140 results about "Genetically modified rice" patented technology

The invention relates to the field of rice gene engineering, and particularly provides OsSULTR2; the invention also discloses application of the 2 protein and the coding gene thereof in regulating and controlling the salt tolerance of rice. The protein meets the following conditions: B1) a protein with an amino acid sequence of SEQ ID NO.1; and B2) a fusion protein with the same function obtained by connecting a tag to the N end and / or C end of B1). The method is used for detecting OsSULTR2; the salt stress phenotype identification in the seedling stage is carried out on the transgenic rice with the gene knockout 1, 2, and the result shows that when the gene segment is deleted, the salt stress tolerance of the rice is improved, and the function and the application way of the gene are proved. Therefore, the OsSULTR2 of the present invention; the 2 protein and the coding gene thereof can regulate and control the salt tolerance of rice, and have important significance for cultivating salt-tolerant transgenic rice.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention relates to the field of rice gene engineering, and discloses an application of OsMYB36 protein and a coding gene thereof in regulation and control of rice salt tolerance. The gene of the OsMYB36 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsMYB36 gene knocked out is remarkably higher than that of a wild-type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsMYB36 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention relates to the technical field of biology, in particular to a rice salt stress regulation gene OsMTA1 and application thereof.The rice salt stress regulation gene OsMTA1 is obtained by conducting CRISPR gene editing on the OsMTA1 gene, and compared with receptor rice Zhonghua 11 (ZH11), after two independent strains with mutants knocked out of the transgenic rice OsMTA1 gene are subjected to 180mM NaCl simulated salt stress treatment, the rice salt stress regulation gene OsMTA1 is obtained. After recovery treatment, phenotype observation and survival rate statistics are carried out, it is confirmed that the osmta1 mutant has a salt-sensitive phenotype compared with wild type ZH11, and it is indicated that the OsMTA1 gene plays an important role in the process of responding to rice salt stress.

The invention relates to the technical field of biology, in particular to application of a rice biological clock gene OsRVED in regulation and control of rice salt stress resistance, and provides application of a rice OsRVED gene as shown in SEQ ID NO.1, a rice OsRVED gene CDS sequence as shown in SEQ ID NO.2 and protein as shown in SEQ ID NO.3 in regulation and control of plant salt stress response. According to the invention, transgenic rice with OsRVED gene mutation is obtained through a CRISPR (clustered regularly interspaced short palindromic repeats) editing technology. After the transgenic rice OsRVED gene knockout mutant is subjected to 180mM NaCl salt stress simulation treatment, the phenotype is observed, the survival rate is counted, it is confirmed that the osrved mutant shows the salt tolerance phenotype compared with receptor rice Zhonghua No.11, and it is indicated that the OsRVED plays an important role in the process of responding to rice salt stress.

The invention relates to the field of plant genetic engineering, and discloses an application of OsAAT2 protein and a coding gene thereof in regulating and controlling the salt tolerance of rice. The gene of the OsAAT2 protein is edited on the basis of a CRISPR / Cas9 technology, a rice ZH11 variety is introduced by utilizing an agrobacterium-mediated method, and a knockout mutant is obtained through screening; tests prove that the rice mutant with the OsAAT2 gene knocked out is rehydrated for 4 days after being subjected to stress treatment of 150 mM NaCl salt, and the survival rate is remarkably higher than that of a wild type ZH11 plant. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsAAT2 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention relates to the field of rice gene engineering, and discloses an application of OsSTA230 protein and a coding gene thereof in regulating and controlling the salt tolerance of rice. The gene of the OsSTA230 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsSTA230 gene knocked out is remarkably higher than that of a wild type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then is rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsSTA230 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention relates to the technical field of gene engineering, in particular to a rice salt stress regulation gene OsBBX11 and application thereof, and the nucleotide sequence of the rice salt stress regulation gene OsBBX11 is as shown in SEQ ID NO. 1. The nucleotide sequence of the CDS sequence of the rice salt stress regulation gene OsBBX11 is as shown in SEQ ID NO. 2. The OsBBX11 gene is subjected to CRISPR (clustered regularly interspaced short palindromic repeats) gene editing to obtain transgenic rice, compared with receptor rice Dongjing rice, after three different strains of the OsBBX11 gene knockout mutant of the transgenic rice are subjected to 180mM NaCl simulated salt stress treatment, the phenotype is observed, the survival rate after recovery treatment is counted, it is confirmed that the osbbx11 mutant shows a salt sensitive phenotype compared with a wild type, and the OsBBX11 gene knockout mutant shows a salt sensitive phenotype. The OsBBX11 gene plays an important role in the process of responding to rice salt stress.

