341 results about "Amino acid composition" patented technology

Compositions and methods for administering ketone bodies to a subject include beta-hydroxybutyrate complexed with or coupled to at least one amino acid. The compositions and methods cause one or more of: weight loss, weight maintenance, reduced blood glucose level, maintenance of blood glucose level, increased muscle and physical performance, enhanced metabolic and cellular repair, improved detoxification and gut health, targeted bioavailability, sustained release and controlled absorption, increased metabolic efficiency, enhanced cellular uptake, minimized side effects, multifunctional therapeutics, improved focus, improved energy, improved cognitive function, improved mental acuity, treatment of traumatic brain injury, treatment of diabetes, treatment of neurological disorder, treatment of cancer, treatment of inflammatory condition, appetite suppression, anti-aging effects, anti-glycation effects, treatment of epilepsy, treatment of depression, improved performance, improved muscle mass, improved motor function, increased strength, increased metabolism, increased fat loss, increased fat oxidation, improved body composition, and improved mood.

The invention provides copper-loaded duck-origin lactobacillus plantarum with high antibacterial peptide yield and application of the copper-loaded duck-origin lactobacillus plantarum. The preservation number of the provided copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The domesticated and bred duck-origin lactobacillus plantarum with high antibacterial peptide yield can tolerate high-concentration copper ions, the domesticated and bred lactobacillus plantarum can stably grow, and the contents of antibacterial peptide, extracellular protein, lipase, amylase, lactic acid, acetic acid and the like in the domesticated and bred lactobacillus plantarum strain are increased; and the amino acid composition is changed. The strain can convert inorganic copper into organic copper, the utilization rate of copper is increased, and growth and development of organisms are promoted. Moreover, small peptides and extracellular proteins in metabiotics generated after fermentation have the effect of effectively inhibiting harmful bacteria such as riemerella anatipestifer, escherichia coli, salmonella and staphylococcus of poultry, and are beneficial to eliminating intestinal inflammatory factors, maintaining intestinal health and improving the feed utilization rate.

The invention provides a micromolecular recombinant XVII type collagen as well as a preparation method and application thereof, and relates to the technical field of protein engineering. The novel recombinant human XVII type collagen is obtained by optimizing and screening a plurality of spiral region sequences such as a collagen structural domain, a C-terminal region and a middle region of the human XVII type collagen. The amino acid composition of the recombinant XVII type collagen is consistent with that of a natural collagen alpha1 chain, immunological rejection is not generated when the recombinant XVII type collagen is applied to a human body, the recombinant XVII type collagen can be widely applied to surgical medical treatment and medical beauty industries, the molecular weight of the collagen is small, efficient secretion soluble expression in eukaryotic host cells such as pichia pastoris can be achieved, and the recombinant XVII type collagen has a good application prospect. And industrial large-scale production is carried out. According to the present invention, the verification results show that the micromolecular recombinant XVII type collagen further has basement membrane anti-aging activity, wherein the basement membrane anti-aging activity specifically comprises the improvement of the expression levels of the IV type collagen, the VII type collagen and the XVII type collagen;

The invention provides an amino acid frosted cleaning composition as well as a preparation method and application thereof. The amino acid frosted cleaning composition comprises a surfactant, an amino acid composition, a thickening agent, a humectant, an antioxidant, a pH regulator and water, the surfactant is a combination of cocoyl glycinate, cocamidopropyl hydroxy sulfobetaine, sodium cocoyl methyl taurine taurate, sulfonic acid hydroxypropyl ester lauryl glucoside cross-linked polymer sodium and glycolipid. According to the invention, a mild surfactant system with amino acid as a main component is adopted, so that the shampoo has rich and fine foam, quick foaming performance and easy washing performance, and the skin feeling is smooth and soft after washing. In addition, the system contains suspended crystal frosted particles formed by self-assembly of the amino acid composition, the appearance is novel, the better efficacy performance is achieved, and meanwhile the cleaning effect and the use comfort are improved.

