Application and method for degrading aflatoxin

Abstract

The invention relates to the technical field of microorganisms, in particular to application and a method for degrading aflatoxin, in particular to application of a hirsutella sinensis strain and paecilomyces hepialid in degrading aflatoxin, and the hirsutella sinensis and the paecilomyces hepialid can effectively degrade various aflatoxin in a short time. The strain has a very good application value in the fields requiring degradation of aflatoxin, especially in the fields of medicines, foods and feeds.

Description

[0001] This application is a divisional application of the Chinese patent application with application number CN202110376570.0, application date April 7, 2021, and invention name “An application and method for degrading aflatoxin”. Technical Field

[0002] The present invention relates to the field of microbial technology, and in particular to an application and method for degrading aflatoxin, in particular to the application and method of Hirsutella sinensis and Paecilomyces hepiali in the degradation of aflatoxin. Background Art

[0003] Aflatoxins were first discovered in Aspergillus flavus isolated by British scientists in 1960. Aflatoxins can be mainly divided into two categories: blue fluorescent B and green fluorescent G. B includes B1, B2, B2α, G includes G1, G2; and also includes some derivatives such as aflatoxin M1, M2, P1, Q, H1, GM, toxic alcohol, etc. Aflatoxins are secondary metabolites produced by Aspergillus flavus or Aspergillus parasiticus. There are 18 types reported, but the most studied are aflatoxin B1, B2, G1 and G2. Among them, aflatoxin B1 is the most toxic and is identified as a Class I human carcinogen by the World Health Organization's Cancer Research Center. Its toxicity is 68, 75 and 10 times that of arsenic, ammonium nitrite and potassium cyanide, respectively.

[0004] Aflatoxin is seriously polluted in food and feed, posing a great threat to the health of humans and livestock, and traditional aflatoxin removal is mainly physical and chemical methods. The physical method mainly includes high temperature method, radiation method and physical adsorption method, and the chemical method includes alkali treatment method, oxidation method, etc., but these methods are prone to secondary pollution to the environment, reduce the nutritional value of the sample, and the toxicity of the degradation product is uncertain. The biological method has the characteristics of safety, high efficiency, irreversibility and environmental friendliness, and has become a hot research topic in the degradation of aflatoxin. At present, it has been reported that some microorganisms have the ability to remove aflatoxin, for example, Guan, etc., screening and identification of aflatoxin B1 efficient degradation strains and their degradation (Journal of Huazhong Agricultural University, Vol. 25, No. 2, March 2016). In the patent with publication number CN101766273B, a mushroom polysaccharide composition that can remove aflatoxin is disclosed, but the mushroom polysaccharide composition can only adsorb aflatoxin and cannot degrade it.

[0005] However, how to use known bacteria to effectively degrade aflatoxin still needs further research. Summary of the invention

[0006] The invention provides an application of Hirsutella sinensis and / or Paecilomyces pyralis in degrading aflatoxin.

[0007] Specifically, the above-mentioned aflatoxin can be any type of aflatoxin (AF), for example, B1, B2, B2α, G1, G2, or any derivative thereof, for example, M1, M2, P1, Q, H1, GM, toxin alcohol, etc., preferably AFB1, AFB2, AFG1, AFG2, and particularly preferably AFB1.

[0008] Specifically, the above-mentioned Chinese Hirsutum can be any Chinese Hirsutum strain. For example, different strains are obtained according to the prior art CN109082382A and CN105670937A, and are numbered as strain 1#, strain 2#, strain 3#, strain 4#, strain 5#, strain 6#, strain 7#, strain 8#, and strain 9#. The obtained sequence is subjected to similarity analysis with the data stored in GenBank (DNA sequence database), and according to the principles of microbial molecular genetics identification, it is identified as Chinese Hirsutum based on the homology of 18S rDNA sequence being higher than 95%.

[0009] Specifically, the above-mentioned Paecilomyces pygii can be any Paecilomyces pygii strain, such as CGMCC 3.15540, 3.17720, and 3.15421.

[0010] In one embodiment of the present invention, the above application is the application of Hirsutella sinensis in degrading Class B aflatoxins (such as AFB1, AFB2, AFB2α) and Class G aflatoxins (such as AFG1, AFG2).

[0011] In another embodiment of the present invention, the above application is the application of Paecilomyces avicularis in degrading Class B aflatoxins (such as AFB1, AFB2, AFB2α).

[0012] The invention also provides an application of the biological composition of Hirsutella sinensis in the degradation of aflatoxin.

