18 results about "Aquilaria" patented technology

The invention discloses an identification method of agilawood germplasm resources of aquilaria agallocha. The identification method is characterized in that seven pairs of primer sites corresponding to DNA molecular markers of seven genome SNP sites are provided; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification and primer effect test, carrying out enzyme digestion on a PCR product, and distinguishing agilawood and aquilaria sinensis according to an enzyme digestion result, and / or distinguishing different varieties of agilawood; if the seven pairs of primer loci are not subjected to enzyme digestion, judging the aquilaria sinensis as aquilaria sinensis. Effective amplification of the agilawood molecular marker is realized by adopting a conventional PCR technology, and the method has universality; genotype identification is completed by integrating an enzyme digestion method with agarose gel, an expensive sequencer is not needed, and the method has the technical advantages of being easy and convenient to operate, low in cost and the like; the polymorphism of genotypes, including homozygous genotypes and heterozygous genotypes, can be accurately identified.

The application discloses application of an alcohol extract of white wood of Aquilaria sinensis in preparation of anti-inflammatory products. The white wood of Aquilaria sinensis extract is obtained by using white wood of Aquilaria sinensis as raw material, first extracting and degreasing with petroleum ether, then extracting with ethyl acetate, and then extracting with ethanol or ethanol aqueous solution. The application provides a new high-value utilization way for the white wood of Aquilaria sinensis which is a rich but underutilized natural resource. The extract Fr3 as a natural source of anti-inflammatory active substance has a wide application prospect in preparation of anti-inflammatory drugs, health products and functional foods and other products.

The invention belongs to the technical field of biological medicine, and particularly relates to a rapid identification method of aquilaria agallocha and common aquilaria agallocha and application of a novel marker. The invention firstly provides a rapid identification method, and the rapid identification method is a thermal cracking-gas chromatography-mass spectrometry combined method. The method comprises the following steps: taking a to-be-detected agilawood sample, carrying out thermal cracking at 550-650 DEG C, and analyzing and identifying a characteristic peak of a mass spectrum of a thermal cracking product. The method is short in time consumption, the whole identification process only needs dozens of minutes, and the method is low in cost. In addition, the analysis of the identification result only needs to judge the size of the characteristic peak of the mass spectrum, and the result is convenient to read and clear at a glance.

The application discloses a plough head mold GXU-F34 for promoting linaloe, and the plough head mold GXU-F34 is obtained by purifying and separating samples cut from old trees and can be used for promoting linaloe. The plough head mold GXU-F34 is a plant endophyte, can continuously produce some enzymes to stimulate the defense system of Aquilaria plants, and a series of enzymes produced by saprophytic fungi microorganisms play an important role in the defense mechanism of Aquilaria plants, and finally lead to the production of linaloe.

The invention belongs to the technical field of air purification, and particularly relates to a composition containing agilawood essential oil and application of the composition in air purification products. The aquilaria agallocha essential oil, the catechu extract, the hibiscus mutabilis extract and the chitosan are compounded into the air purification component, so that the composition containing the air purification component has remarkable and lasting formaldehyde and ammonia removal effects, and the problem that an existing air purification product is poor in odor removal effect and not lasting in odor removal effect can be solved. According to the preparation method of the agilawood essential oil, three extraction agents including absolute ethyl alcohol, acetone and diethyl ether are sequentially adopted for carrying out low-temperature continuous phase change extraction on agilawood powder, and the method has the advantages of being high in safety, high in extraction rate, easy to operate and capable of achieving all-around efficient extraction. The composition provided by the invention also has the advantages of simple and convenient preparation method, suitability for industrial production and the like, can be applied to preparation of air purification products, and has better application value.

A culture box comprises an outer box body and partition plates, the outer box body is detachably connected to a mounting plate, the partition plates are arranged in the outer box body and divide the outer box body into an air inlet channel and a plurality of culture spaces, and the culture spaces are arranged at intervals in the circumferential direction of the air inlet channel; the partition plate is provided with a plurality of air inlet holes penetrating through the two opposite plate faces of the partition plate, and the outer box body is provided with air inlet holes communicating with the air inlet channel. The placing rack can be accommodated in a culture space and is used for placing a culture dish; an arc-shaped control plate of the air inlet control structure is arranged in an air inlet channel in a sleeved mode and attached to the side wall face of the air inlet channel, an air passing through hole is formed in the arc-shaped control plate, and a control driving piece is arranged in the air inlet channel and is in transmission connection with the arc-shaped control plate. The agilawood tissue culture seedlings in different growth stages can be stored at the same time, and the humidity in different environment spaces can be conveniently controlled according to needs.