The invention relates to the field of food, and provides a soybean protein product and a preparation method thereof.The method comprises the steps that soybean and water are subjected to pulp grinding and centrifugal treatment, and residue-removed soybean milk and soybean residues are obtained; performing cold precipitation and centrifugal treatment on the residue-removed soybean milk to obtain a cold precipitation precipitate and a cold precipitation supernatant; carrying out ultrafiltration treatment on the cold precipitation supernatant, and collecting retentate; respectively drying the cold precipitation precipitate and the intercepted substance to obtain a cold precipitation dried substance and an intercepted dried substance; and carrying out mixing treatment on the cold-precipitated dried substance and the intercepted dried substance to obtain the soybean protein product. The soybean protein product prepared by the method contains all amino acids essential to human bodies, the proportion of the amino acids is close to that of ideal amino acids, and the nutritional value is high. Moreover, the method is simple and convenient to operate, low in cost and suitable for large-scale production.

The design consists of a rectangular shape on the left side, excluding the dotted lines; in the center of the drawing, there is an arched window-shaped feature with a curved upper portion; floral patterns are arranged along the border of the arched window, and a cow's face is depicted at the bottom of the drawing; the nutrition facts and full amino acid in the drawing form no part of the claimed design.

The invention discloses a saline-alkali soil rhizosphere microdomain soil improvement and fertilization fertilizer and a preparation method thereof, and belongs to the technical field of saline-alkali soil treatment. The fertilizer is composed of a microbial inner core, a slow release fertilizer secondary outer layer and a soil conditioner outer layer, the microbial inner core is composed of a compound microbial agent (composed of nitrogen-fixing bacteria, phosphate solubilizing bacteria and live potassium bacteria), biochar and a polymer organic membrane material, the slow release fertilizer secondary outer layer is composed of a chemical fertilizer, an inhibitor (a nitrification inhibitor and / or a urease inhibitor) and amino acid, and the soil conditioner outer layer is composed of a soil conditioner outer layer. The soil conditioner outer layer is composed of a soil conditioner special for saline-alkali soil improvement. The method has the beneficial effects that (1) the pH value of the soil is adjusted and the soil structure is improved, so that the microbial flora can better adapt to the soil environment of the saline-alkali soil; and (2) the biochar and the isolating membrane effectively protect the activity of microorganisms, and the microorganisms are released when the pH value and the salt-based ions of the soil around the rhizosphere reach a lower level, so that the saline-alkaline obstacle of the growth of the microorganisms is overcome.

The invention provides an amino acid water-soluble fertilizer as well as a preparation method and application thereof, and relates to the technical field of amino acid water-soluble fertilizers. The amino acid water-soluble fertilizer comprises compound amino acid, a microbial fermentation product, inorganic salt, a surfactant, a dispersing agent and a preservative, the microbial fermentation product is obtained by aerobic fermentation of radix puerariae and rhizoma atractylodis through a complex microbial inoculant; the compound amino acid is composed of animal protein hydrolyzed amino acid and vegetable protein hydrolyzed amino acid. According to the water-soluble fertilizer disclosed by the invention, through the synergistic interaction of the compound amino acid and the microbial fermentation product, multi-objective optimization of nutrition supply, stress resistance regulation and metabolism enhancement is realized, the yield and the quality of crops are remarkably improved, an efficient and environment-friendly solution for increasing the yield and improving the quality is provided for the crops, and the water-soluble fertilizer has a remarkable agricultural application value.

Semisolid supplement compositions are disclosed comprising at least one non-ester nitrate compound and at least one isolated amino acid compound. The at least one isolated amino acid compound is a separate compound than the at least one non-ester nitrate compound. The composition contains at least about 0.05625 mg nitrate ion (NO3−).

The invention belongs to the technical field of food flavor engineering, and discloses a Maotai-flavor Baijiu flavor composite essence based on a non-volatile substance release promoting effect, and the composite essence is composed of a volatile aroma substance, a non-volatile organic acid and an amino acid. Aiming at the technical bottlenecks of rapid attenuation of aroma intensity of volatile components and single flavor dimension in the existing Maotai-flavor Baijiu flavor compound essence blending technology, the invention creatively constructs a molecular synergistic system of volatile and non-volatile flavor components. By adding non-volatile substances, the effect of enhancing the flavor of the Maotai-flavor Baijiu flavor composite essence is achieved. The technology not only completely reproduces the flavor structure of the traditional solid-state fermented Maotai-flavor liquor, but also endows the compound liquor and flavor foods (such as Maotai-flavor baked products, compound seasonings and non-alcohol ready-to-drink beverages) with a continuous and stable aroma releasing characteristic, and provides a molecular-level regulation and control scheme for upgrading the sensory quality of liquor flavor standardization and food industrial production.

