213 results about "Glycation" patented technology

The invention discloses an abdominal aortic aneurysm progress prediction method and system based on cross-modal knowledge distillation. The method comprises the following steps: collecting blood of a target object; the method comprises the following steps: detecting the concentration of blood protein fingerprint markers of blood of a target object, wherein the blood protein fingerprint markers comprise matrix metalloproteinase-12, calcitonin-related polypeptide-alpha, uromodulin, nerve injury induction protein-1, glycosylated hemoglobin A1c and fibroblast growth factor-9; carrying out abdominal aortic aneurysm risk calculation by calling Student-net of cross-modal knowledge distillation based on the blood protein fingerprint marker concentration; wherein the input of the Teaser-net of the cross-modal knowledge distillation comprises a CTA image feature and a pathological section feature, and a category probability vector output by the Student-net is aligned with a category probability vector output by the Teaser-net; and outputting the risk level of the abdominal aortic aneurysm and the probability of occurrence / progress / rupture of the abdominal aortic aneurysm in the preset year. And the development of the aortic aneurysm disease is accurately predicted.

The invention discloses a diabetic complication monitoring auxiliary system and method in the technical field of complication monitoring, and the system comprises a data collection module which is used for obtaining local multi-modal data of a user in real time; the federal learning module is used for integrating the local multi-modal data of the user with other anonymous medical databases through a distributed architecture and constructing a multi-family cross-institution complication prediction model; the causal reasoning module is used for analyzing the causal relationship among the blood glucose fluctuation, the glycosylated hemoglobin and the target complication based on an anti-factual causal reasoning algorithm, and outputting a causal effect value to correct the prediction deviation; the prediction module is used for generating occurrence probabilities of future specific complications based on the causal effect values and risk scores output by the complication prediction model, and classifying the occurrence probabilities according to risk levels; and the early warning module is used for generating personalized intervention suggestions according to the risk levels. According to the scheme, the data leakage risk in the data acquisition process is reduced through the federal learning distributed architecture, and the prediction reliability is improved.

The invention relates to the technical field of medical informatics, in particular to an AI-based intelligent early warning system for hyperuricemia male reproductive metabolism abnormality. The system comprises a memory and a processor, and the processor executes a computer program stored in the memory to realize the following steps: acquiring uric acid data, waistline data, glycosylated hemoglobin data and reproductive index data of a target male; combining the uric acid fluctuation abnormality degree, the multi-dimensional blood glucose abnormality expression degree and the movement rule expression degree of each stage to obtain the reproduction metabolism abnormality degree; according to the relative relationship between the reproductive index data and the corresponding standard range, obtaining the abnormal performance degree of the reproductive index; integrating the reproductive metabolism abnormality degree and the reproductive index abnormality expression degree to obtain a multi-dimensional feature linkage reproductive metabolism expression comprehensive evaluation degree of the target male; and according to the comprehensive evaluation degree of the multi-dimensional feature linkage reproductive metabolism performance, whether early warning is carried out is judged. According to the invention, the accuracy and credibility of intelligent early warning of the risk of abnormal male reproductive metabolism of hyperuricemia are improved.

The invention provides a recognition and early warning method and device for irritable hyperglycemia, electronic equipment and a storage medium, and is applied to the technical field of medical data processing. According to the method, data type information (including FreeStyle LibreH dynamic blood glucose and glycosylated hemoglobin data), influence factor data (improved and preset irritable hyperglycemia index and the like) and abnormal state information (fasting interference and detection difference) are taken; acute-stage and chronic-stage average blood glucose basic data are generated through preprocessing. The index weight is enhanced and improved by means of a dynamic fusion engine, the probability is predicted by combining space-time correlation analysis and binary logistic regression, and the abnormal characteristics are obtained by comparing the difference of the AUC by adopting Delog test. The multi-modal features are processed to obtain an identification result, clinical indexes screened by LASSO are fused, index differences are compared to generate risk influence factors, finally, a dynamic early warning result is generated based on a space-time correlation early warning engine and a multi-target strategy, and accurate identification and early warning are achieved.

