141results about How to "Disinhibition effect" patented technology

Described are various compounds, in particular iminosugars, and methods for the treatment of proteostatic diseases, in particular lysosomal storage disorders. The compound may be a pharmacoperone of an enzyme selected from: (a) Acid alpha-glucosidase; (b) Acid beta-glucosidase; (c) glucocerebrosidase; (d) alpha-Galactosidase A; (e) Acid beta-galactosidase; (f) beta-Hexosaminidase A; (g) beta-Hexosaminidase B; (h) Acid sphingomyelinase; (i) Galactocerebrosidase; (j) Acid ceramidase; (k) Arylsulfatase A; (l) alpha-L-Iduronidase; (m) Iduronate-2-sulfatase; (n) Heparan N-sulfatase; (o) alpha-N-Acetylglucosaminidase; (p) Acetyl-CoA: alpha-glucosaminide N-acetyltransferase; (q) N-Acetylglucosamine-6-sulfate sulfatase; (r) N-Acetylgalactosamine-6-sulfate sulfatase; (s) Acid beta-galactosidase; (t) Arylsulfatase B; (u) beta-Glucuronidase; (v) Acid alpha-mannosidase; (w) Acid beta-mannosidase; (x) Acid alpha-L-fucosidase; (y) Sialidase; and (z) alpha-N-acetylgalactosaminidase.

The invention discloses a preparation method of natural spice 2-phenethyl alcohol, which is implemented by selecting Candida drusei, Saccharomyces cerevisiae, Kluyveromyces marxianus, inoculating cultivated seed liquid or active dried yeast powder into fermentation culture medium, taking L-PHE produced by fermentation method or enzyme method as substrate, performing liquid aerobic fermentation in a fermentation shake flask or a fermentation tank, adding solid phase absorption medium, L-PHE and glucose in the process of fermentation, obtaining the solid phase absorption medium in a filtration or centrifugation manner, then adopting an elution solvent to carry out elution on the absorption medium, further distilling the eluent to obtain the natural 2-phenethyl alcohol with purity of 97.5-99.5%. The invention combines the fermentation and absorption separation of 2-phenethyl alcohol, has low production cost and excellent aroma quality of the product.

The present invention belongs to the field of biological sterol transforming technology, and is especially biological sterol transforming process with cyclodextrin and its derivative and its preparation. In a transforming system, cyclodextrin or its derivative in certain amount is added, or substrate and cyclodextrin or its derivative in certain ratio are made to form inclusion compound, so as to facilitate transformation. The process of the present invention is especially suitable for microbial transformation of hydrophobic compound, and can raise the solubility of reaction substrate in water solution, raise the substrate utilizing rate and reaction rate, release substrate and product inhibition and raise product converting rate. When the substrate concentration is 3-20g/L, the present invention has conversion period shorted to 3-4 days, conversion rate of 50-95 % and high industrial application value.

the invention discloses a realizing method of microbe original separating fermenting method through inorganic film in the high-density fermenting domain, which comprises the following parts: ferment tank, inorganic film component, chromatographic column, hollow fiber film component or extracting device, compensating tank, liquid reserving tank and peristaltic pump. the invention can separate object product or side-product from fermenting system, which inhibits microbe seriously.

The invention discloses a method for preparing natural medicines by coupling glycosidase catalysis and salting-out extraction, and belongs to the technical field of bioengineering. The method is characterized in that enzymatic hydrolysis reaction is coupled with salting-out extraction of a product. Glycosidase and the natural product are added into a salting-out extraction system, and the glycosidase is utilized to convert the natural product containing glycosyl into an active ingredient of which the glycosyl is removed; meanwhile, the product and a substrate are separated through the extracting capacity of the system. The invention has the advantages of developing a novel reaction and separation coupled method easy for industrial production, and improving production efficiency.

