211results about How to "Avoid degradation" patented technology

According to the method, a one-pot copolymerization method is adopted, MBAA and borax serve as cross-linking agents, zwitterionic polyelectrolyte hydrogel containing SBMA, PVA, THMA and AMPS is prepared, and the zwitterionic polyelectrolyte hydrogel has excellent adhesion performance and biocompatibility and is suitable for the field of biological medicine. According to the method, the physical characteristics of the hydrogel are enhanced due to crosslinking sites and hydrogen bonds of polyvinyl alcohol. The cationic and anionic groups of SBMA improve the adhesion performance, and the reaction monomer THMA provides hydrogen-bond interaction to further promote adhesion, so that the prepared hydrogel adhesive shows excellent adhesion performance and biocompatibility, and is suitable for biological medicine application of wound dressing, drug release systems, tissue engineering and the like.

The invention belongs to the technical field of biology, and particularly relates to a reagent combination and a kit for extracting nucleic acid through a paramagnetic particle method and application of the reagent combination and the kit. The reagent combination or the kit does not adopt beta-mercaptoethanol with pungent smell or dithiothreitol needing to be stored at low temperature as an antioxidant, but adopts tris (2-formylethyl) phosphine hydrochloride and / or metabisulfite as an antioxidant, belongs to common salt chemical reagent powder, can be stored at normal temperature, and can be used for preparing a reagent for the first time. Manual addition by experimenters before an experiment is not needed, so that nucleic acid (RNA) released by splitting decomposition can be effectively prevented from being oxidized and denatured, and the integrity of extracted nucleic acid (total RNA) fragments is ensured; especially, the metabisulfite is commonly used in food additives, does not cause harm to the body, and is non-toxic and non-volatile.

The invention discloses a preparation method of a chitosan oligosaccharide-anthocyanin nanocomposite. The preparation method comprises the following steps: dissolving purple corn anthocyanin in distilled water; dissolving chitosan oligosaccharide in distilled water; the method comprises the following steps: adding an organic solvent into a reaction kettle, reacting at room temperature in a dark place for 4-48 hours, transferring a mixed solution into a centrifugal tube, centrifuging for 10-30 minutes to separate out nano-composite precipitates and uncombined supernatant, and then adding ultrapure water for repeated centrifugation. And finally, carrying out freeze drying treatment on the precipitate to obtain the final chitosan oligosaccharide-anthocyanin nano-composite. The nano-composite prepared by the method has high stability, good dispersibility and enhanced biological activity, and provides a new way for wide application of chitosan oligosaccharide and anthocyanin in the fields of food, cosmetics, biological medicine and the like.

The invention provides a PEF composite board and a preparation method, and relates to the technical field of insulation boards, the composite PEF board is formed by bonding a plurality of layers of board bodies, and comprises a base layer, the base layer is prepared from the following components in parts by weight: 58 to 60 parts of HDPE (High-Density Polyethylene), 4 to 6 parts of nano montmorillonite, 1 to 2 parts of benzoyl peroxide, 2 to 5 parts of BHT (Butylated Hydroxytoluene) heat stabilizer and antioxidant, 2 to 5 parts of maleic anhydride grafted polyethylene, 0.2 to 1 part of magnesium stearate and 0.3 to 0.8 part of epoxidized soybean oil; comprising a fireproof layer which is composed of the following components in parts by weight: 45-55 parts of HDPE and 5-15 parts of a phosphate ester environment-friendly flame retardant; comprising a thermal insulation layer and is prepared from the following components in parts by weight: 45-55 parts of HDPE, 5-10 parts of lignin fibers and 5-10 parts of a bio-based foaming agent formed by mixing sodium bicarbonate and citric acid. The invention has the beneficial effects that the mechanical strength, flame retardant property and thermal insulation effect of the PEF plate are obviously improved, the weather resistance and environmental protection property are enhanced, the optimal balance of high performance and cost effectiveness is realized, and the PEF plate is suitable for various building and industrial application environments.

