20 results about "Storage protein" patented technology

This invention discloses a molecular marker for the wheat storage protein content regulatory gene TaARF14-1D and its application. This invention belongs to the field of biotechnology, specifically relating to a molecular marker for the wheat storage protein content regulatory gene TaARF14-1D and its application. The method for identifying or assisting in the identification of wheat storage protein content according to this invention includes method A or B: A) detecting the genotype of a SNP site in the wheat genome to be tested, and identifying the wheat storage protein content based on the genotype. The SNP site is a single SNP site on the wheat 1D chromosome, with nucleotide type A or G, and is the 71st nucleotide of sequence 4; B) detecting the haplotype in the wheat genome to be tested, and identifying the wheat storage protein content based on the haplotype. The wheat variety with haplotype TaARF14-1D-Hap1 has a higher storage protein content than the wheat variety with haplotype TaARF14-1D-Hap2.

The present invention belongs to the technical field of biomedicine. Disclosed are stem-cell-derived apoptotic extracellular vesicles (ApoEVs) having an iron homeostasis regulatory effect and the use thereof in the preparation of a drug for treating iron deficiency anemia. ApoEVs are extracellular vesicles isolated from apoptotic cells derived from human periodontal ligament stem cells, and can effectively restore low intracellular iron levels induced by deferoxamine mesylate (DFOM), upregulate the expression of iron storage proteins and regulate the levels of iron export proteins, thereby improving cellular iron homeostasis. Moreover, the ApoEVs have good biocompatibility and safety, and provide a new strategy for the treatment of iron deficiency anemia.

Provided are a recombinant microorganisms having a genetic modification that increases the expression of bacterioferritin, a composition comprising the recombinant microorganism for use in reducing a nitrogen oxide concentration in a sample, and a method of reducing a nitrogen oxide concentration in a sample.

To provide a stirrer that can reduce powder-likeness in a beverage made by mixing powdery food in water.SOLUTION: A stirrer 1 for generating a protein solution by storing and shaking protein powder and water includes: a storage part 10 for storing powder and water that extends vertically; an upper part opening / closing part 11 provided on the upper part of the storage part 10 for putting the powder and water, and discharging the protein solution; and a free spring part 13 stored inside the storage part 10. The free spring part 13 includes a coil spring 20 in which a winding diameter is constant, the pitch in the central part is dense, and the pitch on both sides in the central part is coarse, and collision bodies 21 and 22 attached to both ends of the coil spring 20, and can move slidably along a vertical direction inside the storage part 10.SELECTED DRAWING: Figure 1

The present invention provides a preparation containing an anti-Nectin-4 antibody-drug conjugate (ADC) and uses thereof. The preparation comprises an ADC, a buffer, and an excipient. The anti-Nectin-4 ADC in the preparation is stable, the protein does not aggregate even after long-term storage, the small molecule drug is less likely to separate, and the osmotic pressure of the preparation is close to isotonic, facilitating targeted therapy of Nectin-4-related diseases.

The invention discloses a molecular marker of wheat TaB3-2A2 for regulating and controlling storage protein content and application, and belongs to the technical field of molecular markers. In order to solve the technical problem of how to identify wheat with high wheat grain storage protein content or low wheat grain storage protein content, the invention provides application of a primer composition for detecting polymorphism or genotype of an SNP (Single Nucleotide Polymorphism) site in identification or auxiliary identification of the wheat grain storage protein content, the SNP site is an SNP13 site, and the SNP site is a single nucleotide polymorphism (SNP) site. The SNP 13 site is the 71st nucleotide of SEQ ID No.4, and the nucleotide type of the SNP 13 site is T or C. The SNP 13 site is the 71st nucleotide of SEQ ID No.4. The KASP molecular marker has the beneficial effects that the storage protein content of wheat can be predicted by applying the developed KASP molecular marker, so that the cost is saved, the selection efficiency is greatly improved, the breeding process can be accelerated, and a new molecular tool is provided for efficiently screening high-yield wheat varieties and cultivating.

The application discloses a molecular marker and specific primer related to wheat storage protein and a plurality of spike grain number characters and application. The application belongs to the technical field of biotechnology and particularly relates to a molecular marker and specific primer related to wheat storage protein and a plurality of spike grain number characters and application. The application can be applied to identifying or assisting in identifying wheat storage protein content, spike grain number and wheat breeding by detecting the polymorphism or genotype of an InDel molecular marker in a wheat genome or detecting a substance for genotyping. The InDel molecular marker is a DNA molecule with a nucleotide sequence of 197-437 in SEQ ID No. 3 in the sequence listing. The genotype of the InDel molecular marker is identified by using a primer composition composed of SEQ ID No. 1 and SEQ ID No. 2, so that the efficiency of wheat breeding can be improved, and a dominant variety with high wheat storage protein content and spike grain number can be selected.

