39results about How to "Improve drought tolerance" patented technology

The invention discloses a method for reseeding and repairing alpine degraded grassland, which comprises the following steps: step 1, cutting: cutting native vegetation of a sample plot with the stubble height of 3-5cm; 2, sowing is conducted, before the rainy season comes, drilling is conducted on target grass seeds in a manual ditching mode, the target grass seeds are 2-3 grass seeds selected from each type of gramineae grass, leguminous grass and miscellaneous grass and mixed according to a certain proportion, and earthing is conducted after sowing is completed; 3, after-sowing management is conducted, agricultural measures such as impurity removal, fertilization and watering do not need to be conducted after sowing is completed, and grazing is forbidden for two years. According to the method, different functional types of plants and combinations suitable for the alpine region are screened out, the method suitable for reseeding restoration of the alpine degenerated grassland is formulated, the reseeding cost is controlled, meanwhile, plant communities high in yield, high in stability and diverse in function are built, and the problem of restoration of the degenerated grassland in the alpine region is solved.

The invention discloses application of an RLCK86 gene in improvement of drought tolerance of tomatoes, and belongs to the technical field of biology. According to the application, the tomato RLCK86 gene function is silenced or deleted by utilizing a biological technical means, so that the tolerance of tomato plants to drought stress is improved; the nucleotide sequence of a protein coding region of the RLCK86 gene is as shown in SEQ ID NO. 1. The invention discloses the application of the RLCK86 gene in regulating and controlling the drought stress resistance of the tomato for the first time, the function of the RLCK86 gene is deleted through a gene editing technology, the drought tolerance of the tomato can be remarkably improved, and a reference is provided for cultivating drought-resistant tomato germplasm resources.

The invention belongs to the field of plant genetic engineering, and particularly relates to an OsPIGL gene and a regulation and control method thereof for improving the grain length, thousand grain weight and drought tolerance of rice. The nucleotide sequence of the OsPIGL gene is as shown in SEQ ID NO: 1, and the CDS sequence is as shown in SEQ ID NO: 2; the specific application is that overexpression of the OsPIGL gene effectively improves the grain length, the thousand seed weight and the drought tolerance of the rice, and new gene resources and technical means are provided for rice breeding. The gene and the application of the gene provided by the invention are helpful for cultivating a new rice variety with excellent agronomic traits and stress resistance and improving the yield and quality of rice, and have important significance for guaranteeing grain safety.

The invention discloses a gene DobHLH14 for regulating and controlling DoSUS2 expression under drought stress to increase the content of dendrobium officinale polysaccharides and application of the gene DobHLH14, and belongs to the technical field of plant genetic engineering breeding, the nucleotide sequence of the gene DobHLH14 is shown as SEQ ID No: 4, and the coded amino acid sequence is shown as SEQ ID No: 5. The DobHLH14 is applied to the improvement of the polysaccharide content of the plant or the breeding of the drought-tolerant transgenic plant. The homologous and heterologous overexpression results of the dendrobium officinale show that the polysaccharide content and drought resistance of the transgenic plant are obviously improved by regulating the expression of the DoSUS2 gene through the DobHLH14 transcription factor of the dendrobium officinale under drought stress, and the method is an excellent method for breeding perennial herbaceous plants with high polysaccharide and drought resistance.

The invention discloses a green three-dimensional planting system for promoting agricultural economic development in an arid region. The green three-dimensional planting system comprises a climate adaptation module; the weather prediction module is responsible for providing weather change prediction; the crop selection and crop rotation module is responsible for selecting suitable drought-enduring crops and implementing scientific crop rotation; the three-dimensional planting structure module is responsible for designing a reasonable three-dimensional planting framework according to the selected drought-enduring crops; the soil improvement module is responsible for adding an organic fertilizer and a modifier and improving the soil moisture retention capability; according to the invention, through weather prediction information provided by the climate adaptation module, the system can predictively adjust an irrigation strategy, and insufficient or excessive irrigation caused by sudden weather change is avoided. The intelligent irrigation module is combined with a neural network algorithm, the irrigation amount and time are accurately calculated and automatically adjusted according to the real-time meteorological data, the drought-enduring crop characteristics, the three-dimensional planting architecture and the soil improvement condition, accurate control over irrigation is achieved, and the utilization efficiency of water resources is remarkably improved.

