56 results about "Sterile filtration" patented technology

The invention discloses a preparation method of honey kangpu tea based on pure bacterium synergistic fermentation, and relates to the field of tea preparation. Comprising the following steps: culturing strains, namely culturing koal fotamentosa, zygosaccharomyces bayer and lactobacillus plantarum; black tea is added into boiling water to be soaked, sterilization is performed after filtering, and acacia honey is added after cooling to be evenly mixed; fermenting, namely inoculating the fermentation substrate with the foal foal bacillus, the zygosaccharomyces bayer and the lactobacillus plantarum, sealing with gauze, and carrying out aerobic fermentation; sterilizing and filling: after fermenting and planting, immediately performing sterile filtration or clear liquid, and then filling after pasteurization. Fermentation can be completed in 14 days through synergistic metabolism of three bacteria, the fermentation efficiency is improved, the fermentation period is shortened, unique sweet and sour balance is formed through synergistic effect of acetic acid and lactic acid, meanwhile, pure bacteria are used for fermentation, infectious microbe pollution is avoided in the fermentation process, and harmful metabolites such as aflatoxin in the honey kangpu tea are avoided.

Sterile filtered liquid lactase composition comprising a lactase, preferably a neutral or acidic lactase, an anion selected from malate, tartrate, citrate, gluconate and / or EDTA and a cation selected from sodium and potassium. Preferably, the composition further comprises a polyol. Addition of the said anions to lactase improves filterability by reducing pressure in a filtration system. Preferably the filtration forms part of an in-line filtration system in the production of a dairy product.

The invention relates to the field of devices for microbiology, in particular to an intelligent constant-pressure constant-temperature sterile air system for liquid fermentation of edible and medicinal fungus hyphae. A tail gas outlet is formed in the inner tank; an inlet and an outlet are formed in the outer tank; the tail gas outlet is communicated with a heat source inlet of a heat exchanger, a heated gas inlet of the heat exchanger is communicated with a compressed air pipeline used for conveying compressed air, and a heated gas outlet of the heat exchanger is communicated with an air inlet of a filtering assembly used for conducting sterile filtering on the compressed air. An air outlet of the filtering assembly is communicated with an air inlet of the inner tank, and the air inlet is formed in the bottom of the inner tank. The problem that an existing edible and medicinal fungus hypha liquid fermentation system is unstable in hypha culture effect is solved, and the intelligent constant-pressure and constant-temperature sterile air system for edible and medicinal fungus hypha liquid fermentation can enable the hypha culture effect to be stable.

The application provides a preparation process of a sterile compound cinnabar freeze-dried stock solution and relates to the technical field of traditional Chinese medicine deep processing. The preparation process comprises the following steps: S1: taking compound cinnabar liquid alcohol precipitation paste, adding purified water to a certain volume, and cold storage and standing to obtain a mixed solution; S2: taking supernatant of the mixed solution in step S1, performing sterile filtration at least i times through a filter membrane at a temperature of 28-45 DEG C to obtain a sterile compound cinnabar freeze-dried stock solution. In the application, i is a natural number and traverses from 1 to n, n is a natural number and is not less than 3. The pore size of the filter membrane for the (i+1)th filtration is not greater than the pore size of the filter membrane for the ith filtration; and the certain volume in step S1 is configured to be 580-1000 ml. The sterile compound cinnabar freeze-dried stock solution prepared by the application can reduce the volume of the sterile compound cinnabar freeze-dried stock solution while ensuring that the material basis does not change significantly through the improvement of the processing technology.