The invention discloses a method for impregnating coffee beans with fructose under the assistance of ultrasonic waves, and relates to the technical field of food processing, and the method is technically characterized by comprising the following steps: selecting Robursta coffee beans with the moisture content of 10-12%; mixing with a 5% fructose solution according to a solid-liquid ratio of 1: 4, treating for 5-20 minutes (preferably 5 minutes) under ultrasonic waves of 40 kHz and 120 W, and totally impregnating for 4 hours; performing forced air drying at 40 DEG C for 23h until the water content is 10- And baking at the temperature of 175 DEG C for 7-8 minutes. A honeycomb net structure is formed on the surface of the coffee beans through ultrasonic treatment, the total sugar content is remarkably increased to 54.65 mg / g, amino acid composition is affected, and bioactive substances such as polyphenol and flavone are increased. The score of the baked coffee beans is more than 72 (the score of the coffee beans is 74.92 after the coffee beans are subjected to ultrasonic treatment for 5 minutes) through an SCA cup test, and the coffee beans have caramel fragrance, nut fragrance and smoother mouth feel. The method is simple and convenient to operate, low in cost and capable of effectively improving the flavor quality of the Robursta coffee.

The invention relates to the technical field of biological medicines, and discloses a polypeptide for treating osteoarthritis by aiming at AHR (Aryl Hydrocarbon Receptor), the amino acid sequence of the polypeptide is GFQQSDVIHQSVYELISINHTEDRA, and the amino acid sequence of the polypeptide is GFQQSDVIHQSVYELISINHTEDRA, and the amino acid sequence of the polypeptide is GFQQSDVIHQSVYELISINHTEDRA. The polypeptide is prepared by a solid-phase polypeptide synthesis method; the solid-phase polypeptide synthesis method specifically comprises the following steps: synthesizing from a C end to an N end, connecting a carboxyl group of a first amino acid to insoluble resin, then adding other amino acids one by one to form a peptide chain, and carrying out protection and deprotection reactions of a side chain in each step. The polypeptide is composed of natural amino acids and has good biocompatibility and degradability, and experimental results show that no obvious toxic reaction occurs in the cell level and the animal level, so that the polypeptide has conditions for further development of pharmacokinetic research and preclinical tests, and a feasible basis is provided for conversion to clinical application.

The invention discloses an amino acid composition for treating alopecia and application of the amino acid composition in preparation of a medicine for treating alopecia, and belongs to the field of medicine configuration products, the amino acid composition is composed of a targeting core intervention component and an auxiliary strengthening synergistic component, the targeting core intervention component is prepared from 30-50 parts of arginine, 50-155 parts of lysine and 50-120 parts of glutamic acid, and the auxiliary strengthening synergistic component is prepared from an auxiliary strengthening synergistic component and an auxiliary strengthening synergistic component. 10 to 40 parts of cysteine and 20 to 60 parts of leucine; the auxiliary strengthening synergistic component is prepared from 15 to 45 parts of tyrosine, 15 to 40 parts of glycine, 10 to 40 parts of glutamine, 20 to 35 parts of serine, 5 to 25 parts of alanine, 2 to 20 parts of aspartic acid and 15 to 35 parts of phenylalanine; the composition can be used as an active ingredient to be prepared into different dosage forms such as a pigmentum, a spray, an ointment and a liniment, is applied to prevention of alopecia and promotion of hair growth, and has a remarkable treatment effect.

This invention discloses a soil improvement and fertilization fertilizer for rhizosphere micro-domains in saline-alkali land and its preparation method, belonging to the field of saline-alkali land management technology. The fertilizer consists of a microbial core, a slow-release fertilizer sub-outer layer, and a soil conditioner outer layer. The microbial core is composed of a compound microbial agent (composed of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, and potassium-active bacteria), biochar, and a high-molecular organic membrane material. The slow-release fertilizer sub-outer layer is composed of chemical fertilizers, inhibitors (nitrification inhibitors and / or urease inhibitors), and amino acids. The soil conditioner outer layer is composed of a soil conditioner specifically for saline-alkali land improvement. The advantages of this invention are: (1) it first adjusts the soil pH and improves the soil structure, so that the microbial community can better adapt to the soil environment of saline-alkali land; (2) the biochar and the isolation membrane effectively protect the microbial activity, and release the microorganisms when the soil pH and base ions around the rhizosphere reach a low level, overcoming the saline-alkali barrier to microbial growth.

