562 results about "Microbial transformation" patented technology

The invention discloses a kitchen waste disposal method, which comprises the following steps of: (1) screening and crushing kitchen waste; (2) carrying out damp and hot hydrolytic treatment; (3) carrying out solid-liquid separation; (4) carrying out fat separation; (5) pulping; (6) carrying out fermentation pretreatment, and (7) converting solid matter microorganism. According to the kitchen waste disposal method disclosed by the invention, from the view point of improving the biodegradation efficiency and the biogas production efficiency of the kitchen waste, the methane is prepared from compound microorganism bacterium agent and the processed kitchen waste by mixing and fermenting, and the microorganism bacterium agent fundamentally solves the problems of high saline matter and high fat of the kitchen waste and odour in a biological treatment process, and greatly improves the efficiency of producing the methane by anaerobic digestion.

The present invention belongs to the field of bioengineering technology, and especially a kind of couple producing biological diesel oil and 1, 3-propylene glycol. The present invention features that the course of lipase catalyzed reaction between methanol or ethanol and fat to produce biological diesel oil and the course of biologically converting glycerin into 1, 3-propylene glycol are coupled via membrane filtering for simultaneous proceeding. The present invention has the effects of eliminating the suppression of short-chain alcohol and glycerin on lipase, prolonging the service life of immobilized enzyme, omitting the glycerin separating and extracting step, lowering the production cost, saving time, raising production efficiency and providing economic and feasible process for producing biological diesel oil and 1, 3-propylene glycol.

The invention discloses a disease-resistant growth promoter for aquatic animals and a preparation method thereof. The disease-resistant growth promoter is characterized in that a novel environmentally-friendly feed additive is prepared by combining the traditional Chinese medicine with a modern microbial transformation technology. The preparation method comprises the following steps: mixing dandelion, licorice root, lightyellow sophora root, barberry root and the like after crushing and screening; adding bran, soybean meal, corn flour and water as a fermentation culture medium, regulating thepH value, sterilizing, cooling, adding a strain, and fermenting; and drying after the fermentation is finished. The disease-resistant growth promoter for the aquatic animals is formed by virtue of beneficial combination of the traditional Chinese medicine and probiotics, can be used for replacing antibiotics and has the effects of resisting bacteria, preventing verminosis, resisting virus, improving the immunity, promoting the growth of the aquatic animals and the like; no toxicity, no side effects, no pollution, no residue and no drug resistance are produced during the long-term use of the disease-resistant growth promoter; and the effects are stable and reliable, and the requirements of people on safe production of aquatic products can be met. The disease-resistant growth promoter for the aquatic animals can also be applied as a veterinary drug by improving the using dose.

A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, such as gelatin, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped container and is stored in the liquid storage sink, thereby causing the carrier to make contact with the warm water. The contact with the warm water causes the carrier to be solated, and mixed and diluted with the warm water. Then, the suction pump of the filtration device is activated for suction and filtration of the mixed liquid through the filter. By using the filter having a shape not allowing microbes to pass through and having a gap, only microbes are captured on the filter after suction and filtration. Polymers and dust to be a background upon an ATP measurement pass through the filter by filtration to be cleanly eliminated.

The invention relates to a method for producing protein feed material by utilizing food wastes, belonging to the field of solid waste treatment. The method comprises the following steps: separating and crashing food wastes, and then carrying out heat-moisture treatment; adding bran in solid matters after solid-liquid separation, and inoculating appropriate among of microbes for anaerobe fermentation so as to obtain the protein feed material. Microbes of the solid matters are converted by adding 10-15 percent of bran, taking aspergillus niger, candida utilis and lactobacillus as mixed fungicides according to the ratio of 1: 2: 2 with the inoculation amount of 0.5-2 percent and carrying out anaerobic fermentation for 35-55h at 30 DEG C. The treated product reaches requirements of Feed Hygiene Standard (GB13078-2001) in China, is not easy to go bad and easy to be stored, and bovine-derived and ovine-derived materials of the product are measured to be both negative. The method can effectively kill pathogenic microbes in the food wastes, converts macromolecular proteins, such as animal-based protein, and the like into micromolecular proteins that are absorbed more easily, thereby also reducing the risks caused by isogenous invitro-cannibalism of food feed products.