The application discloses application of an OSA3 gene, relates to the technical field of genetic engineering, and the nucleotide sequence of the OSA3 gene is shown as SEQ ID NO. 3. The application research proves that the OSA3 gene has the ability of regulating the deep root ratio of rice, and by using the ability, drought-resistant transgenic rice can be developed for arid regions. Meanwhile, the OSA3 gene knockout mutant osa3-1 / osa3-2 constructed by the application has the increased number of shallow root systems, and can be used for developing transgenic rice resistant to poor soil for poor soil regions, and enhancing the adaptability of rice germplasm to the environment.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention relates to a nucleic acid molecule of a rice transformation event RN85zN-eJ and a detection method. The nucleic acid molecule of the rice RN85zN-eJ comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 1, or a sequence as shown in SEQ ID NO: 2 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 2. The rice transformation event RN85zN-eJ has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic rice event RN85zN-eJ or not.

The present invention relates to the field of bioengineering technology, and in particular to a flanking sequence of the rice transformation event GATV3-2931-2 and a detection method thereof. The right flank sequence of the flanking sequence is shown as SEQ ID NO.1; the left flank sequence of the flanking sequence is shown as SEQ ID NO.2. The present invention provides a set of specific primers for the flanking sequence of the rice transformation event GATV3-2931-2, including nucleotide sequences shown as SEQ ID NO.11-12 and SEQ ID NO.9-10, which can be used to detect whether a biological material contains components derived from the rice transformation event GATV3-2931-2. The specific primer combination provided by the present invention can successfully detect rice transformation events and is of great significance in the biosafety detection and evaluation system of transgenic rice and its derivative lines.

The invention discloses a method for improving the water-resistant straw-like dwarf virus capability of rice based on D14 protein site editing and application of the method, according to the scheme, an interaction interface of RGSV P3 and rice D14 protein is analyzed through a structural biology means, and it is determined that the 102nd part, with the D14 binding site, of D14 and P3 is aspartic acid (Asp, D). Then, accurate editing of the D14 gene in the rice is realized by utilizing a cytidine base editor (CBE) system, and the site is mutated into asparagine (Asn, N), so that D14 (D102N) transgenic rice is obtained. Disease resistance identification confirms that the mutant has significant resistance to RGSV. Furthermore, a homozygous non-transgenic disease-resistant material which does not contain exogenous transgenic ingredients is obtained through genetic screening and has a good breeding application prospect.

The invention belongs to the field of environmental safety evaluation of rice obtained by a biological breeding technology, and discloses a method for determining whether the pollen viability of the rice obtained by the biological breeding technology is different from that of a receptor material of the rice. The method comprises the following steps: culturing transgenic or gene-edited rice pollen and receptor rice pollen in a culture medium, and detecting the difference between the germination rate of the transgenic rice pollen and the germination rate of the receptor rice pollen; the culture medium consists of the following substances: 175 g / L of sucrose, 40 mg / L of boric acid, 20 mg / L of calcium chloride, 10 mg / L of monopotassium phosphate, 10 mg / L of magnesium sulfate and the balance of distilled water. The method has the characteristics of high operability, simple data processing, objective and reliable result and the like. The invention provides a method for measuring pollen viability in environmental safety detection of rice cultivated by using a biological breeding technology, and the method plays an important role in scientific safety evaluation of transgenic and gene-edited rice.

