305 results about "Aquilaria sinensis" patented technology

The invention belongs to biological technical field, in particular to a liquid infusion method for producing linaloe on aquilaria sinensis trees. The method includes of drilling on the aquilaria sinensis trunk, gradually dropping a linaloe catalyst into the aquilaria sinensis tree body through the liquid infusion method; the operation is repeated every 2-3 months, after 6-12 months, the chocolatebrown oil-like material and the yellowish-brown discolored eaglewood formed in the trunk is cut and is dried to obtain the linaloe; the linaloe catalyst is mixed up by a phytohormone solution and a chemical reagent solution; the chemical reagent is ferric salt or sodium salt. The process of the invention has simple technique, convenient operation, can greatly increase production quantity of the linaloe and greatly shorten the production period of the linaloe through the inducing method, has good linaloe-forming effect, can effectively ease the contradiction between supply and demand for sinensis, is applicable to large-scale standardization and commercialization production, provides a new effective path for protecting, developing and utilizing the aquilaria sinensis tree resources, and has important economical, social and ecological benefits.

The invention relates to the field of biotechnology and discloses an agilawood production method. The method comprises the steps of first drilling a hole in a trunk of aquilaria malaccensis or aquilaria sinensis, enabling an agilawood inducer formed by plant hormones, inorganic salt and phenethyl alcohol to be added dropwise into the tree in a transfusion mode, then sealing the hole, adding the agilawood inducer dropwise at an interval of 1-2 months, performing treatment for 3-24 months in total, then cutting brown oily matter and yellowish-brown discolored xylem in the trunk, and obtaining agilawood after drying in the sun. The plant hormones are one or more than two of methyl jasmonate, ethephon and 6-BA; and the inorganic salt is one or both of sodium chloride and magnesium chloride. Components of the agilawood inducer are adjusted on the basis of an existing agilawood inducer, a trunk defense system is excited, related enzymes for synthesizing agilawood chemical components are generated and act on phenethyl alcohol, the trunk generates related chemical components in the agilawood, and finally the agilawood is formed by accumulation. According to the method, not only are contents of agilawood oil and sesquiterpenes in the agilawood oil improved, but also contents of alcohol-soluble extracts are improved.

An artificial induction agarwood production method from a tree of Aquilaria sinensis (Lour.) Gilg by high-pressure injection includes: inducer solution is instantly injected into a trunk of the tree of Aquilaria sinensis (Lour.) Gilg, the inducer solution is led to the tip of the tree through conducting tissues of the tree, the tree of tree of Aquilaria sinensis (Lour.) Gilg rejects drugs under stimulation of inducer and starts defense reaction to generate secondary metabolites, and finally agarwood formation is realized in the trunk under induction. The artificial induction agarwood production method is simple in process and convenient in operation, use of a high-pressure truck injector greatly shortens injection time of the inducer solution, and accordingly the operation is complete in one to two minutes and insusceptible to weather and wind force. Agarwood formation is effective, large-scale standard production is facilitated, and important economic benefit and great ecological benefit on protection of tree of Aquilaria sinensis (Lour.) Gilg and development and production of agarwood are achieved.

A making process of tea with leaves of Aquilaria sinensis includes the steps: firstly, selecting fresh leaves; secondly, sun-drying the fresh leaves; thirdly, air-drying and cooling the leaves by moving the fresh leaves of Aquilaria sinensis and the fresh tea leaves subjected to sun-drying to a cooling rack; fourthly, moving the leaves of Aquilaria sinensis and the tea leaves which are air-dried and cooled into a green-making room, mixing the leaves of Aquilaria sinensis and the tea leaves according to the mass ratio of 5-40% to 60-95%, and performing green-making and fermentation; fifthly, performing rolling and pan-frying; sixthly, rolling for shaping tea; and seventhly, baking to extract fragrance. The leaves of Aquilaria sinensis and the tea leaves are put together on the cooling rackfor air-drying and cooling, flavor of the leaves of Aquilaria sinensis and flavor of the tea leaves are allowed to permeate into each other, the leaves of Aquilaria sinensis and the tea leaves are then mixed proportionally for green-making and fermentation, the leaves of Aquilaria sinensis and the tea leaves are subjected to fermentation and fragrance extraction together, and accordingly the leaves of Aquilaria sinensis and the tea leaves are mutually and fully permeated and merged, and the made tea with leaves of Aquilaria sinensis has the effects of digestion aiding without harming stomach,and has strong long-lasting scent.