The invention relates to a diatomic iron-iron site nano-enzyme constructed by relieving oxidative stress and cartilage degeneration and used for targeted osteoarthritis treatment. According to the invention, a nitrogen-doped porous carbon anchored diatomic iron nano enzyme catalyst (Fe2-NCs) with Fe-Fe dimer coordination is developed by using a'subject-object 'strategy. The Fe2-NCs protect cartilage cells from oxidative stress induced apoptosis by modulating ROS and active nitrogen species (RNS) and promoting O2 release. In addition, Fe2-NCs restores mitochondrial function by inhibiting NOX4 expression, improving ATP production, and normalizing COXIV levels. In an in-vivo OA model, the Fe2-NCs can reduce the expression of a pro-inflammatory medium COX-2 through an NF-kappa B signal channel, inhibit the up-regulation of MMP-13 and delay the degradation of type II collagen. The invention provides a new theoretical framework and methodological approach for the treatment of osteoarthritis, and has important clinical and scientific significance.

The invention discloses a method for preparing functional artificial aggregate from lake and reservoir deposits, and belongs to the technical field of preparation of artificial aggregate. The functional artificial aggregate with excellent mechanical properties is prepared by taking the reservoir deposits, the mineral powder and the fly ash as main raw materials, adjusting the ratio of the reservoir deposits to the mineral powder to the fly ash, combining layered sodium disilicate, nano CaO and silicon powder as an alkali activator and an additive, and doping fibers and phase change microcapsules at the same time. The problems that in the prior art, reservoir deposits are difficult to recycle and low in utilization rate, and a traditional alkali excitation system cannot control the early strength are solved.

The present invention discloses a dynamically vulcanized low-temperature-resistant high-impact polypropylene alloy, comprising the following components in parts by weight: polypropylene: 70-90 parts by weight; ethylene-propylene rubber: 5-25 parts by weight; thermoplastic elastomer: 5-15 parts by weight; octylphenol-formaldehyde vulcanized resin: 0.1-3 parts by weight; stannous chloride: 0.008-0.36 parts by weight; zinc oxide: 0.1-0.75 parts by weight; stearic acid: 0.05-0.375 parts by weight; the thermoplastic elastomer is at least one of styrene-hydrogenated isoprene copolymer or styrene-hydrogenated isoprene-styrene copolymer. The present invention uses SEP or SEPS with relatively high low-temperature toughening efficiency to improve the low-temperature toughness of the material, and uses octylphenol-formaldehyde resin as a vulcanizing agent to dynamically vulcanize the ethylene-propylene rubber to improve the strength of the material, and achieves a notched impact strength of the material at -30°C of up to 50kJ / m 2 Under the condition of ensuring that the room temperature tensile strength of the material is not less than 30MPa, the rigidity and toughness balance of the material is achieved.

The preparation method comprises the following steps: crushing raw materials, carrying out dual-frequency ultrasonic extraction in combination with a solvent to obtain a high-activity and high-oxidation-resistance component, filtering through a micro-filtration membrane, mixing an extracting solution and crushed filter residue raw materials according to a ratio, sterilizing, adding modified microcrystalline cellulose and other auxiliary materials, and mixing, so as to obtain the anti-aging body-consolidating and life-prolonging pill. And refining honey and pelleting to prepare the life-prolonging pill with high stability, good pill quality and high oxidation resistance.

