41 results about "Microbial transformation" patented technology

The invention relates to the technical field of aquaculture and ecological environment management, and discloses a novel pond ecological circulation culture method which comprises the steps that a pond is divided into four vertical functional areas including an upper-layer floating bed planting area, a middle-layer net cage culture area, a bottom-layer benthic purification area and a microbial conversion area, a water circulation system is arranged in a matched mode, the area needed by pond site selection is 3000-4000 m, and the water depth is 3.0-3.5 m. The water quality is automatically controlled through a sensor containing dissolved oxygen and the like, and the biological monitoring module is automatically controlled through a high-definition camera and an AI to acquire data in real time, trigger oxygenation, move the net cage and the like; mixed feeding of'basal feed and insect protein ', cross-season crop rotation of floating bed crops and batch harvesting of benthic organisms are performed daily, equipment is maintained regularly, and ecological circulating breeding is achieved.

The invention belongs to the technical field of microbial fermentation, and relates to bacillus cereus capable of biologically converting limonene, the bacillus cereus is preserved in the China Center for Culture Collection, the classification name is Bacillus cereus GJ-02, the preservation number is CCTCC M 20251438, and the preservation date is June 23, 2025; according to the invention, the bacillus cereus strain capable of biologically converting limonene and having efficient conversion capability is separated and screened, a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention belongs to the technical field of microbial fermentation, and relates to a nitrate reducing pseudomonas capable of biologically converting limonene, which is preserved in the China Center for Culture Collection, the classification name of which is Pseudomonas nitritiducens GJ-01, the preservation number of which is CCTCC M 20251439, and the preservation date of which is June 23, 2025, and the preservation number of which is CCTCC M 20251439. According to the invention, the nitrate reducing pseudomonas strain with efficient conversion capability and capable of biologically converting limonene is separated and screened, so that a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention discloses a biological energy feed fermentation yeast based on enzyme-mineral synergistic activation and a preparation method thereof. According to the scheme, dual functions of mineral activation and microbial conversion are focused, clinoptilolite, sodium bentonite and kaolin are taken as mineral carriers, lignite humic acid is taken as a nutrient synergist, brown sugar, rice bran and bran organic matrixes are matched, and mineral lattice structures are broken through mechanical crushing and biological enzymolysis pretreatment, so that the mineral active substances are obtained. And through a double-stage fermentation process of'aerobic fermentation to activate flora and anaerobic aging to enrich metabolites' of microorganisms, mineral ions, humic acid active ingredients and probiotic metabolites are released to the maximum extent, and finally, the high-quality biological energy feed fermentation yeast with nutrition supply, digestion promotion and immune regulation functions is formed. According to the bioenergy feed prepared by conversion of the fermentation yeast, the feed crude protein improvement rate is greater than or equal to 20%, the crude fiber degradation rate is greater than or equal to 40%, the feed conversion rate is improved by 10-15%, the intestinal disease incidence rate is reduced by 60-70%, and the bioenergy feed is suitable for various livestock and poultry and aquaculture scenes.

The application discloses application of SbWOX gene and SbBBM gene in corn breeding and a corn genetic transformation method, and relates to a bioengineering technology.The application provides a nucleic acid molecule with co-expression of SbWOX protein gene and SbBBM protein gene, and corresponding gene co-expression vectors and microbial transformants.The application also provides application of the co-expression of the SbWOX gene and the SbBBM gene in the corn genetic transformation method.The application obtains corresponding genes and mutant proteins based on the SbWOX gene and the SbBBM gene of sorghum, the two genes are co-expressed in the corn genetic transformation process, even without adding plant growth hormones in the antibiotic screening medium in the tissue culture process, the plant genetic transformation process can be normally completed, and the transformation efficiency and the regeneration efficiency of the corn can be remarkably improved, the transformation rate reaches 10.42%, and the differentiation rate reaches 32.89%.

