719 results about "Drought stress" patented technology

The invention discloses an agricultural drought grade monitoring method based on a temperature vegetation drought index (TVDI). The method comprises the following steps of: 1, data preparation; 2, land surface temperature (LST) data reconstruction; 3, construction of crop plantation area normalized difference vegetation index-land surface temperature (NDVI-LST) feature space; 4, TVDI calculation; and 5, drought grade monitoring based on the TVDI. The invention brings forward an LST data reconstruction method based on multi-year background values and area fluctuation values, a cultivated land area multi-year NDVI-LST feature space is constructed for crops, the TVDI is calculated, a crop drought grade monitoring model based on a supervision classification idea is designed for drought grade remote sensing monitoring, the model can quite accurately reflect drought threat degrees of the crops under difference conditions in real time, and the method has great significance in monitoring, early warning and prevention of agricultural disasters.

Methods and apparatus for determining when plants require watering, and methods of attending to the watering of plants including signaling the grower that the plants are in need of hydration are provided. The novel methods include real-time measurement of plant metabolics and phytometric physiology changes of intrinsic physical or behavioral traits within the plant such as determining physiological flux measurement of enzyme flux due to environmental changes such as the wind and drought stress, soil and plant mineral deficiencies, or the interaction with a bio-control for organic disease control including, cell movement, signal transduction, internal chemical processes and external environmental processes including when plants require watering, and methods of attending to the watering of plants including signaling the grower that the plants are in need of hydration.

The invention relates to a method for improving activity of antioxidant enzyme of festuca arundinacea by using fine domestic garbage compost under drought conditions. In the method, a plastic pot with diameter of 7cm and height of 10cm is used through a pot experiment; and the method comprises the steps of: putting 150g of soil into each pot; sowing 0.5g of lawn plants in each pot; uniformly spreading 3g of municipal domestic garbage fine compost with particle sizes of 1,200nm, 600nm and 300nm on the surface of the soil; uniformly covering 20g of soil on the surface of the soil; controlling the temperature to be between 20 and 26 DEG C, wherein the relative humidity is between 40 and 60 percent, and illumination is natural light transmitting indoors; fully watering the plants in the initial stage of sowing, and watering by weighing every day to every two days according to 55 to 65 percent of water-holding capacity in fields after two weeks so as to maintain the capacity within a stress range; and measuring all the indexes on the 44th day. A result shows that the fine compost protects the festuca arundinacea plant from being damaged by active oxygen radicals, and reduces adverse environmental stresses to the plant caused by drought stresses.

The invention discloses a plant growth matrix with high water retention for spray seeding revegetation and a preparation method thereof. The plant growth matrix comprises an upper layer and a lower layer, wherein the lower layer is a plant growth matrix layer, and the upper layer is an organic matter covering layer covered on the plant growth matrix layer. According to the invention, perlite and montmorillonite with high hydroscopicity are applied to modification of the matrix for spray seeding revegetation, an organic matter is used to cover the surface of the plant growth matrix, a preparation process for the matrix is optimized, and thus, the disadvantage that moisture in a common matrix easily volatilizes is improved; the matrix for spray seeding revegetation has good adhesion, can be firmly attached on the surface of a bare rock side slope, well absorbs and maintains moisture, uninterruptedly provides moisture and nutrient elements needed by plant growth, guarantees revegetation of the side slope and has the advantages of low cost and easiness in manufacturing. Through implementation of a technical scheme in the invention, it is overcome that vegetation easily dies from drought after spray seeding of nonlocal soil on a side slope in an arid rainless region in China.

The invention discloses a directed cultivation method for a drought-resisting water-saving variety of winter wheat. The directed cultivation method mainly comprises the following steps: A, selectively pairing and hybridizing excellent complementary parent materials; and B, carrying out directed cultivation to select a new strain with strong drought resistance and high yield, wherein the step B iscarried out according to the following operations: B-1, planting early-generation F1-4 materials under high water and fertilizer conditions, and selecting based on yielding ability; and B-2, taking higher-generation materials (above generation F5) in duplicate, respectively planting under high water and fertilizer conditions and drought stress conditions in an inter-generation water-drought alternation manner, and selecting the new strain with strong drought resistance and high yield. The selected new variety of winter wheat has outstanding drought resistance and water-saving property, and has the characteristics of high yielding potential and strong stress resistance.