The invention discloses uniform mixing equipment for preparing antiviral oral liquid, and belongs to the technical field of pharmaceutical preparation production, the uniform mixing equipment comprises a mixing part, the mixing part comprises a mixing equipment main body, a stirring assembly for mixing liquid medicine is arranged in the mixing equipment main body, and a first sliding groove and a plurality of teeth are arranged in the mixing equipment main body; a buffer thin-wall layer for slowly conducting heat is arranged on the stirring assembly; the cooling part comprises a liquid conveying assembly and a cooling assembly which are arranged in the stirring assembly and is used for uniformly conveying a solvent and cooling a medicament in the mixing process, and through the collaborative design of rotation of a main stirring paddle and synchronous liquid spraying of a liquid outlet, purified water is uniformly dispersed to all positions of an inner cavity of the mixing tank body in the stirring process; the problems that local liquid medicine is too thick and is slowly dispersed due to traditional top single-point water adding are solved, the raw materials and the solvent can be rapidly and evenly mixed without long-time stirring, the mixing period is greatly shortened, and meanwhile heat accumulation generated by long-time stirring is reduced.

The invention belongs to the technical field of medicines, and particularly relates to a solid composition containing ceftizoxime sodium microcapsules as well as a preparation method and application of the solid composition. The invention provides a solid composition containing ceftizoxime sodium microcapsules. The solid composition comprises the ceftizoxime sodium microcapsules, sorbitol, microcrystalline cellulose and povidone, the ceftizoxime sodium micro-capsule comprises a capsule core and a capsule wall material wrapping the capsule core, and the capsule core comprises ceftizoxime sodium. The solid composition containing the ceftizoxime sodium microcapsules provided by the invention is high in ceftizoxime sodium active ingredient content, long in storage period, accurate in dosage and convenient to orally take and use, and can be stably released in an acidic or alkaline environment.

The present application provides a kind of cellulose fiber and its preparation method, first NMMO aqueous solution is heated and stirred, and cellulose raw material is added to the prepared NMMO aqueous solvent, and heated and stirred;Then the blend after swelling is dissolved by stirring and vacuumizing, and then the obtained cellulose solution is continuously heated and stirred, while adding spinning aid, after stirring, centrifugal spinning dope is obtained, finally the obtained spinning dope is injected into syringe, and cellulose filament is spun at low temperature by push pump.The method can reduce the temperature of cellulose spinning relative to the spinning method of NMMO water system, and can effectively reduce the energy consumption of industrial production of cellulose fiber, and the method is obvious at low cellulose concentration, which has great economic advantage in the differentiation production of lyocell fiber.

The invention discloses composite exocarpium citri grandis stuffing with homology of medicine and food and a preparation method of the composite exocarpium citri grandis stuffing, and relates to the technical field of food processing.The preparation method of the composite exocarpium citri grandis stuffing comprises the steps that chopped exocarpium citri grandis and pericarpium citri reticulatae are mixed with rose wine and honey, pickling treatment is conducted under the sealed condition, and a pretreated flavor material is prepared; preparing red beans and white kidney beans into composite bean paste, performing stir-frying dehydration treatment, turning off a heating source after stir-frying is completed, after the temperature of a pot body is naturally reduced, adding the pretreated flavor materials into the pot, performing uniform stirring by utilizing residual heat of the pot body, and finally performing low-temperature refrigeration and ripening to obtain a finished product. The method effectively solves the technical problem that in the prior art, debitterizing treatment of exocarpium citri grandis and retention of aroma and active ingredients are difficult to consider at the same time, bitter substances of the exocarpium citri grandis can be effectively converted, meanwhile, the characteristic aroma and functional active ingredients of the exocarpium citri grandis can be completely retained, and the prepared stuffing is harmonious in flavor, fine and smooth in taste, prominent in functionality and good in taste. The encrusting machine is suitable for encrusting processing of various baked and steamed foods.

The invention belongs to the technical field of in-vitro detection, and particularly relates to a detection reagent, a kit and a detection method for detecting AD target Micro RNA (Ribonucleic Acid) by a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology. According to the present invention, the AD disease detection target Micro RNA and the primer and other nucleic acid sequences are screened, the concentration of each component in the detection system is preferably selected, and the detection reagent and the kit for detecting the target Micro RNA through the CRISPR-circit technology are provided; according to the present invention, the CRISPR-circit detection system is delivered by using the liposome, and the CRISPR-circit technology and the membrane fusion technology are combined so as to achieve the in-situ detection of the exosome MicroRNA, such that the method has advantages of high sensitivity and high specificity, and can accurately and rapidly detect the target MicroRNA in the AD patient serum in situ. The invention provides a rapid and efficient method for detecting the Alzheimer's disease, and the application prospect is good.

