376 results about "Drought tolerance" patented technology

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to improve the corn plant's drought tolerance and ultimate production yield in hot weather. By selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then infused to the soil in which the corn seeds and / or seedlings are planted. Optionally, the KHP solution can be diluted by water, as disclosed in the specification, for applying to the soil.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to enhance the drought tolerance attribute of wheat plants. By selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then infused to the soil in which the wheat seeds are planted; the solution can also be sprayed to the leaf surface of young wheat plants. Optionally, the KHP solution can be diluted by water, as disclosed in the specification, for applying to the soil around the wheat plants and for spraying to leaf surface of young wheat plants.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The invention provides a method for screening drought-enduring germplasm resources of macadamia nuts, and belongs to the technical field of plant germplasm resource screening. The screening method comprises the following steps: (1) sowing disinfected macadimia nut seeds into a seedling raising tray, and raising seedlings to obtain seedlings; (2) transplanting the seedlings obtained in the step (1) into a nutrient medium, and performing drought stress culture to obtain pretreated seedlings; and (3) determining drought tolerance indexes in the pretreated seedlings obtained in the step (2), and screening to obtain the macadimia nut drought-tolerant germplasm resources. According to the screening method provided by the invention, macadamia nut germplasm resources with relatively strong drought tolerance can be rapidly screened out, and powerful support is provided for cultivation of drought-tolerant varieties.

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention discloses application of an OsCPN10a gene in regulation and control of vigor, grain shape and drought tolerance of rice seeds, and belongs to the technical field of rice gene engineering. The invention particularly relates to the research of the OsCPN10a gene in the aspects of regulating and controlling the germination rate, the germination potential, the germination index, the grain length, the grain width, the thousand grain weight, the drought tolerance of the rice seeds and the like. Experimental results show that the grain shape of the OsCPN10a knockout mutant seed is obviously lengthened and widened, and the grain shape of the OsCPN10a overexpression seed is opposite; the yield of the Ocpn10a mutant is higher than that of a TNG67 plant; overexpression of the OsCPN10a has the potential of improving the drought resistance of the rice. The invention reveals that OsCPN10a affects genetic mechanisms such as rice seed vigor, grain length, grain width and storability, provides important gene resources and theoretical basis for genetic improvement of rice, and is beneficial to cultivation of a new rice variety with better grain shape and higher yield.

The invention discloses a bacillus thuringiensis L3 and an application thereof. The preservation number of the bacillus thuringiensis L3 is CCTCC (China Center for Type Culture Collection) NO: M 2025487. The gene sequence of the 16S rDNA of the bacillus thuringiensis L3 is as shown in SEQ ID NO: 1. Under different adversity stress conditions, the bacillus thuringiensis L3 shows a certain stress resistance potential. Research finds that the bacillus thuringiensis L3 has certain acid resistance, high temperature resistance, cholate resistance and drought resistance, and has strong alkali resistance.

