518 results about "Recombinant expression" patented technology

The invention relates to the technical field of biology, in particular to a recombinant fusion protein, polynucleotide for coding the recombinant fusion protein, a recombinant expression plasmid containing the polynucleotide, an engineered recombinant host cell and a method for preparing target polypeptide by using the recombinant fusion protein. The recombinant fusion protein comprises tag polypeptide-target polypeptide-(linker 1-linker x-linker 2-target polypeptide) n from N terminal to C terminal, x is the number of linkers, x is 0 or 1, n is a positive integer not less than 1, the tag polypeptide is SEQ ID NO.1 or SEQ ID NO.2, the linker 1 contains a Kex2 protease enzyme cutting site and a carboxypeptidase B enzyme cutting site, and the linker 1 contains a Kex2 protease enzyme cutting site and a carboxypeptidase B enzyme cutting site. And the connecting peptide 2 contains a WELQ protease enzyme cutting site. The recombinant fusion protein disclosed by the invention is expressed in an inclusion body form, the synthesis process is simplified, the target polypeptide can be prepared with high yield and high purity by using the recombinant fusion protein disclosed by the invention, and the production cost can be reduced from multiple dimensions.

The invention discloses a construction method and application of a chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain, the construction method comprises the following steps: carrying out gene modification on sweeteners Thaumatin and Brazzein, optimizing preference codons of chlamydomonas reinhardtii to synthesize CrThaumatin and CrBrazzein encoding genes, cloning the encoding genes into an expression vector pGM6, introducing the recombinant expression vector into a chlamydomonas reinhardtii wild-type chlamydomonas sp. Algal strain, and carrying out high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii. And screening by using a paromomycin resistance marker carried by the expression vector to successfully obtain an engineering algal strain. According to the present invention, the engineering strain is subjected to fermentation production to obtain the dry and stable Chlamydomonas reinhardtii powder rich in the target sweet protein, and the protein purification oral test results show that the sweet taste of the Chlamydomonas reinhardtii powder is 3-5 times of the sweet taste of the natural Thaumatin and Brazzein protein;

The present invention relates to: a receptor tyrosine kinase like orphan receptor 1 (ROR 1) antibody or an antigen-binding fragment thereof; a nucleic acid encoding same; a recombinant expression vector carrying the nucleic acid; a host cell transinfected with the recombinant expression vector; a method for preparing the antibody or the antigen-binding fragment thereof; a bi- or multi-specific antibody bearing the antibody or the antigen-binding fragment thereof; an immune cell-engaging bi- or multi-specific antibody; an antibody-drug conjugate (ADC) in which the antibody or the antigen-binding fragment thereof is bound to a drug; a chimeric antigen receptor (CAR) containing the scFv of the antibody as an antigen-binding site of an extracellular domain; an immune cell having the chimeric antigen receptor introduced thereinto; a composition for combination therapy including the antibody or the antigen-binding fragment thereof; a composition for preventing or treating cancer; and a method for preventing or treating cancer.

The invention discloses a 5 'UTR library, a recombinant expression vector and a genetically engineered bacterium for improving the yield of folamin in pathogenic bacterium Badei and application of the 5' UTR library and the genetically engineered bacterium, and belongs to the technical field of microbial genetic engineering. The method comprises the following steps: designing an SD region based on a 16S rRNA (ribosomal Ribonucleic Acid) 3'end complementary sequence of a Buddei pathogenic bacterium XBD101 serving as an original strain, regulating and controlling the number of bases between the SD sequence and an initiation codon AUG and optimizing an UTR secondary structure to construct a library containing 14 UTR sequences, and replacing three sequences N4A, N7A and N18A with the strongest translation ability in the library to the UTR region of a folamin synthesis key gene fclC, so as to obtain the folamin peptide. And finally, the capacity upgrading of the folamin chassis strain is realized, and an important support is provided for the industrial production of agricultural antibiotics.

