101 results about "Bacillus thuringiensis kurstaki" patented technology

The invention relates to the field of agricultural microbial preparations, in particular to a complex microbial inoculant sustained-release granule for preventing and treating sweet potato stem nematode disease and a preparation method of the complex microbial inoculant sustained-release granule. Comprising the following components in parts by weight: 15-25 parts of paecilomyces lilacinus viable bacterium powder, 10-20 parts of bacillus thuringiensis viable bacterium powder, 10-20 parts of streptomyces viable bacterium powder, 5-15 parts of bacillus subtilis viable bacterium powder, 15-25 parts of charcoal, 5-15 parts of sodium alginate, 5-15 parts of humic acid, 5-10 parts of chitosan, 3-8 parts of calcium silicate, 1-5 parts of mannitol and 1-5 parts of sodium lignin sulfonate. Four microorganisms with complementary functions, namely paecilomyces lilacinus, bacillus thuringiensis, streptomycete and bacillus subtilis, are adopted, the sweet potato stem nematode disease is synergistically controlled through multiple action mechanisms, and the control effect and the stability are remarkably improved.

The invention discloses a bacillus thuringiensis L3 and an application thereof. The preservation number of the bacillus thuringiensis L3 is CCTCC (China Center for Type Culture Collection) NO: M 2025487. The gene sequence of the 16S rDNA of the bacillus thuringiensis L3 is as shown in SEQ ID NO: 1. Under different adversity stress conditions, the bacillus thuringiensis L3 shows a certain stress resistance potential. Research finds that the bacillus thuringiensis L3 has certain acid resistance, high temperature resistance, cholate resistance and drought resistance, and has strong alkali resistance.

The invention discloses a chitinase mutant obtained based on random mutation as well as a preparation method and application of the chitinase mutant. On the basis of an error-prone PCR (Polymerase Chain Reaction) technology, the mutant ChiA-7 (T165I / C253Y / A391T / N480S / T508S / K547T / I550V / N607T) of the chitinase ChiA of the bacillus thuringiensis is obtained by the invention. Compared with ChiA, the ChiA-7 has better stability, and the enzyme activity of the ChiA-7 is about 90% of the initial enzyme activity after the ChiA-7 is subjected to heat preservation for 3 hours at 40 DEG C in a phosphate buffer solution with the optimal catalysis condition of 40 DEG C and the pH value of 4.0, while the enzyme activity of the ChiA-7 is attenuated to 75% of the initial enzyme activity. The chiA-7 is connected with a carrier pET20b (+) and is transferred into escherichia coli Escherichia coli BL21 (DE3), and the extracellular enzyme activity of a recombinant bacterium reaches 10.23 U / mL, which is improved by 13.41% compared with the enzyme activity of a control bacterium for expressing chiA. The chitinase mutant obtained by the preparation method disclosed by the invention has good thermal stability and enzymatic activity, and can be applied to hydrolysis of chitin and inhibition of mould.

The invention provides a bacillus thuringiensis strain, a fermentation culture method and application of the bacillus thuringiensis strain. The strain is preserved in the China Center for Type Culture Collection on September 28, 2011, the preservation address is No. 299 on eight road, Wuchang District, Wuhan City, Hubei Province, the preservation number is CCTCC No: M 2011334, and the preservation date is September 28, 2011. The strain cultured by the fermentation method has efficient, specific and stable insecticidal activity on culex, aedes and other mosquitoes, has the advantages of low preparation cost, environmental friendliness and the like, can be developed into a biological insecticide for preventing and killing mosquitoes and preventing and controlling malaria, dengue fever, chikungunya and other mosquito-borne infectious diseases, and meets the requirements of green and safe prevention and control in China.

The invention discloses a bacillus thuringiensis CMS-BT17, which is classified and named as bacillus thuringiensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC 35313, and is preserved on July 21, 2025. The bacillus thuringiensis strain CMS-BT17 directly separated and screened from a zizania aquatica soil sample in Haikou City of Hainan carries four insecticidal protein coding genes of Cry8Ca, Cry8Da, Spp1Aa and Vpb4Ca, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting tomato fungal diseases, can be widely applied to prevention and control of agricultural diseases and pests, and has a wide application prospect. The sustainable development of green agricultural production is promoted.

