182 results about "Drought tolerance" patented technology

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying drought tolerance of wheat, haplotypes, primers and application. Belongs to the technical field of biological breeding. Research finds that three SNP loci exist on a wheat ARF20-B gene, a wheat drought-enduring haplotype ARF20-BHapI is developed based on the SNP loci, the first locus is located at the 3616bp position of the ARF20-B gene, and the A / G nucleotide polymorphism is A; the second site is located at the 5623bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism; the third site is located at the 9795bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism. The wheat carrying the ARF20-BHapI haplotype has the advantage that the drought tolerance of the wheat carrying the ARF20-BHapI haplotype is higher. The invention further provides a KASP primer group for detecting the ARF20-BHapI haplotype, and successful detection of the drought tolerance of the wheat is achieved.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsSRO1c gene in controlling high-temperature resistance of rice. The OsSRO1c gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsSRO1c gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The gene OsSRO1c for controlling rice high-temperature response is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, high-temperature stress phenotype identification in a seedling stage and an adult-plant stage shows that when a gene segment is deleted, the high-temperature resistance of rice is reduced, and when the expression quantity of the gene is increased, the high-temperature resistance of rice is improved, and the function and the application way of the gene are proved.

The invention relates to the technical field of gene engineering, in particular to application of a rice OsSAUR4 gene in regulation and control of rice drought tolerance, and the sequence of the rice OsSAUR4 gene is selected from any one of the following: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a protein sequence as shown in SEQ ID NO.2, and 3) a sequence of which the homology with the nucleotide sequence as shown in SEQ ID NO.1 is more than 90%. The rice OsSAUR4 gene is knocked out, so that the drought tolerance of rice is improved, and the survival rate of rice in a drought stress environment is improved.

The invention belongs to the technical field of biology, and particularly relates to Dendrobium officinale DoSTRK1 protein as well as a coding gene and application thereof. The invention discloses a Dendrobium officinale DoSTRK1 protein, which comprises a protein consisting of an amino acid sequence as shown in SEQ ID NO.6, and a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant drought tolerance and is derived from the protein. The invention finds that overexpression of the gene encoding the DoSTRK1 protein can enable an overexpression strain to show stronger tolerance under salt stress of a plant, and specifically, the overexpression strain shows longer roots in a seedling stage and higher chlorophyll content in a growth stage. Under drought stress, the overexpression strain also shows stronger tolerance, and compared with a wild type, the overexpression strain has the advantages that the root length in the seedling stage is increased, and the survival rate in the growth stage is higher.

The invention provides an application of a nitrification inhibitor and biochar as a soil conditioner in enhancing drought resistance of plants, a preparation method of the biochar is as follows: moso bamboo stalks are taken as raw materials, pyrolysis is performed for 4-8 hours under an anoxic state of 250-400 DEG C, soil is red soil, and the plant is polygonatum cyrtonema, and an application method comprises the following steps: adding a nitrogen fertilizer into the soil, the addition amount of the nitrification inhibitor is 1% of the content of nitrogen added to the soil, and the addition amount of the biochar is 3% of the mass of the soil. Through combined application of the biochar and the dicyandiamide, the drought resistance of the polygonatum cyrtonema and the benefits of soil microbial communities are remarkably improved.

The application relates to a rice peroxidase gene OsPRX31 or application of a coded protein thereof in regulating drought resistance of rice, and belongs to the technical field of genetic engineering. OsPRX31 The rice peroxidase gene OsLEA3 participates in drought stress response through an ABA signal pathway, can reduce oxidative stress damage, including reducing active oxygen and malondialdehyde accumulation, improving proline content, and up-regulating drought resistance gene expression, and enhances drought resistance. OsPRX31 Experimental verification shows that rice overexpressing the gene has a higher survival rate after drought, improved physiological indexes, reduced accumulation of oxidized substances, and up-regulated drought resistance gene expression; and knocking out the gene leads to grain abortion, and overexpressing the gene leads to normal seed setting. OsPRX31 OsPRX31 The application can effectively improve the drought resistance of rice and maintain fertility, and provides gene resources and theoretical basis for drought resistance molecular breeding.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

The application discloses OsHAK13 Application of a gene in regulating drought tolerance of rice relates to the technical field of genetic engineering. OsHAK13 The gene encodes an amino acid sequence as shown in SEQ ID NO. 2. OsHAK13 By knocking out the gene through a gene editing technology, a functional loss mutant is obtained, which shows significant enhancement of drought tolerance at a seedling stage. The gene and the mutant can be applied to genetic improvement of crop drought resistance, and provide important gene resources and breeding materials for cultivation of new water-saving and drought-resistant rice varieties, and have important theoretical significance and application value.