The invention discloses recombinant escherichia coli with high yield of N-acetylneuraminic acid and application of the recombinant escherichia coli, and relates to the technical field of biological genetic engineering. The invention relates to a recombinant escherichia coli, which is characterized in that the escherichia coli is taken as a host, and free expression of an N-acetylmannosamine epimerase gene yihS from Streptomyces xiamenensis or an N-acetylmannosamine epimerase gene ce3 from Bacteroides polymorpha and an exogenous N-acetylneuraminic acid lyase gene nano A is carried out; and carrying out recombinant expression on N-acetyl hexosamine 1-kinase nahK, a UDP-N-acetyl glucosamine pyrophosphorylase gene glmU and a UDP-N-acetyl glucosamine-2-epimerase gene neuC in the other synthetic route of the ManNAc. According to the recombinant escherichia coli with high yield of N-acetylneuraminic acid, the yield of N-acetylneuraminic acid can reach 23.08 g / L under a shake flask fermentation condition; a two-stage batch feeding strategy is adopted, the yield of N-acetylneuraminic acid in a 5L fermentation tank reaches 71.25 g / L, the molar conversion rate of GlcNAc reaches up to 57.60%, and the method has the potential of industrial application.

The invention discloses a high-flux and rapid chromatin immunoprecipitation sequencing method based on a HiBiT tag and a Tn5 enzyme, and belongs to the technical field of biology. According to the method, the high affinity characteristic of a HiBiT tag system is combined with a Tn5 enzyme in-situ library building technology, firstly, a DNA binding protein recombinant expression strain containing a HiBiT tag is constructed and subjected to induced expression, after formaldehyde crosslinking and cell lysis, an Anti-HiBiT antibody is used for rapid immunoprecipitation, and a Tn5 enzyme library is constructed; tn5 transposase is directly loaded on the magnetic beads combined with the protein and DNA fragment compound to complete fragmentation and linker connection, so that the construction of a next-generation sequencing library is quickly carried out, and the total consumed time from immunoprecipitation to the completion of library construction is less than or equal to 5 hours. Meanwhile, automatic treatment is realized through a micro experiment board (the reaction system is less than or equal to 200 microliters) and a liquid workstation, the experiment cost and the sample dosage are reduced, 48 transcription factors and 48 control samples can be synchronously treated, the efficiency is greatly improved, and an efficient tool is provided for microbial transcription regulation and control research.

The invention provides an anti-white spot syndrome virus peptide derived from litopenaeus vannamei, and belongs to the technical field of biology. The amino acid sequence of the anti-white spot syndrome virus peptide provided by the invention is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO.2. The invention further provides a recombinant expression vector and host bacteria containing the anti-white spot syndrome virus peptide gene, and application of the recombinant expression vector and host bacteria in anti-white spot syndrome virus drugs, vaccines, feeds and feed additives, and the recombinant expression vector and host bacteria have wide application prospects and economic benefits.

The invention discloses a method for efficiently expressing laccase by regulating endoplasmic reticulum stress reaction of coprinus cinereus, and belongs to the field of gene engineering and fermentation engineering. According to the invention, by regulating endoplasmic reticulum stress reaction action genes der1, Anhac1 and Cchac1 of filamentous fungi coprinus cinereus, recombinant extracellular expression of laccase Lcc5 is finally improved and regulated. Wherein the extracellular laccase activities of the recombinant bacteria can be respectively improved by 18%, 70% and 26% through interference of der1, overexpression of Anhac1 and overexpression of Cchac1, and the obtained extracellular laccase activities are respectively 31.4 U / mL, 45.1 U / mL and 33.6 U / mL, which is the highest level of coprinus cinereus homologous recombination expression laccase Lcc5 reported at present. The invention firstly indicates that the regulation and control of the coprinus cinereus endoplasmic reticulum stress reaction can promote the efficient expression of laccase in coprinus cinereus, and the invention further promotes the development of a coprinus cinereus expression system.

The invention relates to the field of biological medicine preparation, and particularly provides liraglutide precursor peptide tandem recombinant fusion protein, polynucleotide, recombinant expression plasmid, engineered host cell and a method for preparing target polypeptide. The recombinant fusion protein is fusion peptide-target polypeptide-(linker peptide-target polypeptide) n from the N terminal to the C terminal, n is a positive integer from 4 to 6, the fusion peptide is SEQ ID NO.1, the target polypeptide is liraglutide precursor peptide, the linker peptide comprises a spacer peptide and a protease restriction enzyme cutting site, the spacer peptide is SEQ ID NO.2, the isoelectric point of the recombinant fusion protein is 4.6-4.7, and the average hydrophilic value is-0.780--0.765. The proportion of the target polypeptide in the recombinant fusion protein is high, use of solvents under extreme conditions is avoided in the production process, the enzyme digestion efficiency is high, and the product purity and yield are high.