Methods of treating, preventing and / or inhibiting one or more of the symptoms of a respiratory viral infection in a subject by administering a therapeutically effective amount of a glycated chitosan (GC) polymer with characteristics as disclosed herein or a vaccine composition comprising such GC polymer in combination with one or more viral antigens are provided. Methods of reducing morbidity and / or mortality of a respiratory viral infection in a subject, methods of inducing an innate immune response in mucosa of a subject, methods of generating mucosal secretory IgA antibodies and / or neutralizing serum IgG antibodies in a subject, and methods of vaccinating a subject against a respiratory viral infection, wherein these methods comprise administering to the subject a therapeutically effective amount of a glycated chitosan (GC) polymer as disclosed herein or a vaccine composition comprising such GC polymer in combination with one or more viral antigens are further provided.

The invention provides a sugar fat disease death risk prediction method and system based on NHANES data, and the method comprises the steps: obtaining the fasting blood glucose FPG and glycosylated hemoglobin HbA1c clinical detection data of a sugar fat disease patient, and calculating an SHR value through a formula; based on the data of the NHANES database, SHR total cause bilateral critical values of total cause death rate and cardiovascular death rate, SHR cardiovascular bilateral critical values and SHR cancer unilateral critical values of cancer death rate are obtained; and comparing the SHR value with an SHR total cause double-side critical value, an SHR cardiovascular double-side critical value and an SHR cancer single-side critical value of the cancer death rate, and carrying out risk layering and evaluation. The biochemical aging acceleration index has a prediction effect on the diabetes death risk, and KDMAgeAccel significantly intermediates the death risk caused by SHR.

The invention belongs to the technical field of cosmetics, and particularly relates to a cell energy activation composition with an anti-aging effect and a preparation method thereof, the cell energy activation composition with the anti-aging effect and the preparation method thereof are characterized in that the cell energy activation composition with the anti-aging effect comprises the following components: 3-10 parts of thiotaurine and 0.01-0.5 part of a metal ion compound, the composition is prepared from the following raw materials in parts by weight: 0.05-0.5 part of an antioxidant, 0.005-0.05 part of redox regulatory protein, 0.1-6 parts of a raw grass-containing extract, 0.01-1 part of 4-tert-butyl cyclohexanol and 0.05-3 parts of a lipidosome delivery system, according to the invention, multi-channel energy activation is carried out: thiotaurine participates in energy metabolism, metal ions promote enzyme activity, thioredoxin maintains redox balance, and mitochondrial functions are comprehensively improved; liposomal delivery enhances absorption: the transdermal ability of active matters is improved, the stability is enhanced, and the utilization rate is increased; multiple anti-aging mechanisms are as follows: oxidation resistance, saccharification resistance and cell repair.

Described are a recombinant host cell, a composition comprising a glucoamylase and methods of saccharifying the starch substrate using the glucoamylase. Moreover, the disclosure also relates to a process of producing fermentation products and a method for increasing starch digestibility in an animal as well as a method of producing a fermented beverage.

The invention provides a chromatographic detection method capable of accurately detecting the saccharification level of an antibody, the method is used for detecting through a boric acid affinity column, factors influencing the accuracy of a boric acid affinity chromatography (BAC) method of the monoclonal antibody are deeply discussed, reasons for inaccurate saccharification detection of the BAC method of the monoclonal antibody are clarified, an improved BAC method is successfully developed, and the method can be used for detecting the saccharification level of the monoclonal antibody. The saccharification level of infliximab can be measured more accurately, and the accuracy of the method is confirmed through a rapid multi-attribute method based on mass spectrum. In addition, by testing different types of antibody drugs, the wide applicability of the method in conventional antibody drug saccharification detection is proved. The method provides a new rapid and accurate technical scheme for quality control of antibody drugs. The research results are helpful for promoting the improvement of related technical platforms and the updating of concepts, so that the product development and quality control capabilities are enhanced.