This invention relates to the field of microbial technology, specifically to a compound bacterial agent, its preparation method, and its application, wherein the active ingredient is Bacillus arachidica (B. arachidica). Peanut Bacillus CH-RS16 and cold-resistant *Bacillus subtilis* ( Peribacillus frigoritolerans ZM-RS6. The compound microbial agent provided by this invention promotes the growth and development of wheat and improves its drought resistance.

The invention relates to the technical field of microorganisms, in particular to an Exiguobacterium aceticum and application thereof. The Exiguobacterium aceticum strain is classified and named as Exiguobacterium aceticum, the strain number is H102, the Exiguobacterium aceticum strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 24, 2025, and the preservation number is CGMCC No.37157. The Exiguobacterium aceticum strain has the advantages that the Exiguobacterium aceticum strain can be used for preparing the Exiguobacterium aceticum strain; the strain has strong saline-alkaline tolerance, drought tolerance and high temperature resistance, and a foundation is provided for colonization of the strain. Meanwhile, the strain has the functional characteristics of producing acetoin, polysaccharide and polyglutamic acid and the like, can effectively provide nutritional ingredients for plant growth and reduce the harm of abiotic stress to the plants, and therefore, the strain has important application value in the aspects of improving saline-alkali soil, improving abiotic stress resistance of the plants, promoting plant growth and the like.

This invention discloses a laccase gene PmLAC31 derived from *Pinus massoniana* and its applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the *PmLAC31* laccase gene is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. This invention also provides biomaterials containing the gene, and the application of this gene or biomaterials in improving plant drought resistance and / or lignin content. Experiments show that heterologous overexpression of the PmLAC31 gene in poplar significantly promotes lignin deposition and enhances the plant's drought tolerance. This invention provides important gene resources and application pathways for molecular breeding of forest trees for stress resistance.

The invention belongs to the technical field of biology, and particularly relates to Dendrobium officinale DoORP1 protein and a coding gene and application thereof. The invention discloses a Dendrobium officinale DoORP1 protein, which comprises a protein composed of an amino acid sequence as shown in SEQ ID NO.6, and a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant drought tolerance and is derived from the Dendrobium officinale DoORP1 protein. The invention finds that overexpression of the gene coding the DoORP1 protein enables an overexpression strain to show stronger tolerance under salt stress of a plant, and the overexpression strain specifically shows that the root is longer in a seedling stage and the chlorophyll content is higher in a growth stage. Under drought stress, the overexpression strain also shows stronger tolerance, and compared with a wild type, the overexpression strain has the advantages that the root length in the seedling stage is increased, and the survival rate in the growth stage is higher.

The application provides a Medicago sativa MsNF-YC4 gene and an encoded protein in improving plant stress tolerance. The research of the application shows that overexpression of the MsNF-YC4 gene can promote root growth, regeneration and increase root density of Medicago sativa, and improve drought tolerance, cold tolerance and other stress tolerance of Medicago sativa.

The application provides a Cabernet Sauvignon grape drought-resistant gene VvDREB2A and application thereof, and the nucleotide sequence of the gene is SEQ ID NO:2. The application constructs pRI101-VvDREB2A overexpression and RNAi-VvDREB2A expression inhibition vectors, and verifies the functions by means of Agrobacterium-mediated transformation of grape callus and transient transformation of fruits. Overexpression of VvDREB2A obviously improves the drought resistance of grape callus, and increases the anthocyanin accumulation in grape callus and fruits, and inhibition of the expression affects the drought resistance of grape and the anthocyanin accumulation. The disclosed application is that the gene VvDREB2A is applied to transgenic breeding or callus factory, the drought resistance of plants is improved, and the synthesis and accumulation of grape anthocyanin are improved by regulating flavonoid pathways.

The invention relates to a corn WRKY transcription factor ZmWRKY74 as well as a coding gene and application thereof, and belongs to the technical field of plant gene breeding, the CDS sequence of the coding gene of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.1, the gene negatively regulates the drought tolerance of crop plants, and the amino acid sequence of the transcription factor ZmWRKY74 is as shown in SEQ ID NO.3. Gene positioning analysis is carried out on a drought-resistant mutant of corn, it is found that the ZmWRKY74 gene is an effect gene capable of improving the tolerance of corn to drought stress, and through a ZmWRKY74 gene overexpression experiment, the negative regulation effect of the ZmWRKY74 gene on the drought tolerance of corn plants is verified; important theoretical significance and application value are provided for creation of new corn germplasm and molecular breeding of corn.

The invention relates to the technical field of agriculture and genetic engineering, and particularly discloses application of a SibZIP11 gene in regulation and control of drought tolerance of millet, and the nucleotide sequence of the SibZIP11 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the SibZIP11 gene. According to the invention, the transgenic millet strain is constructed by knocking out the millet SibZIP11 gene, so that the drought tolerance of millet is enhanced.

