The application discloses a CDA
enzyme-producing bacillus, and a culture method of the bacillus, which comprises the following steps: (1) using a
fermentation medium containing MgSO4, (NH4)2SO4 and
chitin to culture the CDA
enzyme-producing bacillus for 168 hours; (2) using an LB medium to activate the CDA
enzyme-producing bacillus, picking a single colony after the strain grows into a colony, inoculating the single colony into a new LB medium, and culturing for 2-3 days to obtain a seed liquid; (3) transferring the obtained seed liquid into a new
fermentation medium to carry out large-scale culture, and carrying out low-speed
centrifugation on the bacterial liquid after culturing for 3 days; and (4) pouring out part of the culture medium to obtain a concentrated bacterial liquid. The bacterial liquid can be used for promoting the linyang of
agarwood. The CDA enzyme-producing bacillus obtained by purifying, fermenting and activating the bacillus GXU-B2 can be injected into the
xylem of
agarwood by means of liquid static drop, and the CDA enzyme and the bacillus can stimulate the defense
system of the
aquilaria plant to promote the linyang of
agarwood, and the linyang is deep and uniform in the
xylem.