38 results about "Paecilomyces" patented technology

The invention discloses a special fertilizer composition for high-yield cultivation of sugarcane, and belongs to the technical field of sugarcane planting. Comprising a disease-resistant fertilizer and an insect-resistant fertilizer; wherein the disease-resistant fertilizer comprises the following raw materials in parts by mass: 50 parts of diatomite, 5-10 parts of biomass charcoal, 1-2 parts of a compound microbial agent, 150-170 parts of an organic fertilizer and 50-60 parts of an inorganic fertilizer; wherein the compound microorganism bacterium agent comprises a bacillus subtilis bacterium agent, a trichoderma harzianum bacterium agent, a trichoderma viride bacterium agent and a paecilomyces lilacinus bacterium agent; the insect prevention fertilizer comprises beauveria bassiana suspension and matrine in a volume ratio of (3.5-4.5): 1. According to the fertilizer composition special for high-yield cultivation of sugarcane, the synergistic effect of microorganisms is utilized, the broad-spectrum inhibition effect on microorganisms in sugarcane continuous cropping disease soil can be achieved, the kieselguhr and the biomass charcoal cooperate to form a porous substance, nutrient elements are adsorbed in pores, the slow-release effect is improved, the fertilizer efficiency is prolonged, and the effect is good. Moreover, the soil structure can be improved, and the disease resistance of the sugarcane during continuous cropping is greatly improved through the synergistic interaction with the microbial complex microbial inoculants.

The invention relates to the technical field of microbial agents. The invention provides a microbial agent for inhibiting root-knot nematode and a preparation method thereof. The microbial agent is prepared from the following raw materials in parts by weight: 6-10 parts of paecilomyces lilacinus liquid, 5-7 parts of bacillus thuringiensis liquid, 6-8 parts of beauveria bassiana culture, 6-10 parts of bacillus subtilis liquid and 50-60 parts of a carrier. The microbial agent disclosed by the invention can effectively prevent and treat root-knot nematodes, reduce the influence of the root-knot nematodes on crops, and improve the soil fertility at the same time. The effects of biologically controlling insects, biologically fertilizing soil and promoting plant growth are achieved, and the yield and quality of crops are improved.

The invention discloses bacillus and application thereof, and belongs to the technical field of microorganisms, the bacillus is preserved in China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20242290, and the preservation date is October 22, 2024. The composition can effectively inhibit vegetative growth and vegetative propagation of pathogenic bacteria such as colletotrichum gloeosporioides and paecilomyces japonicus, and has a good and broad-spectrum control effect on various pathogenic fungi. Bacteriostatic components contained in fermentation filtrate of the bacillus can remarkably inhibit germination of colletotrichum spruce spores and formation of appressorium. And the bacillus has a good prevention and treatment effect on the citrus huanglongbing.

The invention discloses paecilomyces hepialid powder and a production process thereof, and belongs to the technical field of microorganisms. The production process comprises the following steps: S01, slant strain preparation; s02, shaking the seed solution in a flask; s03, fermenting in a fermentation tank; s04, performing centrifugal separation; s05, drying is carried out; and S06, crushing and sieving to obtain the paecilomyces hepialid powder. According to the paecilomyces hepialid powder obtained by the invention, the yield is 42 to 55 g / L, and the adenosine content is 13.29 to 14.83 g / kg; the content of cordycepin is 2.14 to 3.08 g / kg. Static-high-temperature fermentation is introduced in the fermentation process, the obtained paecilomyces hepialid powder has the characteristics of high biomass, high adenosine content and high cordycepin content, the problems of scarcity of cordyceps sinensis resources and high medicine price can be solved, the contradiction between supply and demand can be relieved, the environment can be protected, the cost of the cordyceps sinensis preparation can be reduced, and the application prospect is wide. And the effect of a medication group of the cordyceps sinensis preparation as a tonic drug is increased.

This invention relates to the field of fertilizer technology, specifically to a disease-resistant microbial compound fertilizer and its preparation method. The disease-resistant microbial compound fertilizer comprises the following raw materials in parts by weight: 10-15 parts nitrogen fertilizer, 10-20 parts phosphorus fertilizer, 6-10 parts potassium fertilizer, 50-70 parts fermented organic matter, 8-12 parts fertilizer efficiency enhancer, 5-8 parts disease-resistant composition, and 6-10 parts chitosan oligosaccharide. The disease-resistant composition is a mixture of sodium selenite and potassium humate in a weight ratio of 1:1.5-2.5. The microbial compound fertilizer prepared by this invention not only achieves a combination of rapid and long-lasting effects, improving fertilizer utilization, but also increases organic matter, activates fixed phosphorus and potassium elements in the soil, and improves the micro-ecological environment. Furthermore, through the synergistic effect of Trichoderma harzianum inoculum, Paecilomyces lilacinus, the disease-resistant composition, and chitosan oligosaccharide, it exhibits good inhibitory effects on soil-borne diseases and some foliar diseases, promoting robust crop growth and improving both crop yield and quality.

