17004 results about "Bacilli" patented technology

The invention discloses a microbial compound bacterial fertilizer for improving saline alkali soil and increasing the acting yield. A preparation method of the microbial compound bacterial fertilizer comprises the following steps: preparing a microbial agent containing phosphate-solubilizing bacteria, potassium-solubilizing bacteria, nitrogen-fixing bacteria, bacillus subtilis and actinomycetes; the preparation method comprises the following steps: preparing a sodium alginate solution, adding nano-hydroxyapatite and a microbial agent, uniformly mixing, dropwise adding a calcium chloride solution, and embedding an inner layer to obtain microbial embedded particles; uniformly mixing biochar, humic acid and attapulgite, taking a 2-3% HPMC solution as a binder, and carrying out middle-layer coating, so as to obtain prefabricated microbial fertilizer particles; and coating the chitosan-sodium alginate-nano ZnO outer layer to obtain the microbial compound bacterial fertilizer. The microbial compound bacterial fertilizer disclosed by the invention adopts a multi-layer and multifunctional collaborative design concept, realizes efficient protection and slow release of functional microorganisms in a saline-alkali environment, remarkably improves the physicochemical properties of soil and promotes the growth of crops.

The invention provides bacillus and application thereof, the bacillus is preserved in Guangdong Microbial Culture Collection Center on June 6, 2025, and the preservation number is GDMCC No.66483; one application of the bacillus is to prepare a preparation for promoting growth and / or resisting diseases of plants, and the other application is to use the bacillus for inhibiting microorganisms; the bacillus can effectively and enduringly inhibit the growth of phytopathogen, and can be used as a broad-spectrum antibacterial biological antibacterial agent to enhance the resistance of plants and promote the growth of the plants.

The invention discloses bacillus velezensis GX0003742 and biocontrol application thereof, and belongs to the technical field of microorganism application. The invention discloses bacillus velezensis GX0003742 with a broad-spectrum bacteriostatic effect, which is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 14, 2024, and the preservation number is CGMCC No.31981. The bacillus velezensis GX0003742 has the advantages that the bacillus velezensis GX0003742 can be used for preparing the bacillus velezensis GX0003742; the strain can obviously inhibit growth of colletotrichum basjoo mycelia and obviously reduce occurrence of postharvest diseases of bananas, has an inhibiting effect on various pathogenic bacteria, has a good insecticidal effect on auricular bean root-knot nematode, and provides a new strain resource for biological prevention and control of plant diseases.

The invention relates to bacillus velezensis SMB1 and application thereof in prevention and treatment of plant diseases, and belongs to the technical field of biological prevention and treatment of plant diseases. The strain is preserved in the China Center for Type Culture Collection on November 27, 2024, the preservation address is Luojia mountain, Luojia mountain, eight road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M20242647. The bacillus velezensis SMB1 provided by the invention has a remarkable bacteriostatic effect on plant pathogenic fungi, plant pathogenic oomycetes, plant pathogenic bacteria and animal pathogenic bacteria, has an extremely good broad-spectrum bacteriostatic function, has a great application potential in biological control of plant diseases, can reduce the use of chemical pesticides, and has a good application prospect. The pesticide residue in agricultural products is reduced, the environment is protected, and the urgent demand on pollution-free agricultural product production at present is met.

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as ZZ1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the preservation center is numbered as CGMCC No.31374, and the preservation time is July 19, 2024. The bacillus ZZ1 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus ZZ1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a plant lactobacillus XD117, application of the plant lactobacillus XD117 in fermentation of astragalus membranaceus, an astragalus membranaceus fermentation product and a preparation method of the astragalus membranaceus fermentation product. The bacterial strain is lactobacillus plantarum XD117 and is preserved in the China Center for Type Culture Collection, and the preservation number of the bacterial strain is CCTCC NO: M 20241730. The preparation method of the astragalus mongholicus fermentation product comprises the following steps: inoculating the lactobacillus plantarum XD117 into astragalus mongholicus enzymatic hydrolysate, and fermenting in a fermentation temperature range suitable for the lactobacillus plantarum XD117 to obtain the astragalus mongholicus fermentation product. The total content of calycosin, formononetin, calycosin glucoside and formononin in the astragalus mongholicus fermentation product is high, and the astragalus mongholicus fermentation product has a very good antioxidant effect.