The invention relates to the technical field of agricultural biological prevention and control, and discloses a biological prevention and control complex microbial inoculant and application thereof.The biological prevention and control complex microbial inoculant is prepared by the steps that natural bio-based compounds vanillin and vanillic acid serve as main raw materials, and a biodegradable trialdehyde monomer is designed and synthesized; a biodegradable trialdehyde monomer and p-phenylenediamine are used as organic building units to assemble and form an ordered porous crystal structure, and the biodegradable COF material is obtained; the preparation method comprises the following steps: loading a basic complex microbial inoculant consisting of bacillus thuringiensis, bacillus subtilis and streptomyces willerigensis on a biodegradable COF material by adopting an impregnation adsorption method, and coating the surface of the biodegradable COF material with tetraethylenepentamine and tannic acid through copolymerization to form a degradable polytannic acid layer, so as to prepare the slow-release complex microbial inoculant, and the effective active components of the slow-release complex microbial inoculant can be slowly released; the lasting period and the utilization rate of the composite microbial agent can be remarkably improved, and the composite microbial agent shows an obvious inhibition effect on pathogenic bacteria such as fusarium oxysporum and botrytis cinerea and can be applied to prevention and treatment of plant diseases.

New insecticidal proteins, nucleotides, peptides, their expression in plants, methods of producing the peptides, new processes, production techniques, new peptides, new formulations, and new organisms, a process which increases the insecticidal peptide production yield from yeast expression systems. The present invention is also related and discloses selected endotoxins we call cysteine rich insecticidal peptides (CRIPS) which are peptides derived from Bacillus thuringiensis (Bt) and their genes and endotoxins in combination with toxic peptides known as Inhibitor Cystine Knot (ICK) genes and peptides as well as with other types of insecticidal peptides such as trypsin modulating oostatic factor (TMOF) peptide sequences used in various formulations and combinations; of both genes and peptides, useful for the control of insects.

This application relates to the field of biological control, specifically disclosing a compound bacterial agent containing Bacillus thuringiensis, its preparation method, and its application. A method involves fermenting Bacillus thuringiensis HP-SYJ2 to a concentration of (3-8) × 10⁻⁶. 9 A Bacillus thuringiensis HP-SYJ2 bacterial suspension was obtained by mixing cfu / ml with hydroxyapatite and biochar, then mixing the mixture with the Bacillus thuringiensis HP-SYJ2 bacterial suspension, soaking, and air-drying until the moisture content is 30-40%, followed by maturation for 1-2 weeks to obtain the loading material. Fenugreek seed oil, emulsifier, soil penetrant, and water were mixed and sheared to obtain an emulsion with a fenugreek seed oil concentration of 0.1-0.5 wt%. The loading material and emulsion were packaged separately and mixed before application. This compound microbial agent can be used for the prevention and treatment of root rot in medicinal herbs or to promote their growth. It has the advantages of both preventing and treating root rot in medicinal herbs and promoting their growth.

The application discloses a floating composite microbial preparation for removing marine oil pollution and a preparation method thereof. The floating composite microbial preparation contains bacillus thuringiensis NH26 strain, hollow glass microbeads and a lipopeptide surfactant purified from a fermentation liquor of the NH26 strain, has floating characteristics, can rapidly activate and efficiently degrade oil hydrocarbon, has good degradation capacity for alkanes and polycyclic aromatic hydrocarbons in seawater, and still has good degradation capacity for alkanes in seawater containing high-concentration oil hydrocarbon. The floating composite microbial preparation can be used for removing marine oil pollution and repairing marine environment. In addition, the application further provides a preparation method of the floating composite microbial preparation. The preparation method has simple preparation process, the obtained floating composite microbial preparation has uniform particle size, has high embedding rates for nitrogen and phosphorus, and can be prepared on a large scale.

The invention belongs to the technical field of high-temperature koji making and wine brewing, and particularly relates to traditional Chinese medicine-containing anti-koji high-temperature yeast and a preparation method thereof, the raw materials comprise 90-100 parts of wheat, 8-10 parts of mother yeast, 2-4 parts of traditional Chinese medicines, and 0.5-1.5 parts of a complex microbial inoculant; wherein the traditional Chinese medicine comprises at least one of tripterygium wilfordii, radix sophorae flavescentis and mint; the complex microbial inoculants comprise bacillus thuringiensis, bacillus sphaericus, beauveria bassiana and metarhizium anisopliae. The traditional Chinese medicines and the complex microbial inoculant are added into the raw materials, so that the high-temperature yeast has the effects of avoiding, refusal to eat and killing the stomach on yeast insects on the premise of not generating toxic substances to influence food safety, and can also endow the high-temperature yeast with special traditional Chinese medicine fragrance, and the content of flavor substances in the high-temperature yeast is increased.