The invention relates to the technical field of genetic breeding, and particularly provides a gene related to drought tolerance of pear trees and application of the gene, the gene is specifically a PbAREB3 gene, the nucleotide sequence of the gene is shown as SEQ ID NO.1, and the amino acid sequence of the gene is shown as SEQ ID NO.2; the PbAREB3 gene provided by the invention can be used for effectively judging the drought tolerance of various pear tree materials, and the PbAREB3 gene with high expression quantity is high in corresponding drought tolerance; according to the gene, the screening efficiency can be effectively improved, and meanwhile, a guidance thought can be effectively provided for cultivating a drought-resistant pear tree transgenic material.

This invention relates to the field of biotechnology, specifically to... StNCED3 The application of genes in improving the abiotic resistance of potatoes: This invention involves constructing... StNCED3 The screening and identification of gene overexpression vectors and transgenic lines, and their application in improving potato resistance to drought and low-temperature stress, revealed the overexpression of... StNCED3 The gene can significantly enhance the drought and cold resistance of potato plants, providing core functional genes and high-quality breeding materials for molecular breeding of potatoes for drought and cold resistance. It effectively solves the technical bottlenecks of weak drought and cold resistance of the current main potato varieties and unstable yield in arid and low-temperature production areas, and has important theoretical research value and industrial application prospects.

This invention discloses a method and application for improving wheat drought stress resistance based on the transcription factor TaHY5, relating to the fields of plant genetic engineering, wheat genetic breeding, and crop stress resistance improvement. This invention involves constructing a TaHY5 overexpression vector or a CRISPR / Cas9 editing vector, and transforming wheat immature embryos / callus tissue using Agrobacterium-mediated transformation or gene gun methods to obtain transgenic and gene-edited materials. After drought stress treatment, indicators such as biomass, malondialdehyde (MDA) content, and electrolyte permeability are measured to comprehensively evaluate drought resistance. Results show that TaHY5 overexpression significantly increases wheat biomass, APX, CAT and other antioxidant enzyme activities, as well as soluble protein and GSH content under drought stress, while reducing MDA accumulation and electrolyte permeability, and enhancing photosynthetic maintenance, reactive oxygen species scavenging, osmotic regulation, and cell membrane stability. This invention is the first to demonstrate that TaHY5 can synergistically improve wheat drought resistance, providing a simple and comprehensive improvement scheme, and offering new gene resources and practical technical pathways for wheat drought-resistant breeding.

The application belongs to the technical field of crop molecular breeding, and particularly relates to application of rice OsGLP3-7 gene in regulation of drought tolerance of rice seedling stage. The application proves for the first time that the rice gene OsGLP3-7 is a functional gene for regulating drought tolerance of rice seedling stage, and cloning and biological function verification of the gene have important reference significance for the study of molecular mechanism of drought tolerance of rice seedling stage. The characteristics of the OsGLP3-7 gene and the protein coded by the gene in regulation of drought tolerance of rice seedling stage are utilized, and a genome targeting modification technology such as CRISPR / Cas9 is adopted to screen the strain with improved drought tolerance of rice seedling stage by mutating the nucleotide sequence of the gene, and the application has very important application in agricultural production. It is indicated that the rice OsGLP3-7 gene as a breakthrough point can provide an efficient breeding mode for creating rice varieties, germplasm resources and hybrid rice parents with enhanced drought tolerance of seedling stage.

This invention provides a nitrate reductase gene and its application as a target for regulating plant stress resistance. The invention discloses a novel nitrate reductase gene, named NR1.2, that regulates nitrogen use efficiency in plants. NR1.2 is closely related to drought tolerance in plants; reducing the expression / activity of NR1.2 in plants results in decreased seedling height and increased drought tolerance. Therefore, NR1.2 can be used as a target for regulating drought tolerance in plants, and materials or methods for downregulating NR1.2 can be applied to improve plant varieties.

The application discloses a small peptide SP07 for regulating drought resistance of crops and application thereof, and the amino acid sequence of the small peptide SP07 is shown as SEQ ID NO. 1. The small peptide SP07 is obtained by a chemical synthesis method, seedling stage corn in drought is treated by spraying, and the survival rate of the corn is significantly improved compared with a control. The small peptide SP07 is sprayed in a field under normal growth and drought environment, the flowering period of the corn is advanced, and the yield-related traits of the corn are significantly improved. In addition, wheat under drought stress can be promoted to flower in advance by spraying the small peptide SP07, and the small peptide SP07 can be used for improving drought resistance of crops.