[0013] The invention also provides an application of the biological composition of Paecilomyces avicularis in the degradation of aflatoxin.

[0014] The invention also provides an application of a biological composition of Hirsutella sinica and Paecilomyces avicularis in the degradation of aflatoxin.

[0015] Specifically, the biological composition is a composition related to the above-mentioned strain, which contains the bacterial bodies of the above-mentioned strain, such as live bacteria or cell fragments, intracellular substances or extracellular metabolites of the above-mentioned strain, one or more of the seed liquid, fermentation liquid, bacterial suspension, intracellular liquid, cell fragment liquid, seed liquid extract, fermentation liquid extract, and bacterial powder of the above-mentioned strain, especially one or more of the seed liquid, fermentation liquid, and bacterial suspension.

[0016] Specifically, the biological composition can be in any suitable form such as liquid, gel, powder, spray, etc., preferably in liquid form.

[0017] Specifically, the seed solution can be obtained by culturing the strain.

[0018] Specifically, the fermentation liquid is obtained by further culturing the seed liquid.

[0019] Specifically, the above-mentioned culture method includes: culturing the bacteria in an appropriate culture medium (especially liquid culture medium).

[0020] Specifically, the above-mentioned culture temperature can be any suitable culture temperature. For example, for the Chinese Hirsutella strain, the culture temperature can be 4-25°C, particularly 10-20°C, 13-16°C, for example 14°C, 15°C; for the bat moth Paecilomyces strain, the culture temperature can be 15-37°C, particularly 20-30°C, 24-26°C, for example 25°C.

[0021] Specifically, the culture may be a shaking culture, and the shaking frequency may be 80-200 rpm, particularly 100-150 rpm, for example, 100, 110, 120, 130, 140, 150 rpm.

[0022] Specifically, the above-mentioned culture time can be any suitable culture time. For example, for the Chinese Hirsutella spp. strain, the culture time can be 5-20 days, especially 10-15 days; for the Paecilomyces pyrifolia strain, the culture time can be 2-10 days, especially 4-6 days.

[0023] In one embodiment of the present invention, the above-mentioned culture medium may be PDB culture medium.

[0024] Specifically, the above-mentioned culture method also includes secondary culture.

[0025] Specifically, the secondary culture comprises: inoculating the culture solution into an appropriate culture medium for culture.

[0026] Specifically, the inoculation amount in the above-mentioned secondary culture is 1-20% (v / v), especially 5-10%.

[0027] Specifically, in the above-mentioned secondary culture, the culture temperature can be any suitable culture temperature. For example, for the Chinese Hirsutosporium strain, the culture temperature can be 4-25°C, especially 10-20°C, 13-16°C, for example 14°C, 15°C; for the bat moth Paecilomyces strain, the culture temperature can be 15-37°C, especially 20-30°C, 24-26°C, for example 25°C.

[0028] Specifically, in the above-mentioned secondary culture, the culture may be a shaking culture, and the shaking frequency may be 80-200 rpm, particularly 100-150 rpm.

[0029] Specifically, the above-mentioned culture time can be any suitable culture time. For example, for the Chinese Hirsutella spp. strain, the culture time can be 1-10 days, especially 4-6 days; for the Paecilomyces pyrifolia strain, the culture time can be 1-10 days, especially 1-3 days.

[0030] Specifically, in the above-mentioned secondary culture, the above-mentioned culture medium can be PDB culture medium.

[0031] In one embodiment of the present invention, the method comprises: inoculating the bacterial strain into PDB medium, culturing with shaking, and then transferring to PDB medium and culturing under the same conditions.

[0032] Specifically, the seed solution may be the secondary culture solution of the strain (as described above).

[0033] Specifically, the bacterial suspension can be prepared by suspending the bacterial cells of the above strain in a buffer solution.

[0034] In one embodiment of the present invention, the bacterial suspension can be prepared by suspending the bacterial cells obtained by separating the seed solution (for example, by centrifugation) in a buffer solution.

[0035] In one embodiment of the present invention, the buffer solution is PBS buffer solution.

[0036] Specifically, the intracellular fluid can be obtained as follows: the bacterial cells of the above strains are broken (for example, by grinding), and the resulting supernatant is the intracellular fluid.

[0037] Specifically, the cell debris solution can be prepared by breaking the bacterial cells of the strain (for example, by grinding), separating the cells, and suspending the obtained solid in a buffer solution.