The invention provides a soil remediation agent as well as a preparation method and application thereof, and relates to the technical field of soil remediation. The soil remediation agent disclosed by the invention is prepared from 15 to 20 parts of a microbial agent, 20 to 25 parts of humic acid, 15 to 20 parts of fulvic acid, 5 to 10 parts of a soybean meal extract, 1 to 1.5 parts of a seaweed extract and 20 to 25 parts of a plant extract, wherein the microbial agent comprises an inner core composed of straw biochar and a compound bacteria solution, and a shell formed by methoxypolyethylene glycol modified chitosan, sodium caseinate and sodium tripolyphosphate; the composite bacterial liquid comprises a bacillus thuringiensis bacterial liquid and a beauveria bassiana bacterial liquid in a volume ratio of 10: (5-8). The soil remediation agent can improve the soil structure, relieve acidification and hardening, effectively resist diseases for a long time, fundamentally improve the physicochemical properties and micro-ecological environment of the ginger continuous cropping soil, and realize comprehensive remediation.

This application relates to the field of microbial strain technology, specifically disclosing a Bacillus thuringiensis HP-SYJ2 strain and its applications. The Bacillus thuringiensis HP-SYJ2 strain is deposited at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO: M2025343, deposit date: March 3, 2025, address: Wuhan University, Wuhan, China. The strain of this application can be used to prepare insecticides for controlling underground pests, which is of great significance for the biological control of plant diseases and pests.

A pest control composition includes humic acid and an emulsion polymer. The emulsion polymer includes including methyl methacrylate, butyl acrylate, acrylic acid and vinyl toluene. The pest control composition also includes bacillus thuringiensis and water.

The invention relates to a complex microbial inoculant for mixed compost of sugarcane filter mud and sugarcane leaves and an application method, and belongs to the technical field of microorganisms, the complex microbial inoculant comprises a high-temperature microbial inoculant, and the high-temperature microbial inoculant is prepared from thermophilic carbon monoxide streptomyces G-4-7, brevibacillus brevis G-5-2 and thermophilic chaetomium; the medium-temperature microbial inoculum is prepared from trichoderma longibrachiatum, staphylococcus warneri LH-403 and bacillus thuringiensis LMU-81, and the medium-temperature microbial inoculum is prepared from the trichoderma longibrachiatum and the staphylococcus warneri LH-403; the complex microbial inoculant adopts a two-stage inoculation method. The complex microbial inoculant can effectively enhance degradation of lignocellulose so as to promote compost maturity, the humification degree of compost is further improved through the biological enhancement effect, the nitrogen fixation effect of the compost is facilitated, the germination index of the compost is improved, resource utilization of organic matter rich in lignocellulose can be promoted, and the environment-friendly effect is achieved. And a technical basis is provided for a green treatment mode of agricultural wastes.

The invention discloses a microbial preparation for preventing and treating plant root-knot nematode as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations. The microbial preparation for preventing and treating the plant root-knot nematode is of a double-layer coating structure and comprises an inner layer and an outer layer, the inner layer comprises phaffia rhodozyma fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and an inner layer carrier material; the outer layer comprises a streptomyces densely-rotating fermentation liquid and an outer layer carrier material; according to the application, the phaffia rhodozyma, the paecilomyces lilacinus, the bacillus subtilis, the bacillus thuringiensis fermentation liquor, the beauveria bassiana fermentation liquor and the streptomyces dense are compounded, and the strains have a synergistic effect, so that functional complementation is realized; a double-layer coating structure is adapted to acid soil, guarantees the initial activity of the strain and improves the survival efficiency of the strain on one hand, and can prolong the shelf life and the function exerting period on the other hand, so that long-acting pest control is realized.

The application discloses a kind of orthogonal linear gene expression system and application, belong to genetic engineering field.The application is with Bacillus thuringiensis as expression host, realizes the stable expression of gene by constructing linear plasmid in cell.Meanwhile, the application also realizes the accurate control of linear plasmid copy number.This not only develops a stable protein expression system in Bacillus thuringiensis, but also lays a foundation for Bacillus thuringiensis synthetic biology application.