Method for performing a filter test, wherein a) a plurality of hoses (5) are or are laid from a first cleanroom (17.1) of a first, higher cleanroom class to a second cleanroom (17.2) of a second, lower cleanroom class, wherein a sterile filter set (1) is provided in the first cleanroom (17.1), wherein at least one fluid container (19), at least one drain container (21) and a filter test device (23) are provided in the second cleanroom (17.2), wherein b) at least one first hose (5.1) of the plurality of hoses (5) is or becomes fluidically connected to the sterile filter set (1) with at least one first end, wherein c) the at least one first hose (5.1) of the plurality of hoses (5) is or becomes fluidically connected to the at least one fluid container (19) with at least one second end, wherein d) at least one second hose (5.2) of the plurality of hoses (5) is or becomes fluidically connected to the sterile filter set (1) with at least one first end, wherein the at least one second hose (5.2) of the plurality of hoses (5) opens into the at least one drain container (21) with at least one second end, wherein e) the filter set (1) is rinsed by passing a rinsing fluid from the at least one fluid container (19) through the filter set (1) into the at least one drain container (21), wherein f) after rinsing the filter set (1) the second end of the at least one first hose (5.1) is separated from the fluid container (19) and connected to the filter test device (23) in a flow-related manner, wherein g) a filter test is carried out using the filter test device (23).

The utility model relates to the technical field of corrugated board processing, and discloses an air compressor for adding sterile air, which comprises an air compressor main body, two connecting frames are fixedly mounted at the top of the air compressor main body, the air compressor main body is connected with an air compression bin through the two connecting frames, the air compression bin is communicated with the inside of the air compressor main body, and the air compression bin is connected with the air compressor main body. An air inlet pipe is fixedly connected to the interior of the front portion of the air compressor body in a penetrating mode, an air outlet pipe is fixedly connected to the interior of the rear portion of the air compressor body in a penetrating mode, a sterile filter cartridge is placed in the air inlet pipe, a filter cover is installed on the portion, located in the air inlet pipe, of the front portion of the sterile filter cartridge, and a connecting rod is fixedly connected to the front portion of the filter cover. According to the air compressor, air entering the air compressor body is sterile air, then pressurized air is also in a sterile state, and the situation that the air compressor fails to process food due to the fact that the pressurized air contains bacteria in the food processing process is avoided.

The invention belongs to the technical field of medicine, and discloses an artemisia annua pollen allergen sublingual drop as well as a preparation process and application thereof.The preparation is realized through the following core processes: firstly, performing raw material screening and supercritical CO2 degreasing pretreatment on artemisia annua pollen at the full-bloom stage in North China, then performing graded ultralow-temperature freezing and crushing, and performing freeze drying to obtain the artemisia annua pollen allergen sublingual drop. The particle size D90 of the superfine powder is smaller than or equal to 4 microns; then adopting a neutral protease and pectinase composite enzymatic hydrolysis system, and combining 4 DEG C low-temperature pulse ultrasound-assisted treatment to accelerate the dissolution of allergens; carrying out tangential flow grading purification and buffer solution replacement by virtue of 100kDa and 5kDa PES (Polyether Sulfonate) ultrafiltration membranes, and introducing a compound stabilizer; and finally, performing three-stage sterile filtration, and performing encapsulation and preservation. According to the process, through multi-link collaborative optimization, the extraction efficiency and activity stability of allergens are improved, and the prepared sublingual drops have the effects of improving allergic rhinitis, allergic asthma and airway inflammation and also have excellent permeability.

The application provides a preparation method and application of a sterile inhalation suspension containing a PDE-4 or PDE-3 / 4 inhibitor. The preparation method comprises dispersing the PDE-4 or PDE-3 / 4 inhibitor in injection water containing an excipient, high-pressure wet heat sterilization to obtain a PDE-4 or PDE-3 / 4 inhibitor dispersion; further comprising dissolving a long-acting beta2-receptor agonist and / or a long-acting muscarinic receptor antagonist, sterile filtration to obtain a mixed solution; mixing the PDE-4 or PDE-3 / 4 inhibitor dispersion with the mixed solution, dispersing and sterilely filling. The pharmaceutical preparation prepared by the application is a sterile inhalation suspension, the sterile inhalation suspension containing the PDE-4 or PDE-3 / 4 inhibitor has high bioavailability, good patient medication compliance and good preparation stability.