The present invention discloses a microbial inoculant for preventing both root-knot nematode disease and clubroot disease and a preparation method thereof, relating to the technical field of fertilizers, and solving the technical problems in the prior art that the field symptoms of root-knot nematode disease and clubroot disease are similar and difficult to quickly distinguish and accurately select agents, and that root-knot nematode disease and clubroot disease are likely to occur simultaneously and difficult to control in a timely manner. The preparation method of the microbial inoculant for preventing both root-knot nematode disease and clubroot disease of the present invention comprises the following steps: preparing methylotrophic bacillus powder and bacillus subtilis powder; preparing paecilomyces lilacinus powder; and compounding the prepared methylotrophic bacillus powder, paecilomyces lilacinus powder and bacillus subtilis powder in proportion and mixing them evenly to obtain the microbial inoculant for preventing both root-knot nematode disease and clubroot disease. The microbial inoculant for preventing both root-knot nematode disease and clubroot disease prepared by the present invention can conduct unified prevention and control of the two diseases, avoiding the golden period of early prevention and control being delayed due to incorrect agent selection in agricultural production.

The application provides a composite microbial agent, application of the composite microbial agent and a treatment method of high-concentration organic wastewater. The active component of the composite microbial agent provided in the first aspect of the application comprises two or three of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain. The composite microbial agent provided in the first aspect of the application can be used for biological decontamination treatment of high-concentration organic wastewater, so as to realize degradation of organic pollutants in the high-concentration organic wastewater and pH value adjustment, thereby avoiding increase of the load of a municipal sewage treatment system.

The present invention relates to a liquid carrier concentrate comprising at least one beneficial microorganism selected from the group consisting of Bacillus, Streptomyces, Beauveria, Trichoderma, Metarhizium, Paecilomyces, Isaria, Baculovirus, Azospirillum, Penicillium and Rhizobium and at least one dioxolane-based solvent. The present invention also relates to an agricultural active formulation obtainable by diluting or dispersing said liquid carrier concentrate. The invention also concerns a method for treating soils, plants and / or seeds to control pests and / or to regulate the growth of plants and / or to induce the defense response in plants and / or to enhance metabolic and physiological processes within plants and soils, by applying the liquid carrier concentrate or the agricultural active formulation.

The invention relates to the technical field of ecological agriculture, in particular to a method for fermenting organic fertilizer liquid by using oil residue as a water fertilizer, which comprises the following steps: pouring organic substances into a fermentation tank, adding pond water for soaking, stirring at regular time, adding slag melting bacteria liquid, and stirring at regular time; adding pond water, brown sugar and lactobacillus into the sealed tank, uniformly stirring, activating propagation, and injecting the organic matter soaking solution into the sealed tank for fermentation; the method comprises the following steps: preparing a fermentation tank, injecting pond water, respectively adding EM bacteria, BFA bacteria, straw reactor bacteria, bacillus licheniformis, streptomyces microflavus, paecilomyces lilacinus and trichoderma harzianum, then adding brown sugar and an activating microbial agent for later use, and stirring at regular time; extracting a fermentation product, filling a fermentation tank with the fermentation product, adding mineral substances to form an organic matter ion-state molecular group, adding activated lactic acid bacteria liquid and brown sugar, and continuing fermentation; other medium trace element fertilizers are added into the zymophyte liquid, uniform stirring is performed, drip irrigation is performed for use, the organic fertilizer liquid fermented through the method can rapidly and massively propagate in the soil, and other organic matter in the soil can be further utilized.

The invention discloses a bacillus wurtzeri strain and application thereof, and belongs to the technical field of microorganisms. The bacillus wurtzeri strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20242289, the preservation date is October 22, 2024, and the preservation address is Wuhan University, Wuhan, China. The bacillus wurtzeri strain disclosed by the invention can effectively inhibit vegetative growth and vegetative propagation of pathogenic bacteria such as colletotrichum gloeosporioides and paecilomyces radinieri, has a remarkable inhibition effect on citrus liberobacter asiaticum and has a good prevention and treatment effect on the citrus liberobacter asiaticum.