The invention discloses bacillus thuringiensis, a microbial agent, a composite insecticide and application thereof, and belongs to the field of microorganisms and fermentation engineering. The bacillus thuringiensis BT001 is separated from dead sick noctuid, and the bacillus thuringiensis BT001 is high in toxicity and wide in insecticidal spectrum, has certain salt resistance, heavy metal ion resistance, alkali resistance and high temperature resistance, and also has certain tolerance to ultraviolet rays; the insecticidal composition can tolerate a variety of pesticides, and can be compounded with a variety of pesticides for use to synergistically improve the insecticidal effect; the bacillus thuringiensis BT001 disclosed by the invention also has a growth promoting effect at the same time. Therefore, the bacillus thuringiensis BT001 disclosed by the invention can be used for preventing and treating agricultural insect pests and has an important application prospect.

The application provides a degrading bacteria agent for alkane and polycyclic aromatic hydrocarbon in high-salt water body and application thereof. The bacteria agent contains Bacillus thuringiensis NH26 strain, and is prepared by activating, fermentation culture, bacteria body collection, suspension preparation and freeze-drying of the NH26 strain. The Bacillus thuringiensis NH26 bacteria agent prepared by the application has excellent degradation capacity for alkane and polycyclic aromatic hydrocarbon, for example, the total degradation rate of 20 g / L diesel oil is 90.2%, and the total degradation rate of 50 g / L diesel oil is 84.8%. In addition, the degrading bacteria agent of the application also has strong degradation capacity for polycyclic aromatic hydrocarbon in seawater, for example, the removal rate of 50 mg / L phenanthrene in seawater is 68.56%, and the removal rate of 100 mg / L phenanthrene is 63.47%, so that the bacteria agent of the application can be applied to repair high-salt water body polluted by alkane and polycyclic aromatic hydrocarbon.

The invention provides a bacillus thuringiensis engineering strain for efficiently preventing and treating plutella xylostella, the engineering strain carries a nucleic acid fragment for preparing dsRNA, and the prepared dsRNA can interfere with the expression of a plutella xylostella mitogen activated protein kinase (MAP4K4) gene; the bacillus thuringiensis engineering strain is a Bt strain B-T-3 of which the preservation number is CCTCC (China Center for Type Culture Collection) No: M20252327. The engineering bacterium provided by the invention has a strong silencing effect on plutella xylostella MAP4K4 gene expression and shows high insecticidal activity on plutella xylostella larvae, the median lethal concentration on the plutella xylostella larvae is 2.3 * 10 < 6 > cfu / mL, and the activity is 3.2 times that of a wild strain. The result shows that the dsRNA and the Bt strain have synergistic toxicity.

The present application relates to a kind of bacillus thuringiensis for preventing and treating spodoptera exigua and potato tuber moth.The bacillus thuringiensis is preserved in China Microorganism Culture Collection Center, and the preservation number is CGMCC No. 38161.

The invention discloses bacillus thuringiensis CMS-5E8, which is classified and named as bacillus thuringiensis, and is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, the preservation number is CGMCC 35311, and the preservation date is July 21, 2025. The strain carries 16 insecticidal protein coding genes, namely Cry1Aa, Cry1Ah, Cry1Be, Cry1If, Cry1Na, Cry1Ea, Cry1La, Cry2Ab, Cry2Ah, Vip3Aa, Vip3Ad, Spp1Aa, Vpb4Ca, Tpp78Ba, Mpp46Ab and Mpp3Aa, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting fungal diseases, and can be widely applied to prevention and treatment of agricultural diseases and insect pests.

The invention discloses a method for promoting decomposition of liquid manure and application, and relates to the technical field of biology. The method comprises the following steps: inoculating a microbial combination in the liquid manure to carry out decomposition treatment; the microbial composition is prepared from bacillus subtilis, lactobacillus plantarum, saccharomyces cerevisiae and bacillus thuringiensis, the microbial composition is prepared from bacillus subtilis, lactobacillus plantarum, saccharomyces cerevisiae and bacillus thuringiensis, and the microbial composition is prepared from bacillus subtilis, lactobacillus plantarum, saccharomyces cerevisiae and bacillus thuringiensis. According to the method, the decomposition efficiency of the liquid manure can be greatly improved, the treatment method is convenient to operate, complex equipment is not needed, the method is suitable for a large-scale breeding scene, resource utilization of the liquid manure is achieved, the risk of environmental pollution is reduced, and good economic, ecological and social benefits are achieved.

The present application belongs to the field of microbial pesticides, and relates to a bacillus megaterium NBNC-104 for preventing and treating root-knot nematodes, a compound microbial agent and application thereof. The preservation number of the bacillus megaterium strain NBNC-104 is CCTCC M 20242324. Laboratory bioassay data show that the bacillus megaterium NBNC-104 has high toxic killing activity and repellent effect on southern root-knot nematodes, and has synergistic nematode killing activity after being compounded with bacillus thuringiensis strain NBIN-863 and kunming streptomyces sp. NBNH-006, and the strain can effectively colonize in the rhizosphere soil environment within 30 days under potting conditions. Therefore, the strain has broad application prospects in agricultural production.