The invention discloses a tambac inducer and a preparation method thereof. The tambac inducer comprises an aquilaria sinensis tree vivo stem, a PDA culture medium, a potato sucrose culture medium and an aquilaria sinensis matrix. The method comprises the following steps of cutting off a part of an aquilaria sinensis tree vivo stem, which is forming tambac, and sterilizing, culturing, separating and identifying to obtain a tambac inducible strain; activating the strain by the PDA culture medium and fermenting by the potato sucrose liquid culture medium, and then inoculating the strain to the aquilaria sinensis matrix, and culturing for 3-5 days under the conditions of constant temperature of 30 DEG C, darkness and ventilation, thus obtain the tambac inducer. The invention has simple method, can remarkably shorten the forming time of the tambac, enhance the cultivating benefit of aquilaria sinensis trees and has favorable market application prospect.

The invention relates to a compound Chinese eaglewood tea and a manufacturing process thereof. The compound Chinese eaglewood tea is prepared by aquilaria sinensis leaf and stevia rebaudian leaf according to the mixture ratio of 1:9 to 9:1, and the manufacturing process comprises washing, tedding, drying green, piling, rolling, sterilizing, drying and other steps. The invention has the advantages that the stevia rebaudian leaf contains a large quantity of glycosides with high sweetness and low calorie and can neutralize the bitter taste of the aquilaria sinensis leaf without increasing the calorie and the sugar burdens of people who drink the compound Chinese eaglewood tea. Furthermore, the invention adopts the piling process for treating the aquilaria sinensis leaf during the manufacturing process, thereby reducing the bitter taste of the aquilaria sinensis leaf. The compound Chinese eaglewood tea manufactured by the invention has comfortable taste and is easy to be accepted by the people who drink the compound Chinese eaglewood tea.

The invention relates to an extraction method of agilawood essential oil, which comprises the following steps: firstly, cutting down aquilaria sinensis trees, removing white wood and carving into agilawood slices; grinding the agilawood slices into agilawood powder with specification of 40 meshes; then adding the agilawood powder into an extraction kettle by normal butane and directly extracting agilawood essential oil by adopting a subcritical fluid extraction technology under the extraction conditions that extraction time is 0.5 to 1.5 hours, analysis temperature is 60 DEG C, extraction temperature is 50 DEG C and extraction pressure is 0.4 Mpa to 0.5MPa; and then adding slags obtained after extracting the agilawood essential oil for the first time into the extraction kettle by 95 percent of ethanol to carry out extraction for the second time under the same extraction conditions in the process of extracting the agilawood essential oil for the first time. The invention aims to provide the extraction method and application of the agilawood essential oil.

The invention discloses a method for manually inducing Aquilaria sinensis to generate agilawood. The method comprises the following steps of: injecting a methanoic acid or acetic acid solution with pH value of 1.5-3 or a mixed solution of methanoic acid and acetic acid into xylem of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg]; respectively injecting the solution together with fungi, suchas Botryosphaeria rhodina, Hypocrea jecorina, Fusarium sp., Colletotrichum gloeosporioides, Ampelomyces sp, Khuskia sp, Fungal endophyte sp.A 16, Pestalotiopsis sp., Hypocrea lixii and Chaetomium sp.or injecting the solution and the fungi in a mixed way; and inducing the Aquilaria sinensis to generate the agilawood. According to the invention, the methanoic acid or acetic acid solution or the mixed solution of methanoic acid and acetic acid is injected into the xylem of the Aquilaria sinensis to induce the Aquilaria sinensis to generate defense reaction so as to generate the agilawood; and alarge amount of agilawood are generated by using the method. The method for manually inducing the Aquilaria sinensis to generate the agilawood, disclosed by the invention, has the advantages of simplicity and convenience in operation, low cost and suitability for manually making agilawood on a large scale by using Aquilaria sinensis trees; and a large amount of agilawood can be effectively generated by the Aquilaria sinensis. According to the invention, the agilawood producing period of the Aquilaria sinensis is largely reduced, the agilawood can be generally obtained after 6-24 months, the production cost is reduced, the market requirements are satisfied, and important economic, social and ecological benefits are obtained in aspects of protection and sustainable utilization of the Aquilaria sinensis, economic development of mountainous regions and the like.