The invention belongs to the technical field of microelement medical external drug pillows (bags) and relates to an all-element drug pillow (bag) which is rich in selenium and germanium medical stoneand has the functions of cancer prevention and life prolonging. Specifically, saccharomycetes and lucid ganoderma edible fungus are used as carriers, and organic selenium and organic germanium necessary for achieving element balance of human bodies are obtained by adopting a microbiological transformation technology concerning rich-selenium and rich-germanium resources. A bee-series mixture, a traditional Chinese medicine secret recipe and medical stone with a strong capability in absorption and pollution purification are added. After ultralow-temperature crushing with a modern high and new technology, a 60-mesh sieve is used for screening. Then the drug pillow (bag) can be prepared. Due to the drug pillow (bag), a plurality of active components with overall and rich nutrients can be obtained by means of the absorption of the mouth, the nose and the scalp trichopores, and even the selenium and germanium elements which are rare on the earth can be absorbed so as to fully satisfy the element need of the human bodies; therefore, the drug pillow (bag) achieves the aims of supplementing, adjusting and inhibiting elements in the bodies for the sake of element balance, effectively preventing and curing diseases and cancers, building the body and prolonging the life.

The invention relates to a biological carbon modular composite vertical-flow artificial wetland system which comprises a wetland bed body, a water inlet tank, a gravel layer, a modular biological carbon layer, a mixed filler layer, a filtering medium layer, wetland plants, a reflux unit and a water distributor. When the wetland operates, influent water passes through the anoxic gravel layer and biological carbon layer, the aerobic mixed filler layer, and the anoxic biological carbon layer and filtering medium layer. The reflux unit is arranged inside, and pollutants in sewage are removed under the filtration-adsorption-precipitation action, microbial conversion action and plant absorption action. The biological carbon modular composite vertical-flow artificial wetland system has the advantages of blockage prevention, high hydraulic load, favorable nitrogen / phosphorus removal effect, stable operation and the like.

A stable system for producing liquid products such as ethanol, butanol and other chemicals from syngas components contacts CO or a mixture of CO2 and H2 with a hydrophilic membrane under anaerobic conditions and transfers these components into contact with microorganisms contained as a biofilm on the membrane. Maintaining the microorganisms as a biolayer on the surface of the membrane facilitates cleaning of the membrane surface that retains the biofilm. In addition the shell gas space that surrounds the membranes may be flooded to reduce or remove the biofilm. Agitation of the liquid, by for example the bubbling of gas in the surrounding shell space, can fully or partially remove the biofilm from the membrane.

The invention discloses a method for producing a food ingredient rich in nitrite and flavonoid by use of celery leaves, belonging to the field of vegetable deep-processing technology. According to the invention, the celery leaves are used as a raw material which is subjected to sorting and cleaning, decoloring, pulping, microbial conversion, drying and crushing; and a food ingredient product celery powder is produced which contains 1.8% of nitrite, 3.5% of flavonoid and less than or equal to 5% of water. The food ingredient celery powder can be used for processing meat products, and has effects of protecting color, resisting oxidation and eliminating free radicals. If the celery powder prepared by the method disclosed by the invention accounts for 0.75%, the equivalent amount of nitrite and residual amount of nitrite in meat products both meet the requirements of GB2760-2007.