The invention relates to a multi-target anti-aging transdermal penetration enhancing composition based on synergistic interaction of PDRN and natural fermentation and application of the multi-target anti-aging transdermal penetration enhancing composition. The composition comprises saccharomycetes / rice fermentation product filtrate, kelp extract and PDRN in a mass ratio of (0.1-20): (0.05-4): (0.01-3), and is prepared according to the following steps: adding a solvent and a humectant into a container, and continuously stirring until the solvent and the humectant are completely dissolved; adding a chelating agent and a skin conditioner, and performing heat-preservation stirring until a system is uniform and transparent; adding the emulsifier, preserving heat and stirring until the emulsifier is completely dissolved; stopping heating, cooling, adding the functional components and the preservative, and stirring until the components are uniformly mixed; and quantitatively filling in a sterile environment. Compared with the prior art, the hydration environment of the yeast / rice fermentation product filtrate improves the transdermal efficiency of the kelp extract and PDRN; the kelp extract eliminates ROS, resists saccharification and reduces oxidative damage; the deep repair function of the PDRN reversely enhances the epidermis repair effect of the PDRN and the PDRN, and a synergistic effect of '1 + 1 + 1gt; 3' is formed.

The application belongs to the technical field of biological medicine, and particularly relates to a preparation method and application of a styrene microsphere for glycosylated hemoglobin detection. The styrene microsphere is prepared through a dispersion polymerization method, a seed polymerization method and a preparation step of organic high molecular particles. The microsphere prepared in the application has moderate particle size and concentrated distribution, good uniformity of the microsphere particle size, a hydroxyl covering on the surface of the microsphere to form a hydrophilic layer, and can effectively reduce the matrix effect, so as to achieve accurate analysis of glycosylated hemoglobin HbA1c. The result is accurate, stable and small in error in the detection, and the application can be applied to quantitative detection of diabetes.

The invention relates to the technical field of gelators and functional cosmetics, in particular to a porphyrin core-based gelator, preparation thereof and application of the porphyrin core-based gelator in preparation of products for removing skin glycated toxins. The gel factor provided by the invention is a C2 symmetric chiral gel factor: L-Por-Phe. A central core (porphyrin ring) of the gelator provides strong hydrophobic acting force and pi-pi stacking driving force, is not only a driving core for supramolecular self-assembly, but also is a key site for adsorbing aromatic AGEs toxins; a natural chiral source and an additional benzene ring side group are introduced into a side chain, the hydrophilic-hydrophobic balance of molecules is adjusted, and a supramolecular assembly is induced to form a single chiral spiral nanofiber; the connecting arms provide intermolecular hydrogen bonds to stabilize the three-dimensional network structure of the hydrogel; the terminal group endows the molecule with pH responsiveness, so that the gel can maintain a stable gel state in a skin physiological pH environment, and has good moisture retention.

The application discloses a kind of multi-effect repair type collagen and its application in the technical field of synthetic biology, the application selects transdermal peptide TD-1, the coding gene of the transmembrane region of human XVII type collagen, extracellular sixteenth non-collagen region and extracellular fifteenth collagen region and His tag is connected in series, and gene sequence is optimized by pichia pastoris codon selection preference, then obtains T-COL17R3 by construction and expression. Through efficacy experiment, it is verified that T-COL17R3 compared with similar products on sale, not only excellent transdermal performance, also has more optimal ability of promoting cell proliferation, migration, anti early glycation product ketone amine and anti late glycation product dicarbonyl compound, better free radical scavenging ability and anti-elastase ability, and can reduce the synthesis of melanin in B16-F10 cell, therefore, T-COL17R3 has excellent application potential in the development of drug composition or skin care product with repair, anti-wrinkle firming or whitening effect.