The utility model belongs to the technical field of sludge treatment, and discloses a device for converting bottom mud of an aquaculture pond by utilizing microorganisms to reduce the content of organic carbon, which comprises a treatment bin, the treatment bin is a circular barrel, a rectangular opening is formed in the upper position of the side face of the treatment bin, and two supporting legs are fixedly installed on the side face of the treatment bin. And a fixed mounting cylinder is arranged at the position, corresponding to the rectangular opening, of the side face of the treatment bin, the section of the mounting cylinder is a rectangular cylinder with a concentric-square-shaped cross section, and a movable filter plate is arranged in the mounting cylinder. According to the sludge treatment device disclosed by the utility model, rapid reaction is carried out under the actions of stirring, aeration and fungicide, the rotating cover is moved after the reaction is carried out for 24-48 hours, the rotating cover is moved to the position of the rectangular opening in the side surface of the treatment bin, the rotating disc is moved after the reaction, the rotating disc extrudes sludge, and water is discharged through the connecting pipe; after the sludge is extruded to the position of a second clamping block, the second clamping block is opened, and a mud cake is taken out, so that the production efficiency is improved.

The invention discloses a telmisartan glucoside derivative, a microbial conversion preparation method thereof and application of the telmisartan glucoside derivative in preparation of a medicine for preventing or treating osteoporosis. The telmisartan glucoside derivative is characterized in that a telmisartan propyl side chain is connected with a glucosyl group. According to the invention, a microbial conversion technology is utilized, telmisartan is taken as a substrate, and glucosyl is introduced into a telmisartan propyl side chain in a regioselective manner, so that the telmisartan glucoside derivative with a novel structure is obtained. Experiments on the osteoblast proliferation promoting activity and the postmenopausal osteoporosis resisting effect of the telmisartan glucoside derivative by using an in-vitro osteoblast model and an ovariectomized induced osteoporosis mouse model prove that the telmisartan glucoside derivative has relatively good osteoblast proliferation promoting activity and postmenopausal osteoporosis resisting activity; the compound can be used as an active component of a medicine for treating osteoporosis, and has wide application.

The invention provides a C3N5 modified composite catalytic electrode, a coupled microbial fuel cell system constructed by the C3N5 modified composite catalytic electrode and a wastewater treatment method. Inlet water of the system is high-concentration new energy wastewater with COD (Chemical Oxygen Demand) of about 20000 mg / L, and the wastewater flows into the reactor from a water inlet at the bottom of the reactor through a peristaltic pump, passes through a microbial anode chamber, a gravel filling layer and an upper cathode chamber and then flows out. A carbon rod is used as a conducting medium of an anode, and wastewater overflows out of a cathode area for effluent collection. The highest COD removal rate reaches 83%, the highest treatment capacity reaches 6kg COD / (m < 3 >. D), and available electric energy is synchronously generated. According to the method, the capacity of converting wastewater pollutants and generating electricity of microorganisms is utilized, the photoelectric response and catalytic capacity of the nano-catalyst are combined, energy-saving and efficient treatment of the pollutants in the industrial wastewater is achieved, meanwhile, secondary pollution is avoided, and the method is a low-carbon and environment-friendly refractory wastewater treatment method with large technical and economic advantages.

The application discloses application of Hungatella strain in biological transformation of flavone carbon glycoside. The application provides a method for preparing aglycone or aglycone derivative, and comprises the following steps: taking glycoside or plant material containing glycoside as raw material, and adopting Hungatella strain or culture of Hungatella strain to perform biological transformation, so as to obtain aglycone or aglycone derivative. The method provided by the application is a microbial transformation method, and has the advantages of fast reaction rate, high product conversion rate, mild reaction condition, environmental friendliness, simple transformation process, short cycle, low cost and the like. The application is expected to efficiently hydrolyze carbon glycoside bond. The application has great application and promotion value in the field of production of aglycone or aglycone derivative and downstream products thereof.

This invention provides an oleic acid hydratase OhyA1 mutant, its preparation method, and its applications, belonging to the fields of biocatalysis and biochemical engineering. The amino acid sequence of the oleic acid hydratase OhyA1 mutant provided by this invention is shown in SEQ ID No. 1. Compared with the parent enzyme, the OhyA1 mutant oleic acid hydratase of this invention exhibits a restructured active site due to the deletion of phenylalanine side chain benzene ring groups at two sites, leading to an increased substrate binding pocket volume and significantly improved catalytic efficiency for long-chain fatty acid substrates containing multiple double bonds (especially linoleic acid). The OhyA1 mutant oleic acid hydratase provided by this invention exhibits high specificity for oleic acid, catalyzing only the cis-9 double bond hydration reaction of oleic acid. The product is a single 10-hydroxy fatty acid, avoiding isomer formation. The product purity can reach over 95%, and the oleic acid conversion rate can reach over 85%, significantly superior to existing microbial transformation systems.