The invention discloses a strain for producing D-piscose by using microorganism to transform D-fructose and a preparation method thereof, pertaining to food biotechnology field. The invention relates to a strain of spherical Rhodobacter sphaeroides SK011 screened from bottom mud of a fish-pond with the preservation number, CCTCC NO: M 207185, and the preparation method that the spherical Rhodobacter sphaeroides SK011 is used for transforming the D-fructose and producing the D-piscose through culture and fermentation. The invention adopts the SK011 as the strain and a fermentation culture medium which consists of carbon and nitrogen sources, inducer and inorganic salt; the obtained strain is further treated, so as to obtain cell biocatalyst; then by using the D-fructose as a substrate to do bio-transformation, the D-piscose is prepared. Under optimum conditions during the fermentation culture, fermenting solution or free cells or permeability cells or frozen dry powder or solidified cells thereof are used for transforming the D-fructose for 0.5 to 4.8 hours, and the content of the D-piscose in the transformation solution is 2 to 50g / L.

A system and method of aerobic wastewater treatment provides large mixing bubbles along with small oxygenating bubbles supplied by diffusers. The mixing bubbles are large enough to move wastewater and generate a mixing current as they rise to the surface. The mixing current mixes the wastewater, bacteria and other microorganisms, and the small oxygenating bubbles from the diffusers to promote the bacteria and other microorganisms' conversion of the pollutants contained in the wastewater. The mixing provided by the large mixing bubbles enhances the efficiency of wastewater aeration provided by the small oxygenating bubbles.

The invention provides a novel aged golden-flower dark green tea processing technic, comprising the steps of taking aged tea of various tea leaves as raw material, carrying out humidification and germicidal treatment, adding Fuzhuan tea golden-flower fungus power to the obtained tea base, and then fermenting and cultivating, and drying after fermenting, thus obtaining the aged golden-flower dark green tea finished product. The novel aged golden-flower dark green tea processing technic provided by the invention, which is an import innovation of tea process technology, takes various aged teas as the raw material, fuses the key process of Fuzhuan tea, 'fungus growing', to reprocess, and obtains the aged golden-flower dark green tea through fermenting and drying; the obtained aged golden-flower dark green tea has the advantages that golden-flower is full, fungus potpourri is thick, taste is mellow and smooth, liquor color is bright, and the Fu tea quality flavor is obvious. The processing technic not only inherits part flavour characteristics of primary tea, simultaneously, the unique fungus potpourri caused by microbe fermenting is fused, a large variety of dark green tea products can be produced according to different raw material; meanwhile, aged tea and 'golden-flower' fungus are combined organically, the effective components of tea are converted through microbes, bioavailability is improved, and health care efficacy of tea is enhanced.

The invention relates to a three-microbe multielement active highly-efficient nutrient solution for animals and a production process thereof, belonging to the field of feedstuffs. The three-microbe multi-fungus active highly-efficient nutrient solution for animals is prepared by compounding a microbe conversion solution, a multielement organic nutrient solution and a microzoon enzyme nutrient solution. The microbe conversion solution is prepared by compounding photosynthetic bacteria, bacillus and lactic acid bacteria. The multielement organic nutrient solution is prepared by compounding five main life nutrition elements, such as selenium, germanium, chromium, zinc and calcium. The microzoon enzyme nutrient solution consists of a nutrient solution of fly, maggot and earthworm cast and is catalyzed by using an 863 nano catalyst. Through the three-microbe multielement active highly-efficient nutrient solution for animals, the bred livestock and poultry have the advantages of strong disease resistance, anti-stress capability, improved immunity, high growth rate, good quality and high deodorization efficiency; and the agricultural products, such as meat, eggs, milk, fresh and the like, are purely natural without residue and rich in high nutrient, and also contain high healthcare elements. The three-microbe multielement active highly-efficient nutrient solution for animals disclosed by the invention is widely suitable for the feedstuff of livestock, poultry and animals.