Embodiments provide for methods of predicting glycation percentage of an amino acid in a therapeutic biomolecule. In one example, a method of predicting a glycation percentage of an amino acid in a biomolecule includes determining a first set of rates for a de-glycation reaction for a first set of temperatures, inferring a second set of one or more rate(s) for the de-glycation reaction for a second set of temperatures, and using the second set of one or more rate(s) to predict the glycation percentage at any temperature corresponding to the second set of temperatures and over any time duration. Also provided are methods for maintaining a glycation percentage of an amino acid within a predetermined glycation percentage range over a shelf-life of a therapeutic biomolecule, and methods for either reducing or increasing a potency of a therapeutic biomolecule in a subject at a time of administration.

Provided is a Bifidobacterium bifidum strain, Bifidobacterium bifidum ibiome001, which can significantly reduce body weight gain and white adipose tissue weight gain of high fat diet-induced obese and diabetic mice, and enhances expression of lipolytic genes ATGL and HSL. Moreover, Bifidobacterium bifidum ibiome001 can significantly lower fasting blood glucose levels, area under curves (AUCs) of the oral glucose tolerance (OGTT) curve, plasma glycated hemoglobin levels and insulin levels of diabetic mice. It is suggested that Bifidobacterium bifidum ibiome001 is a promising potential probiotic strain for the treatment of metabolic syndrome such as obesity, diabetes and the like, which is more effective than other Bifidobacterium strains / compositions.

The invention discloses a method for hydrolyzing and saccharifying wood fiber biomass. The method is characterized by comprising the following steps: (1) washing and drying materials; (2) crushing the materials; (3) preparing a material pretreatment solvent; (4) delignification of the material in a pretreatment solvent; (5) filtering the reaction liquid at normal temperature and normal pressure to separate liquid from solid to obtain cellulose; and (6) adding cellulose and polypeptide into deionized water according to a ratio of 2: 1-1: 2 in a solid-to-liquid ratio of 1: 50-1: 100, adding a NaOH solution to adjust the pH value of the solution to 3-8, and reacting at 40-95 DEG C under the protection of nitrogen for 6-16 hours to obtain the product glucose.

The invention relates to the technical field of biological medicines, in particular to a Chinese wolfberry all-component mixture and application thereof in treatment of diabetes mellitus. The Chinese wolfberry all-component mixture is prepared through the following steps: S1, taking Chinese wolfberry fruits, performing continuous extraction for three times, combining extracting solutions obtained by the three times of extraction, performing filtration, and performing concentration so as to obtain a Chinese wolfberry fruit extract; s2, taking leaf buds, carpopodium and branches of the Chinese wolfberry, performing continuous extraction for three times, combining the three extraction solutions, filtering, and concentrating to obtain a leaf bud / carpopodium / branch extract of the Chinese wolfberry; and S3, mixing the Chinese wolfberry fruit extract obtained in the step S1 with the Chinese wolfberry leaf bud / fruit stem / branch extract obtained in the step S2, filtering, performing graded purification by adopting an ultrafiltration method, intercepting 20-100kDa components, and sterilizing to obtain a Chinese wolfberry all-component mixture. The Chinese wolfberry all-component mixture disclosed by the invention can be used for remarkably reducing a plurality of biochemical index levels such as fasting blood-glucose, glycosylated hemoglobin, triglyceride and extremely low density lipoprotein of a mouse with type 2 diabetes mellitus.

An isolated or purified AON for modifying pre-mRNA splicing in the Receptor for Advanced Glycation End-products (RAGE) to modulate splicing of the RAGE gene transcript or part thereof is provided.

The application discloses a method for producing ethanol by poplar enzymolysis fermentation, and adopts metal chloride and ethanol aqueous solution to pretreat poplar firstly, and then carries out simultaneous saccharification and fermentation to produce ethanol; the metal chloride is aluminum chloride and / or chromium chloride. After the poplar is pretreated by the method, the simultaneous saccharification and fermentation is carried out, when the substrate concentration of the poplar is 30%, the yield of glucose produced by poplar enzymolysis can reach 62.28 g / L; when the substrate concentration of the poplar is 32%, the yield of glucose produced by poplar enzymolysis can reach 64.85 g / L. The method can effectively remove lignin, improve the reaction activity of cellulase, effectively improve the efficiency of poplar enzymolysis fermentation, and improve the yield of ethanol.