The invention relates to the technical field of biology, in particular to a phenylpyruvate decarboxylase ARO10 protein mutant and application of the phenylpyruvate decarboxylase ARO10 protein mutant in preparation of phenethyl alcohol. The invention discloses an ARO10 mutant. The phenylpyruvate decarboxylase mutant is obtained by mutating one or more of the 335th amino acid, the 339th amino acid and the 628th amino acid of phenylpyruvate decarboxylase ARO10 (the amino acid sequence shown as SEQ ID NO.1) from yeast. Experiments prove that the mutated phenylpyruvate decarboxylase protein can improve the specific reaction on phenylpyruvic acid, so that the phenethyl alcohol generation capability of saccharomyces cerevisiae is improved. Glycosyl transferase ARO10 mutant protein is expressed in microorganisms, the phenethyl alcohol conversion capacity of the microorganisms is obviously enhanced, and the industrial application prospect is good.

The invention belongs to the field of bioengineering pharmacy, and particularly relates to a method for preparing high-standard exemestane through microbial conversion. According to the method, Nocardia simplex Noc (PN)-TS001 is adopted as a fermentation strain, methylenide is efficiently converted by microorganisms to prepare high-standard exemestane, and through optimization and combination of processes of all stages such as methylenide pretreatment, microorganism fermentation conversion and extraction refining, the substrate feeding concentration is increased to 1%-8%, the conversion rate reaches 88%-95%, the total yield reaches 88%-90%, and the yield of exemestane is increased to 90%. The detection content of the prepared exemestane refined product is more than 99.5%, the impurity content is less than 0.1%, and each index meets the USP standard. The method is simple and feasible in process, environment-friendly, low in cost, high in conversion efficiency, high in yield and high in product quality, and has a very good industrial application prospect.

The invention relates to a preparation method of medicinal and edible nutrient freeze-dried powder for enhancing macrophage activity. The preparation method comprises the following steps: S1, inoculating lactobacillus plantarum on a weakly selective solid culture medium for culturing; s2, selecting monoclonal lactobacillus plantarum, inoculating the monoclonal lactobacillus plantarum to a lactobacillus plantarum fermentation culture medium, and culturing; s3, when the concentration of the lactobacillus plantarum reaches the optical density value of 0.5, adding rhizoma polygonati powder, lucid ganoderma powder and mulberry leaf powder into the bacterial liquid for conversion; s4, filtering residues of the rhizoma polygonati powder, the lucid ganoderma powder and the mulberry leaf powder by using filter cloth to obtain a compound nutrient clear liquid; and S5, adding a freeze-drying protective agent into the compound nutrient clear liquid, uniformly stirring, and freeze-drying to constant weight to obtain the compound nutrient freeze-dried powder. Through the design, the utilization rate of active ingredients of the medicinal and edible raw materials can be improved by combining microbial conversion with a freeze-drying technology, and the efficient regulation of the activity of macrophages is realized.

The invention provides a skin anti-aging and firming composition, oil cream and a preparation method thereof, and belongs to the field of medical, dental or cosmetic preparations. The composition comprises a palmaria palmata and inula flower extract, a vaccinium macrocarpum fruit extract, a medicine root extract and the palmaria palmata and inula flower extract, palmaria palmata and inula flower serve as fermentation substrates, aerobic fermentation is conducted through aspergillus niger, and anaerobic fermentation is conducted through pediococcus pentosaceus; and purifying to obtain the product. According to the palmaria palmata inula flower fermentation extract provided by the invention, through sequential fermentation of aspergillus niger and pediococcus pentosaceus, deep biotransformation of a plant matrix is realized. The extract has the beneficial effects that the moisturizing and repairing characteristics of palmaria palmata and the anti-inflammatory and anti-oxidation characteristics of inula flower are fused, new active matters such as lactic acid and small molecule peptide are generated through microbial conversion, and the comprehensive effect is obviously superior to that of a traditional extract; macromolecules are converted into micromolecules easier to absorb through fermentation, bioavailability is improved, the pH value of the product is close to the skin, and the product is mild and low in irritation.

