98 results about "Storage protein" patented technology

The present invention relates to thermostable proteases having an amino acid sequence which homologous to the amino acid sequence of proteases derived from Nocardiopsis, and the production thereof by wild-type and recombinant host cells including transgenic plants and non-human transgenic animals. The proteases are effective in animal feed, in particular fish feed, and detergents. The proteases are capable of degrading the soybean Bowman-Birk inhibitor, and other antinutritional factors such as soybean agglutinin and the Kunitz trypsin inhibitor, as well as the isolated soy storage proteins glycinin and beta-conglycinin. Characteristic structural features of relevance for the thermostability of these proteases of peptidase family S2A or S1E are disclosed.

It is intended to provide a method of reducing the total amount of stored protein, to develop a technique required therefor, and to provide a plant and its seeds developed by the above method and a method of using such a plant and seeds. More specifically, a nucleic acid molecule containing a consecutive nucleic acid sequence of at least 15 in length which is complementary with a nucleic acid sequence encoding prolamine polypeptide or a nucleic acid sequence having a homology of at least about 70% to the complementary nucleic acid sequence having at least 15 nucleotide length. A method of reducing the expression dose of a protein in a seed of a plant which comprises: A) the step of providing the above-described nucleic acid molecule; B) the step of transferring the nucleic acid molecule into the cells of the plant; C) the step of re-differentiating the cells to construct a transgenic plant; and D) the step of obtaining seeds from the transgenic plant.

A method of producing human serum albumin (HSA) in monocot plant seeds, by transforming a monocot plant cell with a chimeric gene containing a promoter from the gene of a maturation-specific monocot plant storage protein, a first DNA sequence operably linked to the promoter and encoding a signal sequence, and a second DNA sequence linked in translation frame with the first DNA sequence and encoding HSA, where the first DNA sequence and the second DNA sequence together encode a fusion protein containing an N-terminal signal sequence and the HSA; growing a monocot plant from the transformed monocot plant cell for a time sufficient to produce seeds containing the HSA; and harvesting the seeds from the plant.

The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

The expression of heterologous peptides or polypeptides in the seeds of monocot plants is optimized by generating fusion protein constructs in which monocot plant seed storage proteins are used as fusion protein carriers for the heterologous peptides or polypeptides. The heterologous peptides or polypeptides are preferably small, about 10 kDa or less and/or between 5 and 100 amino acids in length. These heterologous peptides or polypeptides may be used in human and animal nutritional and therapeutic compositions.

The invention is directed to expression of non-plant proteins in rice plants. Expression is optimized by use of a non-rice promoter of a monocot protein gene and its corresponding signal peptide for expression of the non-plant protein in rice plant at high yields. The invention is useful for making human proteins, polypeptides and peptides in rice seeds. The expressed protein product can be isolated from the rice seed for administration to humans or other animals.

A transformed soybean plant having a gene encoding a modified seed storage protein introduced therein, obtained by inserting a gene encoding an Alzheimer's disease vaccine to a variable region(s) of a gene encoding a wild-type seed storage protein, is produced, and said vaccine is produced and accumulated in the seeds thereof.

Gluten-free baked products, particularly gluten-free bread products, which exhibit properties comparable those made with wheat flour. Compositions and methods for preparation of gluten-free baked products. A conditioned protein or protein composite which functions to replace gluten in gluten-free flour. The conditioned protein comprises one more non-wheat cereal storage proteins, particularly prolamins, optionally, but preferably in combination with one or more co-proteins which function to facilitate formation and stabilize formation of a protein network, e.g., β-sheet network, that facilitates retention of CO₂ for leavening. More specifically, protein composites of zein and or other non-wheat prolamins with co-proteins including casein, elastin or mixtures thereof conditioned at temperatures above the glass transition temperature of the protein mixture provide improved ingredients for preparation of gluten-free breads and other baked products. Sorghum or maize mutant flours with prolamins available for viscoelastic protein formation may also be used.

Phosphorylated glucomannan polysaccharide compositions are shown to effectively enhance healthy immune function. Dosage forms including pills, sprays, functional foods and cosmetics may achieve this benefit while being essentially free of storage protein from nongerminated seeds of Ricinus communis.