The invention belongs to the technical field of plant biology, and particularly relates to application of a rice OsBBX3 gene. Bioinformatics and detection analysis tests show that the protein OsBBX3 containing the B-box structural domain of the rice has the function of improving the drought tolerance of the rice, the drought tolerance of crops can be improved by improving the expression level of the OsBBX3 in the crops, the OsBBX3 gene can also be introduced into plants to construct drought-tolerant transgenic varieties, and the gene can also be used for identifying the drought-tolerant varieties.

The invention belongs to the technical field of agricultural planting, and particularly relates to a method for improving drought tolerance and yield of wheat through combined application of wormcast and sodium selenite. According to the method, the wheat is sown after the wormcast is applied to the soil, and the sodium selenite solution is sprayed when drought stress occurs, so that the growth index of the wheat under the drought stress can be remarkably improved, oxidative stress indexes such as MDA, H2O2 and O2 <-> in the wheat can be remarkably reduced, the activity of antioxidant enzymes SOD, POD, CAT and APX is increased, and meanwhile, the flour yield of wheat grains is also increased.

The invention discloses application of N-acetyl-L-cysteine in improving drought tolerance of plants, and belongs to the technical field of plant cultivation. The invention provides the application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in the improvement of the drought tolerance of the brassica napus for the first time. Experiments prove that compared with a control group, the fresh weight, the dry weight, the root length and the chlorophyll content of rape seedlings subjected to foliage spraying of 150 mu M of N-acetyl-L-cysteine under the drought stress condition are improved, the water loss rate of leaves is reduced, and the drought stress tolerance of the rape is enhanced. Therefore, the drought stress tolerance of the rape treated by the N-acetyl-L-cysteine is obviously improved. Furthermore, an optimal application concentration of 150 mu M is obtained through experimental exploration, so that the N-acetyl-L-cysteine can be optimally utilized in improving the drought stress tolerance of the oilseed rape.

The invention discloses a rose drought-enduring gene RrMYB2 and application thereof. The nucleotide sequence of the RrMYB2 gene is shown as SEQ ID NO.1, and the protein amino acid sequence of the RrMYB2 gene is shown as SEQ ID NO.2. The invention further discloses a preparation method of the rose drought-enduring gene RrMYB2. According to the application method, the drought resistance of the rosa plants is improved by controlling the overexpression of the gene RrMYB2 in the rosa plants. After the RrMYB2 gene of the purple-branch rose is separated, the RrMYB2 gene is transferred into a model plant arabidopsis thaliana and the purple-branch rose, and the drought resistance of the transgenic arabidopsis thaliana and the purple-branch rose is remarkably enhanced, so that the RrMYB2 gene is a positive regulation factor of the drought resistance and has an important application value in the field of drought-resistant and stress-resistant breeding of rose plants.

The invention discloses a sweet potato IbNFYA3 gene and application of the sweet potato IbNFYA3 gene in improvement of plant stress resistance, and belongs to the technical field of molecular biology. The amino acid sequence coded by the sweet potato IbNFYA3 gene is shown as SEQ ID NO.2, and the nucleotide sequence of the sweet potato IbNFYA3 gene is shown as SEQ ID NO.1. Constructing a plant expression vector of the IbNFYA3 gene, and transforming arabidopsis thaliana; the result shows that the morphological development of the arabidopsis thaliana plant with the transferred IbNFYA3 gene is normal, and the arabidopsis thaliana seedling with the transferred IbNFYA3 gene at least can resist the stress of 125mM NaCl or 300mM mannitol; the expression of the IbNFYA3 gene in arabidopsis thaliana can significantly improve the high salinity resistance and drought resistance of the arabidopsis thaliana. Therefore, the IbNFYA3 gene has important application value in the cultivation of drought-resistant and salt-tolerant new germplasm.

The invention relates to the technical field of plant cultivation, and particularly discloses a method for improving drought tolerance of plants, sericin protein liquid and application, and the method comprises the following steps: preparing the sericin protein liquid with the concentration of 0.1-0.5 g / L, and then spraying the sericin protein liquid on leaf surfaces of tomato plants. According to the method for improving the drought tolerance of the tomato plants, the survival rate, the biomass and the water content of the tomato plants in the drought stress environment are remarkably improved.