The invention discloses a litchi disease-resistant gene LcEXP1 and an application thereof. The litchi disease-resistant gene LcEXP1 is separated from litchis, the full-length cDNA (complementary deoxyribonucleic acid) of the gene is 750bp, and the encoded protein is an expansion protein with the full length consisting of 250 amino acids. The litchi disease resistance related gene LcEXP1 is transiently expressed in tobacco, different tobacco leaves are inoculated with phytophthora capsici and alternaria alternata after two days, it can be observed that after expression of the LcEXP1, scabs are remarkably reduced compared with negative control, and biostatistical analysis shows that the areas of the two treated scabs are remarkably different. Therefore, the gene can effectively promote the disease resistance of plants such as tobacco and the like, and has a wide application prospect in developing products for improving the disease resistance of the plants.

The invention relates to the technical field of dihydroquercetin preparation, in particular to a method for preparing dihydroquercetin from larch sawdust, which comprises the following steps: S1, raw material pretreatment: selecting roots of larch sawdust as a raw material, and pretreating the raw material to obtain a base material; s2, strain preparation: the strain is prepared, and the strain is used for fermentation treatment of the base material. According to the method, the white-rot fungi can rapidly grow and secrete a large amount of enzymes for decomposing lignin substances, so that the decomposition efficiency of larch wood chips is improved, the yield of dihydroquercetin is increased, the culture medium composed of soybean sprout juice, glucose and amino acid provides rich carbon and nitrogen nutrient substances for the white-rot fungi, the growth and metabolism requirements of the white-rot fungi are met, and the yield of the white-rot fungi is increased. The larch sawdust can generate more enzymes to decompose substances in the sawdust and promote the generation of dihydroquercetin, so that the purity and the yield of dihydroquercetin prepared from the larch sawdust are effectively improved.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for promoting secondary flowering of crape myrtle, the regulator comprises 50 parts by weight of benzylaminopurine, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition; or 18 parts by weight of benzylaminopurine, 18 parts by weight of gibberellin, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition. The regulator provided by the invention comprehensively acts on multiple aspects of cell division, dormancy breaking, hormone regulation, nutrition supply, stress resistance enhancement and the like to jointly promote secondary blooming of the crape myrtle, increase the blooming amount and prolong the blooming period, and by virtue of multiple aspects of effects of pruning, fertilization, insect killing, plant growth regulator regulation and the like, the yield of the crape myrtle is increased. Secondary blooming of the crape myrtle is promoted, the blooming rate, the blooming duration and the blooming amount of secondary blooming are increased, and the growth vigor of the crape myrtle in the next year is not affected.

The present invention relates to a composition for enhancing or protecting muscles, comprising a fermented salmon roe product. According to the present invention, if salmon roe is fermented, the amino acid composition of proteins changes, and the effects of promoting muscle synthesis, inhibiting muscle degradation and damage, and protecting muscles are greatly improved. Therefore, the fermented salmon roe product of the present invention can be applied to food to exhibit effects of enhancing and protecting muscles, and can also be used as a pharmaceutical composition for preventing or treating sarcopenia or amyotrophic diseases through the effects.

The objective of the present invention is to provide a peptide having ACE inhibitory activity and a composition thereof. [Solution] The present invention discloses a peptide with ACE inhibitory activity and a composition thereof. The peptide is composed of three amino acids and has the ability to inhibit ACE activity. Therefore, by administering an effective amount of the peptide disclosed in the present invention or a composition containing the peptide to an individual, the individual's blood pressure can be effectively adjusted to approach normal values, thereby achieving the effect of preventing and / or treating hypertension and its related diseases.

