716 results about "Drought stress" patented technology

The invention discloses an agricultural drought grade monitoring method based on a temperature vegetation drought index (TVDI). The method comprises the following steps of: 1, data preparation; 2, land surface temperature (LST) data reconstruction; 3, construction of crop plantation area normalized difference vegetation index-land surface temperature (NDVI-LST) feature space; 4, TVDI calculation; and 5, drought grade monitoring based on the TVDI. The invention brings forward an LST data reconstruction method based on multi-year background values and area fluctuation values, a cultivated land area multi-year NDVI-LST feature space is constructed for crops, the TVDI is calculated, a crop drought grade monitoring model based on a supervision classification idea is designed for drought grade remote sensing monitoring, the model can quite accurately reflect drought threat degrees of the crops under difference conditions in real time, and the method has great significance in monitoring, early warning and prevention of agricultural disasters.

The invention discloses a plant growth matrix with high water retention for spray seeding revegetation and a preparation method thereof. The plant growth matrix comprises an upper layer and a lower layer, wherein the lower layer is a plant growth matrix layer, and the upper layer is an organic matter covering layer covered on the plant growth matrix layer. According to the invention, perlite and montmorillonite with high hydroscopicity are applied to modification of the matrix for spray seeding revegetation, an organic matter is used to cover the surface of the plant growth matrix, a preparation process for the matrix is optimized, and thus, the disadvantage that moisture in a common matrix easily volatilizes is improved; the matrix for spray seeding revegetation has good adhesion, can be firmly attached on the surface of a bare rock side slope, well absorbs and maintains moisture, uninterruptedly provides moisture and nutrient elements needed by plant growth, guarantees revegetation of the side slope and has the advantages of low cost and easiness in manufacturing. Through implementation of a technical scheme in the invention, it is overcome that vegetation easily dies from drought after spray seeding of nonlocal soil on a side slope in an arid rainless region in China.

The invention discloses a method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi to bring the reciprocal symbiosis relation between AM fungi and host plants under drought stress into play reasonably and efficiently. According to the method, the AM fungi having a close symbiotic relation with the wheat are selectively used, and the symbiotic relation between the AM fungi and the wheat is built in a condition control mode to promote the wheat growth and improve drought adaptability. The AM fungi comprise one of glomus monosporum and acaulospora laevis, the two kinds of AM fungi are respectively propagated in an expanding mode in an expanding propagation matrix by using red clovers, and then two AM fungi inoculants and the wheat are cultured together in a symbiotic culture medium. The effective path for profitable symbiosis of the AM fungi and the host plants in adversity conditions is provided, the infection and symbiosis relation with the host plants of the AM fungi is improved in a man-made mode, and the yield of dry crops is improved.

The invention discloses a method for identifying drought resistance of corns. The method for identifying drought resistance of corns is to alternatively plant the corns in a water area and an arid area, namely a film water area (2 rows) and a film arid area (2 rows), the same material is planted in adjacent water and arid areas, and the method belongs to the technical field of corn planting. The method comprises the following steps of (1) sowing the corn seeds to be tested; (2) finally singling the corn plants to be tested; (3) conducting drought stress and normal water treatment on the corn plants to be tested; (4) conducting morphological index detection on the corns to be tested; (5) conducting yield index detection on the corns to be tested; (6) evaluating the drought resistance of the corns to be tested. A method for identifying drought resistance of corns in adult stage is established, adult corn plants are taken as detecting objects in the method, drought stress treatment is conducted on the corn from jointing stage to mature stage, through the detection of the plant morphological index and yield index, the purpose of establishing a subjective and accurate method for identifying and evaluating the drought resistance of the corns is achieved, and therefore a reliable basis for the identification of drought resistance of corns is provided.

The invention relates to a wheat CBL-CIPK (CBL-interacting protein kinase) stress tolerance regulatory factor as well as an encoding gene and an application thereof. According to the wheat CBL-CIPK stress tolerance regulatory factor, protein kinase genes which are consciously induced by salt and drought when being expressed in roots and leaves are selected according to expression profile chip information of salt and drought stress of wheat, and a cDNA (complementary deoxyribonucleic acid) sequence with a whole-length encoding area is cloned, wherein the nucleotide sequence is as shown in SEQ ID NO.1. Analysis shows that the gene can encode CBL-CIPK serine/threonine protein kinase, the amino acid sequence is as shown in SEQ ID NO.2, and is named as TaCIPK33. Transgenic function analysis shows that the gene has the functions of improving plant salt tolerance and drought resistance. Basis is provided for further understanding molecular mechanisms of wheat in responding to drought and saltstress, and meanwhile the gene can be applied to genetic improvement of novel stress tolerance germplasms of crops such as wheat.