The present disclosure provides genetically modified plants, plant cells, or plant tissues wherein the plants, plant cells, or plant tissues comprise an exogenous nucleic acid comprising a nucleotide sequence encoding a 30S ribosomal protein or a homolog thereof, wherein the 30S ribosomal protein or the homolog thereof is expressed in the plant, plant cell, or plant tissue. The current disclosure provides methods of improving drought tolerance and water loss in a plant, plant cell, or plant tissue, the methods comprising an exogenous nucleic acid sequence encoding a 30S ribosomal protein or a homolog thereof, wherein the 30S ribosomal protein or the homolog thereof is expressed in the plant, plant cell, or plant tissue. Additionally, the current disclosure is describes to genetically modified plants, plant cells, or plant tissues wherein the plants, plant cells, or plant tissues comprise an exogenous nucleic acid comprising a nucleotide sequence encoding a cation / H+ exchanger 20 (CHX20) protein or a homolog thereof, wherein the nucleotide sequence is operably linked to a heterologous promoter, and the CHX20 protein or homolog thereof is expressed in the plant, plant cell, or plant tissue. Finally, the current disclosure provides methods of improving drought tolerance and water loss in a plant, plant cell, or plant tissue, the methods comprising an exogenous nucleic acid sequence encoding a CHX20 or a homolog thereof, wherein the CHX20 or the homolog thereof is expressed in the plant, plant cell, or plant tissue.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying drought tolerance of wheat, haplotypes, primers and application. Belongs to the technical field of biological breeding. Research finds that three SNP loci exist on a wheat ARF20-B gene, a wheat drought-enduring haplotype ARF20-BHapI is developed based on the SNP loci, the first locus is located at the 3616bp position of the ARF20-B gene, and the A / G nucleotide polymorphism is A; the second site is located at the 5623bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism; the third site is located at the 9795bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism. The wheat carrying the ARF20-BHapI haplotype has the advantage that the drought tolerance of the wheat carrying the ARF20-BHapI haplotype is higher. The invention further provides a KASP primer group for detecting the ARF20-BHapI haplotype, and successful detection of the drought tolerance of the wheat is achieved.

The invention provides a wheat drought-tolerant gene TaTZF1 and application thereof in plant drought tolerance, and belongs to the technical field of gene engineering. The invention provides a wheat drought-enduring gene TaTZF1. The amino acid sequence of a protein encoded by the wheat drought-enduring gene TaTZF1 is as shown in SEQ ID No. 2. The wheat drought-enduring gene TaTZF1 is subjected to induced expression by drought, ABA and salt stress, the wheat drought-enduring gene TaTZF1 is overexpressed and transformed into wheat in an overexpression mode, and it is found that the drought tolerance of a transgenic plant with overexpressed TaTZF1 is remarkably improved compared with that of a wild type plant; after the wheat drought-tolerant gene TaTZF1 in wheat is knocked out, the drought tolerance is obviously reduced. It is proved that the wheat drought-resistant gene TaTZF1 positively regulates the drought resistance of wheat, gene resources are provided for wheat molecular breeding, and the wheat drought-resistant gene TaTZF1 has great significance in improvement of wheat drought-resistant genetic improvement.

The invention discloses application of a corn ZmTST1 gene in improvement of plant yield and stress resistance. The nucleotide sequence of the corn ZmTST1 gene is as shown in SEQ ID NO. 1; the stress resistance is tolerance to salt stress and drought stress. Through sequence analysis and functional verification, after the ZmTST1 gene is over-expressed, the growth of plants can be promoted, the yield of the plants can be increased, meanwhile, the drought tolerance and the salt tolerance of ZmTST1 expressed plants are obviously higher than those of wild plants, and it is proved that the ZmTST1 gene has a remarkable effect in the aspects of high yield and stress resistance. By identifying the ZmTST1 gene, plants with high stress resistance can be screened, the cultivation period of the plants can be shortened, and a new method and thought are provided for plant breeding in the future.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsSRO1c gene in controlling high-temperature resistance of rice. The OsSRO1c gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsSRO1c gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The gene OsSRO1c for controlling rice high-temperature response is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, high-temperature stress phenotype identification in a seedling stage and an adult-plant stage shows that when a gene segment is deleted, the high-temperature resistance of rice is reduced, and when the expression quantity of the gene is increased, the high-temperature resistance of rice is improved, and the function and the application way of the gene are proved.

The invention relates to a deep learning-based plant drought tolerance prediction method and system, and the method comprises the steps: obtaining SPAD values and environment variable data corresponding to each sampling time point of a to-be-detected plant in a set time period, carrying out the preprocessing, obtaining predicted SPAD values through a trained prediction model, predicting the change trend between the SPAD values according to the SPAD values collected by the to-be-detected plant, and carrying out the prediction of the drought tolerance of the to-be-detected plant. Determining the drought tolerance of the to-be-detected plant; the prediction model obtains local features and global features in time sequence data through a time convolutional network layer, and the obtained features extract a long-term dependency relationship in a feature sequence by using bidirectional LSTM; performing time step weighting processing on the input features through a first user-defined attention mechanism module; and through a second self-defined attention mechanism module, multi-head attention weighting is performed on the feature vector after time weighting, attention output is performed by splicing a weighting result of each attention head, and a predicted SPAD value is obtained through post-processing.