The invention belongs to the field of functional polypeptide preparation, and particularly relates to euphausia superba peptide and application thereof in preparation of immunomodulatory drugs. The euphausia superba peptide is prepared from the following peptide fragments: DIFDPL (Diaphragmitic Induced Factor Dinucleotide Lipoprotein Lipoprotein), KPWALT (Keylanine White Amplified Transcription), LPFQRL (Lipoprotein Lipoprotein Quantitative Reducing Lipoprotein), FYFF (Factor Yeast Factor The Antarctic krill mixed peptide sample prepared by the invention has no cytotoxicity, and can promote cell proliferation, enhance cell NO secretion, improve cell phagocytic ability and effectively enhance immunity. The euphausia superba peptide prepared in the invention is easier to absorb, and has significant reduction and oxidation resistance. The polypeptide provided by the invention is a functional influence factor which has huge potential in the aspect of enhancing immunity, different bioactive peptides have different action mechanisms due to amino acid composition and structural characteristics, and theoretical support is provided for further developing food for enhancing the immunity function by utilizing the euphausia superba peptide. The development value of the euphausia superba is improved.

A solid supplement composition comprising at least one non-ester nitrate compound and at least one isolated amino acid compound, wherein the at least one isolated amino acid compound is citrulline, wherein the at least one isolated amino acid compound is a separate compound than the at least one non-ester nitrate compound, and wherein the solid supplement composition comprises an amount of nitrate ion (NO3—) that is at least 0.33125 mg.

The invention provides an allosteric nanogel adjuvant as well as a preparation method and application thereof, and belongs to the technical field of vaccines. The allosteric nanogel adjuvant comprises a self-assembly motif, a responsive site and an immunomodulatory peptide fragment, and the self-assembly motif is composed of 2-5 amino acids; the allosteric nanogel adjuvant has a structure as shown in a formula I, and is named as NTP5 for short; the allosteric nanogel adjuvant is self-assembled into nanoparticles, the nanoparticles are allosteric into a nanofiber structure through responsive broken bonds, nanogel is further formed, and the key problems of a traditional vaccine in the aspects of antigen delivery and immune activation are effectively solved. The nanogel adjuvant can efficiently load various antigens to form a stable nano vaccine, activate an immune microenvironment and provide more means and thoughts for vaccine development.

Disclosed herein are boronated amino acid compositions comprising tyrosine derivatives TC220 and TC221 and improved methods of making TC220 and TC221. Thus, the TC220 and / or TC221 can be expanded to commercial scale and administered to patients as neutron trapping agents, and methods of treating cancer, immune disorders, and other diseases by utilizing a neutron capture therapy pattern are provided.

The invention discloses a method for detecting polypeptide sequence difference and post-translational modification thereof based on a nanopore system, the nanopore system is constructed in the method, and the nanopore system comprises an alpha-hemolysin nanopore and octa-amino modified gamma-cyclodextrin embedded in the nanopore; the amino acid sequence of the alpha-hemolysin nanopore comprises mutation sites M113R and T145R, and the alpha-hemolysin nanopore is characterized in that the alpha-hemolysin nanopore According to the detection method, polypeptide with negative charges enters a nanopore under the driving of external voltage, an ion current blocking signal is recorded, and the polypeptide sequence difference and post-translational modification are analyzed according to blocking amplitude, duration, noise characteristics and event frequency. The method can detect a peptide chain with the length of 2-10 amino acid residues, can distinguish the post-translational modification state in homopolypeptide composed of 4-10 amino acids and polypeptide by single amino acid resolution, and can be used for protein enzyme digestion product analysis, oligopeptide drug detection and rapid polypeptide identification under the condition of no mass spectrum.

The invention discloses a compound vitamin amino acid premix as well as a preparation method and application thereof, and belongs to the technical field of feed additives. An embedding system is formed by the chitosan, the polyethylene glycol and the glyceryl ricinoleate, and the tea tree oil and the perilla oil are compounded to serve as an antioxidant, so that the stability of vitamins can be remarkably improved. Besides, hydroxyapatite is added into the starch, modification treatment is performed through succinic anhydride and polyvinylpyrrolidone, the component compatibility is good, o-vanillin is introduced, the fragrance of o-vanillin has a food calling effect, the palatability is improved, the components of the functional additive are optimized, and the remarkable immune enhancement and growth promotion effects can be achieved; the cellular immunity and humoral immunity functions of the body are enhanced, and the granulating formability is effectively promoted by combining with the components of the carrier with good compatibility. On the whole, through interaction of the vitamin composition, the amino acid composition, the functional additive and the carrier, the immunity and growth performance of animals are jointly improved from multiple aspects.

