299 results about "Drought tolerance" patented technology

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention discloses application of an OsCPN10a gene in regulation and control of vigor, grain shape and drought tolerance of rice seeds, and belongs to the technical field of rice gene engineering. The invention particularly relates to the research of the OsCPN10a gene in the aspects of regulating and controlling the germination rate, the germination potential, the germination index, the grain length, the grain width, the thousand grain weight, the drought tolerance of the rice seeds and the like. Experimental results show that the grain shape of the OsCPN10a knockout mutant seed is obviously lengthened and widened, and the grain shape of the OsCPN10a overexpression seed is opposite; the yield of the Ocpn10a mutant is higher than that of a TNG67 plant; overexpression of the OsCPN10a has the potential of improving the drought resistance of the rice. The invention reveals that OsCPN10a affects genetic mechanisms such as rice seed vigor, grain length, grain width and storability, provides important gene resources and theoretical basis for genetic improvement of rice, and is beneficial to cultivation of a new rice variety with better grain shape and higher yield.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying drought tolerance of wheat, haplotypes, primers and application. Belongs to the technical field of biological breeding. Research finds that three SNP loci exist on a wheat ARF20-B gene, a wheat drought-enduring haplotype ARF20-BHapI is developed based on the SNP loci, the first locus is located at the 3616bp position of the ARF20-B gene, and the A / G nucleotide polymorphism is A; the second site is located at the 5623bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism; the third site is located at the 9795bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism. The wheat carrying the ARF20-BHapI haplotype has the advantage that the drought tolerance of the wheat carrying the ARF20-BHapI haplotype is higher. The invention further provides a KASP primer group for detecting the ARF20-BHapI haplotype, and successful detection of the drought tolerance of the wheat is achieved.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsSRO1c gene in controlling high-temperature resistance of rice. The OsSRO1c gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsSRO1c gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The gene OsSRO1c for controlling rice high-temperature response is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, high-temperature stress phenotype identification in a seedling stage and an adult-plant stage shows that when a gene segment is deleted, the high-temperature resistance of rice is reduced, and when the expression quantity of the gene is increased, the high-temperature resistance of rice is improved, and the function and the application way of the gene are proved.

The invention discloses application of GmFLZ protein or a coding gene thereof in drought tolerance of plants, in particular to application in drought tolerance of soybeans. The soybean GmFLZ protein or the coding gene thereof provided by the invention has a soybean drought-enduring function. According to the invention, the GmFLZ gene is introduced into soybean roots through an agrobacterium transformation method, so that a GmFLZ gene overexpressed soybean complex plant is obtained, and the drought tolerance of soybeans is remarkably improved.

The invention relates to the technical field of gene engineering, in particular to application of a rice OsSAUR4 gene in regulation and control of rice drought tolerance, and the sequence of the rice OsSAUR4 gene is selected from any one of the following: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a protein sequence as shown in SEQ ID NO.2, and 3) a sequence of which the homology with the nucleotide sequence as shown in SEQ ID NO.1 is more than 90%. The rice OsSAUR4 gene is knocked out, so that the drought tolerance of rice is improved, and the survival rate of rice in a drought stress environment is improved.

Disclosed are a Solanum tuberosum drought tolerance-related transcription factor gene StPIF4 and the use thereof. A CDS sequence of StPIF4 has a length of 1554 bp, has a nucleotide sequence shown as SEQ ID No. 1, and encodes 517 amino acids, the amino acid sequence being shown as SEQ ID No. 2. The gene is located in a cell nucleus. The gene reduces the water loss of Solanum tuberosum seedlings under the drought stress by adjusting the opening degree of stomas, thus enhancing the drought tolerance of Solanum tuberosum. The present invention provides a new genetic material and a theoretical basis for analyzing the drought-tolerant molecular mechanism and drought-tolerant breeding improvement of Solanum tuberosum.

The invention provides an application of a sunflower HaSnRK2 gene in regulating and controlling salt tolerance and drought tolerance of plants, and belongs to the technical field of plant genetic engineering. The invention provides an overexpression vector of a sunflower HaSnRK2 gene. The overexpression vector is applied to regulation and control of salt tolerance and drought tolerance of plants after genetic transformation is carried out on the plants by using a flower dipping method. Through overexpression of the sunflower HaSnRK2 gene, the salt stress tolerance and the drought stress tolerance of plants are remarkably enhanced, and damage caused by reactive oxygen free radicals induced by salt stress and drought stress can be effectively relieved.