The application discloses application of a ZmCDPK31 gene in corn drought response and belongs to the technical field of biotechnology.The application discloses the application of the ZmCDPK31 gene in corn drought response, and the CDS sequence of the ZmCDPK31 gene is shown as SEQ ID NO.2.The application knocks out the coding gene of the protein ZmCDPK31 to positively regulate the drought resistance of plants, and has a positive effect on accelerating the improvement of corn drought-resistant varieties.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

The invention discloses application of a soybean COP9 signal complex subunit 5 protein gene GmCSN5B or a promoter thereof, and relates to the technical field of gene engineering. The drought response gene GmCSN5B for coding COP9 signal complex subunit 5 protein is successfully screened by taking a cultivated soybean drought-resistant variety Dongnong 50 as a research material through transcriptomics analysis under the stress of simulated drought (8% PEG8000 water culture). Research finds that the soybean yield and the drought resistance can be improved by knocking out the gene. On one hand, the knockout GmCSN5B gene has the capabilities of improving the soybean yield and improving the drought resistance, and on the other hand, a foundation is laid for cultivation of high-yield drought-resistant soybean new germplasm and related breeding work of the high-yield drought-resistant soybean new germplasm.

The invention provides application of a white birch BpSPL2 gene in improving the drought tolerance of white birches, and belongs to the technical field of molecular biology. The invention provides over-expression of the BpSPL2 gene participating in drought tolerance of the white birch, inhibits expression of transgenic white birch plants, and can also be used for excavating downstream regulation genes and enriching regulation pathways for plant growth stress resistance; the 35S:: BpSPL2 white birch plant obtained by overexpressing the BpSPL2 gene can be used for cultivating forest trees with excellent stress-resistant growth traits, wherein the drought tolerance of the 35S:: BpSPL2 is improved; the BpSPL2 gene provided by the invention can participate in the drought resisting process of white birch, can be used for stress growth and development research of forest plants, and provides reference resources for SBP gene family function research of the plants.

The invention discloses a drought tolerance evaluation method of a sugarcane hybrid combination, and relates to the technical field of drought tolerance evaluation, and the method comprises the steps: restraining the recording frequency of each index according to a drought coefficient, obtaining the character data of sugarcane seedlings, judging the similarity of the characters of the sugarcane seedlings in different groups through similarity analysis, constructing a difference coefficient, and calculating the drought tolerance of the sugarcane hybrid combination. If the difference coefficient exceeds the expectation, performing drought tolerance evaluation on the sugarcane seedlings by using the trained drought tolerance evaluation model, generating drought tolerance after obtaining a drought tolerance score of each sugarcane seedling, and screening out a target combination from the plurality of sugarcane hybrid combinations according to the drought tolerance; and screening out target characters in a plurality of drought tolerance states according to the difference degree, and recombining the parents of the sugarcane through a pre-trained genetic algorithm to reobtain a plurality of groups of sugarcane hybridized combinations. The target combination with a good drought-enduring effect is screened out from a plurality of sugarcane hybrid combinations, and the reliability of the evaluation result is high.

The invention discloses application of apigenin and a preparation thereof in improving drought tolerance of wheat plants. The technical problem to be solved is how to improve the drought tolerance of wheat plants. The invention specifically discloses application of apigenin or salt thereof or solvate thereof in any one of the following aspects: A1) application in improving the drought tolerance of gramineous plants; a2) application in preparation of a product for improving the drought tolerance of gramineous plants. The drought tolerance of the wheat seedlings can be improved by applying the apigenin or the salt thereof or the solvate of the apigenin and the salt to the wheat seedlings, and the method can be used for industrial production.

The invention discloses an ecological protection method for a down-dip water-containing rock slope, which relates to the technical field of ecological protection slope protection, and sequentially comprises ecological restoration of the slope surface of the slope and ecological restoration of the platform surface of the slope, ecological restoration of the slope surface sequentially comprises the steps of slope surface cleaning, drilling and vine planting, water drainage hole forming and vegetation concrete spraying of a base layer and a surface layer. The ecological restoration of the platform surface sequentially comprises the steps of cleaning the platform surface, laying an impermeable layer, building an enclosure retaining wall, planting oriental arborvitae and Japanese creeper in the enclosure retaining wall, building a soil retaining ridge, planting ridge plants, arranging a soil covering layer and planting traditional Chinese medicine plants. The greening of the slope surface can be accelerated by using the vegetation concrete and the drilling planting rattan; the slope rock mass is effectively prevented from sliding along the bedding, meanwhile, the down-dip slope is greened, and the ecological protection effect of an existing down-dip rock slope is improved.