The invention provides a method for producing antimicrobial peptide through fermentation of brevibacillus laterosporu. In fermentation of the brevibacillus laterosporu, when the yield of the antimicrobial peptide is no longer increased, active carbon is directly added into the fermentation liquor; as the active carbon adsorbs the fermentation product, the inhibiting effect of the product is relieved, and the density of the thallus and the yield of the antimicrobial peptide are greatly improved. According to the method, the active carbon is taken as an adsorption stripping coupling agent, has low cost, is environmentally friendly, and can be directly added in the fermentation process without being activated in advance; the antimicrobial peptide can be adsorbed without regulating the pH value of the fermentation liquor; and the method is simple and easy to promote, and the active carbon can be regenerated through desorption after fermentation, thus being recycled repeatedly, and lowering the production cost.

The present invention discloses an apparatus and a method for butanol production through dual bacteria immobilization anaerobic fermentation, wherein the apparatus comprises a fermentation culture medium storage tank, a butyric acid production immobilization reactor A, a butanol production immobilization reactor C, and a product collection tank, wherein the devices are sequentially connected in series through pipelines. According to the present invention, butyric acid production anaerobic bacteria and butanol production anaerobic bacteria are respectively immobilized in the butyric acid production immobilization reactor A and the butanol production immobilization reactor C to carry out dual bacteria immobilization, and a fermentation culture medium is controlled to flow from the fermentation culture medium storage tank, sequentially flows through the butyric acid production immobilization reactor A and the butanol production immobilization reactor C to carry out continuous anaerobic fermentation, and finally flows into the product collection tank, wherein the butyric acid production anaerobic bacteria adopts a substrate to produce butyric acid, and the butanol production anaerobic bacteria adopts the butyric acid as a substrate to produce butanol. With the method, a fermentation period can be substantially shortened, and substrate conversion rate and yield of the butanol can be increased.

The present invention relates to Serratia sp. A5 and applications thereof. The Serratia sp. A5 is preserved in the China General Microbiological Culture Collection Center on August 28, 2014, and the preservation number is CGMCC No.9621. According to the present invention, the Serratia sp. A5 is isolated from soil, the suitable growth temperature is 25-35 DEG C, the pH value is 7.0-7.5, and the Serratia sp. A5 has the lipase production ability; and the Serratia sp. A5 can be applied for degrading grease, relieving the inhibition effect of the grease in food waste anaerobic fermentation, increasing the biogas yield by 15-30%, and effectively increasing anaerobic fermentation hydrolysis and biogas production efficiency of other waste containing fat and the like or waste water, and further provides important significance for promotion of harmless, resource and energy utilization of organic waste.

The invention relates to a production process for producing gamma-cyclodextrin by a biological method and belongs to the technical field of cyclodextrin production. The production process solves the technical problems to provide the process for producing gamma-cyclodextrin by the biological method, and the production cost of the gamma-cyclodextrin is reduced. The production process has the technical scheme that the gamma-cyclodextrin glucosyltransferase from alkalophilic bacillus is adopted for producing the gamma-cyclodextrin, the starch pulp regulation is carried out according to the concentration being 10 percent, the materials are stirred for 5 to 15 minutes under the condition being 60 to 90 DEG C, the temperature is set to be 40 to 60 DEG C, the pH is regulated to be about 10.0, the gamma-cyclodextrin glucosyltransferase is added according to a quantity of 1 to 10 units for one gram of starch, after 2 to 5 percent (w/v) organic solvents are added, the sufficient reaction is carried out for 8 to 10 hours, the organic solvents are recovered, and the gamma-cyclodextrin is obtained by adopting a crystallization method.

The invention provides a method for eliminating glucose inhibition effect of clostridium acetobutylicum, and a recombinant strain of which the ccpA gene expression of clostridium acetobutylicum is inhibited in a genome. The method for eliminating the glucose inhibition effect of clostridium acetobutylicum is implemented by inhibiting the ccpA gene expression of clostridium acetobutylicum. By using the method provided by the invention, the ccpA gene expression of clostridium acetobutylicum is interrupted. In the strain provided by the invention, after the ccpA gene is subject to insertional inactivation through group II intron, the capability of the strain of synchronously utilizing xylose and glucose is obviously improved, and the concentration of converted ABE is correspondingly increased at the same time. The engineering strain constructed by the invention eliminates the glucose inhibition effect and can synchronously utilize glucose and xylose for fermenting, thus the strain has the potential in acetone-butanol fermentation with hydrolyzate of lignocelluloses.