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention provides a bifidobacterium animalis subsp. Lactis strain B21247 with a hypoglycemic function and application thereof, and belongs to the technical field of functional microorganisms. The invention relates to a bifidobacterium animalis subsp. Lactis strain B21247 with a hypoglycemic function. The preservation number of the bifidobacterium animalis subsp. Lactis strain B21247 is GDMCC No: 65075. The strain B21247 has a good intestinal colonization function, and has the activity of inhibiting alpha-glucosidase in vitro. Meanwhile, a type 2 diabetes mouse model is used as an experimental model to verify that the strain B21247 achieves the effect of reducing blood sugar by adjusting glycosylated hemoglobin, reducing pancreatic beta cell damage and increasing secretion of intestinal GLP-1, and a new thought is provided for reducing blood sugar or treating diabetes.

The invention discloses a primer probe combination and a kit for detecting genital tract pathogenic microorganisms and application. The primer probe combination comprises primer probe sequences as shown in SEQ ID NO.1-SEQ ID NO.96, the genital tract pathogens comprise 27 pathogens capable of causing genital tract diseases, such as ureaplasma parvum, ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium, candida albicans, candida glabrata, candida tropicalis, candida auricula, candida krusei and candida duberlin, as well as lactobacillus crispatus, lactobacillus jensenii and lactobacillus gasseri; the lactobacillus lactis comprises four common vaginal lactobacillus lactis, and also comprises a human-derived interior label. On the basis of a fluorescent quantitative PCR technology, the kit can be used for simultaneously detecting 27 pathogens capable of causing genital tract diseases and 4 common lactobacillus vaginalis in an in-vitro sample, not only can cover main pathogens of genital tract infection, but also meets the evaluation on the ecological environment of the vagina, the detection time is shortened to about 40 minutes, and meanwhile, the kit has relatively good specificity and sensitivity.

The invention discloses lactobacillus kefiranofaciens IMAU12319 and application thereof, belongs to the technical field of microorganisms, and particularly relates to lactobacillus kefiranofaciens IMAU12319 which is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 8, 2024, the preservation address is No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, and the number is CGMCC No.31224. The lactobacillus kefiranofaciens IMAU12319 has the advantages that the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319, and the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319; the invention also relates to an application of the lactobacillus kefiranofaciens IMAU12319 in preparation of a probiotic agent capable of resisting gastrointestinal digestion. The strain disclosed by the invention not only shows good gastrointestinal tract endurance capacity, but also shows relatively strong potential in the aspects of intestinal adhesion, oxidation resistance and bacteriostasis, and has a wide application prospect in leavening agent strains.

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention relates to the field of self-repairing concrete, in particular to high-strength self-repairing concrete and a preparation method thereof. The high-strength self-repairing concrete comprises the following substances in parts by weight: 45-60 parts of cement; 100 to 120 parts of recycled coarse aggregate; 65 to 80 parts of fine aggregate; 10 to 20 parts of water; 0.1 to 0.3 part of a water reducing agent; the recycled coarse aggregate is the recycled coarse aggregate immobilized with self-repairing microorganisms, and the self-repairing microorganisms comprise at least one of bacillus subtilis, bacillus megatherium and bacillus pasteurii. The concrete crack self-repairing capability is endowed through the immobilized self-repairing microorganisms. The microorganisms form resting spores in the concrete, when cracks are generated and water permeates into the cracks, the microorganisms are activated and metabolize a calcium source and urea in the surrounding environment, calcium carbonate crystals are generated through the mechanism that the microorganisms induce carbonate precipitation, the cracks are filled, and the structural integrity is recovered. The process does not need external intervention, and the mechanical structure and mechanical properties of the concrete structure can be remarkably improved.