The application discloses a bacillus siamensis strain and application of the bacillus siamensis strain in fermentation of rapeseed meal. Bacillus siamensis The application provides the bacillus siamensis (BSJ-2) strain with a preservation number of CGMCC No.32638. The strain or culture solution or bacteria liquid or fermentation liquor thereof is applied to any one of the following or preparation of any one of the following products: 1) degradation of rapeseed meal storage protein; 2) improvement of rapeseed meal nutritional quality; 3) improvement of rapeseed meal protein utilization rate; the application finds that the bacillus siamensis (BSJ-2) strain can degrade rapeseed meal storage protein, improve rapeseed meal nutritional quality and improve rapeseed meal protein utilization rate. Bacillus siamensis The fermentation product of the bacillus siamensis (BSJ-2) strain can be used for preparing animal feed.

The invention relates to a method for increasing the oil content of soybean by using a gene polymerization module, which belongs to the field of gene engineering and biological breeding, and is characterized in that the polymerization module consisting of three transcription units is introduced into soybean to determine the influence of the polymerization module on the oil content of soybean seeds. The transgenic pyramiding breeding method relates to the improvement effect of expression of a peanut AhDGAT1 gene under the driving of three different promoters (a peanut AhDGAT1 gene promoter pAhDGAT1, a soybean storage protein gene promoter pGm7s and a soybean oil body protein gene promoter pGmOleosin) on the oil content of soybean seeds. The result shows that the expression of the AhDGAT1 driven by the three promoters can increase the oil content of the soybean seeds from 21.5% to 25.5%.

The invention relates to application of American ginseng total protein in preparation of neuroinflammation treatment drugs. Six proteins with the highest content in the American ginseng total protein are ribonuclease-like main storage protein, main latex-like protein, phosphoglycerate kinase 1, glyceraldehyde-3-phosphate dehydrogenase, disease course related protein 10 and ribonuclease 2. The American ginseng total protein is used as an active ingredient of the neuroinflammation treatment medicine, compared with existing western medicines, the neuroinflammation treatment effect can be guaranteed, meanwhile, side effects are avoided, and the American ginseng total protein is easier to digest and absorb; compared with an existing traditional Chinese medicine composition, the composition is definite in component and controllable in quality, and interference of ineffective components is reduced.

The invention discloses a molecular marker related to the content of wheat storage protein and application of the molecular marker, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to detect the content of the wheat storage protein. The molecular marker related to the wheat storage protein content disclosed by the invention is nucleotide at the 92nd site corresponding to SEQ ID No.4 in a sequence table in a wheat genome, and is C or G. The molecular marker related to the wheat storage protein content disclosed by the invention is a nucleotide at the 92nd site of SEQ ID No.4 in the sequence table. Experiments prove that the wheat molecular marker disclosed by the invention is related to the storage protein content of wheat: compared with GG genotype wheat, the storage protein content of CC genotype wheat is obviously reduced by 6.6%. The wheat molecular marker disclosed by the invention can be used for breeding wheat varieties with high storage protein content, and can be used for breeding by taking GG genotype wheat varieties as parents, so that the breeding efficiency is improved, the breeding process is accelerated, and a new possibility is provided for efficiently screening the wheat varieties with high storage protein content.

A forage sorghum plant or plant part comprising in its genome at least one mutant allele of a kafirin storage protein gene, which encodes a polypeptide comprising SEQ ID NO: 3. The mutant forage sorghum plant of the present disclosure has improved protein digestibility thus enhancing the quality of silage produced from the plant.

Plant seeds with increased protein and methionine content and having a modified expression of a cystathionine γ-synthase (CGS) polypeptide, modified expression of a low methionine content (< about 1.0% methionine) storage protein polypeptide, or modified expression of both are provided. Methods for modifying expression of CGS polypeptides and polynucleotides and low methionine content seed storage polypeptides and polynucleotides include genome editing to modify the MTO1 regulatory region of CGS to create feedback insensitive methionine production and low methionine content polypeptides to create proteome rebalancing toward high methionine content storage protein production, and transformation with recombinant DNA constructs to enhance or suppress expression are disclosed herein.

The invention relates to application of a rice endosperm dominant expression gene IRE1 in rice quality heat resistance at natural high temperature in a field. The receptor gene IRE1 of the UPR is identified from rice, the function of the receptor gene IRE1 in forward regulation of rice quality heat resistance is successfully identified through a transgenic material, chalkiness is remarkably lower than that of a wild type by overexpressing the IRE1 gene at a high temperature, the protein content is remarkably increased, amylose is remarkably reduced, and the rice quality heat resistance is remarkably improved. The ratio of the storage protein content to the amylose content is obviously increased, and good high-temperature resistance is shown. A new gene resource is provided for rice quality breeding under the global warming background, and the gene has very important significance for cultivating high-temperature-resistant rice varieties.

