19results about How to "Improve drought tolerance" patented technology

The invention discloses application of an RLCK86 gene in improvement of drought tolerance of tomatoes, and belongs to the technical field of biology. According to the application, the tomato RLCK86 gene function is silenced or deleted by utilizing a biological technical means, so that the tolerance of tomato plants to drought stress is improved; the nucleotide sequence of a protein coding region of the RLCK86 gene is as shown in SEQ ID NO. 1. The invention discloses the application of the RLCK86 gene in regulating and controlling the drought stress resistance of the tomato for the first time, the function of the RLCK86 gene is deleted through a gene editing technology, the drought tolerance of the tomato can be remarkably improved, and a reference is provided for cultivating drought-resistant tomato germplasm resources.

The invention discloses a gene DobHLH14 for regulating and controlling DoSUS2 expression under drought stress to increase the content of dendrobium officinale polysaccharides and application of the gene DobHLH14, and belongs to the technical field of plant genetic engineering breeding, the nucleotide sequence of the gene DobHLH14 is shown as SEQ ID No: 4, and the coded amino acid sequence is shown as SEQ ID No: 5. The DobHLH14 is applied to the improvement of the polysaccharide content of the plant or the breeding of the drought-tolerant transgenic plant. The homologous and heterologous overexpression results of the dendrobium officinale show that the polysaccharide content and drought resistance of the transgenic plant are obviously improved by regulating the expression of the DoSUS2 gene through the DobHLH14 transcription factor of the dendrobium officinale under drought stress, and the method is an excellent method for breeding perennial herbaceous plants with high polysaccharide and drought resistance.

The invention discloses a green three-dimensional planting system for promoting agricultural economic development in an arid region. The green three-dimensional planting system comprises a climate adaptation module; the weather prediction module is responsible for providing weather change prediction; the crop selection and crop rotation module is responsible for selecting suitable drought-enduring crops and implementing scientific crop rotation; the three-dimensional planting structure module is responsible for designing a reasonable three-dimensional planting framework according to the selected drought-enduring crops; the soil improvement module is responsible for adding an organic fertilizer and a modifier and improving the soil moisture retention capability; according to the invention, through weather prediction information provided by the climate adaptation module, the system can predictively adjust an irrigation strategy, and insufficient or excessive irrigation caused by sudden weather change is avoided. The intelligent irrigation module is combined with a neural network algorithm, the irrigation amount and time are accurately calculated and automatically adjusted according to the real-time meteorological data, the drought-enduring crop characteristics, the three-dimensional planting architecture and the soil improvement condition, accurate control over irrigation is achieved, and the utilization efficiency of water resources is remarkably improved.

The invention discloses application of N-acetyl-L-cysteine in improving drought tolerance of plants, and belongs to the technical field of plant cultivation. The invention provides the application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in the improvement of the drought tolerance of the brassica napus for the first time. Experiments prove that compared with a control group, the fresh weight, the dry weight, the root length and the chlorophyll content of rape seedlings subjected to foliage spraying of 150 mu M of N-acetyl-L-cysteine under the drought stress condition are improved, the water loss rate of leaves is reduced, and the drought stress tolerance of the rape is enhanced. Therefore, the drought stress tolerance of the rape treated by the N-acetyl-L-cysteine is obviously improved. Furthermore, an optimal application concentration of 150 mu M is obtained through experimental exploration, so that the N-acetyl-L-cysteine can be optimally utilized in improving the drought stress tolerance of the oilseed rape.

The invention discloses a rose drought-enduring gene RrMYB2 and application thereof. The nucleotide sequence of the RrMYB2 gene is shown as SEQ ID NO.1, and the protein amino acid sequence of the RrMYB2 gene is shown as SEQ ID NO.2. The invention further discloses a preparation method of the rose drought-enduring gene RrMYB2. According to the application method, the drought resistance of the rosa plants is improved by controlling the overexpression of the gene RrMYB2 in the rosa plants. After the RrMYB2 gene of the purple-branch rose is separated, the RrMYB2 gene is transferred into a model plant arabidopsis thaliana and the purple-branch rose, and the drought resistance of the transgenic arabidopsis thaliana and the purple-branch rose is remarkably enhanced, so that the RrMYB2 gene is a positive regulation factor of the drought resistance and has an important application value in the field of drought-resistant and stress-resistant breeding of rose plants.