A cultivation box for improving drought resistance of peanut seeds comprises a cultivation frame, a plurality of same cultivation chambers are arranged on the cultivation frame, cultivation boxes are detachably connected into the cultivation chambers, a bottom containing box is arranged at the bottom of the cultivation frame, and an electric control box, a soil preparation box and a water tank are arranged in the bottom containing box. According to the cultivation box capable of improving the drought resistance of the peanut seeds, the multiple independent cultivation boxes are arranged in an integrated mode, multiple sets of cultivation experiments can be conducted at a time, the equipment cost is low, the occupied area is small, the cultivation boxes and the cultivation chambers are independently arranged and do not interfere with each other, and the cultivation box is convenient to use. The growth environment of each group of peanut seed cultivation samples can be independently adjusted, a good contrast effect is achieved, good drought resistance of cultivated peanut seeds is achieved, soil used in a cultivation experiment can be subjected to unified treatment through the arrangement of the soil preparation box, the influence of external factors on the cultivation experiment is reduced, and the cultivation experiment efficiency is improved. And the cultivation accuracy is improved.

The invention provides a strain of Priestia megaterium M23 and application thereof, belongs to the technical field of agricultural microbial strains, and particularly provides a strain of Priestia megaterium M23 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20232384, and a strain of Priestia megaterium M23 with the preservation number of CCTCC NO: M 20232384 and a strain of Priestia megaterium M23 with the preservation number of CCTCC NO: M 20232384. The strain Pristia megaterium M23 provided by the invention has high salt tolerance and drought tolerance, has the function of promoting plant growth, improves the salt tolerance and drought tolerance of plants, can improve the soluble sugar content of the plants, increases the abundance of soil microorganisms, increases the urease activity of the soil, improves the quality of crop seeds and the like, and has important application value.

The present invention includes modified and unmodified microbes, compositions comprising these microbes, and methods of using these microbes and / or compositions for enhancing plant health, plant growth plant yield and / or drought tolerance. This disclosure also provides non-naturally occurring plant varieties that are artificially infected with microbes descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.

The invention discloses an SV molecular marker remarkably related to drought tolerance and yield of corn and application of the SV molecular marker, and belongs to the field of molecular marker-assisted breeding. According to the invention, the SV variation of the ZmMETTL gene related to the drought tolerance of the corn is found, and the SV variation is a segment of 822bp deletion variation located at the 174690388-174691209 site of the No.3 chromosome of the B73AGPv5 version of the corn. The SV variation is obviously related to the plant height, the single ear weight and the single ear grain weight of a corn material under normal watering and drought conditions, and can be used for screening materials of which the plant height and the yield are obviously different under the normal watering and drought conditions. The invention provides a new direction for identifying corn drought-resistant germplasm resources and assisting corn drought-resistant molecular breeding.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a wheat gene to regulation and control of plant drought tolerance. The wheat gene provided by the invention is a TaANK-TPR44 gene, and the nucleotide sequence of the TaANK-TPR44 gene is as shown in SEQ ID NO. 1. In order to develop the specific functional characteristics of ankyrin genes from different plant sources, the TaANK-TPR44 gene is over-expressed and transformed into arabidopsis thaliana for functional verification, and the result shows that the drought tolerance of the arabidopsis thaliana with the over-expressed TaANK-TPR44 gene is remarkably improved compared with that of wild arabidopsis thaliana, and the drought tolerance of the arabidopsis thaliana with the over-expressed TaANK-TPR44 gene is remarkably improved compared with that of wild arabidopsis thaliana. Therefore, the TaANK-TPR44 gene plays a positive regulation role in the wheat drought tolerance process, a theoretical basis and gene resources are provided for wheat molecular breeding, and the TaANK-TPR44 gene has very important significance for obtaining drought-tolerant high-yield strains and improving the wheat drought tolerance in the future.

The invention discloses a banana drought-enduring functional gene MaPUB21 and application of the banana drought-enduring functional gene MaPUB21 in improvement of plant drought-enduring capability. A nucleotide sequence of the gene is shown as SEQ ID NO.2. The invention further discloses a method for preparing the banana drought-enduring functional gene MaPUB21. According to the invention, a silence vector (MaPUB21-pTRV) aiming at the MaPUB21 gene is constructed, and an agrobacterium-mediated method is utilized to carry out instantaneous silence in the Musa communis. Drought tolerance analysis shows that after drought stress treatment, compared with a wild type, the content of malonaldehyde, superoxide anions and hydrogen peroxide of the MaPUB21 silent strain is remarkably reduced, and the content of proline and the activity of peroxidase are remarkably improved. The result shows that the drought tolerance of the plant can be effectively improved by silencing the MaPUB21 gene. The gene provided by the invention provides a new resource for plant stress-resistant molecular breeding, and is of great significance for cultivating drought-resistant plants and improving the utilization rate of drought land.