The invention relates to a method for improving the drought resistance of drought substrate tall fescue by adopting urban household fine garbage compost, which comprises the following steps of: respectively scattering fine garbage compost of which the particle diameter is respectively 1200nm, 600nm and 300nm uniformly on the surface of soil, wherein the ratio of the particle diameter to parts by weight of the soil is 1:60-80, the temperature is controlled at 20-26 DEG C, the relative humidity is controlled at 40-60%, and natural light permeating a room is used for illuminating; fully watering at the initial stage of seeding; weighing and watering every one or two days according to 55-65% of field moisture capacity after two weeks to maintain the field moisture capacity in the stress range; and measuring each index from the 44th day. The result shows that under the drought stress, the fine compost increases the biomass of the tall fescue to a certain extent, and the research result of growth of root systems of lawn plants can be basically met by matching the compost with the substrate for cultivating turf.

The invention relates to a method for irrigating rice, which is a comprehensive technology for the water and fertilizer regulation of a rice field wetland system in rainy areas in the southern of China. Based on the agricultural irrigation and rainfall coordination idea, field water and fertilizer conversion and utilization are used as a system to be considered, rice is subjected to appropriate drought stress before irrigation under the condition that the yield of crops is not reduced, the rice is subjected to appropriate waterlogging stress when suffering from large rainfall, and the droughtstress and the waterlogging stress alternately happen, thus, the water and fertilizer contents of a rice field are scientifically adjusted, multiple arms of ensuring that the yield of regional rice is not reduced, increasing the utilization ratio of rainwater, reducing the water consumption for irrigation, reducing flood disasters, reducing water lifting energy consumption of a pump station, increasing the utilization efficiency of fertilizer and improving the regional water environment are achieved; and the comprehensive benefit of the rice field wetland system is exerted to the maximum extent.

The invention relates to a cloning and function expression method of an adversity stress AhMYBL6 gene in peanut. The adversity stress AhMYBL6 gene is synthesized by RT-PCR (reverse transcription-polymerase chain reaction) cloning by the steps of preparation and treatment of materials, extraction of RNA (ribonucleic acid) and synthesis of cDNA (complementary deoxyribonucleic acid). The open reading frame of the gene is 861bp, and 287 amino acids are coded totally. The homology of the amino acid sequence of the gene with the MYb family proteins GmMYB4, GmMYB29 and GmMYB29A2 of the soybean is higher than 60%. The fluorescent quantitative PCR verifies the expression patterns of the AhMYBL6 under low temperature, salt stress and drought stress; the result indicates that the expression levels of the AhMYBL6 are obviously enhanced after various stress treatment for 1 hour, and kept at high levels all the time thereafter; and the expression level of the AhMYBL6 in the leaf subjected to low-temperature treatment is maximally enhanced by 20 times. After the gene is transferred into peanut by transgenic means, compared with the control, the transgenic plant has obvious characteristics of cold tolerance, salt tolerance and drought tolerance. The AhMYBL6 gene can obviously enhance the stress tolerance of the peanut.

The invention provides Gingko MADS-box transcription factor gene GbMADS9 for controlling blossoming of plants; the gene is acquired by subjecting cDNA as a template to PCR (polymerase chain reaction) amplification, wherein the cDNA is acquired by reverse transcription of total RNA of gingko female flowers. The gene has the advantages that the gene GbMADS9 is capable of controlling blossoming of plants and shortening juvenile phase of plants, and under induction of GA3, ABA, salt stress, drought stress and cold damage stress, the overexpressed gene GbMADS9 is capable of enhancing plant tolerance to osmotic stress; the gene is applicable to controlling the blossoming phase of plants.

The invention discloses a compound preparation for relieving corn drought stress as well as a preparation method and application thereof, belonging to the technical field of crop stress-resistant preparation. The compound preparation disclosed by the invention is classified into a seed coating agent and a plant spray agent and contains the components such as trehalose glycolipid, betaine and calcium superphosphate; and the components are uniformly mixed and stirred according to certain proportion. After coating treatment of the seeds according to a suitable proportion, the seed germination can be effectively promoted, the seedling vigor is enhanced, the overall water regime of corn plants under drought stress is relieved, the damage degree of the plants is lightened, and the growth and development of the plants are adjusted so as to improve the corn quality and yield. By adopting the plant spray agent, a little effect of sprinkling irrigation water supply also can be realized; and the trehalose glycolipid also can realize certain inhibitory effect on the pathogenic bacteria of leaves.