The ecological pile device with the animal and plant habitat building function comprises ecological pile units, a conical head is integrally formed at the bottom of a pile body, a pile tip is arranged at the bottom end of the conical head, a limiting ridge is integrally formed at the top of the pile head, an ecological planting groove is formed in the middle of the top of the limiting ridge, and the ecological planting groove is formed in the middle of the top of the limiting ridge. Herbaceous plants are planted in the ecological planting grooves, every two adjacent sets of ecological pile units are connected and assembled through stabilizing pieces, pile bodies are subjected to thread treatment, the insertion convenience of the pile bodies is improved, meanwhile, a soil body and the ecological pile units which are used in practice are firmer, the planting grooves are formed in the tops of the ecological pile units, and the ecological pile units are more convenient to use. Perennial herbaceous plants can be planted, the two adjacent ecological pile units are fixed through the stabilizing pieces, the mounting stability of the ecological pile units is improved, meanwhile, the uniformity of mounting of the multiple ecological pile units can be achieved, and the adjacent ecological pile units have the supporting effect.

The invention relates to a potting planting method for striga asiatica, which comprises the following steps: pre-culturing mature striga asiatica seeds by using clear water and a hormone solution containing striga asiatica lactone with a specific concentration, and limiting the pre-culturing time, so that the germination of the striga asiatica seeds in soil and the activity improvement in an early growth stage can be effectively promoted; the seedling emergence time of striga asiatica in a soil environment is greatly shortened, and rapid seedling emergence of striga asiatica in soil culture is achieved. And based on the selection of the matrix and the host plant in the method, the drought tolerance and the disease and pest resistance of the striga asiatica seeds are optimized, the resistance of the striga asiatica seeds to the environmental pressure is improved, and the relatively high seedling quality is finally obtained.

The invention provides an RrJMJ12 gene for regulating and controlling the drought resistance of roses and application of the RrJMJ12 gene, and belongs to the technical field of plant genetic engineering, the nucleotide sequence of the RrJMJ12 gene is shown as SEQ ID NO.1; the amino acid sequence of the RrJMJ12 gene is as shown in SEQ ID NO. 2. The RrJMJ12 gene provided by the invention enriches the gene resources of rose drought-tolerant breeding, has important application value in the field of rose plant drought-tolerant stress-resistant breeding, improves the drought resistance of rose plants by interfering with the RrJMJ12 gene, and is suitable for being applied to rose plant breeding of root-related phenotypes.

The invention discloses a medicago sativa MsRCAR1 gene and application thereof in plant drought resistance, and relates to the technical field of transgenosis, amino acid of MsRCAR1 protein has at least 80% identity with a sequence shown in SEQ ID NO: 1, the expression quantity of the protein and a coding gene MsRCAR1 is increased under induction of drought stress, after the MsRCAR1 gene is transferred into a target plant, the expression quantity of the MsRCAR1 gene is increased, and the expression quantity of the MsRCAR1 gene is increased. According to the invention, the stress resistance of the target plant can be effectively improved, the plant variety can be optimized and the tolerance of the plant to drought stress can be improved on the premise of not changing the basic physiological structure and nutritional value of the plant, and a new way is provided for cultivating the variety with enhanced drought tolerance and expanding the production and planting scale of the plant.

The invention discloses a high-yield and high-efficiency cultivation structure and method for mountain cassava, and belongs to the field of planting. The cultivation method includes land preparation, variety selection, planting, bud direction selection, stem determination and harvesting. The method takes seed stem selection, increasing the number of plants, determining the planting bud direction and saving planting costs as the entry points, and gives full play to the characteristics that the morphological upper end of the plant is easy to grow buds, while the morphological lower end is easy to grow roots; the seed stem length is appropriately longer than that of traditional planting, and the nutrients and water stored in the seed stem can safely survive the short drought before the rainy season, overcome the problem of drought in the key growth period of mountain cassava, enhance the drought resistance of cassava seed stems, increase the yield per unit area of ​​cassava, and is easy to harvest, with significant effects of increasing production and income. The method is simple to operate, low in cost, quick to take effect, considerable benefits, and suitable for promotion. Planting cassava in mountainous areas achieves the effect of taking measures according to local conditions, providing classified guidance, and improving the comprehensive prevention and control of soil erosion.

The invention discloses an agriophyllum squarrosum AsWUS gene, a recombinant vector and application of the agriophyllum squarrosum AsWUS gene to improvement of plant stress resistance, and relates to the technical field of plant genetic engineering. The AsWUS gene is cloned from a high-stress-resistance wild plant agriophyllum squarrosum for the first time, and experiments prove that after the AsWUS gene is over-expressed in a model plant arabidopsis thaliana, the drought tolerance and salt tolerance of the plant can be remarkably improved. Precious gene resources and effective technical means are provided for molecular breeding of crops. The AsWUS gene, the recombinant vector and the recombinant bacteria provided by the invention have good application prospects in improving drought resistance and / or salt resistance of plants.