The invention relates to a method for coupled production of cadaverine by using microbial fermentation and microbial conversion. By use of the gene engineering technology, lysine decarboxylase genes with different sources are cloned in Escherichia coli or corynebacterium glutamicum with high yield of lysine for secretory expression, recombinant stains are fermented, the recombinant strains synthesize lysine at the first stage, lysine decarboxylase genes are induced for secretory expression at the second stage, and the lysine is converted into the cadaverine. The method for coupled production of cadaverine by using microbial fermentation and microbial conversion provides a new way for production of the cadaverine, has enormous economic benefit and social benefit and has broad market development prospect.

The invention provides a method for desulfurization of SO2 waste gas. A basic absorption liquid neutralizes the SO2- laden waste gas to eliminate SO2, discharge the purified gas when the content of SO2 reaches the standard; microbe reduces the desulfurizing absorption tail liquid (containing sulfite group, bisulphate group, or sulfuric group), then microbe oxygenizes the liquid, and the SO2 contained is recovered as a existent of elemental sulfur. The liquid treated by microbe is basic, and it can return directly to the desulfurizing absorption unit and be reused as a basic absorption liquid. And it realizes the aim of eliminating waste and recycling sulfur resource. The method for waste gas treatment is easy to operate and leads to none secondary pollution.

The present invention belongs to the field of biological sterol transforming technology, and is especially biological sterol transforming process with cyclodextrin and its derivative and its preparation. In a transforming system, cyclodextrin or its derivative in certain amount is added, or substrate and cyclodextrin or its derivative in certain ratio are made to form inclusion compound, so as to facilitate transformation. The process of the present invention is especially suitable for microbial transformation of hydrophobic compound, and can raise the solubility of reaction substrate in water solution, raise the substrate utilizing rate and reaction rate, release substrate and product inhibition and raise product converting rate. When the substrate concentration is 3-20g / L, the present invention has conversion period shorted to 3-4 days, conversion rate of 50-95 % and high industrial application value.

The invention belongs to the field of microbial pharmaceutics and pharmaceutical engineering, specifically relates to a production method of prednisone acetate by microbial transformation with Arthrobacter simplex as bacteria strain and hydrocortisone acetate as substrate. The method uses Arthrobacter simplex as bacteria strain and comprises the following steps of: performing primary seed culture, performing second fermentation culture, adding hydrocortisone acetate into the fermentation liquid of Arthrobacter simplex to transform hydrocortisone acetate into prednisone acetate, filtering, and collecting cake to obtain prednisone acetate. The bacteria can be prepared into double liquid phase, broken cells or protoplast, each of which has high transformation ratio. The inventive production method replaces cortisone acetate with hydrocortisone acetate as raw material, and has the advantages of high yield, simple process, good economical and practical performance, and less use of harmful reagents; and is important for steroids production with biotransformation method.

The invention belongs to the field of microbial fermentation and particularly relates to a method for fermentation production of AD and / or ADD by utilizing a high-yield strain. The invention discloses a mycobacterium strain which can realize high-efficient transformation of sterol substances for producing the AD and / or the ADD, the classification name of the mycobacterium strain is Mycobacterium.sp-BK-1, and the mycobacterium strain is collected in China General Microbiological Culture Collection Center with the collection number of CGMCC No. 5707. The invention further discloses the method for producing the AD and / or the ADD by utilizing the strain to transform sterol. The strain disclosed by the invention can completely transform the sterol substances with the concentration of 3.5 percent in a fermentation culture medium to the AD and / or the ADD by relying on an existing process for producing the AD and / or the ADD by microbial transformation of the sterol substances, and the transformation rate is about 70-80 percent, so that the single-time production quantity is increased on the basis of ensuring the single transformation rate.