The invention discloses a molecular marker closely linked with a cowpea drought tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea drought tolerance major QTL is DC300099, the KASP type molecular marker 300099 closely linked with the cowpea drought tolerance major QTL is located at the position of 3123287 bp on a chromosome 4, the marker can be used for amplifying a to-be-detected material, and whether a sample carries key allelic variation for controlling drought tolerance or not is judged according to the genotype of the to-be-detected material. By utilizing the molecular marker and the primer thereof provided by the invention, rapid screening of the main effect QTL of drought tolerance of cowpea can be realized, and compared with a traditional phenotype identification method, the molecular marker and the primer thereof have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the cowpea drought-enduring variety and shorten the breeding period.

Provided is a PagHB7a gene related to the salt tolerance and drought tolerance of poplar. The gene is used to create new germplasms of salt-tolerant and drought-tolerant 84K poplar (Populus alba×Populus glandulosa). Being a candidate gene related to the salt tolerance and drought resistance of poplar, the PagHB7a gene provides an important gene resource for breeding new varieties of salt-tolerant and drought-resistant poplar, and can be directly used for molecular-assisted breeding of stress-resistant poplar. In addition, a primer pair for amplifying the candidate gene and a transgenic modification method for poplar are provided, which can evaluate the stress resistance of poplar on the molecular level; on this basis, varieties with remarkable stress resistance can be accurately and efficiently screened, thus effectively shortening the breeding period of poplar.

The invention belongs to the technical field of biology, and particularly relates to Dendrobium officinale DoSTRK1 protein as well as a coding gene and application thereof. The invention discloses a Dendrobium officinale DoSTRK1 protein, which comprises a protein consisting of an amino acid sequence as shown in SEQ ID NO.6, and a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant drought tolerance and is derived from the protein. The invention finds that overexpression of the gene encoding the DoSTRK1 protein can enable an overexpression strain to show stronger tolerance under salt stress of a plant, and specifically, the overexpression strain shows longer roots in a seedling stage and higher chlorophyll content in a growth stage. Under drought stress, the overexpression strain also shows stronger tolerance, and compared with a wild type, the overexpression strain has the advantages that the root length in the seedling stage is increased, and the survival rate in the growth stage is higher.

The invention provides an application of a nitrification inhibitor and biochar as a soil conditioner in enhancing drought resistance of plants, a preparation method of the biochar is as follows: moso bamboo stalks are taken as raw materials, pyrolysis is performed for 4-8 hours under an anoxic state of 250-400 DEG C, soil is red soil, and the plant is polygonatum cyrtonema, and an application method comprises the following steps: adding a nitrogen fertilizer into the soil, the addition amount of the nitrification inhibitor is 1% of the content of nitrogen added to the soil, and the addition amount of the biochar is 3% of the mass of the soil. Through combined application of the biochar and the dicyandiamide, the drought resistance of the polygonatum cyrtonema and the benefits of soil microbial communities are remarkably improved.

The application relates to a rice peroxidase gene OsPRX31 or application of a coded protein thereof in regulating drought resistance of rice, and belongs to the technical field of genetic engineering. OsPRX31 The rice peroxidase gene OsLEA3 participates in drought stress response through an ABA signal pathway, can reduce oxidative stress damage, including reducing active oxygen and malondialdehyde accumulation, improving proline content, and up-regulating drought resistance gene expression, and enhances drought resistance. OsPRX31 Experimental verification shows that rice overexpressing the gene has a higher survival rate after drought, improved physiological indexes, reduced accumulation of oxidized substances, and up-regulated drought resistance gene expression; and knocking out the gene leads to grain abortion, and overexpressing the gene leads to normal seed setting. OsPRX31 OsPRX31 The application can effectively improve the drought resistance of rice and maintain fertility, and provides gene resources and theoretical basis for drought resistance molecular breeding.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

The application discloses OsHAK13 Application of a gene in regulating drought tolerance of rice relates to the technical field of genetic engineering. OsHAK13 The gene encodes an amino acid sequence as shown in SEQ ID NO. 2. OsHAK13 By knocking out the gene through a gene editing technology, a functional loss mutant is obtained, which shows significant enhancement of drought tolerance at a seedling stage. The gene and the mutant can be applied to genetic improvement of crop drought resistance, and provide important gene resources and breeding materials for cultivation of new water-saving and drought-resistant rice varieties, and have important theoretical significance and application value.

