351 results about "Paecilomyces" patented technology

The invention provides a special bacterial manure for peanut planting, which comprises the following components in parts by weight: 5-25 parts of a compound microbial agent, 30-65 parts of dreg composite powder, 5-20 parts of starch and 10-25 parts of bentonite, wherein the compound microbial agent is composed of bacillus subtilis agent, a rhodopseudomonas palustris agent, a trichoderma harzianum agent and a paecilomyces lilacinus agent; the mass ratio of the bacillus subtilis agent to the rhodopseudomonas palustris agent to the trichoderma harzianum agent to the paecilomyces lilacinus agent is 5:2:1:2, and the total effective viable count of the compound microbial agent is10 billion cfu/g. The bacterial manure is mainly used as a base fertilizer, and can change the microenvironment around the roots of peanuts to prevent various plant diseases and insect pests while promoting nutrient absorption of the peanuts, so that the yield and quality of the peanuts are improved.

The invention relates to an antibacterial mildewproof polylactic acid composition and a preparation method thereof. The composition comprises the following mixed components: polylactic acid, an antibacterial mildewproof agent, and a dispersant; wherein on a basis of 100 parts by weight of polylactic acid, the composition comprises 0.3-2 parts by weight of antibacterial mildewproof agents, and 0-0.5 parts by weight of dispersants; the antibacterial mildewproof agent is a polyguanidine pyrithione antibacterial mildewproof agent which is obtained by mixing an aqueous solution of water-soluble polyguanidine inorganic acid salts or organic acid salts and an aqueous solution of sodium pyrithione; the molar ratio of the water-soluble polyguanidine inorganic acid salts or organic acid salts and sodium pyrithione is 1:(0.1-5). The polylactic acid composition of the invention has good antibacterial and mildewproof effect. The antibacterial effect on staphylococcus aureus and escherichia coli is up to 99.9%; the mildewproof grade for mold such as aspergillus niger, aspergillus terreus, paecilomyces varioti bainier, and the like reaches 0 grade; the composition has good water resistant stability, and has wide application prospects.

The invention provides a multifunctional active organic fertilizer and a preparation method thereof, relating to an organic fertilizer containing bacteria, fungi and spores, particularly an active organic fertilizer with functions of soil restoration and disease/pest resistance. The invention aims to provide a multifunctional active organic fertilizer capable of restoring soil pollution and improving product quality and a preparation method thereof. The multifunctional active organic fertilizer is composed of three layers from inside to outside, wherein the innermost layer is composed of a component A of which the main effective components are Bacillus subtilis and Paecilomyces lilacinus; the middle layer is composed of a component B and a component C containing multiple rich nutritive elements; and the outermost layer is composed of a component D of which the main effective components are Streptomyces vinaceus-drappus and Gliocladium roseum. The multifunctional active organic fertilizer can passivate heavy metals in soil, activate the soil, improve the microecological environment of the rhizosphere, degrade the chemical pesticides and improve the crop quality. The multifunctional active organic fertilizer is used in the field of agricultural planting.

The invention discloses a nano silver antibacterial latex pillow or mattress and a preparation method thereof. Tiny air holes are fully distributed on the surface of the nano silver antibacterial latex pillow or mattress, and air is filled into the tiny air holes; and the silver antibacterial latex pillow or mattress has bacterial resistance on aspergillus niger, aspergillus flavus, discolored aspergillus, penicillium citrinum, paecilomyces varioti, herbarium cladosporium, trichoderma viride and chaetomium globasum. The preparation method comprises the following steps of: uniformly mixing nano sliver with natural latex; then respectively adding sulphur, bi(triethoxy propylsilane)tetrasulfide and 2,6-butylethylene-4-methylphenol; uniformly stirring; adding soap and air to generate foams; then adding gelatin; and compressing for moulding. The nano silver antibacterial latex pillow or mattress of the invention combines air permeability, high elasticity and soft touch feeling of the latex with efficient bacterial resistance and broad spectrum sterilizing performance of nano silver and can effectively overcome the defects of easy bacterial generation, difficult air permeation, and the like of a common pillow on the market.