The invention discloses a limonium bicolor gene LbZFP8 and application thereof, and relates to the technical field of biological genetic engineering. The invention relates to a limonium bicolor gene LbZFP8 and application thereof. The limonium bicolor gene LbZFP8 is a gene for coding a protein composed of an amino acid sequence as shown in SEQ ID NO: 1; the biological material is recombinant DNA, an expression cassette, a transposon, a plasmid vector, a virus vector or engineering bacteria. The gene provided by the invention can significantly improve the salt tolerance and oxidation resistance of a target plant by transforming plant cells. Experiments show that under the salt stress condition, the survival rate of the LbZFP8 overexpressed arabidopsis thaliana plant is increased by about 45% compared with that of a control group. Meanwhile, the compound plays a key role in regulating and controlling an active oxygen scavenging system, and the activity of SOD and POD enzymes can be effectively enhanced. The technology provides an important gene resource for cultivating new varieties of crops with strong stress resistance, and has a wide application prospect.

The invention discloses a cellobiose 2-epimerase mutant as well as a gene, an expression vector, recombinant bacteria, a preparation method and application thereof. The mutant is obtained by performing the following point mutation on an amino acid sequence as shown in SEQ ID No.1: S12A, S24A, G131A, G176A, G224A, K46R, K69R, K72R, K91R, K103R, K201R, K219R, K223R, K241R, K263R, K266R, K273R, K284R, K325R, K335R, K349R and K387R. The invention further discloses a preparation method of the mutant. The invention further discloses a gene segment, a plasmid and a recombinant bacterium for recombinant expression of the mutant, and the gene segment, the plasmid and the recombinant bacterium are used for recombinant expression preparation of the mutant. The mutant can be used for continuously and stably catalyzing and preparing lactulose at high temperature, is relatively good in thermal stability and has a relatively good industrial application prospect.

The invention relates to the field of biological medicine preparation, and particularly provides canagliflozin precursor peptide tandem recombinant fusion protein, polynucleotide, recombinant expression plasmid, engineered host cell and a method for preparing target polypeptide. The recombinant fusion protein is a fusion peptide-target polypeptide-(linker peptide-target polypeptide) n from an N terminal to a C terminal, n is a positive integer of 4-6, the fusion peptide is SEQ ID NO.1 or SEQ ID NO.2, the target polypeptide is a canagliptin precursor peptide, the linker peptide comprises a spacer peptide and a protease restriction enzyme cutting site, the spacer peptide is SEQ ID NO.3, the isoelectric point of the recombinant fusion protein is 5.5-6.5, and the average hydrophilic value is-0.85--0.96. The invention creatively provides a method for preparing the canagliflozin precursor peptide by using a gene recombination technology, the proportion of the target polypeptide in the recombinant fusion protein is high, the use of a solvent under extreme conditions is avoided in the production process, the enzyme digestion efficiency is high, and the product purity and yield are high.

The present invention relates to an artificial hybrid promoter, and more particularly, to an artificial hybrid promoter including an upstream activation sequence of GAL10 gene and a core promoter sequence of PGK gene, a recombinant expression vector including same, a host cell, and a method of producing a target protein using the host cell.

The present invention describes mRNA usage improving and / or translation-enhancing nucleic acid sequences, nucleic acid constructs comprising such sequences, and host cells comprising such nucleic acid constructs. The invention further pertains to a method for expressing a protein of interest in a cell or organism using such nucleic acid sequences, as well as their uses for increasing integration of such nucleic acid construct into a genome, for enhancing mRNA usage and / or translation of a recombinantly expressed polypeptide, and for increasing the number of transformants upon transformation of a cell with such nucleic acid construct.

Disclosed is an isolated or purified T cell receptor (TCR), wherein the TCR has antigenic specificity for a mutated human RAS amino acid sequence with a substitution of (i) glycine at position 13 with aspartic acid or (ii) glutamine at position 61 with arginine. Related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions are also provided. Also disclosed are methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal.

