94 results about "Genetically modified rice" patented technology

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention relates to the field of rice gene engineering, and discloses an application of OsMYB36 protein and a coding gene thereof in regulation and control of rice salt tolerance. The gene of the OsMYB36 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsMYB36 gene knocked out is remarkably higher than that of a wild-type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsMYB36 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The invention relates to the field of plant genetic engineering, and discloses an application of OsAAT2 protein and a coding gene thereof in regulating and controlling the salt tolerance of rice. The gene of the OsAAT2 protein is edited on the basis of a CRISPR / Cas9 technology, a rice ZH11 variety is introduced by utilizing an agrobacterium-mediated method, and a knockout mutant is obtained through screening; tests prove that the rice mutant with the OsAAT2 gene knocked out is rehydrated for 4 days after being subjected to stress treatment of 150 mM NaCl salt, and the survival rate is remarkably higher than that of a wild type ZH11 plant. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsAAT2 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The application discloses application of an OSA3 gene, relates to the technical field of genetic engineering, and the nucleotide sequence of the OSA3 gene is shown as SEQ ID NO. 3. The application research proves that the OSA3 gene has the ability of regulating the deep root ratio of rice, and by using the ability, drought-resistant transgenic rice can be developed for arid regions. Meanwhile, the OSA3 gene knockout mutant osa3-1 / osa3-2 constructed by the application has the increased number of shallow root systems, and can be used for developing transgenic rice resistant to poor soil for poor soil regions, and enhancing the adaptability of rice germplasm to the environment.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention discloses a method for improving the water-resistant straw-like dwarf virus capability of rice based on D14 protein site editing and application of the method, according to the scheme, an interaction interface of RGSV P3 and rice D14 protein is analyzed through a structural biology means, and it is determined that the 102nd part, with the D14 binding site, of D14 and P3 is aspartic acid (Asp, D). Then, accurate editing of the D14 gene in the rice is realized by utilizing a cytidine base editor (CBE) system, and the site is mutated into asparagine (Asn, N), so that D14 (D102N) transgenic rice is obtained. Disease resistance identification confirms that the mutant has significant resistance to RGSV. Furthermore, a homozygous non-transgenic disease-resistant material which does not contain exogenous transgenic ingredients is obtained through genetic screening and has a good breeding application prospect.

The invention relates to the field of rice gene engineering, and discloses an application of OsSGR1 protein and a coding gene thereof in regulation and control of rice salt tolerance. The gene of the OsSGR1 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsSGR1 gene knocked out is remarkably higher than that of a wild type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then is rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsSGR1 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.

The application discloses a gene for regulating brown planthopper resistance of rice METS2 and application thereof, and belongs to the technical field of genetic engineering. METS2 The CDS sequence of the gene is shown as SEQ ID NO. 3. Overexpression of the gene can significantly improve the resistance of rice to the brown planthopper, and provides important gene resources and technical support for rice pest resistance breeding. METS2 The gene overexpression type transgenic rice can be used as a new rice germplasm material, and is used for in-depth research on the brown planthopper resistance mechanism of rice, and for excavation of more genes for regulating the brown planthopper resistance, and has important application value for effectively improving the brown planthopper resistance of rice by means of genetic breeding and genetic engineering methods.

The present application relates to the technical field of genetic engineering, in particular to a rice G1 gene mutant g1-1437, its molecular marker and application. The rice G1 gene mutant g1-1437 provided by the present application takes rice G1 gene as reference sequence, the nucleotide sequence of the rice G1 gene is shown as SEQ ID NO. 8; the gene mutant g1-1437 comprises a mutation site that base C at the 112th position is mutated into base T. The mutation causes the chaff homologous transformation of rice into glume shell substances, which can be used as a phenotype screening marker of transgenic rice. The present application further provides a molecular marker identification method of the mutant, which has a good application prospect in the transference of new germplasm.

The invention provides the following technical scheme: the invention provides application of an LRK6 gene or a recombinant vector containing the LRK6 in improving the heat resistance of rice. The nucleotide sequence of the LRK6 gene is as shown in SEQ ID NO.1, the amino acid sequence of the expression protein of the LRK6 gene is as shown in SEQ ID NO.2, and the recombinant vector containing the LRK6 gene is an expression vector obtained by inserting the LRK6 gene into the expression vector. Experiments show that a transgenic rice strain is obtained by constructing a recombinant vector containing the LRK6 gene and introducing the expression vector into Zhonghua 11 rice by using an agrobacterium-mediated method. Compared with wild-type Zhonghua 11 rice, the transgenic rice strain has higher heat resistance.