The invention discloses Paecilomyces lilacinus FZ07-9F-5 CGMCC No.2780, microbial inoculum and application thereof. The microbial inoculum is wireworm killing biological microbial inoculum with slow application function, which is prepared by the following steps: using a macromolecular material, namely sodium alginate as basic skeleton, using mycelium and spore mixture of P. lilacinus FZ07-9F-5 CGMCC No.2780 as active components, using infusorial earth as a carrier, and adopting a method of sodium alginate-calcium chloride reaction according to the characteristic of forming gel by the sodium alginate and polyvalent cations to be prepared into the microbial inoculum. Proved by experiments, the microbial inoculum has better wireworm killing efficacy, has control ratio up to between 50 and 60 percent, is comparatively stable, has no noxious chemical residues (innocuous and non-residue), does not pollute agricultural products and environment (does not react with other substances in the environment), and has the characteristics of high cost performance and convenient application. The microbial inoculum plays an important role in control of wireworm diseases of plant roots and knots, and has wide application prospect.

The invention provides a method for increasing the yield of natural cordyceps sinensis. The method comprises the following steps of (1) selecting a base for increasing the yield of natural cordyceps sinensis; (2) placing a trapping lamp; (3) preparing trapping liquid; (4) screening and preparing culture medium of hepialidae hirsutella sinensis and paecilomyces hepialid; and (5) controlling yield increase conditions. According to the method, at the adult stage, a high pressure mercury vapor lamp is used for trapping, so the imagoes of hepialus armoricanus around the lamp fly to the base, and the egg-laying amount is increased; ethanol, vinegar and sugar solution are sprayed on meadows beneficial to larva growth, so the larvae gather together; and the hepialidae hirsutella sinensis and the liquefied paecilomyces hepialid are sprayed on the gathering area of larvae to raise the infection rate of the larvae of hepialus armoricanus, so the aim of increasing the yield of cordyceps sinensis is achieved and the industrialized production is realized.

The invention discloses paecilomyces lilacinus and a cultivating method thereof and applications thereof. The method comprises steps of: A. activation of paecilomyces lilacinus PL461, which comprises steps of: placing paecilomyces lilacinus PL461 bacterial strains on a PDA medium board, cultivating the paecilomyces lilacinus PL461 bacterial strains, and activating the paecilomyces lilacinus PL461 bacterial strains; B. liquid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: putting the activated paecilomyces lilacinus PL461 bacterial strains into an Erlenmeyer flask containing a liquid medium of PDB and Czapek's, cultivating the above bacterial strains with shaking, and taking the above cultivated bacterial strains as standby; C. solid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: mixing a solid fermentation cultivation medium uniformly according to a volume ratio, wherein the medium comprises dry compost rice bran, corn flour and water, putting the mixed medium into a domestic fungus culture bag, inoculating the paecilomyces lilacinus PL461 fermented bacteria solution into the solid fermentation cultivation medium employing culture dish fermentation after disinfection and cooling, and putting the above inoculated solid fermentation cultivation medium into a culture dish after stirring uniformly again to cultivate and take as standby; and D. preparation of a paecilomyces lilacinus bacteria agent from the solid phase culture according to a formula and the method. The paecilomyces lilacinus has an obvious lethal effect on nematode eggs, equipment is simple, production cost is reduced greatly, and the cultivating method is suitable for mass production of paecilomyces lilacinus.

The invention discloses a liquid fermentation culture method of Paecilomyces lilacinus, comprising: preparing Paecilomyces lilacinus spore suspension; preparing a liquid fermentation medium of Paecilomyces lilacinus; inoculating lavender in the liquid fermentation medium Paecilomyces spore suspension, fermentation culture; the composition of the liquid fermentation medium includes a carbon source and a nitrogen source; the carbon source is selected from any one of glucose, sucrose, maltose, soluble starch or corn flour or Various; the nitrogen source is selected from any one or more of sodium nitrate, ammonium sulfate, peptone or soybean flour. The present invention screens and compares the various process parameters of the fermentation culture of Paecilomyces lilacinus, including the composition and dosage of the culture medium, the initial pH value of the culture medium, the inoculum size of the spore suspension, the temperature and the rotating speed of the fermentation culture, etc. By optimizing, the most suitable process parameters for sporulation were obtained. The fermentation culture method of the invention has high spore yield and relatively low production cost, and is suitable for large-scale production of Paecilomyces lilacinus fungicide.