The invention relates to an application method of a special microbial agent for improving the nitrogen fixation efficiency of soybean root nodules, and belongs to the field of agricultural microorganism application. A core strategy of staged time sequence application is adopted: firstly, before sowing or during sowing, a first complex microbial inoculant containing proline secreting bacteria and indigenous infectious microbe inhibiting bacteria is applied into soil, and a pre-culture period is performed for 5-15 days; during the period, proline is converted into indole-3-acetic acid (IAA) by a fourth microorganism which synchronously exists or is introduced, so that a growth-promoting microenvironment is established at the rhizosphere, and meanwhile, a third microorganism inhibits harmful infectious microbes; next, bradyrhizobium or sinorhizobium is applied to the seedlings or rhizosphere. According to the time sequence design, an optimized colonization ecological niche with low competition and high chemotactic signals is created for target rhizobium through a synergistic mechanism of firstly improving the environment and then introducing strains. According to the method, the infection success rate of rhizobium can be remarkably increased, the number of root nodules and nitrogenase activity are increased, soybean root development is synchronously promoted, and the soybean symbiotic nitrogen fixation efficiency and yield are improved.

This invention belongs to the field of pharmaceutical technology and discloses arbutin, its polyhydroxy derivatives, their compositions, and uses. Using a microbial transformation method, this invention successfully modified the structure of arbutin, obtaining three novel arbutin derivatives with polyhydroxy substitutions. In vitro neuronal inflammation assays and neuronal oxidative damage protection assays confirmed that these compounds possess good anti-neuroinflammatory and neuronal damage protective activities. Simultaneously, in vivo Alzheimer's disease mouse model experiments demonstrated that these compounds can significantly improve the learning and memory abilities of AD mice, and can serve as active ingredients in drugs for treating neurological diseases. In vitro antitumor cell assays confirmed that compounds I-III possess significant antitumor activity and can serve as active ingredients in antitumor drugs, with broad applications.

The wine brewing wastewater conversion system provided by the utility model comprises a closed reaction zone, a filtering device and a liquid circulation system. The closed reaction zone receives wine brewing wastewater and corresponding reactants and active matters, the pH value of the wine brewing wastewater is adjusted to a proper interval, organic matters in the wine brewing wastewater are converted by using the active matters, and then the converted clear liquid is fed into the reaction zone by circularly supplementing the reactants and the active matters in an overflowing and filtering manner. Through uninterrupted circulating conversion, muddy water and sediment which are rich in carbon and nitrogen elements and can be converted by a conventional high-concentration organic biochemical treatment mode are obtained, and harmless conversion is achieved by performing conventional high-concentration organic biochemical treatment on the muddy water and sediment. The pH of the reaction bin is adjusted through hydrogen peroxide and ozone, the microbial conversion efficiency can be improved, the conversion problem of the wine brewing wastewater is solved, sludge bulking is controlled through a liquid circulation system, COD parameters of effluent are reduced, and harmless conversion is achieved.

The invention provides arbutin-28beta-pyroglutamyl ester. The arbutin-28beta-pyroglutamyl ester is prepared by adopting a two-stage liquid fermentation process to culture a Circinella muscae CGMCC (China General Microbiological Culture Collection Center) 3.2695 strain. The arbutin-28beta-pyroglutamyl ester is a natural triterpenoid metabolite synthesized through microbial conversion for the first time, has good activity of promoting the growth of motor neurons, and can be used for preparing drugs for treating nervous system diseases.

The invention relates to the technical field of strain screening, in particular to a method for rapidly screening isoliquiritigenin transformed strains on a large scale on the basis of MTHM, a microplate reader is adopted for rapid screening, a transformed product is verified through thin-layer chromatography, high performance liquid chromatography and liquid mass spectrometry, the detection precision is amplified step by step, and the isoliquiritigenin transformed strains can be obtained. The screening efficiency of the isoliquiritigenin transforming bacteria is obviously improved. The method not only is efficient and reliable, but also provides a new technical thought and theoretical basis for development of natural products and application of microbial conversion.

