79 results about "Microbial transformation" patented technology

The invention provides a method for producing pullulan by aureobasidium pullulans, which is characterized in that a microbial conversion method is adopted for the first time, and pullulan is produced by catalyzing conversion of fructooligosaccharide through an enzyme reaction liquid, and the pullulan is produced by taking an aureobasidium pullulans seed culture solution as a carrier, fructooligosaccharide as a substrate and the enzyme reaction liquid as a catalyst. In a 5.0 L biological reaction container, a mode of one-time substrate addition or multiple feeding of fructooligosaccharide and enzyme reaction liquid is adopted, catalytic reaction is performed for 60-72 hr, 61.43 g / L-71.43 g / L of fructooligosaccharide is consumed together, the highest yield of pullulan is 60.2 g / L-64.26 g / L, and the conversion rate of fructooligosaccharide is up to 87.5%-97.98%. Compared with the existing method for producing the pullulan by a fermentation method, the biotransformation method can solve the problem of insufficient enzyme amount of catalytic reaction depended in the pullulan synthesis process on one hand, and also can solve the problem of reverse regulation of the substrate and the product on pullulan synthesis in the pullulan synthesis process on the other hand; and the problems of low substrate carbon source utilization rate, low substrate conversion rate and the like caused thereby are solved.

The invention relates to the technical field of ginsenoside, aims to solve the problem of low conversion rate of ginsenoside Rg3 prepared by adopting a microbial conversion method in the prior art, and particularly discloses beta-glucosidase and a preparation method thereof, and application of the beta-glucosidase in production of ginsenoside Rg3. The preparation method of the beta-glucosidase comprises the following steps: S1, inserting a beta-glucosidase gene into a pET vector to obtain a recombinant plasmid; s2, inoculating the recombinant plasmids into competent cells, culturing the competent cells in an antibiotic-free culture medium and a resistant culture medium in sequence to form monoclonal antibodies, performing IPTG (isopropyl-beta-d-thiogalactoside) induction culture to form monoclonal strains, and storing the monoclonal strains in glycerol to obtain glycerol bacteria; s3, glycerol bacteria are placed in an LB culture medium to be cultured and then transferred into a fermentation culture medium, the fermentation culture medium is placed in a fermentation tank to be fermented, then centrifugal separation, thallus collection and re-suspension are conducted, beta-glucosidase is obtained and used for decomposing ginseng total saponins to produce ginsenoside Rg3, and the conversion rate of main products can reach 98%.

The invention relates to the technical field of aquaculture and ecological environment management, and discloses a novel pond ecological circulation culture method which comprises the steps that a pond is divided into four vertical functional areas including an upper-layer floating bed planting area, a middle-layer net cage culture area, a bottom-layer benthic purification area and a microbial conversion area, a water circulation system is arranged in a matched mode, the area needed by pond site selection is 3000-4000 m, and the water depth is 3.0-3.5 m. The water quality is automatically controlled through a sensor containing dissolved oxygen and the like, and the biological monitoring module is automatically controlled through a high-definition camera and an AI to acquire data in real time, trigger oxygenation, move the net cage and the like; mixed feeding of'basal feed and insect protein ', cross-season crop rotation of floating bed crops and batch harvesting of benthic organisms are performed daily, equipment is maintained regularly, and ecological circulating breeding is achieved.

The invention belongs to the technical field of microbial fermentation, and relates to bacillus cereus capable of biologically converting limonene, the bacillus cereus is preserved in the China Center for Culture Collection, the classification name is Bacillus cereus GJ-02, the preservation number is CCTCC M 20251438, and the preservation date is June 23, 2025; according to the invention, the bacillus cereus strain capable of biologically converting limonene and having efficient conversion capability is separated and screened, a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention belongs to the technical field of microbial fermentation, and relates to a nitrate reducing pseudomonas capable of biologically converting limonene, which is preserved in the China Center for Culture Collection, the classification name of which is Pseudomonas nitritiducens GJ-01, the preservation number of which is CCTCC M 20251439, and the preservation date of which is June 23, 2025, and the preservation number of which is CCTCC M 20251439. According to the invention, the nitrate reducing pseudomonas strain with efficient conversion capability and capable of biologically converting limonene is separated and screened, so that a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention discloses a biological energy feed fermentation yeast based on enzyme-mineral synergistic activation and a preparation method thereof. According to the scheme, dual functions of mineral activation and microbial conversion are focused, clinoptilolite, sodium bentonite and kaolin are taken as mineral carriers, lignite humic acid is taken as a nutrient synergist, brown sugar, rice bran and bran organic matrixes are matched, and mineral lattice structures are broken through mechanical crushing and biological enzymolysis pretreatment, so that the mineral active substances are obtained. And through a double-stage fermentation process of'aerobic fermentation to activate flora and anaerobic aging to enrich metabolites' of microorganisms, mineral ions, humic acid active ingredients and probiotic metabolites are released to the maximum extent, and finally, the high-quality biological energy feed fermentation yeast with nutrition supply, digestion promotion and immune regulation functions is formed. According to the bioenergy feed prepared by conversion of the fermentation yeast, the feed crude protein improvement rate is greater than or equal to 20%, the crude fiber degradation rate is greater than or equal to 40%, the feed conversion rate is improved by 10-15%, the intestinal disease incidence rate is reduced by 60-70%, and the bioenergy feed is suitable for various livestock and poultry and aquaculture scenes.