The invention discloses a bio-organic fertilizer for repairing heavy metal cadmium in soil and a preparation method of the bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials in parts by weight: 7-12 parts of a compound microbial agent, 30-40 parts of modified charcoal, 3-8 parts of sodium alginate, 6-8 parts of a chelating agent and 60-80 parts of decomposed edible fungus residues. The compound microbial agent is prepared from potassium-solubilizing paenibacillus mucilaginosus MSSW02, pseudomonas plecoglossicida and bacillus aryabhattai; the chelating agent is composed of calcium humate and nano-hydroxyapatite according to the mass ratio of 100: (3-5). The multi-component synergistic effect is achieved, the content of heavy metal in soil is effectively reduced, especially the content of heavy metal Cd, meanwhile, rich organic matter is contained, the physical and chemical properties of the soil can be remarkably improved, the water and fertilizer retention capacity of the soil is improved, and crop growth is promoted; the remediation effect on medium-light cadmium polluted farmland soil is good, and the growth promoting effect on crops is remarkable.

The invention discloses bacillus cereus and application thereof, and belongs to the technical field of microorganisms. The bacillus cereus SNF7 is separated from a saline-alkali soil sample, has the functions of resisting salt, fixing nitrogen and promoting growth, has an obvious inhibition effect on some common soil-borne diseases in agriculture, such as pathogenic bacteria of peanut fruit rot, tomato root rot and the like, has multiple functions of resisting salt, fixing nitrogen, biologically preventing and controlling and the like, and has a wide application prospect. The method can be widely applied to crop planting in large saline-alkali soil in China.

The invention relates to the technical field of microorganisms, in particular to plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid and application of the plant lactobacillus MPs-68. The provided plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid is a marine-derived natural strain, the preservation number is CGMCC No.31976, the plant lactobacillus MPs-68 has a remarkable adsorption effect on microplastics polystyrene and polyethylene, has the function of degrading uric acid, and is high in oxidation resistance, gastric acid resistance, cholate resistance and intestinal colonization capacity, and the plant lactobacillus MPs-68 can be used for adsorbing microplastics and degrading uric acid. The method has important application value in the fields of food or health care products for assisting adsorption of micro-plastics and reduction of uric acid and the like. The invention also provides a microbial agent, a probiotic product, a functional food, a fermentation product, a microecological preparation, a microplastic adsorbent and a microplastic adsorbent compounded with the lactobacillus brevis MPA01 (the preservation number is CGMCC No.31977) containing the phytobacterium plantarum MPs-68 capable of adsorbing the microplastics and degrading the uric acid, and the microplastic adsorbent has the effects of adsorbing the microplastics, degrading the uric acid and the like.

The invention provides a plant lactobacillus capable of promoting mineral absorption and transport and application of the plant lactobacillus. The invention provides a plant lactobacillus of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32404. The invention also provides a culture, a sterilized solution, a supernatant, a filtrate, a diluent, a precipitate or freeze-dried powder, a composition and a probiotic preparation containing the plant lactobacillus, and related applications and methods thereof.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

Provided is a Limosilactobacillus fermentum VB216, which has the deposit number of CGMCC No. 26578 in the China General Microbiological Culture Collection Center, and has the deposit date of 20 February 2023. The Limosilactobacillus fermentum has inhibitory activities with regard to a variety of pathogenic bacteria and excellent activity for degrading arginine. The present invention can be used in the preparation of products, such as food, health-care food, medicine and feed, for preventing and / or relieving and / or treating arginine metabolism-related diseases, such as hyperarginemia, malignant tumor treatment, allergic reactions, eczema, pruritus and abdominal spasm, and has great application prospects.