The invention discloses a fungus for promoting aquilaria plants to generate agilawood and application of the fungus. The fungus is Fomitopsis sp. ASAF01, and the preservation number in the China General Microbiological Culture Collection Center is CGMCC NO.7802. The Fomitopsis sp.ASAF01 can promote the heartwood formation of aquilaria sinensis after the Fomitopsis sp. ASAF01 is inoculated for six months, and the middle of the formed agilawood is not easy to rot. Furthermore, the strain can still grow at the temperature of 15-40 DEG C and pH value of 2-11 to indicate that the Fomitopsis sp.ASAF01 has strong environmental tolerance.

The invention discloses an aquilaria sinensis fruit skin extract, a preparation method thereof and application thereof. The method comprises the following steps of: crushing aquilaria sinensis fruit skin serving as a raw material, adding water, ethanol, ethanol water, ethyl acetate, trichloromethane or petroleum ether serving as a solvent, performing reflux extraction, ultrasonic extraction or cold extraction to obtain extracting solution, and performing concentration drying under reduced pressure or freeze drying on the extracting solution to obtain the aquilaria sinensis fruit skin extract. The aquilaria sinensis fruit skin extract has broad-spectrum antimicrobial activity, so that the extract can be applied to a food preservative and the preparation of antibacterial medicaments or antifungal medicaments. The aquilaria sinensis fruit skin extract has a simple preparation method, low costs and abundant raw materials. Therefore, the utilization rate and economic benefit of aquilaria sinensis are improved.

The invention discloses application of an aquilaria sinensis peel extract in preparation of whitening cosmetics. The aquilaria sinensis peel extract is prepared by a method comprising the following steps of: by taking aquilaria sinensis peel as the raw material, extracting the aquilaria sinensis peel through ethyl acetate; and drying an extracting solution to obtain the aquilaria sinensis peel extract. The aquilaria sinensis peel extract has an inhibition effect on tyrosinase and can be used for preparing a tyrosinase inhibitor in order to be applied to the whitening cosmetics.

The invention provides agilawood sesquiterpenoid synthase protein ASS4 and an encoding gene ASS4 thereof. The protein comprises the following components: (1), protein formed by amino acid sequence shown in SEQ ID NO.2; (2), protein derived from the protein in (1), having the same function as the protein in (1), and formed in the way that one or more amino acids in the amino acid sequence shown in SEQ ID NO.2 are replaced and deleted or added. The invention further provides a preparation method and application of the agilawood sesquiterpenoid synthase protein ASS4. The agilawood sesquiterpenoid synthase protein ASS4, provided by the invention, can be used for catalyzing agilawood sesquiterpenoid externally, the gene ASS4 provided by the invention can be converted into prokaryotic cell, yeast or more advanced eukaryocyte, so as to have corresponding agilawood sesquiterpenoid synthase activity, and the mass production of agilawood sesquiterpenoid can be realized, and the damage to the resource of rare and endangered South China medical aquilaria sinensis is reduced, therefore, the agilawood sesquiterpenoid synthase protein ASS4 has significant economic value and application prospect.

