasible, and a new and reliable gene resource and an improvement direction are provided for drought-resistant breeding of the corn.

The application belongs to the technical field of genetic engineering and relates to TaGoLS10 application of a gene and an encoded protein in regulating drought resistance of plants. TaGoLS10 The gene can positively regulate the drought resistance phenotype of wheat, and the TaGoLS10 protein encoded by the gene can specifically interact with a drought resistance related protein TaGTs, and both of them cooperatively participate in and regulate the response process of wheat to drought stress, thereby enhancing the drought resistance of wheat. The application adopts a technical strategy combining overexpression technology and RNAi technology, and successfully creates new wheat materials with significantly improved or reduced drought resistance.

This invention belongs to the field of genetic engineering, specifically involving LbMYB88 Gene or LbMYB88 Application of gene overexpression vectors in improving plant resistance to drought stress. This invention utilizes a newly created... LbMYB88 Overexpression of the gene in wolfberry plants was observed after drought treatment. LbMYB88 The overexpression of the gene in wolfberry plants showed significant resistance compared to the wild type, providing genetic resources for breeding stress-resistant wolfberry varieties and possessing good potential application value. It also lays a theoretical foundation for studying the molecular mechanism of drought resistance in wolfberry.

The application discloses a MaDREB1F gene, and a nucleotide sequence of the MaDREB1F gene is shown as SEQ ID NO:1. Experimental research results reveal a mechanism of MaDREB1F in mediating non-biological stress resistance of bananas, and determine new targets (MaAOC4, MaACO20 and MaERF11) of MaDREB1F, which are beneficial to design breeding strategies to improve the resistance of crops to cold and drought stress.

The invention discloses application of a CtHCT061 gene in cultivation of a new high-lignin drought-tolerant safflower strain, and research finds that the safflower CtHCT061 gene has the highest expression quantity in the root of safflower, and then in the stem of safflower; a result of constructing the expression vector shows that under drought stress treatment, the MDA content and the H2O2 content in the transgenic pCAMBIA3301-CtHCT061 safflower hairy roots are remarkably reduced compared with those of a control group strain, and the proline content is remarkably increased. Under the drought stress treatment, CtHCT061 overexpression can up-regulate the lignin content in the safflower, alleviate oxidation and membrane damage of the safflower under the drought stress, and improve the tolerance of the safflower to the drought stress.