[0038] In one embodiment of the present invention, the buffer solution is PBS buffer solution.

[0039] Specifically, the seed liquid extract and fermentation liquid extract can be obtained by appropriately extracting the seed liquid or fermentation liquid.

[0040] Specifically, the bacterial powder is a strain powder, especially a live bacterial powder, such as a live bacterial freeze-dried powder.

[0041] The present invention also provides an application of Hirsutella sinensis and / or Paecilomyces spp. in medicine, food and feed.

[0042] The invention also provides an application of the biological composition of Hirsutella sinensis in medicine, food and feed.

[0043] The invention also provides application of the biological composition of Paecilomyces avicularis in medicine, food and feed.

[0044] The invention also provides an application of a biological composition of Hirsutella sinica and Paecilomyces avicularis in medicine, food and feed.

[0045] Specifically, Hirsutella sinensis or Paecilomyces pyogenes, and biological compositions thereof have the above corresponding definitions of the present invention.

[0046] Specifically, the above application can be the application of Hirsutella sinensis and / or Paecilomyces spp. in the production, processing, storage, transportation and the like of drugs, food and feed.

[0047] Specifically, the above-mentioned medicines, foods and feeds can be any medicines, foods and feeds that may be contaminated by aflatoxin, which may be suspected of containing aflatoxin and / or having an unknown level of aflatoxin. Specifically, the above-mentioned application is an application of degrading (or even completely eliminating) aflatoxin in medicines, foods and feeds.

[0048] Specifically, the above-mentioned drugs can be Chinese medicinal materials (which themselves may be contaminated with aflatoxin) and Chinese medicinal products prepared therefrom, or drugs produced by microbial fermentation (wherein the microbial fermentation raw materials may be contaminated with aflatoxin, causing the produced drugs to be contaminated with aflatoxin).

[0049] Specifically, the above-mentioned food may be food consumed by humans on a daily basis, for example, may include grains, nuts and their products.

[0050] Specifically, the above-mentioned food can be cereals, for example, one or more of corn, wheat, barley, rye, rice, sorghum and millet; beans, for example, one or more of soybeans, peas and peanuts; oilseeds, for example, rapeseed, soybeans, sunflowers and cotton. The above-mentioned food can also be ground, for example, wet-milled or dry-milled grains, including milled fractions containing gluten / gluten, protein, starch, bran and / or oil.

[0051] Specifically, the above-mentioned nuts can be peanuts, walnuts, melon seeds, etc.

[0052] Specifically, the above products can be dairy products, seasonings (fermented seasonings such as fermented bean curd, fermented black beans, soy sauce, and soybean paste), edible oils (such as oils extracted from the above grains, such as peanut oil, soybean oil, sunflower seed oil, cottonseed oil, etc.), etc.

[0053] Specifically, the feed may be any compound, mixture or composition suitable for or intended to be ingested by an animal, for example, it may include grain, animal protein, etc.

[0054] Specifically, the above-mentioned animal protein can be, for example, fish meal, skim milk powder, whey, silkworm pupa powder, bone meal and the like.

[0055] The present invention also provides an aflatoxin degrading agent (or aflatoxin detoxifying agent), which comprises Hirsutella sinensis and / or Paecilomyces pyralis, and a biological composition comprising any one or both of them.

[0056] Specifically, Hirsutella sinensis, Paecilomyces pyogenes, and the biological composition have the above corresponding definitions of the present invention.

[0057] The present invention also provides a use of Hirsutella sinensis and / or Paecilomyces avicularis, and a biological composition of Hirsutella sinensis and / or Paecilomyces avicularis in the preparation of an aflatoxin degrading agent (or an aflatoxin antidote).

[0058] The present invention also provides a method for degrading aflatoxin (reducing or even eliminating the toxicity of aflatoxin), which comprises the steps of using Hirsutella sinensis and / or Paecilomyces avicularis, or using a biological composition of Hirsutella sinensis and / or Paecilomyces avicularis.

[0059] Specifically, aflatoxins, Hirsutella sinensis, Paecilomyces pyogenes, and biological compositions have the above-mentioned corresponding definitions of the present invention.

[0060] Specifically, the above method may include mixing the above biological composition with a material to be treated, and optionally, performing a biodegradation treatment step, wherein the material to be treated is a material suspected of containing aflatoxin and / or having an unknown level of aflatoxin (such as medicine, food, feed).

