268 results about "Drought stress" patented technology

The invention belongs to the technical field of drought risk prediction and vegetation influence evaluation, and particularly discloses a vegetation-to-drought resource utilization coupling response strategy evaluation method, which comprises the following steps: acquiring target data; constructing a drought index according to the target data, and extracting a drought event; extracting vegetation total productivity, vegetation net level productivity and evapotranspiration abnormal data before and after the drought event, and calculating response intensity of vegetation variables; calculating the average variation of the light energy utilization efficiency, the carbon utilization efficiency and the moisture utilization efficiency; comprehensively considering the multivariable resistance-recovery state of the vegetation physiological process and the regulating variable of the resource utilization efficiency of the vegetation in response to drought stress, and analyzing the drought event resistance-recovery response capability of the vegetation. The method can more accurately and objectively understand the physiological mechanism difference of the vegetation to the drought stress response.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention provides a tree species screening method for high-altitude drought afforestation, an afforestation method and application, and belongs to the technical field of forest tree breeding and cultivation. According to the screening method, five physiological and biochemical indexes of soluble protein, malondialdehyde, proline, peroxidase and soluble sugar in the tree species are measured through a gradient drought stress experiment, and comprehensive scoring is performed by adopting a membership function, so that the drought-resistant tree species are scientifically screened. The tree species such as sophora moorcroftiana, rosa serrulata and amorpha fruticosa screened by the method are applied to afforestation in the high-altitude arid region, and the afforestation survival rate and preservation rate can be remarkably increased. The invention provides a reliable technical scheme for solving the long-term problem that the selection of tree species in the difficult site field lacks scientific basis.

The invention relates to the technical field of gene engineering, in particular to application of a rice OsSAUR4 gene in regulation and control of rice drought tolerance, and the sequence of the rice OsSAUR4 gene is selected from any one of the following: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a protein sequence as shown in SEQ ID NO.2, and 3) a sequence of which the homology with the nucleotide sequence as shown in SEQ ID NO.1 is more than 90%. The rice OsSAUR4 gene is knocked out, so that the drought tolerance of rice is improved, and the survival rate of rice in a drought stress environment is improved.

The invention relates to the field of plant molecular biology and forest genetic engineering, and particularly provides a PagRAP2.3 protein point mutant and application thereof in drought resistance of poplar. The mutant PagRAP2.3 MA is obtained by mutating methionine at the first site and cysteine at the second site of a wild type PagRAP2.3 protein into methionine and alanine. The invention further discloses a preparation method of the mutant PagRAP2.3. An expression vector containing the PagRAP2.3 MA gene is constructed, poplar 84K is transformed through an agrobacterium-mediated method, and a transgenic line with stable expression is obtained. Functional verification results show that overexpression of PagRAP2.3 MA can significantly increase the plant height of the poplar and promote plant growth, but the sensitivity to moisture is enhanced under drought stress. The mutant can be used for regulating and controlling the growth and development of forest trees and evaluating the drought resistance, and has a forestry breeding application prospect.

The invention provides application of an OsERF8 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsERF8 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsERF8 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of an oserf8 mutant plant under PEG induction and soil dehydration drought stress is remarkably improved; and the survival rate of the overexpressed plant OsERF8-OE under the induction of PEG (Polyethylene Glycol) is obviously reduced. Compared with a wild type plant, after PEG induces drought stress, the malondialdehyde content of the overground part of the oserf8 mutant plant is reduced, and the proline content is increased; the malondialdehyde content of the overground part of the overexpressed plant OsERF8-OE is increased, and the proline content is reduced. In conclusion, the OsERF8 gene is a potential candidate new target for researching a rice drought resistance regulation mechanism.

The invention discloses application and a method of wheat demethylase TaALKBH23 in regulation and control of drought stress resistance of plants, a coding sequence of the demethylase TaALKBH23 is shown as SEQ ID NO: 1, and an amino acid sequence coded by the demethylase TaALKBH23 is shown as SEQ ID NO: 2. The invention provides demethylase TaALKBH23 and a function and application of a coding gene of the demethylase TaALKBH23 in regulation and control of plant drought resistance, and it is found through construction of a TaALKBH23 wheat genetic transformation material that the gene negatively regulates and controls wheat drought resistance. It is found that TaALKBH23 plays an important role in the process of regulating and controlling drought stress resistance of plants, and a new gene resource is provided for improving drought resistance of crops.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention relates to the technical field of biology, in particular to a tobacco NtATC3 / 4 gene and application of the tobacco NtATC3 / 4 gene to regulation and control of plant flowering and leaf surface area. The function of the NtATC3 / 4 gene is researched by using a virus induced gene silencing (VIGS) technology, and the result shows that after the NtATC3 / 4 gene is silenced, the flowering time of tobacco can be delayed, the leaf surface area can be increased and / or the stem length can be reduced. The results show that the flowering time of tobacco can be controlled by regulating and controlling the expression quantity of the NtATC3 / 4 gene, so that early flowering is prevented and controlled, the yield reduction caused by cold and drought stress is reduced, the leaf surface area is increased, and the income of tobacco growers is increased, and therefore, the NtATC3 / 4 gene has a wide market application prospect.

