Application of burkholderia gladioli XC-1 in prevention and treatment of plant diseases

Abstract

The invention discloses an application of Burkholderia gladioli XC-1 in prevention and treatment of plant diseases, the 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3, and the plant diseases are litsea cubeba shoot blight, okra root rot, durian leaf spot disease, cubilose fruit canker, agilawood root rot or cassava leaf spot disease. The invention finds that the Burkholderia gladioli XC-1 has better development value and application potential in the prevention and treatment of various plant diseases, and a new microbial strain resource is provided for the prevention and treatment of various plant diseases.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbiology. Specifically, the present invention relates to the application of a strain of Burkholderia gladioli XC-1 in preventing and controlling various plant diseases. Background Art

[0002] In recent years, due to changes in the global climate environment, adjustments in farming practices, and interference from human factors, the incidence of plant diseases has been continuously rising, the degree of harm has been increasing, and the difficulty of prevention and control has also been increasing day by day. Plant diseases not only directly threaten food security and crop quality, but also have a serious impact on crop yields, economic stability, and the ecological environment, becoming a difficult problem that urgently needs to be solved in the global agricultural field.

[0003] Currently, chemical fungicides are still widely regarded as the most effective means of prevention and control. To ensure agricultural production, their usage has been increasing year by year, and even the phenomenon of overuse has occurred. However, over-reliance on chemical pesticides has brought many negative impacts: First, pesticide residues pollute the ecological environment, damage biodiversity, and lead to ecological imbalance; second, pesticides remaining on agricultural products threaten food security and endanger human health; third, long-term and large-scale use of chemical fungicides may enhance the drug resistance of pathogenic bacteria, further increasing the difficulty of disease prevention and control.

[0004] Facing this severe challenge, reducing the reliance on chemical pesticides and promoting green prevention and control technologies have become the top priority. As an innovative means in the modern plant pest and disease prevention and control system, biological control technology is playing an increasingly important role in the sustainable development of agriculture. This technology realizes the ecological prevention and control of plant pests and diseases by developing and utilizing beneficial microorganisms and their metabolites in the environment or plant tissues, and has significant advantages such as environmental friendliness, high safety, and low resistance generation. Compared with traditional chemical control, biological control can not only effectively control diseases, but also promote plant growth, improve crop yields and quality, and maintain ecological balance while ensuring the safety of agricultural products.

[0005] In the past few decades, with the rapid development of biotechnology, significant progress has been made in the research and application of beneficial microorganisms such as Bacillus sp., Pseudomonas sp., Streptomyces sp., and Trichoderma sp. These microorganisms play a disease-preventing role through various mechanisms such as producing antibiotics and inducing systemic resistance, and have been widely used in the prevention and control of diseases of various crops. However, there are still many gaps in the research on biological control of specific diseases of specific plants. Summary of the Invention

[0006] Based on this, the object of the present invention is to provide the application of a strain of Burkholderia gladioli XC-1 in preventing and controlling various plant diseases.

[0007] The specific technical solutions for achieving the above object of the invention are as follows.

[0008] In the first aspect of the present invention, there is provided the application of a strain of Burkholderia gladioli XC-1 in preventing and controlling plant diseases or preparing a bacterial agent for preventing and controlling plant diseases. The 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3, and the plant diseases are Litsea cubeba dieback, okra damping-off, durian leaf spot, pitaya canker, Aquilaria root rot or cassava leaf spot.

[0009] In the second aspect of the present invention, there is provided the application of a strain of Burkholderia gladioli XC-1 in inhibiting plant disease pathogens or preparing a bacterial agent for inhibiting plant disease pathogens. The 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3, and the pathogens are Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae or Diaporthe ueckeri.