The invention relates to the technical field of plant genetic engineering, in particular to a gene for regulating and controlling the corn drought stress resistance. The gene is a ZmPP2CA10 deletion mutation gene; the deletion segment is a continuous base sequence with a distance being 301bp away from the upstream of the ZmPP2CA10 initial codon ATG; the continuous basic group sequence is shown as SEQ ID NO:1; the nucleotide sequence of the mutated ZmPP2CA10 promoter is shown as SEQ ID NO:2. The deletion mutation gene can be used for corn drought resistance relevant molecular marker auxiliary breeding and corn drought resistance auxiliary identification; the operation is convenient; the accuracy rate is high.

A method for using the promotor LEAP of rice's drought inducting gene to improve the resistance of plant to drought features that the promotor LEAP of rice OsLEAl gene is cloned. Its nucleotide sequence is shown by SEQ ID No.1 and is used to code a drought induced expression protein. Two cis-acting elements ABRE contained in its promotor region can be specifically response to drought and ABA. Its inductive expression carrier can stongly induce the expression of target gene when the drought stress is applied to a plant. The method for culturing the drought-resistant plant is also disclosed.

The invention discloses a deep-layer infiltration method for apple orchards at dry highland areas through rainwater collected by tree disks. The deep-layer infiltration method comprises the following steps of selecting orchards; making deep-layer infiltration buckets for the collected by rainwater; trimming tree disks; burying the rainwater collection deep-layer infiltration buckets; and covering black mulching films. The deep-layer infiltration method disclosed by the invention has the beneficial effects that in the deep-layer infiltration method for the apple orchards at the dry highland areas through the rainwater collected by the tree disks, the black mulching films are covered, so that the water content of soil is effectively increased, the spring temperature and the summer temperature of the soil are effectively increased, and the microenvironment of the soil is effectively improved; the infiltration buckets of 40cm are buried, so that the water content of the soil below root layers is increased, the physical properties of the soil are changed, the growing environment of root systems is improved, water consumed for the growth of fruit trees in winter and spring drought seasons are compensated, drought stress on apple trees is small, the yield of a single plant is increased, the quality of 100 leaves of the apple trees is increased, the ratio of new shoots to short shoots is increased, the shape index of the fruit trees is increased, and the appearance of on-year and off-year of the apple trees is effectively prevented; and besides, water in rainy seasons can be effectively accumulated into deep layers, the trans-seasonal utilization of precipitation is realized, and the collected rainwater can also be used as a water soluble fertilizer for irrigation use.

The invention relates to a method for improving drought stress resistance of artificially restored Calligonum rubicundunm in a drought desert area, which comprises the following steps of nursery stock selection, early soil preparation, early nursery stock treatment, irrigation treatment and the like, and utilizes a feedback regulation system formed through drought induction of partial root systems of the Calligonum rubicundunm to improve the adaptive capacity of the Calligonum rubicundunm to the drought so as to achieve the aim of improving the integral drought stress resistance of the artificially restored Calligonum rubicundunm in the drought desert area and avoid converse succession after the artificial assistance measures are cancelled. The method realizes survival and growing development of artificially restored Calligonum rubicundunm seedlings by means of the drought stress resistance of the Calligonum rubicundunm seedlings under the natural conditions of precipitation amount less than 100 mm and drought climate after the artificial assistance measures are cancelled.

The invention relates to and aims at providing a rice RING finge family E3 ubiquitin ligase OsDHS gene as well as an encoding protein and application thereof. The nucleotide sequence of the OsDHS gene is represented by SEQ ID NO:1 in a sequence table. The amino acid sequence of the encoding protein is represented by SEQ ID NO:2. By carrying out overexpression or knockout on the OsDHS gene in rice, a series of experiments prove that OsDHS gene overexpressed transgenic rice is super-sensitive to drought, and epicuticular wax is seriously lacked; otherwise, a dhs mutant presents that the drought resistance is enhanced. According to the OsDHS gene, by adjusting and controlling the synthesis of the epicuticular wax of the rice, the response of plants to drought stress is adjusted and controlled.

The invention discloses a method for improving drought resistance of turf on garbage compost substrate, which includes: selecting a lawn plant Festuca arundinacea and domestic garbage compost, applying 0.2-0.4Xkg-1 of rare-earth lanthanum nitrate or cerium nitrate per 60g of the compost, and mixing for use; respectively scattering 0.5g of Festuca arundinacea seeds into cups; controlling largest water-holding capacity of the compost in a field to be 66% and severe stress to be 35-50%, normally supplying water early in the experiment to guarantee smooth seed germination and seedling growth, quantitatively feeding water after 2 weeks according to the degree of severe stress, and controlling the degree of drought by weighing process; and finally measuring all indexes. Experimental results show that applying rear earth increases the ratio of chlorophyll a, chlorophyll b, content of total chlorophyll and chlorophyll a/b of Festuca arundinacea under drought stress, so that optical absorption of Festuca arundinacea is enhanced, accumulation of photosynthate is promoted, and stress resistance of the lawn plant Festuca arundinacea is improved.