This invention relates to the field of *Penicillium cicadae* technology, specifically to a high-efficiency fermentation process for *Penicillium cicadae* based on a composite culture medium, comprising the following steps: inoculating *Penicillium cicadae* strain into a composite culture medium; subsequently controlling temperature and aeration according to the fermentation stage; adding a metabolism promoter after 72 hours of fermentation; and obtaining the fermentation broth and separating *Penicillium cicadae* after fermentation. This invention rapidly delivers nutrients in a liquid phase to promote rapid mycelial proliferation, while utilizing the porous structure of the solid phase to enhance the adsorption and expansion capacity of the mycelium, thereby increasing mycelial density and the synthesis efficiency of metabolically active substances. A precise temperature control strategy matches the physiological transition from mycelial growth to product accumulation, ensuring enzyme activity and reducing heat damage. The addition of a metabolism promoter synergistically stimulates the efficient expression of target metabolites, improving the yield and quality of the target product.

The invention relates to the technical field of planting, in particular to a fungicide and fertilizer suitable for planting landscape plants. The microbial inoculum disclosed by the invention is prepared from the following components in parts by mass: 5 to 10 parts of bacillus coagulans microbial inoculum, 4 to 8 parts of bacillus laterosporus microbial inoculum, 10 to 18 parts of bacillus mucilaginosus microbial inoculum, 6 to 10 parts of bacillus velezensis microbial inoculum, 2 to 6 parts of trichoderma viride, 6 to 10 parts of bacillus amyloliquefaciens microbial inoculum and 2 to 6 parts of paecilomyces lilacinus. Through compatibility of various microbial agents, the soil structure can be improved, and the soil fertility can be improved. The microbial agent disclosed by the invention is prepared into a fertilizer, and the obtained fertilizer also has the effects of regulating the soil microhabitat, improving the soil structure and improving the soil fertility. The growth of garden plant roots can be promoted, and the growth performance of garden plants is improved. And a basis is provided for planting of landscape plants.

The invention provides an application of paecilomyces cicadae in prevention and treatment of phenacoccus solenopsis and a fungicide. The corrected fatality rate of a spore suspension of the paecilomyces cicadae independently used on the phenacoccus solenopsis is 64.04-76.40%, and the influence of the phenacoccus solenopsis on crops is effectively prevented and treated; after the paecilomyces cicadae is combined with a pesticide, the paecilomyces cicadae has synergistic, additive or antagonistic effects on prevention and treatment of the phenacoccus solenopsis, and can improve the insecticidal ability of insecticidal fungi, reduce the dosage of the pesticide, realize the effects of biological control and plant growth promotion, and improve the yield and quality of crops.

The invention discloses paecilomyces lilacinus and application thereof, and relates to the technical field of microorganisms, and the technical key points are as follows: the classification name of the paecilomyces lilacinus is Purpureobacterium lilacinus, the paecilomyces lilacinus is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 16, 2025, the preservation number is CGMCC No.41756, and the preservation number is CGMCC No.41756. The paecilomyces lilacinus can be applied to prevention and control of pine wood nematodes. According to the method, the pine wood nematodes are controlled through microorganisms, the death rate of the pine wood nematodes is high, the insect killing time is short, and compared with a traditional pine wood nematode killing method, the method is high in insect killing efficiency and low in pollution.

The invention discloses a pepper phytophthora blight prevention and control method, which belongs to the technical field of biology, and comprises the following steps: when pepper seedlings are transplanted and when a first pepper phytophthora blight plant appears, a compound microbial agent is used for irrigating roots of pepper plants; the volume ratio of the paecilomyces lilacinus suspension to the bacillus mojavensis suspension to the penicillium expansum suspension is (3-5): (1-2): 1, the effective viable count of the paecilomyces lilacinus suspension is 2 * 10 < 8 > CFU / mL, and the effective viable count of the bacillus mojavensis suspension and the effective viable count of the penicillium expansum suspension are both 1 * 10 < 8 > CFU / mL. The prevention and treatment method can effectively prevent and treat pepper phytophthora blight.