This invention relates to the field of microbial technology, specifically to a compound bacterial agent, its preparation method, and its application, wherein the active ingredient is Bacillus arachidica (B. arachidica). Peanut Bacillus CH-RS16 and cold-resistant *Bacillus subtilis* ( Peribacillus frigoritolerans ZM-RS6. The compound microbial agent provided by this invention promotes the growth and development of wheat and improves its drought resistance.

The invention relates to the technical field of microorganisms, in particular to an Exiguobacterium aceticum and application thereof. The Exiguobacterium aceticum strain is classified and named as Exiguobacterium aceticum, the strain number is H102, the Exiguobacterium aceticum strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 24, 2025, and the preservation number is CGMCC No.37157. The Exiguobacterium aceticum strain has the advantages that the Exiguobacterium aceticum strain can be used for preparing the Exiguobacterium aceticum strain; the strain has strong saline-alkaline tolerance, drought tolerance and high temperature resistance, and a foundation is provided for colonization of the strain. Meanwhile, the strain has the functional characteristics of producing acetoin, polysaccharide and polyglutamic acid and the like, can effectively provide nutritional ingredients for plant growth and reduce the harm of abiotic stress to the plants, and therefore, the strain has important application value in the aspects of improving saline-alkali soil, improving abiotic stress resistance of the plants, promoting plant growth and the like.

The invention provides an application of a TaPMT-L gene in improving drought resistance of plants. A nucleotide sequence of the gene is shown as SEQ ID No.1; the invention also provides a specific plant breeding method. Compared with the prior art, the application has the following advantages: the wheat gene TaPMT-L is identified, and overexpression and RNAi interference expression vectors of the wheat gene TaPMT-L are constructed, so that a stably inherited TaPMT-L transgenic wheat material is obtained. Phenotypic and physiological analysis under drought stress treatment shows that the drought tolerance in a TaPMT-L overexpression strain is remarkably enhanced, and the drought tolerance in an RNAi silent strain is reduced, so that the TaPMT-L has a function of positively regulating and controlling the drought resistance in wheat. The drought-resistant regulatory gene which is clear in function and can be stably expressed provides a new molecular tool for genetic improvement of the drought-resistant character of wheat, and has important theoretical value and application prospect for cultivation of new varieties of drought-resistant wheat.

The invention discloses bacillus NJAU-N6 with nitrogen fixation, low temperature resistance and drought resistance and application of the bacillus NJAU-N6, the strain is classified and named as Paenibacillus illinoisis, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35728. The bacillus NJAU-N6 has the advantages that the bacillus NJAU-N6 can be applied to nitrogen fixation, low temperature resistance and drought resistance; the invention discloses the capability of the compound in soil nitrogen fixation and soybean growth promotion. The invention also discloses a microbial inoculum prepared from the strain and application of the microbial inoculum in soil nitrogen fixation and soybean growth promotion. The strain disclosed by the invention has a relatively wide temperature adaptation range and relatively strong drought resistance, can stably play a nitrogen fixation role and a function of promoting soybean growth in a low-temperature and drought-resistant soil environment, and is particularly suitable for planting scenes of crops such as soybeans in semi-arid windy desert areas in northeast China; the method has good popularization and application values in soil fertility improvement and crop yield increase in the region.

The invention discloses a method for synergistically improving drought tolerance of Chinese chestnut plants through earth surface vegetation coverage and chemical regulation and control, and belongs to the technical field of agricultural cultivation. According to the method, the drought-enduring grass seeds with shallow root systems are planted between the Chinese chestnut rows and form layered water utilization with the deep-root Chinese chestnuts, so that water and nutrients competed by herbaceous plants and the Chinese chestnuts are reduced; the ultrahigh grass layer is cut and then covers the rhizosphere of the Chinese chestnuts, biological covering soil moisture conservation is achieved, stress of drought to the Chinese chestnuts is reduced, then the composite drought-resistant agent is applied, abscisic acid in the composite drought-resistant agent can induce pore closing, chitosan can enhance the cell membrane stability, polyaspartic acid can promote water absorption of root systems, and the drought resistance of the Chinese chestnuts is improved. The three components synergistically improve the drought resistance of the Chinese chestnuts, meanwhile, the grass layer mowing period and the compound drought-resistant agent spraying period are staggered from the water demand critical period of the Chinese chestnuts, long-acting drought resistance of the Chinese chestnuts is achieved, negative influences on growth of the Chinese chestnuts and the soil environment are avoided, finally, the irrigation amount is reduced by 50% or above under the moderate drought condition of the Chinese chestnuts, and the yield is increased by 18-25%; the obvious ecological and economic benefits are realized.