207 results about "Genetically modified rice" patented technology

The invention relates to the field of rice gene engineering, and particularly provides OsSULTR2; the invention also discloses application of the 2 protein and the coding gene thereof in regulating and controlling the salt tolerance of rice. The protein meets the following conditions: B1) a protein with an amino acid sequence of SEQ ID NO.1; and B2) a fusion protein with the same function obtained by connecting a tag to the N end and / or C end of B1). The method is used for detecting OsSULTR2; the salt stress phenotype identification in the seedling stage is carried out on the transgenic rice with the gene knockout 1, 2, and the result shows that when the gene segment is deleted, the salt stress tolerance of the rice is improved, and the function and the application way of the gene are proved. Therefore, the OsSULTR2 of the present invention; the 2 protein and the coding gene thereof can regulate and control the salt tolerance of rice, and have important significance for cultivating salt-tolerant transgenic rice.

The invention provides a Ustilaginoidea virens gene UvASE2 for regulating and controlling rice resistance and application of the Ustilaginoidea virens gene UvASE2, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 2. By constructing a transgenic rice plant for heterologous expression of UvASE2, it is found that the transgenic rice can significantly improve the resistance to false smut and bacterial blight and significantly induce the outbreak of active oxygen. The heterologous expression UvASE2 gene of Ustilaginoidea virens has the function of positively regulating and controlling the disease resistance of rice, and the UvASE2 gene can be used for improving the disease resistance of rice and has important significance on creation of disease-resistant germplasm of rice.

The invention relates to a nucleic acid molecule of a rice transformation event RN85zN-eH and a detection method. The nucleic acid molecule of the rice RN85zN-eH comprises a sequence shown in SEQ ID NO: 1 or a reverse complementary sequence thereof, or a sequence shown in SEQ ID NO: 2 or a reverse complementary sequence thereof. The rice transformation event RN85zN-eH disclosed by the invention has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic rice event RN85zN-eH or not.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention discloses a rice OsFb34 gene as well as an encoding protein and application thereof, and relates to the technical field of plant genetic engineering. The CDS nucleotide sequence of the rice OsFb34 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the rice OsFb34 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing a knockout vector of a rice OsFb34 gene; transforming the constructed knockout vector into rice; and cultivating and screening to obtain a transgenic rice plant with reduced rice grain length, increased grain width and reduced length / width ratio. The mutants of osfb34-1 and osfb34-2 both show phenotypes that the grain length is reduced and the grain width is increased, so that the ratio of the length / width ratio is reduced. The result shows that the OsFb34 gene deletion can cause the reduction of the grain length and the increase of the grain width of the rice, and further cause the reduction of the length / width ratio.

The invention relates to the field of rice gene engineering, and discloses an application of OsMYB36 protein and a coding gene thereof in regulation and control of rice salt tolerance. The gene of the OsMYB36 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsMYB36 gene knocked out is remarkably higher than that of a wild-type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsMYB36 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention provides an arsenic hyperaccumulation double-gene recombinant vector and application of the arsenic hyperaccumulation double-gene recombinant vector in repairing an arsenic polluted environment, and belongs to the technical field of gene engineering. The arsenic hyperaccumulation double-gene recombinant vector takes a plant expression vector containing a pUbi promoter as a skeleton vector, and further comprises a PvACR3 gene and PvACR3, 1 gene and a prbcS promoter; wherein the pUbi promoter is used for promoting the expression of the PvACR3 gene; a prbcS promoter is used for starting the PvACR3; 1 gene expression. Aiming at the problem of rice death caused by high expression quantity of arsenic accumulation genes in transgenic rice in the prior art, through reasonable design of insertion genes and combination of promoters, toxicity of arsenic accumulation to the transgenic rice is avoided, and the arsenic removal quantity of the transgenic rice is greatly improved.

The invention belongs to the technical field of herbicide resistance genes, and relates to a rice herbicide resistance gene and application thereof. The herbicide-resistant gene provided by the invention is OsCYP704A5, and the sequence of the herbicide-resistant gene is as shown in SEQ ID NO. 1. According to the application disclosed by the invention, molecular docking analysis is carried out on the OsCYP704A5 gene and the triazole sulcotrione, and the result shows that the OsCYP704A5 and the triazole sulcotrione have relatively high combining capacity and have a certain promoting effect on detoxification metabolism. The OsCYP704A5 gene is further over-expressed in Nipponbare rice, so that the transgenic rice can obtain the drug resistance to triazole sulcotrione. The HPPD inhibitor herbicide-resistant rice strain can be used for cultivating HPPD inhibitor herbicide-resistant rice varieties, and provides a new possibility for researching HPPD inhibitor herbicide resistance of paddy field weeds (barnyard grass).