The invention relates to the field of rice gene engineering, and discloses an application of OsSGR1 protein and a coding gene thereof in regulation and control of rice salt tolerance. The gene of the OsSGR1 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsSGR1 gene knocked out is remarkably higher than that of a wild type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then is rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsSGR1 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention discloses a novel coronavirus variant RBD protein gene and application thereof. The method comprises the following steps: firstly, respectively constructing recombinant plant expression vectors pGt1-RBD and pGt1-RBDopt containing RBD and RBDopt genes, carrying out agrobacterium-mediated transformation to obtain a T0-generation transgenic rice plant, and carrying out T0-T3-generation screening and identification to obtain a transgenic rice plant capable of stably expressing RBD protein. The RBD protein is successfully expressed in rice endosperm and is subjected to N-glycosylation modification, and the content of the RBD protein in pGt1: RBDopt is obviously higher than that of pGt1: RBD. The pGt1:: RBDopt transgenic rice flour suspension is orally taken into an intragastric mouse, and experimental results show that the transgenic rice pGt1:: RBDopt can be used for developing oral vaccines of new crown vaccines and has a good application prospect.

The application discloses a gene for regulating brown planthopper resistance of rice METS2 and application thereof, and belongs to the technical field of genetic engineering. METS2 The CDS sequence of the gene is shown as SEQ ID NO. 3. Overexpression of the gene can significantly improve the resistance of rice to the brown planthopper, and provides important gene resources and technical support for rice pest resistance breeding. METS2 The gene overexpression type transgenic rice can be used as a new rice germplasm material, and is used for in-depth research on the brown planthopper resistance mechanism of rice, and for excavation of more genes for regulating the brown planthopper resistance, and has important application value for effectively improving the brown planthopper resistance of rice by means of genetic breeding and genetic engineering methods.

The present application relates to the technical field of genetic engineering, in particular to a rice G1 gene mutant g1-1437, its molecular marker and application. The rice G1 gene mutant g1-1437 provided by the present application takes rice G1 gene as reference sequence, the nucleotide sequence of the rice G1 gene is shown as SEQ ID NO. 8; the gene mutant g1-1437 comprises a mutation site that base C at the 112th position is mutated into base T. The mutation causes the chaff homologous transformation of rice into glume shell substances, which can be used as a phenotype screening marker of transgenic rice. The present application further provides a molecular marker identification method of the mutant, which has a good application prospect in the transference of new germplasm.

The invention provides the following technical scheme: the invention provides application of an LRK6 gene or a recombinant vector containing the LRK6 in improving the heat resistance of rice. The nucleotide sequence of the LRK6 gene is as shown in SEQ ID NO.1, the amino acid sequence of the expression protein of the LRK6 gene is as shown in SEQ ID NO.2, and the recombinant vector containing the LRK6 gene is an expression vector obtained by inserting the LRK6 gene into the expression vector. Experiments show that a transgenic rice strain is obtained by constructing a recombinant vector containing the LRK6 gene and introducing the expression vector into Zhonghua 11 rice by using an agrobacterium-mediated method. Compared with wild-type Zhonghua 11 rice, the transgenic rice strain has higher heat resistance.

The invention discloses an application of a protein OsREM4.1 in increasing the yield of rice. The protein OsREM4.1 is a protein with an amino acid sequence as shown in SEQ ID No.1. The invention also discloses an application of the protein OsREM4.1 in increasing the yield of rice. Experiments prove that transgenic rice with increased yield can be obtained by introducing the gene for coding the protein OsREM4.1 into the rice variety Lehui 188. Therefore, the protein OsREM4.1 can improve the rice yield. The method has an important application value.

The present disclosure provides a construction method of a bacterial cellulose (BC)-enriched plant using multi-gene tandem and use thereof, and relates to the technical field of genetic engineering. In the present disclosure, an acsAB gene, an acsC gene, and an acsD gene are combined and then subjected to codon optimization according to a codon preference of the crop. Resulting optimized genes are fused with a 35S promoter and a nopaline synthase (NOS) terminator to obtain three gene expression cassettes. The three gene expression cassettes are ligated into a plant expression vector to obtain a multi-gene plant transformation vector containing the above three gene expression cassettes. The multi-gene plant transformation vector is then transformed into rice to obtain a transgenic rice plant capable of synthesizing the BC. It is determined that the transgenic rice plant has a BC content of 3.81%.

The invention discloses a gene related to rice tillering as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a gene SSRP1 related to rice tillering is found, and a coding gene of the protein is reversely knocked out through a transgenic technology, so that dwarf multi-tillering transgenic rice is obtained. The tillering of the SSRP1 knockout plant is obviously increased and is 1.8 times of that of wild rice. The results prove that the SSRP1 gene participates in regulating and controlling the tiller number of rice, and can be applied to regulating the plant type and forming a reasonable plant population structure, so that the yield and quality of crops in unit area of cultivated land are improved.