The invention discloses an application of a protein OsREM4.1 in increasing the yield of rice. The protein OsREM4.1 is a protein with an amino acid sequence as shown in SEQ ID No.1. The invention also discloses an application of the protein OsREM4.1 in increasing the yield of rice. Experiments prove that transgenic rice with increased yield can be obtained by introducing the gene for coding the protein OsREM4.1 into the rice variety Lehui 188. Therefore, the protein OsREM4.1 can improve the rice yield. The method has an important application value.

The invention discloses a gene related to rice tillering as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a gene SSRP1 related to rice tillering is found, and a coding gene of the protein is reversely knocked out through a transgenic technology, so that dwarf multi-tillering transgenic rice is obtained. The tillering of the SSRP1 knockout plant is obviously increased and is 1.8 times of that of wild rice. The results prove that the SSRP1 gene participates in regulating and controlling the tiller number of rice, and can be applied to regulating the plant type and forming a reasonable plant population structure, so that the yield and quality of crops in unit area of cultivated land are improved.

The invention provides an OsSPL17 gene for improving the strength of rice stalks and application of the OsSPL17 gene, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the OsSPL17 gene disclosed by the invention is as shown in SEQ ID NO. 1. According to the invention, a transgenic vector is constructed by cloning a promoter and a genome segment of an OsSPL17 gene, and transgenic rice of which an OsSPL17 self-promoter drives an OsSPL17 self-genome is obtained. Experimental results show that compared with a wild type, the stem thickness, the stem wall thickness and the stem breaking resistance of the transgenic rice are remarkably increased, which indicates that the OsSPL17 plays an important regulation role in rice stem development and can be used for cultivating lodging-resistant rice varieties.

An application of rice NAT protein and a coding gene thereof in regulation and control of plant disease resistance, a method for regulating and controlling rice resistance to rice blast and a method for cultivating transgenic rice with high resistance to rice blast are provided. In the research of rice immune response, a gene NAT of which the translation is induced by M. oryzae is identified and obtained.

A gene related to biosynthesis of ergothioneine is provided. Based on five synthesis genes of ergothioneine in Mycobacteroides abscessus, only an encoding sequence of each of the five genes is retained, and a gene structure of an encoding region in each gene is optimized to obtain genes related to the biosynthesis of the ergothioneine that are stably expressed in rice. These genes have sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 5, and encoded proteins thereof have amino acid sequences set forth in SEQ ID NO: 6 to SEQ ID NO: 10. The gene is transferred into rice to construct a transgenic rice molecular farm to produce the ergothioneine. As a molecular farm, rice seeds do not contain alkaloids or allergens that are harmful to the human body. Moreover, biosynthesized ergothioneine shows the advantages of easy extraction and processing and of low production cost.

The application discloses a method for identifying endochalazal interacting proteins in rice based on a TurboID proximity labeling technology, and belongs to the technical field of biology. The method comprises the following steps: firstly, forming a fusion protein by connecting a target protein with a TurboID biotin ligase, and constructing a transgenic rice plant for stably expressing the fusion protein; secondly, mixing a developing seed of the transgenic plant 7-9 days after flowering with a biotin solution with a concentration of 100-400 muM, performing vacuum treatment for 10-30 min, and then placing the seed for 8-36 h; and thirdly, taking a rice endochalazal sample treated by the biotin to perform protein extraction, enrichment and mass spectrometry identification on the biotin-labeled interacting proteins in the rice endochalazal. The application provides a method for performing proximity space labeling on a target protein in the rice endochalazal based on a TurboID system, and the method can be used for analyzing possible protein-protein interactions.

This invention discloses the application of the SIPP2 gene in improving rice blast resistance, belonging to the field of genetic engineering technology. The nucleotide sequence of the SIPP2 gene is shown in SEQ ID NO.1. This invention discloses the application of the SIPP2 gene in improving rice blast resistance; by knocking out the SIPP2 gene, the resistance of rice to rice blast is effectively improved, and transgenic rice lines with significant resistance to rice blast are obtained.

The present disclosure relates to a method for producing plants with an enhanced carotenoid content using CRISPR / Cas9-mediated gene editing. It was confirmed that transformed rice was produced using the CRISPR / Cas9 system, in which rice-derived Oryza sativa carotenoid cleavage dioxygenase 1 (OsCCD1), Oryza sativa carotenoid cleavage dioxygenase 4a (OsCCD4a), and Oryza sativa carotenoid cleavage dioxygenase 4b (OsCCD4b) genes were deleted, and the carotenoid content was enhanced in the produced transformed rice. Therefore, the present disclosure can be used for producing plants with an enhanced carotenoid content.