The application discloses application of SbWOX gene and SbBBM gene in corn breeding and a corn genetic transformation method, and relates to a bioengineering technology.The application provides a nucleic acid molecule with co-expression of SbWOX protein gene and SbBBM protein gene, and corresponding gene co-expression vectors and microbial transformants.The application also provides application of the co-expression of the SbWOX gene and the SbBBM gene in the corn genetic transformation method.The application obtains corresponding genes and mutant proteins based on the SbWOX gene and the SbBBM gene of sorghum, the two genes are co-expressed in the corn genetic transformation process, even without adding plant growth hormones in the antibiotic screening medium in the tissue culture process, the plant genetic transformation process can be normally completed, and the transformation efficiency and the regeneration efficiency of the corn can be remarkably improved, the transformation rate reaches 10.42%, and the differentiation rate reaches 32.89%.

The utility model belongs to the technical field of sludge treatment, and discloses a device for converting bottom mud of an aquaculture pond by utilizing microorganisms to reduce the content of organic carbon, which comprises a treatment bin, the treatment bin is a circular barrel, a rectangular opening is formed in the upper position of the side face of the treatment bin, and two supporting legs are fixedly installed on the side face of the treatment bin. And a fixed mounting cylinder is arranged at the position, corresponding to the rectangular opening, of the side face of the treatment bin, the section of the mounting cylinder is a rectangular cylinder with a concentric-square-shaped cross section, and a movable filter plate is arranged in the mounting cylinder. According to the sludge treatment device disclosed by the utility model, rapid reaction is carried out under the actions of stirring, aeration and fungicide, the rotating cover is moved after the reaction is carried out for 24-48 hours, the rotating cover is moved to the position of the rectangular opening in the side surface of the treatment bin, the rotating disc is moved after the reaction, the rotating disc extrudes sludge, and water is discharged through the connecting pipe; after the sludge is extruded to the position of a second clamping block, the second clamping block is opened, and a mud cake is taken out, so that the production efficiency is improved.

The present invention belongs to the field of microbial transformation technology, specifically a method for preparing a steroid drug intermediate, comprising the following steps: strain activation; seed liquid culture; fermentation conversion in a fermenter; extraction of conversion products; purification of conversion products; structural confirmation of conversion products. By using androstenedione as the starting material, a strain is selected to ferment and prepare 1,4-androstenedione, and the culture medium selected by strain activation is LB solid culture medium, which is cultured in a constant temperature incubator at 28°C for 24 hours after inoculation; LB liquid culture medium is selected for seed liquid culture, and a single colony on a plate is picked up with an inoculation loop and inoculated and then cultured in a constant temperature shaker at 28°C for 16 hours; then the substrate 4-androstenedione is fermented; an equal volume of ethyl acetate is added to the culture medium for extraction and separation, and it is in a white powder after decolorization and purification, and the selected strain has a short conversion time, and the conversion rate can reach 70% in only 24 hours, realizing the function of rapid conversion and achieving the effect of improving the conversion rate.

The invention belongs to the technical field of medicines, and discloses an arbutin amino acid derivative as well as a preparation method and application thereof. According to the invention, a microbial conversion technology is utilized, the arbutin is taken as a substrate, pyroglutamic acid is introduced to the 28 site of the arbutin in a regioselective manner, and the arbutin-28beta-pyroglutamate with a novel structure is obtained. An in-vitro anti-tumor cell test proves that the compound has remarkable anti-tumor activity and can be used as an active component of an anti-tumor drug. A mouse in-vivo experiment proves that the compound has a remarkable anti-osteoporosis effect, can be used as an active ingredient of an anti-osteoporosis medicine, and has wide application.