The application belongs to the technical field of beverage processing, and discloses a method for stabilizing anthocyanin in high-acid fruit juice in the presence of Vc. A stabilizer is added to fruit juice containing Vc and anthocyanin at a final concentration of 0.1-1.0 g / L, then inert gas is introduced into the fruit juice and ultrasonic degassing is performed, and the dissolved oxygen content in the fruit juice is controlled to be lower than 1 mg / L. The stabilizer is a mixture of sinapinic acid, caffeic acid and tea polyphenol, and the mass ratio of sinapinic acid, caffeic acid and tea polyphenol is 5-25:5-25:0.03-3. In one aspect, the application uses traditional sinapinic acid and caffeic acid as raw materials to inhibit the mutual promotion degradation between Vc and anthocyanin. In another aspect, the application innovatively uses tea polyphenol as a stabilizer.

This invention discloses a method for extracting high-purity PDRN from salmon testes and its application in improving skin condition, belonging to the field of bioactive ingredient extraction technology. Frozen salmon testis tissue is cleaned of blood vessels and fatty impurities, then cryogenically pulverized at -20~-25℃. The pulverized tissue is added to a pre-treatment dispersion buffer and homogenized under high pressure to obtain a DNA fragmentation solution. The precipitate is then separated by centrifugation. The precipitate is dispersed in a dedicated enzyme digestion buffer, and enzymatic digestion with protease and RNase A is performed to inactivate the enzyme. DNase I is added to the digestion solution for enzymatic cleavage. After the reaction, a stop solution is added to terminate the reaction, and the supernatant is separated by centrifugation. Ethanol is added to the supernatant for precipitation, and centrifugation yields PDRN from salmon testes. This invention, through specific buffer systems and enzymatic digestion conditions, increases the PDRN content by 40~60 kDa, allowing for better absorption and utilization by the skin, improving facial hydration and elasticity, effectively reducing facial wrinkles, and improving facial skin condition. It can be used to prepare functional cosmetics.

This invention relates to the field of flue gas treatment technology, specifically to a sulfur dioxide gas absorbent, comprising the following raw materials by weight: 90-100 parts of an aqueous amine solution and 2-6 parts of additives. In this invention, the nitrogen-zinc ion-sulfur active center of the catalyst acts as a molecular key, efficiently and reversibly coordinating and activating sulfur dioxide molecules, significantly reducing the reaction energy barrier and fundamentally improving the intrinsic absorption rate. The enhancer, with its high specific surface area, provides a large interface for the reaction. Its surface sulfonic acid groups not only buffer the decrease in system pH, maintaining the optimal acid-base environment for the reaction, but also catalyze the conversion of absorption intermediates into more stable products, thereby shifting the reaction equilibrium to the right. Under the combined effect of these two factors, the catalyst accelerates the reaction towards equilibrium, while the enhancer continuously shifts the equilibrium point, resulting in a higher absorption capacity for the absorbent.

The present invention relates to a composition for preventing, improving, or treating degenerative arthritis comprising a steamed ginger extract or 1-dehydro-6-gingerdione isolated therefrom as an active ingredient. The steamed ginger extract according to the present invention or 1-dehydro-6-gingerdione isolated therefrom exhibits an effect of inhibiting inflammatory cytokine expression, cartilage tissue recovery, cartilage production, or cartilage degradation, and inhibits the expression of I-xB or NF-κB protein involved in inflammatory cytokine and MMP production, and thus is expected to be effectively used in functional food compositions, pharmaceutical compositions, or the like for preventing, improving, or treating degenerative arthritis.