Methods and compositions for altering amino acid composition of a protein of interest are provided, particularly proteins whose three-dimensional structure is unknown. The method comprises creating interacting molecules to the native protein and selecting for engineered proteins which retain the native conformation by antibody binding. In this manner, the levels of essential amino acids in a protein can be increased yet the biological activity of the protein maintained. Also provided is an exemplary plant protein—Glycine max vegetative storage protein (VSP)—in which methionine levels have been increased.

The invention discloses a transgenic rice PA110-15 foreign insert flanking sequence and an application. According to the transgenic rice PA110-15 foreign insert flanking sequence, firstly, a 5' end sequence and a 3'flanking sequence of a transgenic rice PA110-15 strain foreign inserted gene are provided, and nucleotide sequences of the 5' end sequence and the 3'flanking sequence are represented by SEQID NO.1 and SEQID NO.2.The invention provides aPCR (polymerase chain reaction) detection primer for transgenic ricePA110-15(expressing lysine-rich storage protein gene)strain specificity and a strain specificityqualitative detection method with the flanking sequence used as a target gene. Specificityexperiments indicate that the establishedtransgenic rice PA110-15 strain specificityqualitative PCR detection method has the high specificity. Sensitivity test results indicate thatthe sensitivity of the established detection method reaches 0.1%.

The invention discloses a PCR (Polymerase Chain Reaction) qualitative detection primer and a qualitative detection method and a kit for specificity of a transgenic rice PA110-15 strain. The invention firstly provides a connection area sequence inserted with a carrier gene and flanking rice sequence in a high-lysine storage protein transgenic rice PA110-15 strain, and the base sequence is shown as SEQ ID No.2. The invention further provides the PCR primer and the qualitative detection method for the specificity of the high-lysine storage protein transgenic rice PA110-15 strain. The specificity verification experiment shows that the PCR qualitative detection method for the specificity of the high-lysine storage protein transgenic rice PA110-15 strain is high in specificity.

The present invention provides methods and compositions useful for altering the functional properties of soybean seed storage proteins. It is the novel finding of the present invention that the functional properties of seed storage proteins can be altered by reducing the expression of one or more vacuolar processing enzymes in plant seed. Accordingly, in one embodiment, the invention provides a method for altering the functional properties of one or more soybean seed storage proteins. The method comprises transforming a soybean plant cell with at least one expression cassette capable of expressing a polynucleotide that reduces the activity of a vacuolar processing enzyme in the seed of said soybean plant, regenerating a transformed plant from the transformed plant cell, and collecting seed from the regenerated transformed plant. Plants that are genetically modified or mutagenized to alter the functional properties of one or more seed storage proteins, and the transgenic seed of such plants are also provided.

The invention discloses a method for checking the purity of hybridized pepper seeds. The method is technically characterized by comprising the following steps of: soaking seeds into an acetone solution for 5-6 hours so that the seeds are degreased; taking out the seeds and airing; cutting one seed into three blocks and placing the seeds into a centrifugal tube; adding 90 microliters of extraction liquid; placing at 5 DEG C for 12 hours; extracting liquid supernatant to obtain seed storage protein extraction liquid; placing the liquid on a plate, filling glue, carrying out sample application and electrophoresis, taking the glue, dyeing and manufacturing a wafer to obtain a sample electrophoresis spectrum band; carrying out the method to respectively obtain a standard electrophoresis spectrum band and a detected sample electrophoresis spectrum band; and comparing the detected sample electrophoresis spectrum band with the standard electrophoresis spectrum band to obtain the purity of seed samples to be detected. The method disclosed by the invention is simple and convenient, rapid and accurate; and the process is easy to master and control. The method is particularly suitable for checking the purity of a hot pepper*sweet pepper type hot pepper breed combination.

The invention discloses a method for improving expression level of a target protein in transgenic rice endosperm by use of a signal peptide coding sequence of a rice storage protein. The method comprises the following steps: connecting a rice GluA-2 gene signal peptide coding sequence with a target protein coding sequence to form a fusion protein gene; transforming the fusion protein gene into rice cells to obtain transgenic rice; and culturing and ripening the transgenic rice, and then expressing the target recombinant protein in seed endosperm. In the invention, the target protein coding sequence and the GluA-2 signal peptide coding sequence containing an SEQ ID NO. 1 sequence are constructed into the fusion gene and the fusion gene is led into a plant, which can obviously improve the expression level of the target protein in the transgenic rice endosperm.