Microorganisms and bioprocesses are provided that convert gaseous substrates, such as renewable H2 and waste CO2 producer gas, or syngas into high-protein biomass that may be used directly for human nutrition, or as a nutrient for plants, fungi, or other microorganisms, or as a source of soil carbon, nitrogen, and other mineral nutrients. Renewable H2 used in the processes described herein may be generated by electrolysis using solar or wind power. Producer gas used in the processes described herein may be derived from sources that include gasification of waste feedstock and / or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

The invention discloses a telmisartan glucoside derivative, a microbial conversion preparation method thereof and application of the telmisartan glucoside derivative in preparation of a medicine for preventing or treating osteoporosis. The telmisartan glucoside derivative is characterized in that a telmisartan propyl side chain is connected with a glucosyl group. According to the invention, a microbial conversion technology is utilized, telmisartan is taken as a substrate, and glucosyl is introduced into a telmisartan propyl side chain in a regioselective manner, so that the telmisartan glucoside derivative with a novel structure is obtained. Experiments on the osteoblast proliferation promoting activity and the postmenopausal osteoporosis resisting effect of the telmisartan glucoside derivative by using an in-vitro osteoblast model and an ovariectomized induced osteoporosis mouse model prove that the telmisartan glucoside derivative has relatively good osteoblast proliferation promoting activity and postmenopausal osteoporosis resisting activity; the compound can be used as an active component of a medicine for treating osteoporosis, and has wide application.

The invention discloses a method for synthesizing N-acetyl-L-leucine through microorganisms, and belongs to the field of microbial metabolic engineering.The method comprises the following steps that S1, genes alsS, ilvC and ilvD are recombined to a carrier pBBR1MCS1, and a carrier pDY001 is obtained; s2, recombining the genes leuAfbr, leuB, leuC, leuD and cdNAT to a vector pET28a (+), so as to obtain a vector pDY002; s3, the vectors pDY001 and pDY002 are expressed in microorganisms, and an engineering bacterium GDY1 is obtained; and S4, fermenting the engineering bacterium GDY1 by using monosaccharide and / or glycerol as a substrate to obtain a fermentation product containing N-acetyl-L-leucine. The method disclosed by the invention has the beneficial effects that microorganisms expressing alsS, ilvC, ilvD, leuAfbr, leuB, leuC, leuD and cdNAT genes are fermented by taking monosaccharide and / or glycerol as a substrate to obtain a fermentation product containing N-acetyl-L-leucine, so that microbial conversion from monosaccharide or glycerol to N-acetyl-L-leucine can be realized; and a feasible way is provided for large-scale biosynthesis of the N-acetyl-L-leucine.

The invention provides a C3N5 modified composite catalytic electrode, a coupled microbial fuel cell system constructed by the C3N5 modified composite catalytic electrode and a wastewater treatment method. Inlet water of the system is high-concentration new energy wastewater with COD (Chemical Oxygen Demand) of about 20000 mg / L, and the wastewater flows into the reactor from a water inlet at the bottom of the reactor through a peristaltic pump, passes through a microbial anode chamber, a gravel filling layer and an upper cathode chamber and then flows out. A carbon rod is used as a conducting medium of an anode, and wastewater overflows out of a cathode area for effluent collection. The highest COD removal rate reaches 83%, the highest treatment capacity reaches 6kg COD / (m < 3 >. D), and available electric energy is synchronously generated. According to the method, the capacity of converting wastewater pollutants and generating electricity of microorganisms is utilized, the photoelectric response and catalytic capacity of the nano-catalyst are combined, energy-saving and efficient treatment of the pollutants in the industrial wastewater is achieved, meanwhile, secondary pollution is avoided, and the method is a low-carbon and environment-friendly refractory wastewater treatment method with large technical and economic advantages.