The present invention provides a fusion protein and an expression vector for enhancing the soluble expression of norovirus antigens. More specifically, it provides a combination of diverse genotypes of norovirus antigen proteins and RID mutations that, when norovirus antigens are used as target proteins, fold most efficiently during the formation of recombinant fusion proteins, improve the soluble expression yield of the produced fusion proteins, and enhance the assembly efficacy and homogeneity of norovirus VLPs. Furthermore, by utilizing mutational RIDs, the present invention provides a method for rapidly and in large quantities producing not only GII.4 but also other diverse genotypes of norovirus VLPs in E. coli, thereby enabling the development of VLP-based multivalent vaccines containing a wider range of genotypes.

This invention discloses a recombinant vector, recombinant bacteria, and fermentation method for high-yield GHK production, belonging to the field of biomedical technology. The recombinant expression vector of this application comprises a bicistronic expression cassette controlled by a T7 / lac heterozygous promoter, sequentially containing the encoding (GHK). n The vector encodes the first cistron of the polypeptide and the second cistron, where n is an integer from 2 to 200. When this vector is transformed into *E. coli* BL21, the resulting recombinant bacteria can simultaneously express (GHK) after IPTG induction. n Polypeptides and trypsinogen. During fermentation, trypsinogen is activated and enables the processing of (GHK). n In situ enzymatic cleavage of peptides efficiently releases GHK. This invention integrates the traditional multi-step process into a "one-step fermentation," simplifying the production process and reducing purification difficulty. Examples show that the GHK yield can reach up to 3.9 g / L, providing an efficient and convenient new strategy for the large-scale biomanufacturing of GHK.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an acid-resistant lactobacillus plantarum engineering bacterium as well as a construction method and application thereof. The recombinant lactobacillus plantarum WCFS1-gadA-speA is obtained by constructing a recombinant expression vector pMG36e-gadA-speA and inducing the expression of two key genes, and the recombinant lactobacillus plantarum WCFS1-gadA-speA is obtained. The analysis of the fermentation capacity of the strain finds that compared with a single gene heterologous expression strain, the recombinant lactobacillus plantarum WCFS1-gadA-speA shows higher acid resistance and apple juice fermentation capacity. The recombinant lactobacillus plantarum WCFS1-gadA-speA with higher strong acid tolerance is obtained through a heterologous expression technology, a strain resource with better performance is provided for the food fermentation industry, and the quality and stability of a fermented product are improved.

This invention provides an anti-CD16 antibody, the amino acid sequence of which is selected from SEQ ID NO. 1-3 in the heavy chain CDR region and from SEQ ID NO. 4-6 in the light chain CDR region. This invention belongs to the field of antibodies. It provides a protein sequence of an anti-human CD16 mouse monoclonal antibody, and designs a gene expression base sequence based on the sequenced protein sequence. A stable CHO cell line for recombinant expression of this gene is established. The purified antibody can specifically recognize the human CD16 antigen, and the antibody expression yield is much higher than that of antibodies prepared using traditional hybridoma cells. Furthermore, by replacing mouse IgG1 with rabbit IgG while retaining the variable region, a stable CHO cell line for recombinant expression of the rabbit monoclonal antibody is established. The purified antibody maintains its specific recognition of the human CD16 antigen.

This application relates to the field of maize technology, specifically to the ZmPAR1 gene and its applications. A gene encoding a nucleoside kinase was discovered in the maize genome. This nucleoside kinase was obtained through in vitro recombinant expression of the encoded gene. Testing revealed that this nucleoside kinase exhibits binding activity to various nucleoside substrates and can catalyze the synthesis of monophosphate nucleotides from nucleosides. This gene influences maize plant height, leaf angle, ear height, leaf length, and leaf width. Knocking down or overexpressing this gene can yield new maize materials, providing technical support for the breeding of new maize materials and the construction of germplasm resources.