The invention discloses a molecular marker of a wheat grain storage protein regulatory gene TaMYB3R1-3D and application of the molecular marker, and belongs to the technical field of biology. The molecular marker is a group of KASP primers and comprises two reverse primers as shown in SEQ ID NO: 1 and SEQ ID NO: 2 (5'ends are respectively marked with recognition sequences of FAM and HEX fluorescent probes) and a forward primer as shown in SEQ ID NO: 3, a PHERAstar Plus multifunctional microplate reader is used for detecting a PCR (Polymerase Chain Reaction) product, and software KlusterCaller is used for carrying out genetic typing. The molecular marker has the advantages that the content of the storage protein of the wheat can be predicted by applying the KASP molecular marker developed by the invention, the cost is saved, the selection efficiency is greatly improved, the breeding process can be accelerated, and a useful molecular tool is provided for efficiently screening high-quality wheat strains.

An emulsion composition includes dry powder of dicotyledonous plant seeds in a range between 1 mass % or more and 20 mass % or less, the dry powder having an average particle size in a range between 0.2 μm or larger and 150 μm or smaller, water in a range between 20 mass % or more and 69 mass % or less, and fat and oil in a range between 21 mass % or more and 70 mass % or less.

The invention discloses a bacillus subtilis strain and application thereof in fermentation of rapeseed dregs. The invention belongs to the technical field of microorganisms, and particularly relates to bacillus subtilis capable of degrading rapeseed meal storage protein and application of the bacillus subtilis in rapeseed meal fermentation. The bacillus subtilis is bacillus subtilis BSJ-1, and the preservation number of the bacillus subtilis BSJ-1 is CGMCC (China General Microbiological Culture Collection Center) No.31314 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. Fermentation tests show that after the strain BSJ-1 and rapeseed dregs are subjected to mixed fermentation, the nutritional quality of the rapeseed dregs can be remarkably improved. The strain has a wide application prospect, and a theoretical basis is provided for popularization and application of the fermented rapeseed meal in animal feed and improvement of animal protein utilization.

The application provides a domestication gene THERMO TOLERANCE 5 (TT5) for regulating heat tolerance of plants and application thereof. In the application, a temperature-dependent Oryza minuta chromosome substitution line material for regulating rice quality and seed setting is identified, a QTL capable of polyfunctionally protecting yield and quality under high temperature is located and cloned by a method of map-based cloning, and the QTL is TT5. TT5 is a positive regulation transcription factor for heat tolerance, polyfunctionally regulates starch synthesis and storage protein accumulation, and endows high temperature tolerance. Cloning of TT5 has important significance for guiding de novo domestication of wild rice, and rapid domestication of wild rice can be realized by adding or erasing methylation of a genome.

The present invention relates to the application of the QT12 gene in rice to coordinately improve rice quality and yield under natural high-temperature field environments. Map-based cloning identified the QT12 gene. High-expression alleles under high temperature disrupt the homeostasis of endosperm storage substances and negatively regulate the heat tolerance of rice quality. Large-scale field high-temperature experiments showed that non-functional or weak-functional QT12 not only confers stronger heat tolerance on quality traits such as low chalkiness, high storage protein, and low amylose content in rice grains, but also significantly increases the seed setting rate and plot yield. The G / A variation within indica rice and the 6-bp insertion / deletion variation between indica and japonica rice explain the genetic basis of heat tolerance differences within indica rice and between indica and japonica rice, such that the A variation within indica rice has lower expression and higher heat tolerance compared to G, or the deletion of 6 bp within indica rice has lower expression and higher heat tolerance compared to the insertion of 6 bp within japonica rice, which is of great significance for breaking through the breeding bottleneck of the contradiction between crop resistance and growth, and between yield and quality.

The present invention discloses a molecular marker and specific primers for wheat storage protein, starch and grain weight regulatory gene TaB3-2A1 and its application. The present invention provides a material for detecting the genotype of the 523rd SNP site of the TaB3-2A1 gene in the wheat genome for use in identifying or assisting in identifying the storage protein content and / or starch content and / or 1000-grain weight of the wheat grain to be tested; the SNP site is the 523rd nucleotide of the DNA molecule shown in Sequence 4 of the sequence table. Experiments of the present invention have shown that TaB3-2A1-KASP 523A / T Molecular markers have a good correlation with grain storage protein content, starch content, and grain weight phenotypes, and can be used as molecular markers for grain storage protein content, starch content, and grain weight phenotypes to assist in wheat germplasm improvement. This identification can better meet the needs of wheat quality improvement and meet the needs of the public on a larger scale.