The invention provides a camellia oleifera seedling drought degree classification method and system based on multi-modal data, and belongs to the technical field of plant detection, the scheme is based on a ConvNeXt architecture, a graph convolutional network (GCN) and a dynamic fusion gate mechanism are combined, image data and root system data of camellia oleifera seedlings are effectively fused, fusion extraction of multi-modal data features is realized, and the method and the system have good application prospects. The classification accuracy of the drought degree of the camellia oleifera seedlings is effectively improved; according to the scheme, the spatial topology modeling capability of a leaf image is enhanced by introducing a graph convolutional network (GCN), the weights of the image and physiological features are adaptively adjusted in combination with a dynamic fusion gate, the weights of the image and root system features are adaptively adjusted through the dynamic fusion gate, the model can accurately capture complementary information among different modals, and the modeling efficiency is improved. And the accuracy of drought stress classification is remarkably improved.

The invention belongs to the technical field of biology, and particularly relates to Dendrobium officinale DoSTRK1 protein as well as a coding gene and application thereof. The invention discloses a Dendrobium officinale DoSTRK1 protein, which comprises a protein consisting of an amino acid sequence as shown in SEQ ID NO.6, and a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant drought tolerance and is derived from the protein. The invention finds that overexpression of the gene encoding the DoSTRK1 protein can enable an overexpression strain to show stronger tolerance under salt stress of a plant, and specifically, the overexpression strain shows longer roots in a seedling stage and higher chlorophyll content in a growth stage. Under drought stress, the overexpression strain also shows stronger tolerance, and compared with a wild type, the overexpression strain has the advantages that the root length in the seedling stage is increased, and the survival rate in the growth stage is higher.

The invention relates to the technical field of plant genetic engineering, in particular to a ZmbHLH166 gene related to drought resistance in the flowering stage of corn, an encoding protein and application of the ZmbHLH166 gene. The key effect of the ZmbHLH166 gene in the drought resistance of the corn in the flowering period is identified for the first time on the basis of whole genome association analysis (GWAS) and transgenic function verification, and through experiments, through genetic transformation and continuous selfing screening of a Ubi1: ZmbHLH166 overexpression vector, the obtained T2-generation corn strain of the overexpression ZmbHLH166 gene has the advantages that compared with a wild type corn strain, the yield is increased, and the yield is increased. The ZmbHLH166 gene has a shorter pollen scattering-spinning interval, which indicates that under drought stress, the overexpression of the ZmbHLH166 gene can effectively promote the synchronous development of male and female flowers of corn, thereby enhancing the drought resistance of crops. Therefore, the application of the ZmbHLH166 gene and the protein coded by the ZmbHLH166 gene in regulating and controlling the drought resistance of the corn in the flowering period is feasible, and a new and reliable gene resource and an improvement direction are provided for drought-resistant breeding of the corn.

The application belongs to the technical field of genetic engineering and relates to TaGoLS10 application of a gene and an encoded protein in regulating drought resistance of plants. TaGoLS10 The gene can positively regulate the drought resistance phenotype of wheat, and the TaGoLS10 protein encoded by the gene can specifically interact with a drought resistance related protein TaGTs, and both of them cooperatively participate in and regulate the response process of wheat to drought stress, thereby enhancing the drought resistance of wheat. The application adopts a technical strategy combining overexpression technology and RNAi technology, and successfully creates new wheat materials with significantly improved or reduced drought resistance.

The application discloses a MaDREB1F gene, and a nucleotide sequence of the MaDREB1F gene is shown as SEQ ID NO:1. Experimental research results reveal a mechanism of MaDREB1F in mediating non-biological stress resistance of bananas, and determine new targets (MaAOC4, MaACO20 and MaERF11) of MaDREB1F, which are beneficial to design breeding strategies to improve the resistance of crops to cold and drought stress.