The invention discloses high-purity mangiferin prepared from leaves and twigs of aquilaria sinensis and a preparation method thereof. The method comprises the following steps: on the basis of utilizing leaves and/or twigs of aquilaria sinensis as raw materials, extracting with an alcohol solution; after recovering alcohol at reduced pressure, carrying out water precipitation, alcohol precipitation, devitrification and the like so as to obtain a mangiferin crude product; and then recrystallizing to prepare the high-purity mangiferin. The method has the advantages that the leaves and twigs of aquilaria sinensis are used as the raw materials; the content of the mangiferin is high, and the variety and quantity of impurities are both low; the obtained high-purity mangiferin dose not contain allergic components, and the final product is high in quality and can be widely applied to the industries such as medicine, health care products and cosmetics; and the method has the advantages of simple, stable, practicable preparation process step, short production period, less labor consumption and low cost.

The invention discloses a method for separating active ingredients from an aquilaria sinensis lamina by utilizing a high-speed counter current chromatography. The method comprises the following steps of: extracting the dried and smashed aquilaria sinensis lamina by virtue of a percolation extract method at the room temperature to obtain an aquilaria sinensis lamina extracting solution extractum; extracting the extracting solution extractum for 3-4 times by sequentially utilizing petroleum ether,and ethyl acetate, respectively decompressing and concentrating a petroleum ether extract liquor and an ethyl acetate extract liquor to extractums; then respectively separating the petroleum ether part extractum and the ethyl acetate part extractum to obtain an active ingredient A of 7,4'-dimethoxy-5-hydroxy flavone by utilizing the high-speed counter current chromatography; and then separating the ethyl acetate part extractum through high-speed countercurrent chromatography to obtain an active ingredient B of 3,5,7,3',4'-pentahydroxy-flavone and an active ingredient C of 3,5,7,4'-tetrahydroxy-flavone after coarsely separating the ethyl acetate part extractum by virtue of a column chromatography on silica gel. The active ingredients separated by the method are low in loss, high in separation speed, high in purity, stable and easy to apply.

The invention relates to a compound and method for stimulating overall coagulation of aquilaria sinensis. According to the compound for stimulating overall coagulation of the aquilaria sinensis, biological inducing liquid prepared from aquilaria sinensis white mycelia extracts, auximone and a penetration enhancer is injected into an aquilaria sinensis plant, by means of the penetration enhancing function of the penetration enhancer, the compound is distributed on the xylem and other parts in a tree trunk more sufficiently, the inducing liquid extends to the treetop from the root of the tree trunk by means of the dredging function of the plant, the aquilaria sinensis is induced to start a coagulation mechanism, after accumulation, agilawood is formed in the aquilaria sinensis tree trunk, after six months of induction, the overall coagulation effect (including the xylem) can be achieved through induction, and the content of formed linalool extractum meets the standards of the national pharmacopoeia.

The invention provides a microcapsule for promoting Aquilaria sinensis trees or thymelaeaceae trees to produce agilawood. The microcapsule is wrapped with agilawood inducer, and the agilawood inducer is prepared from the following components in parts by weight: 10-30 parts of plant hormone, 50-75 parts of inorganic salt and 5-15 parts of phenethyl alcohol. The invention also provides a preparation method of the microcapsule and a method for promoting agilawood formation of the Aquilaria sinensis tree or the thymelaeaceae tree. According to the method for producing agilawood, a condition similar to the natural agilawood-producing condition can be acquired, little tree damage is caused, the trees can be subjected to agilawood formation again after agilawood collection, and the yield and the quality of the formed agilawood are effectively improved.

The invention belongs to the technical field of biology and relates to a method for cultivating agilawood dendrobium officinale. The method for cultivating the agilawood dendrobium officinale is characterized by comprising the following steps of: 1, drilling holes at a slanting downward angle of 20 degrees at the positions on an agilawood living trunk at a tree age of 8 to 10 years, which are at a distance of 30 to 50cm from the ground, wherein the hole diameter is 2cm and the hole depth is 5cm; 2, transplanting dendrobium officinale seedlings into the drilled tree holes and simultaneously adding a culture medium, wherein the culture medium comprises 20 to 30g/L of Chinese herb residue medium, 35 to 80g/L of peat, 20 to 33kg/L of vermiculite, 1 to 2g/L of urea, 1 to 3g/L of superphosphate, 1 to 3g/L of potassium chloride and 1 to 3mg/L of 6-BA, and the planting interval is 15 to 20cm; and 3, winding a plastic thin film on the dendrobium officinale and the agilawood trunk, wherein the width of the plastic thin film is 3 to 5cm and the number of wound turns is 2 to 3 turns. According to the invention, the culture medium is optimized, so that the dendrobium officinale has a high survival rate in aquilaria sinensis; and meanwhile, an aquilaria sinensis tree is induced to bear agilawood.