The invention discloses an application of RWR5 protein and a coding gene thereof in improving disease resistance of rice. According to the invention, an RWR5 gene is constructed on a vector pCAMBIA1300-221-flag to form a recombinant vector pCAMBIA1300:: RWR5, and then the recombinant vector pCAMBIA1300:: RWR5 is transformed into a rice variety TP309 by an agrobacterium-mediated genetic transformation method, so that RWR5 transgenic rice strains NLR # 1 and NLR # 2 are obtained. Transgenic tests find that overexpression of the RWR5 can enhance the rice blast resistance of rice, which is of great significance to improvement of plant disease resistance and cultivation of disease-resistant plant varieties.

The invention relates to the technical field of biology, in particular to application of an OsJAZ12 gene in regulation and control of plant salt stress response. According to the invention, the OsJAZ12 gene is subjected to CRISPR gene editing to obtain transgenic rice. Compared with a receptor rice ZH11, after two different strains of the transgenic rice OsJAZ12 gene knockout mutant are subjected to 180mM NaCl simulated salt stress treatment, the phenotype is observed, the survival rate after recovery treatment is counted, it is confirmed that the OsJAZ12 mutant shows a salt-sensitive phenotype compared with a wild type, and the OsJAZ12 plays an important role in the process of responding to rice salt stress.

The invention provides application of an OsCML36 gene in improving resistance of rice to sheath blight disease, and belongs to the technical field of gene engineering. The overexpression rice OsCML36 gene is applied to any one of the following applications: (1) the resistance of rice to sheath blight is improved; (2) the agronomic traits of the rice are changed; (3) cultivating transgenic rice with strong sheath blight resistance; (4) cultivating transgenic rice with changed agronomic characters; the nucleotide sequence of the rice OsCML36 gene is as shown in SEQ ID NO. 1. The application of the OsCML36 gene in regulating and controlling the resistance and agronomic traits of the rice sheath blight disease has important significance in analyzing the disease-resistant mechanism of the gene and cultivating rice plants with high disease resistance and high quality.

The present application relates to the technical field of biology, and particularly relates to a salt stress regulation gene OsMTA1 of rice and application thereof, the present application obtains the transgenic rice after CRISPR gene editing of the OsMTA1 gene, compared with the receptor rice Zhonghua 11 (ZH11), after 180mM NaCl simulation salt stress treatment of two independent strains of the transgenic rice OsMTA1 gene knockout mutant, the phenotype is observed after recovery treatment and the survival rate is counted, it is confirmed that the osmta1 mutant presents salt-sensitive phenotype compared with the wild type ZH11, which indicates that the OsMTA1 gene plays an important role in the process of responding to salt stress of rice.

The invention discloses an application of an OsHDA701 gene in regulation and control of drought resistance of rice. According to the application of the OsHDA701 gene in regulating and controlling the drought resistance of the rice, the nucleotide sequence of the OsHDA701 gene is as shown in SEQ ID NO. 1. The rice drought resistance is specifically and negatively regulated by the OsHDA701 gene, and the rice drought resistance can be enhanced by knocking out the OsHDA701 gene or inhibiting the expression of the OsHDA701 gene. According to the application, it is clear that the OsHDA701 gene serves as a negative regulatory factor to control the drought resistance of the rice for the first time, and a new molecular target is provided for genetic improvement of the rice. By inhibiting the function of the negative regulation gene OsHDA701, the inherent stress resistance potential of the rice can be released, so that the drought resistance of the transgenic rice is enhanced, and the survival rate of the transgenic rice under drought stress is obviously higher than that of wild rice. According to the method disclosed by the invention, an OsHDA701 gene mutant plant is created through a mature gene editing technology, and the breeding process is accurate and rapid.

This invention provides the TaGlo1 protein and a recombinant plasmid for regulating arsenic and cadmium tolerance in rice. The recombinant plasmid includes an RCc3 promoter and a gene encoding the TaGlo1 protein. This invention also provides a transgenic rice variety with arsenic and cadmium tolerance, which expresses the TaGlo1 protein. This invention verifies that TaGlo1 gene expression significantly enhances rice resistance to arsenic and cadmium stress and promotes plant growth. Furthermore, the transgenic rice of this invention exhibits enhanced root accumulation of arsenic and cadmium, but the arsenic and cadmium content in the stem is not significantly different from the wild type. Combined with root-stem translocation coefficient analysis, it is confirmed that TaGlo1 expression can reduce the translocation efficiency of arsenic and cadmium from the roots to the aboveground parts, thus effectively controlling the migration of arsenic and cadmium to edible parts.