The invention discloses a method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi to bring the reciprocal symbiosis relation between AM fungi and host plants under drought stress into play reasonably and efficiently. According to the method, the AM fungi having a close symbiotic relation with the wheat are selectively used, and the symbiotic relation between the AM fungi and the wheat is built in a condition control mode to promote the wheat growth and improve drought adaptability. The AM fungi comprise one of glomus monosporum and acaulospora laevis, the two kinds of AM fungi are respectively propagated in an expanding mode in an expanding propagation matrix by using red clovers, and then two AM fungi inoculants and the wheat are cultured together in a symbiotic culture medium. The effective path for profitable symbiosis of the AM fungi and the host plants in adversity conditions is provided, the infection and symbiosis relation with the host plants of the AM fungi is improved in a man-made mode, and the yield of dry crops is improved.

The invention discloses a method for identifying drought resistance of corns. The method for identifying drought resistance of corns is to alternatively plant the corns in a water area and an arid area, namely a film water area (2 rows) and a film arid area (2 rows), the same material is planted in adjacent water and arid areas, and the method belongs to the technical field of corn planting. The method comprises the following steps of (1) sowing the corn seeds to be tested; (2) finally singling the corn plants to be tested; (3) conducting drought stress and normal water treatment on the corn plants to be tested; (4) conducting morphological index detection on the corns to be tested; (5) conducting yield index detection on the corns to be tested; (6) evaluating the drought resistance of the corns to be tested. A method for identifying drought resistance of corns in adult stage is established, adult corn plants are taken as detecting objects in the method, drought stress treatment is conducted on the corn from jointing stage to mature stage, through the detection of the plant morphological index and yield index, the purpose of establishing a subjective and accurate method for identifying and evaluating the drought resistance of the corns is achieved, and therefore a reliable basis for the identification of drought resistance of corns is provided.

The invention relates to a wheat CBL-CIPK (CBL-interacting protein kinase) stress tolerance regulatory factor as well as an encoding gene and an application thereof. According to the wheat CBL-CIPK stress tolerance regulatory factor, protein kinase genes which are consciously induced by salt and drought when being expressed in roots and leaves are selected according to expression profile chip information of salt and drought stress of wheat, and a cDNA (complementary deoxyribonucleic acid) sequence with a whole-length encoding area is cloned, wherein the nucleotide sequence is as shown in SEQ ID NO.1. Analysis shows that the gene can encode CBL-CIPK serine / threonine protein kinase, the amino acid sequence is as shown in SEQ ID NO.2, and is named as TaCIPK33. Transgenic function analysis shows that the gene has the functions of improving plant salt tolerance and drought resistance. Basis is provided for further understanding molecular mechanisms of wheat in responding to drought and saltstress, and meanwhile the gene can be applied to genetic improvement of novel stress tolerance germplasms of crops such as wheat.

The invention relates to a method for improving the drought resistance of drought substrate tall fescue by adopting urban household fine garbage compost, which comprises the following steps of: respectively scattering fine garbage compost of which the particle diameter is respectively 1200nm, 600nm and 300nm uniformly on the surface of soil, wherein the ratio of the particle diameter to parts by weight of the soil is 1:60-80, the temperature is controlled at 20-26 DEG C, the relative humidity is controlled at 40-60%, and natural light permeating a room is used for illuminating; fully watering at the initial stage of seeding; weighing and watering every one or two days according to 55-65% of field moisture capacity after two weeks to maintain the field moisture capacity in the stress range; and measuring each index from the 44th day. The result shows that under the drought stress, the fine compost increases the biomass of the tall fescue to a certain extent, and the research result of growth of root systems of lawn plants can be basically met by matching the compost with the substrate for cultivating turf.

The invention relates to a method for irrigating rice, which is a comprehensive technology for the water and fertilizer regulation of a rice field wetland system in rainy areas in the southern of China. Based on the agricultural irrigation and rainfall coordination idea, field water and fertilizer conversion and utilization are used as a system to be considered, rice is subjected to appropriate drought stress before irrigation under the condition that the yield of crops is not reduced, the rice is subjected to appropriate waterlogging stress when suffering from large rainfall, and the droughtstress and the waterlogging stress alternately happen, thus, the water and fertilizer contents of a rice field are scientifically adjusted, multiple arms of ensuring that the yield of regional rice is not reduced, increasing the utilization ratio of rainwater, reducing the water consumption for irrigation, reducing flood disasters, reducing water lifting energy consumption of a pump station, increasing the utilization efficiency of fertilizer and improving the regional water environment are achieved; and the comprehensive benefit of the rice field wetland system is exerted to the maximum extent.