[0010] In the third aspect of the present invention, there is provided the application of a bacterial agent in preventing and controlling plant diseases or inhibiting plant disease pathogens. The bacterial agent is Burkholderia gladioli XC-1, its bacterial suspension or fermentation broth; the 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3; the plant diseases are Litsea cubeba dieback, okra damping-off, durian leaf spot, pitaya canker, Aquilaria root rot or cassava leaf spot; the pathogens are Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae or Diaporthe ueckeri.

[0011] In a fourth aspect of the present invention, a method for preventing and controlling plant diseases is provided, comprising the following steps: spraying a bacterial suspension or fermentation liquid of Burkholderia gladioli XC-1 on plants, wherein the 16S rRNA sequence of Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3, and the plant is litsea cubeba, okra, durian, bird's nest fruit, agarwood or cassava.

[0012] The invention discloses a Burkholderia gladioli strain XC-1 isolated from an agarwood trunk, which is effective against Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae, and Diaporthe The results of field tests showed that XC-1 had a significant control effect on litsea cubeba dieback, so XC-1 has good development value and application potential in the prevention and control of various plant diseases such as litsea cubeba dieback, okra wilt, cassava leaf spot, agarwood root rot, bird's nest fruit canker, and durian leaf spot, providing a new microbial strain resource for the prevention and control of various plant diseases.

[0013] The Burkholderia XC-1 described in the present invention is classified as Burkholderia gladioli, and was deposited in the Guangdong Provincial Microbiological Culture Collection Center on January 8, 2025. The deposit address is Building 59, No. 100 Xianlie Middle Road, Guangzhou, and the deposit number is GDMCC No: 65641. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a plate confrontation experiment diagram of strain XC-1 in Example 1 of the present invention and different plant pathogens; A to F are pathogens Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae and Diaporthe ueckeri.

[0015] Figure 2This is the colony morphology diagram of strain XC-1 in PDA medium in Example 2 of the present invention.

[0016] Figure 3 This is the phylogenetic tree diagram of strain XC-1 based on 16S rRNA sequence analysis in Example 2 of the present invention.

[0017] Figure 4 This is the symptom diagram of twig blight of Litsea cubeba seedlings sprayed with clear water in Example 3 of the present invention.

[0018] Figure 5 This is the field control effect diagram of strain XC-1 on twig blight of Litsea cubeba in Example 3 of the present invention, where A is the group sprayed with clear water and B is the group sprayed with XC-1 fermentation broth. Detailed implementation manners

[0019] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0020] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not used to limit the present invention. The term "and / or" used in the present invention includes any and all combinations of one or more of the related listed items.

[0021] If not otherwise specified, the embodiments are all carried out under conventional experimental conditions, such as in the Molecular Cloning experimental manual by Sambrook et al. (Sambrook J & Russell DW, Molecular Cloning: a Laboratory Manual, 2013), or according to the conditions recommended by the manufacturer's instructions.

[0022] In some embodiments of the present invention, the application of a Burkholderia gladioli strain XC-1 in controlling plant diseases is disclosed. The 16S rRNA sequence of the Burkholderia gladioli strain XC-1 is as shown in SEQ ID NO: 3, and the plant diseases are twig blight of Litsea cubeba, damping-off of okra, leaf spot of durian, canker of pitaya, root rot of Aquilaria sinensis, or leaf spot of cassava.

[0023] In some other embodiments of the present invention, the application of a strain of Burkholderia gladioli XC-1 in the preparation of a microbial agent for preventing and controlling plant diseases is disclosed. The 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO:3, and the plant diseases are Litsea cubeba twig blight, okra damping-off, durian leaf spot, pitaya canker, Aquilaria root rot or cassava leaf spot.

[0024] In some other embodiments of the present invention, the application of a strain of Burkholderia gladioli XC-1 in inhibiting plant disease pathogens is disclosed. The 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO:3, and the pathogens are Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae or Diaporthe ueckeri.

[0025] In some other embodiments of the present invention, the application of a strain of Burkholderia gladioli XC-1 in the preparation of a microbial agent for inhibiting plant disease pathogens is disclosed. The 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO:3, and the pathogens are Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae or Diaporthe ueckeri.

