62 results about "Nostoc" patented technology

The invention discloses a cultural method and device for common nostoc. The method comprises the following steps of: a, preparation of nostoc seed: collecting natural, wild and common nostoc; b, inoculation: uniformly spreading water white water retaining agent after being saturated by absorbing water onto a culture plate and spreading the nostoc seeds onto the water retaining agent after being saturated by absorbing water; c, preparation of a nutrient medium, wherein the nutrient medium is composed of three parts; d, control of a cultural process: controlling the periodic cycle of the nutrient solution in a natural light period and generating power by adopting a photovoltaic panel to drive a water pump, thus a nutrient salt solution periodically returns and flows; and e, harvesting: turning off a water pump, carrying out natural air drying, dehydrating and contracting thallus and harvesting the thallus. The culture plate is fixed by a fixed support, a water tank is respectively connected with a water pump and a shading shed by virtue of guide pipes, the water pump is connected with a water tank by virtue of a guide pipe, the water tank is respectively connected with the water pump and a water collecting tank by virtue of guide pipes, and the water pump is connected with the photovoltaic panel. The method disclosed by the invention can be used for solving the problems that in the traditional cultural method, nostoc grows slowly, the yield is low, the thallus is loose and the quality is poor.

The invention discloses a domestication method of nostoc and application thereof. The domestication method comprises the following steps of: preparing a specific culture medium; carrying out salt tolerance domestication, nitrogen and phosphorus content reduction domestication, domestication of adaptability to water to be treated, and the like on screened nostoc species; carrying out step-by-step amplification culture; then leading the nostoc species into a saline and alkaline land, wherein the nostoc is propagated in large quantities by utilizing photosynthesis and absorbs and fixes nitrogen and phosphorus elements contained in atmosphere and water sources; and after the nostoc is propagated, draining culture water for the nostoc, wherein the nostoc is deposited in soil and contains a large quantity of elements, i.e. nitrogen, phosphorus, and the like. After primary treatment is carried out by using the domestication method, the nitrogen contained in the soil is increased by 18-21 percent, the phosphorus is increased by 12-14 percent, and full salt content is reduced by 15-18 percent, therefore not only is nutrient element loss caused by a water washing method prevented, but also the hardening of the soil and the secondary pollution to the water sources and environments, which are caused by chemical fertilizer application, are prevented; and in addition, soil fertility is gradually enhanced and soil restoration is accelerated through multi-time treatment, therefore the purpose of improving an ecological environment is finally achieved.

The invention relates to a method and a system for the mass production and the coupling carbon fixation and emission reduction of healthy green food common nostoc, which realize large-scale high yield and carbon fixation and emission reduction by using a semiclosed indoor pool on the basis of realizing the high yield of the common nostoc and disclose a structural principle of the pool and optimization conditions of seven basic elements for culturing the common nostoc. The method is suitable for the urban and rural large-scale production, is especially suitable for poor grain lacking regions and is also suitable for the food self-supporting production of global extreme environments and disasters and spaceman living environments. The invention provides a novel healthy green food for human and also realizes the emission reduction, the oxygen generation, and the environment improvement in the food production process.

The invention discloses a making method of a nostoc sphaeroids kutz beverage. The making method of the nostoc sphaeroids kutz beverage comprises the following steps: uniformly mixing 15-25 parts of fruit powder, 10-15 parts of nostoc sphaeroids kutz plant water, 60-70 parts of purified water, 5-10 parts of white granulated sugar and 6-12 parts of citric acid in proportion, then sterilizing the mixed liquid for 3 minutes at the temperature of 90 DEG C, then cooling to the temperature of 40 DEG C, selectively adding 5-10 parts of nostoc sphaeroids kutz polysaccharide dry powder, finally adding aplurality of round and elastic fresh nostoc sphaeroids kutz in mixed liquid, and filling a beverage bottle with the mixed liquid in a sterile environment to obtain the nostoc sphaeroids kutz beveragewith nostoc sphaeroids kutz granules. The nostoc sphaeroids kutz plant water is added in the prepared nostoc sphaeroids kutz beverage, the nutrient utilization rate is increased, and furthermore, theproduction cost can be reduced; and the nostoc sphaeroids kutz polysaccharide dry powder is added, the use amount of a preservative is reduced, and furthermore, a nutrition and health care effect isachieved; and by addition of the fresh nostoc sphaeroids kutz, the visual effect of the beverage is improved, furthermore, the nutritional effect of the beverage is enhanced, and the elastic granularfeeling of the beverage is improved.