The invention relates to the technical field of biology, in particular to a moisturizing and whitening composition containing stem cell exosomes as well as a preparation method and application of the moisturizing and whitening composition. According to the composition, adipose-derived mesenchymal stem cell exosomes are combined with two specific modified compounds to form a stable and efficient oil-in-water emulsion. The preparation method comprises the following steps: culturing stem cells under a low-oxygen condition, and separating exosomes through differential ultracentrifugation; the preparation method comprises the following steps: respectively preparing a water phase mainly containing vanillin-derived dynamic imine bond grafted hyaluronic acid and an oil phase mainly containing disulfide bond crosslinked polyester vitreous network powder; adding exosome and other active ingredients after two-phase emulsification, and finally performing sterile filtration to obtain a finished product. The composition makes full use of the repair and regeneration capacity of the exosome and the synergistic interaction of the two modified compounds, has excellent moisturizing, whitening and anti-aging effects, and is suitable for preparation of various cosmetics.

The present application relates to the field of device for microbiology, specifically relates to edible and medicinal fungus mycelium liquid fermentation intelligent constant pressure constant temperature sterile air system, the system includes fermentation tank;The tail gas outlet is opened on the inner tank, the inlet and outlet are opened on the outer tank;The tail gas outlet is communicated with the heat source inlet of heat exchanger, the heated gas inlet of heat exchanger is communicated with compressed air pipeline for conveying compressed air, the heated gas outlet of heat exchanger is communicated with the air inlet of filter assembly for sterile filtering compressed air, the air outlet of filter assembly is communicated with the air inlet of inner tank, and the air inlet is located at the bottom of inner tank.The existing edible and medicinal fungus mycelium liquid fermentation system has the problem of unstable mycelium culture effect, and the edible and medicinal fungus mycelium liquid fermentation intelligent constant pressure constant temperature sterile air system can make the mycelium culture effect more stable.

The present invention relates to a method for the sterile filtration of an aqueous albumin-containing solution (2), where the method comprises filtering the solution (2) with at least one filter (3) to obtain a filtrate (1), where the filtrate (1) is filled in at least one container (10, 10 ') before the filtrate (1) is filled in the at least one container (10, 10'), the at least one container (10, 10 ') is filled in the at least one container (10, 10'). The at least one filter (3) is conditioned by washing the at least one filter (3) with an amount (5) of the albumin-containing aqueous solution (2) under the following conditions: i) at a differential pressure across the respective filter (3) of at most 1 bar and / or ii) by means of pressure filtration, wherein the filtrate (1) is maintained at a back pressure of at least 0.5 bar in the outlet (4) of the respective filter (3).

The application discloses a high-stability and low-irritation oxytetracycline long-acting injection and a preparation method thereof. 2+ EDTA-2Na and sodium formaldehyde bisulfite are coordinated to stabilize, and forsythia extract reduces irritation; the pH of the finished product is 8.2-8.8. The method comprises acid pre-coordination, pre-filtration, ultrafiltration, reduction and deoxygenation (dissolved oxygen is less than or equal to 2 ppm) and 0.22 mu m terminal sterile filtration. The application realizes long-term clarification and content stability under high loading, and inhibits metal catalytic oxidation and alkali-sensitive degradation.

The utility model discloses an improved enzyme preparation sterile filtering device, which relates to the field of enzyme preparation filtering, and comprises a fixing frame, a filtering structure is fixedly connected in the fixing frame, the filtering structure comprises two groups of single-group filter cartridges, and the number of the single-group filter cartridges is two. In the preparation and purification process of the enzyme preparation, the device can meet filtering operation with different precision requirements, the core is a unique filtering structure, the device has flexible layered filtering capacity, and the filtering level can be easily adjusted according to the actual production requirement so as to realize accurate purification of the enzyme preparation. In addition, an extra filter material is arranged in the liquid outlet pipeline in the device, a user only needs to simply screw a valve in the pipeline, the filtering speed and the fine degree can be regulated and controlled at will, the design is easy and convenient to operate, the filtering efficiency and flexibility are greatly improved, and powerful support is provided for industrial production of enzyme preparations.