The invention relates to the field of food, and particularly discloses a kudzuvine root, rape pollen and bean composition and a preparation method thereof, and the kudzuvine root, rape pollen and bean composition comprises the following raw materials in parts by weight: 40-60 parts of rape pollen, 20-40 parts of kudzuvine root powder, 1-10 parts of rhizoma polygonati powder, 1-10 parts of smoked plum powder, 1-10 parts of Chinese yam powder and 1-10 parts of maltodextrin. The preparation method comprises the following steps: S1, preparing materials, namely crushing and sieving the raw materials, and weighing the prepared materials; and S2, mixing the raw materials, granulating, drying, tabletting, and packaging to obtain the kudzuvine root, rape and pollen bean composition. The pollen nutritional supplement has the characteristic that the functionality and the absorptivity of the pollen nutritional supplement can be enhanced.

This utility model provides an intelligent temperature-controlled mixing device for plastic masterbatch, relating to the field of plastic product manufacturing technology. It includes: a fixing ring, a support rod, a temperature control component, a plastic substrate granule feeding hopper, a masterbatch feeding hopper, a mixing mechanism, a discharge pipe, and a switching valve. The temperature control component includes an outer shell, an inner shell, a heating element, and a control panel. The plastic substrate granule feeding hopper includes a feeding pipe, a feeding hopper, and a filter screen. The mixing mechanism includes a servo motor, a first rotating rod, a spiral stirring blade, a collar, a flat stirring blade, and a connecting component. The connecting component includes a second rotating rod, a lower stirring blade, and a connecting rod. This utility model can significantly improve the efficiency and quality of plastic masterbatch mixing and reduce the intensity of manual operation.

The invention belongs to the technical field of biological fermentation, and provides a microbial fermentation efficient extraction process and device, and the process comprises the following steps: step 1) continuous disinfection; the method comprises the steps of (1) fermentation, (2) fermentation culture, (3) cross-tank conveying, (4) temperature control and high-pressure recovery, (5) split-flow extraction and purification and (6) refining and drying, the quality of fermentation liquor is improved through a fermentation process precise control technology and a new fermentation technological process, and the product yield is improved through an efficient extraction technology.

The invention discloses a preparation method of dimeticone aerosol, and belongs to the technical field of pharmaceutical preparations. The method comprises the following steps: (a) adding 0.6%-1% by mass of dimeticone and 0.25%-0.58% by mass of menthol into a medicinal aluminum tank or stainless steel tank with the inner wall coated with an inert material, and stirring for 5-30 minutes at 100-500 rpm under the protection of nitrogen to form uniform and transparent liquid; (b) sealing and filling dimethyl ether with the purity not lower than 99.9% at the temperature of 10-20 DEG C to serve as a propellant, and enabling the content of the dimethyl ether to reach 98.42%-99.15%; and (c) valve assembly and sealing are completed under the conditions that the temperature is 15-30 DEG C and the relative humidity is smaller than or equal to 60%, and standing balancing is conducted for 12-48 hours at the temperature of 25 DEG C. According to the dimeticone propellant disclosed by the invention, the problems of poor compatibility of dimeticone and the propellant, instability in long-term storage, easiness in layering or valve blockage and the like are effectively solved by virtue of synergistic measures of limiting the component proportion, adopting high-purity dimethyl ether, controlling the particle size of menthol, using an inert coating container, protecting by nitrogen and the like.