The application discloses application of a ZmCDPK31 gene in corn drought response and belongs to the technical field of biotechnology.The application discloses the application of the ZmCDPK31 gene in corn drought response, and the CDS sequence of the ZmCDPK31 gene is shown as SEQ ID NO.2.The application knocks out the coding gene of the protein ZmCDPK31 to positively regulate the drought resistance of plants, and has a positive effect on accelerating the improvement of corn drought-resistant varieties.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

The invention discloses an ecological protection method for a down-dip water-containing rock slope, which relates to the technical field of ecological protection slope protection, and sequentially comprises ecological restoration of the slope surface of the slope and ecological restoration of the platform surface of the slope, ecological restoration of the slope surface sequentially comprises the steps of slope surface cleaning, drilling and vine planting, water drainage hole forming and vegetation concrete spraying of a base layer and a surface layer. The ecological restoration of the platform surface sequentially comprises the steps of cleaning the platform surface, laying an impermeable layer, building an enclosure retaining wall, planting oriental arborvitae and Japanese creeper in the enclosure retaining wall, building a soil retaining ridge, planting ridge plants, arranging a soil covering layer and planting traditional Chinese medicine plants. The greening of the slope surface can be accelerated by using the vegetation concrete and the drilling planting rattan; the slope rock mass is effectively prevented from sliding along the bedding, meanwhile, the down-dip slope is greened, and the ecological protection effect of an existing down-dip rock slope is improved.

The invention relates to a potting planting method for striga asiatica, which comprises the following steps: pre-culturing mature striga asiatica seeds by using clear water and a hormone solution containing striga asiatica lactone with a specific concentration, and limiting the pre-culturing time, so that the germination of the striga asiatica seeds in soil and the activity improvement in an early growth stage can be effectively promoted; the seedling emergence time of striga asiatica in a soil environment is greatly shortened, and rapid seedling emergence of striga asiatica in soil culture is achieved. And based on the selection of the matrix and the host plant in the method, the drought tolerance and the disease and pest resistance of the striga asiatica seeds are optimized, the resistance of the striga asiatica seeds to the environmental pressure is improved, and the relatively high seedling quality is finally obtained.

The invention provides an RrJMJ12 gene for regulating and controlling the drought resistance of roses and application of the RrJMJ12 gene, and belongs to the technical field of plant genetic engineering, the nucleotide sequence of the RrJMJ12 gene is shown as SEQ ID NO.1; the amino acid sequence of the RrJMJ12 gene is as shown in SEQ ID NO. 2. The RrJMJ12 gene provided by the invention enriches the gene resources of rose drought-tolerant breeding, has important application value in the field of rose plant drought-tolerant stress-resistant breeding, improves the drought resistance of rose plants by interfering with the RrJMJ12 gene, and is suitable for being applied to rose plant breeding of root-related phenotypes.

The invention discloses an agriophyllum squarrosum AsWUS gene, a recombinant vector and application of the agriophyllum squarrosum AsWUS gene to improvement of plant stress resistance, and relates to the technical field of plant genetic engineering. The AsWUS gene is cloned from a high-stress-resistance wild plant agriophyllum squarrosum for the first time, and experiments prove that after the AsWUS gene is over-expressed in a model plant arabidopsis thaliana, the drought tolerance and salt tolerance of the plant can be remarkably improved. Precious gene resources and effective technical means are provided for molecular breeding of crops. The AsWUS gene, the recombinant vector and the recombinant bacteria provided by the invention have good application prospects in improving drought resistance and / or salt resistance of plants.

The invention discloses a tobacco NtMYB61 gene as well as a protein and application thereof. The CDS sequence of the NtMYB61 gene capable of influencing the drought stress resistance of the tobacco is shown as SEQ ID No.2, and the genome sequence of the NtMYB61 gene is shown as SEQ ID No.1. According to the invention, the NtMYB61 gene is constructed on a plant expression vector and is transferred into tobacco through a traditional gene overexpression and transgenic technology, so that the tobacco is overexpressed, and an overexpressed material plant shows significantly reduced drought resistance compared with a wild type plant; the NtMYB61 gene in the tobacco genome is knocked out through a gene editing technology, and the obtained NtMYB61 mutant material shows significantly enhanced drought stress resistance compared with a wild type control.

The present invention discloses the use of a protein or a substance for regulating the expression of a gene encoding the protein or a substance for regulating the activity or content of the protein in regulating plant stress resistance. The protein is MsSPL4 protein, and the MsSPL4 protein is any one of the following proteins: A1) a protein whose amino acid sequence is that shown in SEQ ID No. 2; A2) a protein obtained by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence shown in A1) and having more than 80% identity with the protein shown in A1) and having the function of regulating plant stress resistance; A3) a fusion protein obtained by connecting a protein tag to the N-terminus or / and C-terminus of A1) or A2).