The invention provides an OsSPL17 gene for improving the strength of rice stalks and application of the OsSPL17 gene, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the OsSPL17 gene disclosed by the invention is as shown in SEQ ID NO. 1. According to the invention, a transgenic vector is constructed by cloning a promoter and a genome segment of an OsSPL17 gene, and transgenic rice of which an OsSPL17 self-promoter drives an OsSPL17 self-genome is obtained. Experimental results show that compared with a wild type, the stem thickness, the stem wall thickness and the stem breaking resistance of the transgenic rice are remarkably increased, which indicates that the OsSPL17 plays an important regulation role in rice stem development and can be used for cultivating lodging-resistant rice varieties.

An application of rice NAT protein and a coding gene thereof in regulation and control of plant disease resistance, a method for regulating and controlling rice resistance to rice blast and a method for cultivating transgenic rice with high resistance to rice blast are provided. In the research of rice immune response, a gene NAT of which the translation is induced by M. oryzae is identified and obtained.

A gene related to biosynthesis of ergothioneine is provided. Based on five synthesis genes of ergothioneine in Mycobacteroides abscessus, only an encoding sequence of each of the five genes is retained, and a gene structure of an encoding region in each gene is optimized to obtain genes related to the biosynthesis of the ergothioneine that are stably expressed in rice. These genes have sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 5, and encoded proteins thereof have amino acid sequences set forth in SEQ ID NO: 6 to SEQ ID NO: 10. The gene is transferred into rice to construct a transgenic rice molecular farm to produce the ergothioneine. As a molecular farm, rice seeds do not contain alkaloids or allergens that are harmful to the human body. Moreover, biosynthesized ergothioneine shows the advantages of easy extraction and processing and of low production cost.

The invention discloses application of a sorghum SbPGK1 gene in improving plant disease resistance, and belongs to the technical field of biology. The nucleotide sequence of the sorghum SbPGK1 gene is as shown in SEQ ID NO.9. By analyzing phenotypes and related physiological indexes of SbPGK1 rice transgenic lines before and after inoculation of different pathogenic bacteria for infection and sorghum, the results show that compared with non-SbPGK1 gene rice lines, the SbPGK1 transgenic lines have obviously better resistance to magnaporthe oryzae and bacterial blight germs than wild types, silence SbPGK1 in sorghum has better resistance to rice blast germs and bacterial blight germs, and the SbPGK1 gene has better resistance to rice blast germs and bacterial blight germs. The SbPGK1 gene has the advantages that the SbPGK1 silent strain has reduced disease resistance to colletotrichum gloeosporioides, the SbPGK1 is overexpressed in sorghum, and the SbPGK1 overexpressed strain has enhanced disease resistance to colletotrichum gloeosporioides, so that the SbPGK1 gene is a potential disease-resistant breeding gene and can be used for plant disease-resistant genetic improvement.

The application discloses a method for identifying endochalazal interacting proteins in rice based on a TurboID proximity labeling technology, and belongs to the technical field of biology. The method comprises the following steps: firstly, forming a fusion protein by connecting a target protein with a TurboID biotin ligase, and constructing a transgenic rice plant for stably expressing the fusion protein; secondly, mixing a developing seed of the transgenic plant 7-9 days after flowering with a biotin solution with a concentration of 100-400 muM, performing vacuum treatment for 10-30 min, and then placing the seed for 8-36 h; and thirdly, taking a rice endochalazal sample treated by the biotin to perform protein extraction, enrichment and mass spectrometry identification on the biotin-labeled interacting proteins in the rice endochalazal. The application provides a method for performing proximity space labeling on a target protein in the rice endochalazal based on a TurboID system, and the method can be used for analyzing possible protein-protein interactions.

This invention discloses the application of the SIPP2 gene in improving rice blast resistance, belonging to the field of genetic engineering technology. The nucleotide sequence of the SIPP2 gene is shown in SEQ ID NO.1. This invention discloses the application of the SIPP2 gene in improving rice blast resistance; by knocking out the SIPP2 gene, the resistance of rice to rice blast is effectively improved, and transgenic rice lines with significant resistance to rice blast are obtained.