The present invention relates to a paecilomyces genus microorganism and microbial Mixing insecticide for controlling the soil pests using the same, more particularly to a paecilomyces genus microorganism having insecticidal activity to soil pests and a microbial insecticide for controlling the soil pests containing the same. Since the microbial insecticide of the present invention shows excellent insecticidal activity to harmful soil pests, it can effectively be used as a environment-compatible insecticide to prevent the soil pests from farmland crops.

The invention provides a selenium-rich bio-organic fertilizer. The selenium-rich bio-organic fertilizer is prepared from the following ingredients in parts by mass: 40-80 parts of organic residue, 0.01-0.1 part of seleniferous compound, 10-15 parts of NPK, 0.5-1 part of microbial agent and 5-10 parts of auxiliaries, wherein the microbial agent is prepared from bacillus licheniformis, paecilomyces lilacinus and bacillus amyloliquefaciens, the effective viable count ratio of the bacillus licheniformis to the paecilomyces lilacinus to the bacillus amyloliquefaciens is (1 to 100): (1 to 200): (10 to 100), and the effective viable count of the microbial agent is greater than 0.2 billion per gram. According to the selenium-rich bio-organic fertilizer, selenium and the paecilomyces lilacinus are in synergism in a reasonable proportioning ratio, and thus the nematode inhibiting effect is better; and the seleniferous compound, organic matters, the microbial agent and the auxiliaries are in synergism, so as to improve the quality and yield of crops.

The invention provides a functional bactericide for preventing and treating root-knot nematode.The functional bactericide comprises an azotobacter bactericide, a biocontrol bacterium bactericide and a carrier, and the mass ratio of the azotobacter bactericide to the biocontrol bacterium bactericide to the carrier is (1-2.5):(1.5-5):1.The azotobacter bacterium bactericide contains nodule bacteria and azotobacter chroococcum, and the living bacterium count ratio of nodule bacteria to azotobacter chroococcum is (1-100):(1-200).The biocontrol bacterium bactericide contains paecilomyces lilacinus, verticillium chamydosporium, bacillus subtilis and bacillus cereus, and the living bacterium count ratio of paecilomyces lilacinus to verticillium chamydosporium to bacillus subtilis to bacillus cereus is (1-100):(1-100):(1-100):(1-100).The living bacteria count of the functional bactericide is larger than 0.2 billion/g.When applied to fertilizer, the functional bactericide can improve the disease preventing effect and the growth promoting effect of microbial fertilizer, guarantee nutrition supply of microbial fertilizer in the applying process, achieve the effects of preventing and treating root-knot nematode, forming healthy root nodules and promoting plant growth, increase the yield of crops and improve the quality of crops.

The invention provides a disinsection fungi emulsifiable powder, comprising the following components: disinsection fungi spore powder or mycelium 40-60, vegetable oil 5-15, surfactant 5-10, stabilizer 5-10, dispersant 0.1-1; wherein the disinsection fungi is metarrhizium anisopliae, white muscardine fungi, paecilomyces, hirsutella or the mixture; the surfactant is nonionic surfactant, the vegetable oil is one kind or the mixture of many kinds of the oil according to arbitrary ratios selected from the palm oil, corn oil, soybean oil, cotton seed oil and colza oil; the stabilizer is one kind or the mixture of many kinds of the oil according to arbitrary ratios selected from the xanthan gum, carboxymethyl cellulose, gelatin; the dispersant is DW 1003, glucose ester of fatty acid or the mixture thereof. The storage and transportation of the disinsection fungi emulsifiable powder is convenient and the storage period of the fungi spore is effectively prolonged and the long-term storage is convenient and the preparation activity is higher; after application of the disinsection fungi emulsifiable powder, the oil can effectively absorb the ultraviolet ray and reduce the damage of the ultraviolet ray on the fungi spore, effectively increase the field control-effect stability. The process production technique of the disinsection fungi emulsifiable powder is simple and the disinsection fungi emulsifiable powder is easily mixed with the other compatible synergistic pesticide.