A method is provided for producing a composition that includes a nanoparticle with at least one nucleic acid and at least one ionizable lipid. The method includes introducing a first composition that includes the at least one nucleic acid and a second composition that includes the at least one ionizable lipid into at least one reactor. The first composition and the second composition are introduced at a first flow rate and a second flow rate, respectively, therefore generating the nanoparticle in a reaction mixture. The method further includes filtering the nanoparticle from the reaction mixture via a single-pass tangential flow filter at a feed flux to provide a retentate, to produce the composition. The method may also include filtering the retentate through a sterile filtration membrane.

The utility model discloses a fat filtering collector, and particularly relates to the technical field of fat filtering, the fat filtering collector sequentially comprises a filtering assembly, a waste liquid collecting assembly and a sterilizing assembly from top to bottom, the fat filtering collector is used for aseptically filtering fat, an auxiliary assembly used for improving the filtering efficiency is arranged on the outer side of the filtering assembly, and the filtering assembly comprises a filtering cylinder. A first bottom plate is fixed to the bottom of the inner wall of the filter cylinder, a filter screen for filtering fat is fixed to the inner wall of the filter cylinder, the waste liquid collecting assembly comprises a waste liquid cylinder, a top plate is fixed to the top end of the inner wall of the waste liquid cylinder, and a second bottom plate is fixed to the bottom of the inner wall of the waste liquid cylinder; and the sterilization assembly comprises a sterilization cylinder and an ultraviolet sterilization lamp fixed at the bottom end in the sterilization cylinder. The interior of the collector can be sterilized at any time in the whole fat filtering process, whole-process sterile filtering and storage of fat are achieved, the fat purity is improved, and therefore the survival rate and the effect after fat transplantation are improved.

The utility model discloses an on-line sterile filter, which relates to the field of on-line sterile filtration, and comprises a filter outer pipe, a sterile filtration component is arranged in the filter outer pipe, a feeding and discharging component is arranged on the periphery of the filter outer pipe, and a material monitoring component is arranged on one side of the sterile filtration component. And the processing technology is not complicated. Different specifications of the filter can be increased according to different requirements and the filtering area. Application is convenient, and production efficiency is greatly improved. The utility model is suitable for fluid food sterilization machines.

The invention provides a phosphorus-knowing microorganism enrichment culture and terrace layout integrated device, which relates to the technical field of agricultural microorganisms and comprises a selective enrichment culture reactor, a bacterium hanging culture unit and a one-way connecting channel. The enrichment culture reactor is composed of a reactor shell and a top cover, a stirring device and an indissolvable phosphorus particle bed box supported by a supporting plate are arranged in the enrichment culture reactor, an aeration air inlet is formed for introducing sterile air / oxygen, an exhaust port is connected with a sterile filter, and pH, temperature and dissolved oxygen probes can be arranged. The hanging bacteria culture unit is a detachable container, is internally provided with a hollow porous carrier cylinder which is detachably connected with a top cover of the hanging bacteria culture unit, and is provided with a liquid supplementing port and an exhaust port. The one-way connecting channel is composed of a sterile isolating valve, a one-way valve and a quick connector, and a pump body can be arranged according to needs to assist in conveying the culture solution. According to the device, selective enrichment and carrier bacterium hanging can be realized under the closed sterile condition, the pollution risk is reduced, and the culture efficiency and the arrangement convenience are improved.

The present invention provides a method for preparing a phenol red-containing trypsin solution with uniform color distribution after cryopreservation, comprising the following steps: weighing buffer components and phenol red, mixing them, and ball-milling to obtain a pretreated buffer component powder; weighing the pretreated buffer component powder, trypsin powder, and ultrapure water, mixing them, stirring, and performing sterile filtration to obtain the phenol red-containing trypsin solution; wherein the passing rate of the pretreated buffer component powder through a 100-mesh sieve is ≥95%. By determining reasonable grinding conditions and adopting the method of grinding first and then dissolving, the present invention makes the material particle size uniform, the particle size small, and the dissolution speed faster, and it is easier to form a uniform solution during the dissolution and stirring process, ensuring that the prepared phenol red-containing trypsin solution remains a uniform light yellow color after being cryopreserved at -20°C for 24 hours.