The invention relates to bacillus thuringiensis ZKSW003 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The bacillus thuringiensis ZKSW003 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36074, the preservation date is September 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention further provides application of the bacillus thuringiensis ZKSW003 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The bacillus thuringiensis ZKSW003 disclosed by the invention not only can promote the growth of crops and realize weight loss and efficiency improvement, but also can control the occurrence of soil-borne fungus and bacterial diseases, and can inhibit the growth of root-knot nematode. Chemical pesticide application can be reduced, pesticide residues are reduced, and sustainable development of agricultural economy is promoted.

The application provides a camellia plant endophytic bacteria, a bacterial agent thereof and an anti-influenza virus A application; the camellia plant endophytic bacteria is Bacillus thuringiensis, which is preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No.32945. The fermentation product of the strain shows good antiviral activity in in vitro and in vivo experiments, can effectively inhibit the replication of influenza virus, and protect infected mice from lethal influenza virus; also shows the effect of regulating immune response, enhances the secretion of IFN-gamma by down-regulating the secretion of pro-inflammatory factors such as IL-6 and IL-10, thereby improving the immune resistance of the body.

The invention belongs to the technical field of microbial complex microbial inoculants and fermentation, and particularly relates to a preparation method and application of an ultraviolet stress resistant phycomycetes complex microbial inoculant, and the phycomycetes complex microbial inoculant is prepared by mixing bacillus thuringiensis NBIN-863 and microcoleus with the preservation number of CCTCC NO: M 20251992. According to the microbial agent, the ultraviolet resistance is enhanced through phycomycete metabolism interaction, the microcoleus provides physical shielding and antioxidant protection, and the bacillus secretes nematode killing substances, so that the dual effects of preventing and treating root-knot nematodes and improving soil nutrients are achieved. The microbial agent is expected to be used for preparing metabolite micromolecular acid 3-methylthiopropionic acid for inhibiting pests through fermentation.

The invention relates to bacillus thuringiensis for preventing and treating spodoptera frugiperda. The bacillus thuringiensis strain is IPPO68 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.37126, and the exopolysaccharide produced by the bacillus thuringiensis strain has insecticidal activity on spodoptera frugiperda.

The invention discloses a miR-13a-3p simulant, a biological reagent and application. The miRNA simulant is composed of a positive-sense strand with the sequence shown as SEQ ID NO: 1 and an antisense strand with the sequence shown as SEQ ID NO: 2. The simulant has biological functions consistent with miR-13a-3p, and can effectively realize negative regulation of the beta GRP gene; the bacillus thuringiensis toxic protein synergist can be introduced into lepidoptera pests for use, so that the insecticidal activity on the lepidoptera pests is effectively improved, and a new thought is provided for prevention and control of agricultural pests.

The invention discloses a complex microbial inoculant for preventing and treating carya illinoensis perwing moths and application of the complex microbial inoculant, and belongs to the technical field of pest prevention and treatment. The complex microbial inoculant for preventing and treating the carya illinoensis pernicifuge is prepared from the following raw materials in parts by mass: 2 to 3 parts of beauveria bassiana bacterial liquid, 3 to 5 parts of bacillus thuringiensis bacterial liquid, 4 to 8 parts of brevibacillus laterosporus bacterial liquid, 5 to 6 parts of bacillus cereus bacterial liquid, 8 to 10 parts of sodium alginate, 3 to 5 parts of seaweed extract, 1 to 2 parts of liquorice root extract and 3 to 4 parts of soybean extract. The complex microbial inoculant disclosed by the invention has an excellent prevention and treatment effect on the perfond moth, and the problems that the existing chemical agent is easy to generate drug resistance and pesticide residue, destroys ecological balance, threatens food safety and human health and the like are solved.

The application provides an application of Camellia nitidissima endophytic Bacillus thuringiensis in preparation of a medicine for relieving ulcerative colitis, the Camellia nitidissima endophytic Bacillus thuringiensis is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.32945. The Bacillus thuringiensis in the application can treat ulcerative colitis through a multi-target synergistic mechanism of regulating intestinal flora, activating an aromatic hydrocarbon receptor AhR, repairing an intestinal barrier, regulating a metabolic pathway and the like, so as to solve the problems of large side effects and easy recurrence of traditional treatment methods.