The utility model relates to the technical field of earthworm protein processing, in particular to a multifunctional liquid nitrogen device for earthworm protein production, which comprises a processing cabinet, a cabinet door, a blanking hopper, a hopper plug, a liquid inlet, a connecting pipe, a liquid nitrogen spray head and the like, a cabinet door is rotationally connected to one side of the treatment cabinet, a discharging hopper is fixedly connected to the top of the treatment cabinet, a hopper plug used for sealing an opening of the discharging hopper is movably connected to the discharging hopper, a hopper neck of the discharging hopper extends into the treatment cabinet and is provided with a liquid nitrogen spray head, a liquid inlet is formed in the position, close to the discharging hopper, of the treatment cabinet, and the liquid inlet is communicated with the liquid nitrogen spray head through a connecting pipe. According to the quick-freezing and grinding device, through cooperation of the liquid nitrogen spray head, the grinding frame, the grinding rod, the driving mechanism and other parts, tight connection of quick-freezing work and grinding work is achieved, then the wind direction of degradation and denaturation caused by raw material transfer is avoided, and the production efficiency and the product quality are effectively improved.

This invention relates to the field of biodiversity monitoring technology and discloses a monitoring and alarm device for rare and endangered aquatic organisms based on environmental DNA. The device includes a sampling and enrichment unit, a nucleic acid in-situ extraction and purification unit, a isothermal fluorescence detection unit, an environmental factor monitoring unit, a main control and data transmission unit, a power supply and protection unit, and a remote alarm and control platform. The main control and data transmission unit obtains the pressure change rate based on the digital pressure signal output by the microcomputer-controlled pressure sensor, extracts the high-precision peristaltic pump operating time parameter to calculate the actual filtration volume, and performs a division operation to obtain the environmental DNA calibration concentration. It then calculates the dynamic transmission power value by combining the network signal strength indicator value and transmits the complete communication data packet. The remote alarm and control platform obtains a risk index based on the environmental DNA calibration concentration, maps the spatial coordinate signal to an electronic map module to generate coordinate nodes, and displays the risk index and digital environmental parameters in association.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belong to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

The invention belongs to the technical field of fuel additives, and particularly relates to a preparation method of micro-quantum core mother liquor for an internal combustion engine. S1, raw material pretreatment and solvent dehydration; s2, preparation of a combustion and calorific value enhancement functional mother solution A; s3, preparation of a system protection function mother solution B; s4, preparing a mother solution C with stable operation and lubricating functions; and S5, compounding, degassing and finely filtering the three functional mother liquor. An eight-element synergistic system is constructed, and the micro-quantum core mother liquor for the internal combustion engine is prepared by adopting a three-module step-by-step preparation process; after methanol fuel is added, the combustion efficiency, power output and cold start performance can be synergistically improved, meanwhile, electrochemical corrosion, sealing element swelling and part abrasion are inhibited, and it is guaranteed that the fuel is stably stored for a long time; the process avoids the compatibility problem of multi-additive mixing, and solves multiple contradictions of energy improvement, material protection and fuel stability.

The invention relates to the technical field of traditional Chinese medicine and health food processing, and discloses a preparation process and application of a black ginseng extract lozenge, and the preparation process comprises the following steps: crushing starch, powdered sugar and polyethylene glycol in parts by weight, and sieving for later use; performing ultrasonic extraction and vacuum concentration on the black ginseng to obtain a black ginseng concentrated solution; slowly adding a pre-prepared black ginseng concentrated solution into the sieved starch and powdered sugar, and stirring to prepare a soft material; granulating, drying and finishing the soft material to obtain dry granules; and adding sieved polyethylene glycol into the dry particles, uniformly mixing, tabletting, forming, and sterilizing by ultraviolet radiation to obtain the buccal tablets. According to the method, ultrasonic-assisted extraction is adopted, so that degradation of heat-sensitive rare saponin is avoided; the concentrated solution is directly granulated, so that the difficulty that the extract absorbs moisture and is sticky is overcome, and the forming rate is increased; the prepared lozenge can activate a PI3K-Akt signal channel and regulate release of 5-HT, and can be used for preparing products for relieving chronic fatigue syndrome and improving spatial cognitive impairment.