The invention discloses a plant lactobacillus with a bacteriostatic effect and application of the plant lactobacillus. The lactobacillus plantarum is named as lactobacillus plantarum C07, the preservation number is CCTCC NO: M20242086, the preservation unit is China Center for Type Culture Collection, the preservation time is September 26, 2024, and the preservation address is Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The strain can produce lactic acid and indole-3-lactic acid at high yield, has a very strong antibacterial effect on multiple putrefying and pathogenic microorganisms such as staphylococcus aureus, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella and mould, and is a broad-spectrum antibacterial microorganism.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide for inhibiting drug-resistant acinetobacter baumannii as well as a preparation method and application of the antibacterial peptide. The antibacterial peptide is cd77AHP-2, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The antibacterial peptide cd77 obtained through artificial chemical synthesis is modified, distribution of cationic amino acid is optimized, and the antibacterial activity of the antibacterial peptide is remarkably improved. The antibacterial peptide cd77AHP-2 has a remarkable antibacterial effect on acinetobacter baumannii which are gram-negative bacteria and multi-drug resistant acinetobacter baumannii, and the antibacterial peptide cd77AHP-2 has a remarkable antibacterial effect on the acinetobacter baumannii which are gram-negative bacteria. The antibacterial peptide cd77AHP-2 provided by the invention has relatively low cytotoxicity to human bronchial epithelial cells, and has the potential of preparing a drug for resisting drug-resistant acinetobacter baumannii. The invention provides a novel functional polypeptide for the field of antibacterial peptides, and the novel functional polypeptide has important practical application value.

The invention relates to lactobacillus rhamnosus CBBM005 and application thereof in products for balancing intestinal flora, improving immunity and improving oral cavity, and belongs to the technical field of microorganisms. The lactobacillus rhamnosus CBBM005 is separated from human intestinal secretion, the lactobacillus rhamnosus CBBM005 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation time is March 20, 2025, and the preservation number is CGMCC NO.33904. The lactobacillus rhamnosus CBBM005 has the characteristics of balancing intestinal flora, improving immunity and improving oral cavity.

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus mobilis DG-7 and application thereof in inhibiting bolting of angelica sinensis and improving disease resistance of angelica sinensis. The invention provides a strain of bacillus mobilis DG-7, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31089. The bacillus mobilis DG-7 and the microbial inoculum thereof provided by the invention can play roles in inhibiting early bolting of angelica sinensis and improving disease resistance by increasing the content of plant endogenous melatonin and salicylic acid, reducing the content of abscisic acid, auxin and gibberellin, reducing the content of total phenolic acid and reducing the expression of photoperiod-related genes. In addition, the bacillus mobilis DG-7 does not solubilize phosphorus, so that the promotion effect of high-concentration phosphorus on early bolting is effectively avoided; the laccase activity of the bacillus mobilis DG-7 is high, polyphenol substances can be effectively removed, and the bolting rate can be reduced.

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.

The invention discloses a carbonyl reductase, a mutant and an application of the carbonyl reductase in synthesis of a chiral alcohol compound. The carbonyl reductase mutant is obtained by performing single-site or multi-site mutation on the 96th site, the 145th site, the 202nd site or the 206th site of the amino acid sequence of the carbonyl reductase LbCR derived from lactobacillus brevis as shown in SEQ ID No.2. The activity and stability of the carbonyl reductase LbCR mutant are obviously improved, and the carbonyl reductase LbCR mutant has a wide substrate spectrum. Compared with a wild type, the mutant LbCRM4 has the advantages that the enzyme activity is improved by 86.3 times, the substrate conversion rate is greater than 99%, the e.e.p value of the product is always kept at 99.5% or above, the space time yield reaches 1288.9 g / L / d, and the half-life period at 40 DEG C is 101.4 h and is improved by 83.5 times. The LbCR mutant has an industrial application prospect.

The invention relates to the technical field of microorganisms, and in particular relates to application of a strain of lactobacillus mucus TG018. The invention further relates to a preparation method of the lactobacillus mucus TG018. The preservation number of the lactobacillus mucus TG018 is CCTCC (China Center for Type Culture Collection) No: M 2023514; the lactobacillus mucus TG018 can be used for producing nicotinic acid, serving as an antioxidant and / or preparing the antioxidant, or can be applied to medicines for reducing uric acid.