The invention discloses a tobacco NtMYB61 gene as well as a protein and application thereof. The CDS sequence of the NtMYB61 gene capable of influencing the drought stress resistance of the tobacco is shown as SEQ ID No.2, and the genome sequence of the NtMYB61 gene is shown as SEQ ID No.1. According to the invention, the NtMYB61 gene is constructed on a plant expression vector and is transferred into tobacco through a traditional gene overexpression and transgenic technology, so that the tobacco is overexpressed, and an overexpressed material plant shows significantly reduced drought resistance compared with a wild type plant; the NtMYB61 gene in the tobacco genome is knocked out through a gene editing technology, and the obtained NtMYB61 mutant material shows significantly enhanced drought stress resistance compared with a wild type control.

The invention provides the application of the StSDD1 gene in enhancing the drought tolerance of the potatoes by regulating and controlling the stomatal development of the potatoes, reducing the stomatal density and enhancing the oxidation resistance of the potatoes. The stomatal density and drought resistance of a transgenic plant are analyzed, the molecular mechanism that the StSDD1 gene regulates the potato to respond to drought stress by regulating the stomatal density is analyzed, and a candidate gene is provided for purposefully utilizing the gene to carry out potato molecular breeding.

The present invention discloses the use of a protein or a substance for regulating the expression of a gene encoding the protein or a substance for regulating the activity or content of the protein in regulating plant stress resistance. The protein is MsSPL4 protein, and the MsSPL4 protein is any one of the following proteins: A1) a protein whose amino acid sequence is that shown in SEQ ID No. 2; A2) a protein obtained by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence shown in A1) and having more than 80% identity with the protein shown in A1) and having the function of regulating plant stress resistance; A3) a fusion protein obtained by connecting a protein tag to the N-terminus or / and C-terminus of A1) or A2).

The invention discloses a cotton GhCAR4 gene and application thereof in drought resistance, and belongs to the field of gene engineering. The coding amino acid sequence of the GhCAR4 gene is as shown in SEQ ID NO. 1, and the nucleotide sequence of the GhCAR4 gene is as shown in SEQ ID NO. 2. The invention finds that the GhCAR4 responds to drought induction, compared with a wild plant, overexpression of the GhCAR4 can enhance the growth vigor, drought tolerance and antioxidant enzyme activity of arabidopsis thaliana seedlings, and virus-mediated GhCAR4 silencing reduces the drought tolerance and T-AOC activity of cotton, which indicates that the GhCAR4 positively participates in drought response and is an excellent candidate gene for drought resistance genetic improvement of cotton.

The invention discloses application of an OsCPK12 gene in regulation and control of photosynthesis of rice, and belongs to the technical field of gene engineering. The login number of the OsCPK12 gene is LOCOs04g47300, and the nucleotide sequence of the OsCPK12 gene is as shown in SEQ ID NO. 5. Photo-induction OsCPK12 mediated OsAtpD1 phosphorylation promotes introduction of OsAtpD1 into chloroplast to participate in ATP synthase, directly affects ATP enzyme activity, and indirectly affects expression of chlorophyll synthesis related genes and related proteins on a photosynthetic electron transport chain so as to participate in a dynamic regulation process of photosynthesis. The invention can provide candidate genes and theoretical basis for high photosynthetic efficiency breeding of rice.

The present application relates to the field of biotechnology, in particular to a strain CXL-18 of a layered near-melanosporum and application thereof.The strain CXL-18 of the present application can effectively promote the growth of pear trees, and also improve the drought resistance of pear trees; after the pear trees are inoculated with the strain, the plant height, stem diameter, leaf area and dry weight are all significantly higher than those of the control group without inoculation; from the appearance, the growth of the plants is obviously better than that of the control group; under the stress of moderate drought conditions, the leaf area is significantly increased by 33.50% compared with the control, and the relative water content of the leaf is significantly increased by 27.06% compared with the control; from the growth of the plants, the growth is also better than that of the control group, which shows that the strain CXL-18 has good growth-promoting ability for pear trees, can improve the drought resistance of pear trees, and can provide a new idea for the research and development of pear tree biological bacterial fertilizer.