The invention relates to the technical field of feed additive preparation, and discloses a fermentation process for preparing an organic chelated calcium feed additive from inorganic calcium by a microbial conversion method, and the process comprises the following steps: mixing and crushing an inorganic calcium source, a carbon and nitrogen source feed and auxiliary materials to prepare a solid fermentation base material; adding activated composite transformed strain inoculation liquid and adjusting the total moisture content; carrying out aerobic fermentation on the material, and carrying out coordination chelation reaction on organic acid, amino acid and small molecule peptide generated by microbial metabolism and dissociated inorganic calcium to convert into water-soluble organic calcium; and after fermentation, drying with low-temperature hot air, and crushing to obtain an organic calcium product. According to the method, a multi-strain synergistic fermentation system is adopted, acid production, substrate enzymolysis and calcium ion chelation are synchronously completed in the same environment, the conversion efficiency of insoluble inorganic calcium is improved, chemical reagent residues are avoided, and the activity of probiotics and biological metabolic enzymes in the system is effectively reserved.

Microphysiological platforms are configured for measuring kinetic rates relating to drug absorption. These kinetic rates include GI region-specific kinetic rates of drug absorption. The kinetic rates include bioavailability of the drug over time in various tissues. The kinetic rates include drug permeability in various tissues. The kinetic rates include measurements of microbial transformations that occur. The kinetic rates include measured effects of gastric emptying and transit times. The kinetic rates include measurements of fasting and fed states for various tissues.

The application discloses a healthy brewing process of Maotai-flavor liquor based on microbial flora domestication technology and belongs to the technical field of liquor brewing. The method comprises the following steps: in the grain moistening process of the lower layer and the layer forming process, raw materials are subjected to graded acidification pretreatment by adopting a two-stage adding mode of lactic acid and citric acid, so as to promote the release of bound flavor precursors in the kaffir corn cortex; microbial samples are collected from the Maotai-flavor liquor brewing environment, and after enrichment culture, the samples are subjected to three-stage continuous subculture domestication in a culture medium containing gradient concentrations of carbon sources, gradient concentrations of ethanol, gradient concentrations of organic acids and gradient temperatures, so as to obtain domesticated bacterial liquid; the domesticated bacterial liquid is subjected to enlarged culture to obtain a functional enhanced bacterial agent, and the bacterial agent is added in batches before the pile fermentation and pit entry fermentation; through the synergistic effect of the precursor release and the microbial conversion, the application can significantly improve the content of base liquor flavoring substances, reduce the content of harmful by-products such as n-propanol, stably improve the liquor yield, and has high process integration degree and is easy to popularize and promote in the industry.

The invention belongs to the field of additives, and provides an animal feed additive and a preparation method thereof, and the animal feed additive is prepared by mixing a Chinese herbal medicine fermentation liquid and black gadfly pulp according to a mass ratio of 1: (200-500), the Chinese herbal medicine fermentation liquor is prepared by fermenting the following raw materials: 10-15 parts of astragalus membranaceus, 6-10 parts of liquorice, 8-10 parts of salvia miltiorrhiza, 10-12 parts of white paeony root, 10-15 parts of scutellaria baicalensis, 6-12 parts of rheum officinale, 8-12 parts of golden cypress, 10-15 parts of sapium sebiferum, 8-12 parts of tobacco and 4-6 parts of medicated leaven, and the raw materials are crushed and fermented with a compound microbial strain to obtain the Chinese herbal medicine fermentation liquor; the compound microbial strain comprises bacillus subtilis, lactic acid bacteria and bacillus licheniformis, and the viable count ratio of the bacillus subtilis to the lactic acid bacteria to the bacillus licheniformis is (2-3): (1-2): 1; the fermented Chinese herbal medicine is taken as a core, Chinese herbal medicine macromolecules are decomposed into micromolecular active substances (such as fermented astragalus polysaccharide) through microbial conversion of bacillus subtilis and the like, the drug effect is improved by 4-28 times, antibiotic residues are avoided, the problems of drug resistance and drug accumulation are avoided, and the food safety requirement is met.

The invention belongs to the technical field of biology, and particularly discloses application of glycosyl transferase PdUGT from apricot trees in biosynthesis of salidroside, and the sequence of the glycosyl transferase PdUGT is as shown in SEQ ID NO. 2. The gene coding SEQ ID NO.2 is transferred into bacillus licheniformis, tyrosol is added into a fermentation culture medium for fermentation, biologically fermented salidroside can be obtained, tyrosol can be efficiently and specifically converted to synthesize salidroside, 12 g / L of tyrosol can be converted to 23.92 g / L at most, and the conversion rate is larger than 92%. The invention lays a foundation for industrial production of salidroside by a microbial conversion method.