The invention discloses an RNAi (Ribonucleic Acid Interfere) target gene for efficiently killing water borers and application of the RNAi target gene. According to the application, a recombinant expression vector capable of expressing and targeting dsRNA of the gene and recombinant escherichia coli containing the recombinant expression vector are constructed based on a Br-C gene sequence of the water borers. The dsRNA aiming at the target gene of the chilona arenaria is expressed in Escherichia coli, RNAi reaction in the chilona arenaria is induced after feeding, the target gene is down-regulated, so that the high fatality rate of the chilona arenaria can be achieved, the insecticidal method is environment-friendly and low in price, the problem that the chilona arenaria is lack of insecticidal dsRNA at home and abroad at present is solved, and the method has a wide application prospect. The method can be subsequently used for research and development of novel insecticides.

The invention belongs to the technical field of bioengineering, and particularly relates to a kluyveromyces marxianus engineering strain for recombinant expression of hydroxylated human III-type collagen and application of the kluyveromyces marxianus engineering strain. The Kluyveromyces marxianus engineering strain of the human type III collagen is obtained by carrying out recombinant expression construction on proline hydroxylase and a human type III collagen gene in the Kluyveromyces marxianus; according to the invention, two truncation designs and codon optimization are carried out on the existing natural human III type collagen amino acid sequence to obtain the human III type collagen C3S1 and C3S2, and the recombinant secretory expression in the kluyveromyces marxianus engineering strain is realized. Like hydroxylation modification of the human III type collagen, the human III type collagen C3S1 and the human III type collagen C3S2 which are subjected to recombinant expression have proline hydroxylation characteristics. The recombinant human III-type collagen is prepared by using the kluyveromyces marxianus engineering strain, the method is simple, the production efficiency is high, and large-scale production is easy.

The invention provides phospholipase B from aspergillus fumigatus and a preparation method thereof. The amino acid sequence of the enzyme is as shown in SEQ ID NO: 1, and a catalytic triad of the enzyme is composed of Ser-122, Asp-178 and His-211. The invention provides a coding gene (SEQ ID NO: 2) of the gene, an expression vector pGEX-6P-1 containing the gene, a host cell escherichia coli BL21 (DE3), and a corresponding recombinant expression and purification method. The enzyme can specifically hydrolyze sn-1 and sn-2 fatty acid ester bonds of a GPI anchor, so that GPI-APs on a membrane can be efficiently released. Compared with common PI-PLC, the PlbH has the remarkable advantages that the PlbH is more excellent in enzymatic property, more stable in effect, efficient in release capacity for various GPI-APs and the like, and a brand new tool enzyme which can replace PI-PLC and is complementary in action mechanism is provided for GPI-APs research.

The application belongs to the field of plant genetic engineering, and particularly relates to application of a rice-derived OsDREB1 gene in enhancing the low-phosphorus stress tolerance of soybeans. A recombinant expression vector of the OsDREB1 gene is constructed, and the soybean plants are transformed by using an agrobacterium-mediated method to obtain a transgenic soybean line with stable heredity. It is found through experiments that overexpression of the OsDREB1 gene in soybeans can enhance the low-phosphorus tolerance of the soybeans, and under the same conditions, the number of pods per plant and the weight of 100 seeds of the transgenic plants are higher than those of a wild control group. The growth performance of soybeans in low-phosphorus soil is improved by using genetic engineering technology, and a new strategy for reducing the dependence of agriculture on phosphorus fertilizer is provided.

The invention discloses a crassostrea hongkongensis NKA-alpha protein polyclonal antibody as well as a preparation method and application thereof. The preparation method comprises the following steps: constructing a recombinant expression vector containing an NKA-alpha target gene segment; transforming the recombinant expression vector into competent cells to construct a recombinant expression strain, inducing the recombinant expression strain to express fusion protein, and collecting and purifying to obtain NKA-alpha recombinant protein; carrying out animal immune treatment by taking the NKA-alpha recombinant protein as an antigen, collecting and separating antiserum, and purifying to obtain the Crassostrea hongkongensis NKA-alpha protein polyclonal antibody. The prepared Crassostrea hongkongensis NKA-alpha polyclonal antibody is high in affinity, can specifically recognize NKA-alpha protein in Crassostrea hongkongensis, and can be widely applied to Crassostrea hongkongensis biological immunodetection.