The invention discloses an N-terminal protection sequence for improving the stability of 7 beta-hydroxysteroid dehydrogenase and an efficient preparation method of a fusion protein, and belongs to the technical field of biology. An N-terminal protection sequence capable of remarkably improving the stability of the 7beta-HSDH is designed and synthesized, the protection sequence is composed of 45 amino acids, and the stability of the 7beta-HSDH can be effectively improved by spirally embedding an N-terminal alpha exposed to the outside of the original 7beta-HSDH through a section of inflection structure. Compared with the original 7beta-HSDH, the half-life period of the enzyme mutant N45-7beta-HSDH added with the protection sequence is prolonged by 2.8 times under the catalytic conditions that the pH is 8.0 and the temperature is 30 DEG C. When the N45-7beta-HSDH is used for catalyzing 7-KLCA to generate UDCA, the enzyme dosage is reduced by 45.5%, the molar conversion rate is as high as 99.41%, the production cost is greatly reduced, and the N45-7beta-HSDH has actual industrial production value.

The present application provides a bionic antibacterial peptide, a pharmaceutical composition and its application in the preparation of antibacterial drugs, as well as a design method of the bionic antibacterial peptide. A variety of compounds are used to modify the N-terminus of a polypeptide sequence composed of 20 natural amino acids, thereby generating a novel antibacterial peptide with bionic functions. Moreover, a method based on the interaction between deep learning and experiments is adopted to screen the designed bionic antibacterial peptide. The time, manpower, material resources and financial resources consumed are greatly reduced compared with traditional drug screening. In addition, the designed novel bionic antibacterial peptide has good biocompatibility. The functional groups modified at the N-terminus can activate the self-assembly activity of the polypeptide and help the polypeptide target the bacterial membrane, which is not easy to cause bacterial drug resistance, can effectively kill drug-resistant bacteria, has a faster bactericidal speed, and has potential and broad application prospects in the treatment of clinical bacterial and drug-resistant bacterial infectious diseases.

The invention discloses a multi-source composite peptide instant solid beverage and an enzymolysis preparation method thereof, and belongs to the technical field of functional food processing.The beverage is prepared by taking animal, plant, microorganism, insect and algae five-element composite protein sources as raw materials, conducting enzymolysis and then compounding with functional auxiliary materials, a flavoring agent and a stabilizer. Wherein the active peptide fragment is high in proportion and is modified by functional groups. The preparation method comprises the following steps: crushing the multi-element protein, carrying out ultrasonic pretreatment, carrying out enzymolysis by adopting a compound enzyme system containing multiple proteases, carrying out enzyme deactivation and nanofiltration graded purification, and carrying out supercritical COspray drying or freeze drying to obtain the solid beverage. The problems that a traditional peptide beverage is single in protein source, low in biological activity, poor in instant solubility and the like are solved, the obtained product is comprehensive in amino acid composition and has the characteristics of rapid cold water instant dissolution, long-term stability and non-layering, a synergistic effect is formed among functional components, the absorption efficiency and physiological functions such as immune regulation and oxidation resistance are remarkably improved, and the beverage is suitable for large-scale production. Wide industrial application prospects are realized.

The present application relates to oxidized squalene cyclase, recombinant vector, recombinant engineering bacteria and application thereof, and preparation method of parkol. The oxidized squalene cyclase comprises: a polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1; or a polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1 with one or more amino acids being deleted, substituted or added; or a polypeptide having at least 80% homology with the polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1. The above-mentioned oxidized squalene cyclase can catalyze 2, 3 oxidized squalene to produce parkol, and the enzyme can be expressed according to the coding sequence of the cyclase, and then the substrate is catalyzed to improve the yield of parkol.

The invention provides a method for analyzing polypeptide or protein in a to-be-detected sample, which comprises the following steps of: separating by reversed-phase high-performance liquid chromatography, measuring the content of free amino acid before and after hydrolysis of the to-be-detected sample by a detector, determining the amino acid composition of the polypeptide or protein according to the difference value, and indirectly reflecting the content of polypeptide or protein impurities. The analysis method disclosed by the invention has the characteristics of simplicity in operation, strong specificity, high accuracy, good reproducibility and the like, and is successfully applied to analysis research and quality control of residual polypeptide impurities in raw material medicines of levodopa and lacosamide.