[0061] The inventors of the present invention have found through a large number of experiments that Hirsutella sinensis and Paecilomyces spp. can effectively degrade a variety of aflatoxins in a short period of time, and have very good application value in fields that require the degradation of aflatoxins, especially in the fields of medicine, food and feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] Figure 1 Shown are the kinetic results of AFB1 degradation by strain 8#. DETAILED DESCRIPTION

[0063] Unless otherwise defined, all scientific and technical terms used in the present invention have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention relates.

[0064] Hirsutella sinensis, Paecilomyces hepialiChen.

[0065] Aflatoxin (AF) is a bifuranoid toxin produced by certain strains of Aspergillus flavus and Aspergillus parasiticus. Aflatoxins can be divided into two categories: blue fluorescent B and green fluorescent G. B includes B1, B2, B2α, G includes G1, G2; and also includes some derivatives such as aflatoxin M1, M2, P1, Q, H1, GM, Aflatoxicol, etc.

[0066] In the present invention, "biological composition of Hirsutella sinensis" refers to a composition related to Hirsutella sinensis, and "biological composition of Paecilomyces sibiricum" refers to a composition related to Paecilomyces sibiricum (e.g., the number of China General Microbiological Culture Collection Center (CGMCC) is 3.15540, 3.17720, 3.15421), such as a preparation obtained by treating its cells, such as its seed liquid, fermentation liquid, bacterial suspension, intracellular liquid, cell fragment liquid, etc., and a preparation obtained by treating its seed liquid, such as seed liquid supernatant, fermentation liquid supernatant, fermentation liquid extract, seed liquid extract, etc. Therefore, "biological composition of Hirsutella sinensis or Paecilomyces sibiricum" comprises Hirsutella sinensis or Paecilomyces sibiricum (e.g., its live bacteria or cell fragments), intracellular substances or extracellular metabolites.

[0067]

[00136] Various publications, patents, and published patent specifications are cited herein, the disclosures of which are incorporated by reference in their entireties.

[0068] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0069] Example 1: Degradation of aflatoxin by different strains

[0070] According to the prior art CN109082382A and CN105670937A, different strains 1# to 9# of Chinese Hirsutella spp. (wherein strain 2# is strain L0106 described in CN109082382A) were obtained, a total of 9 strains of bacteria, and the obtained sequences were analyzed for similarity with the data stored in GenBank (DNA sequence database), and it was found that according to the principle of microbial molecular genetics identification, the homology based on the 18S rDNA sequence was higher than 95%, so the nine strains obtained by screening were all Chinese Hirsutella spp. The specific detection base sequences of strain 1#, strain 2#, strain 3#, strain 4#, strain 7#, strain 8# and strain 9# are shown in SEQ ID NOs: 1-7, respectively.

[0071] The Paecilomyces avicularis strains were purchased from China General Microbiological Culture Collection (CGMCC), affiliated to the Institute of Microbiology, Chinese Academy of Sciences, with CGMCC numbers 3.15540, 3.17720, and 3.15421.

[0072] 1. Preparation of seed solution of Chinese Hirsutella strains 1#~9# and Paecilomyces avicularis CGMCC 3.15540, 3.17720, and 3.15421

[0073] (1) Preparation of seed solution of Hirsutella sinensis strain

[0074] Take the slant of the Chinese Hirsutella spp. strain, scrape 1 / 4 of the slant hyphae and put it into PDB shake flask medium (200mL / 500mL), shake and culture at 120rpm, 15℃, and 12 days. Transfer to PDB medium at 10% of the inoculum amount and continue culturing for 4 days to obtain seed liquid.

[0075] (2) Preparation of seed solution of Paecilomyces avicularis

[0076] Take the slant of the Paecilomyces punctatus strain, scrape a 1.5cm*1.5cm fungus block and place it in PDB shake flask medium (200mL / 500mL), shake and culture at 150rpm, 25℃, and 5 days. Transfer to PDB medium at an inoculum rate of 5%, and obtain seed liquid after 2 days of culture.

[0077] 2. HPLC detection (all embodiments of the present invention use this method to detect aflatoxin)

[0078] Dichloromethane extraction: Centrifuge the sample at 12000rpm for 10min, take 1mL of the supernatant, add 1mL of methanol to mix, then add 1ml of dichloromethane to shake and mix, carefully draw the lower organic phase liquid, repeat the extraction three times, and combine the extracts. Blow dry with a nitrogen blower, wait for the dichloromethane to evaporate completely, then dissolve with 1mL of methanol, make up to volume, filter through a 0.22μm filter membrane, and detect with HPLC.