Disclosed is a transfer factor solution for animals, wherein its preparing process consists of washing freeze chicken spleens and chopping, charging distilled water and comminuting cells to homogenate, freezing eight times at minus 30 deg C, centrifuging the homogenated liquid, separating deposition and filtering supernatant fluid with microporous filtering film, carrying out hyperfiltration with ultrafiltration membrane to obtain stock solution, removing viruses, charging niacinamide as stabilizer, filtering and degerming through microporous filtering film, charging glycerine and split charging directly.

The invention discloses a nanometer montmorillonite feed additive compound for detoxifying and diarrhea prevention of livestock and poultry, and belongs to the field of animal feed additives. The nanometer montmorillonite feed additive compound is prepared from the following components in percentage by mass: 54.0-82.8 percent of nanometer montmorillonite, 10-20 percent of organic zinc, 2-10 percent of xylooligosaccharide, 0.2-1.0 percent of glucose oxidase and 5-15 percent glucose. The nanometer montmorillonite feed additive compound has the advantages that the diarrhea of piglets and chicks can be effectively prevented and treated, the growth property of livestock and poultry is improved, the survival rate of livestock and poultry, especially small livestock and poultry, is obviously improved, and the culture benefit is increased.

The invention relates to a mycotoxin detoxification agent. The mycotoxin detoxification agent comprises the following components of modified aluminosilicate powder, yeast mannan, a microecological preparation and a plant extract. Compared with the prior art, the mycotoxin detoxification agent disclosed by the invention has treble detoxification efficacy of adsorption, degradation and toxin expelling, unification of the broad-spectrum property and the specificity of the mycotoxin detoxification agent can be realized, and the raising benefits can be effectively increased.

The invention provides a method for asymmetrically synthesizing (2S,3R)-p-methylsulfonylphenylserine by one-bacterium multi-enzyme whole cells, which belongs to the field of biocatalysis and enzyme engineering. By simultaneously expressing L-threonine transferase (PsLTTA), ethanol dehydrogenase (ApADH) and formate dehydrogenase (CbFDH) in escherichia coli BL21 (DE3) engineering bacteria, asymmetric efficient biosynthesis of (2S,3R)-p-methylsulfonylphenylserine is realized. The key chiral building block (2S,3R)-p-methylsulfonylphenylserine of the beta-amino alcohol antibiotics can be obtained through one-step reaction at normal temperature and normal pressure, meanwhile, the inhibition effect of a byproduct acetaldehyde on the PsLTTA is relieved, and the method is an efficient green biosynthesis way.

The invention specifically relates to a method for increasing the expression quantity of a Fab antibody in gene engineering, belonging to the technical field of gene engineering. The method comprises the following steps: construction of a recombinant plasmid pVEGF-FAB; preparation of specific protease defective cell mutants (delta pepN, delta DegQ, delta dcp and delta pepP); transformation; inducible expression of the Fab antibody; etc. The method provided by the invention is special and innovative in reconstruction of host cell strains; prepared host cell strains can eliminate inhibition or degradation effect on expressed Fab due to deletion of specific protease, so the expression quantity of Fab antibody can be increased; thus, the method has good application value in expression and preparation of specific Fab antibodies, provides good reference for expression and preparation of other antibody proteins, and thus has good practical value and scientific research significance.

This invention relates to the use of one or more RNA target protectors to inhibit the binding of an RNA, e.g., small RNA, to a target RNA (e.g., a target mRNA), thus increasing the stability of the target RNA and its function (e.g., increasing the gene expression of the gene corresponding to a target mRNA). An RNA target protector may be, for example, an oligonucleotide, e.g., a morpholino, or a small molecule. The invention further relates to the treatment of a human patient in need thereof with one or more RNA target protectors.