The invention relates to a method for preparing ocean rhodotorula fermentation feed. The method comprises the preparation of solid fermentation base stock of soybean meal, the mixing of a nutrition replenishing agent and the preparation of a solid fermentation medium. The feed is characterized in that ocean rhodotorula and a lactobacillus strain are inoculated in the solid fermentation medium and are subjected to solid fermentation to obtain the ocean rhodotorula fermentation feed. The method has the advantages: 1. macromolecular protein in the soybean meal is decomposed into small molecular peptide which improves the digestive absorption rate of the protein and further balances the composition of amino acid through microbial transformation; 2. the fermentation feed is replenished with astaxanthin and other biological active substances introduced by the metabolism of rhodotorula glutinis, thereby improving the nutritive value of the feed; 3. lactobacillus in the fermentation feed has positive function on improving a microecological flora of intestinal tracts of bred animal; and 4. the method has low fermentation cost, low fermentation condition and equipment requirement, simple operation of process flow and small investment and is suitable for small-scale production of breeding households and feed processing factories.

The present invention relates to methods, systems and compositions, including genetically modified microorganisms, directed to achieve decreased microbial conversion of 3-hydroxypropionic acid (3-HP) to aldehydes of 3-HP. In various embodiments this is achieved by disruption of particular aldehyde dehydrogenase genes, including multiple gene deletions. Among the specific nucleic acids that are deleted whereby the desired decreased conversion is achieved are aldA, aldB, puuC), and usg of E. coli. Genetically modified microorganisms so modified are adapted to produce 3-HP, such as by approaches described herein.

The microbial conversion process of producing acrylamide by applying membrane technology includes the following steps: microbial thallus culture, preparation of heavy thallus suspension, acrylonitilehydration to produce acrylamide with free thallus as biocatalyst, and separating reaction obtained acrylamide hydrate liquid. The present invention features the microfiltering film purification and washing of thallus in fermented liquid to prepare heavy thallus suspension and the ultrafiltering film separation of acrylamide hydrate liquid from biological impurity. The production process of acrylamide has high production efficiency, high thallus utilization, low biological impurity content in hydrate product, and high quality and purity of acrylamide product.

The invention discloses a method for preparing ginseng rare anti-cancer saponin Compound K from composite bacteria, and belongs to the fields of functional food and pharmacy. According to the method, RB1 or ginseng, American ginseng and panax notoginseng serve as raw materials, 1 to 5 percent of sugar and milk powder are added, preferable saccharomycetes for producing beta-glucosaccharase, lactic acid bacteria and bifidobacterium are adopted, fermentation is conducted at 30 to 37 DEG C for 5 to 14 days, microbial transformation of the compound bacteria is conducted, and the rare ginseng containing saponin C-K is obtained. The obtained fermented ginseng powder and panax notoginseng powder have strong and attractive fragrance and good taste, and the health-care effect and the nutritional value of ginseng and panax notoginseng are greatly improved.

The invention provides a method for desulfurization from a SO2- laden waste gas and for recovery of the sulfur resource. A basic absorption liquid neutralizes the SO2- laden waste gas, discharge the purified gas when the content of SO2 reaches the standard; microbe reduces the desulfurizing absorption tail liquid (containing bisulphate group), and then the liquid is oxygenized with chemical treatment to recover elemental sulfur. The desulfurizing absorption tail liquid treated by microbe is basic, and it can return directly to the desulfurizing absorption unit and be reused as a basic absorption liquid; the chemical oxidant is oxygenized by microbe to revive. The invention realizes the aim of eliminating waste and recycling sulfur resource. And it is easy to operate and leads to none secondary pollution.

The invention provides a preparation method for hydroxylation of 11 alpha of an important intermediate of a steroidal hormone substance, and aims to solve the problems that the conversion rate is low and the environment is polluted when a microorganism is used for converting steroidal C11 alpha for hydroxylation in the prior art. The preparation method comprises the following steps: strain breeding, wherein a strain of ochratoxin or rhizopus nigricans is inoculated to a corresponding seed medium for cultivation; substrate emulsification, wherein a substrate selected from one of 17-alpha hydroxyl progesterone, 4-androstenedione or 16,17-alpha epoxy progesterone is subjected to emulsification treatment under the action of a surfactant; fermented cultivation, wherein the ochratoxin or rhizopus nigricans is inoculated to a fermented medium to be cultivated for a period of time, and then one of emulsion liquids of sterilized 17-alpha hydroxyl progesterone, 4-androstenedione or 16,17-alpha epoxy progesterone is added for performing continuous fermented cultivation; extracting a finished product. The method has the advantages of high conversion rate, little pollution, environmental protection, low pressure and the like.