The invention relates to the technical field of algae treatment, and discloses a floating type aquatic crop planting device and method based on algae bloom resource utilization, the floating type aquatic crop planting device comprises a ship body, the ship body is provided with an algae collection and dehydration system, an ultrasonic inactivation system, an algae mud microbial agent mixing system and an ecological restoration system; the algae collection and dehydration system comprises a collection conveying belt and a dehydration device which are connected with the ship body, the ultrasonic inactivation system comprises a multi-band ultrasonic array, the algae mud and microbial agent mixing system comprises a stirring device, a microbial agent storage box and a mixing box, and the ecological restoration system comprises a plant planting device, a mixed mud conveying device and a microorganism putting unit. Through the closed-loop process of algae mud inactivation, microbial conversion and bottom mud injection, the double targets of algae mud recycling and bottom mud remediation are achieved, and the method is suitable for treatment of eutrophicated water bodies such as lakes, river channels and aquaculture ponds.

The application discloses application of Hungatella strain in biological transformation of flavone carbon glycoside. The application provides a method for preparing aglycone or aglycone derivative, and comprises the following steps: taking glycoside or plant material containing glycoside as raw material, and adopting Hungatella strain or culture of Hungatella strain to perform biological transformation, so as to obtain aglycone or aglycone derivative. The method provided by the application is a microbial transformation method, and has the advantages of fast reaction rate, high product conversion rate, mild reaction condition, environmental friendliness, simple transformation process, short cycle, low cost and the like. The application is expected to efficiently hydrolyze carbon glycoside bond. The application has great application and promotion value in the field of production of aglycone or aglycone derivative and downstream products thereof.

The invention discloses a Centella asiatica microbial transformation derivative and a preparation method and application thereof. By using the enzyme system of microorganisms, hydroxyl groups are introduced into the non-chemical reaction active sites 7, 20, 21 and 22 of the parent nucleus of Centella asiatica, so that the 20 and 28 positions form lactones, and two novel Centella asiatica microbial transformation derivatives, 2α, 3β, 7β, 23-tetrahydroxy-20(21)-double bond ursane-28β-carboxylic acid and 2α, 3β, 7β, 23-tetrahydroxy-21(22)-double bond ursane-20β, 28β-lactone, are obtained, which can be used as pharmaceutical intermediates. It is confirmed by in vitro anti-tumor cell tests that the two Centella asiatica microbial transformation derivatives have significant anti-tumor activity.

This invention provides an oleic acid hydratase OhyA1 mutant, its preparation method, and its applications, belonging to the fields of biocatalysis and biochemical engineering. The amino acid sequence of the oleic acid hydratase OhyA1 mutant provided by this invention is shown in SEQ ID No. 1. Compared with the parent enzyme, the OhyA1 mutant oleic acid hydratase of this invention exhibits a restructured active site due to the deletion of phenylalanine side chain benzene ring groups at two sites, leading to an increased substrate binding pocket volume and significantly improved catalytic efficiency for long-chain fatty acid substrates containing multiple double bonds (especially linoleic acid). The OhyA1 mutant oleic acid hydratase provided by this invention exhibits high specificity for oleic acid, catalyzing only the cis-9 double bond hydration reaction of oleic acid. The product is a single 10-hydroxy fatty acid, avoiding isomer formation. The product purity can reach over 95%, and the oleic acid conversion rate can reach over 85%, significantly superior to existing microbial transformation systems.

The invention discloses a design and construction method and application of a De novo biosynthesis route of a key intermediate of didrogesterone, and belongs to the technical field of synthesis of key intermediates of didrogesterone. According to the invention, a recombinant S.cerevisiae strain ScBL6 capable of synthesizing 7-DHC from the beginning is constructed, and when the recombinant S.cerevisiae strain ScBL6 is used for fermentation in a 500mL shake flask, the total yield of 7-DHC reaches 214mg / L, and compared with a contrast ScBL2 strain, the total yield of 7-DHC is increased by 15.24 times. In a 5L fermentation tank, the yield of 7-DHC reaches 1.06 g / L, thalli in the tank are crushed, extracted and then concentrated to 20 g / L. Finally, by utilizing the side chain degradation capacity of mycobacterium neoaurum, after microbial conversion is performed for 72 hours, the molar conversion rate of the target product 6-dehydrodidowncohol reaches 78.54%, the accumulated concentration of the product is 15.71 g / L, the molar conversion rate of the 6-dehydrodidowncohol is detected to be 83.49% in 144 hours, and the yield of 7-DHC in the fermentation tank is detected to be 83.49% in 144 hours. And the concentration of the final product reaches 16.72 g / L.