[0079] The chromatographic column is Pickering MYCOTOX TM C18 column (4.6*250mm, 5μm), mobile phase was methanol:acetonitrile:water (volume ratio 22:22:56), flow rate was 0.8mL / min, column temperature was 40℃, injection volume was 5μL, derivatization was performed using a photochemical derivatizer, detection was performed using a fluorescence detector, excitation wavelength was 360nm, and emission wavelength was 450nm.

[0080] 3. Degradation Experiment

[0081] The strains were respectively taken to prepare seed solutions in PDB medium, and 5 mL of the prepared strain seed solutions were added with 30 μl of AFB1 standard (acetonitrile as solvent) to an initial concentration of 18 ng / mL. The cultures were shaken at 14° C. and 120 rpm, and the AFB1 content was detected by HPLC-photoderivation.

[0082] AFB1 degradation rate = (initial sample AFB1 concentration - test sample AFB1 concentration) / initial sample AFB1 concentration * 100%.

[0083] The experimental results are shown in Table 1 below. Chinese Hirsutella strains 1#~9# and Paecilomyces pyri CGMCC 3.15540, 3.17720, and 3.15421 can all degrade AFB1:

[0084] Table 1 Degradation of AFB1 by different strains

[0085]

[0086]

[0087] Example 2: Strain 8# degrades aflatoxin

[0088] Take 5 mL of strain 8# seed liquid and add 30 μL of aflatoxin AFB1 standard solution (with acetonitrile as solvent), and add the same volume of PDB and seed liquid to the standard solution and acetonitrile as blank control, as shown in Table 2. Both the blank and experimental groups were cultured at 120 rpm and 15 °C, and 1 mL was sampled at 0, 48, 72, 96, and 120 h, respectively.

[0089] Table 2 Experimental design

[0090] Comparison 1 5mL blank medium + 30μL standard sample Comparison 2 5mL seed solution + 30μL acetonitrile Low concentration experimental group 1 5mL seed solution + 30μL standard sample High concentration experimental group 2 5mL seed solution + 50μL standard sample

[0091] Note: The original concentration of the standard sample was 3ppm, the AFB1 concentration in the low-concentration experimental group 1 was 17.89ppb, and the AFB1 concentration in the high-concentration experimental group 2 was 29.70ppb.

[0092] The aflatoxin content was detected by referring to the method in Example 1. The results showed that the seed solution of Hirsutella chinensis could degrade the aflatoxin standard sample, and the degradation rate of the low-concentration sample with 90 ng added was 98% in 96 hours, and the degradation rate of the high-concentration sample was 100% in 120 hours. The kinetic results of the degradation of aflatoxin by the high-concentration sample are shown in Figure 2. Figure 1 shown.

[0093] Distinguishing between degradation and desorption

[0094] Take samples from the low and high concentration experimental groups, centrifuge at 12000rpm for 10min, take the supernatant and extract with chloroform; at the same time, add PBS solution (pH 7.4) of the same volume as the supernatant to the bacterial precipitate into the centrifuge tube, resuspend the bacteria, centrifuge at 12000rpm for 10min, and discard the supernatant.

[0095] An equal volume of methanol solution was added to the above precipitate, the mixture was suspended and mixed, ultrasonicated at 40KHZ for 30 min, and filtered through a 0.22 μm filter membrane to obtain the precipitate extract.

[0096] The aflatoxin content in the bacterial precipitation extract and supernatant samples was tested respectively. The results showed that no aflatoxin was detected in the seed liquid supernatant and the bacterial extract, which means that the effect of strain 8# on aflatoxin is degradation rather than adsorption.

[0097] Example 3: Strain 8# degrades aflatoxins AFG2, AFG1, AFB2 and AFB1

[0098] The seed solution was prepared in the same manner as in Example 1. 4.95 mL of the seed solution was added with 50 μl of mixed standards AFB1 (1000 ppb), AFB2 (300 ppb), AFG1 (1000 ppb) and AFG2 (300 ppb) to an initial concentration of 10 ppb, 3 ppb, 10 ppb and 3 ppb, respectively. The mixture was stirred at 14° C. and 120 rpm. PDB medium was used instead of the seed solution as a control. After oscillating for 144 h, the mixture was extracted with dichloromethane and detected by HPLC-photoderivation.

[0099] The results showed that strain 8# could effectively degrade AFG2, AFG1, AFB2 and AFB1 at 144 h, with residual rates of 23%, 0, 10% and 0, respectively.