The invention discloses an agilawood drilling and producing method. The method comprises the following operation steps: S1, selecting an agilawood tree: selecting an agilawood tree which is straight, free of damage and pest and disease damage, more than or equal to five years old and more than 5cm in diameter at breast height; s2, drilling: drilling a row of holes which are vertically arranged at intervals of 4-16 cm in the trunk from bottom to top to the crown; s3, after drilling is completed, all branches below the tree crown are cut off, and the tree is obtained. The method follows the natural growth rule of the agilawood tree, does not cause large-scale damage to the tree body, has the advantages of rapidness and simplicity, and is low in labor demand and low in cost; when the method is adopted to produce agilawood, the content of agilawood essential oil can reach 40.74%, the agilawood quality is good, the agilawood production quality is close to natural quality, and market requirements are met; according to the method, the agilawood producing time is shortened, agilawood can be produced after treatment is conducted for 6 months, the agilawood producing area is large along with time prolonging, the agilawood quality is better, and the relative content of chromone and sesquiterpene in agilawood oil is greatly increased.

This invention discloses an effective method for protecting the phloem tissue of agarwood trees from decay and resin formation, comprising the following steps: Selection of agarwood trees: Select agarwood trees over 3 years old with a diameter at breast height (DBH) of over 10 cm; Drill blind holes towards the tree's axis using a non-threaded drill bit, with a hole diameter of 5-8 mm and a depth of 4-5 cm; Arrange the holes in a stepped pattern from bottom to top, with a horizontal distance of 3-4 cm and a vertical distance of 1-2 cm between each hole and the next hole in the same row, and a vertical distance of 5-6 cm between adjacent rows; Introduce the resin-forming agent into the holes, and then apply a protective solution to the hole openings. This method effectively protects the phloem tissue of agarwood trees, achieving resin formation without decay. After resin formation, the heartwood accumulates yellowish-brown or reddish-brown resin, exhibiting a distinct agarwood layer structure, and the agarwood oil contains higher levels of effective components such as agaritol and agarwood aldehyde.

This invention discloses a method for extracting total triterpenes from the leaves of *Aquilaria sinensis*, comprising the following steps: S1 Raw material pretreatment: *Aquilaria sinensis* leaves are naturally air-dried, then pulverized and sieved; S2 Enzymatic hydrolysis: A compound enzyme is added for enzymatic hydrolysis; S3 Ultrasonic extraction: Ethanol solution is added to the filter residue after enzymatic hydrolysis for ultrasonic-assisted extraction; S4 Purification: The extract is purified by adsorption and elution using macroporous resin to obtain the final product. This method combines enzymatic hydrolysis and ultrasonic-assisted extraction technologies, minimizing damage to the raw materials and offering advantages such as simple operation, high extraction efficiency, high yield, low labor requirements, and low cost. The total triterpenes produced using this method have high purity, with an extraction yield of up to 5.98%, and exhibit significant in vitro antioxidant activity, effectively scavenging DPPH and ABTS free radicals, meeting the demands of the pharmaceutical and health product markets. This method significantly improves extraction efficiency; under optimized conditions, the total triterpenoid content is significantly increased, with purity rising from 15 µg / mL to 33 µg / mL.

The utility model provides an agilawood tissue culture temperature control device, and belongs to the technical field of biological culture. The agilawood tissue culture temperature control device comprises a temperature control assembly and a bearing assembly, the temperature control assembly comprises a box body, a warm air blower, a connecting pipe, a short ventilation pipe, a long ventilation pipe, a temperature control panel and a temperature sensor, the bearing assembly comprises a placing plate frame, a limiting frame and a positioning knob, the placing plate frame is arranged in the box body, and the limiting frame is arranged in the box body. By arranging the box body, the warm air blower, the connecting pipe, the short ventilation pipes, the long ventilation pipes, the temperature control panel and the temperature sensor, hot air of the warm air blower can be evenly introduced and dissipated into the box body by means of alternate arrangement of the multiple short ventilation pipes and the multiple long ventilation pipes, so that the internal temperature of the box body is evenly increased, and the temperature of the box body is increased; by arranging a placing plate frame, a limiting frame and a positioning knob, a plurality of groups of agilawood tissue culture bottles can be limited and separated, and the same culture environment of each culture bottle is ensured to a great extent.