The invention discloses a paecilomyces lilacinus strain and its culture method and use. The paecilomyces lilacinus strain is paecilomyces lilacinus CPLI0708, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No.8450. The culture method of the paecilomyces lilacinus strain comprises the following steps of A, bacterial strain activation: carrying out bacterial strain streak cultivation activation on a PDA inclined plane, B, bacterial strain liquid fermentation culture: transferring the activated bacterial strain into a triangular flask with a liquid medium and carrying out shaking culture, and C, bacterial strain solid fermentation culture: mixing a carrier, a nutrition matrix and water according to a volume ratio to form a uniform mixture which is a solid fermentation medium, putting the solid fermentation medium into a fresh-keeping bag, inoculating the solid fermentation medium with a liquid fermentation seed, carrying out stirring to obtain a uniform mixture and carrying out culture. The bacterial strain conidiospore has strong effects of killing sucking-type insects such as greenhouse aphids and whitefly and the fermentation broth has strong effects of killing larvas of soybean cyst nematode and can be used for pollution-free vegetable production.

The invention discloses a compound microorganism bacterium agent for preventing and treating meloidogyne and a preparation method of the compound microorganism bacterium agent. The compound microorganism bacterium agent comprises the following ingredients: paecilomyceslilacinus (Thom) samson fermentation liquid and verticilliumchlamydosporium fermentation liquid. The field meloidogyne prevention and treatment test results show that after paecilomyceslilacinus (Thom) samson microbial inoculums and verticilliumchlamydosporium microbial inoculums are compounded, the paecilomyceslilacinus (Thom) samson microbial inoculums and the verticilliumchlamydosporium microbial inoculums show the very obvious cooperative synergia meloidogyne prevention and treatment effect through being compared with the single paecilomyceslilacinus (Thom) samson microbial inoculums or the verticilliumchlamydosporium microbial inoculums, and the prevention effect on the meloidogyne is obviously improved. In addition, the integral prevention effect of the compound microorganism bacterium agent on the meloidogyne is also more and more stable.

The invention relates to a paecilomyceslilacinus strain having strong pathogenicity for diaphorina citri and belongs to the technical field of the microorganism. The strain is paecilomyceslilacinus ZJPL08 which is registered and conserved in China General Microbiological Culture Collection Center on March 15, 2013, and the conservation number of the strain is CGMCC No. 7318. The paecilomyceslilacinus ZJPL08 provided by the invention has stronger pathogenicity for diaphorina citri and can be developed into a biocontrol agent for biological prevention and treatment of diaphorina citri; and compared with the present chemical agent for preventing and treating diaphorina citri, the paecilomyceslilacinus ZJPL08 has the characteristics of high efficiency, low toxicity, low residue, no pollution and not easy creation of resistance to drugs. The strain ZJPL08 has stronger acting effect on diaphorina citri than other paecilomyceslilacinus strains; the strain ZJPL08 is quick to cultivation, high in sporulation quantity, high in germination rate of spores and easy for preparation, and has excellent market application prospect.

The present invention relates to animal feed additives, which additives comprise a monocomponent xylanase derived from a strain of Byssochlamus, Chaetomium, Humicola, Malbranchea, Mucor, Myceliophthora, Paecilomyces, Talaromyces, Thermoascus, or Thielavia. In other aspects, the invention relates to monocomponent xylanase preparations, DNA constructs, recombinant expression vectors, host cells, and methods of producing monocomponent xylanase preparations.