The invention relates to a cloning and function expression method of an adversity stress AhMYBL6 gene in peanut. The adversity stress AhMYBL6 gene is synthesized by RT-PCR (reverse transcription-polymerase chain reaction) cloning by the steps of preparation and treatment of materials, extraction of RNA (ribonucleic acid) and synthesis of cDNA (complementary deoxyribonucleic acid). The open reading frame of the gene is 861bp, and 287 amino acids are coded totally. The homology of the amino acid sequence of the gene with the MYb family proteins GmMYB4, GmMYB29 and GmMYB29A2 of the soybean is higher than 60%. The fluorescent quantitative PCR verifies the expression patterns of the AhMYBL6 under low temperature, salt stress and drought stress; the result indicates that the expression levels of the AhMYBL6 are obviously enhanced after various stress treatment for 1 hour, and kept at high levels all the time thereafter; and the expression level of the AhMYBL6 in the leaf subjected to low-temperature treatment is maximally enhanced by 20 times. After the gene is transferred into peanut by transgenic means, compared with the control, the transgenic plant has obvious characteristics of cold tolerance, salt tolerance and drought tolerance. The AhMYBL6 gene can obviously enhance the stress tolerance of the peanut.

The invention provides Gingko MADS-box transcription factor gene GbMADS9 for controlling blossoming of plants; the gene is acquired by subjecting cDNA as a template to PCR (polymerase chain reaction) amplification, wherein the cDNA is acquired by reverse transcription of total RNA of gingko female flowers. The gene has the advantages that the gene GbMADS9 is capable of controlling blossoming of plants and shortening juvenile phase of plants, and under induction of GA3, ABA, salt stress, drought stress and cold damage stress, the overexpressed gene GbMADS9 is capable of enhancing plant tolerance to osmotic stress; the gene is applicable to controlling the blossoming phase of plants.

The invention discloses a compound preparation for relieving corn drought stress as well as a preparation method and application thereof, belonging to the technical field of crop stress-resistant preparation. The compound preparation disclosed by the invention is classified into a seed coating agent and a plant spray agent and contains the components such as trehalose glycolipid, betaine and calcium superphosphate; and the components are uniformly mixed and stirred according to certain proportion. After coating treatment of the seeds according to a suitable proportion, the seed germination can be effectively promoted, the seedling vigor is enhanced, the overall water regime of corn plants under drought stress is relieved, the damage degree of the plants is lightened, and the growth and development of the plants are adjusted so as to improve the corn quality and yield. By adopting the plant spray agent, a little effect of sprinkling irrigation water supply also can be realized; and the trehalose glycolipid also can realize certain inhibitory effect on the pathogenic bacteria of leaves.

The invention discloses application of a municipal domestic waste compost composite bacterial agent to improving drought resistance of lawn grass. The compost composite bacterial agent consists of liquid which is formed by diluting bacillus subtilis, actinomycetes and saccharomycetes in a volume ratio of 1 to 1 to 1 by 100 to 200 times. A result shows that drought damage of plants can be relieved to maintain a normal physiological metabolism function of the plants through activity of protective enzyme and osmotic adjustment substance content after diluted municipal domestic waste compost composite microbial bacterial agent is inoculated in the plants, so that the damage to the lawn plants caused by drought stress is effectively relieved, the drought resistance of the lawn plants is improved, and a basis is provided for planting of the lawn plants under a drought environment.

The invention relates to the technical field of plant genetic engineering, in particular to a gene for regulating and controlling the corn drought stress resistance. The gene is a ZmPP2CA10 deletion mutation gene; the deletion segment is a continuous base sequence with a distance being 301bp away from the upstream of the ZmPP2CA10 initial codon ATG; the continuous basic group sequence is shown as SEQ ID NO:1; the nucleotide sequence of the mutated ZmPP2CA10 promoter is shown as SEQ ID NO:2. The deletion mutation gene can be used for corn drought resistance relevant molecular marker auxiliary breeding and corn drought resistance auxiliary identification; the operation is convenient; the accuracy rate is high.