The invention discloses a selenium-enriched common nostoc culture technique. The selenium-enriched common nostoc is produced through strain domestication, selenium-enriched common nostoc culture, harvesting and post-processing, can be used for health-care foods, beverages and medicinal raw materials and has the treating effects on clearing away internal heat, improving eyesight, resisting aging and infection and the like and has certain preventing and auxiliary treating effects on cancers, heart disease, Keshan disease, cataract, atherosclerosis and the like.

The invention relates to broad-spectrum virulent cyanophage Me-ZS1 and application thereof, and relates to biological treatment of water blooms, wherein the Me-ZS1 is preserved in China General Microbiological Culture Collection Center, and the serial number of the Me-ZS1 is CGMCC No. 16812. According to the invention, Me-ZS1 is cyanophage obtained by separating the first strain by taking the toxin-producing microcystis elabena as a target, and has cracking effects on Microcystis aeruginosa FACHB-905, FACHB-925, FACHB-469 and FACHB-942 strains under Chroococcales, M .wesenbergii FACHB-908, FACHB-1112 and FACHB-929 strains, M .flos-aquae FACHB-1028 strain, M .elabena FACHB-916 strain, Microcystis sp .7806 FACHB-915 strain, Nostoc sp. FACHB-596 strain under Nostocales, and Anabaena flosaguasFACHB-245 strain.

The invention relates to a nostoc sphaeroids kutz algae colonization induction method. The nostoc sphaeroids kutz algae colonization induction method comprises the following steps: a nostoc sphaeroidskutz group is induced into a grey green group with an algae colonization release signal; a mild tearing method is utilized, so that the group completely loses osmotic protection, and under the assistance of ventilation and multiple induction of violent condition changes before and after ventilation, algae filaments are broken and differentiated into nostoc sphaeroides colonization sections. The method has the beneficial effects that under carbon source deficiency under absolute vacuum and under the synergistic effect of temperature and illumination conditions for enhancing photosynthesis of the nostoc sphaeroides are supplemented, nostoc sphaeroides group pigments are induced to be degraded into nostocsphaeroides groups with algal colonization segment release signals, ventilation induction after tearing is adopted, the algae filaments can smoothly break colloid sheaths to come out to be broken off to obtain algal colonization segments with gas vesicles to be uniformly dispersed in aninduction culture solution, the algal colonization segment is uniformly dispersed in the induction culture solution, such that the algal colonization segment induction period is substantially shortened, the mechanical damage is extremely low, the nutrition and the illumination required by the algal colonization segment development are provided during the induction process, and the algal colonization segment development activity is effectively enhanced.

The invention relates to methods and devices for adsorption of air pollutants by nostoc. The method comprises following steps: performing concentrated extraction of contaminated air from an environment with air pollutants by an air intake; and introducing the extracted air into a pre-set aeration disk arranged inside the candidal culture area; air pollutants contained in the gas entering the aeration tank are adsorbed by candida within the candidal culture zone; the gas completes circulation in the candidal culture zone and is drawn along the outlet. The invention adsorbs air pollutants existing in the air by the candida in the cultivation area , so that the air discharged from the cultivation area of candida can reduce the content of air pollutants , and at the same time , allows the candida to continue to grow and adsorb more air pollutants during the feeding process , so that the monilium grown algae can be re-applied , thus effectively blocking the secondary air pollutants existing in the air pollutants .