The invention relates to the technical field of biology, in particular to a rice salt stress regulation gene OsMTA1 and application thereof.The rice salt stress regulation gene OsMTA1 is obtained by conducting CRISPR gene editing on the OsMTA1 gene, and compared with receptor rice Zhonghua 11 (ZH11), after two independent strains with mutants knocked out of the transgenic rice OsMTA1 gene are subjected to 180mM NaCl simulated salt stress treatment, the rice salt stress regulation gene OsMTA1 is obtained. After recovery treatment, phenotype observation and survival rate statistics are carried out, it is confirmed that the osmta1 mutant has a salt-sensitive phenotype compared with wild type ZH11, and it is indicated that the OsMTA1 gene plays an important role in the process of responding to rice salt stress.

The invention relates to the technical field of biology, in particular to application of a rice biological clock gene OsRVED in regulation and control of rice salt stress resistance, and provides application of a rice OsRVED gene as shown in SEQ ID NO.1, a rice OsRVED gene CDS sequence as shown in SEQ ID NO.2 and protein as shown in SEQ ID NO.3 in regulation and control of plant salt stress response. According to the invention, transgenic rice with OsRVED gene mutation is obtained through a CRISPR (clustered regularly interspaced short palindromic repeats) editing technology. After the transgenic rice OsRVED gene knockout mutant is subjected to 180mM NaCl salt stress simulation treatment, the phenotype is observed, the survival rate is counted, it is confirmed that the osrved mutant shows the salt tolerance phenotype compared with receptor rice Zhonghua No.11, and it is indicated that the OsRVED plays an important role in the process of responding to rice salt stress.

The invention discloses rice LOC_Os01g68930 The application of genes in regulating the protein content in rice grains relates to the field of genetic engineering technology. LOC_Os01g68930 The CDS sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence of the protein encoded by it is shown in SEQ ID NO. 2. The present invention obtains OE‑LOC_Os01g68930 Overexpression plants. Experiments have shown that: PLACE_ Os01g68930 The total content of glutenin and alcohol-soluble proteins in the seeds of overexpressing transgenic rice reached 7.88% to 7.97%. In comparison, the protein content of wild-type rice grains was 6.97%, and the protein content of transgenic rice grains increased by 13.1%-14.3%. LOC_Os01g68930 It has been confirmed that this gene can effectively regulate the protein content of rice grains, laying a solid foundation for the subsequent breeding of high-protein rice varieties.

As a main grain crop in the world, the yield and quality of rice are often threatened by bacterial leaf blight and rice blast. According to the invention, a recombinant expression vector for expressing STTM-miR169d / p is constructed by starting from regulation and control of the expression quantity of miRNA through an STTM technology. By adopting an agrobacterium tumefaciens-mediated rice protoplast transformation technology, a rice plant containing the STTM-miR169d / p transgenic fragment is obtained. Through field inoculation of xanthomonas oryzae pv. Oryzae and inoculation of rice blast spores on in-vitro leaves, it is verified that the STTM-miR169d / p transgenic rice has significantly improved resistance to xanthomonas oryzae pv. Oryzae and rice blast compared with wild type rice. The invention not only provides a new germplasm resource for cultivating disease-resistant rice, but also provides a new thought for regulating and controlling the disease resistance of rice from the aspect of miRNA.

The invention relates to the field of plant genetic engineering, and discloses an application of OsAAT2 protein and a coding gene thereof in regulating and controlling the salt tolerance of rice. The gene of the OsAAT2 protein is edited on the basis of a CRISPR / Cas9 technology, a rice ZH11 variety is introduced by utilizing an agrobacterium-mediated method, and a knockout mutant is obtained through screening; tests prove that the rice mutant with the OsAAT2 gene knocked out is rehydrated for 4 days after being subjected to stress treatment of 150 mM NaCl salt, and the survival rate is remarkably higher than that of a wild type ZH11 plant. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsAAT2 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

A gene related to biosynthesis of ergothioneine is provided. Based on five synthesis genes of ergothioneine in Mycobacteroides abscessus, only an encoding sequence of each of the five genes is retained, and a gene structure of an encoding region in each gene is optimized to obtain genes related to the biosynthesis of the ergothioneine that are stably expressed in rice. These genes have sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 5, and encoded proteins thereof have amino acid sequences set forth in SEQ ID NO: 6 to SEQ ID NO: 10. The gene is transferred into rice to construct a transgenic rice molecular farm to produce the ergothioneine. As a molecular farm, rice seeds do not contain alkaloids or allergens that are harmful to the human body. Moreover, biosynthesized ergothioneine shows the advantages of easy extraction and processing and of low production cost.