The invention discloses application of GmFLZ protein or a coding gene thereof in drought tolerance of plants, in particular to application in drought tolerance of soybeans. The soybean GmFLZ protein or the coding gene thereof provided by the invention has a soybean drought-enduring function. According to the invention, the GmFLZ gene is introduced into soybean roots through an agrobacterium transformation method, so that a GmFLZ gene overexpressed soybean complex plant is obtained, and the drought tolerance of soybeans is remarkably improved.

The invention relates to the technical field of gene engineering, in particular to application of a rice OsSAUR4 gene in regulation and control of rice drought tolerance, and the sequence of the rice OsSAUR4 gene is selected from any one of the following: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a protein sequence as shown in SEQ ID NO.2, and 3) a sequence of which the homology with the nucleotide sequence as shown in SEQ ID NO.1 is more than 90%. The rice OsSAUR4 gene is knocked out, so that the drought tolerance of rice is improved, and the survival rate of rice in a drought stress environment is improved.

The invention discloses a method for improving drought tolerance and yield of wheat through combined application of compost and sodium selenite, and belongs to the technical field of agricultural planting. According to the method, wheat is planted after compost is applied to soil, and when drought stress occurs, a sodium selenite solution is sprayed to wheat leaves, so that oxidative stress indexes such as MDA, H2O2 and O2 <-> of wheat can be remarkably reduced, and the activity of antioxidant enzymes such as SOD, POD, CAT and APX can be improved; physiological indexes such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, protein, soluble sugar and proline of wheat leaves are improved, growth and yield related traits of the wheat are improved, and finally the drought resistance and yield of the wheat are improved. The compost is prepared by taking 50%-55% of corn straw, 10%-20% of flue-cured tobacco straw, 20%-25% of rice straw, 10%-15% of livestock manure and 0.1%-1% of bacillus subtilis as raw materials, adding organic wastewater or tap water, mixing, composting and fermenting.

Disclosed are a Solanum tuberosum drought tolerance-related transcription factor gene StPIF4 and the use thereof. A CDS sequence of StPIF4 has a length of 1554 bp, has a nucleotide sequence shown as SEQ ID No. 1, and encodes 517 amino acids, the amino acid sequence being shown as SEQ ID No. 2. The gene is located in a cell nucleus. The gene reduces the water loss of Solanum tuberosum seedlings under the drought stress by adjusting the opening degree of stomas, thus enhancing the drought tolerance of Solanum tuberosum. The present invention provides a new genetic material and a theoretical basis for analyzing the drought-tolerant molecular mechanism and drought-tolerant breeding improvement of Solanum tuberosum.

The invention discloses an application of a salvia miltiorrhiza SmNCED2 gene and an encoding protein thereof in drought resistance. The invention specifically discloses a protein with amino acid shown as SEQ ID No.1, a coding gene, a related biological material and application of the protein in regulation and control of drought resistance of salvia miltiorrhiza. The SmNCED2 gene from salvia miltiorrhiza is introduced into receptor salvia miltiorrhiza to obtain a transgenic salvia miltiorrhiza plant over-expressing the SmNCED2 gene, and drought tolerance agronomic trait identification and ABA index determination results show that compared with control salvia miltiorrhiza, the salvia miltiorrhiza plant over-expressing the SmNCED2 gene can significantly improve the drought resistance of salvia miltiorrhiza under the drought stress condition, so that the drought resistance of salvia miltiorrhiza is improved, and the drought resistance of salvia miltiorrhiza is improved. The SmNCED2 gene and the encoding protein thereof provided by the invention have important application value in regulating the drought resistance of salvia miltiorrhiza.