The invention relates to a paecilomyces lilacinus-containing composition for preventing and controlling nematodes. The composition contains paecilomyces lilacinus and acephate in a weight ratio of (50-600):(1-7), wherein the living bacterium content of the paecilomyces lilacinus is between 1 and 10 billion colony-forming units (cfu)/gram. The composition can prevent and control the nematodes effectively and exert a synergistic effect on the prevention and control over the nematodes. In addition, the acephate also can kill the nematodes, so pests in growth environment of crops also can be killed while an effect of killing the nematodes by the paecilomyces lilacinus is enhanced; and an effect of preventing and controlling the nematodes and other pests simultaneously by applying pesticides at one time is achieved.

The invention discloses a paecilomyces lilacinus space mutation mutant strain Sd-m-16 and a microbial preparation and an application thereof. A paecilomyces lilacinus strain 20-7 and a Shandong strain are carried on the Shenzhou 8 spacecraft for space mutation, and a non-carried original strain is used as a contrast to compare the variability of the space-carried strain on aspects of configuration, pigment change, growth speed, sporulation quantity, pathogenicity and the like to screen a mutant strain with improved growth performance and greatly improved pathogenicity on root-knot nematode eggs. The screening on the growth speed, sporulation quantity and pathogenicity of the space-carried strain indicates that wide positive mutation has been generated on such aspects of the space mutant strain Sd-m-16 of the Shandong strain as growth property, pathogenicity to root-knot nematode eggs and the like; particularly, the pathogenicity of the mutant strain is greatly improved in comparison with that of the original strain. The invention further provides a microbial preparation containing the space mutant strain and an application of the space mutant strain in biological prevention and cure of root-knot nematodes.

The invention discloses a bacterial strain separated from tomato root soil in Minhou county of Fuzhou City in Fujian province. The Paecilomyces lilacinus bacterial strain FJAT-9041 is preserved in China General Microbiological Culture Collection Center (CGMCC) on 14th, may 2012, and has a preservation number of CGMCC NO.6110. The invention discloses a method for preparing a bactericide for degrading phoxim by using the Paecilomyces lilacinus bacterial strain FJAT-9041. The invention has the advantages that the Paecilomyces lilacinus bacterial strain FJAT-9041 is provided, and the bactericide for degrading the phoxim is prepared by using the Paecilomyces lilacinus bacterial strain FJAT-9041, and the obtained bactericide has an efficient degradation effect on phoxim.

The invention discloses a method for producing a green feed additive by potale for beast and bird healthy cultivation. The method comprises the steps of potale mixing subpackage, medium sterilization, liquid strain making, inoculation fermentation, and drying and crushing. The method adopts chlorine dioxide to sterilize a potale culture medium, and Paecilomyces varioti fungus to ferment the potale. In the additive produced by the method, protein content is improved to 26.8 to 30.5 percent from 17 percent, energy value is improved to 4,250kCal/kg from the previous 2,510kCal/kg, and direct available digestibility is improved to 62 percent from 33 percent. Main compositions contain more than 10 kinds of nucleotide substances, mannite, fungal polysaccharide, superoxide dismutase, amino acid, sterol, alkaloid, VE, VB1, VB2, mineral substances and the like. Animal experiments prove that the additive has wide action and efficacy. The method has the characteristics of wide raw material source, easy production, controllable quality, low cost, wide application and the like, is applicable to large-scale industrial production, and provides a new approach for high-value utilization of wine industrial residue.

The invention discloses a preparation used for strengthening immunity and inhibiting tumor. The preparation is characterized by being prepared from following raw materials by weight proportion: 290-350g bat moth paecilomyces powder, 72-84g medlar extract, and 1-5g taurine; and the preparation can be prepared by the steps of: after processes of enzymolysis extraction and the like, superfine grinding and mixing to obtain fine powder, and combining with pharmaceutical excipients to make into capsules, tablets, granules and the like. The invention has the advantages that, through all actions of the bat moth paecilomyces powder, the taurine and the medlar extract, immune cells are activated and immunity function of all organic tissues and systems is enhanced, thereby achieving the effects of improving organism immunity function and enhancing resistibility; and meanwhile, the preparation can inhibit tumor growth and kill tumor cells and has a favorable anti-tumor effect. The invention is suitable for curing various infections caused by week immunity and other diseases and for convalescents after tumor operations.