The invention relates to the technical field of biology, in particular to pseudomonas fluorescens GS-1 for synthesizing polyhydroxyalkanoate and application of the pseudomonas fluorescens GS-1, the pseudomonas fluorescens GS-1 is preserved in China General Microbiological Culture Collection Center on February 6, 2026, and the preservation number is CGMCC No.37739; the strain carries a phaC gene and can synthesize PHA, and the straw sugar PHA conversion efficiency reaches 87.7% of that of a glucose system; the method has inherent lignocellulose hydrolysate inhibitor tolerance, and the PHA synthesis capability retention rate in the complex self-made corn straw saccharification liquid reaches 91.4%. A high-quality strain resource with direct application potential is provided for producing the PHA based on cheap straw saccharification liquid.

The invention provides a production method for increasing the yield of maltose, which comprises the following steps: proportioning, liquefying and saccharifying, controlling the concentration of starch milk through proportioning, reducing the concentration of syrup, improving the flowability of a system, obtaining a liquefied liquid with a low DE value in the liquefying process, and simultaneously adjusting the pH values of different reaction steps, so that the yield of maltose is increased. Finally, after saccharification is completed, the ultrahigh malt syrup which is low in content of glucose, maltose trisaccharide, maltose trisaccharide or more and high in maltose content is obtained, and the highest maltose content can reach 93%.

The present disclosure relates to a novel peptide and a composition for alleviating skin aging, a composition for caring for skin damage or alleviating skin sagging, a composition for reversing aging, an anti-glycation composition, a composition for preventing hair loss or promoting hair growth, and a composition for improving skin barrier function, wherein the compositions comprise the novel peptide.

The invention relates to the technical field of deep processing of starch, in particular to a method for preparing dietary fiber, which comprises the following steps: (1) mixing starch with acid, and baking to obtain pyrodextrin; (2) performing enzymolysis modification on the pyrodextrin obtained in the step (1) in the presence of starch branching enzyme, and then performing saccharification treatment in the presence of glucamylase to obtain crude resistant dextrin liquid; and (3) mixing the resistant dextrin crude liquid obtained in the step (2) with inulin. According to the preparation method provided by the invention, the content of resistant components in the resistant dextrin crude liquid and the yield of the resistant dextrin can be improved, the preparation time of the resistant dextrin is greatly shortened, and the cost is saved. The prepared dietary fiber can be widely applied to food preparation, such as preparation of health-care products, special dietary foods, beverages, seasonings, candies or milk powder.

The invention provides an N-glycosylation detection method of an antibody drug. The method comprises the following steps: (1) desaccharification, (2) fluorescence labeling, (3) magnetic bead binding purification, and (4) liquid chromatographic analysis. Compared with a traditional method in the prior art, the method has the advantages that the time of an enzyme digestion step is shortened, a drying step before labeling is omitted through improvement of a fluorescence labeling liquid, traditional HILIC purification column separation is replaced by magnetic bead purification separation, the purification and overnight drying steps after labeling are completed in ten minutes, and the method is simple and convenient to operate and high in practicability. And the time and the cost of antibody drug N-glycosylation detection can be saved.

The invention provides a biomarker composition and an application thereof. The biomarker composition comprises the following proteins: MMP12, UMOD, HbA1c, FGF9, NINJ1 and PRSS8. According to the biomarker composition provided by the invention, through combination of matrix metalloproteinase 12, uromodulin, glycosylated hemoglobin, FGF9, NINJ1 and serine protease 8, the problem of lack of a specific marker for aortic wall structure damage in the prior art is solved.