The invention belongs to the technical field of biopesticides, and discloses an Akanthomyces varioti strain biocontrol agent and application thereof in crop nematode disease prevention and control, the preservation number of the Akanthomyces varioti strain in China Center for Type Culture Collection is CGMCC No: 41597, the biocontrol agent is fermentation liquor containing the Akanthomyces varioti strain, the preservation number of the biocontrol agent in China Center for Type Culture Collection is CGMCC No: 41597, and the biocontrol agent can be applied to crop nematode disease prevention and control. The bactericidal composition can be applied to prevention and treatment of crop nematode diseases. The echinococcus caerulea biocontrol agent has a strong lethal effect on second-stage larvae (J2) of meloidogyne incognita and inhibits infection of the meloidogyne incognita J2 on plants. The biocontrol agent has the characteristics of high efficiency and low toxicity, can reduce the generation of nematode resistance to drugs, is beneficial to environmental protection, can reduce pesticide residues on agricultural products, and has a wide application prospect.

The present invention relates to a method for separating and purifying cordycepin from the mycelia of Paecilomyces elegans, comprising: (1) crushing and sieving the dried mycelia of Paecilomyces elegans to obtain mycelia powder of Paecilomyces elegans; (2) subjecting the mycelia powder of Paecilomyces elegans to a composite enzymatic hydrolysis treatment with cellulase and protease, filtering, and collecting the filtrate; (3) passing the filtrate through a macroporous resin column, eluting with water and an ethanol aqueous solution in sequence, and collecting the eluate; (4) purifying the eluate by high performance liquid chromatography to obtain the cordycepin. The method for separating and purifying cordycepin involved in the present invention does not involve the use of heat extraction and toxic solvents, and does not involve strong acid or strong base substances in the whole process. It has the advantages of short extraction time, mild reaction conditions, non-denaturation of the product, and high product purity, and is a low-carbon and green chemical preparation and separation process.

The application discloses a method for enhancing the sensitivity of Paecilomyces fungi to ultraviolet radiation, and belongs to the technical field of biology. Paecilomyces Based on the discovery that the biosynthesis of the tough core component pyridine dicarboxylic acid (DPA) of Paecilomyces fungi spores depends on iron-mediated enzyme activity, free iron ions are effectively stored and DPA synthesis is inhibited by applying a specific concentration of iron chelator, so that the sensitivity of Paecilomyces fungi spores to ultraviolet (UV-A and UV-B) radiation is significantly enhanced. Paecilomyces The application provides a low-cost, efficient and environment-friendly fungal control strategy, and has important application value in the fields of food safety, agriculture and industrial sterilization.

The invention relates to the field of agriculture, in particular to a saline-alkali soil conditioner and a preparation method thereof. The saline-alkali soil conditioner is prepared from the following components in parts by weight: 20 to 40 parts of decomposed animal manure, 1 to 5 parts of boric acid, 30 to 40 parts of desulfurized gypsum and 20 to 30 parts of potassium fulvate; wherein the decomposed animal waste contains 3-5 parts of biological enzyme and 4-6 parts of compound microorganisms; in the compound microorganism, the concentration ratio of paecilomyces lilacinus to bacillus subtilis to bacillus megatherium is (1-2): (1-2): (1-2). On the premise of reducing the dosage of the desulfurized gypsum, the soil acidity and alkalinity are regulated by utilizing the synergistic effect of the compositions, and the physicochemical properties of the saline-alkali soil are improved, the salinization degree of the soil is reduced, the aggregate and porosity of the soil are increased, and the salt elimination and stain reduction capabilities of the soil are improved through soil biochemical reaction; meanwhile, the aggregate stability of the soil is improved, the water storage capacity of the soil is improved, and the soil surface salt-alkali content is reduced.

The invention discloses a compound microorganism bactericide and a preparation method thereof, and relates to the technical field of agricultural microorganism application. Comprising the following components: 10 to 15 parts of a core strain, 3 to 5 parts of humic acid, 4 to 6 parts of plant polysaccharide, 8 to 12 parts of a protective agent, 2 to 4 parts of a nutritional ingredient, 1 to 3 parts of adenosine, 2 to 4 parts of nano-scale hydroxyapatite, 1 to 2 parts of graphene quantum dots, 0.8 to 1.5 parts of glutamine and 1 to 3 parts of carboxymethyl-beta-cyclodextrin. The core strains comprise paecilomyces lilacinus, bacillus amyloliquefaciens, trichoderma harzianum, trichoderma viride, bacillus subtilis, pseudomonas fluorescens, bacillus licheniformis, brevibacillus laterosporus and candida utilis. The bactericide is remarkable in sterilizing effect, sufficient in stability and good in field control effect.