The invention discloses a method for identifying aquilaria agallocha and aquilaria sinensis by using a KASII gene. The agilawood and the aquilaria sinensis are identified on the basis that the KASII genotype of the agilawood is TT and the KASII genotype of the aquilaria sinensis is AA. 20 # basic groups of KASII gene coding regions of aquilaria sinensis and aquilaria sinensis are taken as target sites, a PCR (Polymerase Chain Reaction) and Sanger sequencing method is adopted, a PCR amplification product of the KASII gene is subjected to Sanger sequencing, if the genotype of the KASII is TT, the product is the aquilaria sinensis, and if the genotype of the KASII is AA, the product is the aquilaria sinensis. According to the method for identifying the agilawood agilawood and the aquilaria sinensis through the KASII gene, high-throughput sequencing is carried out on the agilawood agilawood and the aquilaria sinensis, genotype detection analysis is carried out in a whole genome range, and it is found that the KASII gene has significant difference between the agilawood agilawood and the aquilaria sinensis, and the gene function is clear. The method is used for identifying the germplasm of the agilawood agilawood and the aquilaria sinensis by utilizing the genotype difference of the agilawood agilawood and the aquilaria sinensis and adopting PCR amplification and Sanger sequencing methods

The invention discloses a composition containing agilawood extract and a preparation method and application thereof, and belongs to the technical field of biology, the composition comprises 5-20 parts of agilawood extract, 20-60 parts of functional peptide and 5-25 parts of seaweed functional component; wherein the seaweed functional component is used for forming an embedding, gel or slow-release structure; the functional peptide is preferably chlorella pyrenoidosa peptide and is prepared by combining freeze-thaw ultrasonic-assisted enzymolysis with cation exchange chromatography; the agilawood extract is preferably an agilawood chromone enriched extract; during preparation, the seaweed functional component is dissolved in water, the agilawood extract is added for dispersion or emulsification, then the functional peptide is added for uniform mixing, and freeze-drying, spray-drying or forming treatment is performed to obtain the seaweed functional peptide. The composition can effectively improve the stability and bioavailability of active ingredients, and has a good application prospect in preparation of products for auxiliary protection of acute, alcoholic, drug-induced or fatty liver injuries.

The utility model relates to the technical field of grafting devices, in particular to an aquilaria agallocha grafting device. Comprising a base, a first sliding hole, a second sliding hole, a third sliding hole and a fourth sliding hole which are perpendicular are formed in the base, a first containing table is slidably installed on the first sliding hole, a second containing table is slidably installed on the second sliding hole, a third containing table is slidably installed on the third sliding hole, and a fourth containing table is slidably installed on the fourth sliding hole; when the first placing table, the second placing table, the third placing table and the fourth placing table are located on the same straight line, a first cutting mechanism used for cutting stocks is installed between the first placing table and the second placing table, and a second cutting mechanism used for cutting scions is installed between the third placing table and the fourth placing table. By means of the structure, the stock or the scion does not need to be cut manually during cutting, and therefore the cutting quality of the stock and the scion is improved.