A method for using the promotor LEAP of rice's drought inducting gene to improve the resistance of plant to drought features that the promotor LEAP of rice OsLEAl gene is cloned. Its nucleotide sequence is shown by SEQ ID No.1 and is used to code a drought induced expression protein. Two cis-acting elements ABRE contained in its promotor region can be specifically response to drought and ABA. Its inductive expression carrier can stongly induce the expression of target gene when the drought stress is applied to a plant. The method for culturing the drought-resistant plant is also disclosed.

The invention discloses a fast screening method of drought-tolerant rice. The method can fast screen drought-tolerant rice by simulating drought stress conditions of root growth and germination of seeds by using a high-concentration high molecular polyethylene glycol penetrating fluid and determining the rooting rate and root-bud ratio of rice seeds in the highly penetrating fluid. The method is simple and rapid to operate, has good accuracy and achieves effective and fast screening of drought-tolerant rice.

The invention discloses a deep-layer infiltration method for apple orchards at dry highland areas through rainwater collected by tree disks. The deep-layer infiltration method comprises the following steps of selecting orchards; making deep-layer infiltration buckets for the collected by rainwater; trimming tree disks; burying the rainwater collection deep-layer infiltration buckets; and covering black mulching films. The deep-layer infiltration method disclosed by the invention has the beneficial effects that in the deep-layer infiltration method for the apple orchards at the dry highland areas through the rainwater collected by the tree disks, the black mulching films are covered, so that the water content of soil is effectively increased, the spring temperature and the summer temperature of the soil are effectively increased, and the microenvironment of the soil is effectively improved; the infiltration buckets of 40cm are buried, so that the water content of the soil below root layers is increased, the physical properties of the soil are changed, the growing environment of root systems is improved, water consumed for the growth of fruit trees in winter and spring drought seasons are compensated, drought stress on apple trees is small, the yield of a single plant is increased, the quality of 100 leaves of the apple trees is increased, the ratio of new shoots to short shoots is increased, the shape index of the fruit trees is increased, and the appearance of on-year and off-year of the apple trees is effectively prevented; and besides, water in rainy seasons can be effectively accumulated into deep layers, the trans-seasonal utilization of precipitation is realized, and the collected rainwater can also be used as a water soluble fertilizer for irrigation use.

The invention relates to a method for improving drought stress resistance of artificially restored Calligonum rubicundunm in a drought desert area, which comprises the following steps of nursery stock selection, early soil preparation, early nursery stock treatment, irrigation treatment and the like, and utilizes a feedback regulation system formed through drought induction of partial root systems of the Calligonum rubicundunm to improve the adaptive capacity of the Calligonum rubicundunm to the drought so as to achieve the aim of improving the integral drought stress resistance of the artificially restored Calligonum rubicundunm in the drought desert area and avoid converse succession after the artificial assistance measures are cancelled. The method realizes survival and growing development of artificially restored Calligonum rubicundunm seedlings by means of the drought stress resistance of the Calligonum rubicundunm seedlings under the natural conditions of precipitation amount less than 100 mm and drought climate after the artificial assistance measures are cancelled.

The invention relates to and aims at providing a rice RING finge family E3 ubiquitin ligase OsDHS gene as well as an encoding protein and application thereof. The nucleotide sequence of the OsDHS gene is represented by SEQ ID NO:1 in a sequence table. The amino acid sequence of the encoding protein is represented by SEQ ID NO:2. By carrying out overexpression or knockout on the OsDHS gene in rice, a series of experiments prove that OsDHS gene overexpressed transgenic rice is super-sensitive to drought, and epicuticular wax is seriously lacked; otherwise, a dhs mutant presents that the drought resistance is enhanced. According to the OsDHS gene, by adjusting and controlling the synthesis of the epicuticular wax of the rice, the response of plants to drought stress is adjusted and controlled.

The invention discloses a method for increasing the potted alpine rose number and improving the flower bud quality. The method comprises the culture medium matching, fertilizing, trimming, temperature control, drought stress treatment and illumination control. Controlled-release fertilizer can be fertilized once in the end of March; from March to the early June, ammonium sulfate can be fertilized to the root once every ten days, and full water soluble fertilizer can be fertilized to the leaf surfaces once every fifteen days; from the middle of June to the end of summer, mixed liquid fertilizer can be fertilized to the root and the leaf surfaces once every ten days; in autumn, monopotassium phosphate can be sprayed on the root and the leaf surfaces once every fifteen days; from the middle of June to the early August, the temperature is controlled within 23-28 DEG C; from the early July to the early August, the water can be controlled for 20-30 days; from the end of May to the end of August, the luminous intensity can be controlled within 33000-450001x. According to the method disclosed by the invention, the number of flower buds can be increased by 28-30% than that of conventional potted plants, the transverse diameter of the flower buds is increased by 13-18%, the problems of potted alpine rose that the flower bud forming rate is low and the flower bud quality is poor can be effectively solved, and the ornamental value of the potted alpine rose is obviously increased.