[0026] In some other embodiments of the present invention, an application of a bacterial agent in preventing and controlling plant diseases or inhibiting plant disease pathogens is disclosed. The bacterial agent is a bacterial suspension or fermentation broth of Burkholderia gladioli XC-1, the plant diseases are Litsea cubeba twig blight, okra damping-off, durian leaf spot, pitaya canker, Aquilaria root rot or cassava leaf spot, and the pathogens are Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae or Diaporthe ueckeri.

[0027] In some other embodiments of the present invention, a method for preventing and controlling plant diseases is disclosed, which includes the following steps: spraying the above-mentioned bacterial agent on plants, and the plants are Litsea cubeba, okra, durian, pitaya, Aquilaria or cassava.

[0028] In some of the embodiments, the bacterial agent is a fermentation broth of Burkholderia gladioli XC-1, and the concentration of Burkholderia gladioli XC-1 in the fermentation broth is 0.8×10 7 cfu / mL to 1.2×10 7 cfu / mL.

[0029] In some of the embodiments, the spraying sites are the branches, leaves and / or fruits of the plants.

[0030] In some of the embodiments, spraying is carried out once every 25 days to 35 days, and a total of 2 to 3 times of spraying are carried out.

[0031] In some other embodiments of the present invention, a method for preventing and controlling Litsea cubeba twig blight is disclosed, which includes the following steps: spraying the above-mentioned bacterial agent on Litsea cubeba seedlings or plants.

[0032] In the following examples, the tested plant pathogens Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae and Diaporthe ueckeri were all isolated from the diseased parts of plants by the applicant in the early stage, stored in the laboratory, and can also be obtained commercially.

[0033] The present invention will be described in detail below with reference to the accompanying drawings and specific examples.

[0034] Example 1 Isolation of Bacterium XC-1 and Its Inhibitory Effect on Different Plant Pathogens

[0035] In this example, a strain of bacterium XC-1 was isolated and screened by the dilution coating plate method and the plate confrontation method, and it was found that it has an inhibitory effect on a variety of plant pathogens. The specific steps are as follows:

[0036] 1. Use a sterilized blade to shave off the epidermis of the collected Aquilaria sinensis tree trunk, cut small pieces of tissue (5×5 mm), place them in 1 mL of sterilized water, shake well, take 100 μL of the solution and spread it on a fresh PDA medium. After single colonies grew on the plate, pick monoclonal colonies for purification. A total of 1 strain of bacterium (named XC-1) was obtained, and the rest were Trichoderma spp.

[0037] 2. Mark on the back of the PDA plate by the cross method, use a sterilized punch with a diameter of 6 mm to prepare agar discs of the pathogens of Litsea cubeba twig blight (Cophinforma tumefaciens), okra damping-off (Rhizoctonia solani), durian leaf spot (Colletotrichum sp.), pitaya canker (Neoscytalidium dimidiatum), Aquilaria root rot (Lasiodiplodia theobromae) and cassava leaf spot (Diaporthe ueckeri), and inoculate the agar discs in the center of the PDA plate medium.

[0038] The cells of strain XC-1 cultured overnight at 28 °C were centrifuged and washed twice with sterile water, and then the concentration was adjusted to OD 600 1.0 bacterial suspension with sterile water for standby.

[0039] Mark 4 points on the cross line 2.5 cm away from the agar disc. Among them, 3 points were inoculated with 2 μL of the bacterial suspension of XC-1 (experimental group), and 1 point was not inoculated. Each treatment was repeated 3 times.

[0040] When the colonies of each pathogen (control group) grew close to the edge of the culture dish, measure the colony size, and calculate the inhibition rate according to the formula.