The invention provides an application of a sunflower HaSnRK2 gene in regulating and controlling salt tolerance and drought tolerance of plants, and belongs to the technical field of plant genetic engineering. The invention provides an overexpression vector of a sunflower HaSnRK2 gene. The overexpression vector is applied to regulation and control of salt tolerance and drought tolerance of plants after genetic transformation is carried out on the plants by using a flower dipping method. Through overexpression of the sunflower HaSnRK2 gene, the salt stress tolerance and the drought stress tolerance of plants are remarkably enhanced, and damage caused by reactive oxygen free radicals induced by salt stress and drought stress can be effectively relieved.

A cultivation box for improving drought resistance of peanut seeds comprises a cultivation frame, a plurality of same cultivation chambers are arranged on the cultivation frame, cultivation boxes are detachably connected into the cultivation chambers, a bottom containing box is arranged at the bottom of the cultivation frame, and an electric control box, a soil preparation box and a water tank are arranged in the bottom containing box. According to the cultivation box capable of improving the drought resistance of the peanut seeds, the multiple independent cultivation boxes are arranged in an integrated mode, multiple sets of cultivation experiments can be conducted at a time, the equipment cost is low, the occupied area is small, the cultivation boxes and the cultivation chambers are independently arranged and do not interfere with each other, and the cultivation box is convenient to use. The growth environment of each group of peanut seed cultivation samples can be independently adjusted, a good contrast effect is achieved, good drought resistance of cultivated peanut seeds is achieved, soil used in a cultivation experiment can be subjected to unified treatment through the arrangement of the soil preparation box, the influence of external factors on the cultivation experiment is reduced, and the cultivation experiment efficiency is improved. And the cultivation accuracy is improved.

The invention provides a strain of Priestia megaterium M23 and application thereof, belongs to the technical field of agricultural microbial strains, and particularly provides a strain of Priestia megaterium M23 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20232384, and a strain of Priestia megaterium M23 with the preservation number of CCTCC NO: M 20232384 and a strain of Priestia megaterium M23 with the preservation number of CCTCC NO: M 20232384. The strain Pristia megaterium M23 provided by the invention has high salt tolerance and drought tolerance, has the function of promoting plant growth, improves the salt tolerance and drought tolerance of plants, can improve the soluble sugar content of the plants, increases the abundance of soil microorganisms, increases the urease activity of the soil, improves the quality of crop seeds and the like, and has important application value.

The invention relates to a method for creating and identifying excellent drought-enduring germplasm of macadamia nuts. The method comprises the following steps: (1) selecting seeds; (2) seed soaking treatment; (3) chemical mutagenesis; (4) performing ray irradiation mutagenesis; (5) raising seedlings; (6) culturing in a culture medium; (7) identifying selected products; more germplasm with various shapes can be obtained under the combination of chemical mutagenesis and low, high and medium three-level ray irradiation mutagenesis, and then a germplasm culture medium is optimized so as to identify and screen excellent drought-resistant macadamia germplasm; the first culture medium is optimized, meanwhile, the temperature and the impermeability of the culture medium are controlled, and macadimia nut germplasm with the better drought resisting effect can be preferably screened out; the second culture medium is optimized, then small molecule active substances are added, meanwhile, parameters such as impermeability of the culture medium are controlled, macadamia nut germplasm with the better drought resistance effect is further screened out, macadamia nut seedling root growth can be effectively promoted, and the drought resistance of macadamia nut seedlings can be conveniently calculated according to the rooting amount of seedling root systems.