The invention discloses a preparation method of a curing agent with biological self-healing capability, which comprises the following steps: preparing a bacillus pasteurii bacterial solution, preparing the bacillus pasteurii bacterial solution into an endophytic spore solution, immersing polyvinyl alcohol fibers into a sodium alginate / endophytic spore solution, taking out the polyvinyl alcohol fibers, immediately immersing the polyvinyl alcohol fibers into a calcium acetate solution, drying the polyvinyl alcohol fibers to obtain core fibers with a hydrogel coating, and curing the core fibers with the hydrogel coating to obtain the curing agent with biological self-healing capability. The preparation method comprises the following steps: taking magnesium phosphate cement as a raw material, immediately immersing the magnesium phosphate cement into a chloroform solution of a polystyrene and polylactic acid polymer blend, drying to obtain a coated microbial liquid, premixing urea and glucose, doping the premixed urea and glucose into the magnesium phosphate cement, uniformly stirring, adding the coated microbial liquid, and uniformly stirring to obtain the product. According to the invention, phosphate cement is adopted as the curing agent, has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility and the like, and compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is especially suitable for reinforcing foundation soil.

The invention discloses ultra-high performance concrete and a preparation method thereof. The ultra-high performance concrete comprises the following components in percentage by mass: 15%-25% of cement, 3%-6% of nano SiO2, 30%-35% of fly ash or steel slag powder, 35%-40% of recycled aggregate, 1%-4% of fiber, 0.5% of a microbial remediation agent and 1%-3% of nano TiO2. The preparation method comprises the following steps: S1, pretreating the recycled aggregate; and S2, packaging the microbial remediation agent: mixing the bacillus pasteurii spores with calcium lactate, and wrapping the mixture in calcium phosphate microspheres. S3, step-by-step stirring; S3.1, dry mixing: uniformly stirring the cement, the fly ash, the nano SiO2, the nano TiO2 and the recycled aggregate; s3.2, wet mixing is carried out, water solution fibers containing the polycarboxylic acid water reducing agent are added, uniform stirring is carried out, and the water-binder ratio of the water solution containing the polycarboxylic acid water reducing agent is 0.18; s3.3, final mixing: adding the packaged microbial remediation agent, and uniformly stirring; and S4, directional curing: performing steam curing at 80 DEG C for 48 hours, performing ultraviolet irradiation, and performing constant-temperature and constant-humidity curing for 7 days. The ultra-high performance concrete has the effects of low cost, low carbon emission, multi-function integration and repeatable crack self-repairing.

The invention relates to a weight-losing enzyme and a preparation method thereof, and belongs to the technical field of biology, and the weight-losing enzyme comprises a fruit enzyme, a vegetable enzyme, a seed enzyme and a lotus leaf extract; the fruit enzyme is obtained by performing enzymolysis on mixed fruit pulp of apples, lemons, grapefruits and haws through pectinase, performing enzymolysis through pepsase and performing co-fermentation through bifidobacterium longum and lactobacillus acidophilus; the vegetable enzyme is obtained by performing enzymolysis on mixed vegetable pulp of wax gourd pulp, celery and spinach through pectinase, performing enzymolysis through pepsin and performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis; the seed enzyme is obtained by carrying out trypsin enzymolysis on mixed powder of semen raphani and phaseolus calcaratus, then carrying out nattokinase enzymolysis and then fermenting with bifidobacterium breve; the lotus leaf extract is obtained by sequentially carrying out enzymolysis on lotus leaves through hemicellulase, snail protease and nattokinase. All the components are matched for use, so that the weight-losing food has the effects of promoting digestion, inhibiting fat absorption, regulating intestinal flora, promoting metabolism and the like, and has a good weight-losing effect.

The invention provides an application of a fermentation lactobacillus mucus strain A21196 in preparation of a product for resisting anxiety and improving memory and concentration, and belongs to the technical field of biological medicines. Animal experiments show that the fermented lactobacillus mucus strain A21196 has the effects of resisting anxiety, resisting depression, improving cognitive impairment function and improving memory concentration, and provides a new means for preventing and / or treating nerve diseases related to anxiety disorder, depression and cognitive impairment.