The invention provides a method for cultivating common nostoc by using a farmland. The method comprises the following steps: selecting and arranging the farmland, preparing algae, and doing preparatory work; watering and leveling in a cultivated field to seed after a week; adjusting the pH value of water in the cultivated filed before seeding; spreading the algae into the cultivated field when seeding, and exposing on the surface soil in the cultivated field; keeping the depth of the water in the cultivated field at 2-16cm, so that a common nostoc group is completely submerged below the water level; keeping the soil layer and the water layer stable in the cultivated field; adding magnesium chloride, calcium chloride and dipotassium phosphate to the cultivated field every 15-30 days; harvesting the common nostoc group after cultivation is ended. The method for cultivating the common nostoc by using the farmland provided by the invention is simple and feasible to operate, and low in cost, the free farmland is fully utilized in winter, the produced common nostoc product is good in quality, the method can be widely applicable to the requirements of large-scale production of the common nostoc, and the income of local farmers is also increased.

The invention discloses a nostoc high-efficiency cracking phycophage as well as a separation method and application thereof. The nostoc high-efficiency cracking phycophage is characterized in that the phycophage is a novel Myoviridae phycophage, is named as YongM, is preserved in the General Microbiological Center of Microbiological Culture Collection Center on January 13, 2020, and is numbered as CGMCC NO.18383. The YongM has the characteristics of high efficiency and broad spectrum, can be used for efficiently splitting nostoc and can be used for infecting and splitting various microcystis, Chroococcus, Anabaena aquatica, oscillatoria planktonic and floating hylonema adami. According to the nostoc high-efficiency cracking phycophage, with the application of the YongM, the algae liquid of the nostoc FACHB-596, the algae liquid of the anabaena flos-aquae FACHB-245, the algae liquid of the Chroococcus FACHB-193, the oscillatoria planktonic FACHB-708 can be subjected to cracking clarification within 24 h.

The invention discloses a constructing and expressing cleanse method of pET-SOD engineering bacteria, which comprises the following steps: designing primer to acquire Fe-SOD gene from pET-SOD plasmid of nostoc CHEN Fe-SOD; designing primer to acquire ScFv gene from T-ScFv plasmid of ScFv; restructuring emzyme-cutting Fe-sod gene and ScFv gene; acquiring SOD-ScFv fusion gene; restructuring in pET28a(+) plasmid carrier; constructing to acquire Pet-SOD-ScFv fusion carrier; conversing to recipe bacillus coli BL21; obtaining Pet-SOD-ScFv engineering bacteria; filling IPTG and Fe3+ to evoke; gaining SOD-ScFv expression.

The invention discloses a composition for prenatal care and aims to implement prenatal care intervention. The composition is characterized by comprising the following active pharmaceutical ingredients in parts by weight: Bulbophyllum inconspicuum Maxim, unpleasant swallowwort herb, Magnolia amoena, Sargassumthunbergii, lychee seed, yellow gelatin, Tetrapanax papyriferum, poncirus bark, common nostoc, dendrobe, sun-lo and fistular onion stalk. The clinical experiment proves that the composition for prenatal care is suitable for prenatal application, has the characteristics of favorable curative effect and high safety, and is worthy of clinical application and popularization.

The invention provides a culture method and culture apparatus for common nostoc, belonging to the field of microalga culture technologies. The culture method comprises a step of subjecting common nostoc to static culture in a thin-layer culture solution so as to allow the common nostoc to directly utilize CO2 adsorbed on the surface of the thin-layer culture solution. The culture apparatus is a laminated microalga thin-layer culture apparatus. The culture method and culture apparatus provided by the invention can increase biomass output per unit area of a light source. Energy consumed in carbon supply, stirring and the like can be omitted through thin-layer and static culture, so unit cost is effectively lowered.