The present invention discloses a method for synthesizing cinnamyl cinnamate from microorganisms, which belongs to the field of microbial metabolic engineering and comprises the following steps: S1, converting aroG fbr gene and pheA fbr The gene is recombined into the vector pBBR1MCS1 to obtain the vector pDY001; S2, the PA2 gene, the osPMT gene and the hcCNL gene are recombined into the vector pET28a(+) to obtain the vector pDY002; S3, the CAR gene and the Sfp gene are recombined into the vector pET28b-pSC101 to obtain the vector pDY003; S4, the vectors pDY001, pDY002 and pDY003 are expressed in microorganisms to obtain the engineered bacteria GDY1; S5, the engineered bacteria GDY1 is fermented with monosaccharides and / or glycerol as substrates to obtain a fermentation product containing cinnamyl cinnamate. The beneficial effect of the present invention is that the expression of aroG fbr pheA fbr Microorganisms expressing the PA2, hcCNL, osPMT, CAR and Sfp genes ferment monosaccharides and / or glycerol as substrates to obtain fermentation products containing cinnamic cinnamate, which can realize the microbial conversion of monosaccharides or glycerol to cinnamic cinnamate, providing a feasible path for large-scale biosynthesis of cinnamic cinnamate.

The invention provides high-selenium-rich pediococcus acidilactici and application thereof. The pediococcus acidilactici provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the pediococcus acidilactici in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.33566. The pediococcus acidilactici provided by the invention is used as a selenium-rich carrier, can convert an inorganic selenium element into organic selenium which can be easily absorbed and utilized by a passive object through microbial conversion, is enriched in pediococcus acidilactici cells, and can be used as a feed additive for breeding animals.

The invention relates to the technical field of biology, in particular to a phenylpyruvate decarboxylase ARO10 protein mutant and application of the phenylpyruvate decarboxylase ARO10 protein mutant in preparation of phenethyl alcohol. The invention discloses an ARO10 mutant. The phenylpyruvate decarboxylase mutant is obtained by mutating one or more of the 335th amino acid, the 339th amino acid and the 628th amino acid of phenylpyruvate decarboxylase ARO10 (the amino acid sequence shown as SEQ ID NO.1) from yeast. Experiments prove that the mutated phenylpyruvate decarboxylase protein can improve the specific reaction on phenylpyruvic acid, so that the phenethyl alcohol generation capability of saccharomyces cerevisiae is improved. Glycosyl transferase ARO10 mutant protein is expressed in microorganisms, the phenethyl alcohol conversion capacity of the microorganisms is obviously enhanced, and the industrial application prospect is good.

The invention provides a method for preparing isobutanol by converting methane through microorganisms and application of the method. The method comprises the following steps: mixing lactococcus lactis and methanophilic bacteria which reach a stable growth period according to a volume ratio of (5-30): 100, and then converting methane to prepare the isobutanol, the conditions for preparing the isobutanol by converting the methane through the microorganisms are as follows: the methane concentration is 10-30%, the conversion temperature is 25-35 DEG C, and the conversion period is 2-7 days. The invention provides a method for catalyzing conversion of methane into isobutanol through mixed culture of two microorganisms, and a channel for preparing isobutanol by using methane as a raw material is opened in a simple mode.

The invention belongs to the field of bioengineering pharmacy, and particularly relates to a method for preparing high-standard exemestane through microbial conversion. According to the method, Nocardia simplex Noc (PN)-TS001 is adopted as a fermentation strain, methylenide is efficiently converted by microorganisms to prepare high-standard exemestane, and through optimization and combination of processes of all stages such as methylenide pretreatment, microorganism fermentation conversion and extraction refining, the substrate feeding concentration is increased to 1%-8%, the conversion rate reaches 88%-95%, the total yield reaches 88%-90%, and the yield of exemestane is increased to 90%. The detection content of the prepared exemestane refined product is more than 99.5%, the impurity content is less than 0.1%, and each index meets the USP standard. The method is simple and feasible in process, environment-friendly, low in cost, high in conversion efficiency, high in yield and high in product quality, and has a very good industrial application prospect.

The present invention relates to a method for the synthesis of isorhamnetin metabolite from the quercetin molecule and the isorhamnetin metabolite synthesized by this method. In this method, isorhamnetin is synthesised from quercetin molecule by microbial transformation of Aspergillus flavus. The method of the present invention provides the synthesis of isorhamnetin with 18.20% yield in a short time and at low cost.