The invention discloses a method for improving drought resistance of turf on garbage compost substrate, which includes: selecting a lawn plant Festuca arundinacea and domestic garbage compost, applying 0.2-0.4Xkg-1 of rare-earth lanthanum nitrate or cerium nitrate per 60g of the compost, and mixing for use; respectively scattering 0.5g of Festuca arundinacea seeds into cups; controlling largest water-holding capacity of the compost in a field to be 66% and severe stress to be 35-50%, normally supplying water early in the experiment to guarantee smooth seed germination and seedling growth, quantitatively feeding water after 2 weeks according to the degree of severe stress, and controlling the degree of drought by weighing process; and finally measuring all indexes. Experimental results show that applying rear earth increases the ratio of chlorophyll a, chlorophyll b, content of total chlorophyll and chlorophyll a / b of Festuca arundinacea under drought stress, so that optical absorption of Festuca arundinacea is enhanced, accumulation of photosynthate is promoted, and stress resistance of the lawn plant Festuca arundinacea is improved.

The embodiment of the invention discloses a basin vegetation drought occurrence remote sensing early warning and water shortage estimation method, which comprises the following steps: step 1, spatialdata processing and information extraction: extracting multi-scale basin information, vegetation type information, runoff information, rainfall information and soil moisture information; step 2, carrying out drought stress early warning, calculating a water resource quantity variable quantity and a same-period vegetation variable quantity, and carrying out drought early warning according to the instantaneous evapotranspiration index; and step 3, estimating the drought and water shortage of the vegetation, estimating the water consumption in the specific time period of the monitoring year according to the historical average water consumption for many years in the specific time period, and estimating the water shortage in the specific time period of the monitoring year. According to the method, the problems that stress early warning cannot be carried out on existing watershed vegetation drought, and the multi-scale watershed water shortage estimation precision is not high are solved.

The invention provides a method for efficiently selecting drought-resisting alfalfa variety. The method includes steps of (1), seedling cultivation of alfalfa; (2), simulating drought stress by nutrition liquid containing 0.38 g / mL and 0.43 g / mL PEG-6000 solution, and performing the drought stress on the alfalfa seedling root grown for 40-45 days; (3), respectively testing the following physical signs of the alfalfa under the stress of two PEG-6000 concentrations; (4), according to sensitive threshold values of every geological index under the stress of PEG-6000 solutions with different concentrations, and selecting the drought-resisting alfalfa variety. Two PEG-6000 solutions with greatly high concentrations are applied to measure the alfalfa physical signs greatly contributed to the drought resistance by simulating the strong drought stress environments; through comparing the endurance capacity of different types of drought-resisting alfalfa to high-concentration PEG stress and corresponding change threshold value of sensitive physical signs, the drought-resisting alfalfa variety can be exactly and efficiently screened.

The invention belongs to the field of plant genetic engineering technologies. The invention specifically involves the separation and cloning of a rice DNA fragment, functional verification and application. The said DNA fragment contains adversity-related gene OsPR4-1, which gives the plants the tolerant capacity against adversities such as drought threat. Combines the DNA fraction of the gene with exogenous regulating gene and introduces directly into the plant, the tolerant capacity of the plants against drought threat increases significantly.

The invention relates to the technical field of wheat abiotic stress identification, in particular to a wheat drought identification method. A method for identifying the drought of wheat comprises thefollow steps of: a) carrying out a drought control experiment of wheat in a greenhouse by adopting a pot experiment method, wherein the drought grade are suitable, light drought, medium drought, severe drought and special drought; b) continuously acquiring wheat images of different drought grades by using a single-lens reflex camera, labeling the wheat images, and randomly dividing the data setsinto a training set and a test set; c) extracting image features using convolution operations of Inception_V3, Resnet_50, Resnet_152, which are pre-trained on ImageNet, and training the final classification layer; d) testing the trained depth learning network with the test set, and representing the accuracy of drought stress identification and classification with the accuracy of drought stress identification DSI and drought stress classification DSC respectively. As the wheat phenotypic characteristics are extracted by a depth learn method through acquiring a wheat digital image, the drought stress is identified and classified base on a disaster-bearing body, the drought stress is timely, accurate, lossless, and the wheat phenotypic characteristics are high in accuracy.