The application belongs to the technical field of medicine, and discloses a kind of 13, 28-epoxy substituted ussane type triterpenes and its preparation method and application. The application uses microbial transformation technology, with ursolic acid as substrate, and introduces epoxy group substitution in 13 and 28 positions with regional selectivity and stereoselectivity, and introduces hydroxyl, carbonyl or acetoxy in multiple positions of the parent nucleus, so that the novel structure of 13, 28-epoxy substituted ussane type triterpene derivatives formula I-IX is obtained. And through in vitro anti-tumor cell test, it is confirmed that compounds formula I-IX have significant anti-tumor activity, can be used as active ingredients of antitumor drugs, and have wide application.

The present invention relates to the field of pharmaceutical chemistry, and specifically to a method for preparing cholesterol, a derivative thereof, and an analog thereof. The derivative of cholesterol comprises, but is not limited to, 7-dehydrocholesterol, 25-hydroxycholesterol, 25-hydroxy-7-dehydrocholesterol, and ergosterol. In the present invention, a compound represented by formula I can be prepared by means of a microbial transformation using phytosterols as a raw material, followed by preparing cholesterol, the derivative thereof, and the analog thereof.

The invention discloses a method for preparing astragaloside IV by utilizing microbial conversion, and particularly relates to a method for improving the content of astragaloside IV in an astragalus membranaceus water extraction concentrated solution by utilizing bacillus subtilis and taking the astragalus membranaceus water extraction concentrated solution as a substrate. The obtained astragaloside is relatively high in purity, the operation is simple, no pollution is caused, and the reaction conditions are mild.

The invention relates to the technical field of biology, in particular to a phenylpyruvate decarboxylase ARO10 protein mutant and application of the phenylpyruvate decarboxylase ARO10 protein mutant in preparation of tyrosol. The invention discloses an ARO10 mutant. The phenylpyruvate decarboxylase mutant is obtained by mutating one or more of the 335th site of phenylpyruvate decarboxylase ARO10 (an amino acid sequence shown as SEQ ID NO1) from yeast. Experiments prove that the mutated phenylpyruvate decarboxylase protein can improve the specific reaction on 4-hydroxyphenylpyruvic acid, so that the tyrosol generation capability of saccharomyces cerevisiae is improved. Glycosyl transferase ARO10 mutant protein is expressed in microorganisms, the capacity of the microorganisms for converting tyrosol is obviously enhanced, and the industrial application prospect is good.

The application discloses Leporin compounds, a preparation method thereof and application of the Leporin compounds in preparation of anti-influenza virus drugs. Leporins B and C are separated from a solid fermentation extract of a strain CPCC 401592, and a new compound of formula III is obtained by microbial transformation of Leporin B. The application further discloses a method for separating Leporins B and C from the strain and a method for obtaining the Leporin compound of formula III by microbial transformation of Leporin B. The Leporin compounds provided by the application all show strong inhibitory effects on influenza A virus, and the inhibitory activities are higher than those of a positive control drug, so the Leporin compounds have application prospects in preparation of drugs or preparations for preventing or treating influenza virus infection.

The invention relates to the technical field of biology, in particular to a phenylpyruvate decarboxylase ARO10 protein mutant and application of the phenylpyruvate decarboxylase ARO10 protein mutant in preparation of tryptophol. The invention discloses an ARO10 mutant. The phenylpyruvate decarboxylase mutant is obtained by mutating one or more of the 335th amino acid, the 339th amino acid and the 628th amino acid of phenylpyruvate decarboxylase ARO10 (the amino acid sequence shown as SEQ ID NO.1) from yeast. Experiments prove that the mutated phenylpyruvate decarboxylase protein can improve the specific reaction on indole-3-pyruvic acid, so that the tryptophol generation capability of saccharomyces cerevisiae is improved. Glycosyl transferase ARO10 mutant protein is expressed in microorganisms, the tryptophol conversion capacity of the microorganisms is obviously enhanced, and the industrial application prospect is good.