The utility model provides an ultrafiltration concentration tube device with a sterile filtration function, which solves the problems that the existing ultrafiltration tubes with different specifications and models can only achieve the purpose of protein concentration, different proteins can be confused due to frequent operation after the concentration is finished, the recovery rate is reduced, the risk of introducing bacteria is increased and the like. According to the main scheme, a tube body internally provided with a cavity is included, the top of the tube body is open, the tube body can be sealed into a whole through a tube cover, the tube body is formed by splicing three sections, the adjacent splicing positions are fixed through deformation friction, and the three sections of the tube body sequentially comprise an antibody storage area, a liquid storage area and a liquid discharging area from the upper end opening to the lower end opening; a first supporting table and a second supporting table are fixed to the two sequentially-connected positions of the inner wall of the pipe body respectively, a sterile filter membrane is embedded in the first supporting table, and an ultrafiltration membrane is embedded in the second supporting table.

The utility model relates to the technical field of fermentation, and discloses an aeration type thermophilic microbial agent domestication / propagation system which comprises a heat preservation box (1), an air compressor (2), a flow meter (3), a fermentation tank (4) and an aeration stone (5), wherein the heat preservation box (1) is connected with the air compressor (2), and the air compressor (2) is connected with the flow meter (3); the heat preservation box (1) comprises a box body (11), a gas channel (12), a cavity (13), a support (14) and a temperature control device. The support (14) is arranged in the cavity (13) and comprises a support frame and support strips, the support strips are installed on the support frame in parallel, and the fermentation tank (4) is arranged between every two adjacent support strips; the fermentation tank (4) is also connected with the flow meter (3); the system further comprises a sterile filter (6), and the sterile filter (6) is connected with the heat preservation box (1). The aeration type thermophilic microbial agent domestication / expanding culture system provided by the utility model can be used for performing domestication / expanding culture research on microorganisms under high-temperature sterile conditions.

The utility model relates to a kind of multifunctional sterile feeding, taking material shake flask, including bottle body and bottle plug, the bottle body upper portion is fixedly connected feeding pipe and taking material pipe;The one end of feeding pipe is located in bottle body and is equipped with feeding diffuser, the other end is located outside bottle body and is sequentially equipped with first water stop clamp and sterile filter;The sterile filter is connected feeding syringe;The one end of taking material pipe extends to bottle body bottom along the inner side wall of bottle body, the other end is located outside bottle body and is sequentially equipped with second water stop clamp and third water stop clamp.The utility model is fixed with the feeding pipe and taking material pipe of water stop clamp by being set on bottle body, to facilitate feeding operation and taking material operation in bottle body without opening bottle;Feeding pipe of the utility model adopts feeding diffuser to feed in bottle body middle part to ensure uniform feeding each time, prevent material liquid local over-acid over-alkali in feeding process;Sterile filter is used to sterilize and filter feeding, further reduce the risk of bottle contamination.

The invention relates to the technical field of medical instruments, in particular to a liquid food drinking device for critical patients, which is characterized in that open sliding grooves are formed in the inner walls of the two sides of the upper end of an open tank, sliding blocks are slidably connected in the open sliding grooves, the opposite sides of the sliding blocks are fixedly connected with a sterile filter plate, and the middle of the sterile filter plate is rotatably connected with a rotating rod through a bearing; a clamping groove is formed in the upper end of the rotating rod, supporting rods are fixedly connected to the rod walls of the two sides of the lower end of the rotating rod, scraping plates are fixedly connected to the opposite sides of the supporting rods, and sliding grooves are formed in the rod walls of the other two sides of the rotating rod. The deterioration risk caused by temperature change is reduced; meanwhile, through the slidable sterile filter plate, liquid food entering the device can be filtered, particle impurities or foreign matter can be intercepted, the possibility of infection and obstruction of the digestive tract of a patient is reduced, sterility and safety of liquid food supply are guaranteed, and disassembly and washing in the later period are convenient.