The invention discloses special organic fertilizer for Hainan fragrant rosewood. The organic fertilizer comprises raw materials in parts by weight as follows: seafood waste, animal dung, tea waste, egg shells, soya bean meal, attapulgite, chillies, bread crumbs, alpha-amino acid, a molasses fermentation broth, oilseed rape shells, loofah sponges, corn stigma, peanut coat, winter bamboo shoot shells, corn bran, walnut shells, hazelnut shells, peanut hulls, ginger peel, coconut shells, durian shells, longan shells, litchi shells, garlic skin, cactuses, areca nuts, Japanese dock root, chinaberry tree bark, pumpkin seeds, willowleaf swallowwort rhizome, rhizoma arisaematis, divaricate saposhnikovia root, solomonseal rhizome, , benzoin, aquilaria sinensis, villous amomum fruit, panax notoginseng, wolfiporia extensa, sophora flavescens, mint and Chinese pulsatilla root. The invention further discloses a preparation method of the special organic fertilizer for the Hainan fragrant rosewood. The organic fertilizer for the Hainan fragrant rosewood contains rich organic matters, improves the drought resistance, cold resistance and disease resistance, and remarkably increases the seedling height, the ground diameter, the root length and the biomass.

The invention discloses an agarwood promoter and method for producing agarwood in Aquilaria sinensis. It is characterized in that the agarwood promoter is composed of following components in volume percentage: 5-10% of liquid cultivating Schizophyllum commune, 1.5-2% glucose or granulated sugar, 0.5-1% of acetate or malic acid, 85-95% of water. The method comprises the following steps: perforating Aquilaria sinensis trunk at a place not higher than 40cm through the bark, cambium, then perforating the secondary xylem, but hot hurting the primary xylem; introducing the agarwood promoter into the hole using a conduit and agarwood can be formed in half a year. The agarwood promoter of the invention has simple components and is easily acquired and prepared; the operation of the agarwood promoter to promote agarwood production is simple; the method of the invention has many advantages: obvious effect for effectively increasing agarwood productivity, shortening agarwood production period, better utilization and protection for Aquilaria sinensis resources.

The invention belongs to the field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine oral administration and external application combined preparation. The traditional Chinese medicine oral administration and external application combined preparation comprises an oral administration medicine and an external application medicine, wherein the oral administration medicine contains rhizoma drynariae, angelica sinensis, frankincense, pseudo-ginseng, sangusis draconis, Chinese polyphaga, myrrh, native copper, trogopterus dung, dipsacales, aquilaria sinensis, bitter orange, pawpaw, salvia miltiorrhiza bunge, large leaf gentian root, peach kernel, earthworm, cortex acanthopanacis, radix achyranthis bidentatae, eucommia bark, glaucousback threewingnut root bark, cassia twig, astragalus mongholicus, radix angelicae, rhizoma corydalis, pericarpium citri reticulatae viride, cortex moutan, rhizoma cibotii, bupleurum falcatum, beautiful sweetgum fruit, combined spicebush root, divaricate saposhnikovia root, almond, agastache rugosus, cimicifugae foetidae, betel nut, radix angelicae pubescentis, notopterygium root, Szechuan lovage rhizome, complete blood, madder, coralbean bark, safflower and sinomenium acutum; and the external application medicine contains centipede, chilopod wall gecko, radix angelicae, myrrh, safflower, giant typhonium rhizome, snow mountain wormwood, borneol, glaucousback threewingnut root bark, radix aconiti agrestis, dragon blood, camphor, sundew, complete blood, frankincense, angelica sinensis, rhizoma arisaematis and pinellia ternate. The traditional Chinese medicine oral administration and external application combined preparation of the invention can effectively treat diseases, such as osteoproliferation, and the like, and the disease is difficult to recur after being healed.