The invention relates to the technical field of genetic engineering and molecular breeding, in particular to a ramie MYB transcription factor and application thereof. The MYB transcription factor is BnMYB6, the amino acid sequence of the MYB transcription factor is as shown in SEQ ID NO.1, and the gene sequence for coding the amino acid of the MYB transcription factor is as shown in SEQ ID NO.2. The transcription factor protein BnMYB6 can specifically and negatively regulate the biosynthesis of plant lignin, and has application value in the aspects of inhibiting the synthesis of plant lignin, promoting the growth and development of a root system and maintaining related traits such as the activity of the root system under drought stress.

This invention discloses transcription factors As NF-YB3 Its application in regulating drought resistance in oats falls under the field of biotechnology. (Transcription factors) As NF-YB3 The amino acid sequence is shown in SEQ ID NO.1. As NF-YB3 The CDS sequence of the gene is shown in SEQ ID NO.2, and the gDNA sequence is shown in SEQ ID NO.3. Overexpression As NF-YB3 The gene significantly enhances the drought resistance of oats, while also increasing the activity of CAT and SOD enzymes in oats, slowing down the water loss rate of detached leaves, and improving seedling survival rate under drought stress. AsNF-YB3 forms a heterotrimeric complex with AsNF-YA1 and AsNF-YC5, and its 4bp insertion / deletion mutation in the promoter region regulates oat drought resistance by affecting AsARF1 binding and gene expression. This provides a new gene resource and technical pathway for agricultural stress-resistance breeding.

The application belongs to the technical field of genetic engineering, and particularly discloses AtDi19 Application of the gene in drought resistance and high-oil rape breeding AtDi19 The gene is first transferred into the rape by using the transgenic technology, and the transgenic rape obtained has significantly improved drought resistance compared with the wild type rape; and the oil content of the rape seed is significantly increased under the drought stress. Therefore, the application provides a new gene resource for drought-resistant and stable high-oil rape breeding.

This disclosure relates to a compound composition comprising stearic acid and moclobemide, which can effectively improve the drought resistance of crops and promote crop growth and development under drought stress. This disclosure also provides uses of the composition and methods for improving crop growth and development under drought stress.

The application discloses a protein kinase gene positively regulating drought resistance of oats AVESA.00022b.r1.6C0001455 and application thereof, and belongs to the technical field of plant genetic engineering. AVESA.00022b.r1.6C0001455 The CDS sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2. Expression mode analysis shows that the gene is significantly induced and up-regulated under drought stress; virus-induced gene silencing verification shows that silencing the gene can significantly reduce the drought resistance of the oats; overexpression verification shows that overexpression of the gene can significantly enhance the drought resistance of the oats, and the performance is that wilting is delayed, the water loss rate is reduced, and the survival rate is improved. The application first proves that the gene is a key positive regulation factor of drought resistance in the oats, and can be used for cultivating drought-resistant transgenic plants and as a drought-resistant molecular marker for assisted breeding, and has important application value in genetic improvement of crop drought resistance.

The present invention relates to a composition, a diagnostic kit, and a diagnostic method for diagnosing drought stress in plants. Specifically, the present invention provides a composition comprising a biomarker consisting of one or more genes selected from the gene group listed in Table 1, and a kit and method capable of diagnosing the drought stress state of a plant using the same. The composition and diagnostic method according to the present invention have the effect of rapidly and accurately determining whether a plant is under drought stress by quantitatively measuring the expression level of a specific biomarker gene, thereby enabling the early identification of the growth status and appropriate countermeasures.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a Phyllostachys edulis PeMASR1 gene, biological material and application thereof. The PeMASR1 gene provided by the present application is a new gene discovered in the study of cold tolerance of Phyllostachys edulis leaves, the nucleotide sequence of which is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. Through gene cloning, expression and comparative analysis, the phylogenetic position of the Phyllostachys edulis PeMASR1 gene and the important role thereof in the research of plant response to abiotic stress mechanism and photosynthesis are revealed. Overexpression in Columbia-type Arabidopsis plants can make the rosette leaves of the plants early senesce; and the plants show a more stress-tolerant phenotype than wild-type Arabidopsis in response to cold stress, drought stress and salt stress and other abiotic stresses.

The invention discloses a kiwi fruit drought stress diagnosis model construction method, and relates to the field of kiwi fruit drought stress diagnosis, and the method comprises the steps: carrying out the terrain partitioning of a planting region, and obtaining a plurality of different differentiation planting sub-regions; determining the deployment density and the burying depth of a soil moisture sensor, and collecting time sequence data representing the soil moisture condition of each sub-region; collecting water physiological state time sequence data of the kiwi fruits, and constructing a physiological feature data set; based on an LSTM neural network model, outputting a kiwi fruit drought stress grade probability; and verifying a diagnosis result of the model by adopting a confusion matrix, calculating three evaluation indexes including accuracy, precision rate and recall rate, and dynamically updating and optimizing the model. The method has the advantages that the technical span of kiwi fruit drought stress from'uniform monitoring 'to'partition accurate diagnosis' is realized, real drought and disease stress are effectively distinguished, and reliable technical support is provided for intelligent agriculture.