The invention discloses a preparation method of an attapulgite bio-organic fertilizer suitable for tomato growth. The attapulgite bio-organic fertilizer comprises nano attapulgite, an organic composted material, a bio-enzyme and a microbial agent. The invention also discloses a preparation method of the attapulgite bio-organic fertilizer, which comprises the following steps: activating and modifying attapulgite powder with certain purity and granularity to obtain nano attapulgite, fermenting and decomposing organic materials, and proportionally mixing the nano attapulgite and the organic materials with bio-enzyme, microbial flora and the like to obtain the attapulgite bio-organic fertilizer. The attapulgite bio-organic fertilizer obtained by the invention not only can improve the soil structure, but also can provide nutrients, improve the drought tolerance and disease and pest resistance of tomatoes, and enhance the stress resistance of tomatoes; meanwhile, rich macroelements, medium elements and trace elements are provided for growth and development of tomatoes, the yield of tomatoes is increased, and the quality of tomatoes is improved.

The present invention includes modified and unmodified microbes, compositions comprising these microbes, and methods of using these microbes and / or compositions for enhancing plant health, plant growth plant yield and / or drought tolerance. This disclosure also provides non-naturally occurring plant varieties that are artificially infected with microbes descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.

The invention discloses an SV molecular marker remarkably related to drought tolerance and yield of corn and application of the SV molecular marker, and belongs to the field of molecular marker-assisted breeding. According to the invention, the SV variation of the ZmMETTL gene related to the drought tolerance of the corn is found, and the SV variation is a segment of 822bp deletion variation located at the 174690388-174691209 site of the No.3 chromosome of the B73AGPv5 version of the corn. The SV variation is obviously related to the plant height, the single ear weight and the single ear grain weight of a corn material under normal watering and drought conditions, and can be used for screening materials of which the plant height and the yield are obviously different under the normal watering and drought conditions. The invention provides a new direction for identifying corn drought-resistant germplasm resources and assisting corn drought-resistant molecular breeding.

The invention discloses a functional module for regulating drought response of plants and application of the functional module. The functional module comprises TaPYR10 and downstream signal channel members thereof. According to the application disclosed by the invention, a new molecular basis is provided for the drought tolerance of wheat by revealing the effects of the TaPYR10 and downstream partners (TaPP2C30, TaSnRK2.10 and TaNF-YC1) thereof in drought signal transduction. Functional analysis of the genes shows that the genes play a key role in response of plants to drought stress, and stress resistance of the plants can be improved by adjusting related physiological processes (such as stomatal movement, synthesis of osmotic regulation substances and cell active oxygen steady state).

The invention discloses a soybean high-yield close-planting-resistant lodging-resistant drought-resistant gene GmCSLG1 and application thereof. The nucleotide sequence of the soybean GmCSLG1 gene is as shown in SEQ ID NO. 1. The GmCSLG1 gene has multiple effects due to one cause and has the function of improving the drought tolerance, yield, density tolerance and lodging resistance related traits of the soybeans, synergistic improvement of the drought tolerance and yield of the soybeans is achieved through overexpression of the GmCSLG1 gene, and the GmCSLG1 gene can be extensively applied to other plants and crops.

The invention discloses an alpine region cultivation grassland multifunctionality improvement artificial management method comprising the following steps: S1, selecting perennial pasture varieties by comprehensively evaluating the cold resistance, drought tolerance, barren tolerance and nutritional value factors of pasture, and sowing the pasture in an alpine region after variety matching to establish a grassland; s2, in the early stage of grassland establishment, fertilization and microbial agent introduction are conducted regularly; s3, in the grassland steady-state period, fine management is carried out through zoned rotation grazing, introduction of deep-root-system grass seeds and control of the irrigation frequency; s4, gradually reducing external intervention; according to the method, grassland productivity is concerned, multiple functions such as ecological system diversity, carbon circulation and soil health are comprehensively considered, and comprehensive improvement of the multiple functions is achieved. And through scientific staged management measures, the sustainable utilization of the grassland is enhanced, and the ecological function presentation period of the grassland is prolonged. And the method is suitable for complex environmental conditions in alpine regions, and can be popularized and applied in alpine, cold and drought and other fragile ecosystems.