The invention provides a method for efficiently selecting drought-resisting alfalfa variety. The method includes steps of (1), seedling cultivation of alfalfa; (2), simulating drought stress by nutrition liquid containing 0.38 g/mL and 0.43 g/mL PEG-6000 solution, and performing the drought stress on the alfalfa seedling root grown for 40-45 days; (3), respectively testing the following physical signs of the alfalfa under the stress of two PEG-6000 concentrations; (4), according to sensitive threshold values of every geological index under the stress of PEG-6000 solutions with different concentrations, and selecting the drought-resisting alfalfa variety. Two PEG-6000 solutions with greatly high concentrations are applied to measure the alfalfa physical signs greatly contributed to the drought resistance by simulating the strong drought stress environments; through comparing the endurance capacity of different types of drought-resisting alfalfa to high-concentration PEG stress and corresponding change threshold value of sensitive physical signs, the drought-resisting alfalfa variety can be exactly and efficiently screened.

The invention belongs to the field of plant genetic engineering technologies. The invention specifically involves the separation and cloning of a rice DNA fragment, functional verification and application. The said DNA fragment contains adversity-related gene OsPR4-1, which gives the plants the tolerant capacity against adversities such as drought threat. Combines the DNA fraction of the gene with exogenous regulating gene and introduces directly into the plant, the tolerant capacity of the plants against drought threat increases significantly.

The invention discloses a leaf tensity change-based photosynthesis predicting method and belongs to the technical fields of agricultural engineering and crop information detection. The method comprises setting different drought stress level pair models for treatment, measuring the physiological capacitance C, the leaf water potential W and the net photosynthetic rate An of leaves of a model plant, calculating the leaf tensity Td of the module plant, utilizing a rectangular hyperbolic equation to structure a mathematical model of the relationship of the leaf tensity Td and the net photosynthetic rate An of the model plant; selecting a plant to be surveyed, which grows at a draught level to be detected, measuring the physiological capacitance Cs and the leaf water potential Ws of the plant to be surveyed, and calculating the leaf tensity Tds of the plant to be surveyed; according to the structured mathematical model, determining the net photosynthetic rate Ans of the plant to be surveyed. The leaf tensity change-based photosynthesis predicting method has the advantages of being simple and rapid in operation and free from natural environment restriction and can online monitor the water status of leaves in real time and predict the change of plant photosynthesis to provide scientific basis for timely, moderate and accurate water supply.

The invention discloses a bryophyte reversal-resistant protein PpLEA3-20 and an encoding gene and an application thereof. A plant resistance-related protein provided by the invention is named PpLEA3-20, is derived from physcomitrellapatens, and is a protein shown as (1) or (2), wherein (1) is constituted by an amino acid sequence shown as a sequence 2 in a sequence table, or (2) is obtained by performing substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid residue sequence shown as the sequence 2 in the sequence table, is relevant to plant resistance and is derived from (1). As proved by an experiment for introducing the encoding gene of PpLEA3-20 into oryza sativassp.japonica, the survival rates (44 percent, 36 percent, 60 percent and 72 percent) and growth states of four obtained T2-generation PpLEA3-20 transgenic plants (20-1, 20-2, 20-3 and 20-4) in a drought stress experiment are remarkably better than those of a check plant.

The invention discloses a cutting propagation method for splash-ink dendrobe old stems. The method comprises following steps: utilizing two-year or perennial splash-ink dendrobe pseudobulb as material; utilizing a high-temperature high-humidity condition for drought stress, forming buds induced on old stems, carrying out final-period management and seedling transplant to culture new excellent parental plant. The method is utilized for obtaining cuttage seedlings with excellent parental characters. From March to May, seedlings grow. From October to December, flowers bloom. New stem sprouts germinate for 12 to 25 days. Each pseudobulb has 1 to 4 buds of more than 90% germination rate. Compared with the aseptic sowing and tissue culture propagation, the method has following beneficial effects: the method is stable in inheritance, short in culture period, fast in florescence, low in production cost, easy in operation and easy in promotion; new plants can be cultured by effective utilization of aging pseudobulb so that nutrient consumption of new pseudobulb by utilizing aging pseudobulb is reduced and service lifetime of seedlings is prolonged; and therefore, a new way is provided for splash-ink dendrobe seedling propagation and effective utilization of old stems.