The invention discloses a Paecilomyces lilacinus, a bacterial agent and a preparation method thereof, wherein the Paecilomyces lilacinus PM0327-3 has a preservation number of GDMCC No: 62375. The Paecilomyces lilacinus PM0327-3 has the ability of resistance to metabolite antagonism, high temperature resistance, acid and alkali resistance, and has good application prospects.

The invention provides a preparation method of a penicillium krusei agent composition for improving saline-alkali soil, and relates to the technical field of soil improvement, and the preparation method specifically comprises the following steps: respectively inoculating bacillus polymyxa and penicillium krusei to an NA solid culture medium and a PDA solid culture medium, and diluting with purified water to obtain a bacterial suspension; mixing the obtained bacterial suspensions, and adding an artemisia sacrorum extract into the obtained mixture to obtain a bacterial agent composition; wherein the name of the penicillium kenii strain is penicillium kenii 23301-20, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41049, and the preservation unit is China General Microbiological Culture Collection Center. The microbial agent composition prepared by the method provided by the invention can improve the planting conditions of the saline-alkali soil for a long time.

The invention discloses 2, 6-dipicolinic acid synthase PmDpa and application thereof, and the amino acid sequence of the 2, 6-dipicolinic acid synthase PmDpa is shown as SEQ ID NO. 1. The invention also discloses a preparation method of the 2, 6-dipicolinic acid synthase PmDpa. The invention discloses the gene sequence of the DPA synthase PmDpa in paecilomyces megaterium for the first time, designs excavation, cloning, expression and purification of the DPA synthase in a paecilomyces megaterium genome to obtain the DPA synthase PmDpa, and further verifies the application of the DPA synthase PmDpa in catalytic synthesis of 2, 6-dipicolinic acid.

The invention relates to the technical field of pest control, and particularly discloses a physical and chemical combined pesticide application method for guava nematode control. The invention discloses a physical and chemical combined pesticide application method for guava nematode prevention and control. The method comprises the following steps: S1, soil environment physical regulation and control; s2, performing high-temperature smoldering pretreatment; s3, chemical agent root irrigation: in the fruit bearing period of the guavas or the initial stage of nematode damage development, the nematicide composition is diluted with water and then uniformly sprayed to soil near a crown water drip; s4, synergistic application of a biological agent: applying a paecilomyces lilacinus preparation with the application amount of 1 kg / mu before and after root irrigation treatment by using a chemical agent, and adding 1000 liters of water to spray roots or apply the paecilomyces lilacinus preparation in furrows; s5, implementation of a prevention and treatment period. The combined pesticide application method can be used for comprehensive prevention and control of nematodes in the psidium guajava planting process, and is suitable for continuous cropping plots with serious nematode harm or production parks pursuing green sustainable prevention and control; the method has the advantages of strong prevention and treatment periodicity and multiple action mechanisms.

The invention relates to the technical field of microorganisms, in particular to application and a method for degrading aflatoxin, in particular to application of a hirsutella sinensis strain and paecilomyces hepialid in degrading aflatoxin, and the hirsutella sinensis and the paecilomyces hepialid can effectively degrade various aflatoxin in a short time. The strain has a very good application value in the fields requiring degradation of aflatoxin, especially in the fields of medicines, foods and feeds.

The present invention relates to a liquid carrier concentrate comprising at least one beneficial microorganism selected from the group consisting of Bacillus, Streptomyces, Beauveria, Trichoderma, Metarhizium, Paecilomyces, Isaria, Baculovirus, Azospirillum, Penicillium and Rhizobium and at least one specific solvent. The present invention also relates to an agricultural active formulation obtainable by diluting or dispersing said liquid carrier concentrate. The invention also concerns a method for treating soils, plants and / or seeds to control pests and / or to regulate the growth of plants and / or to induce the defense response in plants and / or to enhance metabolic and physiological processes within plants and soils, by applying the liquid carrier concentrate or the agricultural active formulation.

The invention discloses application of paecilomyces hepialid in preparation of radix notoginseng rare saponins, and belongs to the technical field of biotransformation, the paecilomyces hepialid has the capability of efficiently converting radix notoginseng total saponins into various rare ginsenosides, and is a food-grade safe strain; the biological conversion method provided by the invention is mild in reaction condition, rich in conversion product, green and environment-friendly, not only improves the utilization rate of effective components in pseudo-ginseng, but also ensures the safety of the conversion product, and the conversion product has various pharmacological activities and has a good commercial application prospect.