11 results about "Storage protein" patented technology

This invention discloses a molecular marker for the wheat storage protein content regulatory gene TaARF14-1D and its application. This invention belongs to the field of biotechnology, specifically relating to a molecular marker for the wheat storage protein content regulatory gene TaARF14-1D and its application. The method for identifying or assisting in the identification of wheat storage protein content according to this invention includes method A or B: A) detecting the genotype of a SNP site in the wheat genome to be tested, and identifying the wheat storage protein content based on the genotype. The SNP site is a single SNP site on the wheat 1D chromosome, with nucleotide type A or G, and is the 71st nucleotide of sequence 4; B) detecting the haplotype in the wheat genome to be tested, and identifying the wheat storage protein content based on the haplotype. The wheat variety with haplotype TaARF14-1D-Hap1 has a higher storage protein content than the wheat variety with haplotype TaARF14-1D-Hap2.

The present invention belongs to the technical field of biomedicine. Disclosed are stem-cell-derived apoptotic extracellular vesicles (ApoEVs) having an iron homeostasis regulatory effect and the use thereof in the preparation of a drug for treating iron deficiency anemia. ApoEVs are extracellular vesicles isolated from apoptotic cells derived from human periodontal ligament stem cells, and can effectively restore low intracellular iron levels induced by deferoxamine mesylate (DFOM), upregulate the expression of iron storage proteins and regulate the levels of iron export proteins, thereby improving cellular iron homeostasis. Moreover, the ApoEVs have good biocompatibility and safety, and provide a new strategy for the treatment of iron deficiency anemia.

Provided are a recombinant microorganisms having a genetic modification that increases the expression of bacterioferritin, a composition comprising the recombinant microorganism for use in reducing a nitrogen oxide concentration in a sample, and a method of reducing a nitrogen oxide concentration in a sample.

The application discloses a molecular marker and specific primer related to wheat storage protein and a plurality of spike grain number characters and application. The application belongs to the technical field of biotechnology and particularly relates to a molecular marker and specific primer related to wheat storage protein and a plurality of spike grain number characters and application. The application can be applied to identifying or assisting in identifying wheat storage protein content, spike grain number and wheat breeding by detecting the polymorphism or genotype of an InDel molecular marker in a wheat genome or detecting a substance for genotyping. The InDel molecular marker is a DNA molecule with a nucleotide sequence of 197-437 in SEQ ID No. 3 in the sequence listing. The genotype of the InDel molecular marker is identified by using a primer composition composed of SEQ ID No. 1 and SEQ ID No. 2, so that the efficiency of wheat breeding can be improved, and a dominant variety with high wheat storage protein content and spike grain number can be selected.

The application discloses a bacillus siamensis strain and application of the bacillus siamensis strain in fermentation of rapeseed meal. Bacillus siamensis The application provides the bacillus siamensis (BSJ-2) strain with a preservation number of CGMCC No.32638. The strain or culture solution or bacteria liquid or fermentation liquor thereof is applied to any one of the following or preparation of any one of the following products: 1) degradation of rapeseed meal storage protein; 2) improvement of rapeseed meal nutritional quality; 3) improvement of rapeseed meal protein utilization rate; the application finds that the bacillus siamensis (BSJ-2) strain can degrade rapeseed meal storage protein, improve rapeseed meal nutritional quality and improve rapeseed meal protein utilization rate. Bacillus siamensis The fermentation product of the bacillus siamensis (BSJ-2) strain can be used for preparing animal feed.

The invention relates to a method for increasing the oil content of soybean by using a gene polymerization module, which belongs to the field of gene engineering and biological breeding, and is characterized in that the polymerization module consisting of three transcription units is introduced into soybean to determine the influence of the polymerization module on the oil content of soybean seeds. The transgenic pyramiding breeding method relates to the improvement effect of expression of a peanut AhDGAT1 gene under the driving of three different promoters (a peanut AhDGAT1 gene promoter pAhDGAT1, a soybean storage protein gene promoter pGm7s and a soybean oil body protein gene promoter pGmOleosin) on the oil content of soybean seeds. The result shows that the expression of the AhDGAT1 driven by the three promoters can increase the oil content of the soybean seeds from 21.5% to 25.5%.