The invention provides a method for optimizing extraction technology of aquilaria sinensis leaf tea polyphenol by utilizing a response surface process. The method comprises the following steps: 1) preparing a test liquid; 2) determining the content of tea polyphenol in the extraction liquid; 3) performing experiment design and statistics analysis: a) performing single-factor experiment; and b) optimizing the design through the response surface process, concretely, taking the single-factor experiment as the basis, selecting extraction time, liquid-material ratio, extraction temperature and ethanol concentration as response variables, taking the aquilaria sinensis leaf tea polyphenol content as the response value, and employing the response surface process to optimize the aquilaria sinensis leaf tea polyphenol extraction conditions; and 4) analyzing and optimizing the experiment results, concretely, performing calculation on the regression equation through a Design-Expert. 8.05 software, so as to obtain the optimum technological conditions for extracting aquilaria sinensis leaf tea polyphenol.

The invention discloses the application of supercritically-extracted aquilaria sinensis in the tobacco remanufacturing technology with the paper making method. The specific preparation technology comprises the first step of extraction, wherein tobacco raw materials are mixed and added with water to be extracted; the second step of separation, wherein after extraction is finished, extracted liquid and solid raw material residues are obtained through solid-liquid separation; the third step of aquilaria sinensis supercritical extraction, wherein dried and meshed aquilaria sinensis is extracted through the supercritical carbon dioxide fluid to obtain extracts and residues, the obtained extracts are dispersed by absolute ethyl alcohol and then blended into the extracted liquid obtained in the second step, the mixture is concentrated to manufacture coating liquid, the obtained residues are blended into the solid raw material residues obtained in the second step, mixing and pulping are conducted, and tobacco piece bases are manufactured through the paper manufacturing technology with pulp; the fourth step of coating, wherein the tobacco piece bases are coated with the coating liquid, and remanufactured tobacco products are manufactured through drying and cutting. The preparation technology is simple, the cost is low, limited aquilaria sinensis resources are effectively utilized, and the sensory quality of aquilaria sinensis cigarettes is improved.

The invention discloses a method for separating and purifying mangiferin from aquilaria sinensis leaves. The method is used for solving the problems such as low purity, low yield and complex operation of the mangiferin separated by virtue of the existing process. The method comprises the following step: quickly preparing the high-purity mangiferin by use of the aquilaria sinensis leaves used as raw materials by virtue of adopting a method of combining normal phase column chromatography with high-speed counter-current chromatography. The method has the advantages of great preparation amount, high efficiency, quickness, low cost, simplicity in operation, high product quality and no pollution; and moreover, a solvent can be recycled, the solvent cost and the solvent treatment cost can be lowered, and the method can be widely applied to industrial production.

The invention relates to the field of line incense and specifically relates to an agilawood line incense and a preparation method thereof. The line incense comprises the following raw materials in parts by weight: 60 to 80 parts of agilawood powder, 3 to 10 parts of aquilaria sinensis leaf powder, and 10 to 20 parts of food grade adhesive. The agilawood line incense is mainly composed of agilawood and has a fragrance. The health care functions of agilawood such as heart calming, tranquilizing, qi absorbing, pant relieving, qi moving, blood activating, and the like, are fully exerted. The line incense is natural and healthy and is harmless for the human body. Due to the food grade adhesive, after the line incense is burned, the ash can fall down easily and will not influence the burning of the line incense, moreover, the line incense has a stable structure before burning, the line incense also comprises aquilaria sinensis leaves, the fragrance is more pleasant, and aquilaria sinensis leaf has the functions of soothing the nerves and relieving fatigue and is well matched with agilawood.