The invention discloses a medicago sativa calcium-dependent protein kinase CDPK33 gene and an application thereof. According to the invention, a cDNA (complementary deoxyribonucleic acid) sequence of MsCDPK33 is cloned from medicago sativa, and the MsCDPK33 gene responds to induction of cold and drought. Researches find that interference on expression of the CDPK33 gene in alfalfa can improve the growth performance, cold tolerance and drought tolerance of plants. The invention provides a method for cultivating high-quality, cold-resistant and drought-resistant alfalfa by using the CDPK33 gene, and after the gene is interfered in alfalfa, the growth performance, cold resistance and drought resistance of transgenic alfalfa are improved; meanwhile, after the gene is over-expressed in medicago sativa, the growth performance, cold resistance and drought resistance of transgenic medicago sativa are reduced.

The application discloses application of a Pyrus betulaefolia E3 ubiquitin ligase gene PbrATL18 in genetic improvement of plant drought resistance and anthracnose resistance and belongs to the technical field of genetic engineering. The nucleotide sequence of the PbrATL18 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The plant drought resistance and / or anthracnose resistance performance is improved by overexpressing the PbrATL18 gene in the plant or improving the protein activity. The application constructs a gene overexpression Arabidopsis transformation vector and a gene silencing P. betulaefolia seedling transformation vector respectively, obtains positive plant seedlings, and carries out drought and anthracnose treatment. The results show that overexpression of the E3 ubiquitin ligase gene PbrATL18 can effectively maintain the balance of active oxygen in cells, keep the cell osmotic potential stable, and significantly improve the plant drought resistance and anthracnose resistance performance, thereby providing a new idea for high drought tolerance and disease resistance breeding of pear trees.

The application discloses a gene PtoTAR2 for improving drought resistance and sensitivity to ABA of poplar and application thereof, a nucleotide sequence of the gene is shown in SEQ ID NO. 1, an amino acid sequence of a coded protein is shown in SEQ ID NO. 3, the gene can negatively regulate drought resistance and sensitivity to ABA of the poplar, provides a candidate gene for cultivating new germplasm of the poplar with improved stress resistance and without affecting growth, and effectively shortens a breeding cycle. The application also discloses a method for improving drought resistance and sensitivity to ABA of the poplar and related application, and the drought resistance and the sensitivity to ABA of the poplar are specifically enhanced by silencing expression of the gene PtoTAR2 in the poplar, which provides an effective way for cultivating new germplasm of the poplar with improved stress resistance and without affecting growth, and has important significance for promoting sustainable development of forestry.

This application discloses a Penicillium strain with stress resistance, growth promotion, and insecticidal functions, and its applications. The preservation number of this Penicillium strain is CGMCC No. 42545. This Penicillium strain naturally integrates four functions: salt tolerance, drought tolerance, plant growth promotion, and insecticidal activity against lepidopteran pests. It can grow normally in a 15% NaCl concentration medium, significantly improving plant survival rate under drought stress. Simultaneously, it promotes growth indicators such as root length and root fresh weight in crops like peas. It exhibits highly effective lethal activity against young larvae of lepidopteran pests such as diamondback moth and beet armyworm, and has high safety against non-target organisms. This Penicillium strain can be prepared into solid or liquid inoculants, showing promising application prospects in stress resistance, growth promotion, and pest control for crops grown in saline-alkali / arid areas. It realizes an integrated solution of "stress adaptation - crop growth promotion - pest control," replacing the combined use of multiple single-function agents and reducing agricultural production costs.

The invention relates to the technical field of crop cultivation, in particular to a cultivation device for corn variety drought tolerance research. A drought resistance testing mechanism; the linkage monitoring mechanism is used for burying different varieties of corn seeds in soil of a plurality of corn seed grooves in the process of researching the drought tolerance of corn varieties, corresponding transmission screws are synchronously driven to rotate through four micro motors, and in the synchronous rotation process of the four transmission screws, the four transmission screws are driven to rotate synchronously. The lifting sliding blocks in threaded connection with the outer sides of the four transmission screws move in the vertical direction, due to the fact that the front vertical sliding block and the rear vertical sliding block are alternately arranged up and down, the heating lamps on the front row of lifting arms and the rear row of lifting arms have a height difference all the time in the working process, and the row of heating lamps close to soil can form a high-strength arid area; and a low-intensity arid area is formed on the row at the higher position, so that researchers can synchronously observe and compare the growth difference of different corn varieties under the gradient drought condition in the same experimental period.

The invention relates to the technical field of forest tree breeding and cultivation, in particular to an integrated method for improved variety breeding, rapid propagation and container seedling cultivation of poplar pie suitable for semi-arid regions. The integration method comprises the following steps: hybridizing populus arabinosa or populus sinkiang as a female parent and populus tomentosa or populus arabinosa as a male parent, screening by using a molecular marker to obtain F1-generation plants with salt-tolerant and drought-resistant characteristics, and sequentially carrying out rapid propagation, container seedling cultivation and afforestation planting on the F1-generation plants. Compared with the prior art, the method can solve the problem of rooting of the poplar pie, the afforestation survival rate is remarkably increased, and the method has the advantages of shortening the breeding period and accurately improving the stress resistance.