The invention discloses a cotton GhCAR4 gene and application thereof in drought resistance, and belongs to the field of gene engineering. The coding amino acid sequence of the GhCAR4 gene is as shown in SEQ ID NO. 1, and the nucleotide sequence of the GhCAR4 gene is as shown in SEQ ID NO. 2. The invention finds that the GhCAR4 responds to drought induction, compared with a wild plant, overexpression of the GhCAR4 can enhance the growth vigor, drought tolerance and antioxidant enzyme activity of arabidopsis thaliana seedlings, and virus-mediated GhCAR4 silencing reduces the drought tolerance and T-AOC activity of cotton, which indicates that the GhCAR4 positively participates in drought response and is an excellent candidate gene for drought resistance genetic improvement of cotton.

The present application relates to the field of biotechnology, in particular to a strain CXL-18 of a layered near-melanosporum and application thereof.The strain CXL-18 of the present application can effectively promote the growth of pear trees, and also improve the drought resistance of pear trees; after the pear trees are inoculated with the strain, the plant height, stem diameter, leaf area and dry weight are all significantly higher than those of the control group without inoculation; from the appearance, the growth of the plants is obviously better than that of the control group; under the stress of moderate drought conditions, the leaf area is significantly increased by 33.50% compared with the control, and the relative water content of the leaf is significantly increased by 27.06% compared with the control; from the growth of the plants, the growth is also better than that of the control group, which shows that the strain CXL-18 has good growth-promoting ability for pear trees, can improve the drought resistance of pear trees, and can provide a new idea for the research and development of pear tree biological bacterial fertilizer.

The application discloses a planting method for relieving drought of wheat based on combined application of SiQDs seed dressing and halophilic bacillus, and belongs to the technical field of agricultural planting. The application discloses a planting method for relieving drought of wheat based on combined application of SiQDs seed dressing and halophilic bacillus, which comprises the following steps: using silicon quantum dots (SiQDs) for seed dressing treatment of wheat seeds, then planting the seed-dressed wheat seeds in soil, and applying halophilic bacillus agent during the growth of the wheat. The combined application of SiQDs and halophilic bacillus GS127 can better promote the root development of wheat under drought stress, increase the content of proline and soluble sugar in the wheat, reduce the content of malondialdehyde, enhance the drought resistance of the wheat, effectively promote the growth of the wheat, and improve the yield and water use efficiency, and therefore has excellent application potential in the production in agricultural wheat drought areas.

The invention discloses a celery photosynthetic carbon assimilation key enzyme gene AgRbcS3 and its application under drought stress, and relates to the field of biotechnology. The nucleotide sequence of the celery photosynthetic carbon assimilation key enzyme gene AgRbcS3 is shown in SEQ ID NO.1. The invention clones a new RbcS gene, AgRbcS3, from the celery variety 'Jinyun Baoqin', and describes its function in plant drought resistance. The invention first verifies that AgRbcS3 transgenic yeast has stronger drought resistance through yeast expression, and then transfers the AgRbcS3 gene into Arabidopsis thaliana and 'Liuhe Huangxinqin' celery variety callus tissue by Agrobacterium tumefaciens-mediated method. As a result, it is found that the drought resistance of the obtained transgenic plants and transgenic callus is significantly improved, which proves that the AgRbcS3 gene provided by the invention can improve the tolerance of plants to drought stress and has broad application prospects.

The present invention discloses the application of CircCDPK6 gene in regulating plant drought tolerance, belonging to the field of biotechnology. The sequence of the CircCDPK6 gene is shown in SEQ ID NO.1. The present invention constructs CircCDPK6RNAi transgenic corn strains and CircCDPK6OE transgenic Arabidopsis plants, conducts functional studies on the CircCDPK6 gene, and reveals that when the expression of CircCDPK6 is inhibited, the drought tolerance of corn is weakened; increasing the expression of CircCDPK6 enhances the drought tolerance of Arabidopsis plants. The present invention provides gene resources for drought-resistant breeding and genetic improvement of corn and other plants.

The application belongs to the technical field of genetic engineering, and discloses TaSIRH2-14 A gene and application thereof in plant drought resistance. TaSIRH2-14 The nucleotide sequence of the gene is shown as SEQ ID NO:1, the gene positively regulates drought resistance of plants, overexpression TaSIRH2-14 The gene improves drought resistance of plants. After overexpression TaSIRH2-14 of the gene, the survival rate of plants under water deficiency conditions is improved, the gene significantly improves the tolerance of plants to drought stress through the pathways of improving water retention capacity of plants, reducing membrane lipid peroxidation damage, promoting proline accumulation, enhancing antioxidant enzyme activity and the like. The application provides a germplasm resource for wheat drought resistance breeding, and provides a new idea for cultivating drought-resistant wheat varieties through molecular breeding.