The application discloses a high-stability horseradish peroxidase, a recombinant expression vector, a preparation method and application. The amino acid sequence of the horseradish peroxidase is shown as SEQ ID NO. 1. Compared with wild-type horseradish peroxidase, the enzyme activity of the horseradish peroxidase is increased by 3.2 times, and compared with commercially available horseradish peroxidase, the thermal stability is better. The method can produce the horseradish peroxidase by using a tobacco transient expression system. Compared with an Escherichia coli expression system and a yeast expression system, the tobacco transient expression system can correctly fold and glycosylate, and has higher enzyme activity and stability. The purity of the horseradish peroxidase obtained according to the method can reach RZ 3.2, the enzyme activity can reach 275 U / mg, and the horseradish peroxidase can be applied to immunological detection.

The invention discloses bacillus subtilis for recombinant expression of a hermetia illucens HIAMP0438 gene and a construction method of the bacillus subtilis, and belongs to the technical field of gene engineering. The hermetia illucens recombinant protein used in the invention is obtained by expression of bacillus subtilis WB800N transferred into a pHT43-HIAMP0438 high-efficiency expression vector, and the pHT43-HIAMP0438 high-efficiency expression vector is obtained by a homologous recombination technology, so that high-level expression of the HIAMP0438 gene is realized, the HIAMP0438 gene can be efficiently secreted out of cells, the preparation process of the recombinant protein is greatly simplified, and the production cost is reduced. According to the present invention, the recombinant protein can significantly inhibit the growth of Escherichia coli, Salmonella and Listeria monocytogenes, and the recombinant protein can be used as the animal health product, and can be used for preventing and treating pig and chicken intestinal diarrhea caused by Escherichia coli and Salmonella and food-borne digestive tract diseases caused by Listeria monocytogenes.

The invention relates to the technical field of aquatic animal immune regulation and control, in particular to rHoLL-like stepped lectin-like recombinant protein as well as a preparation method and application thereof. The recombinant protein has pathogen-related molecular pattern recognition and combination capabilities, can promote aggregation of pathogens in a body fluid environment, and activates an aquatic animal complement system, so that the non-specific immune defense capability is enhanced. The preparation method comprises the following steps: amplifying a HoLL-like mature peptide coding sequence, constructing a recombinant expression vector, and converting the recombinant expression vector into an escherichia coli expression host for induced expression to obtain rHoLL-like lectin-like recombinant protein existing in an inclusion body form; and carrying out affinity chromatography purification on the inclusion body protein, and carrying out renaturation treatment in a manner of gradient reduction of denaturant concentration to obtain the rHoLL-like stepped lectin-like recombinant protein. The rHoLL-like stepped lectin-like recombinant protein has good biological safety, and can be applied to aquaculture as an aquatic animal immunopotentiator or a related biological product.

The invention discloses a method for improving salt resistance of plants. According to the method, an LSU3 gene coding sequence shown in SEQ ID NO: 1 is introduced into a plant cell and an LSU3 protein is expressed, so that the viability and the growth condition of the plant in a salt stress environment are remarkably enhanced. The invention further provides a recombinant expression vector containing the gene, a construction method of the recombinant expression vector and a specific application scheme of the recombinant expression vector in improvement of the salt resistance of arabidopsis thaliana, and effective gene resources and technical means are provided for salt-resistant breeding of crops.

The invention belongs to the technical field of synthetic biology and enzyme engineering, and particularly relates to a multi-enzyme in-vivo space co-localization carbon metabolic flux strengthening method, a genetically engineered bacterium and application of the genetically engineered bacterium. According to the invention, a recombinant expression vector is constructed, key enzymes RHLA and RHLB participating in synthesis of a target product are respectively subjected to fusion expression with protein scaffold modules RIAD and RIDD through (G4S) 3 flexible connecting peptide, and are introduced into host chassis bacteria, so that a stable multi-enzyme composite system is formed in cells, the transmission distance of a metabolic intermediate is shortened, and the expression efficiency is improved. Spatial co-localization and concerted catalysis of key catalytic enzymes are realized, and the conversion efficiency of a carbon source to a target product is improved. Compared with the prior art, the genetically engineered bacterium constructed by the invention has the advantages of stable structure, no need of complex regulation, reasonable metabolic flow distribution and the like in the fermentation process, the synthesis efficiency and yield of the target biosurfactant are remarkably improved, and the feasibility of industrial amplification application is enhanced.