The invention provides a 3-ketosteroid-delta 1-dehydrogenase gene, cholesterol oxidase coded by the same, an expression vector containing a gene sequence of the 3-sterone-delta 1-dehydrogenase gene, a gene engineering recombinant strain containing the expression vector and a method for preparing 3-sterone-delta 1-dehydrogenase. The 3-sterone-delta 1-dehydrogenase provided by the invention is new 3-sterone-delta 1-dehydrogenase. The recombinant strain provided by the invention can be used for transforming 3-keto steroids, wherein the optimized recombinant strain can also be used for transforming androstane-4-alkenyl-3,17-diketone into boldenone, the expressed target protein is a soluble protein, the bottleneck of membrane protein in the traditional industrial production is broken through, and the recombinant strain has great significance in the industrial production of KSDD (3-ketosteroid -delta 1-dehydrogenase). Steroids are produced through microbial transformation, the production efficiency and the product quality of a steroid medicine production system are improved, and the production cost is reduced.

The invention relates to bacillus subtilis. The bacillus subtilis is bacillus subtilis B7-S and has the CCTCC (China Center For Type Culture Collection) No:M2011162; and the bacillus subtilis is obtained by the induction of a seed culture medium with the ferulic acid concentration of 0.2-2.0g / L. The invention also discloses a method for producing vanillin with ferulic acid biotransformed by the bacillus subtilis. The method is used for producing the vanillin by using a microbiological transformation method using the ferulic acid as a substrate, has the transformation efficiency of 55-63 percent and has the advantages of abundance and easy acquisition of raw materials, low production cost, simple production process, safety, cleanness, no pollution, environmental friendliness, and the like;the natural property of the vanillin is fully maintained; and compared with other biotransformation methods, the method saves the cost by 30 percent, and effectively solves a plurality of defects, such as high raw material toxicity existing in vanillin chemical synthesis, serious pollution to the environment by wastes produced in the production process, single purity and aroma of vanillin, low safety, and the like.

The invention relates to a method for preparing demethyl rapamycin by utilizing bacillus megaterium, including that bacillus megaterium is inoculated to slope culture medium to be cultured and then is inoculated into seed culture medium to be cultured, and then the seed culture solution is inoculated into a transformation culture medium. When the bacillus megaterium propagates in the transformation culture medium, substrate rapamycin is added, the bacillus megaterium carries out transformation on the rapamycin, so as to obtain bacillus megaterium transformed rapamycin transformation solution, and the transformation solution contains 29, 42-O-didemethyl rapamycin, 42-O-demethyl rapamycin and 29-O-demethyl rapamycin and other rapamycin derivates. The invention has the advantage that micro organism is adopted to obtain rapamycin derivate. Pollution to the environment is less, action condition is mild, and no group protection is required, and transformation efficiency of rapamycin derivate can be improved by improving micro organism and condition thereof for transforming rapamycin.

The invention discloses a protopanoxadiol peroxide derivatives as well as a preparation method and an application of the protopanoxadiol peroxide derivatives. The structural formulae of the protopanoxadiol peroxide derivatives disclosed by the invention are shown in formulae I, II and III. In the method, the microbial conversion technology is utilized to modify structure of protopanoxadiol successfully so as to obtain various novel compounds. In-vitro anti-tumor cell test shows that the compounds have better anti-tumor activity, so that the compounds can be used as active components of the anti-tumor drug and have wide application.