The invention discloses a saline-alkaline tolerance gene introduced rice high-yield breeding method. The method comprises the following steps: S1, gene screening and cloning: screening related genes with saline-alkaline tolerance; s2, vector construction: inserting the screened saline-alkaline tolerant gene into a proper expression vector; s3, transforming rice cells: introducing the vector carrying the saline-alkaline tolerant gene into the rice cells; s4, screening positive plants: confirming that the rice cells with the saline-alkaline tolerant genes are successfully transferred, and selecting the positive plants from the rice cells for next-step culture; s5, tissue culture and development: placing the positive plants on a sterile culture medium for tissue culture, and enabling the positive plants to develop into complete plants; s6, planting and harvesting: transplanting and planting the obtained transgenic rice plants, and harvesting the transgenic rice plants to obtain rice seeds. The saline-alkali soil-resistant gene disclosed by the invention is stronger in saline-alkali resistance, the yield is not influenced after the gene is planted in saline-alkali soil, and high-yield rice can be obtained.

The invention relates to the field of rice gene engineering, and discloses an application of OsSTA230 protein and a coding gene thereof in regulating and controlling the salt tolerance of rice. The gene of the OsSTA230 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsSTA230 gene knocked out is remarkably higher than that of a wild type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then is rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsSTA230 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention relates to the technical field of gene engineering, in particular to a rice salt stress regulation gene OsBBX11 and application thereof, and the nucleotide sequence of the rice salt stress regulation gene OsBBX11 is as shown in SEQ ID NO. 1. The nucleotide sequence of the CDS sequence of the rice salt stress regulation gene OsBBX11 is as shown in SEQ ID NO. 2. The OsBBX11 gene is subjected to CRISPR (clustered regularly interspaced short palindromic repeats) gene editing to obtain transgenic rice, compared with receptor rice Dongjing rice, after three different strains of the OsBBX11 gene knockout mutant of the transgenic rice are subjected to 180mM NaCl simulated salt stress treatment, the phenotype is observed, the survival rate after recovery treatment is counted, it is confirmed that the osbbx11 mutant shows a salt sensitive phenotype compared with a wild type, and the OsBBX11 gene knockout mutant shows a salt sensitive phenotype. The OsBBX11 gene plays an important role in the process of responding to rice salt stress.

The invention relates to a nucleic acid molecule of a rice transformation event RN85N-e3 and a detection method. The nucleic acid molecule of the rice transformation event RN85N-e3 comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 1, or a sequence as shown in SEQ ID NO: 2 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 2. The rice transformation event RN85N-e3 disclosed by the invention has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic rice event RN85N-e3 or not.

The application discloses application of an OSA3 gene, relates to the technical field of genetic engineering, and the nucleotide sequence of the OSA3 gene is shown as SEQ ID NO. 3. The application research proves that the OSA3 gene has the ability of regulating the deep root ratio of rice, and by using the ability, drought-resistant transgenic rice can be developed for arid regions. Meanwhile, the OSA3 gene knockout mutant osa3-1 / osa3-2 constructed by the application has the increased number of shallow root systems, and can be used for developing transgenic rice resistant to poor soil for poor soil regions, and enhancing the adaptability of rice germplasm to the environment.

The invention provides application of a ring finger protein coding gene OsRF in regulation and control of rice grain shape and grain weight, the gene OsRF for coding the ring finger protein is separated and cloned from rice Nipponbare, and the gene is targeted by rice small RNA OsmiR530. In order to prevent the OsmiR530 from being cut and improve the expression level of the OsmiR530, artificial synonymous mutation is carried out on the OsRF recognized sequence by the OsmiR530, a mutant OsRF fragment (mOsRF) of which the recognition sequence is subjected to synonymous mutation is created by utilizing overlapping PCR (Polymerase Chain Reaction), an overexpression vector of the mOsRF is constructed and is overexpressed in rice, and the grain width and the grain weight of transgenic rice are obviously increased. Therefore, the overexpression of the mOsRF in the rice is of great significance for obtaining round-grain rice and increasing the rice yield, and a new resource and a new research method are provided for rice grain shape improvement breeding.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention relates to a nucleic acid molecule of a rice transformation event RN85zN-eJ and a detection method. The nucleic acid molecule of the rice RN85zN-eJ comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 1, or a sequence as shown in SEQ ID NO: 2 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 2. The rice transformation event RN85zN-eJ has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic rice event RN85zN-eJ or not.