The invention discloses a biochar-based coated selenium slow-release fertilizer and a preparation method thereof, and belongs to the technical field of fertilizers. The fertilizer comprises a core layer and a coating layer coated on the surface of the core layer, and the raw materials of the core layer comprise the following components by mass: 70-80 parts of urea, 60-70 parts of monoammonium phosphate, 50-60 parts of potassium chloride, 30-40 parts of superphosphate, 2-3 parts of sodium silicate, 2-4 parts of magnesium sulfate, 8-15 parts of nano-selenium, and 4-6 parts of tourmaline powder; the raw materials of the coating layer comprise the following components: 120-150 parts of modified biochar powder, and 20-30 parts of modified starch / polyvinyl alcohol composite. The fertilizer has good slow-release effect, high utilization rate of selenium element, good fertilizer efficiency, can effectively improve the yield and quality of crops, and has a simple preparation process, low cost, and no pollution.

The application discloses an eye lotion containing PHMB and a preparation method thereof, and belongs to the field of eye care preparations. The eye lotion takes PHMB as a bacteriostatic component, is matched with isotonicity regulators, buffers, humectants and EDTA2Na, and simultaneously adds trehalose and allantoin to construct a synergistic stable protection system. The eye lotion is prepared through a process flow of low-temperature light-shielding, preparation of a base liquid first and then addition of PHMB, two-stage sterilization filtration and aseptic filling. The application solves the technical problems that PHMB is easy to degrade in a liquid preparation, has poor stability, and the effective component is easy to attenuate after opening and use. Meanwhile, EDTA2Na and PHMB have a synergistic effect, trehalose and allantoin further stabilize the PHMB structure from a molecular level and inhibit oxidative degradation, so that the product can realize wide-spectrum and high-efficiency bacteriostasis at a low concentration. The obtained eye lotion is mild and non-irritating, clear and stable, and can still maintain stable physicochemical and bacteriostatic properties for a long time after opening, is suitable for industrial production, and can be used for daily eye cleaning, flushing, moisturizing and bacteriostatic care.

The invention discloses a stem cell-based microvesicle as well as a preparation method and application thereof, belongs to the technical field of biological tissue engineering, and particularly relates to a preparation method of the stem cell-based microvesicle, which comprises the following steps: culturing stem cells until the cell fusion degree is 80-90%, performing hypoxia treatment, and cleaning for 2-3 times; standing and culturing the cleaned stem cells, then adding a calcium ion carrier, and carrying out ultrasonic treatment; the serum-free basic culture medium containing the calcium ion carrier is immediately absorbed and removed, the cells are cleaned through PBS, culture is conducted for 24 h, and cell supernatant is collected and merged respectively; performing first centrifugation to remove cell debris; performing second centrifugation to remove dead cells and large cell debris; carrying out third centrifugation to remove organelles; fourth centrifugation is carried out, large vesicles are removed, and a final supernatant is obtained; and filtering and centrifuging to obtain a microvesicle precipitate. The preparation method of the microvesicles aims at systematically improving the yield and biological activity of the stem cell microvesicles through low-oxygen pre-stimulation, ultrasonic-calcium ion carrier synergistic induction and differential centrifugation.

This invention provides an inorganic-organic composite solid electrolyte membrane and its preparation method, belonging to the field of solid electrolyte membrane technology. The invention uses anisole and tetrahydrofuran as a mixed solvent to dissolve methyl-terminated PEO. Tetrahydrofuran weakens the interaction between the ether oxygen atoms on the PEO chain and anisole, promoting the dispersion of PEO in the mixed solvent and facilitating the formation of a homogeneous coating slurry. Meanwhile, methyl-terminated polyethylene oxide not only improves the loss of ionic conductivity after the sulfide solid electrolyte is composited with a non-ionic binder, but its methyl termination also inhibits the degradation effect of polyethylene oxide on the sulfide solid electrolyte, better preserving the high-performance characteristics of the sulfide. Furthermore, the addition of Li3PO4 increases the lithium ion transport rate in polyethylene oxide, thereby improving the ionic conductivity of the composite solid electrolyte membrane.