The invention discloses stress-resistant protein PpLEA3-17 of bryophyte as well as an encoding gene and an application thereof. The protein related to the plant stress resistance and provided by the invention is named as PpLEA3-17, is from physcomitrella patens and is the protein in 1) or 2): (1) the protein consisting of amino acid sequences shown by the sequence 2 in a sequence table; and 2) the protein which is obtained by the amino acid residue sequence of the sequence 2 in the sequence table through substitution and / or deletion and / or addition of one or more amino acid residues, is related to the plant stress resistance and is derived by 1). The experiment for introducing the encoding gene of the PpLEA3-17 disclosed by the invention into wild type rice (Nipponbare) can prove that thesurvival rate (80.4%, 68.1%, 91.8% and 69.4%) and the growing state of four T2-substituted trans-PpLEA3-17-gene plant (17-1, 17-2, 17-3 and 17-4) in the drought stress experiment are obviously betterthan those of the control plant.

The invention discloses a leaf tensity change-based photosynthesis predicting method and belongs to the technical fields of agricultural engineering and crop information detection. The method comprises setting different drought stress level pair models for treatment, measuring the physiological capacitance C, the leaf water potential W and the net photosynthetic rate An of leaves of a model plant, calculating the leaf tensity Td of the module plant, utilizing a rectangular hyperbolic equation to structure a mathematical model of the relationship of the leaf tensity Td and the net photosynthetic rate An of the model plant; selecting a plant to be surveyed, which grows at a draught level to be detected, measuring the physiological capacitance Cs and the leaf water potential Ws of the plant to be surveyed, and calculating the leaf tensity Tds of the plant to be surveyed; according to the structured mathematical model, determining the net photosynthetic rate Ans of the plant to be surveyed. The leaf tensity change-based photosynthesis predicting method has the advantages of being simple and rapid in operation and free from natural environment restriction and can online monitor the water status of leaves in real time and predict the change of plant photosynthesis to provide scientific basis for timely, moderate and accurate water supply.

The invention discloses a bryophyte reversal-resistant protein PpLEA3-20 and an encoding gene and an application thereof. A plant resistance-related protein provided by the invention is named PpLEA3-20, is derived from physcomitrellapatens, and is a protein shown as (1) or (2), wherein (1) is constituted by an amino acid sequence shown as a sequence 2 in a sequence table, or (2) is obtained by performing substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid residue sequence shown as the sequence 2 in the sequence table, is relevant to plant resistance and is derived from (1). As proved by an experiment for introducing the encoding gene of PpLEA3-20 into oryza sativassp.japonica, the survival rates (44 percent, 36 percent, 60 percent and 72 percent) and growth states of four obtained T2-generation PpLEA3-20 transgenic plants (20-1, 20-2, 20-3 and 20-4) in a drought stress experiment are remarkably better than those of a check plant.

The invention discloses a cutting propagation method for splash-ink dendrobe old stems. The method comprises following steps: utilizing two-year or perennial splash-ink dendrobe pseudobulb as material; utilizing a high-temperature high-humidity condition for drought stress, forming buds induced on old stems, carrying out final-period management and seedling transplant to culture new excellent parental plant. The method is utilized for obtaining cuttage seedlings with excellent parental characters. From March to May, seedlings grow. From October to December, flowers bloom. New stem sprouts germinate for 12 to 25 days. Each pseudobulb has 1 to 4 buds of more than 90% germination rate. Compared with the aseptic sowing and tissue culture propagation, the method has following beneficial effects: the method is stable in inheritance, short in culture period, fast in florescence, low in production cost, easy in operation and easy in promotion; new plants can be cultured by effective utilization of aging pseudobulb so that nutrient consumption of new pseudobulb by utilizing aging pseudobulb is reduced and service lifetime of seedlings is prolonged; and therefore, a new way is provided for splash-ink dendrobe seedling propagation and effective utilization of old stems.