The invention relates to a preparation method of medicinal and edible nutrient freeze-dried powder for enhancing macrophage activity. The preparation method comprises the following steps: S1, inoculating lactobacillus plantarum on a weakly selective solid culture medium for culturing; s2, selecting monoclonal lactobacillus plantarum, inoculating the monoclonal lactobacillus plantarum to a lactobacillus plantarum fermentation culture medium, and culturing; s3, when the concentration of the lactobacillus plantarum reaches the optical density value of 0.5, adding rhizoma polygonati powder, lucid ganoderma powder and mulberry leaf powder into the bacterial liquid for conversion; s4, filtering residues of the rhizoma polygonati powder, the lucid ganoderma powder and the mulberry leaf powder by using filter cloth to obtain a compound nutrient clear liquid; and S5, adding a freeze-drying protective agent into the compound nutrient clear liquid, uniformly stirring, and freeze-drying to constant weight to obtain the compound nutrient freeze-dried powder. Through the design, the utilization rate of active ingredients of the medicinal and edible raw materials can be improved by combining microbial conversion with a freeze-drying technology, and the efficient regulation of the activity of macrophages is realized.

The invention provides a skin anti-aging and firming composition, oil cream and a preparation method thereof, and belongs to the field of medical, dental or cosmetic preparations. The composition comprises a palmaria palmata and inula flower extract, a vaccinium macrocarpum fruit extract, a medicine root extract and the palmaria palmata and inula flower extract, palmaria palmata and inula flower serve as fermentation substrates, aerobic fermentation is conducted through aspergillus niger, and anaerobic fermentation is conducted through pediococcus pentosaceus; and purifying to obtain the product. According to the palmaria palmata inula flower fermentation extract provided by the invention, through sequential fermentation of aspergillus niger and pediococcus pentosaceus, deep biotransformation of a plant matrix is realized. The extract has the beneficial effects that the moisturizing and repairing characteristics of palmaria palmata and the anti-inflammatory and anti-oxidation characteristics of inula flower are fused, new active matters such as lactic acid and small molecule peptide are generated through microbial conversion, and the comprehensive effect is obviously superior to that of a traditional extract; macromolecules are converted into micromolecules easier to absorb through fermentation, bioavailability is improved, the pH value of the product is close to the skin, and the product is mild and low in irritation.

The invention relates to an application method of a special microbial agent for improving the nitrogen fixation efficiency of soybean root nodules, and belongs to the field of agricultural microorganism application. A core strategy of staged time sequence application is adopted: firstly, before sowing or during sowing, a first complex microbial inoculant containing proline secreting bacteria and indigenous infectious microbe inhibiting bacteria is applied into soil, and a pre-culture period is performed for 5-15 days; during the period, proline is converted into indole-3-acetic acid (IAA) by a fourth microorganism which synchronously exists or is introduced, so that a growth-promoting microenvironment is established at the rhizosphere, and meanwhile, a third microorganism inhibits harmful infectious microbes; next, bradyrhizobium or sinorhizobium is applied to the seedlings or rhizosphere. According to the time sequence design, an optimized colonization ecological niche with low competition and high chemotactic signals is created for target rhizobium through a synergistic mechanism of firstly improving the environment and then introducing strains. According to the method, the infection success rate of rhizobium can be remarkably increased, the number of root nodules and nitrogenase activity are increased, soybean root development is synchronously promoted, and the soybean symbiotic nitrogen fixation efficiency and yield are improved.

This invention belongs to the field of pharmaceutical technology and discloses arbutin, its polyhydroxy derivatives, their compositions, and uses. Using a microbial transformation method, this invention successfully modified the structure of arbutin, obtaining three novel arbutin derivatives with polyhydroxy substitutions. In vitro neuronal inflammation assays and neuronal oxidative damage protection assays confirmed that these compounds possess good anti-neuroinflammatory and neuronal damage protective activities. Simultaneously, in vivo Alzheimer's disease mouse model experiments demonstrated that these compounds can significantly improve the learning and memory abilities of AD mice, and can serve as active ingredients in drugs for treating neurological diseases. In vitro antitumor cell assays confirmed that compounds I-III possess significant antitumor activity and can serve as active ingredients in antitumor drugs, with broad applications.

Microorganisms and bioprocesses are provided that convert gaseous substrates, such as renewable H2 and waste CO2 producer gas, or syngas into high-protein biomass that may be used directly for human nutrition, or as a nutrient for plants, fungi, or other microorganisms, or as a source of soil carbon, nitrogen, and other mineral nutrients. Renewable H2 used in the processes described herein may be generated by electrolysis using solar or wind power. Producer gas used in the processes described herein may be derived from sources that include gasification of waste feedstock and / or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.