The present invention relates to the field of bioengineering technology, and in particular to a flanking sequence of the rice transformation event GATV3-2931-2 and a detection method thereof. The right flank sequence of the flanking sequence is shown as SEQ ID NO.1; the left flank sequence of the flanking sequence is shown as SEQ ID NO.2. The present invention provides a set of specific primers for the flanking sequence of the rice transformation event GATV3-2931-2, including nucleotide sequences shown as SEQ ID NO.11-12 and SEQ ID NO.9-10, which can be used to detect whether a biological material contains components derived from the rice transformation event GATV3-2931-2. The specific primer combination provided by the present invention can successfully detect rice transformation events and is of great significance in the biosafety detection and evaluation system of transgenic rice and its derivative lines.

The invention discloses a method for improving the water-resistant straw-like dwarf virus capability of rice based on D14 protein site editing and application of the method, according to the scheme, an interaction interface of RGSV P3 and rice D14 protein is analyzed through a structural biology means, and it is determined that the 102nd part, with the D14 binding site, of D14 and P3 is aspartic acid (Asp, D). Then, accurate editing of the D14 gene in the rice is realized by utilizing a cytidine base editor (CBE) system, and the site is mutated into asparagine (Asn, N), so that D14 (D102N) transgenic rice is obtained. Disease resistance identification confirms that the mutant has significant resistance to RGSV. Furthermore, a homozygous non-transgenic disease-resistant material which does not contain exogenous transgenic ingredients is obtained through genetic screening and has a good breeding application prospect.

The invention belongs to the field of environmental safety evaluation of rice obtained by a biological breeding technology, and discloses a method for determining whether the pollen viability of the rice obtained by the biological breeding technology is different from that of a receptor material of the rice. The method comprises the following steps: culturing transgenic or gene-edited rice pollen and receptor rice pollen in a culture medium, and detecting the difference between the germination rate of the transgenic rice pollen and the germination rate of the receptor rice pollen; the culture medium consists of the following substances: 175 g / L of sucrose, 40 mg / L of boric acid, 20 mg / L of calcium chloride, 10 mg / L of monopotassium phosphate, 10 mg / L of magnesium sulfate and the balance of distilled water. The method has the characteristics of high operability, simple data processing, objective and reliable result and the like. The invention provides a method for measuring pollen viability in environmental safety detection of rice cultivated by using a biological breeding technology, and the method plays an important role in scientific safety evaluation of transgenic and gene-edited rice.

The invention relates to the field of rice gene engineering, and discloses an application of OsSGR1 protein and a coding gene thereof in regulation and control of rice salt tolerance. The gene of the OsSGR1 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsSGR1 gene knocked out is remarkably higher than that of a wild type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then is rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsSGR1 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention discloses a novel coronavirus variant RBD protein gene and application thereof. The method comprises the following steps: firstly, respectively constructing recombinant plant expression vectors pGt1-RBD and pGt1-RBDopt containing RBD and RBDopt genes, carrying out agrobacterium-mediated transformation to obtain a T0-generation transgenic rice plant, and carrying out T0-T3-generation screening and identification to obtain a transgenic rice plant capable of stably expressing RBD protein. The RBD protein is successfully expressed in rice endosperm and is subjected to N-glycosylation modification, and the content of the RBD protein in pGt1: RBDopt is obviously higher than that of pGt1: RBD. The pGt1:: RBDopt transgenic rice flour suspension is orally taken into an intragastric mouse, and experimental results show that the transgenic rice pGt1:: RBDopt can be used for developing oral vaccines of new crown vaccines and has a good application prospect.

The application discloses a gene for regulating brown planthopper resistance of rice METS2 and application thereof, and belongs to the technical field of genetic engineering. METS2 The CDS sequence of the gene is shown as SEQ ID NO. 3. Overexpression of the gene can significantly improve the resistance of rice to the brown planthopper, and provides important gene resources and technical support for rice pest resistance breeding. METS2 The gene overexpression type transgenic rice can be used as a new rice germplasm material, and is used for in-depth research on the brown planthopper resistance mechanism of rice, and for excavation of more genes for regulating the brown planthopper resistance, and has important application value for effectively improving the brown planthopper resistance of rice by means of genetic breeding and genetic engineering methods.

The present application relates to the technical field of genetic engineering, in particular to a rice G1 gene mutant g1-1437, its molecular marker and application. The rice G1 gene mutant g1-1437 provided by the present application takes rice G1 gene as reference sequence, the nucleotide sequence of the rice G1 gene is shown as SEQ ID NO. 8; the gene mutant g1-1437 comprises a mutation site that base C at the 112th position is mutated into base T. The mutation causes the chaff homologous transformation of rice into glume shell substances, which can be used as a phenotype screening marker of transgenic rice. The present application further provides a molecular marker identification method of the mutant, which has a good application prospect in the transference of new germplasm.