The present invention relates to a filtration system for sterile filtration of particles having a hydrodynamic diameter of at most 400 nm as well as a method for sterile filtration of particles having a hydrodynamic diameter of at most 400 nm, both of which achieve a high yield of target molecules and sterility.

To make it possible to continuously produce sterile water, in a sterile water producing system using a sterile filter.SOLUTION: RO water is supplied to the liquid feed line 22A of the sterile water production system 10 through pump 12. The sterile water production system 10 is equipped, in this order from the upstream side, with a first sterile filter 22A for sterilizing the RO water, second sterile filters 18A and 18B provided in parallel and switchable, and a UV sterilizer 20 that irradiates the water with ultraviolet light to sterilize it. A sampling line 40 branches off from the upstream-side liquid feed line of the UV sterilizer 20, and the number of microorganisms in the sampled water is detected by a microorganism monitoring device 44. During the production of sterile water, if the number of microorganisms detected by the microorganism monitoring device 44 is equal to or greater than a predetermined value, the flow path passing between the parallel second sterile filters 18A and 18B is switched.SELECTED DRAWING: Figure 1

The invention discloses a sterile filtering device for tissue stem cells, and belongs to the technical field of sterile filtration, the sterile filtering device comprises a filter kettle, a plurality of filter elements are arranged in the filter kettle in a surrounding manner, the bottom side of the filter kettle is connected with a negative pressure suction pipe and a backwashing pipe, and the sterile filtering device further comprises a material distribution disc connected to the top of the filter kettle and located on the bottom side of a feeding pipe of the filter kettle. According to the tissue stem cell liquid filtering device, tissue stem cell liquid to be filtered is fed from the top of the filtering kettle, large-particle impurities of the tissue stem cell liquid can be filtered through the filter element arranged in a surrounding mode, the tissue stem cell liquid can be intermittently pumped into the filter element through the designed liquid separation mechanism, the filtering load of the filter element is reduced through intermittent feeding, the filtering precision is improved, and the filtering efficiency is improved. The feeding impact efficiency of the tissue stem cell liquid is improved through the liquid separation mechanism, and the filtering contact efficiency with the filter element is improved.

The invention provides a refining method of a light dimeticone pharmaceutic adjuvant, and relates to the field of pharmacy. The refining method comprises the following steps: heating a dimethyl cyclosiloxane crude product to a first temperature, stirring, and removing low-boiling-point substances under a first negative pressure condition to obtain a refined solution; mixing the refined liquid with a weakly alkaline aqueous solution, stirring, standing and separating liquid to obtain an upper layer oily substance; heating the upper-layer oily substance to a second temperature, introducing water vapor under normal pressure, carrying out first water vapor distillation, discarding a distillation light liquid, and carrying out second water vapor distillation on the distillation substrate under a second negative pressure condition to obtain a distillation light liquid; and separating the distilled light liquid, removing a water layer, drying an oil layer under reduced pressure, and sterilizing and filtering to obtain a medicinal-grade cyclomethylsilicone product. According to the refining method provided by the invention, the current situation that the medicinal cyclomethylsilicone is single in component, many in impurities and poor in emulsification and lubrication effects in a medicinal preparation is solved, the application effect of the preparation is effectively improved, and the problem that the cyclomethylsilicone cannot meet the medicinal requirement is solved.