[0041] Inhibition rate (%) = (diameter of control group - diameter of experimental group) / diameter of control group × 100

[0042] 3. The results of the plate confrontation experiment are shown in Table 1 and Figure 1 as follows.

[0043] Table 1 Inhibitory Effect of Strain XC-1 on Different Plant Pathogens

[0044]

[0045] As can be seen from Table 1 and Figure 1 it can be seen that the isolated bacterium XC-1 has good antagonistic effects against the pathogens of Litsea cubeba blight (Cophinforma tumefaciens), okra damping-off (Rhizoctonia solani), durian leaf spot (Colletotrichum sp.), pitaya canker (Neoscytalidium dimidiatum), agarwood root rot (Lasiodiplodia theobromae), and cassava leaf spot (Diaporthe ueckeri). The inhibition rates are 75.70%, 71.11%, 70.42%, 74.07%, 71.11%, and 72.22% in sequence, and the inhibition effect on the pathogen Cophinforma tumefaciens of Litsea cubeba blight is the best.

[0046] Example 2 Identification of Bacterium XC-1

[0047] The colony of strain XC-1 on PDA medium is light yellow, the surface of the colony is moist, and the edge is irregular ( Figure 2 ).

[0048] Using the universal primers 16S-27f (SEQ ID NO:1) / 16S-1492r (SEQ ID NO:2), the 16S rRNA gene fragment of XC-1 was amplified by colony PCR method.

[0049] 16S-27f: AGAGTTTGATCCTGGCTCAG (SEQ ID NO:1)

[0050] 16S-1492r: TACGGCTACCTTGTTACGACTT (SEQ ID NO:2)

[0051] After being detected positive by 1% agarose gel electrophoresis, it was recovered and sent to the company for sequencing. The obtained sequence was subjected to Blastn analysis. The results of Blastn analysis of the 16S rRNA sequence showed that the strain XC-1 had high sequence similarity with multiple species such as Burkholderia gladioli, Burkholderia glumae, Burkholderia rinojensis, Burkholderia plantarii, Burkholderia perseverans, Burkholderia stagnalis, Burkholderia stabilis, Burkholderia cepacia, Burkholderia pyrrocinia, Burkholderia arboris, Burkholderia multivorans, Mycetohabitans endofungorum, Burkholderia lata, Burkholderia puraquae, Burkholderia latens, Burkholderia ambifaria, Burkholderia ubonensis, Burkholderia vietnamiensis. The type strains of the species with high homology to the strain XC-1 were selected, and phylogenetic analysis was performed based on the 16S rRNA gene sequence. The results are as Figure 3 shown. Figure 3 The results showed that the strain XC-1 and Burkholderia gladioli were on the same evolutionary branch.

[0052] Therefore, based on the colony morphological characteristics of the strain XC-1 and the analysis results of the 16S rRNA gene sequence (as shown in SEQ ID NO:3), the strain XC-1 was preliminarily identified as Burkholderia gladioli.

[0053] SEQ ID NO:3:

[0054] GGCAGCACGGGTGCTTGCACCTGGTGGCGAGTGGCGAACGGGTGAGTAAT

[0055] ACATCGGAACATGTCCTGTAGTGGGGGATAGCCCGGCGAAAGCCGGATTA

[0056] ATACCGCATACGATCTACGGATGAAAGCGGGGGACCTTCGGGCCTCGCGCT

[0057] ATAGGGTTGGCCGATGGCTGATTAGCTAGTTGGTGGGGTAAAGGCCCACCA

[0058] AGGCGACGATCAGTAGCTGGTCTGAGAGGACGACCAGCCACACTGGGAC

[0059] TGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGAC

[0060] AATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGTGTGAAGAAGGCCTTC

[0061] GGGTTGTAAAGCACTTTTGTCCGGAAAGAAATCCTGAGGGCTAATATCCTT

[0062] CGGGGATGACGGTACCGGAAGAATAAGCACCGGCTAACTACGTGCCAGCA

[0063] GCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAA

[0064] GCGTGCGCAGGCGGTTTGTTAAGACCGATGTGAAATCCCCGGGCTCAACC

[0065] TGGGAACTGCATTGGTGACTGGCAAGCTAGAGTATGGCAGAGGGGGGTAG

[0066] AATTCCACGTGTAGCAGTGAAATGCGTAGAGATGTGGAGGAATACCGATG

[0067] GCGAAGGCAGCCCCCTGGGCCAATACTGACGCTCATGCACGAAAGCGTGG

[0068] GGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA

[0069] ACTAGTTGTTGGGGATTCATTTCCTTAGTAACGTAGCTAACGCGTGAAGTT

[0070] GACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGG

[0071] GGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAA

[0072] AACCTTACCTACCCTTGACATGGTCGGAACCTTGGAGAGATCCGAGGGTG

[0073] CTCGAAAGAGAACCGATACACAGGTGCTGCATGGCTGTCGTCAGCTCGTG

[0074] TCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTAGT

[0075] TGCTACGCAAGAGCACTCTAGGGAGACTGCCGGTGACAAACCGGAGGAA

[0076] GGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGGGTAGGGCTTCACACG

[0077] TCATACAATGGTCGGAACAGAGGGTCGCCAACCCGCGAGGGGGAGCTAAT

[0078] CCCAGAAAACCGATCGTAGTCCGGATTGCACTCTGCAACTCGAGTGCATG

[0079] AAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTC

[0080] CCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAG

[0081] AAG

[0082] Evaluation of the Field Control Effect of Strain XC-1 Against Litsea cubeba Dieback

[0083] The strain XC-1 was inoculated into LB medium and cultured with shaking at 28 °C and 200 rpm for 2 days to prepare the XC-1 fermentation broth. Subsequently, the fermentation broth was diluted with sterile water to a concentration of 1.0×10 7 cfu / mL, and 2% sucrose was added as an adjuvant for standby use.

[0084] Six diseased Litsea cubeba seedlings were selected as the treatment objects, and the number of disease spots on each Litsea cubeba seedling was counted and divided into two groups. The diluted XC-1 fermentation broth was evenly sprayed on the surface of the seedling branches once a month for a total of 2 sprays, with 1 L sprayed each time, as the experimental group. The control group was sprayed with an equal amount of sterile water. The experiment was carried out under natural conditions, and the change in the number of disease spots was observed regularly. One month after the two spraying treatments were completed, the development of the disease was counted to evaluate the control effect. The results showed that in the treatment group sprayed with the XC-1 fermentation broth, the number of disease spots increased from an average of 12 per plant to 15 per plant, with an increase rate of 20%; while in the control group, the number of disease spots increased from 12 per plant to 30 per plant, with an increase rate of 60%, and some plants even had dead tips at the top ( Figure 4 ). It can be seen that spraying the XC-1 fermentation broth on the diseased Litsea cubeba seedlings can enable it to colonize on the surface of the young trees, thereby occupying the living space of the pathogenic bacteria, inhibiting the growth of the pathogenic bacteria, reducing the survival chance of the pathogenic bacteria on the surface of the young trees, and thus showing that the chance of the young trees getting sick, that is, the number of disease spots formed is less than that of the young trees in the control group. Therefore, XC-1 has a certain control effect on the diseases of Litsea cubeba and can significantly inhibit the increase in the number of disease spots.

[0085] In addition, the XC-1 fermentation broth and water (3 plants each) were respectively sprayed on the wounds formed after the pathogenic bacteria invaded the diseased adult Litsea cubeba plants once a month, with 1 L sprayed each time for a total of 2 sprays. One month after the last spray was completed, it was found that for the Litsea cubeba plants sprayed with the XC-1 fermentation broth, since the growth of the pathogenic bacteria at the infected and cracked parts was inhibited, the pathogenic bacteria would not further damage the plant tissues, and thus the cracked parts healed through the plant's own repair mechanism, while the infected and cracked parts of the plants sprayed with water did not heal ( Figure 5 ).