The application relates to a biochemical analyzer and a detection method for detecting glycated hemoglobin. The hemoglobin concentration and the glycated hemoglobin concentration of a to-be-detected whole blood sample are obtained, and the content proportion of the glycated hemoglobin is calculated according to the hemoglobin concentration and the glycated hemoglobin concentration. The hemoglobin concentration is obtained by the following steps: obtaining a first absorbance of a to-be-detected sample liquid at a hemoglobin measurement wavelength, wherein the to-be-detected sample liquid is prepared by mixing a to-be-detected whole blood sample, a hemolytic agent and a hemoglobin detection reagent; obtaining at least two second absorbances of the to-be-detected sample liquid at at least two different correction wavelengths, wherein the correction wavelengths are designed so that the absorbance of a lipid interferent at the correction wavelength is greater than the absorbance of hemoglobin at the correction wavelength; correcting the first absorbance according to the second absorbance; and determining the hemoglobin concentration according to the corrected first absorbance. The application can improve the accuracy of hemoglobin detection.

A method of providing personalized treatment for a diabetes patient including a remote device which is configured to receive a first data indicative of an analyte level of a subject during a first time period, retrieve a first glycated hemoglobin level for the subject associated with the first time period, calculate a first personal apparent glycation ratio for the first time period using the received first data and the retrieved first glycated hemoglobin level, compare the calculated first personal apparent glycation ratio to a representative apparent glycation ratio, generate a recommendation based on the comparison, and display a graphical interface comprising the calculated first personal apparent glycation ratio, the representative apparent glycation ratio, and the comparison.

The present application belongs to the technical field of glucose preparation, and particularly relates to a process for producing glucose by using corn dry method. The process for producing glucose by using corn dry method provided by the present application comprises the steps of corn kernel pretreatment, freezing treatment, high-pressure steam explosion treatment, liquefaction, saccharification, refining and the like. The process for producing glucose by using corn dry method provided by the present application combines freezing and thawing, high-pressure steam explosion and multi-stage enzymolysis and the like process conditions for regulation and control, effectively improves the liquefaction efficiency, enhances the saccharification effect and speeds up the saccharification reaction speed, so that high-purity glucose with stable quality is quickly obtained, which meets the current standard and specification requirements. Moreover, the process can shorten the time required for liquefaction and saccharification, effectively reduce energy consumption and lower the overall production cost, and a more efficient conversion process ensures that more starch is converted into glucose, improves the raw material use efficiency, and maximizes the reduction of resource waste.

The invention discloses a production process of high-purity malt syrup, which comprises the following steps: S1, starch is subjected to size mixing and liquefaction, and the DE value of liquefaction is controlled at 1-2%; s2, controlling the temperature of the liquefied liquid to 55-60 DEG C, controlling the pH value to 4.2-7.2, and adding debranching enzyme and beta amylase for saccharification treatment; s3, after saccharification is completed, ethanol is added until the concentration is 20%, the reaction continues for 2 h, the ethanol concentration is increased to 40%, and the reaction continues for 2 h; s4, cooling to 30-35 DEG C, adding ethanol until the concentration is 80%, carrying out enzyme deactivation, and standing for crystallization; and S5, centrifugally separating the crystal substance, and drying to obtain the maltose. Through the design of multienzyme synergy, step-by-step enzymolysis, ethanol denaturation and the like, the purity and the yield of the maltose product are greatly improved.

The invention discloses haliotis discus hannai I-type collagen peptide as well as a screening method and application thereof, and belongs to the technical field of bioactive peptides. The peptide sequences of the haliotis discus hannai I type collagen peptide are GAAGDK and DSQSAR; the screening method specifically comprises the following steps: (1) virtual enzymolysis of the haliotis discus hannai I-type collagen; (2) screening the bioactive peptide; and (3) molecular docking. According to the invention, through rational design and a virtual screening strategy, a human gastrointestinal tract digestion process is simulated to carry out targeted virtual enzymolysis, a multi-target molecular docking technology is combined, brand-new bifunctional peptides GAAGDK and DSQSAR are accurately predicted and screened from a specific collagen sequence, and aiming at a dual-action mechanism of AGE-RAGE axis and oxidative stress, the application has the advantages of high sensitivity, high sensitivity and high stability. The composition has remarkable synergistic interaction potential in the aspects of preventing diabetic complications and delaying skin and body senescence, and a brand new core raw material is provided for developing next-generation anti-saccharification functional food and skin care products.