The invention discloses a low-temperature sterile filtration edible and medicinal fungus extraction device and method, and relates to the technical field of edible and medicinal fungus extraction.The low-temperature sterile filtration edible and medicinal fungus extraction device comprises a machine shell, a low-temperature sterile tank is fixedly installed on the top face of the machine shell, a tank cover is fixedly installed on the top face of the low-temperature sterile tank, and a valve is installed in the middle of the bottom face of the low-temperature sterile tank in a penetrating mode; the end, away from the low-temperature sterile tank, of the valve penetrates through and is fixed to the top face of the machine shell, the square cover is installed on the right side of the machine shell in a sealed mode, the liquid outlet pipe penetrates through and is fixed to the bottom of the left side of the machine shell, the liquid outlet pipe is used for discharging medicine liquid, and the exhaust pipe penetrates through and is fixedly installed on the left side of the top face of the machine shell. The wall face of the machine shell is scraped clean through the chamfers of the square plate, and therefore the problem that the medicine residue squeezing and filtering effect is poor due to the fact that medicine residues of edible and medicinal fungi can be attached to the wall face of the machine shell is solved.

The invention discloses injectable temperature-sensitive hydrogel for oral mucosa protection and a preparation method of the injectable temperature-sensitive hydrogel, and relates to the field of biological materials.The preparation method comprises the following steps that poloxamer F127 and gelatin are added into deionized water and stirred until the poloxamer F127 and the gelatin are completely dissolved, and transparent sol is formed; slowly dropwise adding an APBA solution into the transparent sol at 0-4 DEG C, and stirring to form a reversible borate bond network and construct a dynamic cross-linking system; adding AA2G into the dynamic cross-linking system, stirring and mixing in a dark place, carrying out ultrasonic dispersion to completely dissolve and disperse the AA2G into the dynamic cross-linking system, finally adding QCS into the reaction system, stirring and mixing for 30 minutes at 100 rpm in a dark place, and carrying out ultrasonic dispersion; and heating to 37 DEG C, adjusting the pH value to be neutral, carrying out sterile filtration, and subpackaging to obtain the temperature-sensitive hydrogel.

The utility model provides an upright adapter for a disposable sterile filter, and belongs to the technical field of sterile filters. The disposable sterile filter upright adapter comprises a supporting assembly and a connecting assembly, the supporting assembly comprises a bottom plate and a supporting piece, the supporting piece is arranged at the top of the bottom plate, the connecting assembly comprises a top plate, a connecting rod, a disposable sterile filter body and a limiting plate, the connecting rod is arranged at the bottom of the top plate, and the limiting plate is arranged on the connecting rod. By arranging the bottom plate, the supporting piece, the top plate, the connecting rod, the disposable sterile filter body and the limiting plate and forming the bayonets, stable support is provided by utilizing the bottom plate, the supporting piece and the top plate, the top plate with different bayonets can be replaced, and the disposable sterile filter body is butted by virtue of the bayonets, so that the connecting stability is ensured; the disposable sterile filter body is stably supported to be in a vertical state, so that liquid is in full contact with a filter membrane, the filtering efficiency and the operation convenience are improved, and the precise and stable sterile filtering operation is ensured.

The invention discloses a preparation process of vitamin K1 injection mixed micelles, which belongs to the technical field of pharmaceutical preparations, and comprises the following preparation steps: under the protection of inert gas, taking part of water for injection, adding glycocholic acid, stirring and dispersing; s2, under the stirring condition, soybean lecithin and vitamin K1 in the prescription dosage are added into the solution obtained in the S2 at the same time, the stirring rotating speed is controlled to be 800-1000 rpm, and stirring is continuously conducted till the solution becomes a uniform and clear micelle solution; supplementing the water for injection to full dose, and adjusting the pH value of the system to 5.5-6.5 by using a sodium hydroxide solution; performing sterile filtration on the liquid medicine obtained in the step S4 through a 0.22 mu m filter membrane; and filling the filtered liquid medicine into a sealed container under the protection of inert gas, and carrying out terminal sterilization. The process parameters are clear and easy to control, key quality control points are clear, the process transfer and amplification process from laboratory test to industrial mass production is stable and reliable, and batch-to-batch consistency is high.