The invention discloses a method for identifying aquilaria agallocha and aquilaria sinensis by using a DLAT gene. Aquilaria agallocha and aquilaria sinensis are identified on the basis that the DLAT genotype of the Aquilaria agallocha is TT and the DLAT genotype of the aquilaria sinensis is CC. A No.1879 basic group of a DLAT gene coding region of agilawood aquilaria sinensis and aquilaria sinensis is taken as a target site, a PCR and Sanger sequencing method is adopted, a PCR amplification product of the DLAT gene is subjected to Sanger sequencing, if the genotype of the DLAT is TT, the product is agilawood aquilaria sinensis, and if the genotype of the DLAT is CC, the product is aquilaria sinensis. According to the method for identifying the agilawood agilawood and the aquilaria sinensis through the DLAT gene, high-throughput sequencing is carried out on the agilawood agilawood and the aquilaria sinensis, genotype detection analysis is carried out in a whole genome range, and it is found that the DLAT gene has significant difference between the agilawood agilawood and the aquilaria sinensis, and the gene function is clear. The method is used for identifying the germplasm of the agilawood agilawood and the aquilaria sinensis by utilizing the genotype difference of the agilawood agilawood and the aquilaria sinensis and adopting PCR amplification and Sanger sequencing methods

To provide a Buxaceae Aquilaria plant Aquilaria sinensis[ Aquilaria sinensis ​ The application provides a fruit peel extract (PGB-B) of Aquilaria sinensis (Lour.) Spreng. and a pharmaceutical composition and a preparation method thereof, and application of the fruit peel extract in preparation of a medicine for treating psoriasis. The application belongs to the technical field of medicines. The fruit peel of dried Aquilaria sinensis is extracted by using water-containing ethanol, and PGB-B is prepared by using water solubility difference. In a mouse psoriasis model induced by imiquimod, after treatment by PGB-B, a psoriasis lesion area and a severity index (PASI) score are obviously reduced, skin thickness is reduced, and a spleen coefficient is reduced, which shows a significant treatment effect. PGB-B and different combinations thereof are new choices for treating psoriasis.

The application discloses a CDA enzyme-producing bacillus, and a culture method of the bacillus, which comprises the following steps: (1) using a fermentation medium containing MgSO4, (NH4)2SO4 and chitin to culture the CDA enzyme-producing bacillus for 168 hours; (2) using an LB medium to activate the CDA enzyme-producing bacillus, picking a single colony after the strain grows into a colony, inoculating the single colony into a new LB medium, and culturing for 2-3 days to obtain a seed liquid; (3) transferring the obtained seed liquid into a new fermentation medium to carry out large-scale culture, and carrying out low-speed centrifugation on the bacterial liquid after culturing for 3 days; and (4) pouring out part of the culture medium to obtain a concentrated bacterial liquid. The bacterial liquid can be used for promoting the linyang of agarwood. The CDA enzyme-producing bacillus obtained by purifying, fermenting and activating the bacillus GXU-B2 can be injected into the xylem of agarwood by means of liquid static drop, and the CDA enzyme and the bacillus can stimulate the defense system of the aquilaria plant to promote the linyang of agarwood, and the linyang is deep and uniform in the xylem.

The utility model relates to the technical field of cultivation devices, in particular to an aquilaria agallocha cultivation device. Comprising a main frame, a main fixed disc body is installed at the right end of the main frame, a lead screw is rotationally connected to the left side of the main frame, a movable frame is in threaded connection with the outer side of the lead screw, a main limiting rod is connected to the end of the movable frame, a main connecting rod is connected to the outer side of the main limiting rod, and a main movable disc body is installed at the end of the main connecting rod; and a main splicing rod is mounted at the end part of the main frame. According to the aquilaria agallocha cultivation device, a screw rod can rotate to drive a movable frame to move, a main connecting rod is controlled to drive a main movable disc body to move, and a spliced main limiting rod, an auxiliary limiting plate and an auxiliary limiting rod are utilized to synchronously drive an auxiliary connecting rod to drive an auxiliary movable disc body to move; the main movable disc body is separated from the main fixed disc body, the auxiliary movable disc body is separated from the auxiliary fixed disc body, and the agilawood seedlings with soil are taken out for transplanting under the condition that the roots are not damaged.

The invention discloses absidia GXU-F34 for promoting agilawood formation, the absidia GXU-F34 is obtained by intercepting a sample from an ancient tree and purifying and separating, and the absidia GXU-F34 can be used for promoting agilawood formation of aquilaria sinensis. The absidia GXU-F34 is a plant endophyte and can continuously generate some enzymes so as to stimulate a defense system of agilawood plants, a series of enzymes generated by saprophytic fungus microorganisms play an important role in a defense mechanism of the agilawood plants, and finally agilawood is generated.