The application provides application of RcSAPK2 gene in regulating drought resistance of plants and a method for regulating drought resistance of plants. In a first aspect, the application provides application of RcSAPK2 gene in regulating drought resistance of plants, wherein the RcSAPK2 gene is one of the following: 1) a nucleic acid molecule with a nucleotide sequence as shown in SEQ ID NO. 1; 2) a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO. 1 through substitution, deletion or addition of one or more nucleotides; and 3) a nucleotide sequence with at least 80% identity with SEQ ID NO. 1. Experiments show that the RcSAPK2 gene provided by the application plays an important role in resisting drought stress of plants, and provides an important basis for plant breeding.

The invention relates to the technical field of plant biology, and discloses a method for improving stress resistance of secondary metabolites of tea trees. The method comprises the following steps: collecting multi-omics time sequence data of tea trees under drought stress, and constructing a time-space aligned multi-dimensional data matrix; modeling a dynamic causal regulation relationship among genes, metabolites and phenotypes by utilizing a dynamic Bayesian network; designing a multi-dimensional evaluation algorithm fusing network topology and biological effect to screen key regulatory genes; the influence on target metabolites such as catechin is quantitatively predicted through in-vivo computer disturbance simulation; and finally generating an optimal gene editing or molecular marker assisted breeding scheme. According to the method, breakthrough from correlation analysis to causal inference is achieved, and the accuracy and efficiency of collaborative improvement of the stress resistance and the secondary metabolites of the tea trees are improved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a spartina anglica ascorbic acid peroxidase SanAPX11 gene and application, the nucleotide sequence of the SanAPX11 gene is shown as SEQ ID NO.1, and the amino acid sequence of protein coded by the SanAPX11 gene is shown as SEQ ID NO.2. After the SanAPX11 gene is transferred into a cell in the form of a plant expression vector, the SanAPX11 gene is expressed in a plant cell, so that the stress resistance of the plant, particularly drought stress resistance, oxidative stress resistance, salt stress resistance and the like, can be improved.

The invention belongs to the technical field of biology, and particularly relates to Dendrobium officinale DoORP1 protein and a coding gene and application thereof. The invention discloses a Dendrobium officinale DoORP1 protein, which comprises a protein composed of an amino acid sequence as shown in SEQ ID NO.6, and a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant drought tolerance and is derived from the Dendrobium officinale DoORP1 protein. The invention finds that overexpression of the gene coding the DoORP1 protein enables an overexpression strain to show stronger tolerance under salt stress of a plant, and the overexpression strain specifically shows that the root is longer in a seedling stage and the chlorophyll content is higher in a growth stage. Under drought stress, the overexpression strain also shows stronger tolerance, and compared with a wild type, the overexpression strain has the advantages that the root length in the seedling stage is increased, and the survival rate in the growth stage is higher.

This invention relates to the cloning and application of the stress resistance gene LpbHLH75 in *Lilium pumilum* DC. This invention aims to identify key stress resistance genes in *Lilium pumilum* through transcriptome data analysis and to study their functions. The steps include: (1) cloning of the target gene; (2) construction of a plant overexpression vector; and (3) detection of physiological indicators in transgenic plants. This experiment uses *Lilium pumilum* as the research object, cloning the LpbHLH75 gene using RT-PCR technology; transforming tobacco using an overexpression vector; identifying the function of LpbHLH75 overexpression against salt-alkali and drought stress in tobacco through transgenic tobacco, revealing the function of the *Lilium pumilum* bHLH gene under adverse stress, providing a new gene source for lily genetic engineering breeding, providing a reference for future research on lily stress resistance germplasm resources, and laying the foundation for discovering genes in *Lilium pumilum* that can enhance stress resistance and their stress resistance mechanisms.

The invention relates to a corn WRKY transcription factor ZmWRKY74 as well as a coding gene and application thereof, and belongs to the technical field of plant gene breeding, the CDS sequence of the coding gene of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.1, the gene negatively regulates the drought tolerance of crop plants, and the amino acid sequence of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.3. Gene positioning analysis is carried out on a drought-resistant mutant of corn, it is found that the ZmWRKY74 gene is an effect gene capable of improving the tolerance of corn to drought stress, and through a ZmWRKY74 gene overexpression experiment, the negative regulation effect of the ZmWRKY74 gene on the drought tolerance of corn plants is verified; important theoretical significance and application value are provided for creation of new corn germplasm and molecular breeding of corn.