The invention discloses a molecular marker related to the content of wheat storage protein and application of the molecular marker, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to detect the content of the wheat storage protein. The molecular marker related to the wheat storage protein content disclosed by the invention is nucleotide at the 92nd site corresponding to SEQ ID No.4 in a sequence table in a wheat genome, and is C or G. The molecular marker related to the wheat storage protein content disclosed by the invention is a nucleotide at the 92nd site of SEQ ID No.4 in the sequence table. Experiments prove that the wheat molecular marker disclosed by the invention is related to the storage protein content of wheat: compared with GG genotype wheat, the storage protein content of CC genotype wheat is obviously reduced by 6.6%. The wheat molecular marker disclosed by the invention can be used for breeding wheat varieties with high storage protein content, and can be used for breeding by taking GG genotype wheat varieties as parents, so that the breeding efficiency is improved, the breeding process is accelerated, and a new possibility is provided for efficiently screening the wheat varieties with high storage protein content.

The invention discloses a molecular marker of a wheat grain storage protein regulatory gene TaMYB3R1-3D and application of the molecular marker, and belongs to the technical field of biology. The molecular marker is a group of KASP primers and comprises two reverse primers as shown in SEQ ID NO: 1 and SEQ ID NO: 2 (5'ends are respectively marked with recognition sequences of FAM and HEX fluorescent probes) and a forward primer as shown in SEQ ID NO: 3, a PHERAstar Plus multifunctional microplate reader is used for detecting a PCR (Polymerase Chain Reaction) product, and software KlusterCaller is used for carrying out genetic typing. The molecular marker has the advantages that the content of the storage protein of the wheat can be predicted by applying the KASP molecular marker developed by the invention, the cost is saved, the selection efficiency is greatly improved, the breeding process can be accelerated, and a useful molecular tool is provided for efficiently screening high-quality wheat strains.

An emulsion composition includes dry powder of dicotyledonous plant seeds in a range between 1 mass % or more and 20 mass % or less, the dry powder having an average particle size in a range between 0.2 μm or larger and 150 μm or smaller, water in a range between 20 mass % or more and 69 mass % or less, and fat and oil in a range between 21 mass % or more and 70 mass % or less.

The invention discloses a bacillus subtilis strain and application thereof in fermentation of rapeseed dregs. The invention belongs to the technical field of microorganisms, and particularly relates to bacillus subtilis capable of degrading rapeseed meal storage protein and application of the bacillus subtilis in rapeseed meal fermentation. The bacillus subtilis is bacillus subtilis BSJ-1, and the preservation number of the bacillus subtilis BSJ-1 is CGMCC (China General Microbiological Culture Collection Center) No.31314 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. Fermentation tests show that after the strain BSJ-1 and rapeseed dregs are subjected to mixed fermentation, the nutritional quality of the rapeseed dregs can be remarkably improved. The strain has a wide application prospect, and a theoretical basis is provided for popularization and application of the fermented rapeseed meal in animal feed and improvement of animal protein utilization.

The application provides a domestication gene THERMO TOLERANCE 5 (TT5) for regulating heat tolerance of plants and application thereof. In the application, a temperature-dependent Oryza minuta chromosome substitution line material for regulating rice quality and seed setting is identified, a QTL capable of polyfunctionally protecting yield and quality under high temperature is located and cloned by a method of map-based cloning, and the QTL is TT5. TT5 is a positive regulation transcription factor for heat tolerance, polyfunctionally regulates starch synthesis and storage protein accumulation, and endows high temperature tolerance. Cloning of TT5 has important significance for guiding de novo domestication of wild rice, and rapid domestication of wild rice can be realized by adding or erasing methylation of a genome.