7 results about "Sterile filtration" patented technology

The application discloses a high-stability and low-irritation oxytetracycline long-acting injection and a preparation method thereof. 2+ EDTA-2Na and sodium formaldehyde bisulfite are coordinated to stabilize, and forsythia extract reduces irritation; the pH of the finished product is 8.2-8.8. The method comprises acid pre-coordination, pre-filtration, ultrafiltration, reduction and deoxygenation (dissolved oxygen is less than or equal to 2 ppm) and 0.22 mu m terminal sterile filtration. The application realizes long-term clarification and content stability under high loading, and inhibits metal catalytic oxidation and alkali-sensitive degradation.

The present invention belongs to the technical field of purification of polypeptide drugs, and specifically relates to a method for purifying tirzepatide, which method comprises: (a) preparing a crude solution of tirzepatide by using an aqueous acetonitrile solution; (b) subjecting the crude solution of tirzepatide to a first purification by using a specific stationary phase, mobile phase and elution gradient to obtain a first fraction; (c) subjecting the first fraction to a second purification by using a specific stationary phase, mobile phase and elution gradient to obtain a second fraction; and (d) subjecting the second fraction to nanofiltration concentration, salt exchange, sterile filtration and lyophilization to obtain a tirzepatide product. The product obtained by the provided purification method has a purity of over 99.5% and a total purification recovery rate of over 80%. The provided single-injection elution cycle lasts only 35-40 minutes, which greatly reduces the use of organic solvents in the purification process of tirzepatide, lowers purification costs, and promotes environmentally sustainable development.

The application belongs to the technical field of bioactive extract preparation, and discloses a preparation method of high-purity animal placenta extract with controlled enzymolysis and molecular weight, which comprises sequentially connected raw material low-temperature homogenization treatment, gradient temperature-controlled enzymolysis, transient mild enzyme inactivation, double-mode membrane fractionation for molecular weight control, low-temperature concentration, activity locking and sterile filtration. The application adopts a complex protease system matched with a segmented gradient temperature-controlled enzymolysis process, and combines a neutral mild pH environment of 6.3-7.8 to accurately control the enzymolysis site and degradation degree, completely solve the technical problems of uncontrollable enzymolysis, excessive degradation or insufficient degradation and excessive large molecule residue caused by single enzymolysis and extensive enzymolysis in the traditional process, and greatly improve the uniformity of the enzymolysis process, thereby guaranteeing the stable quality of the extract from the source and being beneficial to actual application and operation.

This utility model belongs to the field of food production technology, specifically disclosing an automatic control system for aseptic air pressure. It includes an aseptic air pipeline, a compressed air source, a first target tank, and a control device. Along the airflow direction, the aseptic air pipeline is sequentially equipped with a first pressure reducing valve, a filter assembly, a second pressure reducing valve, a third pressure reducing valve, a first aseptic filter, and a first pressure monitoring device. The compressed air source is connected to the end of the aseptic air pipeline near the first pressure reducing valve, and the first target tank is connected to the end of the aseptic air pipeline near the first pressure monitoring device. Both the first pressure monitoring device and the third pressure reducing valve are electrically connected to the control device. This utility model achieves automated control of aseptic air pressure, improves the stability of aseptic air pressure, and ensures product quality. This utility model is applicable to positive pressure protection of the target tank.

The application discloses a VHP sterilization system capable of quickly discharging residual substances, which comprises a sterilization cabin, an air supply assembly, an air exhaust assembly and a sterilization assembly. The air supply assembly comprises an air supply pipeline and an air supply high-efficiency filter, and the air supply pipeline is communicated with the inside of the sterilization cabin. The air exhaust assembly comprises an air exhaust pipeline and an air exhaust high-efficiency filter, and the air exhaust pipeline is communicated with the inside of the sterilization cabin. The sterilization assembly comprises a flash generator, a first pipeline and a sterilization high-efficiency filter. One end of the first pipeline is communicated with the flash generator, and the other end of the first pipeline is communicated with the inside of the sterilization cabin. The VHP sterilization system provided by the application filters hydrogen peroxide vaporized by the flash generator by using a separate sterilization high-efficiency filter, thereby ensuring the sterile filtration of sterilization gas. Meanwhile, the sterilization high-efficiency filter is not arranged on the air supply pipeline, thereby solving the problem that residual hydrogen peroxide on the sterilization high-efficiency filter causes the increase of the discharging time when the air supply assembly works to discharge residual substances from the sterilization cabin.

This invention discloses a method for producing Ac-225 mother liquor by irradiating a thorium target, belonging to the field of medical radioisotope production technology. Through steps such as designing a composite target of nano-scale thorium dioxide powder and high-purity niobium powder, medium-energy proton beam irradiation, high-temperature fluorination pretreatment, HDEHP extraction for impurity removal, TODGA extraction enrichment, and terminal aseptic filtration, efficient large-scale production of Ac-225 is achieved. This invention solves industry pain points such as dependence on reactors, poor target irradiation stability, low separation and purification efficiency, insufficient radiochemical purity of the product, and long production cycle of traditional processes. It achieves an Ac-225 recovery rate of 85.85%, a radiochemical purity of 99.99%, an endotoxin content of <0.25 EU / mL, and a production cycle shortened to 28 days. This invention offers controllable process parameters, high yield, high purity, and strong aseptic assurance, making it suitable for the large-scale and stable production of medical-grade Ac-225 mother liquor.

This invention discloses a high-efficiency greenhouse cultivation method for *Antrodia camphorata* in the field of medicinal fungal cultivation technology, including strain activation, substrate preparation, inoculation culture, and fruiting management. The substrate contains wheat bran, corn flour, sucrose, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, and lanthanum-modified multi-level carbon-silicon composite nanomaterials, with vitamin B1 added after sterile filtration. After static cultivation in a greenhouse, the mycelium is induced to produce fruiting bodies through atomized spraying, and then dried after harvesting. The lanthanum-modified multi-level carbon-silicon composite nanomaterials are prepared by chitosan coordinating with lanthanum nitrate, precipitating with anhydrous ethanol, assembling with mesoporous silica, cross-linking with 3-aminopropyltriethoxysilane and tetraethyl orthosilicate under glacial acetic acid catalysis, and finally activated by gradient calcination and washing with anhydrous ethanol. This invention avoids the Maillard reaction and surface crusting during high-temperature sterilization, protects the activity of the lanthanum oxide crystal phase, significantly improves the mycelial growth rate and triterpenoid yield of *Antrodia camphorata*, and shortens the cultivation cycle.