The invention discloses a rice ferritin mutant with improved thermal stability, a coding gene and application of the rice ferritin mutant, the mutant is rice ferritin, an extended peptide EP segment amino acid sequence of the mutant is replaced by an EP segment amino acid sequence of soybean ferritin, and the thermal stability of the rice ferritin is improved; after the gene encoding the mutant is introduced into rice, the head rice rate of transgenic rice grains is increased, the chalkiness degree is reduced, the total starch content and amylose content are increased, the total protein content and the soluble sugar content are reduced, the iron content of the transgenic rice grains is remarkably increased, the iron content of cooked rice is increased by 1.57-1.95 times compared with that of wild rice, and the rice quality is improved. The problem of serious iron loss of natural ferritin in the cooking process is partially solved. The thermal stability of the obtained rice ferritin is remarkably improved, and the rice ferritin can be directly used for producing high-quality polished rice, can also be used as a raw material for preparing functional foods such as high-iron high-amylose rice flour and low-glycemic-index rice products, and has important industrialization value and wide market application prospects.

The application discloses a breeding method for synthesizing resveratrol and pterostilbene in rice seed endosperm, and relates to the technical field of biology.The transgenic breeding method comprises the steps of transforming a recombinant carrier into rice callus and then cultivating the rice callus into a transgenic rice plant; the recombinant carrier comprises a gene expression cassette for expressing VvSTS protein, a gene expression cassette for expressing VvROMT protein, a gene expression cassette for expressing At4CL protein and a gene expression cassette for expressing transcription factor OsMYBP1 protein.The application realizes the synthesis of resveratrol and pterostilbene in rice endosperm, and the product can be used as raw material for producing resveratrol and pterostilbene, and has important guiding significance for new functional breeding.

This invention belongs to the field of plant genetic engineering technology, specifically relating to a method for improving the disease resistance of rice. SCRE10 Genes and their applications, the aforementioned SCRE10 The base sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention utilizes a dexamethasone-induced expression mechanism. SCRE10 The transgenic rice plants were found to significantly induce... PR Gene expression and reactive oxygen species surges, enhancing rice resistance to rice false smut and bacterial blight. This invention demonstrates heterologous induction of rice false smut pathogen expression. SCRE10 Genes have the function of positively regulating the disease resistance of rice. SCRE10 Genes can be used to improve the disease resistance of rice, which is of great significance for the creation of disease-resistant rice germplasm.

The application discloses a bamboo plasma membrane H + ATPase, its gene PePMHA9 and application, belongs to the technical field of genetic engineering, and particularly relates to a bamboo plasma membrane H + ATPase, a bamboo plasma membrane H + The amino acid sequence of the bamboo plasma membrane H ATPase is shown as SEQ ID NO. 1. A coding gene PePMHA9 of the bamboo plasma membrane H ATPase, and the nucleotide sequence of the coding gene PePMHA9 is shown as SEQ ID NO. 2. The bamboo plasma membrane H ATPase or the coding gene PePMHA9 is applied to promoting the growth of rice in a high light environment. The bamboo plasma membrane H + ATPase and its coding gene PePMHA9 are successfully separated and identified from bamboo, and are transferred into wild type rice through a transgenic technology to perform function verification, and the obtained transgenic rice has increased leaf stomatal aperture, improved photosynthetic capacity and increased biomass in the high light environment compared with the wild type rice.

The invention discloses a breeding method for synthesizing resveratrol and pterostilbene in rice seed endosperm, and relates to the technical field of biology. The transgenic breeding method comprises the following steps: transforming a recombinant vector into a rice callus, and culturing into a transgenic rice plant, the recombinant vector comprises a gene expression cassette for expressing VvSTS protein, a gene expression cassette for expressing VvROMT protein, a gene expression cassette for expressing At4CL protein and a gene expression cassette for expressing transcription factor OsMYBP1 protein. The synthesis of the resveratrol and the pterostilbene in the rice endosperm is realized, and the product can be used as a raw material for producing the resveratrol and the pterostilbene, and has important guiding significance for novel functional breeding.