[0086] The results of this example show that Burkholderia gladioli XC-1 has a significant control effect on the tip blight of Litsea cubeba and has application potential in the preparation of biological agents for controlling the tip blight of Litsea cubeba.

[0087] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification.

[0088] The above-described embodiments merely represent several implementation manners of the present invention. The description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the appended claims.

Claims

1. Use of a Burkholderia gladioli XC-1 strain in preventing and controlling plant diseases, characterized in that: The 16S rRNA sequence of the Burkholderia gladioli XC-1 is shown in SEQ ID NO: 3, and the plant disease is litsea cubeba dieback, okra blight, durian leaf spot, bird's nest fruit canker, agarwood root rot or cassava leaf spot.

2. Use of a Burkholderia gladioli XC-1 strain in the preparation of a bacterial agent for preventing and controlling plant diseases, characterized in that: The 16S rRNA sequence of the Burkholderia gladioli XC-1 is shown in SEQ ID NO: 3, and the plant disease is litsea cubeba dieback, okra blight, durian leaf spot, bird's nest fruit canker, agarwood root rot or cassava leaf spot.

3. Use of a Burkholderia gladioli XC-1 strain in inhibiting plant disease pathogens, characterized in that: The 16SrRNA sequence of the Burkholderia gladioli XC-1 is shown in SEQ ID NO: 3, and the pathogenic bacteria is Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae or Diaporthe ueckeri.

4. Use of a Burkholderia gladioli XC-1 strain in the preparation of a microbial agent for inhibiting plant disease pathogens, characterized in that: The 16S rRNA sequence of the Burkholderia gladioli XC-1 is shown in SEQ ID NO: 3, and the pathogenic bacteria is Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodiatheobromae or Diaporthe ueckeri.

5. Use of a bacterial agent in preventing and controlling plant diseases or inhibiting plant disease pathogens, characterized in that: The bacterial agent is a bacterial suspension or fermentation liquid of Burkholderia gladioli XC-1; the 16S rRNA sequence of the gladiolus Burkholderia gladioli XC-1 is shown in SEQ ID NO:3; the plant disease is litsea cubeba dieback, okra blight, durian leaf spot, bird's nest fruit canker, agarwood root rot or cassava leaf spot; the pathogen is Cophinforma tumefaciens, Rhizoctonia solani, Colletotrichum sp., Neoscytalidium dimidiatum, Lasiodiplodia theobromae or Diaporthe ueckeri.

6. A method for preventing and controlling plant diseases, characterized in that: The following steps are involved: A bacterial suspension or fermentation liquid of Burkholderia gladioli XC-1 is sprayed on plants, wherein the 16S rRNA sequence of Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3, and the plants are litsea cubeba, okra, durian, bird's nest fruit, agarwood or cassava.

7. The method for preventing and controlling plant diseases according to claim 6, characterized in that: The plants were sprayed with a fermentation broth of Burkholderia gladioli XC-1, wherein the concentration of Burkholderia gladioli XC-1 in the fermentation broth was 0.8×10 7 cfu / mL~1.2×10 7 cfu / mL.

8. The method for preventing and controlling plant diseases according to claim 6, characterized in that: The spraying sites are branches, seedlings, leaves and / or fruits of plants.

9. The method for preventing and controlling plant diseases according to claim 6, characterized in that: Spray once every 25 to 35 days, for a total of 2 to 3 times.

10. A method for preventing and treating litsea cubeba dieback disease, characterized in that: The following steps are involved: The bacterial suspension or fermentation liquid of Burkholderia gladioli XC-1 is sprayed on the seedlings or plants of Litsea cubeba. The 16SrRNA sequence of Burkholderia gladioli XC-1 is shown in SEQ ID NO:3.

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