[0100] Example 4: Degradation experiment of AFB1 by different components in the seed solution of strain 8#

[0101] The seed solution was prepared in the same manner as in Example 1;

[0102] 1. Supernatant

[0103] Take 54 mL of seed solution, centrifuge and obtain the supernatant.

[0104] 2. Bacterial suspension:

[0105] The bacterial precipitate was washed twice with PBS at pH 7.4 and then suspended with the same volume of PBS to obtain a bacterial suspension.

[0106] 3. Preparation of intracellular fluid and cell fragments:

[0107] Take 15 mL of bacterial suspension, grind it, centrifuge the grinding solution, take the supernatant and record it as intracellular fluid, and resuspend the precipitate with 15 mL of PBS and record it as cell debris.

[0108] 4. Different components degrade AFB1:

[0109] Take 5 mL of the seed solution, supernatant, bacterial suspension, intracellular fluid and cell debris prepared above, and add 30 μL of AFB1 standard to make the initial AFB1 concentration 18 ng / mL. Incubate at 15°C, 120 rpm with shaking, take samples at 96 h, and detect by HPLC-photoderivation.

[0110] The experimental results are shown in Table 3 below.

[0111] Table 3 Experimental results of degradation of AFB1 by different components in 96h

[0112] Components Degradation rate Blank control 0％ Seed Liquid 100％ Supernatant 3％ bacterial suspension 100％ Intracellular fluid 6％ Cell debris 33％

[0113] The degradation effect of the supernatant in which the bacteria are basically removed and the intracellular fluid without live bacteria is very low, that is, the Chinese hair spore itself has a degradation effect, and the cell fragments may contain live bacteria, thus having a certain degradation effect.

[0114] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

[0115] The aforementioned embodiments and methods described in the present invention may be varied based on the ability, experience and preference of those skilled in the art.

[0116] In the present invention, merely listing the steps of the method in a certain order does not constitute any limitation on the order of the method steps.

Claims

1. Application of Hirsutella chinensis and / or Paecilomyces spp. in degradation of aflatoxins.

2. Application of Hirsutella chinensis and / or Paecilomyces pyogenes in reducing or eliminating the toxicity of aflatoxin.

3. The use according to claim 1 or 2, characterized in that: The Chinese Hirsutella sinensis achieves the degradation, reduction or elimination through a non-physical method.

4. Use of a biological composition of a bacterial strain in degrading aflatoxin, wherein the bacterial strain is Hirsutella sinensis and / or Paecilomyces pyogenes.

5. Use of a biological composition of a bacterial strain in reducing or eliminating the toxicity of aflatoxin, wherein the bacterial strain is Hirsutella sinensis and / or Paecilomyces pyralis.

6. The use according to claim 4 or 5, characterized in that The composition comprises the cells of Hirsutella sinensis strain or Paecilomyces avicularis strain, preferably living cells; Preferably, the composition comprises one or more of the seed liquid, fermentation liquid, bacterial suspension, supernatant, intracellular liquid, cell debris liquid, seed liquid extract, fermentation liquid extract, and bacterial powder of the strain, preferably one or more of the seed liquid, fermentation liquid, and bacterial suspension.

7. The use according to any one of claims 1 to 6, characterized in that: The aflatoxin is selected from: aflatoxin B1, B2, B2α, G1, G2, aflatoxin derivatives M1, M2, P1, Q, H1, GM, aflatoxin alcohol, in particular AFB1, AFB2, AFG1, AFG2.

8. Application of Hirsutella sinica and / or Paecilomyces spp. in medicine, food or feed.

9. An aflatoxin antidote comprising Hirsutella chinensis and / or Paecilomyces pyralis; Preferably, the aflatoxin antidote comprises one or more of the seed liquid, fermentation liquid, bacterial suspension, supernatant, intracellular liquid, cell debris liquid, seed liquid extract, fermentation liquid extract, and bacterial powder of the strain, preferably one or more of the seed liquid, fermentation liquid, and bacterial suspension.

10. A method for reducing or eliminating the toxicity of aflatoxin, comprising the step of using Hirsutella chinensis and / or Paecilomyces pyralis; Preferably, the method comprises the step of using one or more of seed liquid, fermentation liquid, bacterial suspension, supernatant, intracellular liquid, cell debris liquid, seed liquid extract, and bacterial powder of Hirsutella sinensis and / or Paecilomyces aviculare, preferably one or more of seed liquid, fermentation liquid, and bacterial suspension.

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